Test strip for identifying gram-negative bacteria in peritoneal dialysis solution and preparation method thereof

By using polydopamine-coated colloidal gold-coupled antibiotic probes and detection lines formed by hydrogel coated with antibiotics in the peritoneal dialysate, the non-antibody-dependent side-flow immunochromatography test strips designed to form detection lines formed by hydrogels coated with antibiotics, the problems of low detection sensitivity, long time and high cost in the prior art are solved, and a fast and accurate detection effect is achieved.

CN119643855AInactive Publication Date: 2025-03-18NINGBO INST OF MATERIALS TECH & ENG CHINESE ACAD OF SCI +2
5 Cites 1 Cited by

Patent Information

Application Number
CN202510179526.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-02-19
Publication Date
2025-03-18
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

The prior art has problems of low sensitivity, time-consuming and high cost when detecting Gram-negative bacteria in peritoneal dialysate, especially in laboratories or clinical environments with limited resources, making it difficult to achieve rapid and accurate diagnosis.

Method used

Using polydopamine-coated colloidal gold-coupled antibiotic probes and detection lines formed by hydrogels coated with antibiotics, a non-antibody-dependent lateral flow immunochromatography test strip was designed to accurately identify Gram-negative bacteria in peritoneal dialysate within 10 minutes.

Benefits of technology

It realizes rapid and accurate detection of Gram-negative bacteria in peritoneal dialysate within 10 minutes, and the sensitivity is 1-2 orders of magnitude higher than that of traditional colloidal gold test strips, reducing the cost of the test strips and simplifying the preparation process.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention provides a test strip for identifying gram-negative bacteria in a peritoneal dialysis solution and a preparation method, and relates to the technical field of test strips. The test strip comprises a bottom plate as well as a sample pad, a combination pad, a coating pad and a water absorption pad which are sequentially arranged on the bottom plate, wherein a polydopamine coated colloidal gold coupled antibiotic probe is sprayed on the combination pad; a detection line is arranged on the coating pad, and the detection line is formed by hydrogel coated with antibiotics. According to the non-antibody-dependent immunochromatography test paper for detecting the gram-negative bacteria in the peritoneal dialysis solution, provided by the invention, a marker is simple and convenient to prepare, the use of paired antibodies is avoided, and the cost of the test paper is further reduced; moreover, time is gained for clinical diagnosis of microbial pathogens in dialysate, and correspondingly, antibiotic treatment is carried out on a patient in time. Each detection process can be completed within 10 min, the detection result is accurate, and the gram-negative bacteria in a peritoneal dialysis solution sample can be rapidly detected.
Need to check novelty before this filing date? Find Prior Art