A Microscope Objective and Method for Protein Detection

Abstract

The present invention provides a microscope objective lens and method for protein detection, which relates to the technical field of microscopes. The microscope objective lens includes a first lens holder, a second lens holder, an elastic element mounting groove, a limiting block, a trigger protection block and an elastic element. The retraction function of the second lens holder after the front end face of the objective lens contacts the protein stage is realized through the trigger protection block, so as to achieve the purpose of protecting the microscope objective lens. The elastic element mounting groove provides axial limitation and guidance for the second lens holder and provides an installation position for the elastic element, solving the problem that the existing protection measures of microscope objective lenses rely on improving the risk prevention awareness of operators and do not achieve the protection purpose from the direction of optimizing the objective lens structure.

Description

Technical Field

[0001] The present invention relates to the technical field of microscopes, and particularly to a microscope objective lens and method for protein detection. Background Art

[0002] With the rapid development of the biotechnology field, the requirements for detection technologies such as gene detection, protein detection, and cell detection are also continuously increasing; in the field of protein detection, microscope observation requires precise adjustment of the position and focal length of the objective lens to obtain a clear image; however, due to the fragility of protein samples and the precision characteristics of the detection system, improper operation may cause the objective lens to contact the sample, and such contact will significantly reduce the microscope performance and even damage the microscope.

[0003] According to data surveys, about 5%-10% of protein detection laboratories have reported such incidents, and more than 60% of these cases have led to objective lens damage to varying degrees. Existing protective measures for microscope objective lenses rely on improving the risk prevention awareness of operators and do not achieve the protection purpose from the direction of optimizing the objective lens structure. Therefore, optimizing the structure of the microscope objective lens for protein detection plays an important role in minimizing the risk of objective lens damage. Summary of the Invention

[0004] Therefore, the technical problem to be solved by the present invention is to overcome the defects in the prior art, so as to provide a microscope objective lens and method for protein detection.

[0005] A microscope objective lens for protein detection, comprising: a lens base and a lens group. The lens base includes a first lens base and a second lens base arranged in sequence. An elastic element installation groove and a limiting block are provided at one end of the first lens base close to the second lens base. An elastic element is arranged in the elastic element installation groove. A guiding groove is provided on the limiting block. The second lens base is installed in cooperation with the elastic element installation groove. A strip-shaped guiding block corresponding to the guiding groove is provided on the second lens base. The strip-shaped guiding block is installed in cooperation with the guiding groove. The lens group includes a first lens group and a second lens group. The first lens group is installed in the first lens base, and the second lens group is installed in the second lens base. A trigger protection block is provided at one end of the second lens base far from the first lens base. A round chamfer structure is provided on the trigger protection block. The protruding length of the trigger protection block is greater than the protruding length of the second lens group.

[0006] Further, the first lens group includes a stress absorption unit and a fourth lens arranged horizontally in sequence. The fourth lens is arranged at one end of the first lens base close to the second lens base. The stress absorption unit is installed on the first lens base, and the stress absorption end of the stress absorption unit contacts the fourth lens.

[0007] Further, the stress absorption unit is a first flexible link. A first opto-mechanical interface is provided on the first flexible link. The first flexible link is installed on the first lens base, and the first opto-mechanical interface contacts the fourth lens.

[0008] Further, the second lens group includes an axially pressing unit and a sixth lens arranged transversely in sequence. The sixth lens is arranged at one end of the second lens holder close to the trigger protection block. The axially pressing unit is mounted on the second lens holder, and the pressing end of the axially pressing unit contacts the sixth lens.

[0009] Further, the axially pressing unit is a second flexible link. A second opto-mechanical interface is provided on the second flexible link. The second flexible link is mounted on the second lens holder, and the second opto-mechanical interface contacts the sixth lens.

[0010] Further, the first lens group further includes a first retaining ring, a first lens, a first spacer ring, a second lens, a second spacer ring, a third lens, and a third spacer ring connected transversely in sequence. The third spacer ring is connected to the fourth lens, and the first flexible link is mounted on the third spacer ring.

[0011] Further, the second lens group further includes a second retaining ring, a fifth lens, and a fourth spacer ring connected transversely in sequence. The fourth spacer ring is connected to the sixth lens, and the second flexible link is mounted on the fourth spacer ring.

[0012] Further, the microscopic objective lens further includes a first outer lens barrel, a second outer lens barrel, and a third outer lens barrel. The first outer lens barrel and the third outer lens barrel are both mounted on the first lens holder. The second lens holder is mounted in the third outer lens barrel. The second outer lens barrel is connected to both the first outer lens barrel and the third outer lens barrel at the same time. A through guiding groove corresponding to the trigger protection block is provided at one end of the third outer lens barrel close to the trigger protection block, and the trigger protection block is fitted and mounted with the through guiding groove.

[0013] Further, the elastic element is a compression spring, the elastic element mounting groove is a spring groove, the spring groove is provided at one end of the first lens holder close to the second lens holder, the compression spring is mounted in the spring groove, the trigger protection block is an extended strip-shaped block, and the extended strip-shaped block is mounted at one end of the second lens holder away from the first lens holder.

[0014] The present invention further includes a lens protection method for a microscopic objective lens for protein detection. This method is implemented based on a microscopic objective lens for protein detection as described in any one of the above. After the trigger protection block contacts the protein stage surface, the trigger protection block drives the second lens holder and the second lens group to move along the elastic element mounting groove in the direction close to the first lens holder. At the same time, the strip-shaped guiding block moves along the guiding groove in the direction close to the first lens holder, and the elastic element compresses and stores energy. After the trigger protection block is separated from the protein stage surface, the elastic element expands and releases energy. The trigger protection block drives the second lens holder and the second lens group to move along the elastic element mounting groove in the direction away from the first lens holder. At the same time, the strip-shaped guiding block moves along the guiding groove in the direction away from the first lens holder, and the second lens holder and the second lens group return to their original positions.

[0015] The technical solution of the present invention has the following advantages:

[0016] 1. The technical solution provided by the present invention optimizes the structure for the protection function of the microscope lens. By triggering the protection block, the retraction function of the second lens seat is realized after the front end face of the objective lens contacts the protein stage. Without introducing redundant stresses that may cause damage or reduced accuracy of the objective lens, the purpose of protecting the microscope objective lens is achieved. The elastic element mounting groove provides axial limitation and guidance for the second lens seat and provides a mounting position for the elastic element. The strip-shaped guide block and the guide groove further limit the remaining degrees of freedom of the second lens seat other than axial limitation. When the microscope objective lens receives a forced displacement in the radial direction, the circular chamfer structure enables the trigger protection block to also drive the objective lens to achieve axial expansion and contraction. The elastic element stores energy when the second lens seat retracts. After the trigger protection block is separated from contact with the protein stage, the elastic element can expand and release energy to ensure that the second lens seat returns to its original position. The protruding length of the trigger protection block is greater than the protruding length of the second lens group to ensure that the trigger protection block always contacts the protein stage prior to the second lens group, preventing damage to the second lens group.

[0017] 2. In the technical solution provided by the present invention, the contact between the first opto-mechanical interface and the fourth lens causes the first flexible link to generate reverse stress and deform to a corresponding size. The deformation of the first flexible link can reduce the optical surface stress of the fourth lens and simultaneously absorb the axial distributed stress generated by the elastic element pressing the second lens seat after the objective lens is assembled. The first flexible link also provides an axial flexible pressing force for the fourth lens.

[0018] 3. In the technical solution provided by the present invention, the second flexible link provides axial flexible pressing support for the sixth lens, and also reduces the optical surface stress of the sixth lens and absorbs the stress on the sixth lens after the objective lens is assembled. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for the description of the specific embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0020] Figure 1 It is a cross-sectional view of the overall structure of the present invention;

[0021] Figure 2 It is a schematic diagram of the strip-shaped guide block and the extended strip-shaped block structure of the present invention;

[0022] Figure 3 It is a schematic diagram of the guide groove and the spring groove structure of the present invention;

[0023] Figure 4 This is a schematic diagram of the first flexible link and the first opto-mechanical interface structure of the present invention.

[0024] Description of the reference numerals:

[0025] 1 - First outer lens barrel; 2 - Second outer lens barrel; 3 - Third outer lens barrel; 4 - First lens holder; 5 - Second lens holder; 6 - First retaining ring; 7 - First lens; 8 - First spacer ring; 9 - Second lens; 10 - Second spacer ring; 11 - Third lens; 12 - Third spacer ring; 13 - Fourth lens; 14 - Second retaining ring; 15 - Fifth lens; 16 - Fourth spacer ring; 17 - Sixth lens; 18 - Compression spring; 19 - Strip-shaped guide block; 20 - Extended strip-shaped block; 21 - Round chamfer structure; 22 - Guide groove; 23 - Spring groove; 24 - Limiting table surface; 25 - First flexible link; 26 - First opto-mechanical interface. Detailed implementation manners

[0026] Next, the technical solutions of the present invention will be clearly and completely described in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0027] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the accompanying drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention. In addition, the terms "first", "second", "third" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0028] In the description of the present invention, it should be noted that unless otherwise clearly specified and limited, the terms "installed", "connected", "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0029] In addition, the technical features involved in different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0030] Such as Figures 1 to 3A microscopic objective lens for protein detection as shown includes: a lens base and a lens group. The lens base includes a first lens base 4 and a second lens base 5 arranged in sequence. An elastic element installation groove and a limiting block are provided at one end of the first lens base 4 close to the second lens base 5. An elastic element is arranged in the elastic element installation groove. A guiding groove 22 is formed on the limiting block. The second lens base 5 is installed in cooperation with the elastic element installation groove. A strip-shaped guiding block 19 corresponding to the guiding groove 22 is arranged on the second lens base 5. The strip-shaped guiding block 19 is installed in cooperation with the guiding groove 22. The lens group includes a first lens group and a second lens group. The first lens group is installed in the first lens base 4, and the second lens group is installed in the second lens base 5. A trigger protection block is provided at one end of the second lens base 5 away from the first lens base 4. A circular chamfer structure 21 with a size of 0.5 mm is arranged on the trigger protection block. The protruding length of the trigger protection block is greater than the protruding length of the second lens group.

[0031] For the above microscopic objective lens for protein detection, the structure is optimized for the protection function of the microscope lens. The retraction function of the second lens base 5 after the front end face of the objective lens contacts the protein tabletop is realized through the trigger protection block. On the premise of not introducing redundant stresses that may cause damage to the objective lens or reduction in accuracy, the purpose of protecting the microscopic objective lens is achieved. The elastic element installation groove provides axial limitation and guidance for the second lens base 5 and provides an installation position for the elastic element. The strip-shaped guiding block 19 and the guiding groove 22 further limit the remaining degrees of freedom of the second lens base 5 except for the axial limitation. When the microscopic objective lens receives a forced displacement in the radial direction, the circular chamfer structure 21 can also drive the objective lens to achieve axial expansion and contraction when the trigger protection block is triggered by a collision within a distance of 0.5 mm above the protein tabletop. The elastic element stores energy when the second lens base 5 retracts. After the trigger protection block is separated from the contact with the protein tabletop, the elastic element can expand and release energy to ensure that the second lens base 5 returns to its original position. The elastic element can also provide a pressing force on the first lens base 4 and the second lens base 5 at the same time to restrict the degree of freedom of axial translation of the second lens base 5. The protruding length of the trigger protection block is greater than the protruding length of the second lens group, ensuring that the trigger protection block always contacts the protein tabletop before the second lens group, preventing damage to the second lens group and also preventing damage to the microscopic objective lens.

[0032] Such as Figures 1 to 4As shown in the figure, in this embodiment, the first lens group includes a stress absorption unit and a fourth lens 13 arranged horizontally in sequence. The fourth lens 13 is arranged at one end of the first lens holder 4 close to the second lens holder 5. The stress absorption unit is installed on the first lens holder 4, and the stress absorption end of the stress absorption unit contacts the fourth lens 13. The stress absorption unit is specifically a first flexible link 25. A first opto-mechanical interface 26 is provided on the first flexible link 25. The first flexible link 25 is installed on the first lens holder 4, and the first opto-mechanical interface 26 contacts the fourth lens 13. The contact between the first opto-mechanical interface 26 and the fourth lens 13 causes the first flexible link 25 to generate reverse stress and deform in corresponding dimensions. The deformation of the first flexible link 25 can reduce the optical surface stress of the fourth lens 13 and at the same time absorb the axial distributed stress generated by the elastic element pressing the second lens holder 5 after the objective lens is assembled. The first flexible link 25 also provides an axial flexible pressing force for the fourth lens 13.

[0033] As Figures 1 to 2 shown in the figure, in this embodiment, the second lens group includes an axial pressing unit and a sixth lens 17 arranged horizontally in sequence. The sixth lens 17 is arranged at one end of the second lens holder 5 close to the trigger protection block. The axial pressing unit is installed on the second lens holder 5, and the pressing end of the axial pressing unit contacts the sixth lens 17. The axial pressing unit is specifically a second flexible link. A second opto-mechanical interface is provided on the second flexible link. The second flexible link is installed on the second lens holder 5, and the second opto-mechanical interface contacts the sixth lens 17. The second flexible link provides axial flexible pressing support for the sixth lens 17, and at the same time reduces the optical surface stress of the sixth lens 17 and absorbs the stress on the sixth lens after the objective lens is assembled. It should be noted that the structure of the second flexible link is the same as that of the first flexible link 25, and the structure of the second opto-mechanical interface is the same as that of the first opto-mechanical interface 26.

[0034] As Figure 1 、 Figure 3 and Figure 4 shown in the figure, in this embodiment, the first lens group further includes a first retaining ring 6, a first lens 7, a first spacer 8, a second lens 9, a second spacer 10, a third lens 11, and a third spacer 12 that are horizontally connected in sequence along the Figure 1 optical axis shown in the figure. The third spacer 12 is connected to the fourth lens 13, and the first flexible link 25 is installed on the third spacer 12. All the above components are tightly connected. Among them, the first retaining ring 6 is screwed to the inner wall surface of the first lens holder 4 to press the first lens 7, the first spacer 8, the second lens 9, the second spacer 10, the third lens 11, and the third spacer 12 to ensure the positional relationship between the components and provide axial position limitation. A plurality of first flexible links 25 are evenly distributed in the radial direction of the inner wall surface of the third spacer 12. The specific installation position and quantity of the first flexible links 25 are determined according to actual design and use requirements.

[0035] As Figure 1 and Figure 2 shown, in this embodiment, the second lens group further includes a second retaining ring 14, a fifth lens 15, and a fourth spacer 16 that are sequentially connected transversely along the Figure 1 optical axis shown in. The fourth spacer 16 is connected to the sixth lens 17, and the second flexible link is installed on the fourth spacer 16; the second retaining ring 14 is screwed to the inner wall surface of the second lens holder 5 to press the fifth lens 15 and the fourth spacer 16, ensuring the positional relationship among the fifth lens 15, the fourth spacer 16, and the sixth lens 17 and providing axial position limitation; a plurality of second flexible links are evenly distributed in the radial direction of the inner wall surface of the fourth spacer 16, and the specific installation positions and quantities of the second flexible links are determined according to actual design and usage requirements.

[0036] As Figures 1 to 3 shown, in this embodiment, the microscope objective further includes a first outer lens barrel 1, a second outer lens barrel 2, and a third outer lens barrel 3. The first outer lens barrel 1 and the third outer lens barrel 3 are both installed on the first lens holder 4, the second lens holder 5 is installed inside the third outer lens barrel 3, the second outer lens barrel 2 is connected to both the first outer lens barrel 1 and the third outer lens barrel 3 at the same time. A through guiding groove corresponding to the trigger protection block is provided at one end of the third outer lens barrel 3 close to the trigger protection block, and the trigger protection block is installed in cooperation with the through guiding groove; an external thread is provided on the first outer lens barrel 1, an internal thread is provided on the second outer lens barrel 2, and the external thread on the first outer lens barrel 1 is connected to the internal thread on the second outer lens barrel 2 to realize the threaded connection between the first outer lens barrel 1 and the second outer lens barrel 2. An external thread is provided on the third outer lens barrel 3, and the external thread on the third outer lens barrel 3 is connected to the internal thread on the second outer lens barrel 2 to realize the threaded connection between the third outer lens barrel 3 and the second outer lens barrel 2. Rotating the second outer lens barrel 2 can provide the ability to adjust the axial displacement, and rotating the third outer lens barrel 3 can also provide the ability to adjust the axial displacement. The first outer lens barrel 1, the second outer lens barrel 2, and the third outer lens barrel 3 are connected to each other by threaded connections to form the main frame of the microscope objective. In addition, the first outer lens barrel 1, the second outer lens barrel 2, and the third outer lens barrel 3 are all made of aluminum alloy material. The main material of the microscope objective is set as aluminum alloy material because the aluminum alloy material has good cutting performance and heat dissipation performance and is suitable for mass production, so it is suitable for application to the main frame of the microscope objective. Four through guiding grooves corresponding to the trigger protection block are radially distributed on one end face of the third outer lens barrel 3 close to the trigger protection block, and the trigger protection block is installed in cooperation with the through guiding groove, and the trigger protection block is slidably arranged in the through guiding groove.

[0037] As Figures 1 to 3As shown in the figure, in this embodiment, the elastic element is a compression spring 18, the elastic element mounting groove is a spring groove 23, the spring groove 23 is provided at one end of the first lens holder 4 close to the second lens holder 5, the compression spring 18 is installed in the spring groove 23, the trigger protection block is an extended strip block 20, and the extended strip block 20 is installed at one end of the second lens holder 5 away from the first lens holder 4; the spring groove 23 is used to assemble the compression spring 18, and at the same time, the spring groove 23 is also used to provide guidance and limit for the movement of the second lens holder 5. When the compression spring 18 is in a compressed and tightened state, the compression spring 18 simultaneously provides a pressing force on the first lens holder 4 and the second lens holder 5, so as to achieve the purpose of restricting the axial translation degree of freedom of the second lens holder 5. When the strip-shaped guide block 19 is installed in cooperation with the guide groove 22 to achieve nested cooperation, all other degrees of freedom of the second lens holder 5 except for the axial displacement are restricted. After being screwed to the optical target position, the first lens holder 4 and the second lens holder 5 are fixed in the first outer lens barrel 1, the second outer lens barrel 2, and the third outer lens barrel 3. The extended length of the extended strip block 20 is greater than the extended length of the second lens group, that is, the end surface of the extended strip block 20 is higher than the end surface of the sixth lens 17. In this embodiment, the end surface of the extended strip block 20 is 0.2 mm higher than the end surface of the sixth lens 17. However, the specific height data can be adjusted according to the actual use design requirements to ensure that the extended strip block 20 always contacts the protein stage first and then retracts accordingly to protect the sixth lens 17 and prevent the sixth lens 17 from being damaged when contacting the protein stage.

[0038] As Figures 1 to 3 shown, the present invention further includes a lens protection method for a protein detection microscope objective, which is realized based on any one of the above-mentioned protein detection microscope objectives. After the trigger protection block contacts the protein stage, the trigger protection block drives the second lens holder 5 and the second lens group to move along the elastic element mounting groove towards the direction close to the first lens holder 4. At the same time, the strip-shaped guide block 19 moves along the guide groove 22 towards the direction close to the first lens holder 4, and the elastic element compresses and stores energy; after the trigger protection block is separated from the protein stage, the elastic element expands and releases energy, and the trigger protection block drives the second lens holder 5 and the second lens group to move along the elastic element mounting groove towards the direction away from the first lens holder 4. At the same time, the strip-shaped guide block 19 moves along the guide groove 22 towards the direction away from the first lens holder 4, and the second lens holder 5 and the second lens group return to their original positions;

[0039] Specifically:

[0040] After the extended bar 20 comes into contact with the protein stage, the extended bar 20 drives the second lens holder 5 and the second lens group to move along the spring groove 23 in the direction close to the first lens holder 4. At the same time, the bar-shaped guide block 19 moves along the guide groove 22 in the direction close to the first lens holder 4. The internal stress of the microscope objective is converted into the elasticity of the compression spring 18, and the compression spring 18 compresses to store energy. When the extended bar 20 is separated from the protein stage and the relative distance between the extended bar 20 and the protein stage increases, the compression spring 18 expands to release energy. The extended bar 20 drives the second lens holder 5 and the second lens group to move along the spring groove 23 in the direction away from the first lens holder 4. At the same time, the bar-shaped guide block 19 moves along the guide groove 22 in the direction away from the first lens holder 4, and the second lens holder 5 and the second lens group return to their original positions.

[0041] The length of the compression spring 18 in the stress-free state is 10 mm. The distance from the end face of the second lens holder 5 close to the first lens holder 4 to the bottom surface of the spring groove 23 is 5 mm. At this time, the compressed length of the compression spring 18 after being installed on the spring groove 23 is 5 mm. The end face of the first lens holder 4 provided with the limit block and the guide groove 22 is the limit table surface 24, and the minimum distance from the limit table surface 24 to the bar-shaped guide block 19 is 3 mm. That is, when the extended bar 20 drives the second lens holder 5 and the second lens group to move along the spring groove 23 in the direction close to the first lens holder 4, since the minimum distance from the limit table surface 24 to the bar-shaped guide block 19 is 3 mm, the compression range of the compression spring 18 is limited at this time. The maximum distance that the extended bar 20 drives the second lens holder 5 and the second lens group to move along the spring groove 23 in the direction close to the first lens holder 4 is 3 mm. However, the above data are only applied in this embodiment, and the specific distance can be adjusted accordingly according to the actual use design requirements.

[0042] Obviously, the above embodiments are only examples for clear illustration and are not limitations on the implementation manners. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to enumerate all implementation manners here. The obvious changes or modifications derived therefrom are still within the protection scope of the present invention.

Claims

1. A microscopic objective lens for protein detection, comprising: A lens holder and a lens group, characterized in that the lens holder comprises a first lens holder (4) and a second lens holder (5) arranged in sequence, one end of the first lens holder (4) close to the second lens holder (5) is provided with an elastic element mounting groove and a limiting block, an elastic element is arranged in the elastic element mounting groove, a guiding groove (22) is formed in the limiting block, the second lens holder (5) is mounted in cooperation with the elastic element mounting groove, a strip-shaped guiding block (19) corresponding to the guiding groove (22) is arranged on the second lens holder (5), the strip-shaped guiding block (19) is mounted in cooperation with the guiding groove (22), the lens group comprises a first lens group and a second lens group, the first lens group is mounted in the first lens holder (4), the second lens group is mounted in the second lens holder (5), a trigger protection block is arranged at one end of the second lens holder (5) far from the first lens holder (4), a round chamfer structure (21) is arranged on the trigger protection block, the protruding length of the trigger protection block is greater than the protruding length of the second lens group, after the trigger protection block contacts the protein tabletop, the trigger protection block drives the second lens holder (5) and the second lens group to move along the elastic element mounting groove towards the direction close to the first lens holder (4).

2. The micro objective lens for protein detection according to claim 1, characterized in that, The first lens group comprises a stress absorption unit and a fourth lens (13) arranged horizontally in sequence, the fourth lens (13) is arranged at one end of the first lens holder (4) close to the second lens holder (5), the stress absorption unit is mounted on the first lens holder (4), and the stress absorption end of the stress absorption unit contacts the fourth lens (13).

3. The micro objective lens for protein detection according to claim 2, characterized in that, The stress absorption unit is a first flexible link (25), a first opto-mechanical interface (26) is arranged on the first flexible link (25), the first flexible link (25) is mounted on the first lens holder (4), and the first opto-mechanical interface (26) contacts the fourth lens (13).

4. A microscopic objective lens for protein detection according to claim 1, characterized in that, The second lens group comprises an axial pressing unit and a sixth lens (17) arranged horizontally in sequence, the sixth lens (17) is arranged at one end of the second lens holder (5) close to the trigger protection block, the axial pressing unit is mounted on the second lens holder (5), and the pressing end of the axial pressing unit contacts the sixth lens (17).

5. The microscopic objective lens for protein detection according to claim 4, wherein, The axial pressing unit is a second flexible link, a second opto-mechanical interface is arranged on the second flexible link, the second flexible link is mounted on the second lens holder (5), and the second opto-mechanical interface contacts the sixth lens (17).

6. The microscopic objective lens for protein detection according to claim 2, characterized in that, The first lens group further comprises a first retaining ring (6), a first lens (7), a first spacer ring (8), a second lens (9), a second spacer ring (10), a third lens (11) and a third spacer ring (12) connected horizontally in sequence, the third spacer ring (12) is connected to the fourth lens (13), and the first flexible link (25) is mounted on the third spacer ring (12).

7. A microscope objective lens for protein detection according to claim 4, characterized in that, The second lens group further comprises a second retaining ring (14), a fifth lens (15) and a fourth spacer ring (16) connected horizontally in sequence, the fourth spacer ring (16) is connected to the sixth lens (17), and the second flexible link is mounted on the fourth spacer ring (16).

8. A microscopic objective lens for protein detection according to claim 1, characterized in that, The microscope objective further includes a first outer lens barrel (1), a second outer lens barrel (2), and a third outer lens barrel (3). The first outer lens barrel (1) and the third outer lens barrel (3) are both mounted on a first lens mount (4). A second lens mount (5) is mounted inside the third outer lens barrel (3). The second outer lens barrel (2) is connected to both the first outer lens barrel (1) and the third outer lens barrel (3). A through guiding groove corresponding to the trigger protection block is provided at one end of the third outer lens barrel (3) close to the trigger protection block, and the trigger protection block is fitted and mounted in the through guiding groove.

9. A microscopic objective lens for protein detection according to claim 1, characterized in that, The elastic element is a compression spring (18), the elastic element mounting groove is a spring groove (23), the spring groove (23) is provided at one end of the first lens mount (4) close to the second lens mount (5), the compression spring (18) is mounted in the spring groove (23), the trigger protection block is an extended strip-shaped block (20), and the extended strip-shaped block (20) is mounted at one end of the second lens mount (5) away from the first lens mount (4).

10. A lens protection method for a microscope objective lens used for protein detection, which is realized based on the microscope objective lens for protein detection according to any one of claims 1 to 9, characterized in that, After the trigger protection block comes into contact with the protein stage, the trigger protection block drives the second lens mount (5) and the second lens group to move along the elastic element mounting groove in the direction close to the first lens mount (4). At the same time, the strip-shaped guiding block (19) moves along the guiding groove (22) in the direction close to the first lens mount (4), and the elastic element is compressed to store energy. After the trigger protection block is separated from contact with the protein stage, the elastic element expands to release energy, the trigger protection block drives the second lens mount (5) and the second lens group to move along the elastic element mounting groove in the direction away from the first lens mount (4). At the same time, the strip-shaped guiding block (19) moves along the guiding groove (22) in the direction away from the first lens mount (4), and the second lens mount (5) and the second lens group return to their original positions.

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