Method for improving preparation efficiency of starch ester compound

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By using the enzyme method of combining GDSL lipase OsGLIP11 with starch and phospholipids, the problem of low preparation efficiency of existing starch-bearing complexes is solved, and efficient preparation of resistant starch is achieved, which is suitable for high-resistant starch rice for diabetic patients.

CN119699596APending Publication Date: 2025-03-28CHINA NAT RICE RES INST

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Patent Information

Application Number: CN202510035430.5
Authority / Receiving Office: CN · China
Patent Type: Applications(China)
Current Assignee / Owner
Filing Date: 2025-01-09
Publication Date: 2025-03-28

Application Information

Patent Timeline

09 Jan 2025

Application

CN202510035430.5

28 Mar 2025

Publication

CN119699596A

IPC: A23L33/25; A23L29/00; C12N9/20; C12N15/82; C12N15/55; A01H5/00; A01H6/46

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Application Domain

Hydrolases Fermentation

Technology Topics

Nucleotide Acyl group

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AI Technical Summary

Technical Problem

The existing starch composite preparation methods are relatively low in efficiency and are difficult to meet the requirements of simplicity, efficiency and environmental protection.

Method used

The starch-lipid complex was prepared by using GDSL lipase OsGLIP11 as an enzyme by extracting OsGLIP11 protein from rice and combining with starch and phospholipids.

Benefits of technology

It significantly improves the preparation efficiency of starch-flavored complexes, increases the content of resistant starch in rice, improves its digestive characteristics, and is suitable for high-resistant starch rice for diabetics.

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Abstract

The invention belongs to the technical field of food engineering, discloses a method for improving the preparation efficiency of a starch ester compound, and particularly relates to application of GSDL lipase OsGLIP11 in preparation of the starch ester compound. The invention discloses an amino acid sequence of OsGLIP11, which is shown in an ST.26 standard sequence nucleotide or amino acid sequence table. The starch is rice starch, and the lipid is 1, 2-dioleoyl-sn-glycerol-3-phosphorylethanolamine (DOPE) and 1, 2-distearoyl-sn-propyltriyl-3-phosphatidyl ethanolamine (DSPE), and the lipid is one or more than one or more than one or more than one or more than one or more than one or more than one or more than one or more than one or more than one or more than one or more than one or more than one or more than one or more than one or By applying more active GDSL lipase OsGLIP11, the content of a starch ester compound formed by rice starch, DOPE and DSPE can be remarkably increased. According to the method disclosed by the invention, the GDSL lipase OsGLIP11 is added, so that the starch interacts with the exogenous lipid to generate the starch lipid compound more easily. The method can provide a theoretical reference for increasing the content of the resistant starch in the rice and further improving the digestion characteristic of the rice, and the high-resistant starch rice suitable for diabetics to eat is created.

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