Long-time-sequence microorganism enrichment sampling cup and application method thereof

Abstract

The invention discloses a long-time-sequence microorganism enrichment sampling cup which comprises a cup body and a cup cover meshed with the cup body in a sealed mode, a multi-stage filtering assembly is arranged in the cup body, a liquid injection opening, a multi-stage liquid injection pipeline, a multi-stage liquid outlet pipeline and a pressure relief pipeline are arranged on the cup body and the cup cover, and a plug is arranged at an end opening of the liquid outlet pipeline. The device has the beneficial effects that through a three-stage membrane filtering structure, impurities are effectively removed, and the effective concentration of a sampled product is improved; a plurality of liquid injection ports are arranged, after a sample is filtered, multi-stage injection of the stationary liquid is realized, the stationary liquid is ensured to be in full contact with the sample, the impurity content is reduced, and the microorganism purity and the sampling efficiency are improved; a plurality of pressure release valves are arranged, so that after the sample enrichment operation is completed, the pressure of the sampling cup is quickly released, the sampling process is simplified, and the scientific investigation experiment efficiency is improved; the device adopts a modular design, is simple and stable in structure, is convenient to operate and sample, and is particularly suitable for carrying and transporting scenes of underwater vehicles in scientific investigation experiments.

Description

Technical Field

[0001] The invention belongs to the technical field of bioengineering, and specifically relates to a long-time-sequence microorganism enrichment sampling cup and an application method thereof. Background Art

[0002] With the continuous deepening of marine scientific research, the collection and enrichment of microbial samples have important research value in the fields of marine biology, environmental science and bioengineering. Marine microorganisms not only play a key role in ecological balance, but also show broad application prospects in the fields of new drug development, enzyme engineering and environmental restoration. Therefore, accurate and effective collection of microbial samples, especially long-term enrichment, is crucial to improving the quality of scientific research results.

[0003] At present, the existing microbial sampling devices usually use filtration and concentration to enrich microbial samples. However, the existing technology has the following main problems:

[0004] 1. Low filtration efficiency and poor impurity removal effect: Traditional sampling devices mostly use a single membrane filtration system, which is easy to be mixed with impurities during the filtration process, resulting in low sample concentration and insufficient purity, affecting the accuracy of subsequent experiments.

[0005] 2. Uneven injection of fixative solution, low purity of microorganisms: Existing devices often fail to achieve sufficient contact when injecting fixative solution, resulting in unsatisfactory fixation effect and high impurity content, which affects the purity of microorganisms and sample quality.

[0006] 3. The pressure relief operation is cumbersome, affecting the sampling efficiency: the existing sampling equipment lacks an effective pressure relief design after completing filtration and enrichment, which makes the sampling process cumbersome and time-consuming, reducing the efficiency of scientific research experiments.

[0007] 4. Complex structure and poor portability: Some sampling devices have complex structural designs, poor stability, and are difficult to adapt to the requirements of underwater vehicles in marine scientific research, which brings inconvenience to actual use. Summary of the invention

[0008] The main purpose of the present invention is to provide a long-time microorganism enrichment sampling cup with good impurity removal effect and high effective sample concentration and an application method thereof.

[0009] The long-time microbial enrichment sampling cup provided by the present invention comprises a cup body and a cup cover which is meshed and sealed with the cup body. A multi-stage filtering assembly is arranged in the cup body. A liquid injection port, a multi-stage liquid injection pipe, a multi-stage liquid outlet pipe and a pressure relief pipe are arranged on the cup body and the cup cover. A plug is arranged at the port of the liquid outlet pipe.

[0010] In one embodiment of the above-mentioned sampling cup, the cup body is a cylindrical hollow cup, on which a circular cup cover connected by threads is arranged.

[0011] In one embodiment of the above-mentioned sampling cup, a downward connecting port is provided at the bottom end of the inner cavity of the cup body, wherein a one-way valve for injecting only liquid is installed.

[0012] In one embodiment of the above-mentioned sampling cup, the top of the inner cavity of the cup cover is provided with an upward connecting port, in which a one-way valve for only injecting liquid is installed.

[0013] In one embodiment of the sampling cup, three groups of filter components are equidistantly arranged from top to bottom in the inner cavity of the cup body, and each group of filter components includes a filter sleeve, a gasket and a liner.

[0014] In one embodiment of the above-mentioned sampling cup, a plurality of filter bushings are arranged around the side wall of the cup body at the same intervals, and O-shaped gaskets are provided at the upper and lower ends of the filter bushings; the lining plate is provided below the filter bushing, and the lining plate is clamped by the gasket at the lower end of the filter bushing and the gasket at the upper end of the lower group of filter bushings.

[0015] In one embodiment of the above-mentioned sampling cup, the liquid injection pipe, the liquid outlet pipe and the pressure relief pipe are arranged and connected on the outer wall of the cup body; the liquid outlet pipe and the pressure relief pipe are located on the same side, and the liquid injection pipe is located on the opposite side.

[0016] In one embodiment of the above-mentioned sampling cup, the liquid injection pipe and the liquid outlet pipe are each provided at three locations from top to bottom, and each location is correspondingly provided above the filter membrane of each level of the filter assembly.

[0017] A method for using the above-mentioned long-time microbial enrichment sampling cup, the specific steps are as follows:

[0018] 1. Seawater or other microbial sample liquid is pumped into the outer shell through the top of the sampling cup and enters the multi-stage filtration assembly;

[0019] 2. The liquid passes through multiple groups of filter components in sequence;

[0020] 3. Through the multi-stage liquid injection pipeline arranged on the cup body, the fixing liquid is injected into the cup body in multiple stages to perform filtering and enrichment operations;

[0021] 4. After the filtration and enrichment operation is completed, remove the plugs of the outlet pipe ports one by one, and release the pressure through the pressure relief pipe set on the cup body;

[0022] 5. After the pressure is released, remove the cup cover and start taking out the sample.

[0023] The beneficial effects of the present invention are:

[0024] 1. Through the three-stage membrane filtration structure, impurities can be effectively removed and the effective concentration of the sample can be increased;

[0025] 2. Multiple injection ports are configured to achieve multi-stage injection of the fixative after sample filtration is completed, ensuring full contact between the fixative and the sample, reducing impurity content, and improving microbial purity and sampling efficiency;

[0026] 3. Set up multiple pressure relief valves to quickly release the pressure in the sampling cup after the sample enrichment operation is completed, simplify the sampling process, and improve the efficiency of scientific research experiments;

[0027] 4. It adopts modular design, with simple and stable structure, easy to operate and sample, and is particularly suitable for the transportation scenarios of underwater vehicles in scientific research experiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a schematic diagram of the axonometric structure of an embodiment of the present invention.

[0029] Figure 2 for Figure 1 Schematic diagram of the cross-sectional structure. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the relevant technical solutions. Obviously, the described embodiments are only part of the embodiments, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] like Figure 1 and Figure 2 As shown, the long-time microorganism enrichment sampling cup disclosed in this embodiment includes a cup body 1, a cup cover 2 and a filter assembly 3.

[0032] The cup body 1 is a cylindrical hollow cup, on which a threaded circular cup cover 2 is arranged. The cup cover can be taken out after the filtration and enrichment work is completed, which is convenient for the subsequent removal and operation of the sample.

[0033] The bottom of the inner cavity of the cup body 1 is provided with a downward connection port, in which a one-way valve 11 is installed, which can only inject liquid; the top of the inner cavity of the cup cover 2 is provided with an upward connection port, in which a one-way valve 21 is installed, which can only inject liquid. The one-way valve controls the one-way flow of liquid to ensure the smooth filtration process and avoid backflow.

[0034] Three groups of filter components 3 are equidistantly arranged from top to bottom in the inner cavity of the cup body 1 , and each group of filter components includes a filter bushing 31 , a gasket 32 ​​and a lining plate 33 .

[0035] A plurality of filter bushings 31 are arranged around the side wall of the cup body 1 at the same intervals, and an O-shaped gasket 32 ​​is arranged at the upper and lower ends of the filter bushings. A lining plate 33 is arranged below the filter bushing, and the lining plate is clamped by the gasket at the lower end of the filter bushing and the gasket at the upper end of the lower group of filter bushings.

[0036] The three groups of filter components form a three-stage filtration system, which removes impurities in the liquid step by step, effectively improving the purity and effective concentration of the sample.

[0037] A liquid injection pipe 12 , a liquid discharge pipe 13 and a pressure relief pipe 14 are arranged on the outer wall of the cup body 1 and are connected thereto.

[0038] The liquid outlet pipe 13 and the pressure relief pipe 14 are located on the same side, and the liquid injection pipe 12 is located on the opposite side.

[0039] The injection pipe 12 and the outlet pipe 13 are both provided at three locations from top to bottom, and are correspondingly provided above the three groups of filter membranes of the filter assembly 3. The multi-stage injection port design ensures that the stationary liquid is fully in contact with the sample, reduces the impurity content, and further improves the purity of the microbial sample.

[0040] A manually removable plug is placed at the end of the liquid outlet pipe 13.

[0041] The application method of this sampling cup is as follows:

[0042] 1. Seawater or other microbial sample liquid is pumped into the outer shell through the top of the sampling cup and enters the three-stage filtration system;

[0043] 2. The liquid passes through three groups of filter components in the three-stage filtration system in sequence;

[0044] 3. Then, the fixed liquid is injected into the filtration system in multiple stages through the injection pipes arranged at different positions of the outer shell to perform filtration and enrichment operations;

[0045] 4. After the filtration and enrichment operation is completed, remove the plugs of the outlet pipe ports in turn and release the pressure through the pressure relief valve set on the outer shell;

[0046] 5. After the pressure is released, take out the cup cover and remove the sample to facilitate subsequent experimental analysis.

[0047] This long-term microbial enrichment sampling cup has the following characteristics:

[0048] 1. Through the three-stage membrane filtration design, impurities are removed step by step to improve the effective concentration and purity of the sample.

[0049] 2. Multiple injection ports ensure full contact between the fixative and the sample, achieving efficient fixation and improving sample quality.

[0050] 3. The pressure relief valve design simplifies the pressure relief operation, improves sampling efficiency, and is suitable for rapid operation in field scientific research experiments.

[0051] 4. The modular design makes the overall structure simple and stable, easy to operate, and particularly suitable for carrying underwater vehicles for scientific research experiments.

[0052] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although detailed descriptions have been made with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein with equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A long-time microbial enrichment sampling cup, characterized in that: The invention comprises a cup body and a cup cover which is meshed and sealed with the cup body. A multi-stage filtering assembly is arranged in the cup body. A liquid injection port, a multi-stage liquid injection pipeline, a multi-stage liquid outlet pipeline and a pressure relief pipeline are arranged on the cup body and the cup cover. A plug is arranged at the port of the liquid outlet pipeline.

2. The long-time microbial enrichment sampling cup according to claim 1, characterized in that: The cup body is a cylindrical hollow cup, on which a circular cup cover connected by threads is arranged.

3. The long-time microbial enrichment sampling cup according to claim 1, characterized in that: The bottom end of the inner cavity of the cup body is provided with a downward connecting port, in which a one-way valve for only injecting liquid is installed.

4. The long-time microbial enrichment sampling cup according to claim 1, characterized in that: The top of the inner cavity of the cup cover is provided with an upward connecting port, in which a one-way valve for only injecting liquid is installed.

5. The long-time microbial enrichment sampling cup according to claim 1, characterized in that: The filter components are arranged in three groups equidistantly from top to bottom in the inner cavity of the cup body, and each group of the filter components includes a filter sleeve, a gasket and a lining plate.

6. The long-time microorganism enrichment sampling cup according to claim 5, characterized in that: A plurality of filter bushings are arranged around the side wall of the cup body at the same intervals, and O-shaped gaskets are arranged at the upper and lower ends of the filter bushings; the lining plate is arranged below the filter bushing, and the lining plate is clamped by the gasket at the lower end of the filter bushing and the gasket at the upper end of the lower group of filter bushings.

7. The long-time microbial enrichment sampling cup according to claim 1, characterized in that: The liquid injection pipe, the liquid outlet pipe and the pressure relief pipe are arranged on the outer wall of the cup body and are connected to each other; the liquid outlet pipe and the pressure relief pipe are located on the same side, and the liquid injection pipe is located on the opposite side.

8. The long-time microorganism enrichment sampling cup according to claim 5, characterized in that: The liquid injection pipeline and the liquid outlet pipeline are both provided at three locations from top to bottom, and each location is correspondingly arranged above the filter membrane of each level of filter assembly.

9. A method for using the long-time microbial enrichment sampling cup according to claims 1-8, the specific steps being as follows:

1. Seawater or other microbial sample liquid is pumped into the outer shell through the top of the sampling cup and enters the multi-stage filtration assembly; 2. The liquid passes through multiple groups of filter components in sequence; 3. Through the multi-stage liquid injection pipeline arranged on the cup body, the fixing liquid is injected into the cup body in multiple stages to perform filtering and enrichment operations; 4. After the filtration and enrichment operation is completed, remove the plugs of the outlet pipe ports one by one, and release the pressure through the pressure relief pipe set on the cup body; 5. After the pressure is released, remove the cup cover and start taking out the sample.

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