Selatheconelaconus furcellatus attractant as well as preparation method and application thereof

Abstract

The invention discloses a cantheconidea furcellata attractant as well as a preparation method and application thereof, and belongs to the technical field of plant protection. Comprising 5-8 parts of cyclohexanone, 3-5 parts of fucus extract and 1-3 parts of n-eicosane. According to the cantheconidea furcellata attractant and preparation and use of the cantheconidea furcellata attractant, pests can be effectively controlled, negative effects on the environment can be reduced, natural enemy populations can be protected, and the cantheconidea furcellata attractant is a more sustainable and environment-friendly pest management strategy. Experiments prove that the attractant prepared in the embodiment not only can effectively improve the colonization rate of adult cantheconidea furcellata in tobacco plants, but also can improve the colonization rate of five-year-old nymphs of cantheconidea furcellata in tobacco plants. The method is an ideal means for pest control.

Description

Technical Field

[0001] The present invention relates to the technical field of plant protection, and particularly relates to an attractant for Eocanthecona furcellata, a preparation method thereof, and an application thereof. Background Art

[0002] Eocanthecona furcellata belongs to the insect of Asopinae, Pentatomidae, Hemiptera. It is widely distributed in regions such as Yunnan, Guizhou, Guangxi, and Fujian. This insect has multiple generations in a year. The 2nd to 5th instar nymphs and adults can all prey on pests. Its predation range is wide, including more than 40 kinds of pests such as Lepidoptera, Hymenoptera, Coleoptera, and Hemiptera. Research shows that Eocanthecona furcellata has strong predation ability on more than 20 kinds of Lepidoptera pests such as Spodoptera frugiperda, Spodoptera litura, and Spodoptera exigua, and has great application potential in biological control.

[0003] Using natural enemy insects to control pests is an important means in biological pest control. Improving the pest control effect of natural enemy insects in the field and reducing economic costs are important guarantees for the popularization and application of natural enemy insects. In practical applications, using natural enemy insects to control pests often results in unstable pest control effects in the field, thus affecting production benefits and making it difficult for natural enemies to be popularized and applied in the field. Eocanthecona furcellata is an important predatory insect in the field. It mainly preys on pests such as Spodoptera fiugiperda, Spodoptera litura, Spodoptera exigua, and Clostera fulgurita, shows strong predation ability, and has a certain interference effect on the reproduction and development of the preyed insects. It is an ideal natural enemy for controlling Lepidoptera pests in agricultural and forestry production and has good application prospects.

[0004] However, when releasing natural enemies, the problem of poor colonization of natural enemy insects is likely to occur. Therefore, it is crucial to provide an attractant that can effectively improve the colonization of Eocanthecona furcellata. Summary of the Invention

[0005] The purpose of the present invention is to provide an attractant for Eocanthecona furcellata, a preparation method thereof, and an application thereof to solve the problems existing in the above-mentioned prior art.

[0006] To achieve the above purpose, the present invention provides the following solutions:

[0007] One of the technical solutions of the present invention is a pronghorn bug attractant, which comprises 5 to 8 parts of cyclohexanone, 3 to 5 parts of fucus vesiculosus extract and 1 to 3 parts of n-eicosane in parts by weight.

[0008] The second technical solution of the present invention is a method for preparing the above-mentioned attractant forked-horned bugs, comprising the following steps: weighing each raw material according to weight, and then fully mixing until each component is evenly dispersed.

[0009] The third technical solution of the present invention is the application of the above-mentioned attractant for pronghorn bugs in pest control in tobacco plantations.

[0010] A fourth technical solution of the present invention is a method for controlling pests in a tobacco garden, which comprises spraying tobacco plants with the above-mentioned attractant forked-horned bugs to attract the forked-horned bugs for colonization.

[0011] A fifth technical solution of the present invention is the use of the above-mentioned attractant for adult pronghorn bugs in monitoring pronghorn bug populations.

[0012] Based on the above technical solution, the present invention has the following technical effects:

[0013] The present invention provides a forked horn bug attractant. The preparation and use of the forked horn bug attractant can not only effectively control pests, but also reduce the negative impact on the environment and protect the natural enemy population, which is a more sustainable and environmentally friendly pest management strategy. The present invention also confirms through experiments that the attractant prepared in the embodiment can not only effectively increase the colonization rate of forked horn bug adults on tobacco plants, but also increase the colonization rate of the fifth instar nymphs of forked horn bugs on tobacco plants. It is an ideal means for pest control. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0015] Figure 1 This is the structural diagram of the greenhouse. DETAILED DESCRIPTION

[0016] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0017] It should be understood that the terms described in the present invention are only for describing specific embodiments and are not used to limit the present invention. Additionally, for the numerical ranges in the present invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Each intermediate value within any stated value or stated range, as well as each smaller range between any other stated value or intermediate value within the stated range, is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded from the range.

[0018] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which the present invention pertains. Although the present invention only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein can also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the documents. In case of conflict with any incorporated document, the content of this specification shall prevail.

[0019] Without departing from the scope or spirit of the present invention, various improvements and changes can be made to the specific embodiments of the present invention specification, which are obvious to those skilled in the art. Other embodiments obtained from the specification of the present invention are obvious to those skilled in the art. The specification and examples of this application are merely exemplary.

[0020] Regarding the use of "comprising", "including", "having", "containing", etc. in this article, they are all open-ended terms, meaning including but not limited to.

[0021] The technical solutions described in the present invention are all conventional solutions in the art unless otherwise specified. The reagents or raw materials used are all purchased from commercial channels or have been made public unless otherwise specified.

[0022] An embodiment of the present invention provides a predator bug Eocanthecona furcellata attractant, which, by weight parts, comprises 5 - 8 parts of cyclohexanone, 3 - 5 parts of Fucus extract, and 1 - 3 parts of n - eicosane.

[0023] Cyclohexanone is an organic compound, usually used as a solvent and chemical intermediate. In the attractant, cyclohexanone may play a role due to its volatility, mimicking the preference of the predator bug Eocanthecona furcellata for certain volatile organic compounds in nature. This volatility may attract the predator bug Eocanthecona furcellata, making it mistake it for a food source or a suitable environment, thus being attracted.

[0024] n - Eicosane is a long - chain alkane, belonging to straight - chain saturated hydrocarbons. In the attractant, eicosane may, due to its specific chemical structure and volatility, mimic certain odors that the predator bug Eocanthecona furcellata may encounter in the natural environment, thus attracting them. Long - chain alkanes may be related to certain behaviors or physiological reactions of the predator bug Eocanthecona furcellata, so they are used as attractant components.

[0025] The extract of Fucus vesiculosus contains various bioactive components, such as fucoidans, mucilage (mucopolysaccharide), alginate, mannitol, β-carotene, zeaxanthin, phlorotannins, etc. These components may attract Eocanthecona furcellata due to their unique chemical properties and biological activities. Some components in the extract of Fucus vesiculosus may mimic certain chemical signals of Eocanthecona furcellata in the natural environment, or provide certain nutrients or stimuli they need, thus playing a role in attracting.

[0026] In summary, the preparation and use of this attractant for Eocanthecona furcellata can not only effectively control pests, but also reduce the negative impact on the environment and protect the natural enemy population, which is a more sustainable and environmentally friendly pest management strategy.

[0027] The embodiment of the present invention also provides a preparation method of the above-mentioned attractant for Eocanthecona furcellata, including the following steps: Weigh each raw material by weight parts, and then mix them evenly until each component is uniformly dispersed.

[0028] The embodiment of the present invention also provides the application of the above-mentioned attractant for Eocanthecona furcellata in pest control in tobacco plantations.

[0029] The embodiment of the present invention also provides a pest control method for tobacco plantations, using the above-mentioned attractant for Eocanthecona furcellata to spray tobacco plants to attract Eocanthecona furcellata to colonize.

[0030] In some specific embodiments, the pests include Spodoptera frugiperda, Spodoptera litura, Spodoptera exigua, and Poplar leaf-eating beetle.

[0031] In some specific embodiments, the Eocanthecona furcellata includes the 5th instar nymphs and adults of Eocanthecona furcellata.

[0032] The embodiment of the present invention also provides the application of the above-mentioned attractant for Eocanthecona furcellata adults in the population monitoring of Eocanthecona furcellata.

[0033] The materials used in the embodiment of the present invention are as follows:

[0034] The extract of Fucus vesiculosus is purchased from Shandong Pingju Biotechnology Co., Ltd.

[0035] Eocanthecona furcellata: Collected from Yuanjiang County, Yunnan Province. Brought back to the laboratory and reared in cages (27±1°C, RH 60% - 80%, photoperiod 14L:10D). Fed Tenebrio molitor every day, and a laboratory population has been established.

[0036] Example 1

[0037] An attractant for Eocanthecona furcellata includes 6 parts of cyclohexanone, 4 parts of the extract of Fucus vesiculosus, and 2 parts of n-eicosane. Weigh each raw material, and then mix them evenly until each component is uniformly dispersed.

[0038] Example 2

[0039] A lure for Eocanthecona furcellata, comprising 8 parts of cyclohexanone, 3 parts of Fucus extract, and 3 parts of n-eicosane. Weigh each raw material and then mix them thoroughly until each component is evenly dispersed.

[0040] Example 3

[0041] A lure for Eocanthecona furcellata, comprising 5 parts of cyclohexanone, 5 parts of Fucus extract, and 1 part of n-eicosane. Weigh each raw material and then mix them thoroughly until each component is evenly dispersed.

[0042] Comparative Example 1

[0043] A lure for Eocanthecona furcellata, comprising 6 parts of cyclohexanone and 4 parts of Fucus extract. Weigh each raw material and then mix them thoroughly until each component is evenly dispersed.

[0044] Comparative Example 2

[0045] A lure for Eocanthecona furcellata, comprising 6 parts of cyclohexanone and 2 parts of n-eicosane. Weigh each raw material and then mix them thoroughly until each component is evenly dispersed.

[0046] Comparative Example 3

[0047] A lure for Eocanthecona furcellata, comprising 4 parts of Fucus extract and 4 parts of n-eicosane. Weigh each raw material and then mix them thoroughly until each component is evenly dispersed.

[0048] Experimental Example

[0049] 1 Test site and greenhouse

[0050] The test was carried out in the tobacco field of Zhanghetun, Longchuan Town, Nanhua County, Chuxiong Prefecture. The tobacco variety was K326. During the experiment, all experimental fields were cultivated and managed with unified conventional techniques for tobacco cultivation, water, and fertilizer management, etc.

[0051] The field cage release test of Eocanthecona furcellata was carried out in the greenhouse built in the tobacco field. As Figure 1 shown, the greenhouse was 6 m long, 3 m wide, and 2.2 m high. The northeast corner of the greenhouse was used as the coordinate origin. The temperature range in the greenhouse was 17°C to 30°C, and the humidity range was 43% to 61%. The planted crop was tobacco (K326) with a height of 1.5 m. The planting density was 12 plants per row, with a total of 3 rows, a total of 36 plants, and the floor area of each plant was 0.5 m 2 .

[0052] 2 Field colonization rate of Eocanthecona furcellata

[0053] One hour before releasing Campylomma livida, spray the Campylomma livida attractants prepared in Examples 1-3 and Comparative Examples 1-3 on tobacco, and use the separate spraying of cyclohexanone and n-eicosane as the control group. Apply 5 g to each tobacco plant.

[0054] Release 25 newly emerged male and female adults of Campylomma livida each. Place the Campylomma livida in a plastic box (15 cm × 21 cm × 11 cm), and then release them at the horizontal geometric center of the greenhouse at 19:00 to allow them to disperse freely. Start the investigation after 7:00 in the morning on the second day after release. The investigation interval is 12 h, and a total of 4 investigations are carried out. Measure the spatial coordinates of each Campylomma livida. The value of the vertical coordinate Z is the vertical distance from the distribution position of Campylomma livida to the ground. Each treatment is repeated three times. Investigate its colonization rate after 3 d.

[0055] Colonization rate (%) = (number of insects at the time of investigation / number of insects at the time of release) × 100.

[0056] Table 1 Colonization rate of Campylomma livida induced by different attractants

[0057]

[0058] As can be seen from Table 1, the attractants prepared in the examples of the present invention can effectively improve the colonization rate of Campylomma livida on tobacco plants. In addition, repeat Step 2 with the Campylomma livida attractants prepared in Examples 1-3 of the present invention, and measure the colonization rate of the 5th instar nymphs of Campylomma livida on tobacco plants. The colonization rates are 50.3%, 49.7% and 47.3% respectively. It can be seen that the Campylomma livida attractants prepared in the examples of the present invention can be used as colonization attractants for the 5th instar nymphs and adults of Campylomma livida in tobacco fields.

[0059] 3 Trapping experiment

[0060] 3.1 Conduct an experiment in a tobacco field. Release 100 Campylomma livida adults at the center point of the tobacco field. The trapping device is 2 m away from the release point. The dosages of the Campylomma livida adult attractant prepared in Example 1 are 0, 5, 10, 15, 20, 25 mg respectively. Record the trapped insect quantity regularly on the 2nd day after release. The investigation time is 7 d, and repeat three times. Determine the optimal dosage of the adult attractant according to the test results.

[0061] 3.2 Conduct an experiment in a tobacco field. Release 100 Campylomma livida adults at the center point of the tobacco field. The hanging heights of the trapping device are set to 0, 0.3, 0.6, 0.9, 1.2 m. The dosage of the attractant is the dosage with the best trapping effect screened in the test of 3.1. Record the trapped insect quantity regularly on the 2nd day after release. The investigation time is 7 d, and repeat three times. Empty the Campylomma livida in the trap during each investigation.

[0062] 3.3 Conduct experiments in the tobacco field. Release 100 adult Eocanthecona furcellata at the center point of the tobacco field. Set the spacing of the trapping devices at 1, 2, 3, 5, and 7 m away from the trapping devices. The usage amount of the pheromone of the trapping device is the optimal amount screened in 3.1 experiments. Each trapping device is more than 20 m apart to avoid mutual influence caused by the too-close placement of the trapping devices. Regularly record the trapped insect quantity on the 2nd day after release. The investigation time is 7 days, and repeat three times. Determine the optimal hanging spacing according to the test results.

[0063] The results show that when the distance between the trap and the release point is 2 m and the usage amount of the attractant for adult Eocanthecona furcellata is 10 mg, the trapped quantity is the largest, and the trapping rate is 33%, which is significantly different from the lures with other usage amounts of the attractant.

[0064] The optimal attracting range of the attractant for adult Eocanthecona furcellata is 1 - 2 m, and the trapping rates are 35.67% and 32.33% respectively, which are significantly different from the attracting effects at other distances. The attracting effect on adults weakens with the increase of the placement distance of the lure, and the trapping rate also decreases. When the lure is placed at 7 m, the trapping rate of adult Eocanthecona furcellata is 7.67%.

[0065] There are significant differences in the attracting effect of the lure core of the attractant for adult Eocanthecona furcellata at different placement heights, indicating that the placement height of the lure core has an obvious impact on its attracting effect. When the lure core is placed at a height of 30 cm above the ground, the trapped quantity of adult Eocanthecona furcellata is significantly more than that at other heights, and its trapping rate is 21.33%. When the lure core is placed at heights of 60 cm and 90 cm above the ground, and when the lure core is placed at a height of 120 cm above the ground, the trapping rate is the lowest.

[0066] Obviously, the above-mentioned embodiments of the present invention are only examples for clearly explaining the present invention, rather than limiting the implementation manners of the present invention. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation manners here. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present invention shall be included in the protection scope of the claims of the present invention.

Claims

1. A pronghorn bug attractant, characterized in that: Calculated by weight, the invention comprises 5-8 parts of cyclohexanone, 3-5 parts of fucus vesiculosus extract and 1-3 parts of n-eicosane.

2. The method for preparing the attractant for the forked horned bug according to claim 1, characterized in that: The method comprises the following steps: weighing each raw material according to weight, and then fully mixing them until each component is evenly dispersed.

3. The application of the attractant for Psoralea corylifolia as claimed in claim 1 in pest control in tobacco gardens.

4. A method for controlling pests in a tobacco garden, characterized in that: The forked horn bug attractant according to claim 1 is used to spray tobacco plants to attract the forked horn bug to colonize.

5. The method for controlling pests in tobacco garden according to claim 4, characterized in that: The pests include fall armyworm, fall armyworm, beet armyworm and poplar leaf-feeding beetle.

6. The method for controlling pests in tobacco garden according to claim 4, characterized in that: The forked horn bug includes the fifth instar nymph and adult of the forked horn bug.

7. Use of the attractant for adults of the pronghorn bug as claimed in claim 1 in monitoring the pronghorn bug population.

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