Preparation method and application of large-particle colloidal gold
By controlling the temperature and rotation speed in the reactor through the dual reducing agent method, large-particle colloidal gold with uniform particle size was prepared, which solved the problems of complex process and low preparation efficiency in the prior art, and achieved high sensitivity detection and improvement of production efficiency.
CN119681276AActive Publication Date: 2025-03-25HUNAN QIANKANG TECH CO LTD
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Patent Information
- Application Number
- CN202411488138.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-24
- Publication Date
- 2025-03-25
- Estimated Expiration
- 2044-10-24
AI Technical Summary
Technical Problem
The existing immunocolloidal gold technology is complex and unstable when preparing large-particle colloidal gold, which is difficult to meet the needs of high-sensitivity detection, and is inefficient in preparation and is difficult to mass produce.
Method used
The dual-reducing agent method is adopted to prepare large-grain colloidal gold with uniform particle size by strictly controlling the temperature and rotation speed in the reactor, and using ascorbic acid and trisodium citrate as reducing agents.
Benefits of technology
It realizes stable preparation of large-particle colloidal gold with uniform particle size, simplifies the process, improves production efficiency, and greatly improves sensitivity, and is suitable for high-sensitivity detection.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention discloses a preparation method and application of large-particle colloidal gold, and the method comprises the following steps: (1) pouring double distilled water into a container, adding a gold chloride solution, and stirring at the rotating speed of 600-800rpm / min for 3-7min; (2) adding ascorbic acid with the concentration of 0.8-1.2 M, and continuously stirring for 15-25 minutes; (3) adding a trisodium citrate solution, heating and stirring until boiling, continuously stirring for 3-7 minutes, stopping heating, standing and cooling to obtain a colloidal gold solution; and (4) after the colloidal gold solution is cooled, pouring the colloidal gold solution into a clean brown light-proof bottle, ensuring that the volume of the obtained colloidal gold solution is consistent with the volume of the initial double distilled water in the step (1), adding Proclin300 into the colloidal gold solution, detecting an absorption peak and absorbance by using a spectrophotometer, and preserving in a dark place.
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