Breeding method of colored sweet-waxy corn

Abstract

The invention provides a breeding method of colored sweet-waxy corn, and belongs to the technical field of corn breeding. The invention provides a breeding method of colored sweet-waxy corn, which comprises the following steps: by taking a pure yellow-grain waxy corn inbred line as a female parent and a double-recessive purple-grain super-sweet corn inbred line as a male parent, hybridizing the female parent and the male parent to obtain the colored sweet-waxy corn as a filial generation. By means of the breeding method, the sweet-waxy corn combination which is high in quality, good in resistance and good in commodity can be bred, the sweet and waxy characters are considered, the appearance is attractive, and the market requirement is met.

Description

Technical Field

[0001] The invention belongs to the technical field of corn breeding, and particularly relates to a breeding method for colored sweet and sticky corn. Background Art

[0002] Sweet corn originated in the Americas and carries mutant genes related to carbohydrate metabolism. According to genetic type and endosperm characteristics, sweet corn is divided into ordinary sweet corn, super sweet sweet corn (also known as fruit sweet corn) and enhanced sweet corn. Sweet corn is mainly used for fresh ears and preservation processing. Fresh corn is a special type of corn that is harvested during the milky stage and its fresh and tender ears are eaten like fruits and vegetables. It has comprehensive advantages and fashionable characteristics such as high nutritional value, good taste, high added value, good benefits, low fat and high fiber. The planting area of ​​fresh corn in my country has reached more than 20 million mu, and it is showing a trend of continuous expansion, but there are few high-quality new varieties, which restricts the development of the fresh corn industry. Therefore, the selection and breeding of new varieties of high-quality fresh corn has become an important direction of corn breeding.

[0003] The breeding of fresh corn, such as sweet corn, is generally combined with conventional breeding, where the sweet quality gene of sweet corn is introduced into materials with good genetic background, and inbred lines are selected in combination with breeding goals, resulting in that most of the fresh corn currently planted are inbred lines. Therefore, how to provide a stable and high-quality fresh corn hybrid breeding method is an urgent problem to be solved by those skilled in the art. Summary of the invention

[0004] The present invention provides a method for breeding colored sweet and sticky corns. Compared with other methods, the high-quality colored sweet and sticky corn combination bred by the method has high quality and good commercial properties, thus meeting market demand.

[0005] The invention provides a method for breeding colored sweet and sticky corn, comprising taking a pure yellow-grained sticky corn inbred line as a female parent and a double recessive purple-grained super sweet corn inbred line as a male parent, hybridizing the female parent and the male parent, and obtaining the colored sweet and sticky corn as a hybrid offspring.

[0006] In a preferred embodiment of the present invention, the breeding method of the female parent comprises: using Kennian No. 1 and N263 glutinous corn as a hybrid combination, selecting yellow-grained individual plants for self-pollination for several consecutive generations, and selecting a stable pure yellow-grain glutinous corn inbred line as the female parent.

[0007] In a preferred embodiment of the present invention, in the hybrid combination, Kennian No. 1 is the female parent and N263 glutinous corn is the male parent.

[0008] In a preferred embodiment of the present invention, the number of generations of self-pollination is 6.

[0009] In a preferred embodiment of the present invention, after 6 generations of self-pollination, the method further comprises performing quality screening and combining ability determination on the selected individual plants.

[0010] In a preferred embodiment of the present invention, the breeding method of the male parent comprises: hybridizing Ziyu Nuo No. 1 with Baitian No. 1, selecting purple kernels for planting and then backcrossing with Ziyu Nuo No. 1, selecting purple kernels for planting and then selfing for several generations, then selecting white sweet kernels for planting, and then continuously selfing for several generations to select a stable double recessive purple kernel super sweet corn inbred line as the male parent.

[0011] In a preferred embodiment of the present invention, during the hybridization, Ziyu Nuo No. 1 is used as the female parent and Baitian No. 1 is used as the male parent.

[0012] In a preferred embodiment of the present invention, the purple sweet corn kernels are selected for planting, self-pollinated for 2 generations, and then white sweet kernels are selected for planting, followed by continuous self-pollination for 4 generations.

[0013] In a preferred embodiment of the present invention, the method further comprises conducting a combining ability test after the continuous self-pollination for 4 generations.

[0014] The present invention also provides the use of the colored sweet and sticky corn bred by the breeding method in improving the yield and quality of fresh corn.

[0015] Beneficial effect: The present invention provides a breeding method for colored sweet and sticky corn, comprising using a pure yellow-grained glutinous corn inbred line as a female parent and a double recessive purple-grained super sweet corn inbred line as a male parent, and hybridizing the female parent and the male parent to obtain the colored sweet and sticky corn. The colored sweet and sticky corn bred by the breeding method of the present invention has a plant height of 220.0 cm and an ear height of 104.0 cm from seedling to harvest in the spring sowing in Shanghai about 80 days, a compact plant type, vigorous seedling growth, good uniformity, an ear length of 18.8 cm, an ear thickness of 4.8 cm, a cone-shaped ear type, 14 rows of ears, about 32 grains per row, purple, yellow, and white grains, good steaming and eating taste, sweet and sticky, a quality evaluation grade summary score of 88 points, stable variety quality, strong resistance and wide adaptability. It can be seen that the method of the present invention can cultivate a sweet and sticky corn combination with high quality, good resistance and good commercial value, which has both sweet and sticky properties and good appearance, thus meeting market demand. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 The present invention is a breeding flow chart of colored sweet and sticky corn. DETAILED DESCRIPTION

[0017] The invention provides a method for breeding colored sweet and sticky corn, comprising taking a pure yellow-grained sticky corn inbred line as a female parent and a double recessive purple-grained super sweet corn inbred line as a male parent, hybridizing the female parent and the male parent, and obtaining the colored sweet and sticky corn as a hybrid offspring.

[0018] In one embodiment of the present invention, Figure 1 The process shown is used to breed the colored sweet glutinous corn, with a pure yellow-grain glutinous corn inbred line as the female parent, and the breeding method of the female parent includes: using Kennian No. 1 and N263 glutinous corn as a hybrid combination, selecting yellow-grained individual plants for continuous self-pollination for several generations, and screening the stable pure yellow-grain glutinous corn inbred line as the female parent. In the hybrid combination, Kennian No. 1 is the female parent and N263 glutinous corn is the male parent. Kennian No. 1 described in the present invention is a glutinous corn with an approval number of Guoshenyu 2003031, light yellow grains, yellow-white grain tops, high resistance to large spot disease, head smut and corn borer, resistance to gray spot disease and Curvularia leaf spot disease, and medium resistance to stem rot. The N263 glutinous corn described in the present invention is a high-generation system resource, which has been disclosed in the article (Wu Yueqin. Breeding of a new variety of fresh glutinous corn with high yield and stable yield, Shennuo No. 8. Experimental research. 2018.6).

[0019] In one embodiment of the present invention, yellow-grained individual plants among the hybrid offspring are selected for continuous self-pollination, and the specific number of self-pollination generations is 6 generations. The present invention also includes quality screening and combining ability determination for the selected individual plants after the 6 generations of self-pollination. When the quality screening is performed in one embodiment of the present invention, the screening is mainly performed from the two aspects of yield and quality, and plants with good growth, lodging resistance, developed root system, high yield and high quality score are selected; when the combining ability is determined, the yield of the hybrid generation is mainly used as the main measurement indicator, and the stable inbred line is used for bag hybridization with the existing excellent inbred line. The offspring with high yield have high combining ability, and the offspring with low yield have low combining ability.

[0020] The present invention uses a double recessive purple grain super sweet corn inbred line as the male parent, and the breeding method of the male parent includes: hybridizing Ziyu Nuo No. 1 with Baitian No. 1, selecting purple grains for planting and backcrossing with Ziyu Nuo No. 1, selecting purple grains for planting and selfing for several generations after selecting white sweet grains for planting, and then selfing for several generations continuously, and screening a stable double recessive purple grain super sweet corn inbred line as the male parent. In a preferred embodiment of the present invention, during the hybridization, Ziyu Nuo No. 1 is used as the female parent and Baitian No. 1 is used as the male parent. The approved number of Ziyu Nuo No. 1 of the present invention is Su Shenyu 200202, which is semi-compact, with pale purple-red ears, cone-shaped ears, good glutinousness, thin grain cortex, ear length of 16.6 cm, ear thickness of 4.0 cm, and baldness of 0.7 cm; the approved number of Baitian No. 1 is Zhe Shenyu 2007002, which is white grain sweet corn. After the hybridization, the present invention selects offspring with purple grains for backcrossing with Ziyu Nuo No. 1, and selects purple sweet corn grains from the backcross offspring for planting, and then performs self-pollination for two consecutive generations, selects white sweet grains for planting, and then self-pollinates for four consecutive generations to screen a stable double recessive purple super sweet corn inbred line. The present invention also includes performing a combining ability determination after the four consecutive generations of self-pollination, and the combining ability determination mainly uses the yield of its hybrid generation as the main measurement index, and uses the stable inbred line to perform bag hybridization with the existing excellent inbred line, and the offspring with high yield have high combining ability, and the offspring with low yield have low combining ability.

[0021] The present invention also provides the use of the colored sweet and sticky corn bred by the breeding method in improving the yield and quality of fresh corn.

[0022] By using the breeding method of the present invention, the colored sweet and sticky corn combination bred in the embodiment is about 80 days from Shanghai spring sowing seedling to harvest, with a plant height of 220.0 cm, an ear height of 104.0 cm, a compact plant type, vigorous seedling growth, good uniformity, an ear length of 18.8 cm, an ear thickness of 4.8 cm, a cylindrical cone type ear, 14 rows of ears, about 32 grains per row, purple, yellow and white grains, good taste when steamed and eaten, sweet and sticky, a quality evaluation grade summary score of 88 points, stable variety quality, strong resistance, and wide adaptability.

[0023] In order to further illustrate the present invention, a breeding method for colored sweet and sticky corn provided by the present invention is described in detail below in conjunction with embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0024] Example 1

[0025] according to Figure 1 The process shown is used to breed new varieties:

[0026] (1) Female parent 120: In the spring of 2005, the combination of Kennian No. 1 and N263 glutinous corn was hybridized at the base of Shanghai Lilifeng Agricultural Science and Technology Co., Ltd., and excellent individual plants with yellow grains (about 105 days from seedling emergence to grain maturity, ear length greater than 17 cm, 12 to 14 ear rows, yellow grains, and thin fruit skin) were selected. They were self-pollinated for six consecutive generations, and the pure yellow-grain glutinous corn inbred line that was stabilized in 2009 was used as the female parent.

[0027] (2) Male parent 126: In 2009, Ziyunuo 1 and Baitian 1 were hybridized at the base of Shanghai Lilifeng Agricultural Science and Technology Co., Ltd. The purple kernels were selected for planting and then backcrossed with Zinuo 1. The purple kernels were selected for planting and then self-pollinated twice. Then white kernels were selected for planting. After four consecutive generations of self-pollination, the double recessive purple kernel super sweet corn inbred line was stabilized in 2014 as the male parent.

[0028] (3) Crossbreeding: Crossbreeding was carried out at the Jinshan base of Shanghai Seed Group in the spring of 2016. Variety observation tests were conducted in the fall of 2016 (different combinations were prepared using different inbred lines, and then the ones with a quality score greater than 85 were selected, and the yield was ranked from high to low). A variety comparison test was conducted in the spring of 2017 (SHN7, which was evaluated based on yield and quality, mainly recording the growth period and plant type, measuring plant height, ear height, ear length, ear diameter, number of kernels per ear, number of rows per ear, and number of kernels per row, and determining yield and quality scores). The selected colored sweet and glutinous corn combination was seeded in Shanghai in the spring. Around 80 days after harvest, the plant height was 220.0 cm, the ear height was 104.0 cm, the plant shape was compact, the seedlings grew vigorously, the uniformity was good, the ear length was 18.8 cm, the ear thickness was 4.8 cm, the ear shape was cylindrical-conical, there were 14 rows of ears, and there were about 32 grains in a row. The grain color was purple, yellow and white, and it tasted good when steamed and eaten, sweet and glutinous. The quality evaluation grade summary score was 88 points. The variety had stable quality, strong resistance and wide adaptability (after planting this combination in Jiading, Jinshan and Songjiang, the yield was stable, all above 800 kg, higher than the control).

[0029] Table 1 Product comparison test data

[0030]

[0031]

[0032] Table 2 Quality evaluation table

[0033]

[0034] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A method for breeding colored sweet and sticky corn, characterized in that: The method comprises taking a pure yellow-grain glutinous corn inbred line as a female parent and a double recessive purple-grain super sweet corn inbred line as a male parent, and after hybridizing the female parent and the male parent, the hybrid offspring is the colored sweet glutinous corn.

2. The breeding method according to claim 1, characterized in that: The breeding method of the female parent comprises: using Kennian No. 1 and N263 glutinous corn as a hybrid combination, selecting yellow-grained individual plants for self-pollination for several consecutive generations, and selecting a stable pure yellow-grain glutinous corn inbred line as the female parent.

3. The breeding method according to claim 2, characterized in that: In the hybrid combination, Kennian No. 1 is used as the female parent, and N263 glutinous corn is used as the male parent.

4. The breeding method according to claim 2, characterized in that: The number of self-pollination generations is 6.

5. The breeding method according to claim 4, characterized in that: After 6 generations of self-pollination, the selected plants are screened for quality and tested for combining ability.

6. The breeding method according to claim 1, characterized in that: The breeding method of the male parent comprises: hybridizing Ziyu Nuo No. 1 with Baitian No. 1, selecting purple kernels for planting and then backcrossing with Ziyu Nuo No. 1, selecting purple kernels for planting and then self-pollinating for several generations, then selecting white sweet kernels for planting, and then continuously self-pollinating for several generations to select a stable double recessive purple kernel super sweet corn inbred line as the male parent.

7. The breeding method according to claim 6, characterized in that: During the hybridization, Ziyunuo No. 1 is used as the female parent and Baitian No. 1 is used as the male parent.

8. The breeding method according to claim 6, characterized in that: It includes selecting purple sweet corn kernels for planting, self-pollinating for 2 generations, selecting white sweet kernels for planting, and then self-pollinating for 4 consecutive generations.

9. The breeding method according to claim 8, characterized in that: After the continuous self-pollination for 4 generations, the combining ability test is also performed.

10. Use of colored sweet and sticky corn bred by the breeding method according to any one of claims 1 to 9 in improving the yield and quality of fresh corn.

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