Composition for dispelling effects of alcohol and protecting liver and preparation method thereof

Abstract

The invention provides a composition for dispelling effects of alcohol and protecting liver and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine compositions. The alcohol effect dispelling and liver protecting composition is prepared from the following raw materials in parts by weight: 10 to 20 parts of puerarin, 10 to 20 parts of curcumin, 5 to 10 parts of dihydromyricetin, 1 to 5 parts of piperine, 0.05 to 0.3 part of trace elements and 0.05 to 0.3 part of vitamins. Effective substances are coated and protected, so that the biocompatibility is improved, the metabolism is reduced, and various forms of preparations can be prepared and are convenient to take; tests prove that the drunkenness time can be shortened by 50% or above, and the traditional Chinese medicine composition has an obvious treatment effect on alcoholic liver injury.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine compositions, and particularly relates to a hangover-relieving and liver-protecting composition and a preparation method thereof. Background Art

[0002] Alcoholic liver disease (ALD) is a liver disease caused by long-term heavy drinking, including four major categories: alcoholic fatty liver (AFL), alcoholic hepatitis (AH), alcoholic liver fibrosis (AHF) and alcoholic cirrhosis (AC). Currently, nutritional support and drug treatment are usually used. Patients with alcoholic liver damage generally lack vitamin B, vitamin C, vitamin E, etc., and they can supplement appropriate amounts of vitamins while improving their diet. Drug treatment mainly includes anti-inflammatory, liver-protecting and anti-fibrosis drugs. At present, modern medicine mainly treats alcoholic liver damage with abstinence and symptomatic treatment. So far, there is still a lack of drugs with definite efficacy that can be recommended for alcoholic liver damage.

[0003] In the treatment of alcoholic liver disease, TCM can treat alcoholic liver disease by syndrome differentiation and staging, or by empirical prescriptions or self-made prescriptions, which is quite effective. At present, the drugs used in the treatment of alcoholic liver damage by TCM mainly focus on blood circulation and stasis-removing drugs, diuretic and dampness-inducing drugs, antipyretic and heat-clearing drugs, qi-regulating and qi-tonifying drugs, and alcohol-detoxifying drugs. Although the side effects are small, there are problems such as inconsistent effects and long cycles.

[0004] Therefore, there is an urgent need for a composition which is easy to take, has a good alcohol sobering effect, and has a therapeutic and maintenance effect on the liver. Summary of the invention

[0005] In view of this, the object of the present invention is to provide a hangover-relieving and liver-protecting composition, wherein the prepared hangover-relieving composition has an obvious hangover-relieving function and has an obvious therapeutic effect on alcoholic liver damage.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] The present invention provides a hangover-relief and liver-protection composition, which is prepared from the following raw materials in parts by weight:

[0008] 10-20 parts of puerarin, 10-20 parts of curcumin, 5-10 parts of dihydromyricetin, 1-5 parts of piperine, 0.05-0.3 parts of trace elements, and 0.05-0.3 parts of vitamins.

[0009] Usually, Chinese medicine preparations are directly extracted from kudzu root and turmeric to obtain extracts, but the content of effective ingredients in the extracts in this method is difficult to control, so the efficacy varies. The present invention uses further extracted effective substances to accurately mix the obtained composition with stable effect.

[0010] Puerarin and curcumin are both active ingredients in plant extracts. Curcumin can scavenge free radicals and reduce the damage of oxidative stress to liver cells. The anti-inflammatory effect of curcumin can reduce liver inflammation, and puerarin may help reduce inflammation by regulating immune response. Dihydromyricetin is a natural flavonoid compound that promotes the metabolism of ethanol by accelerating the decomposition of acetaldehyde, a product of ethanol metabolites. In the human body, ethanol is first converted into acetaldehyde by alcohol dehydrogenase (ADH), and then acetaldehyde is further oxidized into harmless acetic acid by acetaldehyde dehydrogenase (ALDH2), and finally metabolized into water and carbon dioxide and excreted from the body. Dihydromyricetin can protect the liver and accelerate the rapid decomposition of acetaldehyde, thereby reducing the damage of acetaldehyde to liver cells. Piperine can enhance the permeability of cell membranes, inhibit the efflux of multiple drug transporters, and reduce the metabolic effects of co-administered drugs, thereby enhancing the absorption and efficacy of drugs.

[0011] Preferably, the trace elements are one or more of zinc ions, iron ions, magnesium ions, selenium ions, copper ions, and manganese ions. The present invention finds that patients with alcoholic liver disease are usually deficient in trace elements, among which the role of zinc ions in sobering up and protecting the liver is mainly reflected in its participation in the activity of multiple enzymes, which are essential for the metabolism of alcohol and the protective function of the liver. Zinc α2 glycoprotein has been found to inhibit the activation and proliferation of hepatic stellate cells, thereby reversing liver fibrosis to a certain extent. In addition, zinc is also associated with antioxidant, anti-inflammatory and anti-apoptotic effects; while supplementing other trace elements is beneficial to promoting the improvement and recovery of liver function.

[0012] Preferably, the vitamin is one or more of vitamin C, vitamin A, vitamin D, vitamin E, vitamin B6, vitamin B12, thiamine, and riboflavin. The present invention finds that long-term and heavy drinking may change liver vitamin metabolism, leading to vitamin deficiency, which may be related to the development of alcoholic liver damage; for example, vitamin C plays multiple roles in the body, especially in protecting cells from oxidative stress damage. During alcohol metabolism, the liver produces a large number of free radicals, which can attack cell membranes, proteins and DNA, leading to cell damage and oxidative stress. Vitamin C can neutralize these free radicals by donating electrons, thereby reducing their destructive effects on liver cells; thiamine is a component of the pyruvate decarboxylase (PDC) complex, which is responsible for catalyzing the reaction of pyruvate into acetaldehyde. Thiamine participates in this reaction in the form of its phosphorylated form thiamine pyrophosphate (TPP), helping to remove the carbonyl group of pyruvate to generate acetaldehyde, which is then converted into ethanol under the action of alcohol dehydrogenase (ADH). Supplementing an appropriate amount of vitamins can effectively improve the liver state.

[0013] The present invention also provides a method for preparing the alcohol sobering and liver protecting composition, comprising the following steps:

[0014] S1. Weigh the raw materials according to the weight parts;

[0015] S2. Dissolving curcumin, puerarin, dihydromyricetin and piperine in an ethanol solution to obtain a drug mixture;

[0016] S3. The glycyrrhizic acid solution was dripped into the drug mixture while stirring, and then the solvent was removed in vacuo to obtain a pre-coated powder;

[0017] S4. Dissolve the pre-coated powder, trace elements, vitamins, and plant protein hydrolyzate in deionized water, stir thoroughly, and then freeze-dry to obtain a hangover and liver protection composition.

[0018] Glycyrrhizic acid is the main active ingredient of licorice. It has surfactant properties and can spontaneously form micelles in aqueous solution to solubilize poorly soluble components. It also has the functions of promoting drug absorption, reducing the toxic side effects of chemotherapy and reversing drug resistance. It can also protect the action time of effective Chinese herbal ingredients in the body, reduce metabolic waste, and thus improve the utilization rate of Chinese herbal ingredients. At the same time, the use of plant protein hydrolysate to assist in coating the effective ingredients further increases the stability of the effect and improves the shelf life.

[0019] Preferably, the mass ratio of curcumin, puerarin, dihydromyricetin, piperine to the ethanol solution in S2 is 1-3:10.

[0020] Preferably, the concentration of the ethanol solution is 50-80%.

[0021] Preferably, the stirring rate in S3 is 10-15 r / min.

[0022] Preferably, the concentration of the glycyrrhizic acid solution in S3 is 10-20wt%, and the mass ratio of glycyrrhizic acid in the glycyrrhizic acid solution to curcumin in the drug mixture is 1:1.

[0023] Preferably, the mass ratio of the plant protein hydrolysate to the pre-coated powder is 1:1-1.5.

[0024] Preferably, the alcohol-relieving and liver-protecting composition can be prepared into a drink, tablet or granule.

[0025] Contains at least the following beneficial technical effects:

[0026] 1. The alcohol sobering composition prepared by the present invention has obvious alcohol sobering function, and experiments have proved that it can shorten the drunkenness time by more than 50%.

[0027] 2. The alcohol-detoxifying composition prepared by the present invention has an obvious therapeutic effect on alcoholic liver damage, has a long action time in the body, and has good biocompatibility. DETAILED DESCRIPTION

[0028] The technical solution of the present invention is further described below.

[0029] Unless otherwise defined, technical or scientific terms used in the present invention shall have the common meanings understood by one having ordinary skills in the field to which the present invention belongs.

[0030] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the present invention.

[0031] In addition, it should be understood that although this specification is described according to the implementation modes, not every implementation mode includes only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation modes that can be understood by those skilled in the art. These other implementation modes are also covered within the protection scope of the present invention.

[0032] It should also be understood that the specific embodiments described above are only used to explain the present invention, and the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, can make equivalent replacements or changes based on the technical solutions and inventive concepts of the present invention, which should be covered by the protection scope of the present invention / invention.

[0033] The raw materials used in the following examples are all commercially available.

[0034] Example 1

[0035] This embodiment provides a method for preparing a hangover-relieving and liver-protecting composition, the steps are as follows:

[0036] S1. Weigh 15 parts of puerarin, 15 parts of curcumin, 8 parts of dihydromyricetin, 3 parts of piperine, trace elements, and vitamins by weight;

[0037] The trace elements are composed of: 0.05 parts of zinc ions, 0.05 parts of iron ions, 0.05 parts of magnesium ions, 0.05 parts of selenium ions, 0.05 parts of copper ions, and 0.05 parts of manganese ions.

[0038] The vitamin composition comprises: 0.05 part of vitamin C, 0.01 part of vitamin A, 0.01 part of vitamin D, 0.01 part of vitamin E, 0.01 part of vitamin B6, 0.01 part of vitamin B12, 0.05 part of thiamine and 0.05 part of riboflavin.

[0039] S2. Dissolve curcumin, puerarin, dihydromyricetin and piperine in 5 times the mass of ethanol solution to obtain a drug mixture;

[0040] S3. 15 parts by weight of glycyrrhizic acid was prepared into a glycyrrhizic acid solution and then dropped into the drug mixture at a stirring rate of 15r / min, and then the solvent was removed in vacuo to obtain a pre-coated powder;

[0041] S4. Dissolve the pre-coated powder, trace elements, vitamins and plant protein hydrolysate in deionized water, stir thoroughly and freeze-dry to obtain a hangover and liver protection composition; wherein the mass ratio of the plant protein hydrolysate to the pre-coated powder is 1:1.

[0042] S5. Add auxiliary materials to prepare a drink.

[0043] Example 2

[0044] This embodiment provides a method for preparing a hangover-relieving and liver-protecting composition, the steps are as follows:

[0045] S1. Weigh 10 parts of puerarin, 10 parts of curcumin, 5 parts of dihydromyricetin, 1 part of piperine, trace elements, and vitamins by weight.

[0046] The trace element composition is: 0.05 parts of zinc ions.

[0047] The vitamin composition is: 0.05 parts of vitamin C.

[0048] S2. Dissolve curcumin, puerarin, dihydromyricetin and piperine in 3 times the mass of ethanol solution to obtain a drug mixture;

[0049] S3. 10 parts of glycyrrhizic acid were prepared into a glycyrrhizic acid solution and then dropped into the drug mixture at a stirring rate of 10r / min, and then the solvent was removed in vacuo to obtain a pre-coated powder;

[0050] S4. The pre-coated powder, trace elements, vitamins, and plant protein hydrolysate were dissolved in deionized water, stirred thoroughly, and freeze-dried to obtain a hangover and liver protection composition; wherein the mass ratio of the plant protein hydrolysate to the pre-coated powder was 1:1;

[0051] S5. Add auxiliary materials to prepare a drink.

[0052] Example 3

[0053] This embodiment provides a method for preparing a hangover-relieving and liver-protecting composition, the steps are as follows:

[0054] S1. Weigh 20 parts of puerarin, 20 parts of curcumin, 10 parts of dihydromyricetin, 5 parts of piperine, trace elements, and vitamins by weight.

[0055] The trace element composition is: 0.05 parts of zinc ions, 0.05 parts of iron ions, 0.05 parts of magnesium ions, and 0.05 parts of selenium ions.

[0056] The vitamin composition is: 0.05 part of vitamin C, 0.01 part of vitamin A, 0.01 part of vitamin D, 0.01 part of vitamin E, 0.01 part of vitamin B6, and 0.01 part of vitamin B12.

[0057] S2. Dissolve curcumin, puerarin, dihydromyricetin and piperine in 10 times the mass of ethanol solution to obtain a drug mixture;

[0058] S3. 10-20 parts of glycyrrhizic acid were prepared into a glycyrrhizic acid solution and then dropped into the drug mixture at a stirring rate of 15r / min, and then the solvent was removed in vacuo to obtain a pre-coated powder;

[0059] S4. The pre-coated powder, trace elements, vitamins, and plant protein hydrolysate were dissolved in deionized water, stirred thoroughly, and freeze-dried to obtain a hangover and liver protection composition; wherein the mass ratio of the plant protein hydrolysate to the pre-coated powder was 1:1.5;

[0060] S5. Add auxiliary materials to prepare a drink.

[0061] Comparative Example 1

[0062] This comparative example is prepared in the same manner as Example 1, except that dihydromyricetin is not contained.

[0063] Comparative Example 2

[0064] This comparative example is prepared in the same manner as in Example 1, except that piperine is not contained.

[0065] Comparative Example 3

[0066] This comparative example provides a method for preparing a hangover-relieving and liver-protecting composition:

[0067] Weigh 20 parts of puerarin, 20 parts of curcumin, 10 parts of dihydromyricetin, 5 parts of piperine, 0.05 parts of zinc ion, 0.05 parts of iron ion, 0.05 parts of magnesium ion, 0.05 parts of selenium ion, 0.05 parts of copper ion, 0.05 parts of manganese ion, 0.05 parts of vitamin C, 0.01 parts of vitamin A, 0.01 parts of vitamin D, 0.01 parts of vitamin E, 0.01 parts of vitamin B6, 0.01 parts of vitamin B12, 0.05 parts of thiamine, and 0.05 parts of riboflavin by weight, and mix well.

[0068] Experimental Example 1

[0069] The alcohol sobering and liver protecting compositions prepared in Examples 1-3 and Comparative Examples 1-3 were used on drunken mice, and the sobering time of the mice was counted.

[0070] 70 female mice weighing 20-30 g were randomly divided into a control group, Example 1-3 groups and Comparative Example 1-3 groups with 10 mice in each group. The mice were fasted for 10 hours before the experiment. According to the weight of the mice, each group was gavaged with 53% ethanol (20 ml / kg); 30 minutes later, the mice in each group were given the drug at the same time;

[0071] The blank group was given distilled water, the Example 1-3 groups were given the compositions prepared in Examples 1-3 respectively; the comparative examples were given the compositions prepared in Comparative Examples 1-3 respectively; the dosage was calculated based on the body weight of the mice, 5 g / kg.

[0072] The activities of mice in each group after drinking were observed. Unsteady crawling, closed eyes and motionless with limbs facing upward were considered as indicators of drunkenness (loss of righting reflex), and free movement and good spirits were considered as indicators of not being drunk. The duration of drunkenness was calculated. The results are shown in Table 1.

[0073] Table 1

[0074]

[0075]

[0076] As shown in Table 1, the compositions obtained in Examples 1-3 of the present invention can shorten the time of drunkenness and have the function of sobering up. The sobering up function of Comparative Examples 1-2 is reduced after removing some raw materials. The sobering up ability of Comparative Example 3 is significantly weakened because the components are not fully utilized due to the metabolic process, resulting in poor sobering up ability.

[0077] Experimental Example 2

[0078] 80 female mice weighing 20-30 g were randomly divided into a blank group, a control group, example 1-3 groups and comparative example 1-3 groups, with 10 mice in each group.

[0079] All mice were fed normally for 10 days, and then the blank group continued to be fed normally. The control group, Example 1-3 groups and Comparative Example 1-3 groups were gavaged with 53% ethanol (10 ml / kg) every day after the mice had eaten. After 15 days, the gavage of ethanol to the mice was stopped to obtain model mice.

[0080] The model mice of the control group, Example 1-3 group and Comparative Example 1-3 group were treated with medication respectively; the control group was gavaged with 5 g / kg distilled water daily; the Example 1-3 group was gavaged with 5 g / kg of the decoction obtained in Example 1-3; the Comparative Example 1-3 group was gavaged with 5 g / kg of the decoction obtained in Comparative Example 1-3; the mice were killed after continuous gavage for 7 days, and the whole liver was taken out to prepare 10% liver homogenate, and the contents of TG, MDA and GSH were determined, as shown in Table 2.

[0081] Table 2

[0082]

[0083]

[0084] It can be seen from Table 2 that the hangover-relieving compositions prepared in Examples 1-3 of the present invention have obvious therapeutic and protective effects on liver damage caused by long-term drinking, and can treat long-term alcoholic liver damage.

[0085] The above description is only the preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A composition for sobering up and protecting the liver, characterized in that: Prepared from the following raw materials in parts by weight: 10-20 parts of puerarin, 10-20 parts of curcumin, 5-10 parts of dihydromyricetin, 1-5 parts of piperine, 0.05-0.3 parts of trace elements, and 0.05-0.3 parts of vitamins.

2. The alcohol sobering and liver protecting composition according to claim 1, characterized in that: The trace elements are one or more of zinc ions, iron ions, magnesium ions, selenium ions, copper ions, and manganese ions.

3. The alcohol sobering and liver protecting composition according to claim 1, characterized in that: The vitamins are: one or more of vitamin C, vitamin A, vitamin D, vitamin E, vitamin B6, vitamin B12, thiamine, and riboflavin.

4. The method for preparing the alcohol sobering and liver protecting composition according to any one of claims 1 to 3, characterized in that: The following steps are involved: S1. Weigh the raw materials according to the weight parts; S2. Dissolving curcumin, puerarin, dihydromyricetin and piperine in an ethanol solution to obtain a drug mixture; S3. The glycyrrhizic acid solution was dripped into the drug mixture while stirring, and then the solvent was removed in vacuo to obtain a pre-coated powder; S4. Dissolve the pre-coated powder, trace elements, vitamins, and plant protein hydrolyzate in deionized water, stir thoroughly, and then freeze-dry to obtain a hangover and liver protection composition.

5. The preparation method according to claim 4, characterized in that: The mass ratio of curcumin, puerarin, dihydromyricetin, piperine and ethanol solution in S2 is 1-3:

10.

6. The preparation method according to claim 5, characterized in that: The concentration of the ethanol solution is 50-80%.

7. The preparation method according to claim 4, characterized in that: The stirring rate in S3 is 10-15 r / min.

8. The preparation method according to claim 4, characterized in that: The concentration of the glycyrrhizic acid solution in S3 is 10-20wt%, and the mass ratio of glycyrrhizic acid in the glycyrrhizic acid solution to curcumin in the drug mixture is 1:

1.

9. The preparation method according to claim 4, characterized in that: The mass ratio of the plant protein hydrolysate to the pre-coated powder is 1:1-1.

5.

10. The alcohol sobering and liver protecting composition according to claim 1, characterized in that: The alcohol-relieving and liver-protecting composition can be prepared into a drink, tablet or granule.

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