Feed capable of increasing subcutaneous fat content of Marigans geese as well as preparation method and application of feed

Abstract

The invention provides a feed capable of increasing the subcutaneous fat content of Manggang geese as well as a preparation method and application of the feed, and belongs to the technical field of animal feeds. The feed is prepared by mixing and grinding the corn, the wheat, the bean cakes and the haws into powder, and mixing and granulating the dried fish powder, the carrot powder, the kelp powder, the edible salt, the premix, the beta-glucanase microcapsules and water. The feed prepared by the invention adopts a proportion of high energy and low protein to maintain a proper proportion of energy and protein so as to meet the growth requirements of the Manchuan geese. The beta-glucanase microcapsule provided by the invention can protect beta-glucanase from being damaged by gastric acid, improve the digestibility of chyme and the energy utilization rate of the feed, and further promote the deposition of subcutaneous fat of the Manggang geese.

Description

Technical Field

[0001] The invention belongs to the technical field of animal feed, and particularly relates to a feed for improving the subcutaneous fat content of Magang geese, and a preparation method and application thereof. Background Art

[0002] Magang goose is a famous meat goose breed in China, known for its delicious meat and rich skin fat. In southern China, especially in Guangdong Province, Magang goose is deeply loved by consumers for its unique flavor. Subcutaneous fat is not only one of the important indicators for evaluating the quality of goose meat, but also greatly affects the taste and nutritional value of the meat. The right amount of subcutaneous fat can make the meat more tender and juicy, improve the overall eating experience, and thus gain higher recognition and price advantages in the market. With the increasing demand of consumers for high-quality goose meat, how to increase the subcutaneous fat content of Magang geese through scientific methods has become an urgent problem for farmers and researchers. Traditional breeding methods often rely on experience and intuition, lack of systematic scientific basis, and it is difficult to achieve efficient and stable fat-increasing effects. In addition, the growing demand for high-quality goose meat in the market has also prompted breeders to constantly seek new technologies and methods to improve product quality.

[0003] Developing a high-energy, low-protein feed formula is one of the effective ways to achieve this goal. High-energy, low-protein feed can meet the energy needs of geese while avoiding unnecessary metabolic burden caused by excessive protein intake, thereby achieving efficient fat gain. Specifically, high-energy feed can provide sufficient calories, so that geese have more surplus energy for fat synthesis and deposition; while low-protein feed can reduce the metabolic burden of nitrogen in the goose body and reduce the production of ammonia, which is conducive to maintaining the health of the goose.

[0004] After geese eat feed containing barley, the β-glucan contained in it will increase the viscosity of the chyme. This increase in viscosity will undoubtedly hinder the interaction between endogenous digestive enzymes and various nutrients, resulting in a decrease in digestibility, affecting the normal growth of the body, while increasing the excretion of feces and aggravating environmental pollution. When β-glucanase is added to feed containing barley, the situation will change significantly. β-glucan can degrade β-glucan into low-molecular sugars, destroy the structure of plant cell walls, and release nutrients. The degraded β-glucan loses its hydrophilicity and viscosity, and will not swell and adhere in the intestines of geese, significantly improving the digestive function of geese, promoting the absorption of nutrients, effectively improving the body's utilization efficiency of feed, creating favorable conditions for the deposition of fat, and helping to improve the breeding benefits and product quality of geese. However, the pH value of different parts of the goose's digestive tract varies greatly, from the strongly acidic environment of the stomach to the neutral or weakly alkaline environment of the small intestine. The optimum pH of most β-glucanases is usually in the neutral or slightly acidic range, which means that the activity of the enzyme will be significantly reduced in the low pH environment of the stomach.

[0005] Therefore, it is an urgent problem to be solved in the art to prepare a feed which can not only protect β-glucanase from the adverse effects of gastric acid and improve the digestive function of geese, but also effectively increase the subcutaneous fat content of Magang geese. Summary of the invention

[0006] In view of the problems existing in the prior art, the present invention provides a feed for increasing the subcutaneous fat content of Magang geese, a preparation method thereof and an application thereof. The β-glucanase microcapsules of the present invention can protect the β-glucanase from being destroyed by gastric acid, improve the digestibility of chyme and the energy utilization of feed, and further promote the deposition of subcutaneous fat of Magang geese.

[0007] To achieve the above object, the present invention adopts the following technical solution:

[0008] A method for preparing a feed for increasing the subcutaneous fat content of Magang geese, the preparation method comprising the following steps:

[0009] Corn, wheat, bean cake and hawthorn are mixed and ground into powder, and dried fish powder, carrot powder, kelp powder, edible salt, premix, beta-glucanase microcapsule and water are mixed and granulated to prepare the feed.

[0010] Preferably, the premix contains calcium hydrogen phosphate, zinc sulfate monohydrate, ferrous sulfate monohydrate, copper sulfate pentahydrate, manganese sulfate monohydrate, vitamin D3, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B 12, L-lysine hydrochloride, DL-methionine and carrier weight ratio is 60:35:54:6.5:53:0.4:2:0.5:0.6:0.6:0.9:0.03:0.5:0.5:100.

[0011] Preferably, the carrier is wheat bran.

[0012] Preferably, the method for preparing the β-glucanase microcapsules comprises the following steps:

[0013] Disperse carboxymethyl chitosan in 5% acetic acid solution and stir for 1-2 hours, add initiator and allyl cinnamate in an inert gas protection environment, heat to 60-70°C and stir for reaction for 2-4 hours to obtain a copolymer;

[0014] The copolymer is dispersed in a 1% acetic acid solution to obtain a dispersion, β-glucanase is dissolved in a PBS buffer solution to obtain an enzyme solution, and the enzyme solution is added to the dispersion to obtain an aqueous phase; an emulsifier is added to liquid paraffin and stirred at 800-1000 r / min for 0.5-1h to obtain an oil phase, the aqueous phase is added to the oil phase, and emulsified at a speed of 1000-1200 r / min for 10-30min, a cross-linking agent is added after the emulsification is completed, and the β-glucanase microcapsules are cured at 400-60°C at 400-600 r / min for 2-3h.

[0015] Preferably, the β-glucanase comprises β-1,3-glucanase or β-1,4-glucanase.

[0016] Preferably, the weight ratio of the carboxymethyl chitosan to allyl cinnamate is 2-3:2.5-4.

[0017] Preferably, the weight ratio of the initiator to carboxymethyl chitosan is 0.3-0.5:1.

[0018] Preferably, the initiator is a mixture of ammonium persulfate and sodium bisulfite.

[0019] Preferably, the weight ratio of ammonium sulfate to sodium bisulfite in the superinitiator is 1:1.

[0020] Preferably, the inert gas is nitrogen.

[0021] Preferably, the weight ratio of the copolymer to β-glucanase is 1:0.02-0.04.

[0022] Preferably, the weight ratio of the oil phase to the water phase is 15-20:1.

[0023] Preferably, the conditions of the PBS buffer solution include a pH value of 7.5 and a molar concentration of 30 mM.

[0024] Preferably, the emulsifier is Span-80.

[0025] Preferably, the weight ratio of the cross-linking agent to the mixed phase is 0.01-0.02:1.

[0026] Preferably, the cross-linking agent is glutaraldehyde.

[0027] Preferably, in the emulsification and spheronization stage, an amphiphilic graft copolymer is prepared by grafting allyl cinnamate onto the amino groups of carboxymethyl chitosan molecular chains, the hydrophilic groups in the amphiphilic graft copolymer make the copolymer tend to be soluble in the aqueous phase, and the lipophilic groups make the copolymer tend to be soluble in the oil phase, the two groups work together to reduce its surface tension in the solution, the copolymer self-assembles into micelles in the aqueous solution, resulting in the aqueous phase containing β-glucanase and the amphiphilic graft copolymer being emulsified and dispersed into small and uniformly dispersed emulsion droplets suspended in the oil phase, thereby obtaining a stable W / O emulsion; in the cross-linking and curing spheronization stage, the aldehyde groups on the glutaraldehyde molecular chains react with the hydroxyl groups on the carboxymethyl chitosan molecular chains to undergo an aldol condensation reaction, so that the carboxymethyl chitosan molecular chains are cross-linked to form a network structure, the microcapsules are cured, and the β-glucanase is fixed in the microcapsules.

[0028] Preferably, the presence of benzene ring in allyl cinnamate improves the heat resistance of the amphiphilic graft copolymer, and in the subsequent cross-linking and curing reaction process, when a higher reaction temperature is required, it can effectively protect the β-glucanase from being damaged by the high temperature environment.

[0029] Preferably, the powder is obtained by screening through a 100-200 mesh sieve.

[0030] The mixing and granulation conditions include a mixing speed of 100 to 200 r / min and a mixing time of 5 to 15 min.

[0031] Preferably, the weight ratio of the wheat, corn, bean cake, hawthorn, dried fish powder, carrot powder, kelp powder, edible salt, premix, β-glucanase microcapsules and water is 30-40:20-30:5-15:2-6:3-5:2-4:1-2:0.5-1.5:3-5:2-3:10-30.

[0032] A feed prepared by a method for preparing a feed for increasing the subcutaneous fat content of Magang geese.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] (1) The present invention prepares β-glucan microcapsules by an emulsification cross-linking method. In the acidic environment of the stomach, the protonation of the carboxyl groups in the microcapsules weakens the electrostatic repulsion in the system, resulting in the contraction of the molecular chains and enhancing the compactness and stability of the microcapsules; however, in the neutral or alkaline environment of the small intestine, the carboxyl groups are deprotonated, the microcapsule skeleton relaxes and swells and ruptures to release β-glucanase, thereby improving the digestive function of geese.

[0035] (2) The feed prepared by the present invention adopts a high energy and low protein ratio to maintain an appropriate energy and protein ratio to meet the growth needs of Magang geese. Excessive intake of protein will cause it to be converted into amino acids instead of being stored in the form of fat. Increasing the ratio of carbohydrates and fat appropriately can effectively increase the subcutaneous fat content. DETAILED DESCRIPTION

[0036] The technical solution of the present invention will be clearly and completely described below through the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present invention.

[0037] Unless otherwise specified, the raw materials and reagents used in the present invention are commercially available or can be prepared by known methods.

[0038] Preparation Example 1:

[0039] The preparation of the premix comprises the following steps:

[0040] 60 parts by weight of calcium hydrogen phosphate, 35 parts by weight of zinc sulfate monohydrate, 54 parts by weight of ferrous sulfate monohydrate, 6.5 parts by weight of copper sulfate pentahydrate, 53 parts by weight of manganese sulfate monohydrate, 0.4 parts by weight of vitamin D3, 2 parts by weight of vitamin E, 0.5 parts by weight of vitamin K, 0.6 parts by weight of vitamin B1, 0.6 parts by weight of vitamin B2, 0.9 parts by weight of vitamin B6, 0.03 parts by weight of vitamin B 12 , 1.5 parts by weight of vitamin B, 0.5 parts by weight of L-lysine hydrochloride, 0.5 parts by weight of DL-methionine and 100 parts by weight of wheat bran are mixed in a mixer at a rotation speed of 30 rpm for 30 minutes to prepare a premix.

[0041] Preparation Example 2:

[0042] The preparation of β-glucanase microcapsules comprises the following steps:

[0043] 2 parts by weight of carboxymethyl chitosan were dispersed in 50 parts by weight of 5% acetic acid solution and stirred for 1 hour, 0.3 parts by weight of ammonium persulfate, 0.3 parts by weight of a mixed solution of sodium bisulfite and 2.5 parts by weight of allyl cinnamate were added in a nitrogen atmosphere, the mixture was heated to 60°C and stirred for 2 hours to obtain a copolymer; 1 part by weight of the above copolymer was dispersed in 80 parts by weight of 1% acetic acid solution to obtain a dispersion, 0.02 parts by weight of β-1,3-glucanase was dissolved in 1 part by weight of PBS buffer solution (pH 7.5, 30 mM) Obtain enzyme solution, add the enzyme solution to the above dispersion to obtain an aqueous phase; add 1 weight part of Span-80 to 40 weight parts of liquid paraffin and stir at 800 r / min for 0.5 h to obtain an oil phase; add 1 weight part of the aqueous phase to 15 weight parts of the oil phase to obtain a mixed phase; emulsify at room temperature at a speed of 1000 r / min for 10 min; after the emulsification is completed, add 0.16 weight part of glutaraldehyde; cure at 400 r / min for 3 h in an environment of 40°C; after the curing is completed, wash, filter, and dry to obtain β-glucanase microcapsules.

[0044] Preparation Example 3:

[0045] The preparation of β-glucanase microcapsules comprises the following steps:

[0046] 2.5 parts by weight of carboxymethyl chitosan were dispersed in 60 parts by weight of 5% acetic acid solution and stirred for 1.5 hours, 0.5 parts by weight of ammonium persulfate, 0.5 parts by weight of sodium bisulfite and 3 parts by weight of allyl cinnamate were added in a nitrogen atmosphere, the temperature was raised to 70°C and stirred for reaction for 2.5 hours to obtain a copolymer; 1 part by weight of the above copolymer was dispersed in 90 parts by weight of 1% acetic acid solution to obtain a dispersion, 0.03 parts by weight of β-1,3-glucanase was dissolved in 1 part by weight of PBS buffer solution (pH 7.5, 30 mM) to obtain Enzyme solution, adding the enzyme solution to the above dispersion to obtain an aqueous phase; adding 1.5 weight parts of Span-80 to 50 weight parts of liquid paraffin and stirring at 1000r / min for 1h to obtain an oil phase, adding 1 weight part of the aqueous phase to 16 weight parts of the oil phase to obtain a mixed phase, emulsifying at room temperature at a speed of 1100r / min for 20min, adding 0.23 weight part of glutaraldehyde after the emulsification, curing at 50°C and 500r / min for 2.5h, washing, filtering and drying after the curing to obtain β-glucanase microcapsules.

[0047] Preparation Example 4:

[0048] The preparation of β-glucanase microcapsules comprises the following steps:

[0049] 3 parts by weight of carboxymethyl chitosan were dispersed in 100 parts by weight of 5% acetic acid solution and stirred for 2 hours, 0.75 parts by weight of ammonium persulfate, 0.75 parts by weight of sodium bisulfite and 4 parts by weight of allyl cinnamate were added in a nitrogen atmosphere, the mixture was heated to 65°C and stirred for reaction for 2.4 hours to obtain a copolymer; 1 part by weight of the above copolymer was dispersed in 100 parts by weight of 1% acetic acid solution to obtain a dispersion, 0.03 parts by weight of β-1,4-glucanase was dissolved in 1 part by weight of PBS buffer solution (pH 7.5, 30 mM) to obtain a copolymer. The enzyme solution is added to the above dispersion to obtain an aqueous phase; 1.5 parts by weight of Span-80 is added to 40 parts by weight of liquid paraffin and stirred at 900 r / min for 0.6 h to obtain an oil phase; 1 part by weight of the aqueous phase is added to 18 parts by weight of the oil phase to obtain a mixed phase; the mixture is emulsified at a speed of 1200 r / min for 15 min at room temperature; after the emulsification is completed, 0.3 parts by weight of glutaraldehyde is added; the mixture is cured at 400 r / min for 2 h at 60°C; after the curing is completed, the β-glucanase microcapsules are washed, filtered, and dried.

[0050] Preparation Example 5:

[0051] 3 parts by weight of carboxymethyl chitosan were dispersed in 80 parts by weight of 5% acetic acid solution and stirred for 2 hours, 0.45 parts by weight of ammonium persulfate, 0.45 parts by weight of sodium bisulfite and 4 parts by weight of allyl cinnamate were added in a nitrogen atmosphere, the temperature was raised to 70°C and stirred for 4 hours to obtain a copolymer; 1 part by weight of the above copolymer was dispersed in 100 parts by weight of 1% acetic acid solution to obtain a dispersion, 0.04 parts by weight of β-1,4-glucanase was dissolved in 1 part by weight of PBS buffer solution (pH 7.5, 30 mM) Obtain enzyme solution, add the enzyme solution to the above dispersion to obtain an aqueous phase; add 2 parts by weight of Span-80 to 80 parts by weight of liquid paraffin and stir at 1000 r / min for 1 hour to obtain an oil phase; add 1 part by weight of the aqueous phase to 20 parts by weight of the oil phase to obtain a mixed phase; emulsify at room temperature at a speed of 1200 r / min for 30 minutes; after the emulsification is completed, add 4.2 parts by weight of glutaraldehyde; solidify at 600 r / min for 2 at 50°C; after the solidification is completed, wash, filter, and dry to obtain β-glucanase microcapsules.

[0052] Preparation Example 6:

[0053] 1 part by weight of carboxymethyl chitosan is dispersed in 100 parts by weight of 1% acetic acid solution to obtain a dispersion, 0.04 parts by weight of β-1,4-glucanase is dissolved in 1 part by weight of PBS buffer solution (pH7.5, 30mM) to obtain an enzyme solution, and the enzyme solution is added to the above dispersion to obtain an aqueous phase; 2 parts by weight of Span-80 are added to 80 parts by weight of liquid paraffin and stirred at 1000r / min for 1h to obtain an oil phase, 1 part by weight of the aqueous phase is added to 20 parts by weight of the oil phase to obtain a mixed phase, and emulsified at a speed of 1200r / min for 30min at room temperature, and after the emulsification is completed, 4.2 parts by weight of glutaraldehyde is added, and the mixture is cured at 600r / min for 2h in a 50°C environment, and after the curing is completed, the mixture is washed, filtered, and dried to obtain β-glucanase microcapsules.

[0054] Embodiment 1:

[0055] A preparation method for a feed for increasing the subcutaneous fat content of Magang geese comprises the following steps:

[0056] 30 parts by weight of wheat, 20 parts by weight of corn, 5 parts by weight of bean cake and 2 parts by weight of hawthorn were mixed and ground, and the powder was sieved with a 200-mesh sieve. 3 parts by weight of dried fish powder, 2 parts by weight of carrot powder, 1 part by weight of kelp powder, 0.5 parts by weight of edible salt, 3 parts by weight of the premix of Preparation Example 1, 2 parts by weight of β-glucanase microcapsules prepared in Preparation Example 2 and 10 parts by weight of water were mixed at a speed of 100 r / min for 5 minutes and granulated to prepare feed.

[0057] Embodiment 2:

[0058] A preparation method for a feed for increasing the subcutaneous fat content of Magang geese comprises the following steps:

[0059] 33 parts by weight of wheat, 30 parts by weight of corn, 10 parts by weight of bean cake and 6 parts by weight of hawthorn were mixed and ground, and the powder was sieved with a 100-mesh sieve. 4 parts by weight of dried fish powder, 3 parts by weight of carrot powder, 1.5 parts by weight of kelp powder, 1 part by weight of edible salt, 4 parts by weight of the premix of Preparation Example 1, 2.5 parts by weight of β-glucanase microcapsules prepared in Preparation Example 3 and 20 parts by weight of water were mixed at a speed of 150 r / min for 10 minutes and granulated to prepare feed.

[0060] Embodiment 3:

[0061] A preparation method for a feed for increasing the subcutaneous fat content of Magang geese comprises the following steps:

[0062] 36 parts by weight of wheat, 25 parts by weight of corn, 15 parts by weight of bean cake and 4 parts by weight of hawthorn were mixed and ground, and the powder was sieved with a 150-mesh sieve. 5 parts by weight of dried fish powder, 4 parts by weight of carrot powder, 2 parts by weight of kelp powder, 1.5 parts by weight of edible salt, 5 parts by weight of the premix of Preparation Example 1, 3 parts by weight of β-glucanase microcapsules prepared in Preparation Example 4 and 30 parts by weight of water were mixed at a speed of 150 r / min for 15 minutes and granulated to prepare feed.

[0063] Embodiment 4:

[0064] A preparation method for a feed for increasing the subcutaneous fat content of Magang geese comprises the following steps:

[0065] 40 parts by weight of wheat, 20 parts by weight of corn, 10 parts by weight of bean cake and 2 parts by weight of hawthorn were mixed and ground, and the powder was sieved with a 100-mesh sieve. 5 parts by weight of dried fish powder, 4 parts by weight of carrot powder, 1 part by weight of kelp powder, 1.5 parts by weight of edible salt, 4 parts by weight of the premix of Preparation Example 1, 4 parts by weight of β-glucanase microcapsules prepared in Preparation Example 5 and 20 parts by weight of water were mixed at a speed of 200 r / min for 15 minutes and granulated to prepare feed.

[0066] Comparative Example 1:

[0067] A preparation method for a feed for increasing the subcutaneous fat content of Magang geese comprises the following steps:

[0068] 40 parts by weight of wheat, 20 parts by weight of corn, 10 parts by weight of bean cake and 2 parts by weight of hawthorn were mixed and ground, and the powder was sieved with a 100-mesh sieve. 5 parts by weight of dried fish powder, 4 parts by weight of carrot powder, 1 part by weight of kelp powder, 1.5 parts by weight of edible salt, 4 parts by weight of the premix of Preparation Example 1, 4 parts by weight of β-glucanase and 20 parts by weight of water were mixed at a speed of 200 r / min for 15 minutes and granulated to prepare feed.

[0069] Comparative Example 2:

[0070] A preparation method for a feed for increasing the subcutaneous fat content of Magang geese comprises the following steps:

[0071] 40 parts by weight of wheat, 20 parts by weight of corn, 10 parts by weight of bean cake and 2 parts by weight of hawthorn were mixed and ground, and the powder was sieved with a 100-mesh sieve. 5 parts by weight of dried fish powder, 4 parts by weight of carrot powder, 1 part by weight of kelp powder, 1.5 parts by weight of edible salt, 4 parts by weight of the premix of Preparation Example 1, 4 parts by weight of β-glucanase microcapsules prepared in Preparation Example 6 and 20 parts by weight of water were mixed at a speed of 200 r / min for 15 minutes and granulated to prepare feed.

[0072] Comparative Example 3:

[0073] A preparation method for a feed for increasing the subcutaneous fat content of Magang geese comprises the following steps:

[0074] 40 parts by weight of wheat, 20 parts by weight of corn, 20 parts by weight of bean cake and 2 parts by weight of hawthorn were mixed and ground, and the powder was sieved with a 100-mesh sieve. 10 parts by weight of dried fish powder, 4 parts by weight of carrot powder, 1 part by weight of kelp powder, 1.5 parts by weight of edible salt, 4 parts by weight of the premix of Preparation Example 1, 4 parts by weight of β-glucanase microcapsules prepared in Preparation Example 5 and 20 parts by weight of water were mixed at a speed of 200 r / min for 15 minutes and granulated to prepare feed.

[0075] Comparative Example 4:

[0076] A preparation method for a feed for increasing the subcutaneous fat content of Magang geese comprises the following steps:

[0077] 40 parts by weight of wheat, 20 parts by weight of corn, 15 parts by weight of bean cake and 2 parts by weight of hawthorn were mixed and ground, and the powder was sieved with a 100-mesh sieve. 10 parts by weight of dried fish powder, 4 parts by weight of carrot powder, 1 part by weight of kelp powder, 1.5 parts by weight of edible salt, 4 parts by weight of the premix of Preparation Example 1, 4 parts by weight of β-glucanase microcapsules prepared in Preparation Example 5 and 20 parts by weight of water were mixed at a speed of 200 r / min for 15 minutes and granulated to prepare feed.

[0078] Comparative Example 5:

[0079] A preparation method for a feed for increasing the subcutaneous fat content of Magang geese comprises the following steps:

[0080] 40 parts by weight of wheat, 20 parts by weight of corn, 10 parts by weight of bean cake and 2 parts by weight of hawthorn were mixed and ground, and the powder was sieved with a 100-mesh sieve. 5 parts by weight of dried fish powder, 4 parts by weight of carrot powder, 1 part by weight of kelp powder, 1.5 parts by weight of edible salt, 4 parts by weight of the premix of Preparation Example 1, 4 parts by weight of β-glucanase microcapsules prepared in Preparation Example 5 and 20 parts by weight of water were mixed at a speed of 200 r / min for 15 minutes and granulated to prepare feed.

[0081] Application examples:

[0082] The feeds obtained in Examples 1 to 4 were compared with the feeds obtained in Comparative Examples 1 to 5. 180 30-day-old Magang geese with normal growth and healthy body condition were selected and divided into 9 groups, with 20 geese in each group. The 9 groups of Magang geese were fed with the feeds obtained in Examples 1 to 4 and the feeds obtained in Comparative Examples 1 to 5, respectively. The test period was 40 days.

[0083] 1. Growth performance measurement

[0084] After the experimental period, the Magang geese were weighed on an empty stomach, and the incidence, feed intake and weight gain were recorded; finally, the average daily feed intake, average daily weight gain and feed-to-weight ratio were calculated.

[0085] Table 1. Production performance test results

[0086]

[0087]

[0088] The experimental results are shown in Table 1. The high-energy, low-protein feeds prepared in Examples 1 to 4 of the present invention are superior to the feeds prepared in Comparative Examples 1 to 5 in terms of feed-to-weight ratio and morbidity rate, especially in terms of feed-to-weight ratio and morbidity rate. The average daily weight gain of the feed prepared by the present invention is significantly higher than that of the feed prepared in Comparative Example 3, indicating that Magang geese do not need too much protein, and too much protein will affect the weight gain of Magang geese. The feed-to-weight ratio is also the lowest for the feed prepared by the present invention, which is significantly lower than the feed prepared in Comparative Example 5, which also shows that energy is an important factor affecting the feed-to-weight ratio.

[0089] 2. Slaughter performance

[0090] The thickness of subcutaneous fat of Magang geese was measured, and the sebum rate of Magang geese was calculated according to NY / T 823-2020 “Terminology and Measurement Calculation Methods for Poultry Production Performance”. The results are shown in Table 2.

[0091] Table 2. Slaughter performance test results

[0092]

[0093]

[0094] As shown in Table 2, the sebum rate and subcutaneous fat thickness of the Magang geese fed with the feed prepared in Examples 1 to 4 were higher than those of the Magang geese fed with the feed prepared in Comparative Examples 1 to 5. This indicates that the β-glucanase microcapsules significantly enhanced the digestive function of the Magang geese, and the high-energy and low-protein feed ratio also promoted the accumulation of subcutaneous fat in the Magang geese.

[0095] The above-described embodiments provide a detailed description of the technical solutions and beneficial effects of the present invention. It should be understood that the above description is only a specific embodiment of the present invention and is not intended to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected.

Claims

1. A method for preparing a feed for increasing the subcutaneous fat content of Magang geese, characterized in that: The preparation method comprises the following steps: Corn, wheat, bean cake and hawthorn are mixed and ground into powder, and dried fish powder, carrot powder, kelp powder, edible salt, premix, beta-glucanase microcapsule and water are mixed and granulated to prepare the feed.

2. The method for preparing a feed for increasing the subcutaneous fat content of Magang geese according to claim 1, characterized in that: The premix contains calcium hydrogen phosphate, zinc sulfate monohydrate, ferrous sulfate monohydrate, copper sulfate pentahydrate, manganese sulfate monohydrate, vitamin D3, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B 12 , L-lysine hydrochloride, DL-methionine and carrier in a weight ratio of 60:35:54:6.5:53:0.4: 2：0.5：0.6：0.6：0.9：0.03：0.5：0.5：100。 3. The method for preparing a feed for increasing the subcutaneous fat content of Magang geese according to claim 1, characterized in that: The preparation method of the β-glucanase microcapsules comprises the following steps: The carboxymethyl chitosan is dispersed in a 5% acetic acid solution and stirred for 1 to 2 hours, and then an initiator and allyl cinnamate are added, and the temperature is raised to 60 to 70°C and stirred for reaction for 2 to 4 hours to obtain a copolymer; the above copolymer is dispersed in a 1% acetic acid solution to obtain a dispersion, β-glucanase is dissolved in a PBS buffer solution to obtain an enzyme solution, and the enzyme solution is added to the above dispersion to obtain an aqueous phase; an emulsifier is added to liquid paraffin and stirred at 800 to 1000 r / min for 0.5 to 1 hour to obtain an oil phase, the aqueous phase is added to the oil phase, and emulsified at a speed of 1000 to 1200 r / min for 10 to 30 minutes, and a cross-linking agent is added after the emulsification is completed, and the β-glucanase microcapsules are cured in an environment of 40 to 60°C for 2 to 3 hours to obtain the β-glucanase microcapsules.

4. The method for preparing a feed for increasing the subcutaneous fat content of Magang geese according to claim 3, characterized in that: The β-glucanase includes β-1,3-glucanase or β-1,4-glucanase.

5. The method for preparing a feed for increasing the subcutaneous fat content of Magang geese according to claim 3, characterized in that: The weight ratio of the carboxymethyl chitosan to allyl cinnamate is 2-3:2.5-4.

6. The method for preparing a feed for increasing the subcutaneous fat content of Magang geese according to claim 3, characterized in that: The weight ratio of the copolymer to the beta-glucanase is 1:0.02-0.

04.

7. The method for preparing a feed for increasing the subcutaneous fat content of Magang geese according to claim 1, characterized in that: The mixing and granulation conditions include a mixing speed of 100 to 200 r / min and a mixing time of 5 to 15 min.

8. The method for preparing a feed for increasing the subcutaneous fat content of Magang geese according to claim 1, characterized in that: The weight ratio of the wheat, corn, bean cake, hawthorn, dried fish powder, carrot powder, kelp powder, edible salt, premix, beta-glucanase microcapsule and water is 30-40:20-30:5-15:2-6:3-5:2-4:1-2:0.5-1.5:3-5:2-3:10-30.

9. A feed prepared by the method for preparing a feed for increasing the subcutaneous fat content of Magang geese according to any one of claims 1 to 8.

10. Use of the feed according to claim 9 for increasing the subcutaneous fat content of Magang geese.

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