Cell slide preparation equipment

Abstract

The cell slide preparation equipment comprises a support frame, a suction head, M capillary tube groups, a moving assembly, a suction tube table, a separation frame, a recovery box, a test tube rack, a slide clamp, a sedimentation bin, a reagent bottle rack, a sample introduction piece, a double-tube clamping valve, a single-tube clamping valve, a vacuum pump, a waste liquid tank, a control panel and a display screen, the sucker, the capillary group and the moving component are mounted on the upper layer of the support frame; the straw table, the separation rack, the recovery box, the test tube rack, the slide clamp and the reagent bottle rack are arranged on the lower layer of the support frame; the N groups of sample introduction pieces, the N + 1 double-tube pinch valves, the M * N single-tube pinch valves, the vacuum pump, the waste liquid tank and the control panel are arranged on the back surface of the support frame; the vacuum pump is connected with the waste liquid tank through a hose; the waste liquid tank is connected with the liquid suction capillaries in all the capillary groups through hoses; the display screen is arranged on the top cover of the supporting frame and connected with the control panel, through mutual cooperation of all the components, the whole cell slide preparation and dyeing process of a plurality of slides can be automatically completed at the same time, and the cell slide preparation efficiency is effectively improved.

Description

Technical Field

[0001] The present application relates to the field of automatic control technology, and in particular to a cell preparation device. Background Art

[0002] Cytomorphological examination is one of the diagnostic methods in pathology and is often used to diagnose a variety of diseases, including leukemia, multiple myeloma, lymphoma, anemia, and cervical cancer. Cytomorphological examination requires the preparation and staining of cell tissues and then examination under a microscope.

[0003] In the prior art, the cell preparation process is as follows: extract cell tissue to prepare a cell solution, mix the cell solution with a drug reagent, use a centrifuge to centrifuge the cell solution mixed with the drug reagent, discard the supernatant and transfer it to an injection device for dilution, then pour out the upper liquid and transfer it to a staining device for staining. The entire process requires a lot of manual intervention and the participation of multiple devices, resulting in low efficiency in cell preparation.

[0004] With regard to the problem of low efficiency of cell preparation in the prior art, no effective solution has been proposed yet. Summary of the invention

[0005] Based on this, it is necessary to provide a cell preparation device to address the above technical issues and solve the problem of low efficiency of cell preparation in related technologies.

[0006] The embodiment of the present application provides a cell preparation device, including a support frame, a pipette tip, M capillary groups, a moving component, a pipette table, a detachment frame, a recovery box, a test tube rack, a slide clamp, a sedimentation chamber, a reagent bottle rack, a sample injection part, a double-tube clamp valve, a single-tube clamp valve, a vacuum pump, a waste liquid tank, a control panel and a display screen; the pipette tip, the capillary group and the moving component are installed on the upper layer of the support frame, the moving component can drive the pipette tip to move along the X direction, the Y direction and the Z direction, the moving component can drive the capillary group to move along the X direction and the Z direction, and each capillary group includes 1 liquid suction capillary and N liquid discharge capillaries; wherein N is the total number of buffer and dye types, M≥1, N≥1;

[0007] The pipette table, the detachment rack, the recovery box, the test tube rack, the glass slide clamp and the reagent bottle rack are arranged in the lower layer of the support frame, and the pipette head can be inserted into the pipette on the pipette table by interference fit; the detachment rack can peel the pipette from the pipette head and peel it into the recovery box; the glass slide clamp is provided with M glass slide grooves at the same X-axis coordinate, and the glass slide grooves correspond to the Y-axis coordinates of the capillary group one by one; the sedimentation chamber is a hollow cylindrical shape, and the sedimentation chamber can press the glass slide and fix the glass slide on the glass slide groove;

[0008] N groups of the sample injection parts, N+1 double-tube pinch valves, M×N single-tube pinch valves, the vacuum pump, the waste liquid tank and the control panel are arranged on the back of the support frame; each group of the sample injection parts includes a sample injection motor and a sampler, and the sample injection motor can drive the sampler to perform piston movement; one of the samplers is connected to a hose, and the hose 1 is divided into 2 and passes through the double-tube pinch valve, and 1 hose is connected to the buffer solution placed on the reagent bottle rack, and the other hose is divided into 2 and passes through the double-tube pinch valve, and 1 hose is connected to the suction head, and the other hose is divided into M, and each hose passes through different single-tube pinch valves and is connected to each of the hairs One of the drainage capillaries in the capillary group is connected; the pipeline connection method of the remaining N-1 samplers is as follows: the sampler is connected with a hose, the hose 1 is divided into 2 and passes through a double-tube clamp valve, one hose is connected to the dye placed on the reagent bottle rack, and the other hose 1 is divided into M, each hose passes through a different single-tube clamp valve and is connected to one of the drainage capillaries in each capillary group; the vacuum pump is connected to the waste liquid tank through a hose, and the waste liquid tank is connected to the suction capillaries in all capillary groups through a hose; the display screen is arranged on the top cover of the support frame, and the display screen is connected to the control panel.

[0009] In one embodiment, the moving assembly includes an X-axis translation assembly, a Y-axis translation assembly, a Z1-axis translation assembly and a Z2-axis translation assembly; the X-axis translation assembly includes an X-axis linear guide, an X-axis slider and an X-motor, the X-axis linear guide is installed on the upper layer of the support frame, and the X-motor can drive the X-axis slider to slide in the X-axis linear guide; the Y-axis translation assembly includes a Y-axis mounting plate, a Y-axis linear guide, a Y-axis slider and a Y-motor, the Y-axis mounting plate is installed on the X-axis slider, the Y-axis linear guide is installed on the Y-axis mounting plate, and the Y-motor can drive the Y-axis slider to slide in the Y-axis linear guide; the Z1-axis translation assembly includes a Z1 The Z2-axis translation assembly comprises a Z2-axis housing, a Z2-axis linear guide, a Z2-axis slider and a Z2 motor, wherein the Z1-axis housing is connected to the Y-axis slider, the Z1-axis linear guide is installed in the Z1-axis housing, the Z1 motor can drive the Z1-axis slider to slide in the Z1-axis linear guide, and the suction head is connected to the Z1-axis slider; the Z2-axis translation assembly comprises a Z2-axis housing, a Z2-axis linear guide, a Z2-axis slider and a Z2 motor, the Z2-axis housing is connected to the Y-axis mounting plate, the Z2-axis linear guide is installed in the Z2-axis housing, the Z2 motor can drive the Z2-axis slider to slide in the Z2-axis linear guide, and M capillary groups are connected to the Z2-axis slider.

[0010] In one embodiment, the cell preparation device further comprises four adjustable foot cups, and the four adjustable foot cups are installed at the bottom of the cell preparation device.

[0011] In one embodiment, the cell preparation device further includes a waste liquid tank, which is disposed at the lower layer of the support frame and is used to recycle waste liquid.

[0012] The cell preparation device comprises a support frame, a pipette tip, M capillary groups, a moving assembly, a pipette table, a detachment frame, a recovery box, a test tube rack, a glass slide clamp, a sedimentation chamber, a reagent bottle rack, a sample injection part, a double-tube clamp valve, a single-tube clamp valve, a vacuum pump, a waste liquid tank, a control panel and a display screen; the pipette tip, the capillary group and the moving assembly are installed on the upper layer of the support frame, the moving assembly can drive the pipette tip to move in the X direction, the Y direction and the Z direction, the moving assembly can drive the capillary group to move in the X direction and the Z direction, and each capillary group comprises 1 liquid suction capillary and N liquid discharge capillaries; wherein N is the number of buffer and dye species. The total number of types, M≥1, N≥1; the pipette table, the detachment rack, the recovery box, the test tube rack, the slide clamp and the reagent bottle rack are arranged in the lower layer of the support frame, and the pipette tip can be inserted into the pipette on the pipette table by interference fit; the detachment rack can peel the pipette from the pipette tip and peel it into the recovery box; the slide clamp is provided with M slide slots at the same X-axis coordinate, and the slide slots correspond to the Y-axis coordinates of the capillary group one by one; the sedimentation chamber is a hollow cylinder, and the sedimentation chamber can press the slide and fix the slide on the slide slot; N groups of sample injection parts, N+1 double-tube pinch valves, M×N single-tube pinch valves, vacuum pumps, waste liquid tanks and control panels Arranged on the back of the support frame; each group of sample injection parts includes a sample injection motor and a sampler, and the sample injection motor can drive the sampler to perform piston movement; one of the samplers is connected to a hose, and the hose 1 is divided into 2 and passes through a double-tube clamp valve, and 1 hose is connected to the buffer solution placed on the reagent bottle rack, and the other hose is divided into 2 and passes through a double-tube clamp valve, and 1 hose is connected to the suction head, and the other hose is divided into M, and each hose passes through a different single-tube clamp valve and is connected to one of the discharge capillaries in each capillary group; the pipeline connection method of the remaining N-1 samplers is as follows: the sampler is connected to a hose, The hose 1 is divided into 2 and passes through a double-tube clamp valve, and one hose is connected to a dye placed on a reagent bottle rack, and the other hose 1 is divided into M. Each hose passes through a different single-tube clamp valve and is connected to one of the discharge capillaries in each capillary group; the vacuum pump is connected to a waste liquid tank through a hose, and the waste liquid tank is connected to the suction capillaries in all capillary groups through a hose; a display screen is arranged on a top cover of a support frame, and the display screen is connected to a control panel. Through the mutual cooperation of various components, the present application can automatically complete the entire process of cell preparation and staining of multiple slides at the same time, thereby effectively improving the efficiency of cell preparation. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0014] Figure 1 is a front schematic diagram of a cell preparation device provided according to an embodiment of the present application;

[0015] Figure 2 is a schematic diagram of the back side of a cell preparation device provided in an embodiment of the present application;

[0016] Figure 3 It is a schematic diagram of a pipette tip and surrounding components in a cell preparation device provided in an embodiment of the present application;

[0017] Figure 4 It is a schematic diagram of a capillary group and its surrounding components in a cell preparation device provided in an embodiment of the present application;

[0018] Figure 5 A schematic diagram of a sample injection device in a cell preparation device provided in the present application;

[0019] Figure 6 Schematic diagram of the pipe connection method in the cell preparation device provided in this application Figure 1 ;

[0020] Figure 7 Schematic diagram of the pipe connection method in the cell preparation device provided in this application Figure 2 . DETAILED DESCRIPTION

[0021] In order to make the purpose, technical solutions and advantages of the present application clearer, the present application is described and illustrated below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and are not intended to limit the present application. Based on the embodiments provided in the present application, all other embodiments obtained by ordinary technicians in the field without making creative work are within the scope of protection of the present application.

[0022] Obviously, the drawings described below are only some examples or embodiments of the present application. For ordinary technicians in this field, the present application can also be applied to other similar scenarios based on these drawings without creative work. In addition, it can also be understood that although the efforts made in this development process may be complicated and lengthy, for ordinary technicians in this field related to the content disclosed in this application, some changes in design, manufacturing or production based on the technical content disclosed in this application are just conventional technical means, and should not be understood as insufficient content disclosed in this application.

[0023] Reference to "embodiments" in this application means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those of ordinary skill in the art that the embodiments described in this application may be combined with other embodiments without conflict.

[0024] Unless otherwise defined, the technical terms or scientific terms involved in this application should be understood by people with ordinary skills in the technical field to which this application belongs. The words "one", "a", "a", "the" and the like involved in this application do not indicate a quantitative limitation and may represent the singular or plural. The terms "include", "comprise", "have" and any of their variations involved in this application are intended to cover non-exclusive inclusions; for example, a process, method, system, product or device that includes a series of steps or modules (units) is not limited to the listed steps or units, but may also include unlisted steps or units, or may also include other steps or units inherent to these processes, methods, products or devices. The words "connect", "connected", "coupled" and the like involved in this application are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. The "multiple" involved in this application refers to two or more. "And / or" describes the association relationship of associated objects, indicating that there may be three relationships, for example, "A and / or B" can mean: A exists alone, A and B exist at the same time, and B exists alone. The character " / " generally indicates that the objects before and after are in an "or" relationship. The terms "first", "second", "third", etc. involved in this application are only used to distinguish similar objects and do not represent a specific ordering of the objects.

[0025] Figure 1 This is a front view of the cell preparation device provided in this application. Figure 2 This is a schematic diagram of the back of the cell preparation device provided in this application. Figure 3 This is a schematic diagram of the pipette tip and surrounding components in the cell preparation device provided in this application. Figure 4 A schematic diagram of a capillary group and its surrounding components in a cell preparation device provided in this application, Figure 5 A schematic diagram of a sample injection device in a cell preparation device provided in this application, Figure 6 Schematic diagram of the pipe connection method in the cell preparation device provided in this application Figure 1 , Figure 7 Schematic diagram of the pipe connection method in the cell preparation device provided in this application Figure 2 ,like Figures 1 to 7As shown, the cell preparation device 1000 provided in the present application includes a support frame 1, a pipette tip 2, M capillary groups 3, a moving component, a pipette table 4, a detachment frame 5, a recovery box 6, a test tube rack 7, a slide clamp 8, a sedimentation chamber 9, a reagent bottle rack 10, a sample injection part 11, a double-tube clamp valve 12, a single-tube clamp valve 13, a vacuum pump 14, a waste liquid tank 15, a control panel 16 and a display screen 17; the pipette tip 2, the capillary group 3 and the moving component are installed on the upper layer of the support frame 1, and the moving component can drive the pipette tip 2 to move in the X direction, the Y direction and the Z direction; the moving component can drive the capillary group 3 to move in the X direction and the Z direction, and each capillary group 3 includes 1 liquid suction capillary and N capillaries. Capillary discharge tube; wherein N is the total number of buffer and dye types, M≥1, N≥1; pipette table 4, detachment rack 5, recovery box 6, test tube rack 7, slide clamp 8 and reagent bottle rack 10 are arranged on the lower layer of the support frame 1, and the pipette tip 2 can be inserted into the pipette placed on the pipette table 4 by interference fit; the detachment rack 5 can peel the pipette from the pipette tip 2 and peel it into the recovery box 6; the slide clamp 8 sets M slide grooves at the same X-axis coordinate, and the slide grooves correspond to the Y-axis coordinates of the capillary group 3 one by one; the sedimentation chamber 9 is a hollow cylindrical shape, and the sedimentation chamber 9 can press the slide and fix the slide on the slide groove; N groups of sample injection parts 11, N+1 double-tube clamp valves 12, M×N single-tube pinch valves 13, vacuum pump 14, waste liquid tank 15 and control panel 16 are arranged on the back of support frame 1; each group of sample injection parts 11 includes a sample injection motor 1101 and a sampler 1102, and the sample injection motor 1101 can drive the sampler 1102 to do piston movement; one of the samplers 1102 is connected to a hose, and the hose 1 is divided into 2 and passes through the double-tube pinch valve 12, and one hose is connected to the buffer solution placed on the reagent bottle rack 10, and the other hose is divided into 2 and passes through the double-tube pinch valve 12, and one hose is connected to the suction head 2, and the other hose is divided into M, and each hose passes through different single-tube pinch valves 13 and is connected to each capillary group 3. The pipe connection method of the remaining N-1 samplers 1102 is as follows: the sampler 1102 is connected to a hose, the hose 1 is divided into 2 and passes through a double-tube clamp valve 12, one hose is connected to the dye placed on the reagent bottle rack 10, and the other hose 1 is divided into M, each hose passes through a different single-tube clamp valve 13 and is connected to one of the drainage capillaries in each capillary group 3; the vacuum pump 14 is connected to the waste liquid tank 15 through a hose, and the waste liquid tank 15 is connected to the suction capillaries in all capillary groups 3 through a hose; the display screen 17 is arranged on the top cover of the support frame 1, and the display screen 17 is connected to the control board 16.

[0026] Specifically, the preparatory work for using the cell slide making device 1000 includes: extracting cell tissue to prepare cell solution, transferring the cell solution to a test tube placed on the test tube rack 7, placing a slide on the slide slot of the slide holder 8, pressing the sedimentation chamber 9 on the slide and fixing the slide on the slide slot. After the preparatory work, the user can issue a slide making instruction through the operating display screen. After receiving the instruction, refer to Figure 6 , the control board 16 controls the ab passage of the double-tube pinch valve 12 to open, the cd passage to close, and controls the injection motor 1101 on the injection part 11 to open, and the injection motor 1101 drives the sampler 1102 to do piston movement to absorb the buffer. After the sampler 1102 absorbs the buffer, the control board 16 controls the moving component to drive the suction head 2 to do a compound movement along the X direction, the Y direction and the Z direction. When the suction head 2 reaches the top of the test tube placed on the test tube rack 7, the control board 16 controls the ab passage of the double-tube pinch valve 12 to close, the cd passage to open, and the ef passage to open, and the gh passage to close. The control board 16 controls the injection motor 1101 to drive the sampler 1102 to do piston movement to release the buffer, and the buffer is released into the test tube through the suction head 2. The control board 16 then controls the moving assembly to drive the suction head 2 to perform compound motion along the X, Y and Z directions, so that the suction head 2 is inserted into the suction tube placed on the suction tube table 4 with interference fit, and then controls the moving assembly to drive the suction head 2 to perform compound motion. Since the suction head 2 is inserted into the suction tube, the suction tube moves with the suction head 2. Through this compound motion, the suction tube is inserted into the test tube, and the control board 16 controls the injection motor 1101 to drive the sampler 1102 to perform piston motion to suck the cell solution into the suction tube. After that, the suction tube is moved to the sedimentation bin position through the compound motion, and the injection motor 1101 is controlled to drive the sampler 1102 to perform piston motion to discharge the cell solution from the suction tube into the sedimentation bin 9. After the cell solution is discharged into the sedimentation bin 9, the suction tube is moved to the bottom of the separation rack 5 through the compound motion, and the moving assembly is controlled to drive the suction head 2 to move up, and the separation rack 5 peels the suction tube from the suction head 2, and the used suction tube returns to the recovery box 6, and the recovery box 6 can be taken out from the support frame 1 for easy cleaning. As one of the implementation methods, the slide holder in the present application can be provided with no less than two slide slots at the same X-axis coordinate, and the slide slots can also be numbered, and then the slide with the corresponding serial number can be selected on the display screen for slide preparation. In addition, it is also possible to select to set the slide slots at multiple different X-axis coordinates, so that the cell slide preparation device 1000 in the present application has more slide slots available, so as to Figure 1 For example, the slide holder in the embodiment of the present invention has 4 slide slots at the same X-axis coordinate, and the slide slots are set at 12 different X-axis coordinates. Then, the cell preparation device 1000 in the present application has 48 slide slots available. In addition, each position for placing a test tube on the test tube rack 7 can also be numbered accordingly, and the number corresponds to the number of the slide slot one by one to avoid confusion of different cell solutions.

[0027] In addition, if multiple slides need to be prepared, the above step of adding the cell solution is repeated until the cell solution is discharged into the sedimentation bins corresponding to all the slides that need to be prepared.

[0028] Furthermore, the control board 16 controls the moving assembly to drive the capillary group 3 to perform a compound movement in the X direction and the Z direction, and moves the capillary group 3 to the top of the sedimentation chamber 9. Since the Y coordinates of the glass slide groove and the capillary group 3 correspond one to one, the glass slide groove and the capillary group 3 correspond one to one at this time. Figure 7 , the control board 16 controls the ij passage of the double-tube pinch valve 12 to open and the kp passage to close, and the control board 16 controls the injection motor 1101 to drive the sampler 1102 to do piston movement to absorb the dye. When the sampler 1102 completes the operation of absorbing the dye, the ij passage of the double-tube pinch valve 12 is controlled to close and the kp passage is opened, and according to which slides need to add the current type of dye under this X-axis coordinate, the control board 16 controls the corresponding single-tube pinch valve 13 to open, and then controls the injection motor 1101 to drive the sampler 1102 to do piston movement to release the dye, and the dye is discharged from the drainage capillary into the sedimentation chamber 9 of the corresponding slide. Taking the case where the current type of stain needs to be added to glass slide No. 1 and glass slide No. 3 as an example, the control panel 16 controls the single-tube pinch valve No. (1) and the single-tube pinch valve No. (3) to open, and then controls the injection motor 1101 to drive the sampler 1102 to perform piston movement to release the stain, and the stain is discharged from the drainage capillary of the first capillary group into the sedimentation chamber 9 of glass slide No. 1 and from the drainage capillary of the third capillary group into the sedimentation chamber 9 of glass slide No. 3.

[0029] Of course, if you need to add buffer to the sedimentation chamber during this period, see Figure 6 The control board 16 can control the ab passage of the double-tube pinch valve 12 to open and the cd passage to close, and control the injection motor 1101 on the injection part 11 to start, and the injection motor 1101 drives the sampler 1102 to do piston movement to absorb the buffer. After the sampler 1102 absorbs the buffer, the control board 16 controls the ab passage of the double-tube pinch valve 12 to close, the cd passage to open, and the ef passage to close and the gh passage to open, and according to which glass slides need to add buffer under this X-axis coordinate, the control board 16 controls the corresponding single-tube pinch valve 13 to open, and the control board 16 controls the injection motor 1101 to drive the sampler 1102 to do piston movement to release the buffer, and the buffer is discharged from the discharge capillary to the sedimentation chamber 9 of the corresponding glass slide.

[0030] After the cells in the cell solution naturally settle and adhere to the glass slide in the sedimentation chamber 9, the control panel 16 is turned on to control the vacuum pump 14 to start. The vacuum pump 14 adds negative pressure to the waste liquid tank 15, and the liquid suction capillary sucks the excess buffer and stain in the sedimentation chamber 9 into the waste liquid tank 15.

[0031] If there are other slides in the X-axis coordinate that need to be added with dye and buffer, the control board 16 controls the moving assembly to drive the capillary group 3 to continue moving along the X-axis, repeating the above-mentioned dye adding operation, buffer adding operation and waste liquid suction operation to complete the cell slide preparation process.

[0032] In the prior art, the cell preparation process is as follows: extract cell tissue to prepare a cell solution, mix the cell solution with a drug reagent, use a centrifuge to centrifuge the cell solution mixed with the drug reagent, discard the supernatant and transfer it to an injection device for dilution, then pour out the upper liquid and transfer it to a staining device for staining. The entire process requires a lot of manual intervention and the participation of multiple devices, resulting in low efficiency in cell preparation.

[0033] In order to solve the above problems, the present application provides a cell preparation device 1000, including a support frame 1, a pipette tip 2, M capillary groups 3, a moving component, a pipette table 4, a detachment frame 5, a recovery box 6, a test tube rack 7, a slide clamp 8, a sedimentation chamber 9, a reagent bottle rack 10, a sample injection part 11, a double-tube clamp valve 12, a single-tube clamp valve 13, a vacuum pump 14, a waste liquid tank 15, a control panel 16 and a display screen 17; the pipette tip 2, the capillary group 3 and the moving component are installed on the upper layer of the support frame 1, and the moving component can drive the pipette tip 2 to move along the X direction, the Y direction and the Z direction; the moving component can drive the capillary group 3 to move along the X direction and the Z direction, and each capillary group 3 includes 1 liquid suction capillary and N liquid discharge capillaries; Wherein, N is the total number of buffer and dye types, M≥1, N≥1; the pipette table 4, the detachment rack 5, the recovery box 6, the test tube rack 7, the glass slide clamp 8 and the reagent bottle rack 10 are arranged on the lower layer of the support frame 1, and the pipette tip 2 can be inserted into the pipette placed on the pipette table 4 by interference fit; the detachment rack 5 can peel the pipette from the pipette tip 2 and peel it into the recovery box 6; the glass slide clamp 8 sets M glass slide grooves at the same X-axis coordinate, and the glass slide grooves correspond to the Y-axis coordinates of the capillary group 3 one by one; the sedimentation chamber 9 is a hollow cylindrical shape, and the sedimentation chamber 9 can press the glass slide and fix the glass slide on the glass slide groove; N groups of sample injection parts 11, N+1 double-tube clamp valves 12, M×N single-tube clamp valves 13, vacuum pump 14, waste The liquid tank 15 and the control panel 16 are arranged on the back of the support frame 1; each group of sample injection parts 11 includes a sample injection motor 1101 and a sampler 1102, and the sample injection motor 1101 can drive the sampler 1102 to perform piston movement; one of the samplers 1102 is connected to a hose, and the hose 1 is divided into 2 and passes through a double-tube clamp valve 12, and one hose is connected to the buffer solution placed on the reagent bottle rack 10, and the other hose is divided into 2 and passes through the double-tube clamp valve 12, and one hose is connected to the suction head 2, and the other hose 1 is divided into M, and each hose passes through a different single-tube clamp valve 13 and is connected to one of the discharge capillaries in each capillary group 3; the pipelines of the remaining N-1 samplers 1102 are connected The method is as follows: the sampler 1102 is connected to a hose, and the hose 1 is divided into 2 and passes through a double-tube clamp valve 12. One hose is connected to the dye placed on the reagent bottle rack 10, and the other hose 1 is divided into M. Each hose passes through a different single-tube clamp valve 13 and is connected to one of the discharge capillaries in each capillary group 3; the vacuum pump 14 is connected to the waste liquid tank 15 through a hose, and the waste liquid tank 15 is connected to the suction capillaries in all capillary groups 3 through a hose; the display screen 17 is arranged on the top cover of the support frame 1, and the display screen 17 is connected to the control panel 16. Through the mutual cooperation of various components, the entire process of cell preparation and staining of multiple slides can be automatically completed at the same time, effectively improving the efficiency of cell preparation.

[0034] As one of the embodiments, the moving assembly includes an X-axis translation assembly 18, a Y-axis translation assembly 19, a Z1-axis translation assembly 20 and a Z2-axis translation assembly 21; the X-axis translation assembly 18 includes an X-axis linear guide, an X-axis slider and an X-motor, the X-axis linear guide is installed on the upper layer of the support frame, and the X-motor can drive the X-axis slider to slide in the X-axis linear guide; the Y-axis translation assembly 19 includes a Y-axis mounting plate, a Y-axis linear guide, a Y-axis slider and a Y-motor, the Y-axis mounting plate is installed on the X-axis slider, the Y-axis linear guide is installed on the Y-axis mounting plate, and the Y-motor can drive the Y-axis slider to slide in the Y-axis linear guide; the Z1-axis translation assembly 20 includes a Z1-axis housing 2001, a Z1-axis Linear guide, Z1 slider and Z1 motor 2002, Z1 axis housing 2001 is connected with Y slider, Z1 axis linear guide is installed in Z1 axis housing 2001, Z1 motor 2102 can drive Z1 slider to slide in Z1 axis linear guide, and suction head 2 is connected with Z1 slider; Z2 translation assembly 21 includes Z2 axis housing 2101, Z2 axis linear guide, Z2 slider and Z2 motor 2102, Z2 axis housing 2101 is connected with Y axis mounting plate, Z2 axis linear guide is installed in Z2 axis housing 2101, Z2 motor 2102 can drive Z2 slider to slide in Z2 axis linear guide, and M capillary groups 3 are connected with Z2 slider.

[0035] Furthermore, in one embodiment, the cell production equipment 1000 also includes four adjustable foot cups 22, and the four adjustable foot cups 22 are installed at the bottom of the cell production equipment 1000. By installing the adjustable foot cups 22 at the bottom of the cell production equipment 1000, the cell production equipment 1000 can be supported and cushioned. In addition, the overall height of the cell production equipment 1000 can also be adjusted.

[0036] In one embodiment, the cell preparation device 1000 further includes a waste liquid tank 23, which is disposed at the lower layer of the support frame 1. The waste liquid tank 23 can be used to recover various waste liquids generated by the cleaning pipeline.

[0037] It should be understood that the specific embodiments described herein are only used to explain the application, rather than to limit it. Based on the embodiments provided in this application, all other embodiments obtained by ordinary technicians in this field without creative work are within the protection scope of this application.

[0038] Obviously, the drawings are only some examples or embodiments of the present application. For ordinary technicians in the field, the present application can also be applied to other similar situations based on these drawings without creative work. In addition, it is understandable that although the work done in this development process may be complicated and lengthy, for ordinary technicians in the field, certain changes in design, manufacturing or production based on the technical content disclosed in this application are only conventional technical means and should not be regarded as insufficient content disclosed in this application.

[0039] The term "embodiment" in this application refers to a specific feature, structure or characteristic described in conjunction with the embodiment that can be included in at least one embodiment of the present application. The appearance of this phrase in various locations in the specification does not necessarily mean the same embodiment, nor does it mean that it is mutually exclusive with other embodiments and is independent or optional. It is clearly or implicitly understood by those of ordinary skill in the art that the embodiments described in this application can be combined with other embodiments without conflict.

[0040] The above embodiments only express several implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of patent protection. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present application, and these all belong to the scope of protection of the present application. Therefore, the scope of protection of the present application shall be subject to the attached claims.

Claims

1. A cell preparation device, characterized in that: It includes a support frame, a pipette tip, M capillary groups, a moving component, a pipette table, a detachment frame, a recovery box, a test tube rack, a slide clamp, a sedimentation chamber, a reagent bottle rack, a sample injection part, a double-tube pinch valve, a single-tube pinch valve, a vacuum pump, a waste liquid tank, a control panel and a display screen; the pipette tip, the capillary group and the moving component are installed on the upper layer of the support frame, the moving component can drive the pipette tip to move along the X direction, the Y direction and the Z direction, the moving component can drive the capillary group to move along the X direction and the Z direction, and each capillary group includes 1 liquid suction capillary and N liquid discharge capillaries; wherein N is the total number of buffer and dye types, M≥1, N≥1; The pipette table, the detachment rack, the recovery box, the test tube rack, the glass slide clamp and the reagent bottle rack are arranged in the lower layer of the support frame, and the pipette head can be inserted into the pipette on the pipette table by interference fit; the detachment rack can peel the pipette from the pipette head and peel it into the recovery box; the glass slide clamp is provided with M glass slide grooves at the same X-axis coordinate, and the glass slide grooves correspond to the Y-axis coordinates of the capillary group one by one; the sedimentation chamber is a hollow cylindrical shape, and the sedimentation chamber can press the glass slide and fix the glass slide on the glass slide groove; N groups of the sample injection parts, N+1 double-tube pinch valves, M×N single-tube pinch valves, the vacuum pump, the waste liquid tank and the control panel are arranged on the back of the support frame; each group of the sample injection parts includes a sample injection motor and a sampler, and the sample injection motor can drive the sampler to perform piston movement; one of the samplers is connected to a hose, and the hose 1 is divided into 2 and passes through the double-tube pinch valve, and 1 hose is connected to the buffer solution placed on the reagent bottle rack, and the other hose is divided into 2 and passes through the double-tube pinch valve, and 1 hose is connected to the suction head, and the other hose is divided into M, and each hose passes through different single-tube pinch valves and is connected to each of the hairs One of the drainage capillaries in the capillary group is connected; the pipeline connection method of the remaining N-1 samplers is as follows: the sampler is connected with a hose, the hose 1 is divided into 2 and passes through a double-tube clamp valve, one hose is connected to the dye placed on the reagent bottle rack, and the other hose 1 is divided into M, each hose passes through a different single-tube clamp valve and is connected to one of the drainage capillaries in each capillary group; the vacuum pump is connected to the waste liquid tank through a hose, and the waste liquid tank is connected to the suction capillaries in all capillary groups through a hose; the display screen is arranged on the top cover of the support frame, and the display screen is connected to the control panel.

2. The cell preparation device according to claim 1, characterized in that: The moving assembly includes an X-axis translation assembly, a Y-axis translation assembly, a Z1-axis translation assembly, and a Z2-axis translation assembly; the X-axis translation assembly includes an X-axis linear guide, an X-axis slider, and an X-motor, the X-axis linear guide is installed on the upper layer of the support frame, and the X-motor can drive the X-axis slider to slide in the X-axis linear guide; the Y-axis translation assembly includes a Y-axis mounting plate, a Y-axis linear guide, a Y-axis slider, and a Y-motor, the Y-axis mounting plate is installed on the X-axis slider, the Y-axis linear guide is installed on the Y-axis mounting plate, and the Y-motor can drive the Y-axis slider to slide in the Y-axis linear guide; The Z1-axis translation component includes a Z1-axis housing, a Z1-axis linear guide, a Z1-axis slider and a Z1 motor, the Z1-axis housing is connected to the Y-axis slider, the Z1-axis linear guide is installed in the Z1-axis housing, the Z1 motor can drive the Z1-axis slider to slide in the Z1-axis linear guide, and the suction head is connected to the Z1-axis slider; the Z2-axis translation component includes a Z2-axis housing, a Z2-axis linear guide, a Z2-axis slider and a Z2 motor, the Z2-axis housing is connected to the Y-axis mounting plate, the Z2-axis linear guide is installed in the Z2-axis housing, the Z2 motor can drive the Z2-axis slider to slide in the Z2-axis linear guide, and the M capillary groups are connected to the Z2-axis slider.

3. The cell preparation device according to claim 1 or 2, characterized in that: The cell preparation device also includes four adjustable foot cups, which are installed at the bottom of the cell preparation device.

4. The cell preparation device according to claim 1 or 2, characterized in that: The cell preparation equipment also includes a waste liquid tank, which is arranged at the lower layer of the support frame and is used to recycle waste liquid.

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