Light quality matching method for improving quality degree and rooting rate of asparagus cochinchinensis tissue culture seedlings

Abstract

The invention belongs to the technical field of seedling raising of traditional Chinese medicinal materials, and particularly relates to a light quality matching method for improving the quality degree and the rooting rate of radix asparagi tissue culture seedlings. Compared with the prior art that natural light is directly adopted or a conventional tissue culture LED lamp and the like are used as a light source, the method has the advantages that by improving the light quality ratio and the like of the radix asparagi in the proliferation stage and the rooting stage after proliferation, the propagation time of the leaves of the radix asparagi can be effectively shortened, so that the quality degree and the rooting rate of the tissue culture seedlings of the radix asparagi are improved.

Description

Technical Field

[0001] The invention belongs to the technical field of Chinese medicinal material seedling cultivation, and particularly relates to a light quality matching method for improving the lignification degree and rooting rate of asparagus cochinchinensis tissue culture seedlings. Background Art

[0002] Neijiang Asparagus is a local medicinal material and landmark product of Neijiang. It is very different from Asparagus produced in Guangxi, Yunnan and other production areas in terms of morphology, composition and propagation method. It has excellent quality, high content of polysaccharides, amino acids and extracts, low content of saponins, sweet taste and no bitterness in the mouth. It has a long history of consumption and broad application prospects. It is an important variety of Asparagus in the use of medicine and food, and has high potential for development of food and health products. In recent years, Neijiang City has vigorously developed the health industry of traditional Chinese medicine and created the "Hometown of Chinese Asparagus", which has promoted the inheritance and innovation of Neijiang traditional Chinese medicine. However, Neijiang Asparagus has low seed fruiting rate and germination rate. The traditional propagation method relies on division. It requires the mother plant to grow for 3-4 years and then propagate in the harvest season. Usually, each plant can reproduce 3-5 plants. The propagation time is long and the efficiency is low, which greatly limits the development of Neijiang Asparagus industry. The establishment of Neijiang Asparagus tissue culture rapid propagation technology can solve the urgent shortage of seedlings in production. However, the light source commonly used in the tissue culture of Neijiang Asparagus is white LED lamp. Under the same light intensity, the photon density is low, and there is currently no light source system developed for Neijiang Asparagus. Using the traditional lighting system and existing tissue culture technology, the degree of lignification of the Asparagus tissue culture seedlings before rooting is low, and more steps are required to strengthen the seedlings. The growth time is long and the rooting rate is low, which greatly increases the production cost of Neijiang Asparagus tissue culture seedlings and affects the development of the Asparagus industry. Summary of the invention

[0003] In order to overcome the above technical problems, the present application provides a light quality matching method for improving the lignification degree and rooting rate of Asparagus cochinchinensis tissue culture seedlings, by improving the light source of Asparagus cochinchinensis during the proliferation stage and shortening the time for the pseudo-leaf expansion of Asparagus cochinchinensis, thereby improving the lignification degree and rooting rate of Asparagus cochinchinensis tissue culture seedlings.

[0004] In order to achieve the above-mentioned purpose, the technical solution of the present application discloses a light quality matching method for improving the lignification degree and rooting rate of asparagus cochinchinensis tissue culture seedlings, comprising the following steps:

[0005] The following light quality combinations were used during the proliferation stage of asparagus tissue culture seedlings:

[0006] Wavelength 380-399nm: 0.05%-0.15%;

[0007] Wavelength 400-499nm: 20.5%-30.0%;

[0008] Wavelength 500-599nm: 30.05%-43.5%;

[0009] Wavelength 600-699nm: 29.05%-40.0%;

[0010] Wavelength 700-780nm: 1.50%-2.75%;

[0011] The following light quality combinations are used during the rooting stage of Asparagus:

[0012] Wavelength 380-399nm: 0.04%-0.14%;

[0013] Wavelength 400-499nm: 17.0%-25.0%;

[0014] Wavelength 500-599nm: 35.5%-43.5%;

[0015] Wavelength 600-699nm: 30.5%-41.5%;

[0016] Wavelength 700-780nm: 2.4%-4.0%.

[0017] Preferably, the light quality matching method comprises the following steps:

[0018] The following light quality combinations were used during the proliferation stage of asparagus tissue culture seedlings:

[0019] Wavelength 380-399nm: 0.07%-0.13%;

[0020] Wavelength 400-499nm: 22.36%-29.31%;

[0021] Wavelength 500-599nm: 36.6%-43.25%;

[0022] Wavelength 600-699nm: 29.36%-34.92%;

[0023] Wavelength 700-780nm: 1.84%-2.02%;

[0024] The following light quality combinations are used during the rooting stage of Asparagus:

[0025] Wavelength 380-399nm: 0.04%-0.10%;

[0026] Wavelength 400-499nm: 18.04%-24.16%;

[0027] Wavelength 500-599nm: 36.06%-42.98%;

[0028] Wavelength 600-699nm: 33.78%-39.8%;

[0029] Wavelength is 700-780nm: 2.33%-2.69%.

[0030] Furthermore, the light intensity in both the proliferation stage and the rooting stage is 2000-4000 lx.

[0031] Furthermore, the light quantum density in the multiplication stage is 90-140umol / m 2 / s.

[0032] Furthermore, the light quantum density in the rooting stage is 40-80umol / m 2 / s.

[0033] Furthermore, the red-to-blue light ratio of the asparagus tissue culture seedlings during the proliferation stage is 0.8-1.5, and the red light to far-red light ratio is 10.0-20.0.

[0034] Furthermore, the red-to-blue light ratio during the rooting stage of the asparagus is 2.0-3.0, and the red light to far-red light ratio is 7.0-15.0.

[0035] Compared with the prior art, the present invention has the following beneficial effects: by improving the light source intensity, light quality ratio, etc. of Asparagus cochinchinensis during the proliferation stage and the rooting stage after proliferation, the present application can effectively shorten the time for the pseudo-leaf expansion of Asparagus cochinchinensis compared with the prior art of directly using natural light or using white light as the light source, thereby improving the lignification degree and rooting rate of Asparagus cochinchinensis tissue culture seedlings. DETAILED DESCRIPTION

[0036] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present invention.

[0037] Unless otherwise specifically stated, the relative arrangement of the parts and steps set forth in these embodiments, numerical expressions and numerical values ​​do not limit the scope of the application. The technology, method and equipment known to those of ordinary skill in the relevant art may not be discussed in detail, but in appropriate cases, the technology, method and equipment should be considered as a part of the authorization specification. In all examples shown and discussed here, any specific value should be interpreted as being merely exemplary, rather than as a limitation. Therefore, other examples of exemplary embodiments may have different values.

[0038] Unless otherwise specified, the meanings of the technical terms in this specification are the same as those generally understood by those skilled in the art. However, if there is any conflict, the definitions in this specification shall prevail.

[0039] Example 1 Effects of different light quality combinations on germination and lignification of Neijiang Asparagus cochinchinensis tissue culture seedlings

[0040] Experimental method: The Neijiang Asparagus cochinchinensis proliferation seedlings were inoculated on the proliferation culture medium and cultured in LED lamps with different light qualities. Commonly used white light LED lamps were used as controls to observe the effects of different LED light sources on the growth indicators, growth status and lignification degree of the Neijiang Asparagus cochinchinensis proliferation seedlings. The proliferation culture medium formula was: MS+1.2mg / L 6-Ba+0.1mg / LNAA+0.2mg / LIAA+0.2mg / L KT, 30g / L sucrose, and 5.5g / L agar.

[0041] The spectral data of different LED light sources are shown in Table 1. The spectral data of the front, middle and rear sections of the lamp tube are measured for the same light source, and the average value is calculated:

[0042] Table 1 Spectral processing of different LED light sources

[0043]

[0044] After 40 days of induction, the bud length, base thickness, number of buds, number of bud nodes, growth status, degree of lignification and pseudo-leaf expansion of Neijiang Asparagus cochinchinensis were counted. The bud formation and degree of lignification of Neijiang Asparagus cochinchinensis under different light qualities are shown in Table 2.

[0045] Table 2 Effects of different light qualities on the growth and lignification degree of Neijiang Asparagus cochinchinensis seedlings

[0046]

[0047] Table 3 Effects of different light qualities on the growth and lignification degree of Asparagus cochinchinensis seedlings without clumps

[0048]

[0049] The results showed that different light qualities had a great influence on the growth indicators, lignification degree and pseudo-leaf expansion degree of Neijiang Asparagus cochinchinensis seedlings. Light quality 1 was beneficial to promote the growth of bud length of Neijiang Asparagus cochinchinensis seedlings, light quality 4 was not conducive to the proliferation and growth of base clumps and stem thickness, light quality 3 had an inhibitory effect on the growth of bud number and bud node number, light quality 4 was conducive to the growth of more buds on the same bud, but was not conducive to the differentiation of more buds at the base. Under the five light qualities, the overall growth of Neijiang Asparagus cochinchinensis seedlings was good. Under light quality 1 and light quality 4, the lignification degree of Neijiang Asparagus cochinchinensis seedlings was greatly improved compared with CK, and the pseudo-leaf was fully expanded. This type of material is suitable for rooting of Neijiang Asparagus cochinchinensis in the later stage.

[0050] Different light qualities have a great influence on the growth indexes, lignification degree and pseudo-leaf expansion degree of Neijiang Asparagus cochinchinensis seedlings without clumps. Light quality 1 can greatly promote the growth of bud length and base stem thickness of Neijiang Asparagus cochinchinensis seedlings without clumps, and promote the complete expansion of pseudo-leaf, but the overall growth state of the plant is poor. Light quality 2 can promote the complete expansion of pseudo-leaf of the plant, but it has an inhibitory effect on the overall growth of Neijiang Asparagus cochinchinensis seedlings without clumps. Light quality 3 can promote the growth of base stem thickness and bud number, and the plant growth state is good, with occasional pseudo-leaf expansion. Under light quality 4, Neijiang Asparagus cochinchinensis seedlings without clumps can promote the expansion of pseudo-leaf, inhibit the growth of bud node number, and the seedling growth state is good.

[0051] Example 2 Effects of different light quality combinations on the rooting rate of Neijiang Asparagus cochinchinensis tissue culture seedlings

[0052] The asparagus tissue culture seedlings with a high degree of pseudo-leaf differentiation in the late proliferation stage of Neijiang asparagus were inoculated into rooting medium and cultured in LED lamps with different light qualities. Commonly used white light LED lamps were used as controls to observe the effects of different LED light sources on the rooting rate and growth of Neijiang asparagus rooting seedlings (the spectral data of different light sources are shown in Table 1). The formula of the rooting medium is: 1 / 4MS (1 / 4 iron salt) + IBA 0.5mg / L + IAA 0.5mg / L + KT 0.05mg / L + CCC 4.0mg / L + banana 50g / L, sucrose 20g / L, agar 5.5g / L.

[0053] After 60 days of cultivation, the root number, root length and rooting rate were counted; the effects of different light qualities on the rooting rate of Neijiang Asparagus cochinchinensis are shown in Table 3:

[0054] Table 4 Effects of different light qualities on the rooting rate of Asparagus cochinchinensis

[0055]

[0056] As shown in Table 4, different light quality combinations have an impact on the growth and rooting rate of Neijiang Asparagus cochinchinensis. Under light quality 2, Neijiang Asparagus cochinchinensis has a better growth potential and the highest rooting rate, reaching 65%, which is much higher than the 43.3% of conventional light sources. Light quality 1 and light quality 4 are also conducive to improving the growth potential and rooting rate of Neijiang Asparagus cochinchinensis seedlings.

[0057] In summary, in the factory production of asparagus tissue culture seedlings, the use of light quality 1 in the proliferation stage and light quality 4 in the rooting stage can shorten the growth time of asparagus tissue culture seedlings to the greatest extent, thereby shortening the production cycle of asparagus tissue culture seedlings and saving production costs.

[0058] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed by the present invention should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A light quality matching method for improving the lignification degree and rooting rate of Asparagus cochinchinensis tissue culture seedlings, characterized in that: The steps include: The following light quality combinations were used during the proliferation stage of asparagus seedlings: Wavelength 380-399nm: 0.05%-0.15%; Wavelength 400-499nm: 20.5%-30.0%; Wavelength 500-599nm: 30.05%-43.5%; Wavelength 600-699nm: 29.05%-40.0%; Wavelength 700-780nm: 1.50%-2.75%; The following light quality combinations are used during the rooting stage of Asparagus: Wavelength 380-399nm: 0.04%-0.14%; Wavelength 400-499nm: 17.0%-25.0%; Wavelength 500-599nm: 35.5%-43.5%; Wavelength 600-699nm: 30.5%-41.5%; Wavelength 700-780nm: 2.4%-4.0%.

2. The light quality matching method according to claim 1, characterized in that: The steps include: The following light quality combinations were used during the proliferation stage of asparagus seedlings: Wavelength 380-399nm: 0.07%-0.13%; Wavelength 400-499nm: 22.36%-29.31%; Wavelength 500-599nm: 36.6%-43.25%; Wavelength 600-699nm: 29.36%-34.92%; Wavelength 700-780nm: 1.84%-2.02%; The following light quality combinations are used during the rooting stage of Asparagus: Wavelength 380-399nm: 0.04%-0.10%; Wavelength 400-499nm: 18.04%-24.16%; Wavelength 500-599nm: 36.06%-42.98%; Wavelength 600-699nm: 33.78%-39.8%; Wavelength is 700-780nm: 2.33%-2.69%.

3. The light quality matching method according to claim 1, characterized in that: The light intensity in both the proliferation stage and the rooting stage is 2000-4000 lx.

4. The light quality matching method according to claim 1, characterized in that: The light quantum density in the proliferation stage is 90-140umol / m 2 / s.

5. The light quality matching method according to claim 1, characterized in that: The light quantum density in the rooting stage is 40-80umol / m 2 / s.

6. The planting method according to claim 1, characterized in that: The red-to-blue light ratio during the proliferation stage of the asparagus tissue culture seedlings is 0.8-1.5, and the red light to far-red light ratio is 10.0-20.

0.

7. The planting method according to claim 1, characterized in that , the red-to-blue light ratio during the rooting stage of Asparagus cochinchinensis is 2.0-3.0, and the red light and far-red light ratio is 7.0-15.0.

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