Cryogenic treatment of cryoprotected tissue
By exposing the tissue sample to DMSO solution, freezing in liquid nitrogen, freezing and replacing it and adding embedded resin, the problem of difficulty in freezing and protecting larger tissue blocks in the prior art is solved, and high-quality ultrastructure maintenance and imaging of larger samples are achieved.
Patent Information
- Application Number
- CN202380057193.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2022-08-02
- Filing Date
- 2023-07-31
- Publication Date
- 2025-03-25
AI Technical Summary
The prior art is difficult to effectively freeze and protect larger tissue blocks, limiting the application of imaging methods such as photoelectric correlation microscopy (CLEM) and immunogold electron microscopy (iEM), especially when larger samples are processed while maintaining ultrastructure.
The tissue sample is frozen in liquid nitrogen by exposing it to a dimethyl sulfoxide (DMSO) solution, frozen in liquid nitrogen, frozen and replaced, and the embedded resin is added at low temperature to polymerize it, thereby achieving cryoprotection of the tissue sample.
This method allows the preparation and analysis of larger, thicker samples, maintaining ultrastructure, reducing dependence on vibration slicing and high-pressure fixation equipment, reducing costs, and improving the quality of imaging and comprehensive information.