Typing analysis method for extracellular vesicles secreted by tumor cells and immune cells

Through the dual-switching strategy of dual aptamers and molecular beacons, the identification of tumor cells and immune cells surface markers is solved, and the problem of difficulty in typing and analyzing extracellular vesicles in the prior art is achieved, efficient typing analysis of extracellular vesicles from different cell sources is enhanced, and the understanding of their biological significance is enhanced.

CN119688986AActive Publication Date: 2025-03-25XIAMEN UNIV
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Patent Information

Application Number
CN202411935510.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-26
Publication Date
2025-03-25
Estimated Expiration
2044-12-26

AI Technical Summary

Technical Problem

The prior art is difficult to effectively type and analyze extracellular vesicles from different cell sources, resulting in limited understanding of the biological significance of extracellular vesicles in intercellular communication.

Method used

A dual-switching strategy of dual aptamers and molecular beacons is used to identify tumor cells and immune cell surface-specific markers PD-L1 and EpCAM, combined with fluorescence identification technology, to distinguish tumor cells and immune cell-derived extracellular vesicles.

Benefits of technology

Efficient typing analysis of extracellular vesicles from different cell sources is achieved, false positive signals caused by a single aptamer are avoided, and understanding of the biological function information of extracellular vesicles is enhanced.

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Abstract

The invention discloses a typing analysis method for extracellular vesicles secreted by tumor cells and immune cells, which comprises the following steps: designing a PD-L1 protein recognition aptamer PD-L1-L, an EpCAM protein recognition aptamer EpCAM-L and a molecular beacon according to different expression levels of surface markers of the extracellular vesicles (PD-L1 + and EpCAM +) derived from the tumor cells and the extracellular vesicles (PD-L1 + and EpCAM-) derived from the immune cells; when the DNA probe recognizes the extracellular vesicles, fluorescence of the Cy5-PD-L1-L aptamer of the tumor cell source extracellular vesicles is quenched, a fluorescence signal of the Connector is recovered, and the immune cell source extracellular vesicles do not respond, so that the two extracellular vesicles are subjected to typing analysis. The method has the advantages that the extracellular vesicles of different cell sources can be subjected to typing analysis by using a strategy based on double aptamers and double switches, and false positive signals caused by single aptamer recognition are avoided.
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