Method for improving survival rate of phoebe formosana seedling after transplanting

Abstract

The application discloses a method for improving the survival rate of Phoebe sheareraiana large seedlings and belongs to the technical field of Phoebe sheareraiana planting, which comprises the steps of seed sand storage, sowing and seedling raising, root breaking treatment and transplanting, etc. After sowing, the Phoebe sheareraiana is transplanted into a container and is placed in the forest for shade seedling raising, and after being cultivated into large seedlings, the roots are broken for transplanting, and a root growth regulator is applied during the transplanting process. The root growth regulator can induce the roots of the Phoebe sheareraiana large seedlings to spread around and grow well after the roots are broken, solves the problem that the roots of the Phoebe sheareraiana container large seedlings are entrenched and entangled, and further inhibits the absorption of water and nutrients and the respiratory metabolism after transplanting, thereby reducing the survival rate of the Phoebe sheareraiana large seedlings and affecting the later growth of the Phoebe sheareraiana seedlings.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of Phoebe shearera, and particularly relates to a method for improving the survival rate of transplanted Phoebe shearera seedlings. BACKGROUND

[0002] Phoebe shearera, also known as Phoebe shearera, is a evergreen tree of Lauraceae, and is a national second-class protected endangered plant. Phoebe shearera has thin branches, straight trunk, and is covered with gray-brown or gray-yellow soft hair. The wood is hard and dense, has beautiful texture, is resistant to insect and corrosion, and has special fragrance. Phoebe shearera is a valuable and high-value timber afforestation tree species and a valuable landscaping tree species.

[0003] Container seedling is a way of seed or branch propagation materials in various containers filled with culture medium. Compared with ordinary land cultivation, container seedling has the advantages of short seedling period, convenient water and soil management, high seedling quality, and short hardening period, and is a widely used seedling production technology in the world. However, container seedlings have the disadvantages of weak resistance to diseases and pests and high cost, and therefore it is more suitable to cultivate large seedlings and then transplant them in actual production. In the process of container seedling, the root system forms a complete root group with developed fibrous roots. In the conventional method, the seedlings are directly transplanted with the soil group. However, Phoebe shearera is a multi-root tree species, and the developed root system is intertwined in the container to form a complex root group. The close arrangement of the root system inhibits the oxygen flow and affects the absorption of water and nutrients by the root system, thereby reducing the survival rate of seedling transplantation and leading to poor growth of the seedlings after transplantation.

[0004] Therefore, it is necessary to find a method suitable for the transplantation of Phoebe shearera seedlings to solve the problems of the reduced survival rate of seedling transplantation and the poor growth of seedlings after transplantation due to the intertwined root system, the close arrangement of the root system, the inhibition of oxygen flow, and the influence on the absorption of water and nutrients by the root system. SUMMARY

[0005] In view of this, the purpose of the present application is to provide a method for improving the survival rate of transplanted Phoebe shearera seedlings, which solves the problems of the reduced survival rate of seedling transplantation and the poor growth of seedlings after transplantation due to the intertwined root system, the close arrangement of the root system, the inhibition of oxygen flow, and the influence on the absorption of water and nutrients by the root system.

[0006] The present application solves the above technical problems by the following technical means:

[0007] A method for improving the survival rate of transplanted Phoebe shearera seedlings, the method comprising the following steps:

[0008] (1) Seed sand storage: After the fruit of Michelia macclurei is harvested, the seed is obtained by removing the pericarp in a wooden barrel, and then the seed is washed with water and dried. Then the seed is mixed with river sand in a mass ratio of 3:1, and the sand storage is carried out. During the sand storage, water is poured to keep the water content of the river sand at 60%.

[0009] (2) Seedling cultivation: In late March to early April, the soil in the greenhouse is ploughed and raked, and then the pretreated soil is obtained by applying compound fertilizer in an amount of 80 kg / m 2 . The sand-stored seeds are taken out, disinfected in 0.3wt% potassium permanganate solution for 15-30 min, and then washed with water for 2-3 times to obtain pretreated seeds. The pretreated seeds are sown in rows in an amount of 22-30 g / m 2 , the row spacing is 15-20 cm, the sowing depth is 1-2 cm, and the conventional method is used for pipe protection until 3-4 true leaves grow, and then the seedlings are transplanted into containers filled with substrate, poured with water, and placed in the shade for cultivation. Watering, fertilization and pest control are carried out according to the conventional method, and the container is replaced once a year, and the Michelia macclurei seedlings are obtained after 3-5 years of cultivation.

[0010] (3) Root cutting treatment: the Michelia macclurei seedlings are taken out of the container and subjected to root cutting treatment.

[0011] (4) Transplanting: dig a planting hole, mix the excavated soil with a root agent and calcium peroxide to obtain a mixed soil, backfill the mixed soil into the planting hole to a thickness of 8-12 cm, then vertically stand the root-cut Michelia macclurei seedlings in the planting hole, use hands to straighten the seedlings, continue to backfill the remaining mixed soil and compact it, then apply 0.5 kg of 50 mg / L indole acetic acid solution per plant by root irrigation method, and then pipe protection is carried out according to the conventional method.

[0012] Further, the substrate in the container in step (2) is obtained by mixing peat soil, yellow heart soil and calcium magnesium phosphate fertilizer in a mass ratio of (35-40):(40-50):(1-2).

[0013] Further, when the container is shaded for cultivation in step (2), the forest canopy density is 0.5-0.6.

[0014] Further, the root cutting operation in step (3) is as follows:

[0015] The Michelia macclurei seedlings are taken out of the container, and then 5-10 cm wide root system is cut along the root mass for transplanting.

[0016] Further, the mass ratio of soil to root agent and calcium peroxide in the mixed soil in step (4) is (15-20):(2-3):(0.1-0.2).

[0017] Further, the root agent comprises the following raw materials:

[0018] Chitosan, 2wt% isopropanol solution, 1,2-nonanediol, diglycolamine, 0.4wt% hydrochloric acid solution, β-glycerophosphate sodium, ammonium bicarbonate, superphosphate, potassium sulfate.

[0019] Further, the preparation method of the root growth agent is as follows:

[0020] A: Chitosan is added into 2wt% isopropanol solution and stirred and dispersed, 1,2-nonanediol is added after standing at room temperature for 2-4h, the temperature is increased to 40-50℃ for reaction for 1-2h, the reaction is completed, then cooled to room temperature and stand overnight, then adjust the pH to 4.5-5.5, add diglycolamine, stir and react at room temperature for 2-4h, filter the filtrate after the reaction is completed, and then dry to obtain treated chitosan;

[0021] B: The treated chitosan is added into 0.4wt% hydrochloric acid solution to obtain a chitosan solution; β-glycerophosphate sodium is dissolved in 4 times the mass of water to obtain a β-glycerophosphate sodium solution; the β-glycerophosphate sodium solution is slowly injected into the chitosan solution under the condition of 4℃ temperature, and stirred to obtain a sol solution;

[0022] C: Ammonium bicarbonate, superphosphate and potassium sulfate are mixed to obtain a mixed fertilizer, then the mixed fertilizer is added into 3 times the mass of water and stirred and dissolved to obtain a mixed fertilizer solution; the mixed fertilizer solution is added into the sol solution and stirred uniformly to obtain a root growth agent, which is stored at 4℃ for use.

[0023] After the large seedlings of Phoebe sheareri are cut off, they are transplanted, the root system of the seedlings has the properties of tropism to fertilizer, water and oxygen, a root growth agent is prepared and mixed into the soil with calcium peroxide, the root growth agent and calcium peroxide jointly induce the seedlings after cutting to grow towards the distribution area of the root growth agent. Specifically, the root growth agent is prepared by reacting chitosan as a carrier with β-glycerophosphate sodium to form a sol solution with temperature sensitivity, and ammonium bicarbonate, superphosphate and other fertilizers are added, the root growth agent will gelate to form a gel wrapping soil particles after being mixed into the soil due to the temperature sensitivity, and has good water retention, and then the backfill soil mixed with the root growth agent can continuously provide water and nutrients, and the added calcium peroxide continuously hydrolyzes to release oxygen under the action of water in the gel, and then the root system of the Phoebe sheareri seedlings cultivated in containers is induced to stretch in multiple directions and grow well towards the soil position of the root growth agent after cutting, the absorption of nutrients and water and the flow of oxygen of the root system of the seedlings after transplanting are promoted, and then the survival rate of the seedlings after transplanting and the growth condition in the later period are ensured.

[0024] However, the sol-gel prepared by chitosan and sodium beta-glycerophosphate has a high gelation temperature, and when the soil temperature is low, the nutrients in the sol-gel cannot be gelated, resulting in loss of nutrients and poor water retention. Therefore, the present application uses 1,2-nonanediol to react with chitosan, which is combined with the chitosan molecular chain to change the intermolecular interaction force to promote the cross-linking polymerization of the sol solution, thereby reducing the gelation temperature, so that the sol-gel can be successfully gelated in the soil at a lower temperature and then wrapped around the soil particles to continuously provide water and nutrients.

[0025] In addition, in order to make the root growth regulator uniformly mixed with the soil particles and embedded therein, the present application also uses diglycolamine to react with chitosan to change the charge properties of the chitosan to regulate the proton transfer ability of the chitosan gel when it is gelated in the soil, thereby delaying the formation of chemical bonds in the gel network, prolonging the gelation time, and allowing the chitosan sol solution to better penetrate into the soil particles before being gelated and solidified, ensuring the uniformity of the distribution of the root growth regulator, so that the roots can grow in multiple directions towards the areas where the root growth regulator is distributed, thereby promoting the growth of the roots to ensure the survival and growth of the seedlings.

[0026] Further, in the step A, the mass ratio of chitosan, 2wt% isopropyl alcohol solution, 1,2-nonanediol, and diglycolamine is 0.2:2:(0.001-0.003):(0.002-0.006).

[0027] Further, in the step B, the mass ratio of pretreated chitosan, 0.4wt% hydrochloric acid solution, and sodium beta-glycerophosphate solution is 0.1:5:3.

[0028] Further, in the step C, the mass ratio of ammonium bicarbonate, superphosphate, potassium sulfate, and sol solution is (0.2-0.4):(0.1-0.2):(0.1-0.2):8.

[0029] Beneficial effects:

[0030] The present application uses chitosan as a raw material to prepare a root growth regulator that can be gelated and has temperature sensitivity, adds ammonium bicarbonate and other fertilizers to the root growth regulator, mixes the root growth regulator and calcium peroxide into the soil when the large seedlings of Phoebe sheareri are transplanted, forms a gel that wraps around the soil particles by gelation of the root growth regulator, and backfills the mixed soil into the planting hole. The seedlings are induced to grow towards the areas where the root growth regulator is distributed in the surrounding soil by the seedlings' tropism to fertilizer, water, and oxygen, and the roots grow in multiple directions. After the entangled roots are broken, they can grow better to ensure the absorption of water and nutrients and the circulation of oxygen in the roots of the seedlings after transplantation, improve the survival rate of the transplanted seedlings, and ensure the good growth of the seedlings after transplantation. BRIEF DESCRIPTION OF DRAWINGS

[0031] Figure 1: It is the picture of the undergrowth cultivation condition of the Michelia macclurei seedling in the embodiment 4 of the present application.

[0032] Figure 2 : It is the picture of the Michelia macclurei large seedling transplanted in the experimental group 1 of the present application. DETAILED DESCRIPTION

[0033] The present application will be described in detail below in combination with specific embodiments and drawings:

[0034] Embodiment 1: Preparation of root growth agent one

[0035] A: 0.2kg chitosan is added into 2kg 2wt% isopropyl alcohol solution to be stirred and dispersed, and then 0.002kg 1,2-nonanediol is added after standing at room temperature for 3h, and the temperature is increased to 45℃ to react for 1.5h, and then the reaction is completed to cool to room temperature to stand overnight, and then 0.004kg diglycolamine is added after adjusting pH to 5, and then the reaction is stirred at normal temperature for 3h, and then the reaction is completed to filter to remove the filtrate, and then the treated chitosan is obtained by drying at 60℃;

[0036] B: 0.1kg treated chitosan is added into 5kg 0.4wt% hydrochloric acid solution to be dissolved to obtain chitosan solution; β-glycerophosphate sodium is added into 4 times mass of water to be dissolved to obtain β-glycerophosphate sodium solution; 3kg β-glycerophosphate sodium solution is slowly injected into the chitosan solution under the temperature condition of 4℃ to be stirred and reacted to obtain sol solution;

[0037] C: 0.3kg ammonium bicarbonate, 0.15kg superphosphate and 0.15kg potassium sulfate are mixed to obtain mixed fertilizer, and then the mixed fertilizer is added into 1.8kg water to be stirred and dissolved to obtain mixed fertilizer solution; the mixed fertilizer solution is added into 8kg sol solution to be stirred uniformly to obtain root growth agent, which is stored at 4℃ for use.

[0038] Embodiment 2: Preparation of root growth agent two

[0039] A: 0.2kg chitosan is added into 2kg 2wt% isopropyl alcohol solution to be stirred and dispersed, and then 0.001kg 1,2-nonanediol is added after standing at room temperature for 2h, and the temperature is increased to 40℃ to react for 2h, and then the reaction is completed to cool to room temperature to stand overnight, and then 0.002kg diglycolamine is added after adjusting pH to 4.5, and then the reaction is stirred at normal temperature for 2h, and then the reaction is completed to filter to remove the filtrate, and then the treated chitosan is obtained by drying at 60℃;

[0040] B: 0.1kg treated chitosan is added into 5kg 0.4wt% hydrochloric acid solution to be dissolved to obtain chitosan solution; β-glycerophosphate sodium is added into 4 times mass of water to be dissolved to obtain β-glycerophosphate sodium solution; 3kg β-glycerophosphate sodium solution is slowly injected into the chitosan solution under the temperature condition of 4℃ to be stirred and reacted to obtain sol solution;

[0041] C: 0.2 kg of ammonium bicarbonate, 0.1 kg of superphosphate, 0.1 kg of potassium sulfate are mixed to obtain a mixed fertilizer, and the mixed fertilizer is dissolved in 1.2 kg of water to obtain a mixed fertilizer solution; the mixed fertilizer solution is added to 8 kg of sol solution and stirred uniformly to obtain a root agent, which is stored at 4°C for later use.

[0042] Example 3: Preparation of root agent

[0043] A: 0.2 kg of chitosan is added to 2 kg of 2 wt% isopropyl alcohol solution and stirred to disperse, and then 0.003 kg of 1,2-nonanediol is added after standing at room temperature for 4 h, and the temperature is increased to 50°C for reaction for 1 h, and then the reaction is cooled to room temperature overnight, and then the pH is adjusted to 5.5, and 0.006 kg of diglycolamine is added, and stirred at room temperature for 4 h, and then the filtrate is removed after filtration, and then dried at 60°C to obtain treated chitosan;

[0044] B: 0.1 kg of treated chitosan is added to 5 kg of 0.4 wt% hydrochloric acid solution to obtain a chitosan solution; β-glycerophosphate sodium is dissolved in 4 times the mass of water to obtain a β-glycerophosphate sodium solution; under the condition of 4°C, 3 kg of β-glycerophosphate sodium solution is slowly injected into the chitosan solution and stirred to obtain a sol solution;

[0045] C: 0.4 kg of ammonium bicarbonate, 0.2 kg of superphosphate, 0.2 kg of potassium sulfate are mixed to obtain a mixed fertilizer, and the mixed fertilizer is dissolved in 2.4 kg of water to obtain a mixed fertilizer solution; the mixed fertilizer solution is added to 8 kg of sol solution and stirred uniformly to obtain a root agent, which is stored at 4°C for later use.

[0046] Comparative Example 1: Preparation of root agent

[0047] In comparison with Example 1, the only difference is that no 1,2-nonanediol is added in step A when the root agent is prepared in Comparative Example 1, which is shown as follows:

[0048] A: 0.2 kg of chitosan is added to 2 kg of 2 wt% isopropyl alcohol solution and stirred to disperse, and then 0.003 kg of 1,2-nonanediol is added after standing at room temperature for 4 h, and the temperature is increased to 50°C for reaction for 1 h, and then the reaction is cooled to room temperature overnight, and then the pH is adjusted to 5.5, and 0.006 kg of diglycolamine is added, and stirred at room temperature for 4 h, and then the filtrate is removed after filtration, and then dried at 60°C to obtain treated chitosan;

[0049] B-C: Same as Example 1.

[0050] Comparative Example 2: Preparation of root agent

[0051] In comparison with Example 1, the only difference is that no diglycolamine is added in step A when the root agent is prepared in Comparative Example 2, which is shown as follows:

[0052] A: 0.2 kg chitosan was added into 2 kg 2 wt% isopropyl alcohol solution and stirred to disperse, and then 0.002 kg 1,2-nonanediol was added after standing at room temperature for 3 h, and the temperature was increased to 45 °C for reaction for 1.5 h. After the reaction was completed, the temperature was cooled to room temperature and stood overnight, and then the filtrate was removed by filtration, and the treated chitosan was obtained by drying at 60 °C;

[0053] B-C: the same as Example 1.

[0054] Preparation of root growth regulator

[0055] In comparison with Example 1, the only difference is that the original step A is absent in the preparation of the root growth regulator in Comparative Example 3, that is, the chitosan is not treated, but a conventional chitosan is used, and the preparation is as follows:

[0056] A: 0.1 kg chitosan was dissolved in 5 kg 0.4 wt% hydrochloric acid solution to obtain a chitosan solution; sodium β-glycerophosphate was dissolved in 4 times the mass of water to obtain a sodium β-glycerophosphate solution; 3 kg of the sodium β-glycerophosphate solution was slowly injected into the chitosan solution under the condition of 4 °C temperature, and the sol solution was obtained by stirring and reaction;

[0057] B: 0.3 kg ammonium bicarbonate, 0.15 kg superphosphate, and 0.15 kg potassium sulfate were mixed to obtain a mixed fertilizer, and the mixed fertilizer was dissolved in 1.8 kg water to obtain a mixed fertilizer solution; the mixed fertilizer solution was added to 8 kg of the sol solution to obtain a root growth regulator, which was stored at 4 °C for use.

[0058] Preparation of root growth regulator

[0059] In comparison with Example 1, the only difference is that the pH is adjusted to 3.5 in step A in the preparation of the root growth regulator in Comparative Example 4, and the preparation is as follows:

[0060] A: 0.2 kg chitosan was added into 2 kg 2 wt% isopropyl alcohol solution and stirred to disperse, and then 0.002 kg 1,2-nonanediol was added after standing at room temperature for 3 h, and the temperature was increased to 45 °C for reaction for 1.5 h. After the reaction was completed, the temperature was cooled to room temperature and stood overnight, and then the filtrate was removed by filtration, and the treated chitosan was obtained by drying at 60 °C;

[0061] B-C: the same as Example 1.

[0062] Preparation of root growth regulator

[0063] In comparison with Example 1, the only difference is that the pH is adjusted to 6.5 in step A in the preparation of the root growth regulator in Comparative Example 5, and the preparation is as follows:

[0064] A: 0.2 kg of chitosan was added into 2 kg of 2 wt% isopropanol solution and stirred to disperse, and then 0.002 kg of 1,2-nonanediol was added after standing at room temperature for 3 h, and the reaction was carried out at 45 °C for 1.5 h, and then the reaction was completed after cooling to room temperature and standing overnight, and then 0.004 kg of diglycolamine was added after adjusting the pH to 6.5, and the reaction was carried out at room temperature for 3 h, and then the filtrate was removed after filtration, and then 60 °C drying was carried out to obtain the treated chitosan;

[0065] B-C: the same as example 1.

[0066] Preparation of a root growth regulator

[0067] In comparison with example 1, the only difference is that the amount of the pretreated chitosan in step B in the preparation of the root growth regulator in comparative example 6 is 0.05 kg, and the specific process is as follows:

[0068] A: the same as example 1;

[0069] B: 0.05 kg of the treated chitosan was added into 5 kg of 0.4 wt% hydrochloric acid solution to obtain a chitosan solution; 3 kg of β-glycerophosphate sodium was dissolved in 4 times the mass of water to obtain a β-glycerophosphate sodium solution; 3 kg of the β-glycerophosphate sodium solution was slowly injected into the chitosan solution under the condition of 4 °C to obtain a sol solution;

[0070] C: the same as example 1.

[0071] Preparation of a root growth regulator

[0072] In comparison with example 1, the only difference is that the amount of the pretreated chitosan in step B in the preparation of the root growth regulator in comparative example 7 is 0.15 kg, and the specific process is as follows:

[0073] A: the same as example 1;

[0074] B: 0.15 kg of the treated chitosan was added into 5 kg of 0.4 wt% hydrochloric acid solution to obtain a chitosan solution; 3 kg of β-glycerophosphate sodium was dissolved in 4 times the mass of water to obtain a β-glycerophosphate sodium solution; 3 kg of the β-glycerophosphate sodium solution was slowly injected into the chitosan solution under the condition of 4 °C to obtain a sol solution;

[0075] C: the same as example 1.

[0076] Preparation of a root growth regulator

[0077] In comparison with example 1, the only difference is that the amount of the β-glycerophosphate sodium solution in the preparation of the root growth regulator in comparative example 8 is 2 kg, and the specific process is as follows:

[0078] A: the same as example 1;

[0079] B: 0.1 kg of treated chitosan was added into 5 kg of 0.4 wt% hydrochloric acid solution to dissolve and obtain a chitosan solution; β-glycerophosphate sodium was added into 4 times of mass of water to dissolve and obtain a β-glycerophosphate sodium solution; 2 kg of the β-glycerophosphate sodium solution was slowly injected into the chitosan solution under the condition of 4 °C to stir and react to obtain a sol solution;

[0080] C: the same as example 1.

[0081] Comparative example 9: preparation of a root growth agent

[0082] In contrast to example 1, the only difference is that in comparative example 9, the amount of β-glycerophosphate sodium solution added during the preparation of the root growth agent is 4 kg, as shown below:

[0083] A: the same as example 1;

[0084] B: 0.1 kg of treated chitosan was added into 5 kg of 0.4 wt% hydrochloric acid solution to dissolve and obtain a chitosan solution; β-glycerophosphate sodium was added into 4 times of mass of water to dissolve and obtain a β-glycerophosphate sodium solution; 4 kg of the β-glycerophosphate sodium solution was slowly injected into the chitosan solution under the condition of 4 °C to stir and react to obtain a sol solution;

[0085] C: the same as example 1.

[0086] Example 4: method for transplanting large Phoebe sheareri seedlings

[0087] (1) seed sand storage: after the fruits of Phoebe sheareri were harvested, the seeds were obtained by removing the pericarp in a wooden barrel, the seeds were washed with water and then dried, then the seeds were mixed with river sand at a mass ratio of 3:1 for sand storage, and water was poured during the sand storage to maintain the water content of the river sand at about 60%;

[0088] (2) sowing and seedling raising: in early April, the soil in the greenhouse was ploughed and raked, 80 kg / m 2 of compound fertilizer was applied to obtain pretreated soil; the sand-stored seeds were taken out, disinfected in 0.3 wt% potassium permanganate solution for 20 min, and then washed with water for 3 times to obtain pretreated seeds; the pretreated seeds were sown at a rate of 25 g / m 2Sowing in rows with a row spacing of about 16 cm and a seeding depth of about 1.5 cm, and then tending according to a conventional method; transplanting after 3-4 true leaves have grown, specifically, first mixing peat soil, yellow heart soil, calcium-magnesium-phosphorus fertilizer in a mass ratio of 35:40:1 to obtain a substrate, then transplanting the seedlings into the container pots filled with the substrate at 1 pot per 1 seedling, placing them under the shade of a Chinese fir tree with a canopy density of about 0.5 for cultivation, watering, fertilizing, and pest control according to a conventional method, and replacing the container pots at a frequency of once a year to obtain the Machilus pauhoi seedlings after 4 years of cultivation;

[0089] (3) Root cutting treatment: taking the Machilus pauhoi seedlings out of the container pots, and then cutting about 6 cm wide of the root system along the root ball for transplanting;

[0090] (4) Transplanting: preparing a root growth agent according to the method of Example 1, then digging a planting hole, mixing the dug soil, the root growth agent, and calcium peroxide uniformly at a mass ratio of 15:3:0.2 to obtain mixed soil, backfilling the mixed soil into the planting hole at a thickness of about 10 cm, then vertically standing the root-cut Machilus pauhoi seedlings in the planting hole, holding the seedlings with hands, and continuing to backfill and compact the remaining mixed soil, then applying 0.5 kg of 50 mg / L indole acetic acid solution to each plant according to the root irrigation method, and tending according to a conventional method.

[0091] Comparative Example 10: Machilus pauhoi seedling transplanting method

[0092] In comparison with Example 4, the only difference is that the Machilus pauhoi seedlings are not subjected to root cutting treatment in Comparative Example 10, and are directly transplanted, and the remaining steps are the same as those of Example 4.

[0093] Comparative Example 11: Machilus pauhoi seedling transplanting method

[0094] In comparison with Example 4, the only difference is that no root growth agent is mixed into the soil in Comparative Example 11, only calcium peroxide is mixed into the soil, and the remaining steps are the same as those of Example 4.

[0095] Comparative Example 12: Machilus pauhoi seedling transplanting method

[0096] In comparison with Example 4, the only difference is that no calcium peroxide is mixed into the soil in Comparative Example 12, and the remaining steps are the same as those of Example 4.

[0097] Comparative Example 13: Machilus pauhoi seedling transplanting method

[0098] In comparison with Example 4, the only difference is that no root cutting treatment is performed in Comparative Example 13, and the seedlings are directly taken out of the container for transplanting, and no root growth agent and calcium peroxide are mixed into the soil. The remaining steps are the same as those of Example 4.

[0099] Experiment 1: performance detection experiment of root growth agent

[0100] 1. The gelation time and gelation temperature of the root growth agent prepared in Example 1 and Comparative Examples 1-9 were detected, and the data are shown in Table 1:

[0101] Table 1

[0102] Gelation temperature (°C) Gelation time (s) Example 1 23 326 Comparative Example 1 29 312 Comparative Example 2 23 224 Comparative Example 3 32 209 Comparative Example 4 24 302 Comparative Example 5 25 326 Comparative Example 6 28 408 Comparative Example 7 25 347 Comparative Example 8 27 411 Comparative Example 9 26 375

[0103] According to the data analysis of Table 1, it can be known that:

[0104] (1) The gelation temperature of the root growth agent prepared in Example 1 is 23°C, and the gelation time is 326s, which indicates that the root growth agent prepared by the method of the present application can effectively gelate and wrap the soil in a lower environment, thereby fixing the nutrient, water and oxygen releasing substance calcium peroxide, continuously providing water, nutrients and oxygen, thereby inducing the root system to stretch in all directions, and the gelation time is relatively moderate, which can be uniformly dispersed in the soil during mixing with the soil to gelate and induce the root system to stretch in multiple directions.

[0105] (2) In Comparative Example 1, 1,2-nonanediol is not added during the preparation of the root growth agent, and the gelation temperature is high, which requires higher temperature conditions for application; in Comparative Example 2, diethylene glycol amine is not added during the preparation of the root growth agent, and the gelation time of the root growth agent is short, thereby affecting the uniformity of distribution in the soil during mixing with the soil and reducing the induction effect of the root growth agent on the root system.

[0106] Experiment 2: Phoebe bournei seedling transplanting experiment

[0107] Phoebe bournei seedling transplanting experiment was carried out in the national long-term scientific research base of Chongqing Academy of Forestry: and transplanting was carried out in early June, and the method of sowing and growing seedlings according to steps (1) and (2) in Example 4 was used to cultivate Phoebe bournei seedlings under the cedar forest, and after 4 years of cultivation, large seedlings with consistent growth conditions were selected for transplanting, and the experiment was divided into 14 groups: experimental group 1, control group 1-13, and the root growth agents and transplanting methods used in each group are as follows:

[0108] Experimental group 1: the root growth agent prepared by the method of Example 1 and the transplanting method of Example 4 were used;

[0109] Control groups 1-9: the root growth agents of Comparative Examples 1-9 and the transplanting method of Example 4 were used respectively;

[0110] Control group 10: the root growth agent of Example 1 and the transplanting method of Comparative Example 10 were used;

[0111] Control group 11: no root growth agent was used, and the transplanting method of Comparative Example 11 was used;

[0112] Control group 12: the transplanting method of Example 1 and Comparative Example 12;

[0113] Control group 13 (blank control): no application of the root growth agent, and the transplanting method of Comparative Example 13.

[0114] 2. Ten large Machilus pauhoi seedlings in each group were transplanted and the survival rate of each group was recorded, and the height increment and ground diameter increment of each group after one year of transplanting were recorded, and the average data are shown in Table 2:

[0115] Table 2

[0116]

[0117]

[0118] According to the data in Table 2, it can be seen that:

[0119] (1) The survival rate of Machilus pauhoi large seedlings in the experimental group 1 is high, reaching 100%, and the growth condition after transplanting is good. The survival rate of the control groups 1-12 and the experimental group 1 has little difference, but the height increment and ground diameter increment after transplanting have obvious difference, which shows that the cultivation and transplanting treatment of Machilus pauhoi large seedlings by the method of the present application can effectively promote the growth of the root system of Machilus pauhoi large seedlings, and further improve the growth condition of the plant, and promote the increase of the height and ground diameter.

[0120] (2) The survival rate and height increment of Machilus pauhoi seedlings in the blank control group are obviously poor, which shows that the root cutting treatment of Machilus pauhoi large seedlings according to the method of the present application, and mixing the root growth agent and calcium peroxide prepared by the present application into the soil can better ensure the survival rate of Machilus pauhoi large seedlings and promote the good growth of the seedlings after transplanting.

[0121] The above examples are only used to illustrate the technical solutions of the present application and are not limiting. Although the present application has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solutions of the present application can be modified or replaced by equivalents without departing from the spirit and scope of the present application, and they should be covered in the scope of the claims of the present application. The technical, shape and structure parts not described in detail in the present application are well-known technologies.

Claims

1. A method for improving the survival rate of large Phoebe zhennan seedlings during transplantation, characterized in that, The method is as follows: (1) Seed storage in sand: After the fruit of Phoebe zhennan is harvested, the pericarp is removed to obtain the seeds. The seeds are washed with water, dried and then stored in sand for later use. (2) Sowing and seedling raising: From the end of March to the beginning of April, the soil in the greenhouse is turned over and compound fertilizer is applied to obtain pretreated soil; The seeds stored in sand were taken out, disinfected in a 0.3wt% potassium permanganate solution, and then washed with water to obtain pretreated seeds. The pretreated seeds were sown in the pretreated soil by row sowing and managed according to conventional methods until 3-4 true leaves emerged. Then they were transplanted into containers filled with substrate and placed under the shade of the forest for cultivation. They were managed according to conventional methods and the containers were changed once a year. After 3-5 years of cultivation, large seedlings of Phoebe zhennan were obtained. (3) Root pruning treatment: After removing the large Phoebe zhennan seedlings from the container, root pruning treatment is performed; (4) Transplanting: After digging a planting hole, mix the excavated soil with root relaxant and calcium peroxide to obtain mixed soil. First, backfill the mixed soil into the planting hole to a thickness of 8-12cm. Then, place the root-cut Phoebe zhennan seedlings vertically in the planting hole, continue to backfill the remaining mixed soil and compact it. Then, apply 0.5kg of 50mg / L indoleacetic acid solution to each plant by root irrigation. After that, manage the plant according to the conventional method. The root-relieving agent comprises the following raw materials: Chitosan, 2wt% isopropanol solution, 1,2-nonanediol, diethylene glycolamine, 0.4wt% hydrochloric acid solution, sodium β-glycerophosphate, ammonium bicarbonate, superphosphate, potassium sulfate; The method for preparing the root-relieving agent is as follows: A: Chitosan was added to a 2wt% isopropanol solution and stirred to disperse. After standing at room temperature for 2-4 hours, 1,2-nonanediol was added, and the temperature was raised to 40-50℃ for 1-2 hours. After the reaction was completed, the mixture was cooled to room temperature and allowed to stand overnight. Then, the pH was adjusted to 4.5-5.5, and diethylene glycolamine was added. The mixture was stirred at room temperature for 2-4 hours. After the reaction was completed, the filtrate was filtered to remove the residue, and then dried to obtain the processed chitosan. B: Dissolve the treated chitosan in 0.4wt% hydrochloric acid solution to obtain a chitosan solution; dissolve sodium β-glycerophosphate in 4 times its mass of water to obtain a sodium β-glycerophosphate solution; slowly inject the sodium β-glycerophosphate solution into the chitosan solution at 4℃ and stir to obtain a sol solution. C: Mix ammonium bicarbonate, superphosphate, and potassium sulfate to obtain a mixed fertilizer. Then, add the mixed fertilizer to 3 times its weight of water and stir to dissolve it to obtain a mixed fertilizer solution. Add the mixed fertilizer solution to the sol solution and stir evenly to obtain a root relaxant. Store at 4°C for later use. In step A, the mass ratio of chitosan, 2wt% isopropanol solution, 1,2-nonanediol, and diethylene glycol amine is 0.2:2:(0.001~0.003):(0.002~0.006). In step B, the mass ratio of pretreated chitosan, 0.4wt% hydrochloric acid solution, and β-glycerophosphate sodium solution is 0.1:5:

3.

2. The method for improving the survival rate of large Phoebe zhennan seedlings after transplanting according to claim 1, characterized in that, In step (2), the substrate in the container is obtained by mixing peat soil, yellow soil and calcium magnesium phosphate fertilizer in a mass ratio of (35-40): (40-50): (1-2).

3. The method for improving the survival rate of large Phoebe zhennan seedlings after transplanting according to claim 2, characterized in that, In step (2), the canopy closure of the trees during the cultivation under the shade of the forest is 0.5 to 0.

6.

4. The method for improving the survival rate of large Phoebe zhennan seedlings after transplanting according to claim 3, characterized in that, The specific steps of interrupting the root operation in step (3) are as follows: Remove the large nanmu seedlings from the container, then cut off 5-10cm of the root system around the root ball before transplanting.

5. The method for improving the survival rate of large Phoebe zhennan seedlings after transplanting according to claim 4, characterized in that, In step (4), the mass ratio of soil to root relaxant and calcium peroxide in the mixed soil is (15-20):(2-3):(0.1-0.2).

6. The method for improving the survival rate of large Phoebe zhennan seedlings after transplanting according to claim 5, characterized in that, In step C, the mass ratio of ammonium bicarbonate, superphosphate, potassium sulfate, and sol is (0.2-0.4):(0.1-0.2):(0.1-0.2):8.

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