Method for promoting corn growth and improving resistance of plants to northern corn leaf blight and application of method

Abstract

The invention relates to the technical field of microorganisms, in particular to a method for promoting corn growth to improve the resistance of plants to northern leaf blight of corn and application thereof.The method for promoting corn growth to improve the resistance of plants to northern leaf blight of corn comprises the following specific steps that S1, a test field is subjected to scribing treatment and divided into four test fields, each test field is divided into four ridges, the ridge width is 0.61 m, and the ridge width is 0.6 m; the ridge length is 15 m, the aisle between each cell is 2 m, and the front and rear protection rows of the test area are 10 m respectively; s2, a first area is a blank control area for normal planting of corn plants, a second area is a second area for drip irrigation of beauveria bassiana planting (Bb) for the corn plants, a third area is a third area for northern leaf blight inoculation planting (Et) for the corn plants, and a fourth area is a fourth area for drip irrigation of beauveria bassiana planting (Bb + Et) after northern leaf blight inoculation for the corn plants. Beauveria bassiana applied in a drip irrigation mode has good effects on corn growth and resistance.

Description

Technical Field

[0001] The invention relates to the technical field of microorganisms, and in particular to a method for promoting corn growth and improving plant resistance to corn leaf blight and an application thereof. Background Art

[0002] Corn is an important food crop and feed crop with great production potential and high economic benefits. It has edible, feed and multiple industrial uses. Corn is not only an important food crop, but also an important feed crop and industrial raw material. In addition, corn has high comprehensive utilization value. It is rich in protein and dietary fiber, which helps promote gastrointestinal motility and prevent constipation. Corn is also rich in vitamins and minerals, which helps improve human immunity and promote physical health.

[0003] Corn leaf spot is also known as streak disease and coal streak disease. The pathogen is a genus of Ascomycetes in the sexual state and a genus of asexual fungi, Helminthosporium, and is a major disease in corn production. Most corns planted on a large scale are susceptible hybrids. Corn leaf spot disease continues to spread and has the characteristics of short epidemic cycle, high frequency of occurrence, and expansion of the affected area, which has a great impact on the yield and quality of corn. Climate conditions are an important factor in the spread of corn leaf spot disease. During the heading and milky stage of the plant, the temperature is 20-28℃, and the humidity is 90% high. The pathogen can reproduce rapidly, produce a large number of conidia, and spread by wind.

[0004] Beauveria bassiana has a wide host range and is an effective microorganism for controlling arthropod pests. It has been widely used around the world. Recent studies have found that Beauveria bassiana has a certain antagonistic ability against a variety of soil-borne pathogens and leaf disease pathogens, and is a dual biocontrol fungus. Beauveria bassiana can inhibit the growth of a variety of pathogens such as wheat take-all pathogen, Fusarium oxysporum and Rhizoctonia solani. Summary of the invention

[0005] The object of the present invention is to provide a method for promoting corn growth and improving the resistance of the plant to corn leaf blight and its application, so as to solve the problems raised in the above background technology. To achieve the above object, the present invention provides the following technical solution: a method for promoting corn growth and improving the resistance of the plant to corn leaf blight, comprising the following specific steps:

[0006] S1. The experimental field was divided into four plots, each plot was divided into 4 ridges, the ridge width was 0.61m, the ridge length was 15m, the aisle between each plot was 2m, and the protection rows in front and behind the experimental area were 10m each;

[0007] S2, the first area is a blank control area where corn plants are planted normally (Control), the second area is a corn planted with Beauveria bassiana under drip irrigation (Bb), the third area is a corn planted with Euphorbia turcica (Et), and the fourth area is a corn planted with Beauveria bassiana after Euphorbia turcica (Bb+Et);

[0008] S3. Record the plant height, stem diameter, and chlorophyll of corn at each growth stage, and record the dry and wet weight of the aboveground and underground parts at the harvest stage. Take leaf samples during the main disease occurrence period, and use the qPCR method to detect the pathogen content in the leaves;

[0009] S4. Determine the grain yield. Take 10 corn plants from each plot, harvest corn ears, weigh them after killing and drying, and analyze the yield components such as thousand-grain weight and grain ear weight;

[0010] S5. Compare all the data comprehensively to finally determine whether biocontrol microorganisms can promote corn growth and improve the plant's resistance to southern corn leaf blight.

[0011] Preferably, the corn plants are artificially inoculated with southern corn leaf blight at the flaring stage.

[0012] Preferably, the dosage of the high spore pollen preparation per plot is 30 g (each plant is irrigated with 50 mL of 10^8 spores / mL inoculant, and the Beauveria content in the inoculant is 500 10^8 spores / g), the Beauveria preparation is evenly mixed with clean water, added to a small drip irrigation tank (volume 25-30 L), and irrigated using drip irrigation facilities.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] The microbial agent prepared by the method of the present invention is used in combination with water-fertilizer integrated equipment, which is convenient and quick.

[0015] The microbial agent prepared by the method of the present invention shows good effects on corn growth and resistance when Beauveria bassiana is applied in a drip irrigation manner. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 The effect of Beauveria bassiana on the chlorophyll content of corn plants;

[0017] Figure 2 The effect of Beauveria bassiana on the stem diameter of corn plants;

[0018] Figure 3 Effects of Beauveria bassiana on maize plant height

[0019] Figure 4 The effect of Beauveria bassiana on maize biomass (the upper figure shows the above-ground and underground dry weight of maize at full maturity, and the lower figure shows the state of roots at full maturity with different treatments);

[0020] Figure 5 is the incidence of corn leaf blight in different survey periods;

[0021] Figure 6 It is the disease index of northern corn leaf blight at the mature stage of corn;

[0022] In the figure, A is the corn seedling stage, B is the jointing stage, C is the tasseling and silking stage, and D is the milky stage. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technical personnel in this field without creative work are within the scope of protection of the present invention.

[0024] See also Figures 1 to 3 The present invention provides a technical solution: a method for promoting corn growth and improving plant resistance to corn leaf blight, comprising the following specific steps:

[0025] S1. The experimental field was divided into four plots, each plot was divided into 4 ridges, the ridge width was 0.61m, the ridge length was 15m, the aisle between each plot was 2m, and the protection rows in front and behind the experimental area were 10m each;

[0026] S2, the first area is a blank control area where corn plants are planted normally (Control), the second area is a corn planted with Beauveria bassiana under drip irrigation (Bb), the third area is a corn planted with Euphorbia turcica (Et), and the fourth area is a corn planted with Beauveria bassiana after Euphorbia turcica (Bb+Et);

[0027] S3. Record the plant height, stem diameter, and chlorophyll of corn at each growth stage, and record the dry and wet weight of the aboveground and underground parts at the harvest stage. Take leaf samples during the main disease occurrence period, and use the qPCR method to detect the pathogen content in the leaves;

[0028] S4. Determine the grain yield. Take 10 corn plants from each plot, harvest corn ears, weigh them after killing and drying, and analyze the yield components such as thousand-grain weight and grain ear weight;

[0029] S5. Compare all the data comprehensively to finally determine whether biocontrol microorganisms can promote corn growth and improve the plant's resistance to southern corn leaf blight.

[0030] In this embodiment, corn plants were artificially inoculated with southern corn leaf blight at the flaring stage.

[0031] In this embodiment, the incidence of corn leaf blight was calculated from the time when dark brown spots began to appear on the edges of corn leaves and light brown spots began to appear in the center 21 days after the corn leaf blight spore suspension was inoculated. 50 corn plants were surveyed in each treatment group using the five-point sampling method. The calculation formula for the incidence of corn leaf blight was as follows:

[0032] The incidence of corn leaf blight (%) = the total number of corn plants infected in each treatment / the total number of corn plants investigated × 100.

[0033] In this embodiment, the dosage of the high spore pollen preparation per plot is 30 g (each plant is irrigated with 50 mL of 10^8 spores / mL inoculant, and the Beauveria content in the inoculant is 50010^8 spores / g), the Beauveria preparation is mixed evenly with clean water, added to a small drip irrigation tank (volume 25-30 L), and irrigated using drip irrigation facilities.

[0034] In this embodiment, the microbial agent was applied once every 3 days starting from the seedling stage, for a total of 3 times.

[0035] In this embodiment, the effect of Beauveria bassiana on the chlorophyll content of corn is as follows: Figure 1 As shown in the figure, after corn plants were inoculated with Beauveria bassiana, the chlorophyll content in the leaves would change over time. In the natural disease-infected treatment group, the chlorophyll content of the biocontrol bacteria treatment group at each growth stage of corn was significantly higher than that of the control group; in the artificial disease-inoculated treatment group, the chlorophyll content of the biocontrol bacteria treatment group was higher than that of the control group starting from the tasseling and silking stage of corn.

[0036] In this embodiment, the effect of Beauveria bassiana on corn stem diameter is as follows: Figure 2 As shown in the figure, after corn plants were inoculated with Beauveria bassiana, the stem diameter of corn plants would change over time. In the natural disease-infected treatment group, the stem diameter of the biocontrol bacteria-treated group was significantly higher than that of the control group starting from the jointing stage; in the artificial disease-inoculated treatment group, the stem diameter of the Beauveria bassiana-treated group was higher than that of the control group ( Figure 2 ).

[0037] In this embodiment, the effect of Beauveria bassiana on the height of corn plants is as follows: Figure 3 As shown in the figure, after corn plants were inoculated with Beauveria bassiana and Tularemia viridis from the beginning of the tasseling and silking stage, the height of corn plants would change over time. In the tasseling and silking stage, the plant height of the Beauveria bassiana treatment group increased compared with the control group in the natural disease treatment group; in the milky stage of corn, the plant height of the biocontrol bacteria treatment group was lower than that of the control group under both natural disease and artificial inoculation conditions.

[0038] In this embodiment, the effect of Beauveria bassiana on corn biomass is as follows: Figure 4As shown in the figure, at the milky stage of corn, after corn plants were inoculated with Beauveria bassiana and Talaromyces purpurogenus, the aboveground and underground biomass of corn showed an increasing trend under natural disease and artificial inoculation conditions. Among them, the underground biomass of corn in the Beauveria bassiana treatment group was significantly higher than that in the control group under natural disease conditions, and the aboveground biomass of corn in the Beauveria bassiana treatment group was significantly higher than that in the control group under artificial inoculation conditions.

[0039] In this example, the effect of Beauveria bassiana on the incidence of corn leaf blight is shown in Figure 2. Figure 5 As shown, under artificial inoculation conditions, Beauveria bassiana can reduce the incidence of northern corn blight to a certain extent during the milky and mature stages of corn. At the mature stage of corn, the incidence of northern corn blight in the Beauveria bassiana-treated group was lower than that in the control group.

[0040] In this example, the effect of Beauveria bassiana on the disease index of corn leaf blight is as follows: Figure 6 As shown in the figure, at the milky stage of corn, under both natural infection and artificial inoculation conditions, the disease index of corn leaf blight in the Beauveria bassiana treatment group decreased significantly.

[0041] The test results confirmed the growth-promoting effect of the biocontrol microorganism, Beauveria bassiana, on corn plants and its field control effect on corn leaf blight. The results showed that the application of Beauveria bassiana by drip irrigation showed good effects on corn growth and resistance.

[0042] The above shows and describes the basic principles, main features and advantages of the present invention. Technical personnel in this industry should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only preferred examples of the present invention and are not used to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A method for promoting corn growth and improving plant resistance to corn leaf blight, characterized in that: The specific steps include: S1. The experimental field was divided into four plots, each plot was divided into 4 ridges, the ridge width was 0.61m, the ridge length was 15m, the aisle between each plot was 2m, and the protection rows in front and behind the experimental area were 10m each; S2, the first area is a blank control area where corn plants are planted normally (Control), the second area is a corn planted with Beauveria bassiana under drip irrigation (Bb), the third area is a corn planted with Euphorbia turcica (Et), and the fourth area is a corn planted with Beauveria bassiana after Euphorbia turcica (Bb+Et); S3. Record the plant height, stem diameter, and chlorophyll of corn at each growth stage, and record the dry and wet weight of the aboveground and underground parts at the harvest stage. Take leaf samples during the main disease occurrence period, and use the qPCR method to detect the pathogen content in the leaves; S4. Determine the grain yield. Take 10 corn plants from each plot, harvest corn ears, weigh them after killing and drying, and analyze the yield components such as thousand-grain weight and grain ear weight; S5. Compare all the data comprehensively to finally determine whether biocontrol microorganisms can promote corn growth and improve the plant's resistance to southern corn leaf blight.

2. The use according to claim 1, characterized in that: The corn plants are artificially inoculated with southern corn leaf blight at the trumpet stage.

3. The use according to claim 1, characterized in that: The dosage of the high spore pollen preparation per plot is 30 g (50 mL of 10^8 spores / mL inoculant is used for each plant, and the content of Beauveria bassiana in the inoculant is 50010^8 spores / g). The Beauveria bassiana preparation is evenly mixed with clean water, added to a small drip irrigation tank (volume 25-30 L), and irrigated using drip irrigation facilities.

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