Application of nano-hydroxyapatite in relieving abiotic stress of microplastics on plants

Abstract

The invention discloses an application of nano-hydroxyapatite in relieving abiotic stress of micro-plastics on plants. And adding nano-hydroxyapatite into the sodium citrate solution to form a suspension, and carrying out ultrasonic treatment, centrifugation and drying to obtain the sodium citrate modified nano-hydroxyapatite. In the soil containing the micro-plastics, after the plants are sown and emerged or transplanted, the sodium citrate modified nano-hydroxyapatite is added into the soil, so that the abiotic stress of the micro-plastics on the plants can be relieved. According to the invention, sodium citrate is utilized to modify nano-hydroxyapatite so as to relieve abiotic stress of MP generated after degradation of the PBAT mulching film on plants. The slow release and efficient utilization of P can be realized, the soluble sugar content, the soluble protein content and the VC content of the pepper can be increased under MP stress, the flowering period and the fruiting period of the pepper are advanced, the flowering number, the fruiting number and the plant height of the pepper are increased, and the malondialdehyde content of the pepper is reduced.

Description

Technical Field

[0001] The invention relates to the technical field of plant stress resistance, and in particular to the application of nano-hydroxyapatite in alleviating abiotic stress of microplastics on plants. Background Art

[0002] In 2004, Richard C. Thompson proposed the term "microplastics", which usually exist in the form of fragments, fibers or films, and are generally less than 5mm in size. As a new pollutant, the environmental problems brought about by microplastics have become a research hotspot. After microplastics enter the soil, they will not only change the physical and chemical properties of the soil (soil pH, bulk density, water storage capacity, nutrients, etc.), but also reduce the activity and functional diversity of soil microorganisms. These effects can directly affect the health and performance of plants by changing the root characteristics, nutrient absorption process and growth conditions of plants, causing serious white pollution. In terrestrial ecosystems, especially agricultural soils, it is a long-term storage reservoir for microplastics (MP). MP infiltrates agricultural soils from different sources, including landfills, sewage irrigation, application of biosolids and compost, polymer-based fertilizers and pesticides, and atmospheric deposition. Agricultural mulch is also a type of application that releases a large amount of micro- and nanoplastics (MNPs). In order to reduce and prevent residual pollution of mulch, with the development of biodegradable materials, biodegradable mulch came into being and was gradually applied to agricultural production as an ideal measure to solve the global "white pollution". For example, polybutylene terephthalate (PBAT) is widely used as a raw material for biodegradable mulch due to its excellent softness and ductility. It is one of the most common and widely used biodegradable materials on the market. At present, studies have shown that the impact of biodegradable plastics on plants may be much more serious than low-density polyethylene (LDPE). Wang reported (Wang F, Zhang X, Zhang S, et al. Interactions of microplasticsand cadmium on plant growth and arbuscular mycorrhizal fungal communities inan agricultural soil[J]. Chemosphere, 2020, 254: 126791) that 10% polylactic acid (PLA) significantly inhibited photosynthesis and reduced corn biomass; in addition, 10% 3-hydroxybutyric acid-co-3-hydroxyvalerate (PHBV) caused wheat plant death after 25 days.Qi et al. (Qi Y, Yang X, Pelaez AM, et al. Macro-and micro-plastics in soil-plant system: effects of plastic mulch film residues on wheat (Triticum aestivum) growth[J]. Science of the Total Environment, 2018,645: 1048-1056) found that compared with low-density polyethylene microplastics, starch-based biodegradable microplastics had stronger negative effects on wheat, including damage to leaf area, inhibition of plant height, delayed tillering, reduced fruiting rate, and reduced biomass and yield. Lian Yuhang et al. (Lian Yuhang, Liu Weitao, Shi Ruiying, et al. Effects of polyethylene and polylactic acid microplastics on soybean growth, physiology, biochemistry and metabolism[J]. Chinese Environmental Science, 2022, 42(6): 2894-2903.) PLA-MPs can significantly inhibit soybean root length and affect the antioxidant system of soybean leaves. In addition, it can also lead to the downregulation of organic acid metabolism pathways and carbohydrate metabolism pathways in leaves; Boots et al. (Boots B, Russell CW, Green D S. Effects ofmicroplastics in soil ecosystems: above and below ground[J]. Environmentalscience&technology, 2019, 53(19): 11496-11506) showed that PLA-MPs significantly inhibited the germination rate and stem length of ryegrass, while high-density polyethylene microplastics had little effect on it; deSouza Machado et al. (de SouzaMachado AA, Lau CW, Kloas W, et al. Microplastics can change soilproperties and affect plant performance[J]. Environmental science&technology,2019, 53(10): 6044-6052) The study found that MPs can have a significant impact on the biomass, root characteristics, elemental composition of tissues and soil microbial activity of green onions. After biodegradable plastics flow into the soil, they can be completely decomposed in about 100 days, leading to the production and accumulation of MPs, and may produce more biodegradable microplastics in the short term, posing a hidden danger to plant and soil health. In order to mitigate the toxic effects of microplastics on crops, Professor Yao Xiaoqin's team (Geng Z, Zhao B, DuanY, et al. Phosphorus mitigates the adverse effects of microplastics pollution on wheat and maize: Impacts on growth, photosynthesis, and antioxidantdefense[J]. Environmental and Experimental Botany, 2024, 228: 105993.) revealed the possible role of superphosphate in improving crop tolerance to microplastic pollution. Experiments showed that superphosphate supply mitigated the impact of microplastic pollution by improving the activities of photosynthetic pigments, peroxidase (POD) and polyphenol oxidase (PPO) in wheat and maize, and also improved the AsA-GSH cycle by improving the antioxidant enzyme activity in wheat and the non-enzymatic antioxidant content in maize, thereby reducing the impact of microplastic pollution. However, the phosphate fertilizer used cannot be slow-released, so its mitigation effect on abiotic stress caused by microplastics is limited, and these phosphate fertilizers are partially oxidized and fixed by iron, aluminum, etc. in the soil after entering the soil, and efficient utilization of phosphorus cannot be achieved. Therefore, a more effective method is needed to alleviate the abiotic stress of plants caused by mulch-derived microplastics. Summary of the invention

[0003] In view of the above-mentioned prior art, the purpose of the present invention is to provide an application of nano-hydroxyapatite in alleviating the abiotic stress of microplastics on plants. The present invention uses sodium citrate to modify nano-hydroxyapatite to alleviate the abiotic stress of MP produced after the degradation of PBAT mulch on plants. Not only can the slow release and efficient utilization of P be achieved, but also the soluble sugar content, soluble protein content, and VC content of peppers can be increased under MP stress, the flowering and fruiting period of peppers can be advanced, the number of flowers, the number of fruits, and the plant height of peppers can be increased, and the malondialdehyde content of peppers can be reduced.

[0004] To achieve the above object, the present invention adopts the following technical solution: The present invention provides an application of nano-hydroxyapatite in alleviating abiotic stress of microplastics on plants. In soil containing microplastics, after plants are sown, seedlings emerge or transplanted, nano-hydroxyapatite is added to the soil to alleviate the abiotic stress of microplastics on plants.

[0005] Preferably, the microplastics are microplastics derived from ground film; the ground film source comes from biodegradable plastic ground film.

[0006] More preferably, the material of the biodegradable plastic mulch is PBAT.

[0007] Preferably, the nano-hydroxyapatite is added to the 5-10 cm soil layer of the soil.

[0008] Preferably, the nano-hydroxyapatite is sodium citrate-modified nano-hydroxyapatite; the preparation method of the sodium citrate-modified nano-hydroxyapatite is: Nano-hydroxyapatite is added into a sodium citrate solution to form a suspension, and the suspension is subjected to ultrasonic treatment, centrifuged and dried to obtain sodium citrate-modified nano-hydroxyapatite.

[0009] More preferably, the mass ratio of sodium citrate to nano-hydroxyapatite is 1-2:5; the ultrasound is performed at room temperature for 12 hours, and the frequency of the ultrasound is 40 kHz.

[0010] Preferably, the plant is pepper.

[0011] Preferably, the amount of the nano-hydroxyapatite added to the soil is 20-100 mg P / kg in terms of phosphorus application.

[0012] More preferably, the amount of the nano-hydroxyapatite added to the soil is 50 mg P / kg in terms of phosphorus application.

[0013] Preferably, the application includes: increasing the soluble sugar content of pepper fruits, increasing the soluble protein content of pepper fruits, increasing the VC content of pepper fruits, reducing the malondialdehyde content of pepper leaves, advancing the flowering and fruiting periods of peppers, and increasing the number of flowers, the number of fruits and the plant height of peppers.

[0014] Beneficial effects of the present invention: (1) The present invention modifies nanohydroxyapatite with sodium citrate, effectively avoiding the aggregation of nanohydroxyapatite and improving its ability to slowly release P. The P contained in the nanohydroxyapatite is not easily oxidized and fixed by iron, aluminum, etc. in the soil. The biocompatibility and low toxicity of nanohydroxyapatite and the natural presence of its degradation products (i.e., phosphate and calcium ions) in the soil make it an alternative phosphate fertilizer, thereby improving the utilization rate of phosphate fertilizer.

[0015] (2) The present invention utilizes sodium citrate to modify nano-hydroxyapatite to effectively alleviate the abiotic stress of MP produced after the degradation of PBAT mulch film on plants, improve the quality of plant fruits, and effectively promote the flowering and fruiting dynamics of plants. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 : Flowering kinetics of pepper in each treatment group; Figure 2 : Kinetic diagram of pepper results in each treatment group; Figure 3: Plant height diagram of pepper in each treatment group; Figure 4 : Slow release diagram of phosphorus under three treatments. DETAILED DESCRIPTION

[0017] It should be noted that the following detailed descriptions are illustrative and are intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present application belongs.

[0018] As introduced in the background technology section, the supply of phosphorus improves the photosynthetic pigments, peroxidase (POD) and polyphenol oxidase (PPO) activities of wheat and corn, and also improves the AsA-GSH cycle by improving the antioxidant enzyme activity in wheat and the non-enzymatic antioxidant content in corn, thereby reducing the impact of microplastic pollution. However, the addition of conventional phosphorus fertilizers such as superphosphate cannot achieve effective controlled release of phosphorus, and these phosphorus fertilizers are partially oxidized and fixed by iron, aluminum, etc. in the soil after entering the soil, and cannot achieve efficient utilization of phosphorus.

[0019] Based on this, the purpose of the present invention is to provide the application of nano-hydroxyapatite in alleviating the abiotic stress of microplastics on plants. The present invention adds sodium citrate-modified nano-hydroxyapatite to the soil, which can effectively alleviate the abiotic stress of MP produced by the degradation of PBAT mulch on plants. Sodium citrate has mild acidity, can stimulate the growth of beneficial microorganisms in the soil, promote the activity of soil enzymes, and improve soil texture. Modifying nano-hydroxyapatite with sodium citrate can avoid the agglomeration of nano-hydroxyapatite and further improve the sustained release of P. However, it is necessary to control the amount of sodium citrate added, and the mass ratio of sodium citrate to nano-hydroxyapatite is 1~2:5. The effect is best. In addition, the sodium citrate-modified nano-hydroxyapatite does not alleviate the abiotic stress of MP on plants. The optimal concentration in the soil is 50mg P / kg.

[0020] In order to enable those skilled in the art to more clearly understand the technical solution of the present application, the technical solution of the present application will be described in detail below in conjunction with specific embodiments.

[0021] The test materials used in the examples of the present invention are all conventional test materials in the art and can be purchased through commercial channels.

[0022] Example 1 0.15 g of sodium citrate was dissolved in 20 mL of distilled water to obtain a sodium citrate solution, and 0.5 g of nano-hydroxyapatite was added to the sodium citrate solution to form a nano-suspension; the ultrasonic frequency was adjusted to 40 KHZ, and ultrasonication was performed at 25° C. for 12 h; the powder material was centrifuged and dried in a vacuum drying oven at 60° C. for 24 h to obtain sodium citrate-modified nano-hydroxyapatite.

[0023] Comparative Example 1 The difference from Example 1 is that 0.05 g of sodium citrate is added.

[0024] Comparative Example 2 The difference from Example 1 is that 0.5 g of sodium citrate is added.

[0025] Test Example 1 The experiment was divided into 10 groups: normal group, blank group, superphosphate group, nanohydroxyapatite group, sodium citrate group, example group (example groups 2 to 4), and comparative group (comparative example groups 1 to 2). Except for the normal group, the other groups added PBAT microplastics to the potted soil and transplanted pepper seedlings to simulate the abiotic stress of MP on pepper.

[0026] Among them, the normal group used soil without PBAT microplastics and did not add any phosphate fertilizer; the blank group used soil containing PBAT microplastics and did not add any phosphate fertilizer; the superphosphate group used soil containing PBAT microplastics and added superphosphate at a concentration of 50 mg P / kg; the nanohydroxyapatite group used soil containing PBAT microplastics and added nanohydroxyapatite at a concentration of 50 mg P / kg; the sodium citrate group used soil containing PBAT microplastics and added sodium citrate at the same concentration as that of the sodium citrate contained in the Example 3 group; the Example 2 group used soil containing PBAT microplastics and added sodium citrate-modified nanohydroxyapatite prepared in Example 1 at a concentration of 20 mg P / kg; the Example 3 group used soil containing PBAT microplastics and added sodium citrate-modified nanohydroxyapatite prepared in Example 1 at a concentration of 50 mg P / kg; the Example 4 group used soil containing PBAT microplastics and added sodium citrate-modified nanohydroxyapatite prepared in Example 1 at a concentration of 100 mgP / kg; Comparative Example 1 group used soil containing PBAT microplastics, and added sodium citrate-modified nano-hydroxyapatite prepared in Comparative Example 1 at a concentration of 50 mg P / kg; Comparative Example 2 group used soil containing PBAT microplastics, and added sodium citrate-modified nano-hydroxyapatite prepared in Comparative Example 2 at a concentration of 50 mg P / kg.

[0027] The test soil was screened to ensure that it did not contain microplastics, and 0.8 kg (dry weight) of the test soil was placed in the pots of the normal group. Another 9 groups of 0.8 kg (dry weight) of the test soil after screening were taken, PBAT microplastics (concentration of 1% w / w, particle size of 30-65 μm) were added thereto, and phosphate fertilizer or sodium citrate was added according to the settings of the above groups, and they were placed in pots, and recorded as blank group, superphosphate group, nanohydroxyapatite group, sodium citrate group, example group (example group 2-4), and comparative example group (comparative example group 1-2).

[0028] Before formal cultivation, the pepper seeds (sweet pepper seeds numbered CJ20 provided by Sichuan Agricultural University) were soaked in 55°C hot water for 15 minutes for disinfection and sterilization, then taken out and rinsed repeatedly with deionized water, and soaked in warm water for 2 hours to allow the seeds to absorb enough water. Finally, the seeds were taken out and neatly placed in a seedling pot and placed in a constant temperature incubator at 28°C for seedling cultivation. When the seedlings grew to five leaves and one heart, pepper seedlings with consistent growth were selected and one plant was transplanted into the pot of each treatment. Each treatment was repeated 4 times.

[0029] At the same time, nitrogen fertilizer (ammonium sulfate) and potassium fertilizer (potassium sulfate) were used as base fertilizer in the above 10 groups, and the addition amount was 40 mg / kg. The fertilization method was broadcasting, and nitrogen fertilizer and potassium fertilizer were broadcasted and top-dressed. A total of 2 topdressings were applied at the initial flowering stage and the initial fruiting stage, and the topdressing amount was 35 mg / kg. The soil moisture content during the growth period was controlled at about 70% of the field water holding capacity. The pots were moved every two days during the planting period to ensure that no weeds and pests occurred during the experiment.

[0030] During the planting period, the growth of the plants was analyzed. When the peppers entered the budding stage, three plants with medium and relatively consistent growth were selected from each treatment to measure the plant height and leaf SPAD value every 7 days until the last yield measurement. After 60 days, the peppers matured and were harvested; the growth indicators of each group of plants (fresh weight and dry weight of each part of the plant, number of flowers and fruits, root morphology, stem height and stem thickness) were recorded, and the soluble sugar, soluble protein, VC content of the pepper fruits and the malondialdehyde of the pepper leaves were measured. The results are shown in Figures 1 to 3 and Tables 2~3.

[0031] Soluble sugar was determined using the anthrone colorimetric method: Extraction of soluble sugars from samples: Weigh 0.5-1.0 g of the chopped and mixed pepper fruits from each group, put them into a large test tube, add 15 mL of distilled water, boil them in a boiling water bath for 20 min, take them out and cool them, filter them into a 100 mL volumetric flask, rinse the residue several times with distilled water, and make up to the mark.

[0032] Sample determination: Take 1.0 mL of the sample extract to be tested and add 5 mL of anthrone reagent (0.2 g of anthrone, dissolved in 100 mL of concentrated acid, prepared and used on the same day), and perform color development and determine the optical density in the same manner as above. Repeat 3 times.

[0033] Calculation results: Soluble sugar content (%) = amount of sugar obtained from the standard curve (μg) × volume of extract (mL) × dilution factor / (volume of sample solution for determination (ml) × sample weight (g) × 10 6)×100%.

[0034] The soluble protein content was determined by Coomassie Brilliant Blue G-250 staining: 1. Reagents and preparation Extract (50mmol·L -1 Tris-HCl, pH 7.8, containing 0.5 mmol L -1 MgCl2, 1 mmol·L - 1 EDTA,).

[0035] Preparation of Coomassie Brilliant Blue Solution: Weigh 100 mg of Coomassie Brilliant Blue G-250, add 50 mL of 95% ethanol and 100 mL of 85% phosphoric acid, and then dilute to 1000 mL. 0.15 mol·L -1 NaCl solution.

[0036] Preparation of bovine serum albumin standard stock solution: Use 0.15 mol·L -1 NaCl solution was prepared to 100 μg ml -1 Bovine serum albumin standard solution.

[0037] 2. Preparation of bovine serum albumin standard curve Take 6 test tubes, number them, and prepare bovine serum albumin standard solution with a content of 0-100 μg in each tube according to Table 1.

[0038] Table 1 After adding the reagents in Table 1, shake well, use tube 0 as the blank control, and measure its absorbance (A) value at a wavelength of 595nm.

[0039] Standard curve drawing: draw the standard curve with bovine serum albumin content as the horizontal axis and absorbance value as the vertical axis.

[0040] 3. Protein extraction Weigh 0.3-0.5 g of pepper fruit obtained from each group, cut it into pieces in a frozen mortar, add 3 mL of extract, add a small amount of quartz sand, and quickly grind it into a homogenate in an ice bath. Pour the homogenate into a centrifuge tube, and then wash the homogenate in the mortar into a centrifuge tube with 5 mL of extract (twice), and then centrifuge it at 10,000 r / min, 4°C for 20 min. The supernatant is the soluble protein extract.

[0041] 4. Determination Take 0.1 mL of the above supernatant and put it into 2 test tubes respectively, add 0.9 mL of Tris buffer to each tube, add 1 mL of Tris buffer to the blank control tube, and then add 5 mL of Coomassie Brilliant Blue staining solution to each tube, shake well, and measure the absorbance (A) value at a wavelength of 595 nm.

[0042] 5. Result calculation According to the measured absorbance value of the sample solution, the protein content is found from the standard curve, and the soluble protein content is calculated according to the following formula: .

[0043] The Vc content was determined by the molybdenum blue colorimetric method (Li Jun, "Food Science", 2000).

[0044] The malondialdehyde content was tested according to the following method: Experimental medicine: 1. 10% trichloroacetic acid (TCA); 2. 0.5% thiobarbituric acid (dissolved in 10% trichloroacetic acid); 3. Quartz sand.

[0045] Experimental methods: 1. Take 1-2 chili leaves, wash and dry them, cut them into 0.5 cm long pieces, and mix them well.

[0046] 2. Weigh 0.3 g of the cut leaves, put them into a mortar in an ice bath, add 2 mL of 10% TCA and a small amount of quartz sand, and grind them into a homogenous slurry.

[0047] 3. Then centrifuge at 4°C, 12,000 ppm for 15 min and collect the supernatant.

[0048] 4. Take 1.5 mL of the supernatant from the centrifugation (add 1.5 mL of 10% TCA for the control), add the same volume of 0.5% TBA solution, react the mixture in a boiling water bath for 30 min, cool it quickly and then centrifuge it again.

[0049] 5. Take the supernatant and measure the absorbance at wavelengths of 532nm, 450nm and 600nm.

[0050] Calculated content: The content of MDA in the sample was calculated based on the weight of the plant tissue: MDA concentration (μmoL / L) = 6.45·(OD 532 -OD 600 )-0.56·OD 450 .

[0051] Table 2 Root morphology Table 3 Physiological indicators according to Figures 1 to 3 It can be seen that under the stress of mulch-derived microplastics PBAT, compared with conventional phosphate fertilizers, sodium citrate and nanohydroxyapatite, the application of sodium citrate-modified nanohydroxyapatite can advance the flowering period and increase the number of flowers; it can also advance fruiting and increase the number of fruits; it can also increase the height of the plant. This shows that sodium citrate-modified nanohydroxyapatite can improve the flowering and fruiting dynamics under the stress of microplastics PBAT.

[0052] It can be seen from Table 2 that the peppers in Example 3 group have more developed root systems, which is beneficial to nutrient absorption and plant growth.

[0053] According to Table 3, under PBAT stress, when the phosphorus concentration was 50 P / kg, the pepper plants with sodium citrate-modified nanohydroxyapatite had the highest soluble sugar content, soluble protein content, and VC content, while the malondialdehyde content was the lowest. This indicates that sodium citrate-modified nanohydroxyapatite can improve the quality of peppers under microplastic PBAT stress.

[0054] Test Example 2 The experiment was divided into 3 groups: superphosphate group, nano-hydroxyapatite group and Example 3 group, and the specific settings were the same as those of Experimental Example 1.

[0055] The release of phosphate in the superphosphate group, nanohydroxyapatite group and Example 3 group was analyzed in aqueous solution. The above three groups of phosphate fertilizers were added to a sealed glass bottle containing deionized water so that the concentration was 200 ppm P, and placed on a constant temperature oscillator controlled at 25°C. Samples were taken at corresponding times (1, 3, 5, 10, 20, 30, 40, 50, 60, 90, 120, 180 min). The concentration of phosphate was determined by a UV-Vis spectrometer (Thermo Scientific, GENESYS50, USA), and the concentration of phosphate was recorded by the molybdenum blue method at 880 nm using a UV-Vis spectrometer. The P release of the above three groups is shown in Figure 4 .

[0056] according to Figure 4 It can be seen that after the superphosphate group came into contact with water, all the P was released. However, the P in the nanohydroxyapatite group and the Example 3 group was slowly released, but the P release amount in the Example 3 was higher, which could meet the P demand of the pepper during its growth.

[0057] The above description is only the preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. Application of nano-hydroxyapatite in alleviating abiotic stress of microplastics on plants, characterized in that: In soil containing microplastics, after plants are sown or transplanted, nanohydroxyapatite is added to the soil to alleviate the abiotic stress of microplastics on plants.

2. The use according to claim 1, characterized in that: The microplastics are microplastics from ground film sources; the ground film sources come from biodegradable plastic ground films.

3. The use according to claim 2, characterized in that: The material of the biodegradable plastic mulch is PBAT.

4. The use according to claim 1, characterized in that: The nano hydroxyapatite is added into the 5-10 cm soil layer of the soil.

5. The use according to claim 1, characterized in that: The nano-hydroxyapatite is sodium citrate-modified nano-hydroxyapatite; the preparation method of the sodium citrate-modified nano-hydroxyapatite is: Nano-hydroxyapatite is added into a sodium citrate solution to form a suspension, and the suspension is subjected to ultrasonic treatment, centrifuged and dried to obtain sodium citrate-modified nano-hydroxyapatite.

6. The use according to claim 5, characterized in that: The mass ratio of sodium citrate to nano-hydroxyapatite is 1-2:5; the ultrasound is performed at room temperature for 12 hours, and the frequency of the ultrasound is 40 kHz.

7. The use according to claim 1, characterized in that: The plant is pepper.

8. The use according to claim 7, characterized in that: The amount of the nano-hydroxyapatite added to the soil is 20-100 mg P / kg based on the amount of phosphorus applied.

9. The use according to claim 8, characterized in that: The amount of the nano-hydroxyapatite added to the soil is 50 mg P / kg, calculated as the amount of phosphorus applied.

10. The use according to claim 7, characterized in that: The applications include: increasing the soluble sugar content of pepper fruits, increasing the soluble protein content of pepper fruits, increasing the VC content of pepper fruits, reducing the malondialdehyde content of pepper leaves, advancing the flowering and fruiting periods of peppers, and increasing the number of flowers, the number of fruits and the plant height of peppers.

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