High-activity probiotic dropping pill and preparation method thereof

Abstract

The invention discloses a high-activity probiotic dropping pill and a preparation method thereof. The dropping pill comprises a core material and a wall material, and the wall material is prepared from the following raw materials in parts by weight: 5-15 parts of carboxymethyl cellulose, 1-5 parts of sodium alginate, 5-10 parts of gelatin and 80-100 parts of pure water; the core material is prepared from the following raw materials in parts by weight: 5-10 parts of ganoderma lucidum spore oil, 0.5-2 parts of probiotic powder, 1.5-2 parts of whey protein, 0.5-1 part of sodium alginate and 0.2-0.5 part of sucrose ester; 15 to 20 parts by weight of pure water; wherein the mass ratio of the wall material to the core material is 10: (2-4). The probiotics are uniformly dispersed in the core material by using the ganoderma lucidum spore oil, and the activity of the probiotics in the shelf life can be maintained by using the physiological activities of the ganoderma lucidum spore oil, such as oxidation resistance, bacteria resistance and inflammation resistance.

Description

Technical Field

[0001] The invention belongs to the field of preparation of probiotic products, and particularly relates to a high-activity probiotic dropping pill and a preparation method thereof. Background Art

[0002] Probiotics refer to active microorganisms that are beneficial to the host. When ingested in sufficient quantities, they can have a positive impact on the host's health. They are usually found in the human intestines, helping to maintain the balance of intestinal microbiota and promote the health of the digestive system. There are many types of probiotics, the most common of which are: Lactobacillus, Bifidobacterium, yeast, etc. A large number of studies have confirmed that probiotics have multiple benefits for human health, such as slowing the development of chronic diseases, regulating the immune system, improving nutritional status, and maintaining the balance of intestinal microecology. However, probiotics are often fragile and sensitive to adverse environmental factors such as oxygen and gastric acid, and exhibit problems such as poor acid resistance and difficulty in storage.

[0003] Relevant studies have shown that supplemented probiotics can only produce beneficial effects in the human intestine when they reach a certain number level, such as 1*106cfu / g. Therefore, the relevant probiotics need to ensure that the number of probiotics entering the human digestive system remains at a certain level during the shelf life, that is, to maintain the corresponding activity in order to play their probiotic effects. However, since the bacteria are living bacteria, they are very sensitive and fragile, and are easily affected by the surrounding environment such as temperature, oxygen, moisture, etc. and lose their activity. Therefore, it is necessary to develop a new type of highly active probiotic product to ensure that the probiotics can reach a certain number level when entering the human digestive system. Summary of the invention

[0004] The purpose of the present invention is to overcome the above-mentioned shortcomings and provide a high-activity probiotic dropping pill.

[0005] Another object of the present invention is to provide a method for preparing the above-mentioned highly active probiotic dropping pills.

[0006] The object of the present invention is achieved in the following ways:

[0007] A highly active probiotic dropping pill, comprising a core material and a wall material, wherein the core material is made of the following raw materials in parts by weight:

[0008] The wall material is made of the following raw materials in parts by weight:

[0009] 5-15 parts by weight of carboxymethyl cellulose, 1-5 parts by weight of sodium alginate, 5-10 parts by weight of gelatin, and 80-100 parts by weight of pure water;

[0010] The core material is made of the following raw materials in parts by weight:

[0011] 5-10 parts by weight of ganoderma lucidum spore oil, 0.5-2 parts by weight of probiotic powder, 1.5-2 parts by weight of whey protein, 0.5-1 part by weight of sodium alginate, 0.2-0.5 part by weight of sucrose ester; 15-20 parts by weight of pure water;

[0012] Among them, the mass ratio of wall material to core material is 10:2~4.

[0013] Preferably, the mass ratio of the wall material to the core material is 10:2-3.

[0014] Preferably, the wall material is made of the following raw materials in parts by weight: 6 parts by weight of carboxymethyl cellulose; 4-5 parts by weight of sodium alginate; 8-9 parts by weight of gelatin, and 80-82 parts by weight of pure water.

[0015] Preferably, the core material is prepared from the following raw materials in percentage by mass:

[0016] 8-10 parts by weight of ganoderma lucidum spore oil, 0.8-2 parts by weight of probiotic powder, 1.8-2 parts by weight of whey protein, 0.8-1 parts by weight of sodium alginate, 0.4 parts by weight of sucrose ester; and 15-20 parts by weight of pure water.

[0017] Preferably, the probiotic powder is Bacillus coagulans BC30.

[0018] The preparation method of the above-mentioned highly active probiotics comprises the following steps:

[0019] a) mixing carboxymethyl cellulose, sodium alginate, gelatin and pure water in proportion to obtain a wall material mixture;

[0020] b) mixing pure water, whey protein and sodium alginate in proportion, and then adding sucrose ester, ganoderma lucidum spore oil and probiotic powder to mix to obtain a core material mixed solution;

[0021] c) The core material mixed solution and the wall material mixed solution are mixed and prepared into probiotic dropping pills by using a dropping pill machine.

[0022] Preferably, the specific operation of step a) is: add pure water into the vacuum tank, start stirring, add carboxymethyl cellulose and sodium alginate, raise the temperature to 90°C while stirring, then add gelatin and mix well, stir for 1 hour, raise the temperature to 92°C, and pump into the wall material insulation tank of the pill machine.

[0023] Preferably, the specific operation of step b) is: adding pure water to a vacuum tank, starting stirring, adding whey protein and sodium alginate, heating to 92°C while stirring, then adding sucrose ester, continuing to stir at 90°C for 30 minutes, then cooling to 65°C, adding 1 / 2 to 2 / 3 of the total amount of Ganoderma lucidum spore oil, cooling to 50°C while stirring, and then adding the remaining Ganoderma lucidum spore oil pre-dispersed probiotic powder, stirring evenly and then pumping into the dripping pill machine core material insulation tank, maintaining the temperature at 45 to 50°C.

[0024] Preferably, the specific operation of step c) is: dropping pills in a pill machine, dropping into food-grade liquid paraffin with circulation cooling at 7°C at a core material speed of 0.3-0.5 ml / min and a wall material speed of 0.6-1.0 ml / min, to obtain probiotic pills.

[0025] Preferably, after obtaining the dropping pills in step c), the dropping pills are cooled and formed, placed in a 5% calcium chloride solution, soaked for 50 to 60 minutes, rinsed with pure water, and dried.

[0026] Preferably, step c) produces probiotic dropping pills with a particle size of 3 to 4 mm.

[0027] Compared with the prior art, the present invention has the following beneficial effects: the present invention uses ganoderma lucidum spore oil to evenly disperse the probiotics in the core material, and uses sucrose ester to form a water-in-oil solution to protect the activity of the probiotics, and the probiotics in the core material are evenly distributed in the ganoderma lucidum spore oil. Ganoderma lucidum spore oil can protect the cytoskeleton morphology of the probiotic bacteria and repair damaged cells, while the antioxidant, antibacterial, anti-inflammatory and other physiological activities of ganoderma lucidum spore oil can maintain the activity of the probiotics during the shelf life. DETAILED DESCRIPTION

[0028] The technical solution of the present invention is further described in detail below in conjunction with several preferred embodiments. This embodiment is implemented on the premise of the technical solution of the invention, and a detailed implementation method and specific operation process are given, but the protection scope of the present invention is not limited to the following embodiments.

[0029] The experimental methods described in the following examples are conventional methods unless otherwise specified, and are performed according to the techniques or conditions described in the literature in the field or according to the product instructions. The materials, reagents, etc. described in the following examples are all commercially available unless otherwise specified.

[0030] In the following examples, Ganoderma lucidum spore oil is a commercial product of Nanjing Zhongke Pharmaceutical Co., Ltd.; Bacillus coagulans BC30 (1.2×10 11 CFU / g), sucrose ester, whey protein, and carboxymethyl cellulose were purchased from Shandong Siyang Technology Co., Ltd.

[0031] Embodiment 1:

[0032] Add 80kg of pure water into the vacuum tank, start stirring, add 6kg of carboxymethyl cellulose and 5kg of sodium alginate, heat to 90℃ while stirring, add 9kg of gelatin and mix well, stir for 1h and heat to 92℃, pump into the wall material insulation tank of the pill machine, and keep warm at 50-60℃;

[0033] Add 16 kg of pure water to a vacuum tank, start stirring, add 1.8 kg of whey protein and 0.8 kg of sodium alginate, heat to 92 ° C while stirring, add 0.4 kg of sucrose ester, continue stirring at 90 ° C for 30 min, cool to 65 ° C, add 5 kg of Ganoderma lucidum spore oil, cool to 50 ° C while stirring, add 4 kg of Ganoderma lucidum spore oil, pre-disperse 2 kg of 1.2×10 11 CFU / g Bacillus coagulans BC30, stir evenly, pump into the pellet machine core material insulation tank, set the temperature to 45-50℃;

[0034] Adjust the pellet dropping speed to 0.3ml / min for the core material and 0.6ml / min for the wall material, and start dropping the pellets into food-grade liquid paraffin with circulation cooling at 7℃;

[0035] The pellets are cooled and formed, placed in a 5% calcium chloride solution tank and stirred and immersed, stirred for 60 seconds every 10-15 minutes, rinsed with pure water after 60 minutes and dried, and finally probiotic pellets with a particle size of 3-4 mm were obtained.

[0036] Embodiment 2:

[0037] Add 80kg of pure water into the vacuum tank, start stirring, add 6kg of carboxymethyl cellulose and 5kg of sodium alginate, heat to 90℃ while stirring, add 9kg of gelatin and mix well, stir for 1h and heat to 92℃, pump into the wall material insulation tank of the pill machine, and keep warm at 50-60℃;

[0038] In another vacuum tank, add 17 kg of pure water, start stirring, add 1.8 kg of whey protein and 1 kg of sodium alginate, heat to 92 ° C while stirring, add 0.4 kg of sucrose ester, continue stirring at 90 ° C for 30 min, cool to 65 ° C, add 6 kg of Ganoderma lucidum spore oil, cool to 50 ° C while stirring, add 3 kg of Ganoderma lucidum spore oil, pre-disperse 0.8 kg of 1.2×10 11 CFU / g Bacillus coagulans BC30, stir evenly, pump into the pellet machine core material insulation tank, set the temperature to 45-50℃;

[0039] Adjust the pellet core material speed to 0.5ml / min and the wall material speed to 1.0ml / min to start pellet dripping into food-grade liquid paraffin with circulation cooling at 7℃;

[0040] The pellets are cooled and formed, placed in a 5% calcium chloride solution tank and stirred and immersed, stirred for 60 seconds every 10-15 minutes, rinsed with pure water after 60 minutes and dried, and finally probiotic pellets with a particle size of 3-4 mm were obtained.

[0041] Embodiment 3:

[0042] Add 82kg of pure water into the vacuum tank, start stirring, add 6kg of carboxymethyl cellulose and 4kg of sodium alginate, heat to 90℃ while stirring, add 8kg of gelatin and mix well, stir for 1h and heat to 92℃, pump into the wall material insulation tank of the pill machine, and keep warm at 50-60℃;

[0043] Add 16 kg of pure water to a vacuum tank, start stirring, add 1.8 kg of whey protein and 0.8 kg of sodium alginate, heat to 92 ° C while stirring, add 0.4 kg of sucrose ester, continue stirring at 90 ° C for 30 min, cool to 65 ° C, add 5 kg of Ganoderma lucidum spore oil, cool to 50 ° C while stirring, add 4.5 kg of Ganoderma lucidum spore oil, pre-disperse 1.5 kg of 1.2 × 10 11 CFU / g Bacillus coagulans BC30, stir evenly, pump into the pellet machine core material insulation tank, set the temperature to 45-50℃;

[0044] Adjust the pellet dropping speed to 0.5ml / min for the core material and 0.9ml / min for the wall material, and start dropping the pellets into food-grade liquid paraffin with circulation cooling at 7℃;

[0045] The pellets are cooled and formed, placed in a 5% calcium chloride solution tank and stirred and immersed, stirred for 60 seconds every 10-15 minutes, rinsed with pure water after 60 minutes and dried, and finally probiotic pellets with a particle size of 3-4 mm were obtained.

[0046] Comparative Example 1

[0047] Add 80kg of pure water into the vacuum tank, start stirring, add 5kg of carboxymethyl cellulose and 7kg of sodium alginate, heat to 90℃ while stirring, add 8kg of gelatin and mix well, stir for 1h and heat to 92℃, pump into the wall material insulation tank of the pill machine and set aside;

[0048] Add 16 kg of pure water to the vacuum tank, start stirring, add 1.8 kg of whey protein and 0.8 kg of sodium alginate, heat to 92 °C while stirring, add 0.4 kg of sucrose ester, continue stirring at 90 °C for 30 min, cool to 65 °C, add 7 kg of olive oil, cool to 50 °C while stirring, add 2 kg of Ganoderma lucidum spore oil, pre-disperse 2 kg of 1.2 × 10 11 CFU / g Bacillus coagulans BC30, after stirring evenly, pump into the pellet machine core material insulation tank and maintain the temperature at 45-50℃;

[0049] Adjust the pellet dropping speed to 0.3ml / min for the core material and 0.6ml / min for the wall material, and start dropping the pellets into food-grade liquid paraffin with circulation cooling at 7℃;

[0050] The pellets are cooled and formed, placed in a 5% calcium chloride solution tank and stirred and immersed, stirred for 60 seconds every 10-15 minutes, rinsed with pure water after 60 minutes and dried, and finally the probiotic pellets were obtained.

[0051] Control group:

[0052] Add 80kg of pure water into the vacuum tank, start stirring, add 6kg of carboxymethyl cellulose and 5kg of sodium alginate, heat to 90℃ while stirring, add 9kg of gelatin and mix well, stir for 1h and heat to 92℃, pump into the wall material insulation tank of the pill machine and set aside;

[0053] Add 16 kg of pure water to a vacuum tank, start stirring, add 1.8 kg of whey protein and 0.8 kg of sodium alginate, heat to 92 °C while stirring, add 0.4 kg of sucrose ester, continue stirring at 90 °C for 30 min, then cool to 65 °C, add 5 kg of olive oil, stir for 10 min, cool to 50 °C while stirring, add 4 kg of olive oil, pre-disperse 2 kg of 1.2 × 10 11 CFU / g Bacillus coagulans BC30, after stirring evenly, pump into the pellet machine core material insulation tank and maintain the temperature at 45-50℃;

[0054] Adjust the pellet dropping speed to 0.3ml / min for the core material and 0.6ml / min for the wall material, and start dropping the pellets into food-grade liquid paraffin with circulation cooling at 7℃;

[0055] The pellets are cooled and formed, placed in a 5% calcium chloride solution tank and stirred and immersed, stirred for 60 seconds every 10-15 minutes, rinsed with pure water after 60 minutes and dried, and finally the probiotic pellets were obtained.

[0056] The probiotic pills were subjected to a long-term stability test at 18°C ​​± 2°C and RH 60% ± 5%. The number of viable bacteria was tested in October, January, March and June using the "GB 4789.35-2023 National Food Safety Standard Food Microbiology Test Lactic Acid Bacteria Test Colony Count Determination". The results are shown in the table below:

[0057]

[0058]

[0059] It can be seen from the above results that the comparative example encapsulating a small amount of Ganoderma lucidum spore oil is significantly better than the control group without encapsulating Ganoderma lucidum spore oil. The stability of the probiotic particles obtained by encapsulating Ganoderma lucidum spore oil in Examples 1-3 at room temperature is significantly higher than that of the comparative example, and the activity can still be maintained above 75% after storage for 6 months, which is significantly higher than that of the comparative example and the control group, and the activity of the probiotics can be improved.

[0060] The probiotic pills were subjected to an accelerated stability test at 30°C ± 2°C and RH 65% ± 5%. The number of viable bacteria was tested in October, January and February using the "GB 4789.35-2023 National Food Safety Standard Food Microbiology Test Lactic Acid Bacteria Test Colony Count Determination". The results are shown in the following table:

[0061]

[0062] It can be seen from the above results that under the conditions of the accelerated stability test, the number of live bacteria in the probiotic dripping pills of the embodiment is significantly higher than that of the embodiment and the comparative example, and the activity can still be maintained above 75% after storage for 2 months.

Claims

1. A highly active probiotic dropping pill, comprising a wall material and a core material, characterized in that The wall material is made of the following raw materials in parts by weight: 5-15 parts by weight of carboxymethyl cellulose, 1-5 parts by weight of sodium alginate, 5-10 parts by weight of gelatin, and 80-100 parts by weight of pure water; The core material is made of the following raw materials in parts by weight: 5-10 parts by weight of ganoderma lucidum spore oil, 0.5-2 parts by weight of probiotic powder, 1.5-2 parts by weight of whey protein, 0.5-1 part by weight of sodium alginate, 0.2-0.5 part by weight of sucrose ester; 15-20 parts by weight of pure water; Among them, the mass ratio of wall material to core material is 10:2~4.

2. The highly active probiotic dropping pill according to claim 1, characterized in that The wall material is made of the following raw materials in parts by weight: 6 parts by weight of carboxymethyl cellulose; 4-5 parts by weight of sodium alginate; 8-9 parts by weight of gelatin; 80-82 parts by weight of pure water.

3. The highly active probiotic dropping pill according to claim 1, characterized in that The core material is made of the following raw materials in parts by weight: 8-10 parts by weight of ganoderma lucidum spore oil, 0.8-2 parts by weight of probiotic powder, 1.8-2 parts by weight of whey protein, 0.8-1 part by weight of sodium alginate, and 0.4 part by weight of sucrose ester; 15 to 20 parts by weight of pure water.

4. The highly active probiotic dropping pill according to claim 1, characterized in that The probiotic powder is Bacillus coagulans BC30.

5. A method for preparing the highly active probiotics according to claim 1, characterized in that The method comprises the following steps: a) mixing carboxymethyl cellulose, sodium alginate, gelatin and pure water in proportion to obtain a wall material mixture; b) mixing pure water, whey protein and sodium alginate in proportion, and then adding sucrose ester, ganoderma lucidum spore oil and probiotic powder to mix to obtain a core material mixed solution; c) The core material mixed solution and the wall material mixed solution are mixed and prepared into probiotic dropping pills by using a dropping pill machine.

6. The method for preparing highly active probiotics according to claim 5, characterized in that The specific operation of step a) is as follows: add pure water into the vacuum tank, start stirring, add carboxymethyl cellulose and sodium alginate, raise the temperature to 90°C while stirring, then add gelatin and mix well, stir for 1 hour, raise the temperature to 92°C, and pump into the wall material insulation tank of the pill machine.

7. The method for preparing highly active probiotics according to claim 5, characterized in that The specific operation of step b) is as follows: adding pure water to a vacuum tank, starting stirring, adding whey protein and sodium alginate, heating to 92° C. while stirring, adding sucrose ester, continuing to stir at 90° C. for 30 minutes, cooling to 65° C., adding 1 / 2 to 2 / 3 of ganoderma lucidum spore oil, cooling to 50° C. while stirring, and then adding probiotic powder pre-dispersed with the remaining ganoderma lucidum spore oil, stirring evenly, and then pumping into a drip pill machine core material insulation tank, maintaining the temperature at 45 to 50° C.

8. The method for preparing highly active probiotics according to claim 5, characterized in that The specific operation of step c) is as follows: dropping pills in a pill machine, dropping into food-grade liquid paraffin with circulation cooling at 7° C. at a core material speed of 0.3-0.5 ml / min and a wall material speed of 0.6-1.0 ml / min, to obtain probiotic pills.

9. The method for preparing highly active probiotics according to claim 5, characterized in that Step c) After obtaining the dropping pills, the dropping pills are cooled and formed, placed in a 5% calcium chloride solution, soaked for 50 to 60 minutes, rinsed with pure water, and dried.

10. The method for preparing highly active probiotics according to claim 5, characterized in that Step c) obtaining probiotic dropping pills with a particle size of 3 to 4 mm.

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