A method for shortening the flowering period of non-vernalized brassica napus seeds by treatment with gibberellins

Abstract

The present application belongs to the technical field of plant cultivation, and particularly relates to a method for shortening flowering period of non-vernalized Brassica napus seed by using gibberellin treatment. The present application discloses a method for promoting vernalization of winter rape by using gibberellin, which comprises the following steps: (1) subjecting Brassica napus seed SC4 to constant-temperature light-proof germination treatment in a solution containing gibberellin to obtain white Brassica napus seed SC4; and (2) culturing the white Brassica napus seed SC4 under long-day conditions. Under the long-day light conditions, the flowering period of the non-vernalized Brassica napus seed SC4 is extremely significantly shortened and significantly shortened under the treatment of 0.001 mol / L and 0.002 mol / L gibberellin concentration, respectively.

Description

Technical Field

[0001] This invention belongs to the field of plant cultivation technology, specifically relating to a method for shortening the flowering period of unvernalized rapeseed seeds using gibberellin. Background Technology

[0002] Rapeseed is my country's most important oilseed crop, providing more than 55% of the raw materials for domestically produced edible vegetable oil. Developing rapeseed production is of great strategic significance for safeguarding the country's edible oil supply security.

[0003] Brassica napus is the most important cultivated type of rapeseed in my country, accounting for more than 90% of the rapeseed planting area. Originating in Europe, this type of rapeseed has been successfully domesticated from a "winter-type" to a "semi-winter-type" through decades of modern breeding and improvement. It has become the main type of rapeseed currently being promoted for cultivation, with a growth period of about 210 days (Hu JH et al., 2022). However, it still needs to undergo a relatively long period of low-temperature vernalization before flowering and fruiting.

[0004] The development of modern rapeseed seed industry requires accelerating the generation and purification process to improve breeding efficiency. The speed of this process is mainly controlled by the growth period. During the summer breeding of rapeseed in northern regions or indoors, there may be phenomena such as delayed flowering, delayed flowering of materials with strong winter hardiness, or inability to flower through vernalization, which affect the generation breeding process. It is necessary to develop simple and easy-to-implement methods to promote rapeseed vernalization and improve the efficiency of generation propagation and breeding.

[0005] Gibberellins are a class of tetracyclic diterpenoid plant growth factors that participate in and influence multiple aspects of plant growth and development, including seed germination, stem and petiole elongation, flower induction, and floral organ development. Gibberellin signaling promotes plant growth by initiating the degradation of the DELLA protein, which in turn inhibits the expression of a series of flower bud induction genes, ultimately resulting in gibberellin signaling promoting flowering.

[0006] Existing technologies include studies on using gibberellin at certain concentrations to spray flower buds to promote vernalization in plants, but these methods are not very feasible in rapeseed field production. This invention involves treating rapeseed with different concentrations of gibberellin during the seed germination stage. Under long-day conditions, this can promote earlier vernalization and flowering in rapeseed, providing a solution to address the problem of flowering sterility in rapeseed hybrid seed production and improving the efficiency of multigeneration seed production. Summary of the Invention

[0007] In order to promote early flowering of rapeseed under long-day conditions, the present invention provides the following technical solution:

[0008] This invention provides a method for shortening the flowering period of unvernalized rapeseed seeds using gibberellin, the method comprising the following steps:

[0009] (1) Brassica napus seed SC4 is treated with gibberellin solution for constant temperature and light-proof germination to obtain Brassica napus seed SC4 with white sprouts;

[0010] (2) The Brassica napus seed SC4 with white sprouts is cultured under long-day conditions.

[0011] Preferably, the concentration of gibberellin in step (1) is 0.001 mol to 0.002 mol / L.

[0012] Preferably, the accession number of Brassica napus seed SC4 in step (1) is CCTCC NO: P202413.

[0013] Preferably, the long-day conditions in step (2) are long-day culture conditions of 18-22°C in a light culture room, 18h / 6h.

[0014] Advantages of the present application:

[0015] Under the light conditions of long-day, the flowering period of Brassica napus seed SC4 without vernalization can be significantly or extremely significantly shortened under the treatment of gibberellin concentration of 0.001 mol / L to 0.002 mol / L, while the effect of gibberellin concentration on shortening the flowering period of the material is not significant after vernalization. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 Comparison of flowering period of Brassica napus SC4 under different gibberellin treatment concentrations (left: bud stage; right: flowering period).

[0017] Figure 2 Comparison of flowering period data of Brassica napus material SC4 under different gibberellin concentrations under different long-day low-temperature treatments. DETAILED DESCRIPTION

[0018] In order to make the purpose, technical scheme and advantages of the present application clearer, the technical scheme in the embodiments of the present application will be described clearly and completely below in combination with the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0019] MATERIALS AND METHODS

[0020] The Brassica napus (SC4) seeds were selected for the experiment.

[0021] The Brassica napus seed SC4 was deposited in the China Center for Type Culture Collection on April 25, 2024, with the deposit number CCTCC NO: P202413, and the deposit location Wuhan, China.

[0022] The two materials were placed in solutions containing different concentrations of gibberellin A3 (GA3) (BioBomei, CG3131, C 19 H 22 O6) for germination period treatment.

[0023] The Brassica napus seed SC4 was placed in a culture dish with a diameter of 10 cm, lined with four layers of filter paper, and 10 ml of GA3 solution was poured into each dish, with concentrations of 0, 0.001, 0.002, and 0.005 mol / L. Twenty healthy and full seeds were placed in each dish, with three dishes for each concentration as three replicates. The seeds were germinated in a constant temperature incubator at 22°C in the dark for 24 hours until they were white. Then, they were transferred to blank water-absorbing filter paper and treated at 4°C in a constant temperature incubator for 0 days, 3 days, and 6 days, respectively. Then, they were moved to a seedling tray and cultured in a photoperiodic culture room at 22°C under long-day conditions of 18h / 6h. The flowering time of the materials under different treatment conditions and gradients was recorded.

[0024] Gibberellin solution preparation method: 3.464g was dissolved in 10ml 75% alcohol, 1ml was taken and diluted to 100ml to prepare a 0.001mol / L solution; 2ml was taken and diluted to 100ml to prepare a 0.002mol / L solution; 5ml was taken and diluted to 100ml to prepare a 0.005mol / L solution.

[0025] Experimental results

[0026] Under long-day light conditions, Brassica napus seeds SC4 without vernalization were significantly and significantly shortened in flowering period at gibberellin concentrations of 0.001mol / L and 0.002mol / L, respectively, while gibberellin at each concentration had no significant effect on shortening the flowering period of materials after vernalization.

[0027] Conclusion:

[0028] (1) The application scenario of using 0.001mol / L gibberellin to promote flowering in the germination period is not suitable for spring materials with low vernalization requirements, but it has a wide application scenario for winter materials with high vernalization requirements.

[0029] (2) Through repeated experiments, it was found that the concentration of 0.01 mol / L gibberellin had a toxic effect on seed germination, and 0.005 mol / L had an effect of prolonging the flowering period. It is possible that a small amount of toxic effect in the early stage delays the growth and flowering of the material, and how the effect of lower concentration needs more verification.

[0030] Specific data analysis results:

[0031]

Claims

1. A method for shortening the flowering period of non-vernalized Brassica napus seeds by treatment with gibberellins, characterized in that, The method comprises the following steps: (1) Unverified rapeseed seeds ( Brassica napus (seed)SC4 was subjected to isothermal germination in a 0.001 mol / L gibberellin solution to obtain unvernalized Brassica napus seeds with white sprouts. Brassica napus seed) SC4; the unvernalized Brassica napus seed ( Brassica napus seed The accession number for SC4 is CCTCC NO: P202413; (2) The unvernalized rapeseed seeds that have just emerged from the soil ( Brassica napus Seed SC4 was cultured under long-day conditions.

2. The method of claim 1, wherein, In step (2), the long-day condition is a long-day culture condition of 18-22 DEG C light culture room, 18h / 6h.

Images