A kind of winter cabbage fungicide and preparation method thereof

Abstract

The present invention belongs to the field of botanical fungicide preparation, and particularly relates to a winter cabbage fungicide and its preparation method. A porous matrix prepared through an esterification and cross-linking reaction is fully adsorbed with a potassium hydroxide solution to obtain a high-efficiency bactericidal nanoparticle mixture with a viscous solution and a bactericidal and antiviral natural extract obtained through extraction and concentration. The resulting high-efficiency bactericidal suspension can effectively prevent and control winter cabbage diseases. A freeze-dried disease-preventing and resistance-enhancing powder is mixed into the breathable emulsion formed by mixing and emulsifying polydimethylsiloxane and propylene glycol. The resulting disease-preventing and resistance-enhancing composition can maintain the disease resistance of winter cabbage for a long time. The plant growth-promoting mixture is evenly mixed with the high-efficiency bactericidal component. The resulting winter cabbage fungicide effectively prevents and controls diseases caused by pathogenic microorganisms, enhances the plant's own disease resistance, and is biocompatible without disrupting ecological balance.

Description

Technical Field

[0001] The invention belongs to the field of preparation of plant-derived fungicides, and particularly relates to a winter cabbage fungicide and a preparation method thereof. Background Art

[0002] Cabbage, as a vegetable with strong cold resistance, is an important edible crop in winter. It can grow normally at lower temperatures and can withstand temperatures as low as -3°C. The best time to plant winter cabbage is usually in autumn. Early-maturing varieties are sown in early to mid-August, medium- and late-maturing varieties are sown in late August to early September, and late-maturing varieties are sown in late August. Winter cabbage is rich in nutrients such as vitamin C, vitamin E, iron, potassium, vitamin A, etc. It is also rich in bioactive phytochemicals such as glucosinolates, which have potential benefits such as antioxidant, antibacterial, and immune regulation.

[0003] However, the cold climate and humidity changes in winter also make cabbage susceptible to a variety of diseases. Downy mildew is one of the most common diseases of cabbage in winter. It is mainly manifested by the appearance of a white mold layer on the back of the leaves. In severe cases, the leaves turn yellow and fall off. Soft rot mainly invades the cabbage through wounds, causing the tissue to soften and rot. Black spot disease mainly harms the leaves of cabbage, forming black spots and affecting photosynthesis. Viral disease is a major problem in cabbage production, which can cause symptoms such as mottled and wrinkled leaves. Cabbage dry heart disease begins to occur in the rosette stage, and young leaves have dry edges. The symptoms of the disease are more obvious in the heading stage, and the disease is mostly concentrated in the middle of the leaf head. The upper part of the leaf gradually becomes dry and yellow, the mesophyll becomes dry and papery, and the veins are yellow-brown to dark black.

[0004] Currently, the existing winter cabbage fungicide preparation technology has the following problems: First, the existing winter cabbage fungicides are not effective in preventing and controlling diseases caused by bacteria, fungi, and viruses, and the disease prevention and control effects are unstable. Most fungicides can only prevent and control diseases caused by a single microorganism. Second, the existing winter cabbage fungicides are only effective for a certain period of time and cannot prevent and control cabbage diseases in the long term, requiring long-term and large-scale application. Third, if the existing winter cabbage fungicides fall into the soil, they will cause soil acidification, have an adverse effect on beneficial microorganisms in the soil, and disrupt the ecological balance. Summary of the Invention

[0005] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a winter cabbage fungicide and a preparation method thereof. In order to solve the problems that the existing winter cabbage fungicides have an insignificant effect on preventing and controlling diseases caused by pathogenic microorganisms, cannot prevent and control winter cabbage diseases for a long time, and destroy the ecological balance, the present invention uses a high-efficiency bactericidal suspension, a disease prevention and resistance-enhancing composition, and a plant growth-promoting mixture to mix. The prepared winter cabbage fungicide can effectively prevent and control diseases caused by pathogenic microorganisms, maintain a long-term bactericidal effect, has biocompatibility, and does not destroy the ecological balance.

[0006] In order to achieve the above object, the technical solution adopted by the present invention is as follows: the present invention provides a winter cabbage fungicide, and the raw materials for preparing the winter cabbage fungicide specifically include the following components in parts by weight:

[0007] 70-90 parts of high-efficiency bactericidal suspension, 30-40 parts of disease prevention and resistance enhancement composition, 4-8 parts of methionine, 3-7 parts of methyl gallate, 6-9 parts of p-chlorophenoxyacetic acid, and 2-5 parts of allantoin.

[0008] Preferably, the raw materials for preparing the high-efficiency bactericidal suspension include the following components in parts by weight: 11-13 parts of Herba Euphorbiae Ichthyophthirius, 7-10 parts of Rhizoma Cyrthospermi, 8-12 parts of Viola yedoensis, 4-7 parts of Phyllanthus urinaria, 9-13 parts of potassium diformate, 26-33 parts of citrus fiber powder, 11-14 parts of citric acid, 1.8-2.6 parts of trisodium citrate, 8-12 parts of potassium hydroxide, and 12-16 parts of potassium alginate.

[0009] Preferably, the raw materials for preparing the disease prevention and resistance enhancement composition include the following components in parts by weight: 2-4 parts of salicylic acid, 3-5 parts of magnesium chloride, 3-6 parts of α-mannosidase, 17-24 parts of polydimethylsiloxane, 8-11 parts of propylene glycol, 25-31 parts of oxidized starch, and 13-16 parts of glutaraldehyde.

[0010] Preferably, the method for preparing the highly effective bactericidal suspension comprises the following steps:

[0011] S1. Put Herba Euphorbiae Herba, Rhizoma Cyrthospermi, Viola yedoensis, and Herba Phyllanthiphyllae into a grinder with a power of 1.5-1.8 kW, a grinding temperature of 23-28°C, a grinding time of 15-20 min, and a grinding speed of 3500-4500 r / min. After grinding, put them into an ultrasonic extractor with a power of 1.3-1.6 kW with a 50% ethanol solution, an extraction pressure of 1.5 MPa, an extraction frequency of 25 kHz, an extraction temperature of 40-45°C, and an extraction time of 8-13 min. After extraction, put them into a herbal concentrator with a power of 2.5-3.5 kW, a concentration temperature of 40-60°C, a concentration time of 35-45 min, and a concentration speed of 10-20 r / min to obtain a bactericidal and antiviral natural extract;

[0012] S2. Place potassium diformate and ultrapure water in a stirring tank with a power of 1.5-1.8 kW, stir at a temperature of 25-30° C., stir for 8-12 min, and stir at a speed of 150-180 r / min. After complete dissolution, obtain a potassium diformate solution;

[0013] S3, placing the citrus fiber powder, the potassium diformate solution prepared in S2, citric acid, trisodium citrate, and N-methylpyrrolidone into a reactor with a power of 2.2-2.5 kW, reacting at a temperature of 90-100° C., for a reaction time of 1.3-1.6 h, and a reaction speed of 200-300 r / min, to carry out an esterification and cross-linking reaction to obtain a porous matrix;

[0014] S4, placing potassium hydroxide and ultrapure water in a magnetic stirrer with a power of 1.6-1.8 kW, stirring at a temperature of 28-33°C, stirring for 10-15 min, and stirring at a speed of 320-350 r / min, adding the porous matrix prepared in S3 after dissolution, stirring at a temperature of 25-30°C, stirring for 20-25 min, and stirring at a speed of 400-600 r / min, stirring for sufficient adsorption, placing in a vacuum freeze nano spray dryer with a power of 2.3-2.8 kW, the vacuum degree is -0.05 MPa, the freeze nano spray drying temperature is -40°C, the freeze nano spray drying particle size is 100 nm, the freeze nano spray drying time is 2.5-3h, and freeze spray drying is performed to obtain highly efficient bactericidal nanoparticles;

[0015] S5. Place potassium alginate and ultrapure water in a stirring tank with a power of 1.5-1.8 kW, stir at a temperature of 60-80° C., stir for 10-15 min, and stir at a speed of 200-240 r / min to obtain a viscous solution.

[0016] S6. Add the bactericidal and antiviral natural extract prepared in S1 and the highly effective bactericidal nanoparticles prepared in S4 into the viscous solution prepared in S5, stir at a temperature of 30-36°C, for 13-18 min, at a stirring speed of 260-280 r / min, and mix to obtain a highly effective bactericidal suspension.

[0017] Furthermore, in S1, the solid-liquid ratio of the Herba Euphorbiae Ichthyophthirius and the 50% ethanol solution is 1.5:1 g / mL, and in S2, the mass fraction of the potassium diformate in ultrapure water is 35-39%.

[0018] Furthermore, in S3, the material-liquid ratio of the citrus fiber powder to N-methylpyrrolidone is 0.2:1 g / mL, in S4, the mass fraction of the potassium hydroxide in ultrapure water is 36-42%, and in S5, the mass fraction of the potassium alginate in ultrapure water is 2-4%.

[0019] Preferably, the method for preparing the disease prevention and resistance enhancement composition specifically comprises the following steps:

[0020] L1. Place salicylic acid, magnesium chloride, α-mannosidase, and 50% ethanol solution into a 1.2-1.5 kW stirrer at a stirring temperature of 23-28°C for 10-15 minutes at a stirring speed of 180-220 r / min, and mix and dissolve to obtain a disease resistance-enhancing preparation.

[0021] L2. Place polydimethylsiloxane and propylene glycol in a 2.5 kW high-speed stirrer, stir at a temperature of 25-30° C., a speed of 6000-8000 r / min, and a stirring time of 20-30 min, and emulsify them by high-speed stirring to obtain a breathable emulsion;

[0022] L3. Place oxidized starch, glutaraldehyde, and ultrapure water in a reactor with a power of 2.7-2.9 kW, react at a temperature of 50° C., a reaction speed of 200 r / min, and a reaction time of 2.1-2.3 h to obtain a microporous foam material;

[0023] L4, placing the microporous foam material prepared in L3 into the disease resistance enhancing preparation prepared in L1, stirring at a temperature of 23-28°C, a stirring time of 14-18 min, and a stirring speed of 230-250 r / min. After mixing, placing in a freeze dryer with a power of 1.6-2.2 kW, freeze drying at a pressure of 300 Pa and a reduced pressure drying temperature of -45°C, freeze drying for 12 h, heating to -25°C and freeze drying for 8 h, and then freeze drying at a temperature of 15°C for 5 h to obtain a disease prevention and resistance enhancing powder;

[0024] L5. Put the disease prevention and resistance-enhancing powder prepared in L4 into the breathable emulsion prepared in L2, put it into an ultrasonic stirrer with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min, and perform ultrasonic dispersion to obtain a disease prevention and resistance-enhancing composition.

[0025] Furthermore, in L1, the material-liquid ratio of salicylic acid to 50% ethanol solution is 0.15:1 g / mL.

[0026] Furthermore, in L3, the mass fraction of the glutaraldehyde in ultrapure water is 20-28%.

[0027] The present invention also provides a method for preparing a winter cabbage fungicide, which specifically comprises the following steps:

[0028] Step 1: Place methionine, methyl gallate, p-chlorophenoxyacetic acid, allantoin, and 70% ethanol solution into a stirring tank with a power of 1.5-1.8 kW, stir at a temperature of 70-80° C., stir for 14-19 minutes, and stir at a speed of 200-240 r / min to obtain a plant growth promoting mixture;

[0029] Step 2: Place the high-efficiency bactericidal suspension and the disease prevention and resistance-enhancing composition into a stirrer with a power of 1.8-2.1 kW, stir at a temperature of 30-38° C., stir for 20-30 minutes, and stir at a speed of 300-400 r / min to obtain a high-efficiency bactericidal component;

[0030] Step 3: Add the plant growth promoting mixture prepared in step 1 to the high-efficiency bactericidal component prepared in step 2, stir at a temperature of 25-35°C, stir for 18-26 min, stir at a speed of 300-400 r / min, mix evenly, and obtain a winter cabbage fungicide.

[0031] Preferably, in step 1, the material-liquid ratio of methionine to 70% ethanol solution is 0.13:1 g / mL.

[0032] The beneficial effects achieved by the present invention are as follows:

[0033] The invention adopts Herba Euphorbiae Herba, Cyperus rotundus, Viola yedoensis and Phyllanthus urinariae, crushes them and then extracts and concentrates them. The obtained bactericidal and antiviral natural extract contains isoquercetin, euphorbic acid, brucine, strychnine, disulfide, esculent acid, ferulic acid, phyllanthine and other compounds with broad-spectrum bactericidal, antiviral and biocompatible properties. Potassium diformate solution and citrus fiber powder are mixed with citric acid as a cross-linking agent. Under the catalytic action of trisodium citrate, the carboxyl groups on citric acid and potassium diformate react with the hydroxyl groups in the molecules of citrus fiber powder to form ester bonds, thereby forming a porous matrix with bactericidal, antiviral and adsorption effects. Potassium oxide cooperates with potassium diformate to destroy and penetrate the cell walls of bacteria and fungi through hydrolysis, change the permeability of bacterial and fungal cell membranes, and denature the proteins on the surface of viruses. After potassium alginate is dissolved in ultrapure water, the viscous solution prepared can adhere to the peptidoglycan of bacterial cell walls, the chitin of fungal cell walls, and the glycoprotein on the surface of viruses. The high-efficiency bactericidal nanoparticles that fully absorb the potassium hydroxide solution are mixed with the viscous solution and the bactericidal and antiviral natural extracts. The prepared high-efficiency bactericidal suspension can effectively kill parasitic downy mildew, carrot soft rot pectin bacteria, pineapple pantothecoides, and butyric acid bacteria that cause winter cabbage disease. Pseudomonas aeruginosa, tobacco mosaic virus and other pathogenic microorganisms, and does not harm the cabbage itself; salicylic acid, magnesium chloride and α-mannosidase are mixed to obtain a disease resistance-enhancing preparation that can regulate the activity of enzymes contained in winter cabbage, induce the production of reactive oxygen species, activate plant immune response, and enhance the disease resistance of winter cabbage itself; after polydimethylsiloxane and propylene glycol are mixed and emulsified, the breathable emulsion formed is air-dried to form a film that is breathable and can prevent plants from suffering from frost damage; the hydroxyl groups in the oxidized starch molecules and the aldehyde groups in the glutaraldehyde molecules undergo a condensation reaction, and the obtained microporous foaming material absorbs and enhances the disease resistance. The invention discloses a novel winter cabbage fungicide comprising a novel anti-disease and resistance-enhancing powder prepared by ultrasonically dispersing the powder in a breathable emulsion, and the obtained anti-disease and resistance-enhancing composition can enable winter cabbage to maintain disease resistance for a long time. The invention also discloses a winter cabbage fungicide prepared by mixing methionine, methyl gallate, p-chlorophenoxyacetic acid, and allantoin, and the mixture can promote plant rooting, enhance plant nutrient absorption, and increase plant growth rate and dry matter accumulation. The mixture is mixed with a high-efficiency bactericidal component to prepare a winter cabbage fungicide that effectively prevents and controls diseases caused by pathogenic microorganisms, does not harm plant leaves, maintains a long-term bactericidal effect, enhances the disease resistance of the plant itself, and has biocompatibility without destroying the ecological balance. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] In order to more clearly illustrate the present invention or the technical solutions in the prior art, the following will be described in a clear and easy-to-understand manner with reference to the accompanying drawings. Obviously, the drawings described below are only for the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0035] Figure 1 This is a scanning electron microscope image of the internal structure of the highly effective bactericidal nanoparticles described in Experimental Example 1 of the present invention;

[0036] Figure 2 This is a graph showing the effect of controlling downy mildew on Chinese cabbage described in Experimental Example 1 of the present invention;

[0037] Figure 3 This is a graph showing the effect of preventing and controlling soft rot of Chinese cabbage described in Experimental Example 1 of the present invention;

[0038] Figure 4 This is a graph showing the results of the prevention and treatment of Chinese cabbage mosaic disease described in Experimental Example 1 of the present invention;

[0039] Figure 5 This is a graph showing the soil pH measurement results described in Experimental Example 2 of the present invention;

[0040] Figure 6 This is a graph showing the results of determining the bacterial colony count in the soil described in Experimental Example 2 of the present invention. DETAILED DESCRIPTION

[0041] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0042] Unless otherwise defined, all technical and scientific terms used herein have the same meanings as those familiar to those skilled in the art. Furthermore, any methods and materials similar or equivalent to those described herein can be applied to the present invention. The preferred embodiments and materials described herein are for illustrative purposes only and are not intended to limit the scope of this application.

[0043] The experimental methods in the following examples, unless otherwise specified, are conventional methods; the test materials and test strains used in the following examples, unless otherwise specified, are purchased from commercial channels.

[0044] Example 1: This example provides a winter cabbage fungicide and a preparation method thereof. The winter cabbage fungicide comprises the following components in parts by weight:

[0045] 70 parts of high-efficiency bactericidal suspension, 30 parts of disease prevention and resistance enhancement composition, 4 parts of methionine, 3 parts of methyl gallate, 6 parts of p-chlorophenoxyacetic acid, and 2 parts of allantoin.

[0046] The raw materials for preparing the high-efficiency bactericidal suspension include the following components in parts by weight: 11 parts of Herba Euphorbiae Ichthyophthirius, 7 parts of Rhizoma Cyrthospermi, 8 parts of Viola yedoensis, 4 parts of Phyllanthus urinariae, 9 parts of potassium diformate, 26 parts of citrus fiber powder, 11 parts of citric acid, 1.8 parts of trisodium citrate, 8 parts of potassium hydroxide, and 12 parts of potassium alginate.

[0047] The raw materials for preparing the disease prevention and resistance enhancement composition include the following components in parts by weight: 2 parts of salicylic acid, 3 parts of magnesium chloride, 3 parts of α-mannosidase, 17 parts of polydimethylsiloxane, 8 parts of propylene glycol, 25 parts of oxidized starch, and 13 parts of glutaraldehyde.

[0048] The preparation method of the highly effective bactericidal suspension specifically comprises the following steps:

[0049] S1. Put Herba Euphorbiae Herba, Rhizoma Cyrthospermi, Viola yedoensis, and Phyllanthus urinariae into a 1.5 kW grinder, the grinding temperature is 23°C, the grinding time is 15 min, and the grinding speed is 3500 r / min. After grinding, put it into an ultrasonic extractor with a power of 1.3 kW and a 50% ethanol solution, the extraction pressure is 1.5 MPa, the extraction frequency is 25 kHz, the extraction temperature is 40°C, and the extraction time is 8 min. After extraction, put it into a herbal concentrator with a power of 2.5 kW, the concentration temperature is 40°C, the concentration time is 35 min, and the concentration speed is 10 r / min to obtain a bactericidal and antiviral natural extract;

[0050] S2. Potassium diformate and ultrapure water were placed in a stirring tank with a power of 1.5 kW, stirred at a temperature of 25°C, for 8 minutes, and at a stirring speed of 150 r / min. After complete dissolution, a potassium diformate solution was obtained;

[0051] S3, placing the citrus fiber powder, the potassium diformate solution prepared in S2, citric acid, trisodium citrate, and N-methylpyrrolidone into a 2.2 kW reactor, at a reaction temperature of 90°C, a reaction time of 1.3 h, and a reaction speed of 200 r / min, to carry out an esterification and cross-linking reaction to obtain a porous matrix;

[0052] S4, placing potassium hydroxide and ultrapure water in a magnetic stirrer with a power of 1.6 kW, stirring at a temperature of 28°C, stirring for 10 min, and stirring at a speed of 320 r / min, adding the porous matrix prepared in S3 after dissolution, stirring at a temperature of 25°C, stirring for 20 min, and stirring at a speed of 400 r / min, stirring for sufficient adsorption, placing in a vacuum freeze nano spray dryer with a power of 2.3 kW, the vacuum degree is -0.05 MPa, the freeze nano spray drying temperature is -40°C, the freeze nano spray drying particle size is 100 nm, the freeze nano spray drying time is 2.5 h, and freeze spray drying is performed to obtain highly efficient bactericidal nanoparticles;

[0053] S5. Place potassium alginate and ultrapure water in a 1.5 kW stirring tank at 60°C for 10 min at a stirring speed of 200 r / min and stir evenly to obtain a viscous solution.

[0054] S6. Add the bactericidal and antiviral natural extract prepared in S1 and the highly effective bactericidal nanoparticles prepared in S4 into the viscous solution prepared in S5, stir at a temperature of 30°C, a stirring time of 13 min, and a stirring speed of 260 r / min, and stir and mix to obtain a highly effective bactericidal suspension.

[0055] In S1, the solid-liquid ratio of the Herba Euphorbiae Ichthyophthirius and the 50% ethanol solution is 1.5:1 g / mL. In S2, the mass fraction of the potassium diformate in ultrapure water is 35%.

[0056] In S3, the material-liquid ratio of the citrus fiber powder to N-methylpyrrolidone is 0.2:1 g / mL, in S4, the mass fraction of the potassium hydroxide in ultrapure water is 36%, and in S5, the mass fraction of the potassium alginate in ultrapure water is 2%.

[0057] The method for preparing the disease prevention and resistance enhancement composition specifically comprises the following steps:

[0058] L1. Place salicylic acid, magnesium chloride, α-mannosidase, and 50% ethanol solution in a 1.2 kW stirrer at a stirring temperature of 23°C for 10 minutes at a stirring speed of 180 r / min, and mix and dissolve to obtain a disease resistance-enhancing preparation.

[0059] L2. Place polydimethylsiloxane and propylene glycol in a 2.5 kW high-speed stirrer at a high-speed stirring temperature of 25° C., a high-speed stirring speed of 6000 r / min, and a high-speed stirring time of 20 min, and emulsify them under high-speed stirring to obtain a breathable emulsion;

[0060] L3. Place oxidized starch, glutaraldehyde, and ultrapure water into a reactor with a power of 2.7 kW. The reaction temperature is 50° C., the reaction speed is 200 r / min, and the reaction time is 2.1 h. The microporous foaming material is obtained after the reaction.

[0061] L4, the microporous foam material prepared in L3 was placed into the disease resistance enhancement preparation prepared in L1, the stirring temperature was 23°C, the stirring time was 14 min, the stirring speed was 230 r / min, after mixing, the mixture was placed in a freeze dryer with a power of 1.6 kW, the freeze drying pressure was 300 Pa, the reduced pressure drying temperature was -45°C, freeze drying was carried out for 12 h, the temperature was raised to -25°C and freeze drying was carried out for 8 h, and then freeze drying was carried out at a temperature of 15°C for 5 h to obtain a disease prevention and resistance enhancement powder;

[0062] L5. Put the disease prevention and resistance-enhancing powder prepared in L4 into the breathable emulsion prepared in L2, put it into an ultrasonic stirrer with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min, and perform ultrasonic dispersion to obtain a disease prevention and resistance-enhancing composition.

[0063] In L1, the solid-liquid ratio of salicylic acid to 50% ethanol solution is 0.15:1 g / mL.

[0064] In L3, the mass fraction of glutaraldehyde in ultrapure water is 20%.

[0065] This embodiment also provides a method for preparing a winter cabbage fungicide, which specifically comprises the following steps:

[0066] Step 1: Place methionine, methyl gallate, p-chlorophenoxyacetic acid, allantoin, and 70% ethanol solution into a 1.5 kW stirring tank at a stirring temperature of 70° C. for 14 minutes at a stirring speed of 200 r / min to obtain a plant growth promoting mixture;

[0067] Step 2: Place the high-efficiency bactericidal suspension and the disease prevention and resistance-enhancing composition into a 1.8 kW stirrer at a stirring temperature of 30° C., a stirring time of 20 min, and a stirring speed of 300 r / min to mix to obtain a high-efficiency bactericidal component;

[0068] Step 3: Add the plant growth promoting mixture prepared in step 1 to the high-efficiency bactericidal component prepared in step 2, stir at a temperature of 25°C, stir for 18 min, stir at a speed of 300 r / min, mix evenly, and obtain a winter cabbage fungicide.

[0069] In step 1, the material-liquid ratio of methionine to 70% ethanol solution is 0.13:1 g / mL.

[0070] Example 2: This example provides a winter cabbage fungicide and a preparation method thereof. The winter cabbage fungicide comprises the following components in parts by weight:

[0071] 80 parts of high-efficiency bactericidal suspension, 35 parts of disease prevention and resistance enhancement composition, 6 parts of methionine, 5 parts of methyl gallate, 8 parts of p-chlorophenoxyacetic acid, and 4 parts of allantoin.

[0072] The raw materials for preparing the high-efficiency bactericidal suspension include the following components in parts by weight: 12 parts of Herba Euphorbiae Ichthyophthirius, 8 parts of Rhizoma Cyrthospermi, 10 parts of Viola yedoensis, 6 parts of Phyllanthus urinariae, 11 parts of potassium diformate, 30 parts of citrus fiber powder, 12 parts of citric acid, 2.2 parts of trisodium citrate, 10 parts of potassium hydroxide, and 14 parts of potassium alginate.

[0073] The raw materials for preparing the disease prevention and resistance enhancement composition include the following components in parts by weight: 3 parts of salicylic acid, 4 parts of magnesium chloride, 5 parts of α-mannosidase, 21 parts of polydimethylsiloxane, 10 parts of propylene glycol, 28 parts of oxidized starch, and 15 parts of glutaraldehyde.

[0074] The preparation method of the highly effective bactericidal suspension specifically comprises the following steps:

[0075] S1. Put Herba Euphorbiae Herba, Rhizoma Cyrthospermi, Viola yedoensis, and Phyllanthus urinariae into a 1.6 kW grinder, the grinding temperature is 26°C, the grinding time is 18 min, and the grinding speed is 4000 r / min. After grinding, put it into an ultrasonic extractor with a power of 1.4 kW and a 50% ethanol solution, the extraction pressure is 1.5 MPa, the extraction frequency is 25 kHz, the extraction temperature is 43°C, the extraction time is 11 min, and after extraction, put it into a herbal concentrator with a power of 2.8 kW, the concentration temperature is 50°C, the concentration time is 40 min, and the concentration speed is 15 r / min to obtain a bactericidal and antiviral natural extract;

[0076] S2. Potassium diformate and ultrapure water were placed in a stirring tank with a power of 1.6 kW, stirred at a temperature of 28°C, a stirring time of 10 min, and a stirring speed of 160 r / min. After complete dissolution, a potassium diformate solution was obtained;

[0077] S3, placing the citrus fiber powder, the potassium diformate solution prepared in S2, citric acid, trisodium citrate, and N-methylpyrrolidone into a 2.3 kW reactor, at a reaction temperature of 95°C, a reaction time of 1.4 h, and a reaction speed of 200 r / min, to carry out an esterification and cross-linking reaction to obtain a porous matrix;

[0078] S4, put potassium hydroxide and ultrapure water into a magnetic stirrer with a power of 1.7 kW, the stirring temperature is 30°C, the stirring time is 13 min, the stirring speed is 330 r / min, and after dissolution, add the porous matrix prepared by S3, the stirring temperature is 28°C, the stirring time is 23 min, the stirring speed is 500 r / min, stir for sufficient adsorption, put into a vacuum freeze nano spray dryer with a power of 2.6 kW, the vacuum degree is -0.05 MPa, the freeze nano spray drying temperature is -40°C, the freeze nano spray drying particle size is 100 nm, the freeze nano spray drying time is 2.8 h, and freeze spray drying is performed to obtain highly efficient bactericidal nanoparticles;

[0079] S5. Place potassium alginate and ultrapure water in a 1.6 kW stirring tank at 70°C for 13 min at a stirring speed of 220 r / min and stir evenly to obtain a viscous solution.

[0080] S6. Add the bactericidal and antiviral natural extract prepared in S1 and the highly effective bactericidal nanoparticles prepared in S4 into the viscous solution prepared in S5, stir at a temperature of 33°C, a stirring time of 15 min, and a stirring speed of 270 r / min, and stir and mix to obtain a highly effective bactericidal suspension.

[0081] In S1, the solid-liquid ratio of the Herba Euphorbiae Herba to the 50% ethanol solution is 1.5:1 g / mL. In S2, the mass fraction of the potassium diformate in ultrapure water is 36%.

[0082] In S3, the material-liquid ratio of the citrus fiber powder to N-methylpyrrolidone is 0.2:1 g / mL, in S4, the mass fraction of the potassium hydroxide in ultrapure water is 39%, and in S5, the mass fraction of the potassium alginate in ultrapure water is 3%.

[0083] The method for preparing the disease prevention and resistance enhancement composition specifically comprises the following steps:

[0084] L1. Place salicylic acid, magnesium chloride, α-mannosidase, and 50% ethanol solution in a 1.3 kW stirrer at a stirring temperature of 26°C for 13 minutes at a stirring speed of 200 r / min, and mix and dissolve to obtain a disease resistance-enhancing preparation.

[0085] L2. Place polydimethylsiloxane and propylene glycol in a 2.5 kW high-speed stirrer at a high-speed stirring temperature of 28° C., a high-speed stirring speed of 7000 r / min, and a high-speed stirring time of 25 min, and emulsify them under high-speed stirring to obtain a breathable emulsion;

[0086] L3. Place oxidized starch, glutaraldehyde, and ultrapure water into a reactor with a power of 2.8 kW. The reaction temperature is 50° C., the reaction speed is 200 r / min, and the reaction time is 2.2 h. The microporous foaming material is obtained after the reaction.

[0087] L4, the microporous foam material prepared in L3 was placed into the disease resistance enhancement preparation prepared in L1, the stirring temperature was 26 ° C, the stirring time was 16 min, the stirring speed was 240 r / min, after mixing, the mixture was placed in a freeze dryer with a power of 1.9 kW, the freeze drying pressure was 300 Pa, the reduced pressure drying temperature was -45 ° C, freeze drying was carried out for 12 h, the temperature was raised to -25 ° C and freeze drying was carried out for 8 h, and then freeze drying was carried out at a temperature of 15 ° C for 5 h to obtain a disease prevention and resistance enhancement powder;

[0088] L5. Put the disease prevention and resistance-enhancing powder prepared in L4 into the breathable emulsion prepared in L2, put it into an ultrasonic stirrer with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min, and perform ultrasonic dispersion to obtain a disease prevention and resistance-enhancing composition.

[0089] In L1, the solid-liquid ratio of salicylic acid to 50% ethanol solution is 0.15:1 g / mL.

[0090] In L3, the mass fraction of glutaraldehyde in ultrapure water is 25%.

[0091] This embodiment also provides a method for preparing a winter cabbage fungicide, which specifically comprises the following steps:

[0092] Step 1: Place methionine, methyl gallate, p-chlorophenoxyacetic acid, allantoin, and 70% ethanol solution into a stirring tank with a power of 1.6 kW, stir at a temperature of 75° C., stir for 18 minutes, and stir at a speed of 230 r / min to obtain a plant growth promoting mixture;

[0093] Step 2: Place the high-efficiency bactericidal suspension and the disease prevention and resistance-enhancing composition into a 1.9 kW stirrer at a stirring temperature of 34° C. for 25 minutes at a stirring speed of 300 r / min to mix and obtain a high-efficiency bactericidal component;

[0094] Step 3: Add the plant growth promoting mixture prepared in step 1 to the high-efficiency bactericidal component prepared in step 2, stir at a temperature of 30°C, stir for 22 min, stir at a speed of 300 r / min, mix evenly, and obtain a winter cabbage fungicide.

[0095] In step 1, the material-liquid ratio of methionine to 70% ethanol solution is 0.13:1 g / mL.

[0096] Example 3: This example provides a winter cabbage fungicide and a preparation method thereof. The winter cabbage fungicide comprises the following components in parts by weight:

[0097] 90 parts of high-efficiency bactericidal suspension, 40 parts of disease prevention and resistance-enhancing composition, 8 parts of methionine, 7 parts of methyl gallate, 9 parts of p-chlorophenoxyacetic acid, and 5 parts of allantoin.

[0098] The raw materials for preparing the high-efficiency bactericidal suspension include the following components in parts by weight: 13 parts of Herba Euphorbiae Ichthyophthirius, 10 parts of Rhizoma Cyrthospermi, 12 parts of Viola yedoensis, 7 parts of Phyllanthus urinariae, 13 parts of potassium diformate, 33 parts of citrus fiber powder, 14 parts of citric acid, 2.6 parts of trisodium citrate, 12 parts of potassium hydroxide, and 16 parts of potassium alginate.

[0099] The raw materials for preparing the disease prevention and resistance enhancement composition include the following components in parts by weight: 4 parts of salicylic acid, 5 parts of magnesium chloride, 6 parts of α-mannosidase, 24 parts of polydimethylsiloxane, 11 parts of propylene glycol, 31 parts of oxidized starch, and 16 parts of glutaraldehyde.

[0100] The preparation method of the highly effective bactericidal suspension specifically comprises the following steps:

[0101] S1. Put Herba Euphorbiae Herba, Rhizoma Cyrthospermi, Viola yedoensis, and Herba Phyllanthifoliae into a 1.8 kW grinder at a grinding temperature of 28°C, a grinding time of 20 min, and a grinding speed of 4500 r / min. After grinding, put them into an ultrasonic extractor with a power of 1.6 kW and a 50% ethanol solution at an extraction pressure of 1.5 MPa, an extraction frequency of 25 kHz, an extraction temperature of 45°C, and an extraction time of 13 min. After extraction, put them into a herbal concentrator with a power of 3.5 kW, a concentration temperature of 60°C, a concentration time of 45 min, and a concentration speed of 20 r / min to obtain a bactericidal and antiviral natural extract;

[0102] S2. Potassium diformate and ultrapure water were placed in a stirring tank with a power of 1.8 kW, stirred at a temperature of 30°C, for 12 min, and at a stirring speed of 180 r / min. After complete dissolution, a potassium diformate solution was obtained;

[0103] S3, placing the citrus fiber powder, the potassium diformate solution prepared in S2, citric acid, trisodium citrate, and N-methylpyrrolidone into a 2.5 kW reactor, at a reaction temperature of 100° C., a reaction time of 1.6 h, and a reaction speed of 300 r / min, to carry out an esterification and cross-linking reaction to obtain a porous matrix;

[0104] S4, placing potassium hydroxide and ultrapure water in a magnetic stirrer with a power of 1.8 kW, stirring at a temperature of 33°C, stirring for 15 min, and stirring at a speed of 350 r / min, adding the porous matrix prepared in S3 after dissolution, stirring at a temperature of 30°C, stirring for 25 min, and stirring at a speed of 600 r / min, stirring for sufficient adsorption, placing in a vacuum freeze nano spray dryer with a power of 2.8 kW, the vacuum degree is -0.05 MPa, the freeze nano spray drying temperature is -40°C, the freeze nano spray drying particle size is 100 nm, the freeze nano spray drying time is 3 h, and freeze spray drying is performed to obtain highly efficient bactericidal nanoparticles;

[0105] S5. Place potassium alginate and ultrapure water in a 1.8 kW stirring tank at 80°C for 15 min at a stirring speed of 240 r / min and stir evenly to obtain a viscous solution.

[0106] S6. Add the bactericidal and antiviral natural extract prepared in S1 and the highly effective bactericidal nanoparticles prepared in S4 into the viscous solution prepared in S5, stir at a temperature of 36°C, a stirring time of 18 min, and a stirring speed of 280 r / min, and stir and mix to obtain a highly effective bactericidal suspension.

[0107] In S1, the solid-liquid ratio of the Herba Euphorbiae Ichthyophthirius and the 50% ethanol solution is 1.5:1 g / mL. In S2, the mass fraction of the potassium diformate in ultrapure water is 39%.

[0108] In S3, the material-liquid ratio of the citrus fiber powder to N-methylpyrrolidone is 0.2:1 g / mL, in S4, the mass fraction of the potassium hydroxide in ultrapure water is 42%, and in S5, the mass fraction of the potassium alginate in ultrapure water is 4%.

[0109] The method for preparing the disease prevention and resistance enhancement composition specifically comprises the following steps:

[0110] L1. Place salicylic acid, magnesium chloride, α-mannosidase, and 50% ethanol solution in a 1.5 kW stirrer at a stirring temperature of 28°C, a stirring time of 15 minutes, and a stirring speed of 220 r / min, and mix and dissolve to obtain a disease resistance-enhancing preparation;

[0111] L2. Place polydimethylsiloxane and propylene glycol in a high-speed stirrer with a power of 2.5 kW, set the high-speed stirring temperature at 30° C., the high-speed stirring speed at 8000 r / min, and the high-speed stirring time for 30 min, and emulsify them with high-speed stirring to obtain a breathable emulsion;

[0112] L3. Place oxidized starch, glutaraldehyde, and ultrapure water into a reactor with a power of 2.9 kW. The reaction temperature is 50° C., the reaction speed is 200 r / min, and the reaction time is 2.3 h. The microporous foaming material is obtained after the reaction.

[0113] L4, the microporous foam material prepared in L3 was placed into the disease resistance enhancement preparation prepared in L1, the stirring temperature was 28 ° C, the stirring time was 18 min, the stirring speed was 250 r / min, after mixing, the mixture was placed in a freeze dryer with a power of 2.2 kW, the freeze drying pressure was 300 Pa, the reduced pressure drying temperature was -45 ° C, freeze drying was carried out for 12 h, the temperature was raised to -25 ° C and freeze drying was carried out for 8 h, and then freeze drying was carried out at a temperature of 15 ° C for 5 h to obtain a disease prevention and resistance enhancement powder;

[0114] L5. Put the disease prevention and resistance-enhancing powder prepared in L4 into the breathable emulsion prepared in L2, put it into an ultrasonic stirrer with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min, and perform ultrasonic dispersion to obtain a disease prevention and resistance-enhancing composition.

[0115] In L1, the solid-liquid ratio of salicylic acid to 50% ethanol solution is 0.15:1 g / mL.

[0116] In L3, the mass fraction of glutaraldehyde in ultrapure water is 28%.

[0117] This embodiment also provides a method for preparing a winter cabbage fungicide, which specifically comprises the following steps:

[0118] Step 1: Place methionine, methyl gallate, p-chlorophenoxyacetic acid, allantoin, and 70% ethanol solution into a stirring tank with a power of 1.8 kW, stir at a temperature of 80° C., stir for 19 minutes, and stir at a speed of 240 r / min to obtain a plant growth promoting mixture;

[0119] Step 2: Place the high-efficiency bactericidal suspension and the disease prevention and resistance-enhancing composition into a 2.1 kW stirrer at a stirring temperature of 38° C. for 30 minutes at a stirring speed of 400 r / min to obtain a high-efficiency bactericidal component;

[0120] Step 3: Add the plant growth promoting mixture prepared in step 1 to the high-efficiency bactericidal component prepared in step 2, stir at a temperature of 35°C, stir for 26 min, stir at a speed of 400 r / min, mix evenly, and obtain a winter cabbage fungicide.

[0121] In step 1, the material-liquid ratio of methionine to 70% ethanol solution is 0.13:1 g / mL.

[0122] Comparative Example 1: This comparative example provides a cabbage fungicide and a preparation method thereof. The only difference from Example 1 is that the added high-efficiency fungicidal suspension does not contain high-efficiency fungicidal nanoparticles, and the remaining components, component contents, and method steps are the same as those in Example 1.

[0123] Comparative Example 2: This comparative example provides a cabbage fungicide and a preparation method thereof. The only difference from Example 1 is that the added disease prevention and resistance enhancement composition does not contain disease prevention and resistance enhancement powder, and the remaining components, component contents, and method steps are the same as those in Example 1.

[0124] Experimental Example 1: Test to determine the control ability and long-term control effect.

[0125] The test steps for determining the control ability and long-term control effect of the winter cabbage fungicide prepared in Examples 1-3 of the present invention are as follows:

[0126] (1) A 6-mu test greenhouse experimental field was opened, with a temperature of 5°C, a humidity of 70%, and natural light. The field was divided into a 3-mu embodiment group, a 2-mu comparative group, and a 1-mu control group. The 3-mu embodiment group's cabbage fields were applied with the winter cabbage fungicide prepared in Examples 1-3, the 2-mu comparative group's cabbage fields were applied with the cabbage fungicide prepared in Comparative Examples 1-2, and the control group's cabbage fields were applied with 75% chlorothalonil wettable powder (purchased from Shandong Bainongsida Biotechnology Co., Ltd.). Three parallel plots were set up, and the application amount was 130 g / mu. Transplanted Lubai No. 1 cabbage seedlings with a height of 6 cm were transplanted and planted at an equal distance of 16×22 cm per mu. After 8 days of planting, the winter cabbage fungicide prepared in Examples 1-3, the cabbage fungicide prepared in Comparative Examples 1-2, and the 75% chlorothalonil wettable powder of the control group were mixed with ultrapure water in a weight ratio of 1:600, dispersed evenly, and applied every 10 days. d later, a second additional application was performed;

[0127] (2) After the second application, the application of the winter cabbage fungicide prepared in Examples 1-3, the cabbage fungicide prepared in Comparative Examples 1-2, and the 75% chlorothalonil wettable powder of the control group to the Lubai No. 1 cabbage in the 6-mu test greenhouse experimental field was stopped, and the control effects on cabbage downy mildew, cabbage soft rot, and cabbage mosaic disease were recorded on the 10th, 20th, and 30th days after the application was stopped.

[0128] Result analysis:

[0129] Figure 1 This is a scanning electron microscope image of the internal structure of the highly effective bactericidal nanoparticles described in Experimental Example 1 of the present invention. Figure 2This is a graph showing the effect of controlling downy mildew on Chinese cabbage described in Experimental Example 1 of the present invention. As shown in the figure, the winter cabbage fungicide prepared in Examples 1-3, the cabbage fungicide prepared in Comparative Examples 1-2, and the 75% chlorothalonil wettable powder of the control group were applied in an amount of 130 g / mu, mixed with ultrapure water at a weight ratio of 1:600, and evenly dispersed. They were applied to transplanted Lubai No. 1 cabbage seedlings with a plant height of 6 cm, and a second additional application was performed after 10 days. On the 10th day after stopping the application, the cabbage fields of the Example 1-3 groups had a control effect of 92.8%, 94.4%, and 96.7% on downy mildew on Chinese cabbage, respectively; the cabbage fields of the Comparative Examples 1-2 groups had a control effect of 74.3% and 90.1% on downy mildew on Chinese cabbage, respectively; the cabbage fields of the control group had a control effect of 82.5% on downy mildew on downy mildew on downy mildew, and the control effect was 30% on downy mildew ... d. In the cabbage fields of Examples 1-3, the control effects of downy mildew on Chinese cabbage were 91.7%, 93.7% and 94.8% respectively. In the cabbage fields of Comparative Examples 1-2, the control effects of downy mildew on Chinese cabbage were 67.5% and 74.5% respectively. In the cabbage fields of the control group, the control effect of downy mildew on Chinese cabbage was 43.8%. Figure 3 This is a graph showing the effect of controlling soft rot of Chinese cabbage described in Experimental Example 1 of the present invention. As shown in the figure, the winter cabbage fungicide prepared in Examples 1-3, the cabbage fungicide prepared in Comparative Examples 1-2, and the 75% chlorothalonil wettable powder of the control group were applied in an amount of 130 g / mu, respectively, and mixed with ultrapure water at a weight ratio of 1:600. After uniform dispersion, they were applied to transplanted Lubai No. 1 cabbage seedlings with a plant height of 6 cm. A second additional application was performed after 10 days. On the 10th day after stopping the application, the cabbage fields of Example 1-3 groups had a control effect of 93.1%, 94.8%, and 96.7% on the cabbage fields; the cabbage fields of Comparative Examples 1-2 had a control effect of 64.4% and 91.2% on the cabbage fields; the control effect of Chinese cabbage soft rot was 72.1% on the cabbage fields of the control group; and the control effect of Chinese cabbage soft rot was 30% on the cabbage fields of the control group. d. In the cabbage fields of Examples 1-3, the control effects of Chinese cabbage soft rot were 92.5%, 94.1%, and 96.2%, respectively. In the cabbage fields of Comparative Examples 1-2, the control effects of Chinese cabbage soft rot were 63.1% and 77.1%, respectively. In the cabbage fields of the control group, the control effect of Chinese cabbage soft rot was 49.2%. Figure 4This is a result diagram of the control effect of Chinese cabbage mosaic disease described in Experimental Example 1 of the present invention. As shown in the figure, the winter cabbage fungicide prepared in Examples 1-3, the cabbage fungicide prepared in Comparative Examples 1-2, and the 75% chlorothalonil wettable powder of the control group were applied in an amount of 130 g / mu, respectively, and mixed with ultrapure water at a weight ratio of 1:600. After uniform dispersion, they were applied to transplanted Lubai No. 1 cabbage seedlings with a plant height of 6 cm. A second additional application was performed after 10 days. On the 10th day after stopping the application, the cabbage mosaic disease control effects in the cabbage fields of the Example 1-3 groups were 94.3%, 95.7%, and 97.8%, respectively. In the cabbage fields of the Comparative Examples 1-2 groups, the cabbage mosaic disease control effects were 72.6% and 90.9%, respectively. In the cabbage fields of the control group, the cabbage mosaic disease control effect was 11.3%, and on the 30th day after stopping the application d. In the cabbage fields of Examples 1-3, the control effects of Chinese cabbage mosaic disease were 93.6%, 94.5%, and 96.6%, respectively. In the cabbage fields of Comparative Examples 1-2, the control effects of Chinese cabbage mosaic disease were 71.3% and 77.0%, respectively. In the cabbage fields of the control group, the control effect of Chinese cabbage mosaic disease was 2.7%. This shows that the winter cabbage fungicide prepared by the present invention is highly effective in controlling winter cabbage diseases caused by various pathogenic microorganisms, maintains a long-term bactericidal effect, and effectively enhances the plant's own disease resistance.

[0130] Experimental Example 2: Soil pH and microbial colony influence test.

[0131] (1) A 6-mu test greenhouse experimental field was opened, with a temperature of 5°C, a humidity of 70%, and natural light. The field was divided into a 3-mu embodiment group, a 2-mu comparative group, and a 1-mu control group. The 3-mu embodiment group's cabbage fields were applied with the winter cabbage fungicide prepared in Examples 1-3, the 2-mu comparative group's cabbage fields were applied with the cabbage fungicide prepared in Comparative Examples 1-2, and the control group's cabbage fields were applied with 75% chlorothalonil wettable powder (purchased from Shandong Bainongsida Biotechnology Co., Ltd.). Three parallel plots were set up, and the application amount was 130 g / mu. Transplanted Lubai No. 1 cabbage seedlings with a height of 6 cm were transplanted and planted at an equal distance of 16×22 cm per mu. After 8 days of planting, the winter cabbage fungicide prepared in Examples 1-3, the cabbage fungicide prepared in Comparative Examples 1-2, and the 75% chlorothalonil wettable powder of the control group were mixed with ultrapure water in a weight ratio of 1:600, dispersed evenly, and applied every 10 days. The second additional application was carried out 1 day later, and a total of 7 applications were carried out. Every 10 days after application, 20 g of soil from each experimental field was taken for pH measurement. The measurement was continued for 70 days and the results were recorded.

[0132] (2) After 70 days, 10 g of soil was collected from 3 mu of cabbage field in the embodiment group, 2 mu of cabbage field in the comparative group, and 1 mu of cabbage field in the control group, with five replicates in each group.

[0133] (3) 3 mu of soil from the embodiment group, 2 mu of soil from the comparative group, and 1 mu of soil from the control group were placed in test tubes containing 1 L of sterile liquid LB culture medium, and cultured in a shaking incubator at 37°C and 120 rpm for 24 h to obtain cultured bacterial liquid;

[0134] (4) Dilute the cultured bacterial solution 100 times. In a clean bench, use a pipette to take 50 μL of the cultured bacterial solution on an LB solid culture medium plate, spread it evenly, and then invert it and culture it in a 37°C constant temperature box for 24 h. The number of bacterial colonies in the experimental group was obtained and calculated according to the formula: total bacterial colony count (cfu / g) = number of colonies on each plate × total dilution factor.

[0135] Result analysis:

[0136] Figure 5 This is a graph showing the soil pH measurement results described in Experimental Example 2 of the present invention. As shown in the figure, the winter cabbage fungicide prepared in Examples 1-3, the cabbage fungicide prepared in Comparative Examples 1-2, and the 75% chlorothalonil wettable powder in the control group were applied in an amount of 130 g / mu, mixed with ultrapure water at a weight ratio of 1:600, and after uniform dispersion, applied once every 10 d for a total of 7 times. After the first application, 20 g of soil was taken every 10 d for pH measurement. After 70 d, the soil pH values ​​of the 3 mu cabbage field in the embodiment group were 6.3, 6.3, and 6.5, respectively, the soil pH values ​​of the 2 mu cabbage field in the comparative example group were 6.3 and 6.1, respectively, and the soil pH value of the cabbage field in the control group was 5.6; Figure 6 The results of the bacterial colony count determination in the soil of Experimental Example 2 of the present invention are shown in the figure. The winter cabbage fungicide prepared in Examples 1-3, the cabbage fungicide prepared in Comparative Examples 1-2, and the 75% chlorothalonil wettable powder of the control group were applied at a rate of 130 g / mu, mixed with ultrapure water at a weight ratio of 1:600, and after uniform dispersion, applied once every 10 days for a total of 7 times. After 70 days, the bacterial colony count in the soil of the 3 mu cabbage field of the embodiment group was 9.03×10 8 cfu / g, 9.34×10 8 cfu / g, 9.54×10 8 cfu / g, and the bacterial colony counts in the 2-mu cabbage field soil of the control group were 8.96×10 8 cfu / g, 8.93×10 8 cfu / g, and the bacterial colony count in the cabbage field soil of the control group was 6.72×10 8 cfu / g, indicating that the winter cabbage fungicide prepared by the present invention will not cause soil acidification and inactivation of microorganisms in the soil, will not cause soil desertification, has biocompatibility and does not destroy the ecological balance when used for a long time.

[0137] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

[0138] The present invention and its embodiments are described above. Such description is not restrictive. The drawings show only one embodiment of the present invention, and actual applications are not limited thereto. In short, if a person skilled in the art is inspired by the above, and does not deviate from the purpose of the present invention, any method and embodiment similar to the technical solution without creative design shall fall within the scope of protection of the present invention.

Claims

1. A winter cabbage fungicide, characterized in that The winter cabbage fungicide specifically comprises the following components in parts by weight: 70-90 parts of high-efficiency fungicidal suspension, 30-40 parts of disease prevention and resistance enhancement composition, 4-8 parts of methionine, 3-7 parts of methyl gallate, 6-9 parts of p-chlorophenoxyacetic acid, and 2-5 parts of allantoin; The raw materials for preparing the high-efficiency bactericidal suspension include the following components in parts by weight: 11-13 parts of Herba Euphorbiae Ichthyophthirius, 7-10 parts of Cyperus rotundus, 8-12 parts of Viola yedoensis, 4-7 parts of Phyllanthus urinariae, 9-13 parts of potassium diformate, 26-33 parts of citrus fiber powder, 11-14 parts of citric acid, 1.8-2.6 parts of trisodium citrate, 8-12 parts of potassium hydroxide, and 12-16 parts of potassium alginate; The raw materials for preparing the disease prevention and resistance enhancement composition include the following components in parts by weight: 2-4 parts of salicylic acid, 3-5 parts of magnesium chloride, 3-6 parts of α-mannosidase, 17-24 parts of polydimethylsiloxane, 8-11 parts of propylene glycol, 25-31 parts of oxidized starch, and 13-16 parts of glutaraldehyde; The preparation method of the highly effective bactericidal suspension specifically comprises the following steps: S1. Grind Herba Euphorbiae Ichthyophthirius, Cyperus rotundus, Viola yedoensis, and Phyllanthus urinariae, extract with ultrasonic wave and concentrate with 50% ethanol solution to obtain a bactericidal and antiviral natural extract; S2, dissolving potassium diformate in ultrapure water to obtain a potassium diformate solution; S3, mixing the citrus fiber powder with the potassium diformate solution, citric acid, trisodium citrate, and N-methylpyrrolidone prepared in S2, and reacting the mixture to obtain a porous matrix; S4, dissolving potassium hydroxide in ultrapure water, adding the porous matrix prepared in S3, stirring for full adsorption, and freeze-spray drying to obtain highly efficient bactericidal nanoparticles; S5. Mix potassium alginate and ultrapure water, stir evenly, and obtain a viscous solution; S6, adding the bactericidal and antiviral natural extract prepared in S1 and the highly effective bactericidal nanoparticles prepared in S4 to the viscous solution prepared in S5, stirring and mixing to obtain a highly effective bactericidal suspension; The method for preparing the disease prevention and resistance enhancement composition specifically comprises the following steps: L1. Mix and dissolve salicylic acid, magnesium chloride, α-mannosidase and 50% ethanol solution to obtain a disease resistance-enhancing preparation; L2, emulsifying polydimethylsiloxane and propylene glycol with high-speed stirring to obtain a breathable emulsion; L3, mixing oxidized starch with glutaraldehyde and ultrapure water to react to obtain a microporous foaming material; L4, adding the microporous foam material prepared in L3 to the disease resistance enhancing preparation prepared in L1, mixing, and freeze-drying to obtain a disease prevention and resistance enhancing powder; L5. Add the disease prevention and resistance-enhancing powder prepared in L4 into the breathable emulsion prepared in L2, and perform ultrasonic dispersion to obtain a disease prevention and resistance-enhancing composition.

2. The winter cabbage fungicide according to claim 1, wherein In S1, the solid-liquid ratio of the Herba Euphorbiae Ichthyophthirius and the 50% ethanol solution is 1.5:1 g / mL. In S2, the mass fraction of the potassium diformate in ultrapure water is 35-39%.

3. The winter cabbage fungicide according to claim 2, characterized in that In S3, the material-liquid ratio of the citrus fiber powder to N-methylpyrrolidone is 0.2:1 g / mL, in S4, the mass fraction of the potassium hydroxide in ultrapure water is 36-42%, and in S5, the mass fraction of the potassium alginate in ultrapure water is 2-4%.

4. The winter cabbage fungicide according to claim 3, characterized in that In L1, the solid-liquid ratio of salicylic acid to 50% ethanol solution is 0.15:1 g / mL.

5. The winter cabbage fungicide according to claim 4, characterized in that In L3, the mass fraction of glutaraldehyde in ultrapure water is 20-28%.

6. A method for preparing the winter cabbage fungicide according to any one of claims 1 to 5, characterized in that: The specific steps include: Step 1: Mix and dissolve methionine, methyl gallate, p-chlorophenoxyacetic acid, and allantoin with a 70% ethanol solution to obtain a plant growth promoting mixture; Step 2: Mixing the high-efficiency bactericidal suspension and the disease prevention and resistance-enhancing composition to obtain a high-efficiency bactericidal component; Step 3: Add the plant growth promoting mixture prepared in step 1 to the high-efficiency bactericidal component prepared in step 2, and stir evenly to obtain a winter cabbage bactericide.

7. The preparation method of the winter cabbage fungicide according to claim 6, characterized in that: In step 1, the material-liquid ratio of methionine to 70% ethanol solution is 0.13:1 g / mL.

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