Breeding method of cantheconidea furcellata

Abstract

The invention provides a breeding method of cantheconidea furcellata, and belongs to the technical field of insect feeding. The invention provides a breeding method of cantheconelaconus furcellatus. The breeding method comprises the step of feeding the cantheconelaconus furcellatus with frozen larvae of spodoptera litura. According to the method, fresh lapidoptera furcellata larvae are used for feeding lapidoptera furcellata, second-year-old lapidoptera furcellata nymphs can only eat second-year-old and lower-year-old lapidoptera furcellata larvae, and in the age, the lapidoptera furcellata nymphs do not die in a large number. According to the method, the survival rate of the low-age nymphs of the cantheconidea furcellatus can be remarkably increased by feeding the cantheconidea furcellatus with the frozen cantheconidea furcellatus. Meanwhile, the frozen older podoptera litura larvae are used for feeding the cantheconidea furcellata, so that the use amount of the cantheconidea furcellata is greatly reduced, mass feeding is facilitated, the labor efficiency is improved, the labor cost is saved, and large-scale feeding is facilitated. The breeding method is suitable for large-scale breeding of the cantheconidea furcellatus, so that manual rapid large-scale breeding and long-term subculture breeding of the cantheconidea furcellatus are realized, and a new way is opened up for controlling noctuidae pests which are difficult to control.

Description

Technical Field

[0001] The invention belongs to the technical field of insect breeding, and particularly relates to a breeding method for a pronghorn bug. Background Art

[0002] The fork-horned bug (Eocantheconafurcellata walff) belongs to the order Hemiptera, family Pentatomidae, subfamily Asopinae, genus Eocanthecona. It has multiple generations a year with overlapping generations, a wide distribution range, and strong activity. The forked-horned stink bug is an important polyphagous predatory natural enemy insect in agricultural and forestry production. It can prey in both nymphal and adult stages, and has a wide range of prey. It can prey on more than 30 species of insects such as Lepidoptera, Hemiptera and Coleoptera. It is particularly fond of Lepidoptera pests. It shows good searching and preying abilities on Lepidoptera larvae such as fall armyworm, fall armyworm, bean borer, diamondback moth, and cotton bollworm. At present, in the control trials of Noctuidae pests such as fall armyworm, fall armyworm, and cotton bollworm, good control effects have been achieved. Therefore, this predatory stink bug is an important biological control resource in my country, has broad application prospects, and will produce significant economic benefits and ecological value.

[0003] At present, there is a lack of large-scale breeding technology for the forked horn bug, insufficient research on artificial feed, and high cost of natural host breeding, which seriously affects the wide application of the forked horn bug in the field. However, at present, the larvae or pupae of yellow mealworms and the larvae of Spodoptera litura are mostly used as host foods for breeding forked horn bugs in China. However, the breeding of forked horn bugs by yellow mealworms as alternative hosts has the disadvantages of low nymph survival rate, long pre-oviposition period, low egg laying amount, and unsuitability for large-scale breeding of forked horn bugs. And the larvae of Spodoptera litura as a host are very susceptible to nuclear polyhedrosis virus during the breeding of Spodoptera litura, resulting in a high mortality rate of Spodoptera litura larvae, and feeding the forked horn bugs with Spodoptera litura larvae infected with nuclear polyhedrosis virus will cause a large number of nymphs and adults to die. Data show that fall armyworm larvae are excellent hosts for the forked-horned bug, but the second-instar nymphs of the forked-horned bug can only feed on the second-instar larvae of fall armyworm and below, and the third-instar larvae of fall armyworm or above will cause a large number of deaths of the second-instar forked-horned bug nymphs. As an important biological factor for controlling field armyworm pests, the cost of the forked-horned bug remains high, which greatly limits the control role of the forked-horned bug in agricultural and forestry pest control. Therefore, establishing a large-scale breeding technology system for the forked-horned bug in my country is of great significance for the long-term prevention and control of lepidopteran armyworm pests. Based on the insufficiency of existing technologies, there is currently a great demand for methods that can be used to breed the forked-horned bug on a large scale. Therefore, exploring a high-reproduction rate, low-cost large-scale breeding method for the forked-horned bug is a key issue in realizing the large-scale production of the forked-horned bug as a successful biological control technology. Summary of the invention

[0004] The invention aims to provide a breeding method for the forked horn bug, which can improve the survival rate of the forked horn bug and reduce the breeding cost.

[0005] The objective of the present invention is achieved through the following technical solutions:

[0006] The present invention provides a breeding method for a forked-horned bug, comprising:

[0007] Feed the forked horned bug with frozen fall armyworm larvae;

[0008] The fall armyworm larvae are 4-6 instar fall armyworm larvae.

[0009] Preferably, the freeze-preservation time of the fall armyworm larvae is ≤75 days; and the freeze-preservation temperature of the fall armyworm larvae is -15 to -20°C.

[0010] Preferably, the pronghorn bug starts feeding frozen fall armyworm larvae from the third day after the eggs hatch into nymphs.

[0011] Preferably, the forked horn bug eggs are fed with honey water having a honey concentration of 8% to 12% every day for the first 3 days before hatching into nymphs.

[0012] Preferably, when the 2nd instar nymphs of the forked horn bug are fed with frozen fall armyworm larvae, 80 to 120 mg are fed per 100 forked horn bugs per day; when the 3rd instar nymphs of the forked horn bug are fed with frozen fall armyworm larvae, 180 to 220 mg are fed per 100 forked horn bugs per day; when the 4th instar nymphs of the forked horn bug are fed with frozen fall armyworm larvae, 700 to 900 mg are fed per 100 forked horn bugs per day; when the 5th instar nymphs of the forked horn bug are fed with frozen fall armyworm larvae, 1400 to 1800 mg are fed per 100 forked horn bugs per day.

[0013] Preferably, when the adult S. cerasus is fed with frozen Spodoptera frugiperda larvae, each S. cerasus is fed 30 to 40 mg per day.

[0014] Preferably, the nymphs of the forked horn bug are raised in a 1-1.5L container from the 4th day after hatching from eggs to the adult; when the forked horn bug is a nymph, each container is raised with 90-100 nymphs; when the forked horn bug is an adult, each container is raised with 40-50 adults.

[0015] Preferably, a folded paper block is placed in the container; the folded paper block is folded into a fan shape from one end of the paper and then unfolded.

[0016] Preferably, the temperature during the breeding process of the forked-horned bug is 26-28° C., the humidity is 65%-75%, and the lighting conditions are light culture for 14-16 hours and dark culture for 8-10 hours.

[0017] The present invention provides application of the breeding method described in the above technical solution in long-term large-scale group rearing of Psoralea corytaenia.

[0018] Beneficial effects of the present invention:

[0019] The present invention provides a breeding method for a forked-horned bug, comprising: feeding the forked-horned bug with frozen fall armyworm larvae; the fall armyworm larvae are 4-6 instar fall armyworm larvae. When the fresh fall armyworm larvae are used to feed the forked-horned bug, the 2nd instar nymphs of the forked-horned bug can only feed on the 2nd instar or less 2nd instar larvae of the fall armyworm, and the wrong instar will cause a large number of 2nd instar nymphs to die. The present invention uses frozen fall armyworm to feed the forked-horned bug, which can significantly improve the survival rate of the low-instar nymphs of the forked-horned bug. At the same time, the frozen older fall armyworm larvae are used to feed the forked-horned bug, which greatly saves the amount of insects, is more conducive to group breeding, and at the same time improves labor efficiency, saves labor costs, and is more conducive to large-scale breeding. The breeding method is suitable for large-scale breeding of the forked-horned bug, thereby realizing artificial rapid large-scale breeding and long-term succession breeding of the forked-horned bug. The breeding method provided by the present invention has high breeding efficiency and low breeding cost, which is conducive to large-scale breeding of forked horn bugs. The sufficient supply of insect-source food (prey) has successfully solved the problems of low reproduction rate and high cost of forked horn bugs, and will open up a new way to control difficult-to-prevent pests of the Noctuidae family. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0021] Figure 1 This is a photo of a folded paper block;

[0022] Figure 2 This is a photo of frozen fall armyworm larvae. DETAILED DESCRIPTION

[0023] The invention provides a breeding method for a pronghorn bug, comprising: feeding the pronghorn bug with frozen fall armyworm larvae.

[0024] In the present invention, the fall armyworm larvae are preferably 4-6 instar fall armyworm larvae, and may also be 5-instar fall armyworm larvae. The present invention has no special limitation on the source of the fall armyworm larvae, and any conventional source in the field may be used. The present invention has no special limitation on the specifications of the fall armyworm larvae, and any conventional specifications may be used. As an optional embodiment of the present invention, the fall armyworm larvae may be artificially reared fall armyworm larvae. The present invention has no special limitation on the artificial rearing method of the fall armyworm larvae, and any conventional artificial rearing method in the field may be used. As an optional embodiment of the present invention, the fall armyworm larvae used for freezing in the present invention are the fall armyworm larvae remaining after selecting fall armyworm larvae with consistent development as breeding insects.

[0025] The present invention does not specifically limit the method of freezing fall armyworm larvae, and any conventional freezing method in the art can be used. As an optional embodiment of the present invention, the freezing temperature can be -15 to -20°C, or -15°C, -16°C, -17°C, -18°C, -19°C or -20°C. In the process of freezing the fall armyworm larvae of the present invention, the fall armyworm larvae can be fed to the forked horn bugs or continued to be frozen in the freezer after being frozen to death. As an optional embodiment of the present invention, the time of freezing the fall armyworm larvae is ≤75d, which can be 30 to 75d, or 45 to 60d. The method of freezing the fall armyworm larvae of the present invention can be: mixing the fall armyworm larvae with corn flour and freezing them. As an optional embodiment of the present invention, the particle size of the corn flour can be ≤80 mesh, or 80 mesh; the amount of corn flour added can be 1% to 3% of the weight of the fall armyworm larvae, or 2%. The invention adds corn flour when freezing fall armyworm larvae mainly to use it as a medium to prevent insect bodies from sticking together and forming a ball, thereby facilitating feeding. While maintaining the integrity of the fall armyworm larvae, the frozen fall armyworm larvae can also be easily eaten by the forked horned bug.

[0026] The present invention preferably feeds frozen fall armyworm larvae from the third day after the forked horn bugs hatch from eggs to nymphs. The present invention does not specifically limit the incubation method of the forked horn bugs, and any conventional incubation method in the art can be used. As an optional embodiment of the present invention, the incubation method of the forked horn bugs can be to collect forked horn bug egg masses for incubation. In the present invention, the incubation temperature can be 26-28°C, or 27°C. In the present invention, the incubation humidity can be 65%-80%, or 70%-75%, and the incubation humidity can be adjusted by spraying water into the paper towel padded in the incubation container. In the present invention, the incubation process can be light cultured for 14-16 hours and dark cultured for 8-10 hours, referred to as L:D=(14-16):(8-10). As an optional embodiment of the present invention, the incubation can be carried out in an incubator. As an optional embodiment of the present invention, the incubation container can be a round box; the diameter of the round box can be 9 to 11 cm, or 9, 10 or 11 cm; the height of the round box can be 4.5 to 5.5 cm, or 4.5, 5.0 or 5.5 cm. In the present invention, the number of egg masses in each round box can be 15 to 17, or 16. The present invention performs incubation until nymphs are hatched from the egg masses to obtain nymphs of the forked-horned bug.

[0027] After obtaining the nymphs of the pronghorn bug, the present invention feeds and manages the nymphs of the pronghorn bug. As an optional embodiment of the present invention, the feeding temperature of the nymphs of the pronghorn bug can be 26-28°C, or 27°C. In the present invention, the feeding humidity of the nymphs of the pronghorn bug can be 65%-80%, or 70%-75%, and the feeding humidity of the nymphs of the pronghorn bug can be adjusted by spraying water on the paper towel padded in the incubation container. In the present invention, the nymphs of the pronghorn bug can be cultured in light for 14-16h / d and in darkness for 8-10h / d. The present invention does not specifically limit the method of feeding and management, and conventional feeding and management methods in the art can be adopted.

[0028] As an optional embodiment of the present invention, the eggs of the forked horn bug hatch into nymphs for the first 2 days (referring to the first and second days after hatching into nymphs) are preferably fed with honey water every day; the mass concentration of honey in the honey water can be 8% to 12%, or 8%, 9%, 10%, 11% or 12%. In the present invention, the method of feeding honey water can be feeding with cotton balls dipped in honey water; the cotton balls can be attached to the side walls of the culture container to facilitate the feeding of the forked horn bug nymphs. As an optional embodiment of the present invention, the feeding container for the first 2 days of the eggs of the forked horn bug hatching into nymphs can be a round box; the round box can be a food-grade plastic round box; the diameter of the round box can be 9 to 11 cm, or 9, 10 or 11 cm; the height of the round box can be 4.5 to 5.0 cm, or 4.5, 4.8 or 5.0 cm. The stocking density of the forked horn bug eggs in the round box 2 days before hatching into nymphs can be 90-110 per box, or can be 90, 95, 100, 105 or 110 per box.

[0029] The present invention preferably starts feeding frozen fall armyworm larvae from the third day after the eggs of the forked horn bugs hatch into nymphs. In the present invention, when feeding the frozen fall armyworm, the frozen fall armyworm can be taken out of the freezing equipment and fed directly. In the present invention, when the eggs of the forked horn bugs hatch into nymphs and start feeding the frozen fall armyworm larvae from the third day, 80 to 120 mg of frozen fall armyworm larvae are fed per 100 forked horn bugs per day, which can be 80, 85, 90, 95, 100, 105, 110, 115 or 120 mg. As an optional embodiment of the present invention, the forked horn bugs are fed honey water from the third day after the eggs hatch into nymphs; the mass concentration of honey in the honey water can be 8% to 12%, or it can be 8%, 9%, 10%, 11% or 12%. In the present invention, the method of feeding honey water can be feeding with cotton balls dipped in honey water; the cotton balls can be attached to the side walls of the culture container to facilitate the feeding of the nymphs of the forked-horned bugs. In the present invention, the culture container for the nymphs of the forked-horned bugs to be incubated as nymphs on the 3rd day is the same as the culture container for the nymphs to be incubated as nymphs for the first 2 days; the nymphs of the forked-horned bugs are preferably added to the culture container of the forked-horned bugs on the 3rd day after the nymphs are hatched from eggs to nymphs. As an optional embodiment of the present invention, the folded paper block is folded into a fan shape from one end of the paper several times, and then unfolded. The folded paper block obtained by the folding method of the present invention can form a fan shape on the side after unfolding. The number of foldings of the present invention can be 7 to 12 times. The folded paper block can be folded with A4 paper. The present invention does not specifically limit the source of A4 paper used in the production of the folded paper block. Conventional A4 paper in the field can be used, and discarded A4 paper can also be used to reduce the breeding cost of the forked-horned bugs. The foldable paper block provided by the present invention can be unfolded when in use and folded for storage when not in use, so it is convenient to use or store. The density of the 3rd day of hatching of the eggs of the forked horn bug into nymphs is the same as the first 2 days of hatching of the eggs of the forked horn bug into nymphs.

[0030] The present invention preferably transfers the forked horn bugs to a 1-1.5L culture container on the 4th day after the eggs of the forked horn bugs hatch into nymphs. The present invention can also directly put a 1-1.5L culture container in the round box where the forked horn bugs were raised in the first 3 days on the 4th day after the eggs of the forked horn bugs hatch into nymphs. As an optional embodiment of the present invention, two folded paper blocks described in the above technical solution are added to the culture container. As an optional embodiment of the present invention, the 1-1.5L culture container can be a plastic bottle; the plastic bottle can be a food-grade plastic bottle; the diameter of the plastic bottle can be 100-120mm, or 100, 105, 110, 115 or 120mm. In the present invention, the stocking density of the culture container can be 90-100 nymphs per culture container, or 90, 95 or 100.

[0031] In the present invention, the culturing stage when the nymphs of the forked horn bugs are transferred into a 1-1.5L culture container is preferably fed with frozen fall armyworm larvae every day. As an optional embodiment of the present invention, the feeding amount of the frozen fall armyworm larvae varies with the age of the forked horn bugs. In the present invention, when the 2nd instar nymphs of the forked horn bugs are fed with frozen fall armyworm larvae, 80-120 mg is fed per 100 forked horn bugs per day, which can be 80, 85, 90, 95, 100, 105, 110, 115 or 120 mg. In the present invention, when the 3rd instar nymphs of the forked horn bugs are fed with frozen fall armyworm larvae, 180-220 mg is fed per 100 forked horn bugs per day, which can be 180, 185, 190, 195, 200, 205, 210, 215 or 220 mg. In the present invention, when the 4th instar nymphs of the forked horn bugs are fed with frozen fall armyworm larvae, 700 to 900 mg, which may be 700, 750, 800, 850 or 900 mg, are fed per 100 forked horn bugs per day. In the present invention, when the 5th instar nymphs of the forked horn bugs are fed with fall armyworm larvae, 1400 to 1800 mg, which may be 1400, 1500, 1600, 1700 or 1800 mg, are fed per 100 forked horn bugs per day.

[0032] In the present invention, if the forked horn bug develops from a nymph into an adult, the present invention continues to raise the forked horn bug adult. The present invention feeds frozen fall armyworm larvae during the forked horn bug adult raising process. As an optional embodiment of the present invention, when feeding the forked horn bug adult, each forked horn bug is fed 30 to 40 mg of frozen fall armyworm larvae per day, which can be 30, 35 or 40 mg.

[0033] As an optional embodiment of the present invention, the breeding temperature of the adult pronghorn bug can be 26-28°C, or 27°C. In the present invention, the breeding humidity of the adult pronghorn bug can be 65%-80%, or 70%-75%. In the present invention, the adult pronghorn bug can be cultured in light for 14-16h / d and in darkness for 8-10h / d.

[0034] As an optional embodiment of the present invention, the container for raising the adult pronghorn bugs may be a 1-1.5L culture container. As an optional embodiment of the present invention, the 1-1.5L culture container may be a plastic bottle; the plastic bottle may be a food-grade plastic bottle; the diameter of the plastic bottle may be 100-120 mm, or 100, 105, 110, 115 or 120 mm. As an optional embodiment of the present invention, the stocking density of the pronghorn bugs in the culture container may be 40-50 heads / bottle, or 40, 41, 42, 43, 44, 45, 46, 47, 48, 49 or 50 heads / bottle. In the present invention, the culture container preferably contains two folded paper blocks for the pronghorn bugs to climb and lay eggs.

[0035] In the process of raising adult bugs, the adult bugs preferably collect egg-laying masses every day when they start to lay eggs. In the present invention, the egg masses on the folded paper blocks are preferably cut off every day and put into a bottle to continue laying eggs. When 1 / 3 of the folded paper blocks remain, new folded paper is replaced.

[0036] In the process of raising the forked horn bug, the invention preferably cleans the residue in the insect box every day to keep the bottle clean. After the forked horn bug lays eggs, the eggs are taken out in time and placed in an incubator for hatching.

[0037] The present invention discloses a method for breeding forked horned bugs by using frozen fall armyworm larvae. During the breeding process, factors such as the nymph survival rate, nymph development period, reproduction rate, and cost-effectiveness of the forked horned bugs should be considered. The method is simple, has sufficient food sources, low cost, and convenient operation. The reproduction rate of the forked horned bugs is high, and the breeding cost is low. The survival rate, reproduction rate, and vitality of the forked horned bugs are improved, and the needs of large-scale forked horned bugs are met.

[0038] The present invention provides application of the breeding method described in the above technical solution in long-term large-scale group rearing of Psoralea corytaenia.

[0039] In the prior art, the stocking density of the forked horned bug is usually 7307 to 10961 heads / m 3 Or 4 heads / L. The present invention adopts the breeding method described in the above technical solution to raise the forked horn bug, and uses the folded paper block for the forked horn bug to climb and lay eggs, thereby increasing the stocking density of the forked horn bug. At the same time, the folded paper block can also be used as an egg-laying medium. The breeding results show that the stocking density is increased by at least 10 times. The above breeding method of the present invention achieves the effect of taking into account waste utilization and reducing breeding costs.

[0040] The breeding method of the present invention improves the survival rate of the nymphs of the forked horn bug and solves the breeding problem of high mortality of the young nymphs of the forked horn bug. The fresh and live larvae of the fall armyworm are used to feed the forked horn bug. The 2nd instar nymphs of the forked horn bug can only feed on the 2nd instar larvae of the fall armyworm and below. The wrong age will cause a large number of deaths of the 2nd instar nymphs of the forked horn bug. The present invention uses frozen fall armyworm larvae to feed the forked horn bug, which significantly improves the survival rate of the young nymphs of the forked horn bug.

[0041] The breeding method of the present invention improves the labor efficiency in the breeding process of the forked horn bug and reduces the labor cost. Feeding the forked horn bug with frozen older fall armyworm greatly saves the amount of insects, is more conducive to group breeding, and at the same time improves labor efficiency, saves labor costs, and is more conducive to large-scale breeding.

[0042] In order to further illustrate the present invention, the technical solution provided by the present invention is described in detail below in conjunction with the accompanying drawings and embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0043] The source of the fall armyworm is: it was collected from the corn fields of the Xindu base of Sichuan Academy of Agricultural Sciences in June 2019, and has been raised on a large scale in groups using artificial feed at the Institute of Plant Protection of Sichuan Agricultural College (for details of the breeding method, see Patent No.: 202211126997.6) to date.

[0044] Spodoptera litura larvae: self-feeding with artificial feed.

[0045] Mealworm pupae: Mealworm larvae were purchased from the flower and bird market and fed to the forked-horned bug after pupation.

[0046] Honey: Rapeseed honey, purchased from Jieshou Rongfei Bee Products Co., Ltd.

[0047] Folding paper block: Use discarded A4 paper, fold it into a fan shape from one end of the paper several times, and then unfold it to get a fan shape. Figure 1 shown.

[0048] In the following Examples 1 to 3 and Comparative Examples 1 to 3, 20 nymphs of the forked-horned bug were treated in each treatment, and 3 repetitions were set respectively, for a total of 360 nymphs of the forked-horned bug.

[0049] Example 1

[0050] 1. The breeding method of fall armyworm is as follows:

[0051] Special feed for the fall armyworm: composed of soybean powder, wheat flour, yeast powder, ascorbic acid, agar, glacial acetic acid, cholesterol and sorbic acid; the amount of feed each time should be enough to leave a little left over after the fall armyworm has finished eating.

[0052] 1. Egg incubation management: Place the non-woven fabric with eggs in a 22cm×13cm×6cm plastic box covered with moistened toilet paper, and then place it in an incubator for incubation. The incubation conditions are 28°C, 70% RH, and a light exposure time of L:D=16:8. When the eggs are hatched to blackheads, take them out, soak them in 5% formaldehyde solution (volume ratio) for disinfection for 15 minutes, rinse them with clean water several times, then dry them with toilet paper and continue to place them in the incubator for incubation.

[0053] 2. Larvae feeding and management: Newly hatched larvae are fed in 30.5cm×21.5cm×4cm plastic boxes. The special feed for fall armyworm larvae is cut into slices for easy feeding of larvae. Each box is covered with non-woven fabric and then overlapped into 10 boxes (i.e. 10 boxes are stacked together). They are placed on the feeding rack and then covered with the box lid. They are placed in a feeding room with a temperature of 27℃, a humidity of 70%, and a daily light condition of L:D=16h:8h. Check and add feed every day. When they are 5 years old, select larvae with relatively consistent development as seed worms. Keep about 150 larvae in each box until they become mature larvae.

[0054] The remaining 5th and 6th instar larvae are mixed with dry corn flour as a medium and then placed in a -15°C to -20°C freezer for standby use. The corn flour particle size is 80 meshes. The amount of corn flour is 2% of the insect weight. The frozen fall armyworm is used as food for predation and breeding of forked horned bugs at any time.

[0055] 3. Pupa management method: In an environment with a temperature of 27°C, a humidity of 70%, and a light condition of L:D=16:8, put the mature larvae into a plastic box with a length×width×height=37.5cm×25cm×6cm for pupation. Put sandy loam (the soil moisture content is about 10%) in the box, put about 250 mature larvae in each box, and add sliced ​​artificial feed. Check and add feed every day. No more feed will be added after burying in the soil. Remove residual feed and dead insects. After most of the mature larvae have drilled into the soil for pupation, collect the pupae in the plastic box 3 days later. Put the collected pupae into a plastic round box with a diameter of 10cm and a height of 4cm padded with moistened toilet paper, with 100 pupae in each box, and wait for them to emerge.

[0056] 4. Adult rearing method: In an environment with a temperature of 27°C, a humidity of 70%, and a daily light condition of L:D=16h:8h, the emerged adults are randomly placed in a rearing cage of length×width×height=32cm×23cm×18cm, with 300 adults placed in each cage. Non-woven fabric is placed in the cage for them to lay eggs. Cotton balls soaked in honey water with a honey mass concentration of 10% are placed in the cage, and the cage is covered with a soaked towel. The honey water cotton balls are changed once a day, and the wet towels are changed twice. After laying eggs, the egg cloth is collected once a day and stored in a 5°C refrigerator or directly placed in an incubator for incubation.

[0057] 2. A breeding method for a forked horned bug, the steps are as follows:

[0058] The 5th instar larvae of fall armyworm were frozen for 30 days as food for the predation and breeding of forked horned bugs. Figure 2 As shown, the frozen fall armyworm larvae are taken out of the freezing equipment and fed directly.

[0059] 1. Hatching management of forked horn bug eggs: collect forked horn bug egg masses, put into the round box of diameter 10cm, height 5cm, 16 eggs in each box, put paper towel on the bottom and spray water, place in the incubator of 27 ℃, humidity 70%, light condition L: D = 16h: 8h every day, spray water every day to keep moist state, until the egg masses hatch nymphs.

[0060] 2. Feeding and management of nymphs of the forked-horned bug: in an environment with a temperature of 27°C, a humidity of 70%, and a light condition of L:D=16:8.

[0061] (1) Newly hatched nymphs of the forked-horned bug were reared at a density of 100 per box. Within 2 days of hatching, the nymphs were reared in a food-grade plastic round box with a diameter of 10 cm and a height of 5 cm. Paper towels were placed at the bottom of the box, and 10% (10% means that the mass concentration of honey in the honey water is 10%) honey water cotton balls were added every day. The cotton balls were attached to the wall of the box to facilitate the forked-horned bug nymphs to feed.

[0062] (2) After the newly hatched nymphs of the forked horn bug are fed with honey water for the first 2 days, on the 3rd day, the honey water (10% honey water cotton balls) is added with frozen fall armyworm larvae, 100 heads / 80-120 mg, that is, 100 second-instar nymphs of the forked horn bug are fed with 80-120 mg of frozen fall armyworm larvae per day, and a folded A4 paper block is added. Figure 1 . On the 4th day, the nymphs of the forked horn bug were transferred to a food-grade plastic bottle with a container of 1 to 1.5 L for breeding. The diameter of the plastic bottle was 110 mm and the height was 130 mm. Two folded paper blocks were placed in the plastic bottle. The stocking density in the plastic bottle was 90 heads / bottle. The amount of frozen fall armyworm larvae fed was as follows: 100 2-year-old forked horn bugs were fed 100 mg, 100 3-year-old forked horn bugs were fed 200 mg, 100 4-year-old forked horn bugs were fed 800 mg, and 100 5-year-old forked horn bugs were fed 1600 mg.

[0063] 3. Feeding and management of adult forked horn bugs: Under the conditions of temperature 27°C, humidity 70%, and daily light conditions L:D=16h:8h, the adult stage feeding density is 45 heads / bottle, and the feeding bottle specifications are 110mm in diameter, 130mm in height, and a food-grade plastic bottle with a volume of 1-1.5L. In order to ensure the activity space of the forked horn bugs, 2 folding paper blocks are placed in the plastic bottle for the forked horn bugs to climb and lay eggs. The daily feeding amount of frozen fall armyworm larvae is 45 heads / 1600mg, that is, 35mg / head. When the adults start to lay eggs, cut off the egg blocks on the folding paper blocks every day and put them into the bottle to continue laying eggs. When 1 / 3 of the folding paper blocks remain, replace new folding paper. Collect egg blocks every day, clean the residue in the insect box, and keep the bottle clean. After the forked horn bugs lay eggs, take out the eggs in time and put them into the incubator for hatching.

[0064] Example 2

[0065] 1. The method for raising fall armyworm is the same as that in Example 1.

[0066] 2. A method for breeding a forked-horned bug, the steps are the same as those in Example 1, the only difference being:

[0067] The fifth instar larvae of the fall armyworm, which were frozen for 45 days, were used as food for the predation and breeding of the forked-horned bug.

[0068] Example 3

[0069] 1. The method for raising fall armyworm is the same as that in Example 1.

[0070] 2. A method for breeding a forked-horned bug, the steps are the same as those in Example 1, the only difference being:

[0071] The fifth instar larvae of the fall armyworm, which were frozen for 60 days, were used as food for the predation and breeding of the forked-horned bug.

[0072] Example 4

[0073] 1. The method for raising fall armyworm is the same as that in Example 1.

[0074] 2. A method for breeding a forked-horned bug, the steps are the same as those in Example 1, the only difference being:

[0075] The fifth instar larvae of the fall armyworm, which were frozen for 75 days, were used as food for the predation and breeding of the forked-horned bug.

[0076] Comparative Example 1

[0077] A method for breeding a forked horn bug, the steps are the same as those in Example 1, except that fresh larvae of fall armyworm are used to replace frozen fall armyworm. The amount of fresh fall armyworm larvae fed is as follows: 100 2-year-old forked horn bugs are fed 100 mg, 100 3-year-old forked horn bugs are fed 200 mg, 100 4-year-old forked horn bugs are fed 800 mg, and 100 5-year-old forked horn bugs are fed 1600 mg.

[0078] The age of the fall armyworm fed by each instar of the forked horn bug is consistent with the age of the forked horn bug, that is, the 2nd instar forked horn bug nymphs feed the 2nd instar fall armyworm larvae; the 3rd instar forked horn bug nymphs feed the 3rd instar fall armyworm larvae; the 4th instar forked horn bug nymphs feed the 4th instar fall armyworm larvae; the 5th instar forked horn bug nymphs feed the 5th instar fall armyworm larvae.

[0079] Clean the breeding bottles regularly to remove the carcasses and feces of fall armyworm larvae.

[0080] Comparative Example 2

[0081] A method for breeding a forked horn bug, the steps are the same as those in Example 1, except that fresh live larvae of Spodoptera litura are used to replace frozen fall armyworm, wherein 100 2nd-instar nymphs of the forked horn bug are used / 100 mg, 100 3rd-instar nymphs are used / 200 mg, 100 4th-instar nymphs are used / 800 mg, and 100 5th-instar nymphs are used / 1600 mg.

[0082] The age of the Spodoptera litura fed by each instar of the Spodoptera litura is consistent with the age of the Spodoptera litura, that is, the 2nd instar nymphs of the Spodoptera litura feed the 2nd instar larvae of the Spodoptera litura; the 3rd instar nymphs of the Spodoptera litura feed the 3rd instar larvae of the Spodoptera litura; the 4th instar nymphs of the Spodoptera litura feed the 4th instar larvae of the Spodoptera litura; and the 5th instar nymphs of the Spodoptera litura feed the 5th instar larvae of the Spodoptera litura.

[0083] Clean the breeding bottles regularly to remove the residues and feces of the Spodoptera litura larvae.

[0084] Comparative Example 3

[0085] A method for breeding a forked horn bug, the steps are the same as those in Example 1, except that: frozen fall armyworm is replaced by yellow mealworm pupae. The amount of yellow mealworm pupae fed is as follows: 100 2-year-old 100 forked horn bug nymphs are fed 100 mg, 3-year-old 100 forked horn bug nymphs are fed 200 mg, 4-year-old 100 forked horn bug nymphs are fed 800 mg, and 5-year-old 100 forked horn bug nymphs are fed 1600 mg.

[0086] Clean the breeding bottles regularly to remove mealworm pupa residues and feces.

[0087] Application Example 1

[0088] The survival number of nymphs, nymph development period, pre-oviposition period and egg-laying amount of the forked horned bugs in Examples 1 to 4 and Comparative Examples 1 to 3 were recorded. The results are shown in Table 1.

[0089] Table 1 Growth of Examples 1 to 4 and Comparative Examples 1 to 3

[0090]

[0091] From the results described in Table 1, it can be seen that the average nymph survival rate of Examples 1 to 4 of the method for breeding forked horned bugs using frozen fall armyworm of the present invention is 84.72%, the average nymph development period is 15.04 days, the average pre-oviposition period is 8.75 days, the average maximum single female single egg laying amount is 89.91 eggs, and the average total single female egg laying amount is 307.13 eggs, which are significantly higher than the breeding results of the comparative example. For comparative examples 1 to 3, the average nymph survival rate is 50.56%, the average nymph development period is 17.72 days, the average pre-oviposition period is 13.07 days, the average maximum single female egg laying amount is 62.48 eggs, and the average total single female egg laying amount is 190.07 eggs. In general, the nymph survival rate, the maximum single female egg laying amount, and the total egg laying amount of a single female in Example 1 are higher than those in Comparative Examples 1-2, and significantly higher than those in Comparative Example 3. The nymph development period and the pre-oviposition period are shorter than those in Comparative Examples 1-2, and significantly lower than those in Comparative Example 3. The data of Examples 1-4 show that the breeding of Spodoptera exigua larvae frozen for 30d to 75d can achieve similar feeding effects, with little difference.

[0092] Comparative Example 4

[0093] In the preliminary test, the second-instar nymphs of the pronghorn bug were fed with fresh 3-instar fall armyworms in the same feeding method as in Example 1. The results showed that the survival rate of the second-instar nymphs of the pronghorn bug was only 55%.

[0094] Comparative Example 5

[0095] In the preliminary test, the 3rd instar nymphs of the pronghorn bug were fed with fresh 4th instar fall armyworm, and the feeding method was the same as in Example 1. The results showed that the survival rate of the 3rd instar nymphs of the pronghorn bug was 40%.

[0096] Comparative Example 6

[0097] In the preliminary test, the third instar nymphs of the pronghorn bug were fed with fresh 5 instar fall armyworm, and the feeding method was the same as in Example 1. The results showed that the survival rate of the third instar nymphs of the pronghorn bug was 25%.

[0098] The method of breeding forked horn bugs using frozen fall armyworm larvae of the present invention adopts an independently developed artificial feed formula to raise fall armyworm larvae in large groups, uses low-temperature freezing technology to quickly lock food nutrition, has a short breeding cycle, ensures sufficient and consistent insect source supply, avoids waste of insect source food, combines scientific breeding methods, reduces labor costs, improves the egg-laying rate and fecundity of forked horn bugs, and shortens the breeding time. In exploring the breeding method of forked horn bugs, the original problem of difficult large-scale breeding of forked horn bugs with high reproduction rate has been gradually broken, thereby achieving large-scale breeding of forked horn bugs with high reproduction rate.

[0099] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A method for breeding a forked horned bug, characterized in that: include: Feed the forked horned bugs with frozen fall armyworm larvae; The fall armyworm larvae are 4-6 instar fall armyworm larvae.

2. The breeding method according to claim 1, characterized in that: The freezing storage time of the fall armyworm larvae is ≤75 days; the freezing storage temperature of the fall armyworm larvae is -20 to -15°C.

3. The breeding method according to claim 1, characterized in that: The forked horned bugs start to feed on frozen fall armyworm larvae from the 3rd day after the eggs hatch into nymphs.

4. The breeding method according to claim 1, characterized in that: The forked horn bug eggs are hatched into nymphs for 3 days before being fed with honey water with a honey mass concentration of 8% to 12% every day.

5. The breeding method according to claim 1, characterized in that: When the 2nd instar nymphs of the forked-horned bug are fed with frozen fall armyworm larvae, 80-120 mg are fed per 100 forked-horned bugs per day; when the 3rd instar nymphs of the forked-horned bug are fed with frozen fall armyworm larvae, 180-220 mg are fed per 100 forked-horned bugs per day; when the 4th instar nymphs of the forked-horned bug are fed with frozen fall armyworm larvae, 700-900 mg are fed per 100 forked-horned bugs per day; when the 5th instar nymphs of the forked-horned bug are fed with frozen fall armyworm larvae, 1400-1800 mg are fed per 100 forked-horned bugs per day.

6. The breeding method according to claim 1, characterized in that: When the adult forked-horned bugs are fed with frozen fall armyworm larvae, 30 to 40 mg is fed to each forked-horned bug per day.

7. The breeding method according to claim 1, characterized in that: The forked horn bugs are raised in a 1-1.5L container from the 4th day after the eggs are hatched into nymphs to the adult; when the forked horn bugs are nymphs, each container is raised with 90-100 nymphs; when the forked horn bugs are adults, each container is raised with 40-50 adults.

8. The breeding method according to claim 7, characterized in that: A folded paper block is placed in the container; the folded paper block is folded into a fan shape from one end of the paper and then unfolded.

9. The breeding method according to claim 1, characterized in that: The temperature during the breeding process of the forked-horned bug is 26-28°C, the humidity is 65%-75%, and the lighting conditions are 14-16h / d of light culture and 8-10h / d of dark culture.

10. Use of the breeding method according to any one of claims 1 to 9 in long-term large-scale group rearing of Pseudocerma spp.

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