Microbial incubator and working method thereof

Abstract

The invention relates to the technical field of incubators, in particular to a microbial incubator and a working method thereof.The microbial incubator comprises an incubator body, a culture chamber, a humidifying mechanism and a constant-temperature mechanism; a culture chamber is formed in the box body; a bearing frame I and a bearing frame II are movably arranged in the culture room, and a plurality of culture dish placing positions are arranged on the bearing frame I and the bearing frame II; a humidifying mechanism is mounted at the bottom of the culture room, and the working end of the humidifying mechanism extends into the culture room; the working end of the constant-temperature mechanism penetrates through the interior of the culture chamber. Through cooperative arrangement of the humidifying mechanism and the constant-temperature mechanism, temperature and humidity control of the microbial incubator is realized, constant-temperature and constant-humidity culture of microorganisms is facilitated, and the accuracy of experimental data is ensured.

Description

Technical Field

[0001] The invention relates to the technical field of incubators, in particular to a microbial incubator and a working method thereof. Background Art

[0002] A microbial incubator is a device used to culture and reproduce microorganisms. It provides a closed environment suitable for the growth of microorganisms. The main function of a microbial incubator is to provide a controllable microbial culture environment for scientific research laboratories, medical laboratories, industrial production and other places to meet the growth and reproduction needs of different microorganisms.

[0003] At present, some existing microbial incubators cannot regulate the temperature and humidity inside the incubator well, which makes the temperature and humidity inside the incubator easily affected by the external environment and fluctuate. Studies have shown that too high temperature can cause protein denaturation or cell membrane structure damage of microorganisms, too low temperature can slow down the metabolism rate and growth rate of microorganisms, and too high humidity can cause condensation of water droplets in the air to pollute the environment, while too low humidity can cause the air to dry out, which seriously affects the growth and reproduction of microorganisms and affects the experimental results. Summary of the invention

[0004] In view of the shortcomings of the prior art, the present invention provides a microbial incubator and a working method thereof. Through the coordinated setting of a humidification mechanism and a constant temperature mechanism, the temperature and humidity control of the microbial incubator is achieved, which is more conducive to constant temperature and humidity cultivation of microorganisms and ensures the accuracy of experimental data.

[0005] In order to achieve the above-mentioned purpose, the present invention provides a microorganism culture box, including a box body, a culture chamber, a humidifying mechanism and a constant temperature mechanism; the box body is provided with a culture chamber; the culture chamber is provided with a support frame 1 and a support frame 2, each of which is provided with a plurality of culture dish placement positions; a humidifying mechanism is installed at the bottom of the culture chamber, and the working end of the humidifying mechanism extends into the culture chamber; the working end of the constant temperature mechanism runs through the interior of the culture chamber. Through the coordinated arrangement of the box body, the culture chamber, the humidifying mechanism and the constant temperature mechanism, the humidifying mechanism can continuously provide the culture chamber with the specific humidity required for the growth and reproduction of microorganisms, and the constant temperature mechanism runs through the culture chamber to provide the culture chamber with a uniform and stable microorganism culture temperature, thereby ensuring the constant temperature and humidity culture conditions of the microorganisms.

[0006] Furthermore, the humidification mechanism includes a water tank, which is fixedly installed at the bottom of the culture chamber; a water level observation window is provided in the middle of the front side of the water tank, and a water injection port is provided on the upper front side of the water tank so as to be openable and closable. Through the coordinated arrangement of the water tank, the water level observation window and the water injection port, the water level observation window can be a transparent glass window, and the experimenter can check the water level in the water tank through the water level observation window. When the water level is low, the experimenter can complete the water injection action by opening the water injection port. The operation is simple and convenient, ensuring that the humidification mechanism can continuously provide a constant humidity environment for the culture chamber.

[0007] Furthermore, water delivery pump bodies are fixedly installed on both sides of the bottom of the water tank; the output ends of the two water delivery pump bodies are respectively connected to water delivery pipes; the two water delivery pipes are arranged opposite to each other and extend upward into the culture chamber. The two water delivery pipes are arranged upward and extend into the culture chamber to provide the culture chamber with the humidity environment required for microbial culture.

[0008] Furthermore, the water pipe inside the culture chamber is connected to the shunt pipe 1 and the shunt pipe 2 in sequence; the outlet end of the shunt pipe 1 is connected to the atomizing nozzle 1, and the outlet end of the shunt pipe 2 is connected to the atomizing nozzle 2. The shunt pipe 1 transports the water in the water tank to the atomizing nozzle 1 through the water pipe, and the atomizing nozzle 1 provides a suitable humidity environment for the culture dish on the support frame 1; the shunt pipe 2 transports the water in the water tank to the atomizing nozzle 2 through the water pipe, and the atomizing nozzle 2 provides a suitable humidity environment for the culture dish on the support frame 2, ensuring the constant humidity of the culture chamber.

[0009] Furthermore, the constant temperature mechanism includes a hot air blower, a circulating air blower and a circulating channel; the hot air blower is fixedly installed at the top of the outer side of the box; the circulating air blower is fixedly installed at the top of the inner side of the box; the circulating channel runs through the inner wall of the culture chamber; the output end of the hot air blower is connected to the circulating channel, and the output end of the circulating air blower is connected to the circulating channel. The heat generated by the hot air blower enters the inner side of the circulating air blower, and the circulating air blower performs cyclic heating and then transports the heat to the culture chamber through the circulating channel.

[0010] Furthermore, the inner wall of the culture chamber is provided with two or more air inlets, and an air outlet is arranged opposite to the air inlet; the air inlet and the air outlet are both arranged on the circulation channel. The air inlet sends hot air into the culture chamber, and the air inlet and the air outlet are arranged opposite to each other, which is conducive to convection of hot air.

[0011] Furthermore, a heat transfer plate 1 is fixedly connected to the lower part of the support frame 1, and a heat transfer plate 2 is fixedly connected to the lower part of the support frame 2; the heat transfer plate 1 and the heat transfer plate 2 are both connected to the circulation channel. Through the coordinated arrangement of the heat transfer plate 1 and the heat transfer plate 2, the culture dishes on the support frame 1 and the support frame 2 can obtain a uniform and stable temperature, which is conducive to the constant temperature culture of microorganisms.

[0012] Furthermore, an adjustable lighting lamp is arranged in the middle of the top of the culture chamber, and the adjustable lighting lamp can be used to control the lighting variables of microorganism culture.

[0013] Furthermore, the outer side of the box is hinged with a door, and a transparent window is arranged inside the door. The transparent window can observe the growth of microorganisms inside the culture chamber in real time without the need for the experimenter to open the door, effectively avoiding the exchange of temperature and humidity conditions inside the culture chamber with the external environment.

[0014] A control console is arranged at the bottom of the humidifying mechanism, and a power switch and a control panel are arranged on the control console.

[0015] Furthermore, a working method of a microbial incubator comprises the following steps: S1. Turn on the power switch of the incubator, turn on the hot air blower and circulating air blower, turn on the humidification mechanism, and set appropriate culture temperature and humidity conditions; S2. The microorganisms to be cultured are transferred into a culture dish, the microorganism culture dish is placed in a culture room, and the adjustable light is adjusted according to the culture requirements of the microorganisms; S3. Close the door and monitor and adjust the temperature and humidity in the culture room through the control panel.

[0016] Beneficial Effects The present invention arranges a humidifying mechanism inside the box body, and through the coordinated arrangement of a water tank, a water injection port, a water delivery pump body, a water delivery pipe, a shunt pipe and an atomizing nozzle, under the conveying action of the water delivery pump body, the water in the water tank can be delivered to the shunt pipe through the water delivery pipe, and then sprayed into the culture chamber by the atomizing nozzle, thereby controlling the humidity in the culture box, making the environment in the culture box more conducive to the growth and reproduction of microorganisms.

[0017] Through the coordinated arrangement of the hot air blower, the circulating fan, the circulating channel, the air inlet, the air outlet and the heat transfer plate, the hot air generated by the hot air blower is heated by the circulating fan and then transported to the heat transfer plate and the surrounding inner walls of the culture chamber through the circulating channel, so that the interior of the culture chamber obtains a uniform and stable temperature, which is more conducive to the growth and reproduction of microorganisms. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the plan structure of a microbial incubator; Figure 2 It is a schematic diagram of the planar structure of the humidification mechanism; Figure 3 for Figure 1 A is an enlarged schematic diagram; Figure 4 for Figure 1 A magnified schematic diagram of B.

[0019] In the attached drawings: 1. box body; 2. box door; 21. transparent window; 3. culture chamber; 31. support frame 1; 32. support frame 2; 33. culture dish placement position; 34. adjustable lighting lamp; 4. humidification mechanism; 41. water tank; 42. water level observation window; 43. water inlet; 44. water pump body; 45. water pipe; 46. shunt pipe 1; 47. shunt pipe 2; 48. atomizing nozzle 1; 49. atomizing nozzle 2; 5. constant temperature mechanism; 51. hot air blower; 52. circulating fan; 53. circulating channel; 54. air inlet; 55. air outlet; 56. heat transfer plate 1; 57. heat transfer plate 2; 6. control console; 61. power switch; 62. control panel. DETAILED DESCRIPTION

[0020] Embodiments of the present invention are described in detail below. The embodiments described below are illustrative and intended to be used to explain the present invention, but should not be construed as limiting the present invention.

[0021] like Figures 1 to 4 As shown, the present invention provides a technical solution A microorganism culture box includes a box body 1, a culture chamber 3, a humidifying mechanism 4 and a constant temperature mechanism 5; the box body 1 is provided with a culture chamber 3; the culture chamber 3 is provided with a support frame 1 31 and a support frame 2 32, and the support frame 1 31 and the support frame 2 32 are provided with a plurality of culture dish placement positions 33; the bottom of the culture chamber 3 is provided with a humidifying mechanism 4, and the working end of the humidifying mechanism 4 extends into the culture chamber 3; the working end of the constant temperature mechanism 5 runs through the culture chamber 3. Through the coordinated arrangement of the box body 1, the culture chamber 3, the humidifying mechanism 4 and the constant temperature mechanism 5, the humidifying mechanism 4 can continuously provide the culture chamber 3 with the specific humidity required for the growth and reproduction of microorganisms, and the constant temperature mechanism 5 runs through the culture chamber 3 to provide the culture chamber 3 with a uniform and stable microorganism culture temperature, thereby ensuring the constant temperature and humidity culture conditions of the microorganisms.

[0022] Preferably, the humidifying mechanism 4 includes a water tank 41, which is fixedly installed at the bottom of the culture chamber 3; a water level observation window 42 is provided in the middle of the front side of the water tank 41, and a water injection port 43 is provided on the upper front side of the water tank 41 so as to be openable and closable. Through the coordinated arrangement of the water tank 41, the water level observation window 42 and the water injection port 43, the water level observation window 42 can be a transparent glass window, and the experimenter can check the water level in the water tank 41 through the water level observation window 42. When the water level is low, the experimenter can complete the water injection by opening the water injection port 43. The operation is simple and convenient, ensuring that the humidifying mechanism 4 can continuously provide a constant humidity environment for the culture chamber 3.

[0023] Preferably, water delivery pump bodies 44 are fixedly installed on both sides of the bottom of the water tank 41; the output ends of the two water delivery pump bodies 44 are respectively connected to water delivery pipes 45; the two water delivery pipes 45 are arranged opposite to each other and extend upward into the culture chamber 3. The two water delivery pipes 45 are arranged upward and extend into the culture chamber 3 to provide the culture chamber 3 with a humidity environment required for microbial culture.

[0024] Preferably, the water pipe 45 extending into the culture chamber 3 is connected to the shunt pipe 1 46 and the shunt pipe 2 47 in sequence; the outlet end of the shunt pipe 1 46 is connected to the atomizing nozzle 1 48, and the outlet end of the shunt pipe 2 47 is connected to the atomizing nozzle 2 49. The shunt pipe 1 46 transports the water in the water tank 41 to the atomizing nozzle 1 48 through the water pipe 45, and the atomizing nozzle 1 48 provides a suitable humidity environment for the culture dish on the support frame 1 31; the shunt pipe 2 47 transports the water in the water tank 41 to the atomizing nozzle 2 49 through the water pipe 45, and the atomizing nozzle 2 49 provides a suitable humidity environment for the culture dish on the support frame 2 32, thereby ensuring that the humidity of the culture chamber 3 is constant.

[0025] Preferably, the constant temperature mechanism 5 includes a hot air blower 51, a circulating air blower 52 and a circulating channel 53; the hot air blower 51 is fixedly installed at the top of the outside of the box 1; the circulating air blower 52 is fixedly installed at the top of the inside of the box 1; the circulating channel 53 runs through the inner wall of the culture chamber 3; the output end of the hot air blower 51 is connected to the circulating channel 53, and the output end of the circulating air blower 52 is connected to the circulating channel 53. The heat generated by the hot air blower 51 enters the inside of the circulating air blower 52, and the circulating air blower 52 performs cyclic heating and then transports the heat to the culture chamber 3 through the circulating channel 53.

[0026] Preferably, two or more air inlets 54 are provided on the inner wall of the culture chamber 3, and an air outlet 55 is provided opposite to the air inlet 54; the air inlet 54 and the air outlet 55 are both provided on the circulation channel 53. The air inlet 54 delivers hot air into the culture chamber 3, and the air inlet 54 and the air outlet 55 are provided opposite to each other, which is conducive to convection of hot air.

[0027] Preferably, a heat transfer plate 1 56 is fixedly connected below the support frame 1 31, and a heat transfer plate 2 57 is fixedly connected below the support frame 2 32; the heat transfer plate 1 56 and the heat transfer plate 2 57 are both connected to the circulation channel 53. Through the coordinated arrangement of the heat transfer plate 1 56 and the heat transfer plate 2 57, the culture dishes on the support frame 1 31 and the support frame 2 32 can obtain a uniform and stable temperature, which is conducive to the constant temperature culture of microorganisms.

[0028] Preferably, an adjustable lighting lamp 34 is provided in the middle of the top of the culture chamber 3. The adjustable lighting lamp 34 can be used to control the lighting variables of microorganism culture.

[0029] Preferably, the outer side of the box body 1 is hinged with a door 2, and a transparent window 21 is provided inside the door 2. The transparent window 21 can observe the growth of microorganisms inside the culture chamber 3 in real time without the need for the experimenter to open the door 2, effectively avoiding the exchange of temperature and humidity conditions inside the culture chamber 3 with the external environment. The bottom of the humidification mechanism 4 is provided with a control console 6, and the control console 6 is provided with a power switch 61 and a control panel 62.

[0030] A working method of a microbial incubator, using the above microbial incubator to culture microorganisms, the specific working method comprises the following steps: S1. Turn on the power switch 61 of the incubator, turn on the hot air blower 51 and the circulating air blower 52, turn on the humidification mechanism 4, and set appropriate culture temperature and humidity conditions; S2, the microorganisms to be cultured are transferred into a culture dish, the microorganism culture dish is placed in the culture chamber 3, and the adjustable light 34 is adjusted according to the culture requirements of the microorganisms; S3, close the door 2, and monitor and adjust the temperature and humidity in the culture chamber 3 through the control panel 62.

[0031] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered in all respects as exemplary and non-restrictive, and the scope of the invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

Claims

1. A microbial incubator, characterized in that: The invention comprises a box body (1), a culture chamber (3), a humidifying mechanism (4) and a constant temperature mechanism (5); the box body (1) is provided with a culture chamber (3); a first support frame (31) and a second support frame (32) are movably provided inside the culture chamber (3); the first support frame (31) and the second support frame (32) are both provided with a plurality of culture dish placement positions (33); a humidifying mechanism (4) is installed at the bottom of the culture chamber (3), and the working end of the humidifying mechanism (4) extends into the culture chamber (3); the working end of the constant temperature mechanism (5) passes through the interior of the culture chamber (3).

2. The microorganism incubator according to claim 1, characterized in that: The humidifying mechanism (4) comprises a water tank (41), and the water tank (41) is fixedly mounted at the bottom end of the culture chamber (3); a water level observation window (42) is provided in the middle of the front side of the water tank (41), and a water injection port (43) is provided on the upper front side of the water tank (41) in an openable and closable manner.

3. The microorganism incubator according to claim 1, characterized in that: Water delivery pump bodies (44) are fixedly mounted on both sides of the bottom end of the water tank (41); the output ends of the two water delivery pump bodies (44) are respectively connected to water delivery pipes (45); the two water delivery pipes (45) are arranged opposite to each other and extend upwards into the culture chamber (3).

4. The microorganism incubator according to claim 3, characterized in that: The water delivery pipe (45) extending into the interior of the culture chamber (3) is connected in sequence to a diversion pipe 1 (46) and a diversion pipe 2 (47); the outlet end of the diversion pipe 1 (46) is connected to an atomizing nozzle 1 (48), and the outlet end of the diversion pipe 2 (47) is connected to an atomizing nozzle 2 (49).

5. The microorganism incubator according to claim 1, characterized in that: The constant temperature mechanism (5) comprises a hot air blower (51), a circulating air blower (52) and a circulating channel (53); the hot air blower (51) is fixedly mounted on the top of the outside of the box (1); the circulating air blower (52) is fixedly mounted on the top of the inside of the box (1); the circulating channel (53) runs through the inner wall of the culture chamber (3); the output end of the hot air blower (51) is connected to the circulating channel (53), and the output end of the circulating air blower (52) is connected to the circulating channel (53).

6. The microorganism incubator according to claim 5, characterized in that: Two or more air inlets (54) are provided on the inner wall of the culture chamber (3), and an air outlet (55) is provided opposite to the air inlet (54); the air inlet (54) and the air outlet (55) are both provided on the circulation channel (53).

7. The microorganism incubator according to claim 5, characterized in that: A heat transfer plate 1 (56) is fixedly connected below the support frame 1 (31), and a heat transfer plate 2 (57) is fixedly connected below the support frame 2 (32); the heat transfer plate 1 (56) and the heat transfer plate 2 (57) are both connected to the circulation channel (53).

8. The microorganism incubator according to claim 1, characterized in that: An adjustable lighting lamp (34) is arranged in the middle of the top of the culture chamber (3).

9. The microorganism incubator according to claim 1, characterized in that: The outer side surface of the box body (1) is hinged with a box door (2), and a transparent window (21) is arranged inside the box door (2).

10. A working method of a microorganism incubator according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1. Turn on the power switch (61) of the incubator, turn on the hot air blower (51) and the circulating air blower (52), turn on the humidification mechanism (4), and set appropriate incubation temperature and humidity conditions; S2, transferring the microorganisms to be cultured into a culture dish, placing the microorganism culture dish in a culture chamber (3), and adjusting the adjustable light (34) according to the culture requirements of the microorganisms; S3, closing the box door (2), and monitoring and regulating the temperature and humidity in the culture chamber (3) through the control panel (62).

Images