Thermal spring water composition for relieving itching and soothing skin and preparation method thereof

Abstract

The invention discloses a thermal spring water composition for relieving itching and soothing skin and a preparation method of the thermal spring water composition, and belongs to the technical field of itching relieving and soothing. Chitosan has good film-forming property, after chitosan, mung bean protein and the like form a compound, after the compound is smeared on the surface of skin, a layer of relatively stable protective film with certain air permeability can be constructed, skin allergy and inflammatory reaction can be reduced, a relatively wet microenvironment is formed on the surface of the skin, and the skin care effect is improved. The moisture loss of the skin is reduced, and the moisture balance of the skin is favorably kept, so that uncomfortable symptoms such as dry and tight skin are relieved, and the skin is further promoted to be relieved; after the centella asiatica polysaccharide is subjected to carboxymethylation modification, the water solubility and the biological activity of the centella asiatica polysaccharide are remarkably improved, and the carboxymethylated centella asiatica polysaccharide and the mung bean protein-chitosan compound have a synergistic effect, so that the barrier function of skin can be enhanced, the metabolism of skin cells can be regulated, and the skin can be effectively protected. The repair and regeneration of damaged skin cells are promoted.

Description

Technical Field

[0001] The invention belongs to the technical field of relieving itching and soothing, and in particular relates to a hot spring water composition for relieving itching and soothing the skin and a preparation method thereof. Background Art

[0002] In today's society, as living standards continue to rise, skin care has become a focus area that has attracted much attention. Skin aging problems, such as sagging and wrinkles, have become increasingly important, and people are eager to find effective solutions.

[0003] Skin moisturizing is the cornerstone of skin care. Proper moisturizing care can delay the aging process of normal skin to a certain extent. However, the core cause of skin aging lies in the gradual decline and even apoptosis of cell functions. Once problems occur at the cell level, the aging trend of the skin will inevitably appear. Therefore, effectively improving the proliferation and repair ability of cells is of great significance to fundamentally delay skin aging.

[0004] Natural hot spring water has a long history of application in the fields of beauty, skin care and health care. It is rich in a variety of trace elements, minerals, mineral salts and proteins that are beneficial to the skin. It has the effects of anti-oxidation, scavenging free radicals, improving skin aging, making the skin soft and smooth, and reducing wrinkles. Unfortunately, the presence of a large number of ions in hot spring water can easily cause emulsion demulsification in the cosmetic preparation process, resulting in instability of the emulsification system, making it difficult for the minerals in the hot spring water to be fully absorbed by the skin. This defect not only weakens the efficacy of hot spring water skin care products, but also makes people with sensitive skin stay away from it, limiting the wider application and promotion of natural hot spring water in the field of skin care. In addition, although some existing skin care products can provide certain moisturizing or antioxidant functions, they have failed to form an effective synergy in enhancing the stability of the skin barrier, stimulating cell proliferation and repair to restore skin elasticity and firmness, and comprehensively resisting external stimuli and free radical damage, making it difficult to meet people's needs for all-round skin care and solving various skin problems. Summary of the invention

[0005] In view of the above situation, in order to overcome the defects of the prior art, the present invention can penetrate deeply into the skin to improve various problems through the synergistic effect of various ingredients, build a protective barrier, and achieve comprehensive skin care effects such as antipruritic, soothing, and anti-aging.

[0006] In order to achieve the above-mentioned purpose, the following technical scheme is adopted: on the one hand, the present invention provides a hot spring water composition for relieving itching and soothing the skin, comprising the following components in parts by weight: 1-4 parts of polypeptide, 0.1-0.25 parts of thickener, 0.2-0.6 parts of pyrroloquinoline quinone disodium salt, 0.1-0.5 parts of PCA zinc, 0.1-0.5 parts of calcium pantothenate, 0.3-0.5 parts of bisabolol, 0.2-0.5 parts of paeonol, 0.5-1.5 parts of antipruritic complex, 0.5-2 parts of Zanthoxylum bungeanum fruit extract, 0.1-3 parts of Perilla leaf extract, 0.1-3 parts of grape seed extract, 15-20 parts of hot spring water, 0.5-2 parts of emulsifier, 5-15 parts of moisturizer, and 50-80 parts of water.

[0007] Furthermore, the antipruritic complex is prepared by the following steps:

[0008] S1. Add the Centella asiatica extract to deionized water at a mass ratio of 1-2%, treat in a constant temperature water bath at 40-50°C for 3-5h, then add a composite enzyme for enzymolysis, inactivate the enzyme in a boiling water bath to obtain an enzymolysis solution, add a 10% trichloroacetic acid solution of equal volume to the enzymolysis solution, centrifuge at 5000-6000rpm for 10-15min, take the supernatant, add 3 times the volume of anhydrous ethanol, let stand at 4°C for 12-24h, centrifuge at 2000-3000rpm for 8-10min, filter the precipitate, wash it with distilled water 3 times, and freeze-dry it to obtain Centella asiatica polysaccharide;

[0009] S2, adding 2-5% mass ratio of Centella asiatica polysaccharide to isopropanol, vigorously stirring for 30-40 min to form a suspension, then adding 2 times volume of 3 mol / L sodium hydroxide solution, stirring at 15-25 ° C for 1-2 h, adding chloroacetic acid to adjust the pH to neutral, reacting at 50-60 ° C for 3-5 h, cooling to room temperature, adjusting the pH to 7.0, dialyzing, alcohol precipitation, freeze drying, and obtaining carboxymethylated Centella asiatica polysaccharide;

[0010] S3, after the dried mung bean is crushed, it is mixed with 3 times the mass of phosphate buffer, homogenized 3 times at 10000r / min, each time for 30s, passed through a 20-mesh filter, centrifuged at 2000r / min for 15min, the precipitate is collected, and the above homogenization and centrifugation steps are repeated twice to obtain mung bean protein precipitate, the mung bean protein precipitate is dispersed in 4 times the volume of 0.1mol / L NaCl solution, low speed homogenization for 30s, centrifuged at 2000r / min for 15min, repeated twice to obtain a purified mung bean protein solution, the mung bean protein solution is mixed with a 2% chitosan solution in a volume ratio of 1:1, the pH value is adjusted to 6.0, and magnetic stirring is performed at room temperature for 4h to obtain a mung bean protein-chitosan complex;

[0011] S4. Carboxymethylated Centella asiatica polysaccharide and mung bean protein-chitosan complex are evenly mixed in a mass ratio of 2:1, added into distilled water, stirred in a water bath at 70-80°C at a speed of 1000-2000 rpm for 10-12 min, freeze-dried, and the freeze-dried powder is placed in a constant temperature drying oven at 70°C for 24-48 h to obtain an antipruritic complex.

[0012] Furthermore, the complex enzyme comprises the following components in parts by weight: 2-3 parts of cellulase, 2-3 parts of pectinase and 1-2 parts of glucosidase, and the added amount of the complex enzyme is 0.5%-1.5% of the mass of the Centella asiatica extract.

[0013] Furthermore, the phosphate buffer comprises the following components: 10 mmol / L Na2HPO4 / NaH2PO4, 0.1 mmol / L NaCl, 2 mmol / L MgCl2, and 1 mmol / L EGTA.

[0014] Furthermore, the polypeptide is a combination of one or more of hexapeptide-8, hexapeptide-9, hexapeptide-10, hexanoyl tetrapeptide-3, heptapeptide, palmitoyl dipeptide-5, collagen peptide, elastin peptide, bird's nest peptide, and glutathione.

[0015] Furthermore, the thickener is one of cetyl alcohol, xanthan gum, carrageenan, sodium alginate and carbomer.

[0016] Furthermore, the moisturizing agent is one of glyceryl monostearate, sorbitan laurate, polyethylene glycol stearate and lecithin.

[0017] Furthermore, the moisturizing agent is one of propylene glycol, glycerin, pentylene glycol, xylitol and sorbitol.

[0018] On the other hand, the present invention also provides a method for preparing a hot spring water composition for relieving itching and soothing the skin, comprising the following steps:

[0019] (1) adding water to a reactor that has been strictly cleaned and dried, setting the speed to 300-500 rpm, and slowly adding water, moisturizer, thickener, and emulsifier to the reactor in sequence. After the addition is completed, slowly heating to 70-80° C., stirring continuously during the heating process to fully dissolve and evenly mix the components, controlling the heating rate at 1-2° C. / min, and continuously stirring at 70-80° C. for 20-30 min to ensure that the components are completely dissolved and evenly mixed to form a uniform mixing system;

[0020] (2) After the mixture is completely dissolved and uniformly dissolved, the temperature is slowly lowered at a rate of 0.5-1°C / min to 40-50°C, and the polypeptide, pyrroloquinoline quinone disodium salt, PCA zinc, calcium pantothenate, bisabolol, paeonol, antipruritic complex, Zanthoxylum bungeanum fruit extract, Perilla leaf extract, grape seed extract, and hot spring water are slowly added to the reaction kettle in sequence. After the addition of each component is completed, stirring is continued at 40-50°C for 30-40 minutes to fully mix the components;

[0021] (3) Reduce the stirring speed to 100-200 rpm, continue stirring at room temperature for 30-40 min, then transfer to a sterilized storage container and seal for storage.

[0022] The beneficial effects of the present invention are as follows: after the good film-forming property of chitosan is used to form a complex with mung bean protein, etc., after being applied to the skin surface, it can help build a relatively stable protective film with certain air permeability, which can reduce the occurrence of skin allergies and inflammatory reactions, form a relatively moist microenvironment on the skin surface, reduce the loss of skin moisture, and help maintain the moisture balance of the skin, thereby relieving uncomfortable symptoms such as dry skin and tightness, and further promoting skin relief; after the Centella asiatica polysaccharide in the present invention is modified by carboxymethylation, its water solubility and biological activity are significantly improved, and the carboxymethylated Centella asiatica polysaccharide and the mung bean protein-chitosan complex work synergistically, which can not only enhance the barrier function of the skin, but also promote the repair and regeneration of damaged skin cells by regulating the metabolism of skin cells; the perilla leaf extract and the grape seed extract are rich in various antioxidant substances, such as polyphenol compounds, etc., which can effectively remove free radicals in the skin, delay the aging process of the skin, enhance the antioxidant capacity of the skin, and keep the skin young and healthy. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The evaluation results of the antipruritic effect of the composition of the present invention;

[0024] Figure 2 The results are the evaluation results of the skin soothing effect of the composition of the present invention.

[0025] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0027] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein may be applied to the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only and are not intended to limit the content of this application.

[0028] The experimental methods in the following examples are conventional methods unless otherwise specified, and the experimental materials used in the following examples are purchased from commercial channels unless otherwise specified.

[0029] Example 1

[0030] A hot spring water composition for relieving itching and soothing the skin, comprising the following components in parts by weight:

[0031] 1 part of polypeptide (hexapeptide-8), 0.1 part of thickener (cetyl alcohol), 0.2 part of pyrroloquinoline quinone disodium salt, 0. part of PCA zinc, 0.1 part of calcium pantothenate, 0.3 part of bisabolol, 0.2 part of paeonol, 0.5 part of antipruritic complex, 0.5 part of Zanthoxylum bungeanum fruit extract, 0.1 part of perilla leaf extract, 0.1 part of grape seed extract, 15 parts of hot spring water, 0.5 part of emulsifier, 5 parts of moisturizer (propylene glycol), and 50 parts of water.

[0032] The antipruritic compound is prepared by the following steps:

[0033] S1. Add 1% of the mass ratio of Centella asiatica extract to deionized water, treat in a constant temperature water bath at 40°C for 3h, then add complex enzyme (2 parts of cellulase, 2 parts of pectinase and 1 part of glucosidase, the addition amount is 0.5% of the mass of Centella asiatica extract) for enzymolysis, inactivate the enzyme in a boiling water bath to obtain an enzymolysis solution, add a 10% mass fraction of trichloroacetic acid solution equal to the volume of the enzymolysis solution, centrifuge at 5000rpm for 10min, take the supernatant, add 3 times the volume of anhydrous ethanol, let stand at 4°C for 12h, centrifuge at 2000rpm for 8min, filter the precipitate, wash it with distilled water 3 times, and freeze-dry it to obtain Centella asiatica polysaccharide;

[0034] S2, adding Centella asiatica polysaccharide to isopropanol at a mass ratio of 2%, vigorously stirring for 30 minutes to form a suspension, then adding 2 volumes of 3 mol / L sodium hydroxide solution, stirring at 15°C for 1 hour, adding chloroacetic acid to adjust the pH to neutral, reacting at 50°C for 3 hours, cooling to room temperature, adjusting the pH to 7.0, dialyzing, alcohol precipitation, and freeze-drying to obtain carboxymethylated Centella asiatica polysaccharide;

[0035] S3, after the dried mung bean is crushed, it is mixed with 3 times the mass of phosphate buffer (10mmol / L Na2HPO4 / NaH2PO4, 0.1mmol / L NaCl, 2mmol / L MgCl2, 1mmol / L EGTA), homogenized at 10000r / min for 3 times, each time for 30s, passed through a 20-mesh filter, centrifuged at 2000r / min for 15min, the precipitate is collected, and the above homogenization and centrifugation steps are repeated twice to obtain mung bean protein precipitate, the mung bean protein precipitate is dispersed in 4 times the volume of 0.1mol / L NaCl solution, homogenized at low speed for 30s, centrifuged at 2000r / min for 15min, and repeated twice to obtain a purified mung bean protein solution, the mung bean protein solution is mixed with a 2% chitosan solution in a volume ratio of 1:1, the pH value is adjusted to 6.0, and magnetic stirring is performed at room temperature for 4h to obtain a mung bean protein-chitosan complex;

[0036] S4. Carboxymethylated Centella asiatica polysaccharide and mung bean protein-chitosan complex were evenly mixed in a mass ratio of 2:1, added into distilled water, stirred in a 70°C water bath at 1000 rpm for 10 min, freeze-dried, and the freeze-dried powder was placed in a constant temperature drying oven at 70°C for 24 h to obtain an antipruritic complex.

[0037] A method for preparing a hot spring water composition for relieving itching and soothing the skin comprises the following steps:

[0038] (1) Add water to a reactor that has been strictly cleaned and dried, set the speed to 300 rpm, and slowly add water, moisturizer, thickener, and emulsifier to the reactor in sequence. After the addition is completed, slowly heat it to 70° C., and continue stirring during the heating process to fully dissolve and evenly mix the components. The heating rate is controlled at 1° C. / min, and stirring is continued at 70° C. for 20 min to ensure that the components are completely dissolved and evenly mixed to form a uniform mixing system;

[0039] (2) After the mixture is completely dissolved and uniformly dissolved, the temperature is slowly lowered at a rate of 0.5°C / min to 40°C, and the polypeptide, pyrroloquinoline quinone disodium salt, PCA zinc, calcium pantothenate, bisabolol, paeonol, antipruritic complex, Zanthoxylum bungeanum fruit extract, Perilla leaf extract, grape seed extract, and hot spring water are slowly added to the reactor in sequence. After the addition of each component is completed, stirring is continued at 40°C for 30 minutes to ensure that the components are fully mixed;

[0040] (3) The stirring speed was reduced to 100 rpm, and stirring was continued at room temperature for 30 min. The mixture was then transferred to a sterilized storage container and sealed for storage.

[0041] Example 2

[0042] A hot spring water composition for relieving itching and soothing the skin, comprising the following components in parts by weight:

[0043] 4 parts of polypeptides (collagen peptide, elastin peptide, bird's nest peptide, and glutathione are mixed in a mass ratio of 2:1:1:1), 0.25 parts of thickener (carbomer), 0.6 parts of pyrroloquinoline quinone disodium salt, 0.5 parts of PCA zinc, 0.5 parts of calcium pantothenate, 0.5 parts of bisabolol, 0.5 parts of paeonol, 1.5 parts of antipruritic complex, 2 parts of Zanthoxylum bungeanum fruit extract, 3 parts of perilla leaf extract, 3 parts of grape seed extract, 20 parts of hot spring water, 2 parts of emulsifier, 15 parts of moisturizer (sorbitol), and 80 parts of water.

[0044] The antipruritic compound is prepared by the following steps:

[0045] S1. Add 2% of the mass ratio of Centella asiatica extract to deionized water, treat in a constant temperature water bath at 50°C for 5h, then add complex enzyme (3 parts of cellulase, 3 parts of pectinase and 2 parts of glucosidase, the addition amount is 1.5% of the mass of Centella asiatica extract) for enzymolysis, inactivate the enzyme in a boiling water bath to obtain an enzymolysis solution, add a 10% mass fraction of trichloroacetic acid solution equal to the volume of the enzymolysis solution, centrifuge at 6000rpm for 15min, take the supernatant, add 3 times the volume of anhydrous ethanol, let stand at 4°C for 24h, centrifuge at 3000rpm for 10min, filter the precipitate, wash it with distilled water 3 times, and freeze-dry it to obtain Centella asiatica polysaccharide;

[0046] S2, adding 5% mass ratio of Centella asiatica polysaccharide to isopropanol, vigorously stirring for 40 minutes to form a suspension, then adding 2 times volume of 3 mol / L sodium hydroxide solution, stirring at 25°C for 2 hours, adding chloroacetic acid to adjust the pH to neutral, reacting at 60°C for 5 hours, cooling to room temperature, adjusting the pH to 7.0, dialyzing, alcohol precipitation, freeze drying, and obtaining carboxymethylated Centella asiatica polysaccharide;

[0047] S3, after the dried mung bean is crushed, it is mixed with 3 times the mass of phosphate buffer (10mmol / L Na2HPO4 / NaH2PO4, 0.1mmol / L NaCl, 2mmol / L MgCl2, 1mmol / L EGTA), homogenized at 10000r / min for 3 times, each time for 30s, passed through a 20-mesh filter, centrifuged at 2000r / min for 15min, the precipitate is collected, and the above homogenization and centrifugation steps are repeated twice to obtain mung bean protein precipitate, the mung bean protein precipitate is dispersed in 4 times the volume of 0.1mol / L NaCl solution, homogenized at low speed for 30s, centrifuged at 2000r / min for 15min, and repeated twice to obtain a purified mung bean protein solution, the mung bean protein solution is mixed with a 2% chitosan solution in a volume ratio of 1:1, the pH value is adjusted to 6.0, and magnetic stirring is performed at room temperature for 4h to obtain a mung bean protein-chitosan complex;

[0048] S4. Carboxymethylated Centella asiatica polysaccharide and mung bean protein-chitosan complex were evenly mixed in a mass ratio of 2:1, added into distilled water, stirred in a water bath at 80°C at 2000 rpm for 12 min, freeze-dried, and the freeze-dried powder was placed in a constant temperature drying oven at 70°C for 48 h to obtain an antipruritic complex.

[0049] A method for preparing a hot spring water composition for relieving itching and soothing the skin comprises the following steps:

[0050] (1) Add water to a reactor that has been strictly cleaned and dried, set the speed to 500 rpm, and slowly add water, moisturizer, thickener, and emulsifier to the reactor in sequence. After the addition is completed, slowly heat it to 80° C., and continue stirring during the heating process to fully dissolve and evenly mix the components. The heating rate is controlled at 2° C. / min, and stirring is continued at 80° C. for 30 min to ensure that the components are completely dissolved and evenly mixed to form a uniform mixing system;

[0051] (2) After the mixture is completely dissolved and uniformly dissolved, the temperature is slowly lowered at a rate of 1°C / min to 50°C, and the polypeptide, pyrroloquinoline quinone disodium salt, PCA zinc, calcium pantothenate, bisabolol, paeonol, antipruritic complex, Zanthoxylum bungeanum fruit extract, Perilla leaf extract, grape seed extract, and hot spring water are slowly added to the reactor in sequence. After the addition of each component is completed, stirring is continued at 50°C for 40 minutes to ensure that the components are fully mixed;

[0052] (3) The stirring speed was reduced to 200 rpm, and stirring was continued at room temperature for 40 min. The mixture was then transferred to a sterilized storage container and sealed for storage.

[0053] Example 3

[0054] A hot spring water composition for relieving itching and soothing the skin, comprising the following components in parts by weight:

[0055] 2.5 parts of polypeptides (hexapeptide-9, hexapeptide-10, hexanoyl tetrapeptide-3, heptapeptide, and palmitoyl dipeptide-5 are mixed in a mass ratio of 2:1:2:1:1), 0.18 parts of thickener (xanthan gum), 0.4 parts of pyrroloquinoline quinone disodium salt, 0.2 parts of PCA zinc, 0.2 parts of calcium pantothenate, 0.4 parts of bisabolol, 0.3 parts of paeonol, 1 part of antipruritic complex, 1.2 parts of Zanthoxylum bungeanum fruit extract, 1.5 parts of perilla leaf extract, 1.5 parts of grape seed extract, 18 parts of hot spring water, 1.25 parts of emulsifier, 10 parts of humectant (glycerin), and 65 parts of water.

[0056] The antipruritic compound is prepared by the following steps:

[0057] S1. Add the Centella asiatica extract to deionized water at a mass ratio of 1.5%, treat in a constant temperature water bath at 45°C for 4h, then add a composite enzyme (2.5 parts of cellulase, 2.5 parts of pectinase and 1.5 parts of glucosidase, the added amount is 1% of the mass of the Centella asiatica extract) for enzymolysis, inactivate the enzyme in a boiling water bath to obtain an enzymolysis solution, add a 10% trichloroacetic acid solution of the same volume as the enzymolysis solution, centrifuge at 5500rpm for 12.5min, take the supernatant, add 3 times the volume of anhydrous ethanol, let stand at 4°C for 18h, centrifuge at 2500rpm for 9min, filter the precipitate, wash it 3 times with distilled water, and freeze-dry it to obtain Centella asiatica polysaccharide;

[0058] S2, add Centella asiatica polysaccharide to isopropanol at a mass ratio of 3.5%, stir vigorously for 35 minutes to form a suspension, then add 2 volumes of 3 mol / L sodium hydroxide solution, stir at 20°C for 1.5 hours, add chloroacetic acid to adjust the pH to neutral, react at 55°C for 4 hours, cool to room temperature, adjust the pH to 7.0, dialyze, precipitate with alcohol, and freeze-dry to obtain carboxymethylated Centella asiatica polysaccharide;

[0059] S3, after the dried mung bean is crushed, it is mixed with 3 times the mass of phosphate buffer (10mmol / L Na2HPO4 / NaH2PO4, 0.1mmol / L NaCl, 2mmol / L MgCl2, 1mmol / L EGTA), homogenized at 10000r / min for 3 times, each time for 30s, passed through a 20-mesh filter, centrifuged at 2000r / min for 15min, the precipitate is collected, and the above homogenization and centrifugation steps are repeated twice to obtain mung bean protein precipitate, the mung bean protein precipitate is dispersed in 4 times the volume of 0.1mol / L NaCl solution, homogenized at low speed for 30s, centrifuged at 2000r / min for 15min, and repeated twice to obtain a purified mung bean protein solution, the mung bean protein solution is mixed with a 2% chitosan solution in a volume ratio of 1:1, the pH value is adjusted to 6.0, and magnetic stirring is performed at room temperature for 4h to obtain a mung bean protein-chitosan complex;

[0060] S4. Carboxymethylated Centella asiatica polysaccharide and mung bean protein-chitosan complex were mixed evenly in a mass ratio of 2:1, added into distilled water, stirred in a 75°C water bath at 1500 rpm for 11 min, freeze-dried, and the freeze-dried powder was placed in a constant temperature drying oven at 70°C for 36 h to obtain an antipruritic complex.

[0061] A method for preparing a hot spring water composition for relieving itching and soothing the skin comprises the following steps:

[0062] (1) Add water to a reactor that has been strictly cleaned and dried, set the speed to 400 rpm, and slowly add water, moisturizer, thickener, and emulsifier to the reactor in sequence. After the addition is completed, slowly heat it to 75° C., and continue stirring during the heating process to fully dissolve and evenly mix the components. The heating rate is controlled at 1.5° C. / min, and stirring is continued at 75° C. for 25 minutes to ensure that the components are completely dissolved and evenly mixed to form a uniform mixing system;

[0063] (2) After the mixture is completely dissolved and uniformly dissolved, the temperature is slowly lowered at a rate of 0.75°C / min to 45°C, and the polypeptide, pyrroloquinoline quinone disodium salt, PCA zinc, calcium pantothenate, bisabolol, paeonol, antipruritic complex, Zanthoxylum bungeanum fruit extract, Perilla leaf extract, grape seed extract, and hot spring water are slowly added to the reactor in sequence. After the addition of each component is completed, stirring is continued at 45°C for 35 minutes to ensure that the components are fully mixed;

[0064] (3) The stirring speed was reduced to 150 rpm, and the stirring was continued at room temperature for 35 min. The mixture was then transferred to a sterilized storage container and sealed for storage.

[0065] Comparative Example 1

[0066] The difference between this comparative example and Example 3 is that an equal amount of carboxymethylated Centella asiatica polysaccharide is used to replace the antipruritic complex, and the rest is the same as Example 3.

[0067] Comparative Example 2

[0068] The difference between this comparative example and Example 3 is that an equal amount of chitosan is used to replace the mung bean protein-chitosan complex, and the rest is the same as Example 3.

[0069] Results Analysis

[0070] Test Example 1

[0071] Safety evaluation of the composition

[0072] Male C57BL / 6 mice aged 6-8 weeks were selected and divided into 7 groups, namely a positive control group, a blank group and an antipruritic composition group. The back of the neck of the mice was shaved before the experiment, and then the positive control group was smeared with the positive control drug dexamethasone ointment, and the antipruritic composition group was smeared with the composition of each embodiment and comparative example, and washed with warm water after 30 minutes, and observed whether erythema and edema appeared in the neck of the mice 1 hour and 24 hours after washing. The experimental results are shown in Table 1.

[0073] From the results in Table 1, it can be seen that when Examples 1-3 and Comparative Examples 1 and 2 were applied to the back of the neck of mice, no erythema or edema appeared on the neck of the mice 1 hour and 24 hours after washing. The positive control group developed erythema (without edema) after 24 hours, and the blank group had no erythema or edema throughout. This shows that the hot spring water composition for relieving itching and soothing the skin involved in the embodiments of the present invention, as well as the composition of the relevant comparative examples, are less irritating to the skin in a short time and a relatively long time, and have higher safety. The erythema in the positive control group may be due to the fact that its control drug dexamethasone ointment has certain skin irritation, while the composition of the present invention, compared with it, shows good safety in use, which provides an important premise for subsequent application in skin care.

[0074] Table 1 Composition safety evaluation results

[0075] Serial number 1h 24h Example 1 No erythema or edema No erythema or edema Example 1 No erythema or edema No erythema or edema Example 1 No erythema or edema No erythema or edema Comparative Example 1 No erythema or edema No erythema or edema Comparative Example No erythema or edema No erythema or edema Positive control group No erythema or edema There is erythema but no edema Blank Group No erythema or edema No erythema or edema

[0076] Test Example 2

[0077] Evaluation of the antipruritic effect of the composition

[0078] (1) Mouse preparation stage: 6-8 week old male C57BL / 6 mice were selected and bred in an SPF animal laboratory for 3-5 days after purchase. 48 hours before the start of the experiment, the hair on the back of the neck of the mice was carefully removed with a razor blade. The mice were then placed in a 4.5×4.5×7 inch box for 30 minutes. This operation was performed at the same time every day for two consecutive days to allow the mice to become familiar with the environment.

[0079] (2) Grouping and drug administration: The mice were randomly divided into a blank control group, a positive control group, and an antipruritic composition group, with 8 mice in each group. Two hours before the start of the experiment, the mice were treated at the shaved area on the back of their necks. The blank control group was not treated. The positive control group was treated with a positive control drug, dexamethasone ointment. The antipruritic composition group was treated with the composition of each embodiment and comparative example, with an application area of ​​1 cm×1 cm and a thickness of 1-2 mm. The operation was gentle and standardized to ensure that the drug was evenly distributed and did not spill over.

[0080] (3) Induction of itch model and behavioral observation: Two hours after drug administration, the itch model was induced in mice. Histamine (100 μM, 50 μL) was injected subcutaneously into the shaved area on the back of the neck of the mice using an insulin injection needle. During the operation, the mice were fixed to keep the skin on the back of the neck taut. The needle was inserted into the subcutaneous tissue at an angle of about 30° to the skin. The drug was slowly injected to ensure that there was no leakage. The mice were immediately placed in an observation box and video recording was started. The video recording lasted for 45 minutes. During the video recording, the behavior of the mice was observed. The behavior of the mice lifting their hind legs to scratch the back of the neck was recorded as an effective scratching behavior. The average number of scratching times of each group of mice within 45 minutes was recorded.

[0081] The above test results can be found in Figure 1 .

[0082] Depend on Figure 1 It can be seen that compared with the blank control group, Examples 1-3 significantly inhibited the histamine-induced itch response of mice, and the number of scratching times was greatly reduced. From the neurophysiological mechanism of itch, this is due to the synergistic effect of multiple components in the composition, among which the polypeptide may intervene in the itch signal transduction by regulating the release of neuropeptides or the interaction with skin nerve endings; the carboxymethylated Centella asiatica polysaccharide and the mung bean protein-chitosan complex in the antipruritic complex may synergistically play a role in regulating the skin immune microenvironment, repairing damaged skin tissue, etc., reducing the skin's sensitivity to external stimuli; ingredients such as Perilla leaf extract may also be involved in the regulation of itching-related inflammatory mediators, and after changing the key components of the antipruritic complex in Comparative Examples 1 and 2, the number of scratching times increased significantly, further confirming the unique formula of the antipruritic complex of the present invention and the indispensability and synergistic enhancement of the combination of the components of the entire composition in terms of antipruritic efficacy.

[0083] Test Example 3

[0084] Evaluation of the skin soothing effect of the composition

[0085] (1) Cell culture and treatment: cells expressing TRPV1 were seeded in 96-well plates or 35 mm culture dishes and cultured in a 37°C, 5% CO2 incubator until the logarithmic growth phase, so that the cell density reached 5000-10000 cells / well;

[0086] The experiment was divided into a control group, an agonist group (only the TRPV1 agonist capsaicin was added), an example group, and a comparative example group;

[0087] (2) TRPV1 activation and detection: Gently wash cells 2-3 times with serum-free medium to remove residual serum and impurities;

[0088] In a 96-well plate, add 100 μL of buffer containing 2 μM Fluo-4AM to each well and incubate at 37°C for 30-60 minutes to allow Fluo-4AM to enter the cells and be hydrolyzed by esterase into Fluo-4, which then generates fluorescence after binding to intracellular calcium ions.

[0089] After the incubation, the cells were washed again 2-3 times with serum-free medium to remove Fluo-4AM that did not enter the cells;

[0090] Add capsaicin solution with a final concentration of 1 μM to all groups, quickly place the 96-well plate into the microplate reader, monitor the changes in fluorescence intensity at an excitation wavelength of 488 nm and an emission wavelength of 525 nm, set the microplate reader to read the fluorescence value every 10 seconds for a total of 5-10 minutes, and record the calcium ion fluorescence peak value reached by each group during this period; the lower the calcium ion fluorescence peak value, the lower the degree of TRPV1 activation, and the stronger the inhibitory effect of cosmetics on TRPV1.

[0091] The above test results can be found in Figure 2 .

[0092] Depend on Figure 2 It can be seen that the calcium ion fluorescence peak corresponding to Examples 1-3 is significantly lower than that of the control group and the agonist group. From the perspective of skin sensory neurobiology, this shows that the composition of the present invention can effectively inhibit the activation of TRPV1 receptors, thereby reducing the skin's perception and response to external stimuli. Its mechanism of action may involve the synergistic effect of multiple components in the composition. The antioxidants in the perilla leaf extract and the grape seed extract can scavenge free radicals and reduce the oxidative damage and activation of free radicals to TRPV1 receptors and related cell signaling pathways; the Centella asiatica polysaccharide-related complex enhances the skin barrier function and reduces the chance of contact between external irritants and TRPV1 receptors, thereby synergistically achieving a soothing effect on the skin from multiple targets and multiple pathways. After the key ingredients are changed in Comparative Examples 1 and 2, the calcium ion fluorescence peak increases and the soothing effect weakens, which once again emphasizes the key significance and irreplaceability of the synergistic cooperation between the components of the composition of the present invention in soothing the skin, and provides a strong experimental basis for its application in the development of skin care products related to soothing the skin.

[0093] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0094] The present invention and its implementation methods are described above, which is not restrictive. The drawings are only one of the implementation methods of the present invention, and the actual application is not limited thereto. In short, if ordinary technicians in the field are inspired by it and design methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention, they should all fall within the protection scope of the present invention.

Claims

1. A hot spring water composition for relieving itching and soothing the skin, characterized in that: The invention comprises the following components in parts by weight: 1-4 parts of polypeptide, 0.1-0.25 parts of thickener, 0.2-0.6 parts of pyrroloquinoline quinone disodium salt, 0.1-0.5 parts of PCA zinc, 0.1-0.5 parts of calcium pantothenate, 0.3-0.5 parts of bisabolol, 0.2-0.5 parts of paeonol, 0.5-1.5 parts of antipruritic complex, 0.5-2 parts of Zanthoxylum bungeanum fruit extract, 0.1-3 parts of Perilla leaf extract, 0.1-3 parts of grape seed extract, 15-20 parts of hot spring water, 0.5-2 parts of emulsifier, 5-15 parts of moisturizer and 50-80 parts of water; The antipruritic compound is prepared by the following steps: S1. Add the Centella asiatica extract to deionized water at a mass ratio of 1-2%, treat in a constant temperature water bath at 40-50°C for 3-5h, then add a composite enzyme for enzymolysis, inactivate the enzyme in a boiling water bath to obtain an enzymolysis solution, add a 10% trichloroacetic acid solution of equal volume to the enzymolysis solution, centrifuge at 5000-6000rpm for 10-15min, take the supernatant, add 3 times the volume of anhydrous ethanol, let stand at 4°C for 12-24h, centrifuge at 2000-3000rpm for 8-10min, filter the precipitate, wash it with distilled water 3 times, and freeze-dry it to obtain Centella asiatica polysaccharide; S2, adding 2-5% mass ratio of Centella asiatica polysaccharide to isopropanol, vigorously stirring for 30-40 min to form a suspension, then adding 2 times volume of 3 mol / L sodium hydroxide solution, stirring at 15-25 ° C for 1-2 h, adding chloroacetic acid to adjust the pH to neutral, reacting at 50-60 ° C for 3-5 h, cooling to room temperature, adjusting the pH to 7.0, dialyzing, alcohol precipitation, freeze drying, and obtaining carboxymethylated Centella asiatica polysaccharide; S3, after the dried mung bean is crushed, it is mixed with 3 times the mass of phosphate buffer, homogenized 3 times at 10000r / min, each time for 30s, passed through a 20-mesh filter, centrifuged at 2000r / min for 15min, the precipitate is collected, and the above homogenization and centrifugation steps are repeated twice to obtain mung bean protein precipitate, the mung bean protein precipitate is dispersed in 4 times the volume of 0.1mol / L NaCl solution, low speed homogenization for 30s, centrifuged at 2000r / min for 15min, repeated twice to obtain a purified mung bean protein solution, the mung bean protein solution is mixed with a 2% chitosan solution in a volume ratio of 1:1, the pH value is adjusted to 6.0, and magnetic stirring is performed at room temperature for 4h to obtain a mung bean protein-chitosan complex; S4. Carboxymethylated Centella asiatica polysaccharide and mung bean protein-chitosan complex are evenly mixed in a mass ratio of 2:1, added into distilled water, stirred in a water bath at 70-80°C at a speed of 1000-2000 rpm for 10-12 min, freeze-dried, and the freeze-dried powder is placed in a constant temperature drying oven at 70°C for 24-48 h to obtain an antipruritic complex.

2. The hot spring water composition for relieving itching and soothing the skin according to claim 1, characterized in that: The complex enzyme comprises the following components in parts by weight: 2-3 parts of cellulase, 2-3 parts of pectinase and 1-2 parts of glucosidase. The added amount of the complex enzyme is 0.5%-1.5% of the mass of the Centella asiatica extract.

3. The hot spring water composition for relieving itching and soothing the skin according to claim 2, characterized in that: The phosphate buffer comprises the following components: 10 mmol / L Na2HPO4 / NaH2PO4, 0.1 mmol / L NaCl, 2 mmol / L MgCl2, and 1 mmol / L EGTA.

4. The hot spring water composition for relieving itching and soothing the skin according to claim 3, characterized in that: The polypeptide is a combination of one or more of hexapeptide-8, hexapeptide-9, hexapeptide-10, hexanoyl tetrapeptide-3, heptapeptide, palmitoyl dipeptide-5, collagen peptide, elastin peptide, bird's nest peptide, and glutathione.

5. The hot spring water composition for relieving itching and soothing the skin according to claim 4, characterized in that: The thickener is one of cetyl alcohol, xanthan gum, carrageenan, sodium alginate and carbomer.

6. The hot spring water composition for relieving itching and soothing the skin according to claim 5, characterized in that: The moisturizing agent is one of glyceryl monostearate, sorbitan laurate, polyethylene glycol stearate and lecithin.

7. The hot spring water composition for relieving itching and soothing the skin according to claim 6, characterized in that: The moisturizing agent is one of propylene glycol, glycerin, pentylene glycol, xylitol and sorbitol.

8. A method for preparing the hot spring water composition for relieving itching and soothing the skin according to any one of claims 1 to 7, characterized in that: The steps include: (1) adding water to a reactor that has been strictly cleaned and dried, setting the speed to 300-500 rpm, and slowly adding water, moisturizer, thickener, and emulsifier to the reactor in sequence. After the addition is completed, slowly heating to 70-80° C., stirring continuously during the heating process to fully dissolve and evenly mix the components, controlling the heating rate at 1-2° C. / min, and continuously stirring at 70-80° C. for 20-30 min to ensure that the components are completely dissolved and evenly mixed to form a uniform mixing system; (2) After the mixture is completely dissolved and uniformly dissolved, the temperature is slowly lowered at a rate of 0.5-1°C / min to 40-50°C, and the polypeptide, pyrroloquinoline quinone disodium salt, PCA zinc, calcium pantothenate, bisabolol, paeonol, antipruritic complex, Zanthoxylum bungeanum fruit extract, Perilla leaf extract, grape seed extract, and hot spring water are slowly added to the reaction kettle in sequence. After the addition of each component is completed, stirring is continued at 40-50°C for 30-40 minutes to fully mix the components; (3) Reduce the stirring speed to 100-200 rpm, continue stirring at room temperature for 30-40 min, then transfer to a sterilized storage container and seal for storage.

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