Chinese herbal medicine feed additive for laying hens

Abstract

The invention discloses a Chinese herbal medicine feed additive for laying hens, relates to the technical field of feed additives, and is technically characterized in that the feed additive is prepared from the following raw materials in parts by weight: 2-4 parts of scutellaria baicalensis, 4-6 parts of poria cocos, 4-6 parts of codonopsis pilosula, 2-4 parts of cinnamon, 4-8 parts of angelica sinensis, 4-8 parts of honey-fried licorice root and 8-10 parts of gardenia jasminoides. The Chinese herbal medicine feed additive for laying hens provided by the invention effectively improves the production performance and immunity of laying hens. The laying rate of a test group using the additive reaches 91.24%, the average egg weight is increased to 56.72 g, meanwhile, the feed-egg ratio is improved, the soft and broken egg rate is reduced, and it is indicated that the feed utilization efficiency is improved, and the egg quality is improved. In addition, liver function indexes ALT and AST are remarkably reduced, inflammatory indexes IL-1beta and IFN-gamma are reduced, and IL-4 is increased, so that the immunity is enhanced. And the content of unsaturated fatty acid in the eggs is remarkably increased, so that the nutritional value of the eggs is improved. On the whole, the additive has positive influence on health and production performance of laying hens, and has good application potential.

Description

Technical Field

[0001] The invention relates to the technical field of feed additives, and in particular to a Chinese herbal medicine feed additive for laying hens. Background Art

[0002] The harm of excessive use of antibiotic additives As people's living standards continue to improve, more and more attention has been paid to the safety of feed additives, especially the addition of antibiotics. Through investigation, it was found that the use of antibiotics can make bacteria resistant, and the antibiotics remaining in the body will also affect the immune effect of animals and people. Excessive use of antibiotics can easily destroy the balance of microecology in the intestines of animals. There are many microorganisms in the intestines of animals that are sensitive to antibiotics. Excessive use of antibiotics will reduce these microorganisms, so that microorganisms with poor sensitivity can obtain a large number of reproduction space, thereby destroying the balance of internal microecology. In addition, after the microecological balance is destroyed, microorganisms outside the body will also invade, eventually leading to cross infection. However, from the perspective of food safety and public health safety, drugs are easy to remain, accumulate and transfer in animals, thereby threatening human health and the ecological environment. Therefore, in the feed industry, how to develop green and environmentally friendly Chinese medicine feed additives has become a research hotspot.

[0003] Research has found that Chinese herbal medicine added to feed not only has obvious biological activity, but also has the advantages of no pollution, no residue, no drug resistance, etc. It can be used as a green feed additive in animal breeding. Many Chinese herbal medicines have pharmacological activities such as antioxidant, immune-enhancing, anti-inflammatory, and antibacterial effects. At the same time, they have the advantages of low toxicity, little residue, and enhanced immunity. They have broad application prospects in animal husbandry. Summary of the invention

[0004] The purpose of the present invention is to solve the above problems and provide a laying hen Chinese herbal feed additive, which has the effect of improving the production performance of laying hens, enhancing the immunity of laying hens and improving the intestinal structure and environment of laying hens.

[0005] In order to achieve the above object, the technical solution of the present invention is as follows: a Chinese herbal medicine feed additive for laying hens, the feed additive is made of the following raw materials in parts by weight:

[0006] 2-4 parts of Scutellaria baicalensis, 4-6 parts of Poria cocos, 4-6 parts of Codonopsis pilosula, 2-4 parts of cinnamon bark, 4-8 parts of Angelica sinensis, 4-8 parts of roasted Licorice root, and 8-10 parts of Gardenia jasminoides.

[0007] Preferably, the feed additive is made from the following raw materials in parts by weight:

[0008] 3 parts of Scutellaria baicalensis, 5 parts of Poria cocos, 5 parts of Codonopsis pilosula, 3 parts of Cinnamon bark, 6 parts of Angelica sinensis, 6 parts of Roasted Licorice root, and 9 parts of Gardenia jasminoides.

[0009] Preferably, the particle size of various raw materials of the feed additive is 40 mesh.

[0010] Preferably, the feed additive is 1.5% of the total amount of the herbal feed for laying hens.

[0011] Compared with the prior art, this solution has the following beneficial effects:

[0012] It effectively improved the production performance of laying hens: the egg production rate of the test group using additives reached 91.24%, the average egg weight increased to 56.72 grams, at the same time, the feed-egg ratio improved, and the soft egg breakage rate decreased, which also indirectly indicated that the feed utilization efficiency was improved and the egg quality was improved.

[0013] It effectively improved the immunity of laying hens: the liver function indicators ALT and AST were significantly reduced, the inflammatory indicators IL-1β and IFN-γ decreased, and IL-4 increased, indicating enhanced immunity.

[0014] The nutritional value of eggs has been improved: the antioxidant capacity has been significantly improved, and the content of unsaturated fatty acids in eggs has been significantly increased. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 Schematic diagram of the effect of feed additives on the intestine in an embodiment of the present invention. DETAILED DESCRIPTION

[0016] In order to enable those skilled in the art to better understand the solution of the present invention, the technical solution of the present invention will be further described in detail below in conjunction with the embodiments of the present invention and the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0017] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present invention can be combined with each other. The present invention will be described in detail below in conjunction with the embodiments.

[0018] Example:

[0019] A Chinese herbal medicine feed additive for laying hens is prepared from the following raw materials in parts by weight: 3 parts of scutellaria baicalensis, 5 parts of poria cocos, 5 parts of codonopsis pilosula, 3 parts of cinnamon bark, 6 parts of angelica sinensis, 6 parts of roasted liquorice root and 9 parts of gardenia fruit.

[0020] Preparation method of feed additive:

[0021] According to the above ratio, the amount of each medicinal material is accurately weighed, and each medicinal material is crushed separately to ensure that the particles are uniform and the particle size is suitable for subsequent screening.

[0022] Mixing: Mix the crushed medicinal materials in a mixer to ensure uniform mixing.

[0023] Sieving: Pass the mixed medicinal materials through a 40-mesh sieve to remove larger particles and ensure the fineness and uniformity of the finished product.

[0024] Packaging: Bag the sieved powder and use a sealed bag to keep it dry and prevent moisture.

[0025] Storage: Store packaged feed additives in a cool, dry place to maintain their effectiveness.

[0026] How to use feed additives:

[0027] Add Chinese herbal feed additives at 1.5% of the basic diet of laying hens.

[0028] The following experiments are conducted to demonstrate the actual effect of the Chinese herbal medicine feed additive for laying hens provided by the present invention.

[0029] 1) Test materials

[0030] 360 40-week-old SPF laying hens were randomly divided into 2 groups, with 6 replicates in each group and 30 hens in each replicate. The control group was fed with a basic diet, and the experimental group was fed with a diet supplemented with 1.5% Chinese herbal medicine. The pre-test period was 7 days, and the formal test period was 28 days.

[0031] The composition of the basal feed for laying hens in this embodiment is shown in Table 1.

[0032] Table 1 Laying hens Basic feed composition ratio

[0033] corn 61.4％ Wheat bran 15％ Soybean meal 21.0％ Calcium Hydrogen Phosphate 1.2％ Stone powder 1.1％ salt 0.3％ total 100％

[0034] 2) Feeding management and feeding test

[0035] The chickens are raised in three-layer three-dimensional cages, with 6 chickens in each cage. The chicken house has automatic temperature control, the lighting time is 16h / d, and they are fed three times a day in the morning, noon and evening. The laying hens are allowed to drink water freely, and they are immunized, dewormed and disinfected normally.

[0036] 3) Effect performance test

[0037] Production performance measurement indicators

[0038] After the start of the production performance test, the number of live laying hens, the number of eggs laid, the average egg weight (20 eggs were randomly weighed in each replicate every day), the number of soft-broken eggs, and the number of dead and eliminated chickens were recorded every day. The feed consumption of each replicate of the test was recorded, and the egg production rate, average egg weight, soft-broken egg rate, and feed-egg ratio were calculated. Egg production rate = number of eggs laid / number of live laying hens in the test × 100%, (1) average egg weight = total qualified egg weight / number of qualified eggs, (2) soft-broken egg rate = number of soft-broken eggs / total number of eggs laid × 100%, (3) feed-egg ratio = average daily feed intake / average daily egg weight.

[0039] Production performance measurement method

[0040] During the experimental period, eggs were collected at 10:00 a.m. and 5:00 p.m. every day, and the egg weight, number of eggs laid and number of preservative eggs in each group were recorded. The egg laying rate was calculated: egg laying rate = number of eggs laid / number of experimental chickens × 100%.

[0041] Liver function index

[0042] Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were determined using a fully automatic biochemical analyzer (Beijing Beijian Xinchuangyuan Biotechnology Co., Ltd.).

[0043] Inflammatory markers

[0044] Enzyme-linked immunosorbent assay (Beijing Zhongshang Biotech Co., Ltd.) was used to measure the levels of interleukin-1β (IL-1β), interleukin-4 (IL-4), and interferon-γ (IFN-γ).

[0045] Antioxidant index

[0046] The malondialdehyde (MDA) content, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and total antioxidant capacity (T-AOC) were determined by colorimetry (Nanjing Jiancheng Bioengineering Institute).

[0047] Determination of fatty acid content in eggs

[0048] The fatty acid content in eggs was determined according to the National Food Safety Standard - Determination of Fatty Acids in Foods (GB5009.168-2016).

[0049] Determination of blood biochemical indicators

[0050] After feeding with the Chinese herbal feed additive for 15 days, that is, at the end of the experimental period, blood was collected from each group of chickens through the wing vein, 2 mL / bird, and the blood biochemical indicators of each group of chickens were measured using a fully automatic multifunctional biochemical analyzer.

[0051] Intestinal testing

[0052] The intestinal tissues of laying hens were fixed in 10% formaldehyde solution, embedded in paraffin, sliced ​​and stained with hematoxylin-eosin (HE). The slices were examined under an optical microscope.

[0053] 4) Test results

[0054] 1. Chinese herbal medicine additive compound feed significantly improves egg production performance

[0055] The Chinese herbal medicine added feed provided by the present invention can effectively improve the egg production rate and egg weight; the daily feed intake, feed-to-egg ratio, and soft-break egg rate are all reduced (Table 2); indicating that the Chinese herbal medicine added feed has a good effect.

[0056] Table 2 Effects of Chinese herbal medicine added to feed on laying hen production performance

[0057] project Basic Group Add Group Egg production rate / % 90.37±0.72 91.24±0.35 Average daily feed intake / [g / (bird·d)] 107.25±1.04 106.42±0.83 Average egg weight / (g / egg) 55.83±0.15 56.72±0.31 Feed to Egg Ratio 2.19±0.03 2.13±0.03 Soft broken egg rate / % 1.01±0.01 0.93±0.01

[0058] 2. Chinese herbal medicine additive compound feed can significantly improve the immunity of laying hens

[0059] Compared with the basic group, the serum ALT and AST activities and IL-1β and IFN-γ levels of laying hens in the Chinese herbal medicine feed group were significantly reduced, and the serum IL-4 was significantly increased (Table 3, Table 4, Table 5). This shows that Chinese herbal medicine feed can significantly improve the immunity of laying hens.

[0060] Table 3 Liver function indexes

[0061] project Basic Group Add Group ALT / (U / L) 1.29±0.19 1.23±0.13 AST / (U / L) 418.79±40.72 410.52±22.83

[0062] Table 4 Inflammatory indexes

[0063] project Basic Group Add Group IL-1β / (ng / L) 12.82±0.53 7.99±0.23 IL-4 / (ng / L) 9.28±0.34 12.03±0.63 IFN-γ / (ng / L) 8.14±0.62 6.57±0.61

[0064] Table 5 Antioxidant index

[0065] project Basic Group Add Group MDA / (μmol / L) 4.56±0.25 3.73±0.13 SOD / (U / mL) 99.95±1.89 104.26±1.62 GSH-Px / (U / mL) 1052.92±11.72 1158.53±5.84 T-AOC / (U / mL) 8.09±1.02 9.93±0.93

[0066] 3. Adding Chinese herbal medicine to feed can increase the unsaturated fatty acids in eggs

[0067] The contents of palmitic acid, linoleic acid, α-linolenic acid, behenic acid and DHA were significantly increased, indicating that the compound feed with Chinese herbal additives can significantly increase the content of unsaturated fatty acids (Table 6).

[0068] Table 6 Effects on fatty acid content in eggs

[0069]

[0070] 4Can improve key blood biochemical indicators

[0071] In this experiment, albumin, total protein and globulin were higher than those in the control group, which is closely related to the fact that Chinese herbal medicine added to feed can improve immunity. Aspartate transferase, creatine kinase, urea nitrogen and triglycerides were significantly lower than those in the basic group (Table 7), which shows that Chinese herbal medicine can reduce liver damage, fatty liver and kidney damage.

[0072] Table 7 Blood biochemical indexes

[0073] Biochemical indicators Basic Group Add Group albumin 12.54±2.41 14.95±1.25 Total Protein 43.36±0.43 45.53±0.68 globulin 25.38±0.39 26.12±0.95 Aspartate transferase 184.34±3.25 155.29±3.21 Creatine kinase 715.78±27.17 622.67±5.38 Urea nitrogen 1.33±0.04 0.89±0.15 Triglycerides 3.49±1.47 2.07±0.38

[0074] 5. Can improve intestinal structure

[0075] like Figure 1 As shown, the Chinese herbal medicine additive compound feed provided by the present invention can significantly increase the height of duodenal villi and crypt depth, significantly reduce the crypt depth of jejunum, and significantly increase the height of ileal villi.

[0076] The above specific embodiments are merely explanations of the present invention and are not limitations of the present invention. After reading this specification, those skilled in the art may make modifications to the embodiments without any creative contribution as needed. However, such modifications are protected by the patent law as long as they are within the scope of the claims of the present invention.

Claims

1. A Chinese herbal medicine feed additive for laying hens, characterized in that: The feed additive is made from the following raw materials in parts by weight: 2-4 parts of Scutellaria baicalensis, 4-6 parts of Poria cocos, 4-6 parts of Codonopsis pilosula, 2-4 parts of cinnamon bark, 4-8 parts of Angelica sinensis, 4-8 parts of roasted Licorice root, and 8-10 parts of Gardenia jasminoides.

2. The Chinese herbal medicine feed additive for laying hens according to claim 1, characterized in that: The feed additive is made from the following raw materials in parts by weight: 3 parts of Scutellaria baicalensis, 5 parts of Poria cocos, 5 parts of Codonopsis pilosula, 3 parts of Cinnamon bark, 6 parts of Angelica sinensis, 6 parts of Roasted Licorice root, and 9 parts of Gardenia jasminoides.

3. The Chinese herbal medicine feed additive for laying hens according to claim 1, characterized in that: The particle size of various raw materials of the feed additive is 40 meshes.

4. The Chinese herbal medicine feed additive for laying hens according to claim 1, characterized in that: The amount of the feed additive is 1.5% of the total amount of the herbal feed for laying hens.

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