Safflower seed oil compound preparation for improving skin inflammation of pet cats and dogs and preparation method of safflower seed oil compound preparation

Abstract

The invention provides a safflower seed oil compound preparation for improving skin inflammation of pet cats and dogs and a preparation method of the safflower seed oil compound preparation. The preparation method comprises the following steps: preparing safflower seed oil microcapsules; preparing deep sea fish oil microcapsules; preparing a chitosan solution as a cortex solution; taking safflower seed oil microcapsules, deep sea fish oil microcapsules and water, and uniformly stirring to obtain a core layer solution; carrying out wet spinning by taking ethanol as a coagulating bath to obtain chitosan fibers coated with safflower oil microcapsules and deep sea fish oil microcapsules; and drying and chopping to obtain the product. The fiber structure in which the safflower oil microcapsules and the deep sea fish oil microcapsules are loaded at the same time is adopted, the microcapsules are of a temperature response type, cats and dogs enter the oral cavity after ingestion, chewing is assisted, the microcapsules are broken, the safflower oil and the deep sea fish oil are released and mixed in the fiber, the oxidation speed cannot be affected under the anaerobic condition, and after entering the stomach, the safflower oil microcapsules and the deep sea fish oil microcapsules are released and mixed. Under gastric acid, outer layer fibers (pH response type) are dissolved, grease is dissolved out, and the effect starts to be achieved.

Description

Technical Field

[0001] The invention relates to the field of pet functional food, and in particular to a safflower seed oil compound preparation for improving skin inflammation of pet cats and dogs and a preparation method thereof. Background Art

[0002] Safflower is a herbaceous plant of the Asteraceae family. It is an economic crop that combines multiple uses such as oil, medicine, beverage, and natural pigment. Safflower seed oil is a yellow, clear liquid vegetable oil made from safflower seeds through extraction processes such as pressing. It is also called safflower oil. It is a special substance that combines medicinal materials, oils, and feeds. Safflower seed oil is a edible oil recognized by the world and has the effects of food, health care, and beauty. After analysis and identification, the main components of safflower seed oil are palmitic acid 6% to 8%, oleic acid 16% to 20%, stearic acid 2% to 3%, and linoleic acid 73% to 85%. In addition, safflower seed oil also contains natural VE, flavonoids and other active substances. Therefore, people give safflower seed oil the beautiful names of "King of Linoleic Acid" and "Crown of VE". Safflower seed oil has the effects of lowering blood lipids, lowering blood sugar, anti-oxidation, anti-aging, and anti-inflammatory. Wang Ren'ai studied the anti-inflammatory effect of safflower oil by mouse ear swelling experiment and toe swelling experiment. The results showed that the swelling rate of the drug-administered group was significantly reduced compared with the model group, and it had obvious anti-inflammatory effect. However, in actual application, due to the particularly high content of linoleic acid (ω-6 fatty acid) in safflower oil, when it enters the intestine, it will first become arachidonic acid, which attaches to the cell membrane and secretes metabolites to stimulate inflammation. There are also studies showing that consuming a large amount of linoleic acid can cause inflammation and increase the risk of ulcerative colitis. Therefore, how to balance the linoleic acid in safflower oil so that it can achieve an optimal anti-inflammatory effect is a problem that this patent urgently needs to solve. Summary of the invention

[0003] Technical problem to be solved: In view of the above technical problems, the purpose of the present invention is to provide a safflower seed oil compound preparation for improving skin inflammation of pet cats and dogs and a preparation method thereof, which adopts a fiber structure with safflower seed oil microcapsules and deep-sea fish oil microcapsules loaded inside. The microcapsules are temperature-responsive. After cats and dogs eat, they enter the mouth (temperature 38-39°C) and assist in chewing. The microcapsules rupture, and the safflower seed oil and deep-sea fish oil are released and mixed in the fibers. The oxidation rate is not affected under anaerobic conditions. After entering the stomach, the outer layer of fiber (pH-responsive) dissolves under gastric acid, and the oil dissolves and begins to exert its efficacy.

[0004] Technical solution: A safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs. The safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs is a fiber internally loaded with safflower seed oil microcapsules and deep-sea fish oil microcapsules; the material of the fiber is a pH-responsive material, and the membrane of the microcapsule is a temperature-responsive membrane. Furthermore, the pH response value of the pH responsive material is ≤5. Furthermore, the temperature response value of the temperature responsive membrane is ≥37.7°C. Furthermore, the deep-sea fish oil includes salmon oil, sardine oil, tuna oil and mackerel oil. The preparation method of the safflower seed oil compound preparation for improving skin inflammation of pet cats and dogs comprises the following steps: (1) Preparation of safflower oil microcapsules: Take modified gelatin, dissolve it in deionized water, add safflower oil, homogenize at a speed of 12000-15000 rpm to obtain a safflower oil crude emulsion, ultrasonicate for 2-3 min to obtain a safflower oil emulsion, freeze it in a -40°C refrigerator, freeze-dry it, and sieve it to obtain safflower oil microcapsules; (2) Preparation of deep-sea fish oil microcapsules: Take modified gelatin, dissolve it in deionized water, add deep-sea fish oil, homogenize at a speed of 13500-16000 rpm to obtain a deep-sea fish oil crude emulsion, ultrasonicate for 2-3 min to obtain a deep-sea fish oil emulsion, freeze it in a -40°C refrigerator, freeze-dry it, and sieve it to obtain deep-sea fish oil microcapsules; (3) taking chitosan, dissolving it in 1% acetic acid solution, and adding glycerol to obtain a chitosan solution with a mass fraction of 3-5%, which is used as the cortex solution; (4) taking safflower seed oil microcapsules, deep-sea fish oil microcapsules and water, and stirring them evenly to prepare a core layer solution; (5) using ethanol as a coagulation bath, wet spinning the cortex solution and the core solution, with the cortex spinning flow rate being 50 mL / h and the core spinning flow rate being 80 mL / h, to obtain chitosan fibers coated with safflower seed oil microcapsules and deep-sea fish oil microcapsules; (6) Drying and chopping into pieces of 1-2 mm to obtain a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs. Furthermore, the preparation method of the modified gelatin is as follows: Step 1: prepare a gelatin aqueous solution with a concentration of 20 mg / mL and adjust the pH to 9.00; Step 2: preparing a grape seed polyphenol aqueous solution with a concentration of 4 mg / mL and adjusting the pH to 9.00; Step 3: Mix the gelatin aqueous solution and the grape seed polyphenol aqueous solution in equal volumes, and stir at 36°C and 200-300 rpm for 20-24 hours; Step 4: Place in a dialysis bag with a molecular weight cutoff of 8000-14000Da and dialyze for 48 hours, replacing ultrapure water every 6 hours; Step 5: Freeze-dry to obtain modified gelatin. Furthermore, in the step (1), the mass ratio of modified gelatin, deionized water and safflower seed oil is 1:50:(0.5-0.8). Furthermore, in step (2), the mass ratio of modified gelatin, deionized water and deep-sea fish oil is 1:45:(0.4-0.7). Furthermore, in step (4), the ratio of safflower seed oil microcapsules to deep-sea fish oil microcapsules is 1:(3-5). Furthermore, the chitosan fibers coated with safflower seed oil microcapsules and deep-sea fish oil microcapsules can be twisted to form a twisted shape and then chopped. The application of the safflower seed oil compound preparation for improving skin inflammation of pet cats and dogs in anti-inflammatory products for pet cats and dogs. Beneficial effects: 1. Since the linoleic acid content in safflower seed oil is particularly high, up to 73%-85%, when the linoleic acid (ω-6 fatty acid) content is particularly high, it is easy to induce a super-physiological inflammatory response, causing chronic inflammation to exist for a long time, but having the effect of aggravating inflammation. Comparative Example 3 in the present invention also proves that its effect of being used alone is not good. Therefore, the present invention adopts safflower seed oil and deep-sea fish oil for compounding. Deep-sea fish oil is rich in ω-3 fatty acids. After the two are compounded, a better ω-6 / ω-3 ratio is achieved, thereby improving its anti-inflammatory effect. 2. The present invention adopts microencapsulation of safflower seed oil and deep-sea fish oil respectively, which solves the problem that direct mixing of safflower seed oil and deep-sea fish oil will accelerate the oxidation process, reduce the antioxidant stability and reduce the effect; on the other hand, cats and dogs are very sensitive to odors, and direct mixing of safflower seed oil and deep-sea fish oil produces a special odor, which causes them to reject eating. After microencapsulation, the odor can be covered up and does not affect their normal eating. 3. The present invention adopts a fiber structure with safflower seed oil microcapsules and deep-sea fish oil microcapsules loaded inside. The microcapsules are temperature-responsive. After cats and dogs eat, they enter the oral cavity (temperature 38-39°C) and assist in chewing. The microcapsules rupture, and the safflower seed oil and deep-sea fish oil are released and mixed in the fiber. The oxidation rate is not affected under anaerobic conditions. After entering the stomach, the outer fiber (pH-responsive) dissolves under gastric acid, and the oil dissolves and begins to exert its efficacy. 4. The present invention uses grape seed polyphenol to modify gelatin. On the one hand, it can improve the response temperature and pH stability of gelatin. It is stable at normal room temperature below 35°C, and can begin to disintegrate and dissolve fat at the oral temperature of cats and dogs (38-39°C); on the other hand, grape seed polyphenol can also improve the antioxidant property of gelatin and better protect fat. 5. The present invention uses chitosan as a raw material to prepare the fiber. Chitosan has good antibacterial properties, dissolves in gastric acid, and can effectively improve the storage stability of oils and fats. BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 This is a graph showing the change in peroxide value of oils and fats in Example 13 of the present invention and Comparative Example 4 over time. DETAILED DESCRIPTION The present invention proposes a safflower seed oil compound preparation for improving skin inflammation of pet cats and dogs and its application. In order to make the purpose, technical scheme and effect of the present invention clearer and more specific, the present invention will be further described in detail with examples below. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention. Example 1 The preparation method of modified gelatin is as follows: Step 1: prepare a gelatin aqueous solution with a concentration of 20 mg / mL and adjust the pH to 9.00; Step 2: preparing a grape seed polyphenol aqueous solution with a concentration of 4 mg / mL and adjusting the pH to 9.00; Step 3: Mix the gelatin aqueous solution and the grape seed polyphenol aqueous solution in equal volumes, and stir at 36°C and 250 rpm for 24 hours; Step 4: Place in a dialysis bag with a molecular weight cutoff of 8000-14000Da and dialyze for 48 hours, replacing ultrapure water every 6 hours; Step 5: Freeze-dry to obtain modified gelatin. In the measurement process of dynamic rheological properties, tan(δ)=1 is generally used as the turning point of melting, and the corresponding temperature is the melting temperature. In the dynamic viscoelastic analysis, the melting temperatures can be obtained respectively. The melting temperature of unmodified gelatin was determined to be 35.7°C, and the melting temperature of modified gelatin was 37.3°C, which increased by 1.6°C. Example 2 Preparation of safflower oil microcapsules: Take 1g of modified gelatin, dissolve it in 50g of deionized water, add 0.5g of safflower oil, homogenize at a speed of 14000rpm to obtain a crude safflower oil emulsion, ultrasonicate for 2min to obtain a safflower oil emulsion, put it in a -40℃ refrigerator to freeze, freeze-dry, and sieve to obtain safflower oil microcapsules. Example 3 Preparation of safflower oil microcapsules: Take 1g of modified gelatin, dissolve it in 50g of deionized water, add 0.7g of safflower oil, homogenize at a speed of 14000rpm to obtain a crude safflower oil emulsion, ultrasonicate for 2min to obtain a safflower oil emulsion, put it in a -40℃ refrigerator to freeze, freeze-dry, and sieve to obtain safflower oil microcapsules. Example 4 Preparation of safflower oil microcapsules: Take 1g of modified gelatin, dissolve it in 50g of deionized water, add 0.8g of safflower oil, homogenize at a speed of 14000rpm to obtain a crude safflower oil emulsion, ultrasonicate for 2min to obtain a safflower oil emulsion, put it in a -40℃ refrigerator to freeze, freeze-dry, and sieve to obtain safflower oil microcapsules. Example 5 Preparation of safflower oil microcapsules: Take 1g of modified gelatin, dissolve it in 50g of deionized water, add 0.7g of safflower oil, homogenize at a speed of 12000rpm to obtain a crude safflower oil emulsion, ultrasonicate for 2min to obtain a safflower oil emulsion, put it in a -40℃ refrigerator to freeze, freeze-dry, and sieve to obtain safflower oil microcapsules. Example 6 Preparation of safflower oil microcapsules: Take 1g of modified gelatin, dissolve it in 50g of deionized water, add 0.7g of safflower oil, homogenize at a speed of 15000rpm to obtain a crude safflower oil emulsion, ultrasonicate for 2min to obtain a safflower oil emulsion, put it in a -40℃ refrigerator to freeze, freeze-dry, and sieve to obtain safflower oil microcapsules. Comparative Example 1 The difference between this embodiment and embodiment 3 is that unmodified gelatin is used, specifically as follows: Preparation of safflower oil microcapsules: Take 1g of gelatin, dissolve it in 50g of deionized water, add 0.7g of safflower oil, homogenize at a speed of 14000rpm to obtain a crude safflower oil emulsion, ultrasonicate for 2min to obtain a safflower oil emulsion, put it in a -40℃ refrigerator to freeze, freeze-dry, and sieve to obtain safflower oil microcapsules. The average particle size, oil embedding rate and safflower oil melting temperature of the safflower oil microcapsules in the above-mentioned embodiment were measured. The results are shown in Table 1 below: Table 1 After comparison of comprehensive performance, the safflower seed oil microcapsules prepared in Example 3 were selected for subsequent experiments. Example 7 Preparation of salmon oil microcapsules: Take 1g of modified gelatin, dissolve it in 45g of deionized water, add 0.4g of salmon oil, homogenize at a speed of 15000rpm to obtain a crude salmon oil emulsion, ultrasonicate for 2.5min to obtain a salmon oil emulsion, put it in a -40℃ refrigerator to freeze, freeze-dry, and sieve to obtain salmon oil microcapsules. Example 8 Preparation of salmon oil microcapsules: Take 1g of modified gelatin, dissolve it in 45g of deionized water, add 0.6g of salmon oil, homogenize at a speed of 15000rpm to obtain a crude salmon oil emulsion, ultrasonicate for 2.5min to obtain a salmon oil emulsion, put it in a -40℃ refrigerator to freeze, freeze-dry, and sieve to obtain salmon oil microcapsules. Example 9 Preparation of salmon oil microcapsules: Take 1g of modified gelatin, dissolve it in 45g of deionized water, add 0.7g of salmon oil, homogenize at a speed of 15000rpm to obtain a crude salmon oil emulsion, ultrasonicate for 2.5min to obtain a salmon oil emulsion, put it in a -40℃ refrigerator to freeze, freeze-dry, and sieve to obtain salmon oil microcapsules. Example 10 Preparation of salmon oil microcapsules: Take 1g of modified gelatin, dissolve it in 45g of deionized water, add 0.6g of salmon oil, homogenize at a speed of 13500rpm to obtain a crude salmon oil emulsion, ultrasonicate for 2.5min to obtain a salmon oil emulsion, put it in a -40℃ refrigerator to freeze, freeze-dry, and sieve to obtain salmon oil microcapsules. Embodiment 11 Preparation of salmon oil microcapsules: Take 1g of modified gelatin, dissolve it in 45g of deionized water, add 0.6g of salmon oil, homogenize at a speed of 16000rpm to obtain a crude salmon oil emulsion, ultrasonicate for 2.5min to obtain a salmon oil emulsion, put it in a -40℃ refrigerator to freeze, freeze-dry, and sieve to obtain salmon oil microcapsules. Comparative Example 2 The difference between this embodiment and embodiment 8 is that unmodified gelatin is used, specifically as follows: Preparation of salmon oil microcapsules: Take 1g gelatin, dissolve it in 45g deionized water, add 0.6g salmon oil, homogenize at a speed of 15000rpm to obtain a crude salmon oil emulsion, ultrasonicate for 2.5min to obtain a salmon oil emulsion, put it into a -40℃ refrigerator to freeze, freeze-dry, and sieve to obtain salmon oil microcapsules. The average particle size, oil embedding rate and salmon oil melting temperature of the salmon oil microcapsules in the above example were measured. The results are shown in Table 2 below: Table 2 Average particle size (nm) Embedding rate (%) Salmon oil melting temperature (℃) Example 7 313.7 93.44 37.7 Example 8 315.6 94.13 37.8 Example 9 316.8 94.02 37.8 Example 10 322.1 93.67 37.7 Embodiment 11 302.2 94.05 37.8 Comparative Example 2 355.5 90.28 36.1 After comparison of comprehensive performance, the salmon oil microcapsules prepared in Example 8 were selected for subsequent experiments. Example 12 A method for preparing a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs comprises the following steps: (1) taking chitosan, dissolving it in 1% acetic acid solution, and adding glycerol to obtain a chitosan solution with a mass fraction of 3%, which is used as the cortex solution; (2) Take 1 g of safflower oil microcapsules, 3 g of salmon oil microcapsules and 50 mL of water, stir evenly, and use them as the core layer solution; (3) using ethanol as a coagulation bath, wet spinning the cortex solution and the core solution, with the cortex spinning flow rate being 50 mL / h and the core spinning flow rate being 80 mL / h, to obtain chitosan fibers coated with safflower seed oil microcapsules and salmon oil microcapsules; (4) Drying and chopping into pieces of 1-2 mm to obtain a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs. Example 13 A method for preparing a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs comprises the following steps: (1) taking chitosan, dissolving it in 1% acetic acid solution, and adding glycerol to obtain a chitosan solution with a mass fraction of 4%, which is used as the cortex solution; (2) Take 1 g of safflower oil microcapsules, 3 g of salmon oil microcapsules and 50 mL of water, stir evenly, and use them as the core layer solution; (3) using ethanol as a coagulation bath, wet spinning the cortex solution and the core solution, with the cortex spinning flow rate being 50 mL / h and the core spinning flow rate being 80 mL / h, to obtain chitosan fibers coated with safflower seed oil microcapsules and salmon oil microcapsules; (4) Drying and chopping into pieces of 1-2 mm to obtain a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs. Embodiment 14 A method for preparing a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs comprises the following steps: (1) taking chitosan, dissolving it in 1% acetic acid solution, and adding glycerol to obtain a chitosan solution with a mass fraction of 5%, which is used as the cortex solution; (2) Take 1 g of safflower oil microcapsules, 3 g of salmon oil microcapsules and 50 mL of water, stir evenly, and use them as the core layer solution; (3) using ethanol as a coagulation bath, wet spinning the cortex solution and the core solution, with the cortex spinning flow rate being 50 mL / h and the core spinning flow rate being 80 mL / h, to obtain chitosan fibers coated with safflower seed oil microcapsules and salmon oil microcapsules; (4) Drying and chopping into pieces of 1-2 mm to obtain a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs. Embodiment 15 A method for preparing a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs comprises the following steps: (1) taking chitosan, dissolving it in 1% acetic acid solution, and adding glycerol to obtain a chitosan solution with a mass fraction of 4%, which is used as the cortex solution; (2) Take 1 g of safflower oil microcapsules, 2 g of salmon oil microcapsules and 50 mL of water, stir evenly, and use them as the core layer solution; (3) using ethanol as a coagulation bath, wet spinning the cortex solution and the core solution, with the cortex spinning flow rate being 50 mL / h and the core spinning flow rate being 80 mL / h, to obtain chitosan fibers coated with safflower seed oil microcapsules and salmon oil microcapsules; (4) Drying and chopping into pieces of 1-2 mm to obtain a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs. Example 16 A method for preparing a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs comprises the following steps: (1) taking chitosan, dissolving it in 1% acetic acid solution, and adding glycerol to obtain a chitosan solution with a mass fraction of 4%, which is used as the cortex solution; (2) Take 1 g of safflower oil microcapsules, 4 g of salmon oil microcapsules and 50 mL of water, stir evenly, and use them as the core layer solution; (3) using ethanol as a coagulation bath, wet spinning the cortex solution and the core solution, with the cortex spinning flow rate being 50 mL / h and the core spinning flow rate being 80 mL / h, to obtain chitosan fibers coated with safflower seed oil microcapsules and salmon oil microcapsules; (4) Drying and chopping into pieces of 1-2 mm to obtain a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs. Comparative Example 3 The difference between this embodiment and embodiment 13 is that all safflower seed oil microcapsules are used, specifically as follows: A method for preparing a safflower seed oil preparation comprises the following steps: (1) taking chitosan, dissolving it in 1% acetic acid solution, and adding glycerol to obtain a chitosan solution with a mass fraction of 4%, which is used as the cortex solution; (2) Take 3 g of safflower seed oil microcapsules and 50 mL of water, stir evenly, and use them as the core layer solution; (3) using ethanol as a coagulation bath, wet spinning the cortex solution and the core solution, with the cortex spinning flow rate being 50 mL / h and the core spinning flow rate being 80 mL / h, to obtain chitosan fibers coated with safflower seed oil microcapsules; (4) Drying and cutting into pieces with a length of 1-2 mm to obtain safflower seed oil preparation. Comparative Example 4 The difference between this embodiment and embodiment 13 is that safflower seed oil / salmon oil mixed microcapsules are used, and the mass ratio of safflower seed oil / salmon oil is consistent with that of embodiment 13, specifically as follows: A method for preparing a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs comprises the following steps: (1) taking chitosan, dissolving it in 1% acetic acid solution, and adding glycerol to obtain a chitosan solution with a mass fraction of 4%, which is used as the cortex solution; (2) Take 4 g of safflower oil / salmon oil mixed microcapsules and 50 mL of water, stir evenly, and use them as the core layer solution; (3) using ethanol as a coagulation bath, wet spinning the cortex solution and the core solution, with the cortex spinning flow rate being 50 mL / h and the core spinning flow rate being 80 mL / h, to obtain chitosan fibers coated with safflower seed oil microcapsules and salmon oil microcapsules; (4) Drying and chopping into pieces of 1-2 mm to obtain a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs. Preparation of safflower oil / salmon oil mixed microcapsules: take modified gelatin, dissolve it in deionized water, add safflower oil and salmon oil, homogenize at a speed of 14500 rpm to obtain safflower oil / salmon oil crude emulsion, ultrasonicate for 2.5 minutes to obtain safflower oil emulsion, put it into a -40°C refrigerator to freeze, freeze-dry, and sieve to obtain safflower oil / salmon oil microcapsules. Determine the ratio of ω-6 unsaturated fatty acids to ω-3 polyunsaturated fatty acids in the safflower seed oil compound preparations for improving skin inflammation in pet cats and dogs obtained in the above-mentioned embodiments; (1) Determine the oxidative stability index (OSI): use a fat oxidation stability tester to determine the temperature at 110°C and the airflow rate at 20L / h; (2) Determine the initial DPPH free radical scavenging rate: take 0.2g of oil sample and mix it with 0.75mL of methanol, vortex and oscillate for 1min, and take the supernatant to be tested. Take 0.21mL of the test solution, add 0.7mL of DPPH-ethanol solution (0.1mmol / L), vortex and mix, react at 25°C in the dark for 30min, and measure the absorbance at 517nm; (3) Determine the initial ABTS free radical scavenging rate: take 0.30mL of the test solution, add 0.18μL of working solution (composed of 7mmol / L ABTS solution and 2.45mmol / LK 2 S2O8 solution was prepared), reacted at 25°C in the dark for 30 minutes, and the absorbance was measured at 734 nm; the results are shown in Table 3 below: Table 3 It can be seen from Comparative Example 4 in the above table that after safflower seed oil and salmon oil are mixed, their oxidative stability index, DPPH free radical scavenging rate and ABTS free radical scavenging rate are all reduced. Therefore, early mixing of oils and fats will accelerate oxidation. Therefore, early mixing is not recommended. The storage stability of Example 12, Example 13 and Comparative Example 4 was measured. Each example was stored at 20-25° C., and the dissolution time of safflower oil and salmon oil from the microcapsule and the dissolution time from the fiber were measured at different times. The results are shown in Table 4 below. Table 4 The peroxide value (POV value) of Example 13 and Comparative Example 4 was measured. The peroxide value was measured according to GB 5009.227-2023 Determination of peroxide value of animal and vegetable oils. In Example 13, before the oil was dissolved from the microcapsule, the safflower seed oil and salmon oil were measured separately. After the oil was dissolved from the microcapsule, the mixed oil was measured. The results are as follows Figure 1 shown. A study on the therapeutic effect of safflower seed oil compound preparation on improving skin inflammation in pet cats and dogs: 24 sick dogs and cats were diagnosed with skin diseases in an animal hospital with clinical manifestations of hair loss, erythema, scabs, papules, etc., including 12 dogs and 12 cats. Dogs and cats were divided into 3 groups, 8 in each group, and the safflower seed oil compound preparations for improving skin inflammation in pet cats and dogs prepared in Example 13, Comparative Example 3 and Comparative Example 4 were added to the daily diet. The dosage for cats was 0.88 g / kg body weight / d, and the dosage for dogs was 0.92 g / kg body weight / d, and the feeding was continued for 30 days. Establishment of a scoring standard for the severity of clinical symptoms in dogs and cats: The 24 dogs and cats participating in the study were scored for the severity of skin lesions by three veterinary staff before and after treatment, and the average of the three numbers was used as the skin lesion score. The specific scoring method was: for 16 different body parts (Table 5), the severity of hair loss, erythema, scabs and papules was evaluated using a grading system of 0 points for no symptoms, 1 point for mild symptoms, 2 points for moderate symptoms and 3 points for severe symptoms. Table 5 The pets involved in the experiment included 6 female dogs, 6 male dogs, 6 female cats and 6 male cats. 75% of the pets had hair loss symptoms, 54% had erythema, 50% had scabs and 42% had papules. The skin lesion scores before and after treatment are shown in Table 6 below, wherein No. 1-8 uses the safflower seed oil compound preparation for improving skin inflammation of pet cats and dogs prepared in Example 13; No. 9-16 uses the safflower seed oil preparation prepared in Comparative Example 3, and No. 17-24 uses the safflower seed oil compound preparation for improving skin inflammation of pet cats and dogs prepared in Comparative Example 4: Table 6 Efficacy evaluation criteria: Establish the efficacy evaluation criteria for erythema, hair loss, papules and scabs. The efficacy index (E) is the score before treatment - the score after treatment / the score before treatment × 100%. Efficacy index: 100% ≥ E > 75% is excellent; 75% ≥ E > 50% is good; 50% ≥ E > 25% is fair; 25% ≥ E is ineffective. The efficacy evaluation is shown in Table 7 below: Table 7 Adverse reaction record: The condition of dogs and cats was observed daily and the number of cases with symptoms such as vomiting, diarrhea, lethargy, loss of appetite, polydipsia, and polyuria during treatment was recorded. Among the 24 sick dogs and cats participating in the trial, cat No. 14 had slight vomiting symptoms, and the other sick dogs and cats showed no adverse reactions during the treatment.

Claims

1. A safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs, characterized in that: The safflower seed oil compound preparation for improving skin inflammation of pet cats and dogs is a fiber with safflower seed oil microcapsules and deep-sea fish oil microcapsules loaded inside; the material of the fiber is a pH-responsive material, and the membrane of the microcapsule is a temperature-responsive membrane.

2. A safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs according to claim 1, characterized in that: The pH response value of the pH responsive material is ≤5.

3. A safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs according to claim 1, characterized in that: The temperature response value of the temperature responsive membrane is ≥37.7°C.

4. A safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs according to claim 1, characterized in that: The deep sea fish oil includes salmon oil, sardine oil, tuna oil and mackerel oil.

5. A method for preparing a safflower seed oil composite preparation for improving skin inflammation in pet cats and dogs according to any one of claims 1 to 4, characterized in that: The following steps are involved: (1) Preparation of safflower oil microcapsules: Take modified gelatin, dissolve it in deionized water, add safflower oil, homogenize at a speed of 12000-15000 rpm to obtain a safflower oil crude emulsion, ultrasonicate for 2-3 min to obtain a safflower oil emulsion, freeze it in a -40°C refrigerator, freeze-dry it, and sieve it to obtain safflower oil microcapsules; (2) Preparation of deep-sea fish oil microcapsules: Take modified gelatin, dissolve it in deionized water, add deep-sea fish oil, homogenize at a speed of 13500-16000 rpm to obtain a deep-sea fish oil crude emulsion, ultrasonicate for 2-3 min to obtain a deep-sea fish oil emulsion, freeze it in a -40°C refrigerator, freeze-dry it, and sieve it to obtain deep-sea fish oil microcapsules; (3) taking chitosan, dissolving it in 1% acetic acid solution, and adding glycerol to obtain a chitosan solution with a mass fraction of 3-5%, which is used as the cortex solution; (4) taking safflower seed oil microcapsules, deep-sea fish oil microcapsules and water, and stirring them evenly to prepare a core layer solution; (5) using ethanol as a coagulation bath, wet spinning the cortex solution and the core solution, with the cortex spinning flow rate being 50 mL / h and the core spinning flow rate being 80 mL / h, to obtain chitosan fibers coated with safflower seed oil microcapsules and deep-sea fish oil microcapsules; (6) Drying and chopping into pieces of 1-2 mm to obtain a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs.

6. The method for preparing a safflower seed oil composite preparation for improving skin inflammation of pet cats and dogs according to claim 5, characterized in that: The preparation method of the modified gelatin is as follows: Step 1: prepare a gelatin aqueous solution with a concentration of 20 mg / mL and adjust the pH to 9.00; Step 2: preparing a grape seed polyphenol aqueous solution with a concentration of 4 mg / mL and adjusting the pH to 9.00; Step 3: Mix the gelatin aqueous solution and the grape seed polyphenol aqueous solution in equal volumes, and stir at 36°C and 200-300 rpm for 20-24 hours; Step 4: Place in a dialysis bag with a molecular weight cutoff of 8000-14000Da and dialyze for 48 hours, replacing ultrapure water every 6 hours; Step 5: Freeze-dry to obtain modified gelatin.

7. The method for preparing a safflower seed oil compound preparation for improving skin inflammation of pet cats and dogs according to claim 5, characterized in that: In the step (1), the mass ratio of modified gelatin, deionized water and safflower seed oil is 1:50:(0.5-0.8).

8. The method for preparing a safflower seed oil composite preparation for improving skin inflammation in pet cats and dogs according to claim 5, characterized in that: In the step (2), the mass ratio of modified gelatin, deionized water and deep-sea fish oil is 1:45:(0.4-0.7).

9. The method for preparing a safflower seed oil composite preparation for improving skin inflammation of pet cats and dogs according to claim 5, characterized in that: In the step (4), the ratio of safflower seed oil microcapsules to deep-sea fish oil microcapsules is 1:(3-5).

10. Use of the safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs according to any one of claims 1 to 4 in anti-inflammatory products for pet cats and dogs.

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