Double-light-source three-channel single-particle biological aerosol detection system

Abstract

The embodiment of the invention discloses a double-light-source three-channel single-particle biological aerosol detection system. Comprising a first light source assembly used for forming first ultraviolet light; the second light source assembly is used for forming second ultraviolet light; the first dichroscope component is used for transmitting the first ultraviolet light and the second ultraviolet light in the same direction; the first plano-convex lens component is used for focusing the first ultraviolet light and the second ultraviolet light; the light collecting assembly is used for collecting scattered light and fluorescent light of the first ultraviolet light and the second ultraviolet light and outputting the scattered light and the fluorescent light; the second plano-convex lens component is used for enabling the light output from the light collecting component to form collimated light; the second dichroscope component is used for splitting the collimated light formed by the second plano-convex lens component; the first detection assembly is used for detecting reflected light of the second dichroscope; the third dichroscope component is used for splitting the transmission light of the second dichroscope; the second detection assembly is used for detecting reflected light of the third dichroscope; and the third detection assembly is used for detecting transmission light of the third dichroscope.

Description

Technical Field

[0001] The invention belongs to the technical field of bioaerosol detection, and in particular relates to a dual-light source three-channel single-particle bioaerosol detection system. Background Art

[0002] Bioaerosols in the troposphere can absorb shorter wavelength light, which can affect the ecological balance. Using lidar to remotely sense bioaerosols and determine their characteristics such as particle number density is one of the current research hotspots. Bioaerosols can produce fluorescence under the induction of lasers, while non-biological aerosols cannot. Therefore, aerosol bioaerosols can be detected by a lidar system based on laser-induced fluorescence.

[0003] For example, ultraviolet lasers can be used to induce fluorescence from fluorophores within bioparticle particles. These fluorophores include tryptophan, NADH, and flavin, and the observed fluorescence depends on the applied wavelength, usually in the range of 266 to 365 nm, as well as the biochemical fluorophores in the bioparticle. Tryptophan has no fluorescence under 365 nm wavelength excitation light, but can emit fluorescence proportional to the particle concentration under 280 nm wavelength excitation light, and the linear correlation coefficient of the fitted line is 0.995. The results show that the bioaerosol detection device has a good linear response relationship to tryptophan aerosol. In addition, the sensitivity and fluorescence efficiency of bioparticle detection using this system are reduced, probably because some tryptophan decomposes over time, loses biological activity, and cannot produce fluorescence.

[0004] It can be found that using a single 365nm light source for fluorescence excitation cannot fully cover the organic molecules, so during the bioaerosol particle detection process, there will be no response to some bioaerosol particles. Summary of the invention

[0005] In view of this, some embodiments disclose a dual-light source three-channel single-particle bioaerosol detection system, the system comprising:

[0006] A single-particle bioaerosol forming assembly for forming a bioaerosol to be detected;

[0007] A first light source assembly, used for forming a first ultraviolet light;

[0008] A second light source assembly, used for forming a second ultraviolet light;

[0009] A first dichroic mirror component is adapted to be arranged with the first light source assembly and the second light source assembly, and is used to transmit the first ultraviolet light and the second ultraviolet light in the same direction;

[0010] A first plano-convex mirror component is arranged in the output light direction of the first dichroic mirror component, and is used to focus the first ultraviolet light and the second ultraviolet light transmitted in the same direction onto the bioaerosol to be detected;

[0011] A light collecting component, comprising a spherical mirror component and an ellipsoidal mirror component which are arranged opposite to each other, wherein a lens is arranged at the center of the spherical mirror component; the spherical mirror component and the ellipsoidal mirror component are respectively arranged on both sides of the single-particle bioaerosol forming component, and are used to collect the first ultraviolet light, the second ultraviolet light reflected by the bioaerosol, and the first fluorescence induced by the first ultraviolet light and the second fluorescence induced by the second ultraviolet light of the single bioaerosol particle, and output them from the lens of the spherical mirror component;

[0012] A second plano-convex lens component is arranged outside the spherical mirror component and is used to form collimated light from the light output from the lens of the spherical mirror component;

[0013] A second dichroic mirror component is arranged corresponding to the second plano-convex lens component and is used for splitting the collimated light formed by the second plano-convex lens component;

[0014] A first detection assembly, disposed in the direction of the reflected light of the second dichroic mirror component, for detecting the reflected light of the second dichroic mirror;

[0015] A third dichroic mirror component is arranged in the direction of the transmitted light of the second dichroic mirror component and is used to split the transmitted light of the second dichroic mirror;

[0016] A second detection assembly is arranged in the direction of the reflected light of the third dichroic mirror component and is used to detect the reflected light of the third dichroic mirror;

[0017] A third detection assembly, disposed in the direction of the transmitted light of the third dichroic mirror component, for detecting the transmitted light of the third dichroic mirror;

[0018] When the system is applied to single-particle bioaerosol detection, the first light source assembly and the second light source assembly sequentially form the first ultraviolet light and the second ultraviolet light according to a set timing.

[0019] Furthermore, in some embodiments of the dual-light source three-channel single-particle bioaerosol detection system disclosed, the center position of the spherical mirror component is the focal position of the ellipsoidal mirror component.

[0020] Some embodiments disclose a dual-light source three-channel single-particle bioaerosol detection system, which also includes a light trap component, which is arranged on the outside of the single-particle bioaerosol forming component and corresponds to the first plano-convex lens, and is used to absorb the first ultraviolet light and the second ultraviolet light that penetrate the bioaerosol.

[0021] The dual-light source three-channel single-particle bioaerosol detection system disclosed in some embodiments also includes a control component;

[0022] The control component is connected to the first light source component and the second light source component, and is used to control the timing of forming the first ultraviolet light and the second ultraviolet light;

[0023] The control component is connected to the first detection component, the second detection component and the third detection component for processing detection information.

[0024] Some embodiments disclose a dual-light source three-channel single-particle bioaerosol detection system, wherein the first light source assembly includes a first ultraviolet laser lamp component and a plano-convex mirror arranged corresponding thereto; the first ultraviolet laser lamp component is used to form a first ultraviolet light with a wavelength of 280 nm.

[0025] In the dual-light source three-channel single-particle bioaerosol detection system disclosed in some embodiments, in the detection system, the optical path of the first ultraviolet light with a wavelength of 280nm includes:

[0026] After being reflected by the first dichroic mirror, the first ultraviolet light is focused by the first plano-convex lens, acts on the bioaerosol particles, and induces the generation of the first fluorescence; the first ultraviolet light and the first fluorescence reflected by the bioaerosol particles are focused by the interaction of the spherical mirror component and the ellipsoidal mirror component, and pass through the lens of the spherical mirror component, wherein the first ultraviolet light is totally reflected by the second dichroic mirror component and enters the first detection component, and the first fluorescence penetrates the second dichroic mirror component, is totally reflected by the third dichroic mirror component, and enters the second detection component.

[0027] In some embodiments of the disclosed dual-light source three-channel single-particle bioaerosol detection system, the second light source assembly includes a second ultraviolet laser lamp component and a plano-convex mirror arranged corresponding thereto, and the second ultraviolet laser lamp component is used to form a second ultraviolet light with a wavelength of 365nm.

[0028] In the dual-light source three-channel single-particle bioaerosol detection system disclosed in some embodiments, in the detection system, the optical path of the second ultraviolet light with a wavelength of 365 nm includes:

[0029] After being totally reflected by the first dichroic mirror, the second ultraviolet light is focused by the first plano-convex lens, acts on the bioaerosol particles, and induces the generation of a second fluorescence; the second ultraviolet light and the second fluorescence reflected by the bioaerosol particles are focused by the interaction between the spherical mirror component and the ellipsoidal mirror component, pass through the lens of the spherical mirror component, and are totally transmitted by the second dichroic mirror; wherein, the second ultraviolet light is totally reflected by the third dichroic mirror component, and enters the second detection component, and the second fluorescence is totally transmitted by the third dichroic mirror component, and enters the third detection component.

[0030] Some embodiments disclose a dual-light source three-channel single-particle bioaerosol detection system, and a method for detecting bioaerosol using the detection system includes:

[0031] First, the first light source component generates a first ultraviolet light with a wavelength of 280 nm. After the first ultraviolet light penetrates the first dichroic mirror component, it is focused by the first plano-convex mirror component and reaches the single-particle bioaerosol component, forming an irradiation focus point in the bioaerosol formed therein; the induced first fluorescence enters the second plano-convex lens component to form collimated light under the action of the light collecting component, penetrates the second dichroic mirror component, is totally reflected by the third dichroic mirror component, and is received by the second detection component; the reflected first ultraviolet light enters the second plano-convex lens component to form collimated light under the action of the light collecting component, is totally reflected by the second dichroic mirror component, and is received by the first detection component;

[0032] Then, the second light source component forms a second ultraviolet light with a wavelength of 365nm. After being totally reflected by the first dichroic mirror component, the second ultraviolet light is focused by the first plano-convex lens component and reaches the single-particle bioaerosol component, forming an irradiation focus point in the bioaerosol formed therein; the induced second fluorescence enters the second plano-convex lens component to form collimated light under the action of the collecting component, and after being totally penetrated from the second dichroic mirror component, it is further totally transmitted by the third dichroic mirror component and received by the third detection component; the reflected second ultraviolet light enters the second plano-convex lens component to form collimated light under the action of the light collecting component, and is totally transmitted from the second dichroic mirror component, and then totally reflected by the third dichroic mirror component and received by the second detection component;

[0033] In the above process, the optical signals respectively received by the first detection component, the second detection component and the third detection component are further processed to obtain the detection results of the bioaerosol.

[0034] In some embodiments of the disclosed dual-light source three-channel single-particle bioaerosol detection system, the single-particle bioaerosol is configured to form a component configured to form a columnar bioaerosol.

[0035] The dual-light source three-channel single-particle bioaerosol detection system disclosed in the embodiment of the present invention utilizes two light source components to form two types of ultraviolet light with wavelengths of 280nm and 365nm respectively, and controls the two types of ultraviolet light to irradiate the bioaerosol respectively in accordance with a set sequence, utilizes three optical channels to respectively detect two types of external light and two bands of fluorescence induced by the two types of ultraviolet light, and further realizes effective detection of biological particles through data processing; the detection system has low stray light and high signal-to-noise ratio, effectively enhances the detection range of biological particles, can effectively detect a variety of biological particles, and has good application prospects in the field of bioaerosol detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 , one A schematic diagram of the composition of a dual-light source three-channel single-particle bioaerosol detection system disclosed in some embodiments;

[0037] Figure 2 , one Schematic diagram of components of the dual-light source three-channel single-particle bioaerosol detection system disclosed in some embodiments Figure 1 ;

[0038] Figure 3 , one Schematic diagram of components of the dual-light source three-channel single-particle bioaerosol detection system disclosed in some embodiments Figure 2 .

[0039] Reference numerals

[0040] 1 Aerosol forming component 2 First light source component

[0041] 3 Second light source assembly 4 First dichroic mirror assembly

[0042] 5 first plano-convex mirror component 6 light collecting component

[0043] 7 Second plano-convex lens component 8 Second dichroic mirror component

[0044] 9 The third dichroic mirror component 10 The first detection component

[0045] 11 second detection component 12 third detection component

[0046] 20 First UV laser lamp 21 First light source plano-convex lens

[0047] 30 second ultraviolet laser lamp 31 second light source plano-convex lens

[0048] 60 Ellipsoidal mirror component 61 Spherical mirror component

[0049] 611 Lens 100 I Plano-Convex Lens

[0050] 101 No. I photodetector 110 No. II plano-convex mirror

[0051] 111 Photodetector No. II 120 Plano-convex mirror No. III

[0052] 121 III Photodetector 13 Light Trap DETAILED DESCRIPTION

[0053] The word "embodiment" used herein as an "exemplary" does not necessarily mean that any embodiment described is superior or better than other embodiments. Performance index tests in the embodiments of the present invention are performed using conventional test methods in the art unless otherwise specified. It should be understood that the terms described in the embodiments of the present invention are only used to describe specific implementation methods and are not intended to limit the contents disclosed in the embodiments of the present invention.

[0054] Unless otherwise specified, the technical and scientific terms used herein have the same meanings as commonly understood by ordinary technicians in the technical field to which the embodiments of the present invention belong; other experimental methods and technical means not specifically specified in the embodiments of the present invention refer to experimental methods and technical means commonly used by ordinary technicians in the field.

[0055] The terms "substantially" and "approximately" used herein are used to describe small fluctuations. For example, they can refer to less than or equal to ±5%, such as less than or equal to ±2%, such as less than or equal to ±1%, such as less than or equal to ±0.5%, such as less than or equal to ±0.2%, such as less than or equal to ±0.1%, such as less than or equal to ±0.05%. The numerical data represented or presented in the range format herein are used only for convenience and brevity, and should therefore be flexibly interpreted as including not only the values ​​clearly listed as the limits of the range, but also all independent values ​​or sub-ranges contained in the range. For example, the numerical range of "1-5%" should be interpreted as including not only the clearly listed values ​​of 1% to 5%, but also the independent values ​​and sub-ranges within the range shown. Therefore, independent values ​​such as 2%, 3.5% and 4% and sub-ranges such as 1%-3%, 2%-4% and 3%-5% are included in this numerical range. This principle also applies to the range of only one numerical value. In addition, such an interpretation applies regardless of the width of the range or the characteristics described.

[0056] In this document, including in the claims, transitional words such as "comprises," "includes," "with," "having," "containing," "involving," "accommodating," etc. are understood to be open-ended, i.e., meaning "including but not limited to." Only the transitional words "consisting of" and "composed of" are closed transitional words.

[0057] In order to better illustrate the present invention, numerous specific details are provided in the specific examples below. It should be understood by those skilled in the art that the present invention can be implemented without certain specific details. In the embodiments, some methods, means, instruments, equipment, etc. well known to those skilled in the art are not described in detail in order to highlight the gist of the present invention.

[0058] Under the premise of no conflict, the technical features disclosed in the embodiments of the present invention may be arbitrarily combined, and the resulting technical solutions belong to the contents disclosed in the embodiments of the present invention.

[0059] In some embodiments, Figure 1 , Figure 2 , Figure 3 As shown, the dual-light source three-channel single-particle bioaerosol detection system includes:

[0060] A single-particle bioaerosol forming component 1, used to form a bioaerosol to be detected;

[0061] The first light source assembly 2 is used to form a first ultraviolet light; the first light source assembly 2 includes a first ultraviolet laser lamp 20 and a first light source plano-convex lens 21 arranged corresponding thereto; the first ultraviolet laser lamp 20 is used to form a first ultraviolet light with a wavelength of 280 nm;

[0062] The second light source assembly 3 is used to form a second ultraviolet light; the second light source assembly 3 includes a second ultraviolet laser lamp 30 and a second light source plano-convex lens 31 arranged corresponding thereto, and the second ultraviolet laser lamp 30 is used to form a second ultraviolet light with a wavelength of 365nm; when the detection system is applied to single-particle bioaerosol detection, the first light source assembly 2 and the second light source assembly 3 sequentially form the first ultraviolet light and the second ultraviolet light according to a set timing;

[0063] The first dichroic mirror component 4 is adapted to be arranged with the first light source assembly 2 and the second light source assembly 3, and is used to transmit the first ultraviolet light and the second ultraviolet light in the same direction; generally, the first dichroic mirror component 4 has a higher reflectivity to the short-wave 280nm ultraviolet laser and a higher transmittance to the long-wave 365nm ultraviolet laser;

[0064] A first plano-convex mirror component 5 is arranged in the output light direction of the first dichroic mirror component 4, and is used to focus the first ultraviolet light and the second ultraviolet light transmitted in the same direction onto the bioaerosol to be detected;

[0065] The light collecting component 6 comprises a spherical mirror component 61 and an ellipsoidal mirror component 62 which are arranged opposite to each other, and a lens 611 is arranged at the center of the spherical mirror component 61; generally, the lens at the center of the spherical mirror can ensure full light transmission, while other parts of the spherical mirror outside the center can ensure full light reflection; the spherical mirror component 61 and the ellipsoidal mirror component 62 are respectively arranged on both sides of the single-particle bioaerosol forming component 1, and are used to collect the first ultraviolet light, the second ultraviolet light reflected by the bioaerosol, and the first fluorescence induced by the first ultraviolet light and the second fluorescence induced by the second ultraviolet light of the single bioaerosol particle, and output them from the lens 611 of the spherical mirror component 61; generally, the center of the spherical mirror component 61 is the focal position of the ellipsoidal mirror component 62;

[0066] The second plano-convex lens component 7 is disposed outside the spherical mirror component 61 and is used to form collimated light from the light output from the lens 611 of the spherical mirror component 61;

[0067] The second dichroic mirror component 8 is arranged corresponding to the second plano-convex lens component 7, and is used for splitting the collimated light formed by the second plano-convex lens component 7; generally, the second dichroic mirror component 8 has a higher reflectivity to the 280nm ultraviolet laser of the Mi signal, and has a higher transmittance to the first fluorescence induced by the 280nm ultraviolet laser and the 365nm ultraviolet laser of another channel and the second fluorescence induced by the 365nm ultraviolet laser of the Mi signal;

[0068] The first detection assembly 10 is arranged in the direction of the reflected light of the second dichroic mirror component 8, and is used to detect the reflected light of the second dichroic mirror 8; the first detection assembly 10 includes a plano-convex mirror No. 100 and a photoelectric detector No. 101 arranged corresponding to the plano-convex mirror 100, and the photoelectric detector No. 101 is adapted to be provided with an aperture;

[0069] The third dichroic mirror component 9 is arranged in the transmission light direction of the second dichroic mirror component 8, and is used for splitting the transmission light of the second dichroic mirror component 8; when the 280nm ultraviolet laser induces fluorescence, the third dichroic mirror component 9 usually collects the induced first fluorescence in two parts, the fluorescence has a higher reflectivity in the short wave, and the fluorescence has a higher transmittance in the long wave; and for the second fluorescence induced by the 365nm ultraviolet laser, the third dichroic mirror component 9 has a higher reflectivity for the 365nm ultraviolet laser signal, and has a higher transmittance for the full band of the fluorescence generated by it;

[0070] The second detection assembly 11 is arranged in the direction of the reflected light of the third dichroic mirror component 9, and is used to detect the reflected light of the third dichroic mirror component 9; the second detection assembly 11 includes a plano-convex mirror No. II 110 and a photoelectric detector No. II 111 arranged corresponding thereto, and the photoelectric detector No. II 111 is adapted to be provided with an aperture;

[0071] The third detection assembly 12 is arranged in the transmission light direction of the third dichroic mirror component 9, and is used to detect the transmission light of the third dichroic mirror component 9; the third detection assembly 12 includes a plano-convex mirror No. III 120 and a photoelectric detector No. III 121 arranged corresponding thereto, and the photoelectric detector No. III 121 is adapted to be provided with an aperture;

[0072] The light trap component 13 is arranged outside the single-particle bioaerosol forming component 1, and is arranged corresponding to the first plano-convex lens 5, and is used to absorb the first ultraviolet light and the second ultraviolet light that penetrate the bioaerosol. Ultraviolet light usually scatters around the biological particles in the bioaerosol, among which the stray light passing along the direction of the ultraviolet light is the strongest. Absorbing it with a light trap can prevent this part of the light from being scattered multiple times inside the light collection component, and prevent it from causing certain effects on the system detection signal.

[0073] In some embodiments, the single-particle bioaerosol assembly includes a structure for forming an air path channel, and the air path is divided into two air flows, a sheath flow and a sample flow. The sheath flow is clean air that passes through a filter. Before the clean air of the air pump is mixed with the sample flow, a portion of the air is first released through an exhaust valve. The released air is equal to the contaminated air inhaled into the sheath flow. The sheath flow will wrap the sample flow, and through the action of air pressure, the sample flow will be pulled into a filament to achieve a single-particle flow control effect on the particles in the sample flow. Finally, the sample flow is focused on the center point by the irradiation of an ultraviolet light source under the protection of the sheath flow to achieve fluorescence excitation of microorganisms. The air passes through the filter element, which will filter out impurities above 0.02 microns, and is also used to smooth the air pressure pulse fluctuations generated by the air pump. The air pump provides power for the flow of gas, not only inhaling gas from the inlet, but also generating a suction force on the gas in the sheath tube. The sheath flow rate is obtained according to the experiment, and the external flowmeter adjusts the duty cycle of the PWM to control the air pump to generate a corresponding suction force, thereby controlling the sheath flow rate.

[0074] The dual-light source three-channel single-particle bioaerosol detection system disclosed in some embodiments also includes a control component;

[0075] The control component is connected to the first light source component and the second light source component, and is used to control the timing of forming the first ultraviolet light and the second ultraviolet light;

[0076] The control component is connected to the first detection component, the second detection component and the third detection component for processing detection information. Usually, the ultraviolet laser irradiates the bioaerosol gas to be detected, and finally generates a scattered light signal and a fluorescence signal after passing through multiple optical components. The photomultiplier tube PMT generates a corresponding current after receiving the light signal. The current is converted into voltage, filtered, and amplified to form a pulse waveform. The pulse waveform is sampled by ADC, the analog signal is converted into a data signal, and the properties of the gas are judged by combining the intensity signal and the fluorescence signal.

[0077] In some embodiments, the control component includes an FPGA module; the photodetector signals of the last three channels arrive at the FPGA for algorithm calculation and are stored in the SOC. When the number of fluorescent signal particles (at least one channel) collected by the second photodetector and the third photodetector calculated by the FPGA exceeds the set threshold, the system alarms.

[0078] In the dual-light source three-channel single-particle bioaerosol detection system disclosed in some embodiments, in the detection system, the optical path of the first ultraviolet light with a wavelength of 280nm includes:

[0079] The first light source component 2 reflects the first ultraviolet light, and after being reflected by the first dichroic mirror component 4, the first ultraviolet light is focused by the first plano-convex lens component 5, acts on the bioaerosol particles, and induces the generation of the first fluorescence; the first ultraviolet light and the first fluorescence reflected by the bioaerosol particles are focused under the interaction of the spherical mirror component 61 and the ellipsoidal mirror component 62, pass through the lens 611 of the spherical mirror component 61, and pass through the second plano-convex lens component 7 to form collimated light, wherein the first ultraviolet light is totally reflected by the second dichroic mirror component 8, and enters the first detection component 10, and the first fluorescence penetrates the second dichroic mirror component 8, is totally reflected by the third dichroic mirror component 9, and enters the second detection component 11;

[0080] The optical path of the second ultraviolet light with a wavelength of 365nm includes:

[0081] The second light source component 3 reflects the second ultraviolet light, and after the second ultraviolet light is totally reflected by the first dichroic mirror component 4, it is focused by the first plano-convex lens component 5, acts on the bioaerosol particles, and induces the generation of a second fluorescence; the second ultraviolet light and the second fluorescence reflected by the bioaerosol particles are focused by the interaction between the spherical mirror component 61 and the ellipsoidal mirror component 62, pass through the lens 611 of the spherical mirror component 61, pass through the second plano-convex lens component 7 to form a collimated light, and are totally transmitted by the second dichroic mirror component 8; wherein, the second ultraviolet light is totally reflected by the third dichroic mirror component 9, and enters the second detection component 11, and the second fluorescence is totally transmitted by the third dichroic mirror component 9, and enters the third detection component 12.

[0082] Some embodiments disclose a dual-light source three-channel single-particle bioaerosol detection system, and a method for detecting bioaerosol using the detection system includes:

[0083] First, the first light source component generates a first ultraviolet light with a wavelength of 280nm. After penetrating the first dichroic mirror component 4, the first ultraviolet light is focused by the first plano-convex mirror component 5 and reaches the single-particle bioaerosol component, forming a focusing point in the bioaerosol. The first fluorescence generated by the induced bioparticles enters the second plano-convex lens component 7 to form collimated light under the action of the light collecting component 6, penetrates the second dichroic mirror component 8, is totally reflected by the third dichroic mirror component 9, and is received by the second detection component 11. The reflected first ultraviolet light enters the second plano-convex lens component 7 to form collimated light under the action of the light collecting component 6, is totally reflected by the second dichroic mirror component 8, and is received by the first detection component 10.

[0084] Then, the second light source component forms a second ultraviolet light with a wavelength of 365nm. After being totally reflected by the first dichroic mirror component 4, the second ultraviolet light is focused by the first plano-convex lens component 5 and is focused in the bioaerosol formed by the single-particle bioaerosol component; the second fluorescence generated by the induced biological particles enters the second plano-convex lens component 7 to form collimated light under the action of the collecting component 6, and after being totally penetrated by the second dichroic mirror component 8, it is further totally transmitted by the third dichroic mirror component 9 and is received by the third detection component 12; the reflected second ultraviolet light enters the second plano-convex lens component 7 to form collimated light under the action of the light collecting component 6, and is totally transmitted by the second dichroic mirror component 8, and then is totally reflected by the third dichroic mirror component 9 and is received by the second detection component 11;

[0085] In the above process, the optical signals respectively received by the first detection component 10, the second detection component 11, and the third detection component 12 are further processed to obtain the detection results of the biological particles in the bioaerosol.

[0086] The first fluorescence excited by the first ultraviolet light of 280nm is collected by the second detection component and the third detection component, covering a relatively wide fluorescence band; the second fluorescence excited by the second ultraviolet light of 365nm is collected by the second detection component. According to the proportional relationship between the fluorescence intensity and the band generated by the first ultraviolet light of 280nm and the second ultraviolet light of 365nm, the corresponding extinction coefficient can be calculated, and the number concentration of bioaerosol particles can be obtained.

[0087] The dual-light source three-channel single-particle bioaerosol detection system disclosed in the embodiment of the present invention utilizes two light source components to form two ultraviolet lasers with wavelengths of 280nm and 365nm respectively, and controls the two ultraviolet lasers to irradiate the bioaerosol respectively in sequence according to a set timing, utilizes three optical channels to detect two external lights and two bands of fluorescence induced by the two ultraviolet lasers respectively, and further realizes effective detection of biological particles through data processing; the detection system has low stray light and high signal-to-noise ratio, effectively enhances the detection range of biological particles, can effectively detect a variety of biological particles, and has good application prospects in the field of bioaerosol detection.

[0088] The technical solutions disclosed in the embodiments of the present invention and the technical details disclosed in the embodiments are merely illustrative of the inventive concept of the present invention and do not constitute a limitation on the technical solutions of the embodiments of the present invention. Any conventional changes, replacements or combinations of the technical details disclosed in the embodiments of the present invention have the same inventive concept as the present invention and are within the protection scope of the claims of the present invention.

Claims

1. Dual-light source three-channel single-particle bioaerosol detection system, characterized by: include: A single-particle bioaerosol forming assembly for forming a bioaerosol to be detected; A first light source assembly, used for forming a first ultraviolet light; A second light source assembly, used for forming a second ultraviolet light; A first dichroic mirror component, adapted to be arranged with the first light source assembly and the second light source assembly, and used for transmitting the first ultraviolet light and the second ultraviolet light in the same direction; a first plano-convex mirror component, arranged in the output light direction of the first dichroic mirror component, and used for focusing the first ultraviolet light and the second ultraviolet light transmitted in the same direction onto the bioaerosol to be detected; A light collecting component, comprising a spherical mirror component and an ellipsoidal mirror component arranged opposite to each other, wherein a lens is arranged at the center of the spherical mirror component; the spherical mirror component and the ellipsoidal mirror component are respectively arranged on both sides of the single-particle bioaerosol forming component; and is used to collect the first ultraviolet light, the second ultraviolet light reflected by the bioaerosol, and the first fluorescence induced by the first ultraviolet light and the second fluorescence induced by the second ultraviolet light of the single bioaerosol particle, and output them from the lens of the spherical mirror component; A second plano-convex lens component is arranged outside the spherical mirror component and is used to form collimated light from the light output from the lens of the spherical mirror component; a second dichroic mirror component, arranged corresponding to the second plano-convex lens component, and used for splitting the collimated light formed by the second plano-convex lens component; A first detection component, arranged in the direction of the reflected light of the second dichroic mirror component, for detecting the reflected light of the second dichroic mirror; a third dichroic mirror component, arranged in the direction of the transmitted light of the second dichroic mirror component, and used for splitting the transmitted light of the second dichroic mirror; A second detection component, arranged in the direction of the reflected light of the third dichroic mirror component, for detecting the reflected light of the third dichroic mirror; A third detection assembly, arranged in the direction of the transmitted light of the third dichroic mirror component, for detecting the transmitted light of the third dichroic mirror; When the detection system is applied to single-particle bioaerosol detection, the first light source assembly and the second light source assembly sequentially generate the first ultraviolet light and the second ultraviolet light according to a set timing.

2. The dual-light source three-channel single-particle bioaerosol detection system according to claim 1 is characterized in that: The center of the spherical mirror component is the focal position of the ellipsoidal mirror component.

3. The dual-light source three-channel single-particle bioaerosol detection system according to claim 1 is characterized in that: It also includes a light trap component, which is arranged on the outside of the single-particle bioaerosol forming component and is corresponding to the first plano-convex lens, and is used to absorb the first ultraviolet light and the second ultraviolet light that penetrate the bioaerosol.

4. The dual-light source three-channel single-particle bioaerosol detection system according to claim 1 is characterized in that: Also included are control components; The control component is connected to the first light source component and the second light source component, and is used to control the timing of forming the first ultraviolet light and the second ultraviolet light; The control component is connected to the first detection component, the second detection component and the third detection component for processing detection information.

5. The dual-light source three-channel single-particle bioaerosol detection system according to claim 1 is characterized in that: The first light source assembly includes a first ultraviolet laser lamp component and a plano-convex mirror arranged corresponding to the first ultraviolet laser lamp component; the first ultraviolet laser lamp component is used to form a first ultraviolet light with a wavelength of 280nm.

6. The dual-light source three-channel single-particle bioaerosol detection system according to claim 5, characterized in that: In the detection system, the optical path of the first ultraviolet light with a wavelength of 280nm includes: After being reflected by the first dichroic mirror, the first ultraviolet light is focused by the first plano-convex lens, acts on the bioaerosol particles, and induces the generation of the first fluorescence; the first ultraviolet light and the first fluorescence reflected by the bioaerosol particles are focused by the interaction of the spherical mirror component and the ellipsoidal mirror component, and pass through the lens of the spherical mirror component, wherein the first ultraviolet light is totally reflected by the second dichroic mirror component and enters the first detection component, and the first fluorescence penetrates the second dichroic mirror component, is totally reflected by the third dichroic mirror component, and enters the second detection component.

7. The dual-light source three-channel single-particle bioaerosol detection system according to claim 1 is characterized in that: The second light source assembly includes a second ultraviolet laser lamp component and a plano-convex mirror arranged correspondingly thereto, and the second ultraviolet laser lamp component is used to form a second ultraviolet light with a wavelength of 365 nm.

8. The dual-light source three-channel single-particle bioaerosol detection system according to claim 7, characterized in that: In the detection system, the optical path of the second ultraviolet light with a wavelength of 365nm includes: After being totally reflected by the first dichroic mirror, the second ultraviolet light is focused by the first plano-convex lens, acts on the bioaerosol particles, and induces the generation of a second fluorescence; the second ultraviolet light and the second fluorescence reflected by the bioaerosol particles are focused by the interaction between the spherical mirror component and the ellipsoidal mirror component, pass through the lens of the spherical mirror component, and are totally transmitted by the second dichroic mirror; wherein, the second ultraviolet light is totally reflected by the third dichroic mirror component, and enters the second detection component, and the second fluorescence is totally transmitted by the third dichroic mirror component, and enters the third detection component.

9. The dual-light source three-channel single-particle bioaerosol detection system according to claim 1, characterized in that: The method for detecting bioaerosol using the detection system comprises: First, the first light source component generates a first ultraviolet light with a wavelength of 280 nm. After the first ultraviolet light penetrates the first dichroic mirror component, it is focused by the first plano-convex mirror component and reaches the single-particle bioaerosol component, forming an irradiation focus point in the bioaerosol formed therein; the induced first fluorescence enters the second plano-convex lens component to form collimated light under the action of the light collecting component, penetrates the second dichroic mirror component, is totally reflected by the third dichroic mirror component, and is received by the second detection component; the reflected first ultraviolet light enters the second plano-convex lens component to form collimated light under the action of the light collecting component, is totally reflected by the second dichroic mirror component, and is received by the first detection component; Then, the second light source component forms a second ultraviolet light with a wavelength of 365nm. After being totally reflected by the first dichroic mirror component, the second ultraviolet light is focused by the first plano-convex lens component and reaches the single-particle bioaerosol component, and forms an irradiation focus point with the bioaerosol formed therein; the induced second fluorescence enters the second plano-convex lens component to form collimated light under the action of the collecting component, and after being totally penetrated from the second dichroic mirror component, it is further totally transmitted by the third dichroic mirror component and received by the third detection component; the reflected second ultraviolet light enters the second plano-convex lens component to form collimated light under the action of the light collecting component, and is totally transmitted from the second dichroic mirror component, and then totally reflected by the third dichroic mirror component and received by the second detection component; In the above process, the optical signals respectively received by the first detection component, the second detection component and the third detection component are further processed to obtain the detection results of the bioaerosol.

10. The dual-light source three-channel single-particle bioaerosol detection system according to claim 1, characterized in that: The single-particle bioaerosol forming assembly is configured to form a columnar bioaerosol.

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