Lactobacillus plantarum for relieving and repairing skin and / or improving skin redness as well as metagen and application of lactobacillus plantarum

Abstract

The invention discloses a lactobacillus plantarum ZW1 capable of relieving and repairing skin and / or improving skin redness, the lactobacillus plantarum ZW1 is preserved in China General Microbiological Culture Collection Center (CGMCC) located in No.3, No.1 Yard, Beichen West Road, Beijing on February 27, 2023, and the preservation number of the lactobacillus plantarum ZW1 is CGMCC No.26685. The lactobacillus plantarum ZW1 can be used for relieving and repairing skin and / or improving skin redness. The lactobacillus plantarum can quickly relieve and repair the skin and improve the skin redness, the effects of relieving, repairing and improving the skin redness are good, and the onset period is short. When the lactobacillus plantarum is used as a zymophyte and lactose is used as a carbon source for fermentation, the effect of relieving, repairing and improving skin redness of the obtained metagen is remarkably improved. The lactobacillus plantarum and the metabiotics obtained by fermenting the lactobacillus plantarum can be applied to preparation of products for relieving and repairing skin and improving skin redness.

Description

Technical Field

[0001] The present invention belongs to the technical field of microorganisms, and more specifically, relates to a plant lactobacillus for soothing and repairing skin and / or improving skin redness, and a postbiotic and application thereof. Background Art

[0002] Facial redness is an important manifestation of sensitive skin. There are many factors that cause facial redness, including endogenous factors such as race, age, gender, genetics, endocrine factors, etc.; as well as exogenous factors such as chemical stimulation, environmental factors, lifestyle and psychological factors. Facial redness not only affects the beauty of the face, but also causes discomfort symptoms such as stinging and burning, which causes great trouble to people with sensitive skin. The method used to treat facial redness is generally to improve the state of reddened skin through plant or traditional Chinese medicine extracts, adding a variety of compounds with anti-inflammatory and repairing effects, and mixing a variety of chelating agents, moisturizers, thickeners, emulsifiers, moisturizers, preservative systems, etc.; this type of method has potential allergic risks for people with sensitive skin because it requires the addition of multiple chemical substances.

[0003] Lactic acid bacteria are a kind of recognized probiotics with a wide range of physiological activities. The postbiotics obtained by culturing, inactivating and decomposing lactic acid bacteria contain a variety of active ingredients, which have specific effects and a lower risk of allergies. However, different lactic acid bacteria strains have different fermentation performance and metabolic behavior, and the fermentation products and their activities will also change with different fermentation substrates. The Chinese invention patent with publication number CN114672443A discloses a strain of Lactobacillus plantarum that has the function of preventing or improving facial redness and type I rosacea. The fermentation product of its strain can improve facial redness. The facial hemoglobin detection value of healthy volunteers decreased by about 13% in 8 weeks. Its application period is long and the average improvement effect is low; and the strain, as a pure culture, has strong individual specificity and is greatly affected by the environment. It is necessary to continuously explore and explore new microorganisms with better prevention and treatment effects for treating facial redness, thereby enriching the bacterial library for the treatment of skin inflammation and providing new resources for the treatment of soothing and repairing the skin and improving skin redness. Summary of the invention

[0004] The purpose of the present invention is to explore a new bacterial strain that can more quickly and effectively soothe and repair the skin and improve skin redness; another purpose of the present invention is to provide a postbiotic and a production method thereof; a further purpose of the present invention is to provide the use of the postbiotic in the preparation of products for soothing and repairing the skin and improving skin redness.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A strain of Lactobacillus plantarum ZW1 that soothes, repairs, and / or improves skin redness. The Lactobacillus plantarum ZW1 was deposited on February 27, 2023 at the General Microbiology Center of the China Culture Collection Administration, No. 3, Yard 1, Beichen West Road, Beijing, with a deposit number of CGMCC No. 26685. The Lactobacillus plantarum ZW1 is derived from fermented fruit and vegetable samples.

[0007] The present invention discovers a strain of Lactobacillus plantarum ZW1, and the strain is classified and named as Lactobacillus plantarum by 16S rDNA identification; the strain is separated from fermented fruit and vegetable samples, can be suitable for MRS culture medium, and its colony characteristics are: the colony is round, milky white, opaque, with a diameter of about 1 to 2 mm, a smooth edge, and a moist, prominent and shiny surface; the present invention accidentally discovered in experiments that the fermentation product of the strain of Lactobacillus plantarum ZW1 can soothe and repair the skin and improve skin redness, and when the strain of Lactobacillus plantarum ZW1 is used as a fermentation bacterium and lactose is used as a carbon source for fermentation, the effect of the obtained postbiotics on soothing and repairing the skin and improving skin redness is significantly improved, therefore, the strain of Lactobacillus plantarum ZW1 and the postbiotics obtained by fermentation can be used to prepare products for soothing and repairing the skin and improving skin redness.

[0008] The present invention also provides a postbiotic, which is obtained by fermenting Lactobacillus plantarum as a fermentation bacterium, the fermentation bacterium is the above-mentioned Lactobacillus plantarum, and the fermentation uses lactose as a fermentation substrate. The postbiotic has the effect of soothing and repairing the skin and / or improving skin redness.

[0009] Furthermore, the postbiotic is prepared according to the following method: inoculating the Lactobacillus plantarum ZW1 described in claim 1 into a lactose-containing MRS medium for fermentation to obtain a primary fermentation liquid, which is the postbiotic.

[0010] Furthermore, the postbiotics are prepared according to the following method: lactose is added to the primary fermentation broth, a secondary fermentation is performed, and after the fermentation is completed, sterilization is performed to obtain the postbiotics.

[0011] Furthermore, the content of lactose in the lactose-containing MRS culture medium is 5-50 g / L; after the lactose is added, the concentration of lactose in the primary fermentation broth is 5-300 g / L.

[0012] Furthermore, the inoculation is to inoculate the seed liquid of Lactobacillus plantarum ZW1 in a lactose fermentation medium at an inoculation amount of 1-5%; and the fermentation is carried out under the conditions of culturing at 25-37° C. for 24-72 hours.

[0013] Furthermore, the secondary fermentation has the following fermentation conditions: culturing at 25-37° C. for 3-90 days.

[0014] Furthermore, before the secondary fermentation, the primary fermentation liquid needs to be heated at 40-80° C. for 10-60 minutes.

[0015] Furthermore, the formula of the lactose-containing MRS culture medium is: peptone 10.0 g / L, beef extract powder 5.0 g / L, yeast extract powder 4.0 g / L, lactose 5-50 g / L, dipotassium hydrogen phosphate 2.0 g / L, triammonium citrate 2.0 g / L, sodium acetate 5.0 g / L, magnesium sulfate 0.2 g / L, manganese sulfate 0.05 g / L, Tween 80 1.0 g / L.

[0016] The present invention provides use of the above-mentioned Lactobacillus plantarum ZW1 or the above-mentioned postbiotics in preparing products for soothing and repairing skin and / or improving skin redness.

[0017] The present invention provides a daily chemical product for soothing and repairing skin and / or improving skin redness, and the daily chemical product contains the above-mentioned postbiotics.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] (1) The strain Lactobacillus plantarum ZW1 of the present invention can quickly soothe and repair the skin and improve skin redness, has good effects in soothing and repairing the skin and improving skin redness, and has a short onset period.

[0020] (2) When the present strain of Lactobacillus plantarum ZW1 is used as a fermentation bacterium and lactose is used as a carbon source for fermentation, the effect of the obtained postbiotics on soothing, repairing the skin and improving skin redness is significantly improved.

[0021] (3) This strain of Lactobacillus plantarum and the postbiotics obtained from its fermentation can be used to prepare products that soothe, repair and improve skin redness. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is the colony morphology of Lactobacillus plantarum ZW1 inoculated on solid MRS medium.

[0023] Figure 2The effects of the first and second fermentation samples of Lactobacillus plantarum ZW1 on the survival rate of human skin fibroblasts after light damage. A represents the effects of the first and second fermentation samples of Lactobacillus plantarum ZW1 on the survival rate of human skin fibroblasts; B represents the effects of the first and second fermentation samples of ZW1 on the survival rate of human skin fibroblasts after light damage, **P<0.01, *P<0.05.

[0024] Figure 3 This is a test of the soothing and repairing effect of Lactobacillus plantarum ZW1 before fermentation and after the first and second fermentation on the skin. A represents the test of the effect of Lactobacillus plantarum ZW1 before fermentation and after the second fermentation on the redness value of the skin; B represents the test of the effect of Lactobacillus plantarum ZW1 before fermentation and after the first and second fermentation on the area of ​​the red zone and the percentage of the selected area, **P<0.01, *P<0.05.

[0025] Figure 4 This is a test of the soothing and repairing effects of the first and second fermentation samples of Lactobacillus plantarum PL02 on the skin. A represents the test of the effect of the first and second fermentation samples of Lactobacillus plantarum PL02 on the redness value of the skin; B represents the test of the effect of the first and second fermentation samples of Lactobacillus plantarum PL02 on the area of ​​the skin red zone and the percentage of the selected area. DETAILED DESCRIPTION

[0026] The present invention is further described below in conjunction with the accompanying drawings and specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0027] Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0028] Example 1 Isolation and identification of Lactobacillus plantarum ZW1 of the present invention

[0029] (1) Strain isolation

[0030] The strain ZW1 was directly obtained from Wuxi City, Jiangsu Province, and fermented fruit and vegetable samples; 1 mL of fermented fruit and vegetable fermentation liquid was added to 9 mL of normal saline, and the mixture was shaken to mix thoroughly. After gradient dilution, 10 -5 , 10 -6 , 10 -7 The fermentation broth was evenly spread on MRS plates and cultured in an anaerobic incubator at 37°C. After 24 hours, the strains were preliminarily identified by colony morphology and microscopic examination. A single strain was selected for streaking purification and culture to obtain pure colonies, which were recorded as strain ZW1.

[0031] MRS plate medium formula: glucose 20g / L, beef extract 10g / L, peptone 10g / L, yeast extract 5g / L, anhydrous sodium acetate 5g / L, diammonium citrate 2g / L, potassium dihydrogen phosphate 2g / L, manganese sulfate 0.19g / L, magnesium sulfate 0.58g / L, Tween 80 1mL, agar powder 15g / L, pH 6.5.

[0032] (2) Morphological and 16S rDNA identification of strain ZW1

[0033] The colony morphology of the strain ZW1 of the present invention is shown in Figure 1 .Depend on Figure 1 It can be seen that the colonies of strain ZW1 are round, milky white, opaque, about 1 to 2 mm in diameter, with smooth edges and a moist, prominent and shiny surface.

[0034] DNA extraction and 16S rDNA identification of strain ZW1 were performed using universal primer 27F:

[0035] 5'-AGAGTTTGATCCTGGCTCAG-3' (SEQ ID NO.1) and 1492R: 5'-GGTTACCTTGTTACGACTT-3' (SEQ ID NO.2) were used to PCR amplify the 16S rDNA fragment, the nucleotide sequence of the universal primer 27F is shown in SEQ ID NO.1, and the nucleotide sequence of the 1492R is shown in SEQ ID NO.2. 1.0% gel agar electrophoresis was used to detect the DNA extraction and the size of the PCR amplification product. The obtained target fragment was sequenced and analyzed, and the sequencing results were compared in the NCBI database. Combined with homology analysis, the strain ZW1 was determined to be Lactobacillus plantarum.

[0036] Based on the results of morphological and molecular identification, strain ZW1 was determined to be Lactobacillus plantarum and was deposited in the General Microbiology Center of China Culture Collection on February 27, 2023. The deposit address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with the deposit number CGMCC No. 26685.

[0037] Example 2 Preparation of Lactobacillus plantarum ZW1 Seed Solution (Lactobacillus plantarum ZW1 Pre-fermentation Sample)

[0038] Lactobacillus plantarum ZW1 stored at -80°C was streaked on an MRS plate in three zones and cultured at 37°C for 24 h. A single colony was picked and transferred to MRS liquid culture medium and cultured at 37°C for 24 h to obtain seed solution.

[0039] The formula of the seed culture medium used is: peptone 10.0 g / L, beef extract powder 5.0 g / L, yeast extract powder 4.0 g / L, glucose 20 g / L, dipotassium hydrogen phosphate 2.0 g / L, triammonium citrate 2.0 g / L, sodium acetate 5.0 g / L, magnesium sulfate 0.2 g / L, manganese sulfate 0.05 g / L, Tween 80 1.0 g / L. The seed solution in Example 2 is used as a sample before fermentation of Lactobacillus plantarum ZW1.

[0040] Example 3 Preparation of a single fermentation sample of Lactobacillus plantarum ZW1

[0041] The seed liquid described in Example 2 was cultured in an MRS liquid culture medium containing lactose. Specifically, the bacterial seed liquid was inoculated in an MRS liquid culture medium containing lactose at an inoculation rate of 1%, and cultured at 37° C. for 48 hours to obtain a live bacterial liquid.

[0042] The formula of the lactose-containing MRS liquid medium is: peptone 10.0 g / L, beef extract powder 5.0 g / L, yeast extract powder 4.0 g / L, lactose 20 g / L, dipotassium hydrogen phosphate 2.0 g / L, triammonium citrate 2.0 g / L, sodium acetate 5.0 g / L, magnesium sulfate 0.2 g / L, manganese sulfate 0.05 g / L, Tween 80 1.0 g / L. The fermented bacterial solution is recorded as the primary fermentation sample of Lactobacillus plantarum ZW1.

[0043] Example 4 Preparation of Lactobacillus plantarum ZW1 secondary fermentation sample

[0044] The preparation of Lactobacillus plantarum ZW1 postbiotics is prepared according to the following method:

[0045] S1. Inoculate the seed liquid of Lactobacillus plantarum ZW1 in an inoculum amount of 1% into an MRS medium containing lactose, and culture at 37°C for 48 hours to obtain a primary fermentation liquid; wherein the formula of the MRS medium containing lactose is: peptone 10.0g / L, beef extract powder 5.0g / L, yeast extract powder 4.0g / L, lactose 20g / L, dipotassium hydrogen phosphate 2.0g / L, triammonium citrate 2.0g / L, sodium acetate 5.0g / L, magnesium sulfate 0.2g / L, manganese sulfate 0.05g / L, Tween 80 1.0g / L.

[0046] S2. Add lactose to the primary fermentation broth, the concentration of lactose in the primary fermentation broth is 100 g / L, and heat at 50° C. for 15 min; then perform secondary fermentation, wherein the fermentation conditions of the secondary fermentation are: culture at 37° C. for 60 days.

[0047] After the fermentation is completed, the fermentation liquid is sterilized at 110° C. for 10 min. The sterilized bacterial liquid mixture is homogenized under high pressure for 1 h at a pressure of 10 MPa. This is the secondary fermentation sample of Lactobacillus plantarum ZW1.

[0048] Comparative Examples 1-3

[0049] Taking commercially available Lactobacillus plantarum PL02 as a control and Lactobacillus plantarum PL02 as a fermentation bacterium, the same methods as in Example 2, Example 3 and Example 4 were used to prepare Lactobacillus plantarum PL02 pre-fermentation sample, Lactobacillus plantarum PL02 primary fermentation sample and Lactobacillus plantarum PL02 secondary fermentation sample; the Lactobacillus plantarum PL02 pre-fermentation sample was recorded as Comparative Example 1; the Lactobacillus plantarum PL02 primary fermentation sample was recorded as Comparative Example 2, and the Lactobacillus plantarum PL02 secondary fermentation sample was recorded as Comparative Example 3.

[0050] Experimental Example 1: Evaluation of the improvement in soothing and repairing skin

[0051] 1. Experimental Methods

[0052] Human skin fibroblasts (HSF) were used. The cell suspension after cell recovery was placed in a 15 mL centrifuge tube with 10 mL culture medium (the cryopreservation tube was washed with culture medium to wash off all cells adhering to the wall), and centrifuged at 1000 rpm for 5 min. The supernatant was discarded and 1 mL culture medium was added to suspend the cells. The suspension was aspirated into a 10 cm culture dish containing 10 mL culture medium and gently shaken back and forth to evenly distribute the cells in the culture dish. The cells were cultured in their respective culture media, supplemented with 10% (v / v) heat-inactivated FBS, 100 U / mL penicillin and 100 μg / mL streptomycin, and cultured in a 37°C incubator in 5% CO2 for 2-3 days. After the cells adhered to the wall, the steps of subculturing were performed.

[0053] To subculture HSF cells to obtain cells with better vitality, preheat 0.25% trypsin-0.53mM EDTA digestion solution at 37℃, pour out the culture medium in the culture flask, add 3-5mL PBS to the culture flask, shake it gently and wash it, then discard it. Add 1-2mL of preheated trypsin to the flask, incubate it at 37℃ for digestion, and add 3mL of complete culture medium to terminate digestion after digestion. Use a pipette to gently blow the cells on the wall of the flask to make them completely fall off, then collect the cell suspension, centrifuge it at 1000rpm for 3min, discard the supernatant, add complete culture medium to resuspend the cells, subculture, pass the cells to several culture dishes, and continue to culture.

[0054] Cells grown to the logarithmic phase were collected, digested with 0.25% trypsin, and then seeded in 96-well culture plates (1×10 4Cell number per well). After culturing for 12 hours, the culture medium was discarded and replaced with 100 μM PA. Six parallels were set for each concentration. After culturing for 24 hours, the plant lactobacillus ZW1 primary fermentation samples and plant lactobacillus ZW1 secondary fermentation samples of Example 3 and Example 4 were added respectively. The control group was added with an equal amount of normal saline, and then incubated for 24 hours. The cell viability was detected using a CCK-8 kit. 10 μL CCK-8 solution was added to each well (be careful not to generate bubbles in the wells, as they will affect the reading of the OD value). The culture plate was incubated in an incubator for 2 hours. The absorbance at 450 nm was measured using an ELISA reader.

[0055] To establish the HSF light damage model, HSF cells were cultured at a rate of 1×10 4 / well were inoculated into 96-well culture plates and cultured in a CO2 cell culture incubator. When the cells grew to 60-70% adherent to the wall, the original culture medium was discarded, and 100 μL / well of serum-free culture medium was added for starvation treatment for 12 h. The culture medium was removed and the plates were washed twice with PBS. The 96-well plates were placed in a UV cross-linker for UVB irradiation. The radiation doses of the UV cross-linker were set to 0 mJ·cm-2, 20 mJ·cm-2, and 100 μL / well of serum-free culture medium. -2 , 40mJ·cm -2 , 60mJ·cm -2 , 80mJ·cm -2 After irradiation, the cells were incubated in a CO2 cell culture incubator for 24 h, and cell viability was detected using CCK-8 to screen for a suitable radiation dose. In this embodiment, 60 mJ·cm -2 , establish a UVB light damaged cell model.

[0056] According to the UVB light damaged cell model, HSF cells were passaged according to the above method to obtain cells with better vitality, and the cells grown to the logarithmic phase were collected and divided into a control group, a model group, a primary fermentation group, and a secondary fermentation group; the control group did not receive any treatment; the model group was irradiated with the above radiation dose; the primary fermentation group was added with the primary fermentation sample of Lactobacillus plantarum ZW1 before irradiation; the secondary fermentation group was added with the secondary fermentation sample of Lactobacillus plantarum ZW1 before irradiation, and the cell vitality was detected by CCK-8, and the cell survival rate was recorded.

[0057] 2. Experimental results

[0058] like Figure 2 As shown in A, the secondary fermentation samples of Lactobacillus plantarum ZW1 are almost non-toxic to HSF cells. The secondary fermentation samples of Lactobacillus plantarum ZW1 are used in the HSF light damage model. Figure 2As shown in B, the cell survival rate of the model group was reduced by about 50% compared with that of the control group. The cell survival rate was increased by 20% by adding the first fermentation sample of Lactobacillus plantarum ZW1, and the cell survival rate was increased by 25% by adding the second fermentation sample of Lactobacillus plantarum ZW1, indicating that the first fermentation sample of Lactobacillus plantarum ZW1 and the second fermentation sample of Lactobacillus plantarum ZW1 have the effect of significantly improving the photodamage of HSF cells induced by UVB.

[0059] Experimental Example 2 Evaluation of the improvement of skin red area and redness value

[0060] 1. Experimental Methods

[0061] Recruit subjects with skin redness, and retain subjects with similar redness symptoms by taking photos and analyzing the redness value; Randomly select 40 subjects, take photos of the subjects' faces to observe the redness of the skin to statistically analyze the changes in the skin red area and redness value index data before and after using the samples, so as to evaluate the effect of the test sample on improving skin redness. The tested samples are: the pre-fermentation sample of plant lactobacillus ZW1 in Example 2, the first fermentation sample of plant lactobacillus ZW1 in Example 3, the second fermentation sample of plant lactobacillus ZW1 in Example 4, and the plant lactobacillus PL02 pre-fermentation sample, plant lactobacillus PL02 first fermentation sample and plant lactobacillus PL02 second fermentation sample prepared by comparative examples 1-3, and the control group is treated with clean water. Before using the sample, the subject was revisited in the first and second weeks of using the sample. The test conditions were all controlled in a constant temperature and humidity environment, and the light was constant. The subject did not apply any skin care products and cosmetics after cleaning the face on the day of the test, and started the test after sitting for 30 minutes with clean water cleansing: the subject was photographed with a facial image analyzer, and the left face, front face and right face of the subject were photographed respectively. During the test period, the above postbiotic samples were used to replace the daily cream products, and the samples were used as required (once in the morning and once in the evening). The change in the percentage of the red area of ​​the subject's skin and the selected area was tested. The smaller the value, the better the soothing effect of the sample; the redness value of the subject's skin was tested. The lower the value, the better the soothing effect of the sample.

[0062] 2. Experimental results

[0063] like Figure 3As shown in A, the skin redness value of the sample before fermentation of Lactobacillus plantarum ZW1 decreased by 5.1% after one week of use, and decreased by 6% after two weeks of use, indicating that the Lactobacillus plantarum ZW1 bacteria before fermentation also has a certain improvement effect on the redness value of the skin. The redness value of the skin decreased more significantly after one week and two weeks of use, respectively. The skin redness value of the Lactobacillus plantarum ZW1 single fermentation sample decreased by 18.91% after one week of use. Compared with the bacterial sample before fermentation of Lactobacillus plantarum ZW1, the Lactobacillus plantarum ZW1 single fermentation sample had a more obvious improvement in skin soothing after one week of use. Compared with the Lactobacillus plantarum ZW1 single fermentation sample, the skin redness value of the Lactobacillus plantarum ZW1 secondary fermentation sample decreased by nearly 3% after one week and two weeks of use. It shows that the Lactobacillus plantarum ZW1 single fermentation sample has a better soothing and improving effect on the skin than the Lactobacillus plantarum ZW1 single fermentation sample.

[0064] like Figure 3 As shown in B, the red area and the percentage of the selected area of ​​the sample before fermentation of Lactobacillus plantarum ZW1 decreased by 2.7% after one week of use, indicating that the Lactobacillus plantarum ZW1 thalline before fermentation also has a certain improvement effect on the red area of ​​the skin. After one week of use, the red area and the percentage of the selected area of ​​the sample of Lactobacillus plantarum ZW1 once fermented sample decreased by 10.95%, and the red area and the percentage of the selected area of ​​the control group decreased by 2.18%. Compared with the sample before fermentation of Lactobacillus plantarum ZW1, the experimental sample of the present invention improved the skin soothing improvement degree after one week of use more obviously. The Lactobacillus plantarum ZW1 secondary fermented sample, relative to the Lactobacillus plantarum ZW1 once fermented sample, decreased by nearly 5% after one week of use of the red area and the percentage of the selected area of ​​the sample, and decreased by about 8% after two weeks of use of the red area and the percentage of the selected area of ​​the sample. It shows that the soothing and improving effect of the sample after the secondary fermentation of Lactobacillus plantarum ZW1 on the skin is more significant than the Lactobacillus plantarum ZW1 once fermented sample.

[0065] The evaluation results of the samples before fermentation of Lactobacillus plantarum PL02, the samples of the first fermentation of Lactobacillus plantarum PL02, and the samples of the second fermentation of Lactobacillus plantarum PL02 obtained in Comparative Examples 1-3 are as follows: Figure 4 As shown, both the skin redness value and the percentage of the red area to the selected area showed almost no decreasing trend compared with the control group, indicating that the PL02 fermentation sample does not have an efficient soothing and repairing effect.

[0066] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A strain of Lactobacillus plantarum ZW1, characterized in that: The Lactobacillus plantarum ZW1 was deposited on February 27, 2023 at the General Microbiology Center of the China National Microbiological Culture Collection Administration, No. 3, Yard 1, Beichen West Road, Beijing, and its deposit number is CGMCC No. 26685.

2. A postbiotic, characterized in that: Obtained by fermentation of the Lactobacillus plantarum ZW1 described in claim 1.

3. The postbiotic according to claim 2, characterized in that The fermentation uses lactose as a fermentation substrate.

4. The postbiotic according to claim 3, characterized in that The preparation method is as follows: the plant lactobacillus ZW1 described in claim 1 is inoculated into a lactose-containing MRS medium for fermentation to obtain a primary fermentation liquid, which is the postbiotic.

5. The postbiotic according to claim 4, characterized in that The preparation method is as follows: lactose is added to the primary fermentation liquid, a secondary fermentation is carried out, and after the fermentation is completed, sterilization is performed to obtain the postbiotics.

6. The postbiotic according to claim 4, characterized in that The content of lactose in the lactose-containing MRS culture medium is 5-50 g / L; after the lactose is added, the concentration of lactose in the primary fermentation liquid is 5-300 g / L.

7. The postbiotic according to claim 4, characterized in that The inoculation is to inoculate the seed liquid of Lactobacillus plantarum ZW1 into the lactose fermentation medium at an inoculation amount of 1-5%; the fermentation is carried out under the conditions of culturing at 25-37° C. for 24-72 hours.

8. The postbiotic according to claim 5, characterized in that The secondary fermentation has the following fermentation conditions: culturing at 25-37° C. for 3-90 days.

9. Use of Lactobacillus plantarum ZW1 according to claim 1 or the postbiotic according to any one of claims 2 to 8 in the preparation of a product for soothing and repairing skin and / or improving skin redness.

10. A daily chemical product for soothing and repairing skin and / or improving skin redness, characterized in that: Containing the postbiotic according to any one of claims 2 to 8.

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