Preparation method of tobacco essential oil and application thereof

Abstract

The application discloses a preparation method of tobacco essential oil and application thereof, and belongs to the technical field of tobacco processing. The preparation method comprises the following steps: (1) drying tobacco leaves to less than 5% of moisture, and crushing the tobacco leaves into tobacco powder; (2) adding the tobacco powder, an extraction solvent and a ferroferric oxide nanoparticle composite material (FG) wrapped by graphene nanocoil into an extraction tank for extraction, and cooling and filtering the extraction tank to obtain a crude extract; wherein the mass ratio of the tobacco powder and the extraction solvent is 1: (8-12), and the addition amount of the FG is 1 ‰ of the total mass; (3) transferring the crude extract into a concentration tank, and performing vacuum concentration to remove the organic solvent and water, so as to obtain an extract; and (4) using a membrane separation technology to obtain the tobacco essential oil from the extract. The tobacco essential oil can be well coordinated with cigarette smoke, can obviously improve smoke quality, can soften cigarette smoke, can enrich aroma, can improve the sweet and smooth feeling and delicacy of the cigarette, can reduce irritation, and has the potential for industrial production.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of tobacco essential oil, in particular to a preparation method of tobacco essential oil and application thereof. BACKGROUND

[0002] Tobacco essential oil is a kind of compound with tobacco characteristic odor extracted from tobacco, which has volatility, and main components are terpenes, volatile acids, aromatic rings and the like, and can enrich the aroma of cigarettes and improve the sensory quality of cigarettes when added to cigarettes.

[0003] At present, the extraction methods of tobacco essential oil mainly include steam distillation method, organic solvent extraction method and supercritical fluid extraction method. The steam distillation method has simple equipment and operation, but the sample is azeotropic with water during extraction, which is not conducive to the extraction of heat-sensitive substances. The supercritical fluid extraction is a new technology developed in recent years, but the extraction rate is low and the extraction of flavor substances is insufficient. Dynamic reciprocating magnetic field assisted extraction is a combination of solvent extraction and magnetic effect, which can effectively improve the magnetic effect of the solvent system under the action of the magnetic field, speed up the solvent extraction and improve the extraction efficiency. The present application develops tobacco essential oil by means of external magnetic field to strengthen the separation process, which has the advantages of high efficiency, easy operation, green safety, industrial production and the like. SUMMARY

[0004] The technical problem to be solved by the present application is to provide a preparation method of tobacco essential oil and application thereof, aiming at solving the problems of low efficiency, high cost and unsuitability for heat-sensitive substances of traditional tobacco essential oil extraction method.

[0005] The technical problem to be solved by the present application is solved by the following technical scheme:

[0006] A preparation method of tobacco essential oil, comprising the following steps:

[0007] (1) drying the tobacco leaves in an oven to less than 8% moisture, and crushing into tobacco powder;

[0008] (2) adding the tobacco powder, extraction solvent and graphene nanoroll wrapped ferroferric oxide nanoparticle composite material (FG) into an extraction tank for extraction, and cooling and filtering the extraction to obtain a crude extract; wherein the mass ratio of the tobacco powder to the extraction solvent is 1:(8-12), and the addition amount of FG is 1 ‰ of the total mass;

[0009] (3) transferring the crude extract to a concentration tank, and concentrating under reduced pressure to remove the organic solvent and water, thereby obtaining an extract;

[0010] (4) obtaining tobacco essential oil from the extract by using membrane separation technology.

[0011] Preferably, in the technical scheme, step (1) is specifically: the tobacco leaves are kept in an oven at 40-50 DEG C for 12-24h, and then crushed into 40-50 mesh tobacco powder after the moisture is less than 8.0%, for standby.

[0012] Preferably, in the technical scheme, in step (2), the extraction solvent is a mixture of ethanol, water and sodium chloride in a mass ratio of 50:45:5; and the extraction condition is 85 DEG C for 4-10h.

[0013] Preferably, in the technical scheme, in step (2), the addition amount of the tobacco powder, the extraction solvent and the FG is 2 / 3 of the volume of the extraction tank, and after the FG is taken out, the 30% ethanol / water solution is used for cleaning 3-5 times.

[0014] Preferably, in the technical scheme, in step (3), the temperature of the reduced pressure concentration is 40-60 DEG C, the vacuum pressure is 50-100 Pa, and the concentration of the extract is 1.0-1.2g / mL.

[0015] Preferably, in the technical scheme, step (4) is specifically: the tobacco leaf essential oil is separated by using a 50nm ceramic membrane and a 50kDa organic membrane in sequence, the permeate is continuously concentrated by using a reverse osmosis technology, a concentrated solution is obtained, and the 50% ethanol / water is used for dilution to a concentration of 10%, which is the tobacco leaf tobacco essential oil.

[0016] Preferably, in the technical scheme, the tobacco leaves are Zimbabwe tobacco leaves.

[0017] A tobacco essential oil is prepared according to the preparation method of the tobacco essential oil.

[0018] The application of a tobacco essential oil in a cigarette.

[0019] Preferably, in the technical scheme, the application is to inject the tobacco essential oil into the cigarette to improve the tobacco aroma coordination, improve the aroma amount and richness, increase the sweet and smooth feeling, and reduce the stimulation.

[0020] The technical scheme has the following beneficial effects:

[0021] The present application uses an ethanol-sodium chloride-water solvent system and a dynamic reciprocating magnetic field assisted extraction technology, and combines the use of a reusable graphene nanometer roll wrapped with a ferroferric oxide nanoparticle composite material (FG), which not only improves the extraction efficiency and reduces the production cost, but also maintains the heat-sensitive components of the tobacco essential oil, optimizes the sensory quality and taste of the cigarette, and the Zimbabwe tobacco essential oil can be well coordinated with the cigarette aroma, significantly improves the smoke quality, softens the cigarette smoke, enriches the aroma, improves the sweet and smooth feeling and delicacy of the cigarette, reduces the stimulation, and has the potential for industrial production. DETAILED DESCRIPTION

[0022] Various exemplary embodiments of the present application will now be described in detail. It should be noted that the relative arrangement of components and steps, numerical expressions, and numerical values set forth in these embodiments are not limiting to the scope of the present application unless otherwise specifically stated.

[0023] Principles of the present application:

[0024] Dynamic reciprocating magnetic field assisted extraction

[0025] Ethanol-sodium chloride-water, which introduces metal ions, can effectively improve the magnetic effect of the solvent system under the action of the magnetic field, speed up the solvent extraction, and improve the extraction efficiency. The extraction rate of Zimbabwe tobacco essential oil prepared by this method is about 1.52%, which effectively saves the production cost, and the graphene nanoroll wrapped ferroferric oxide nanoparticle composite material (hereinafter referred to as FG) can be reused, which is convenient for industrial production and has good application potential.

[0026] For example, the magnetic field can change the surface tension, viscosity, density, conductivity, etc. of the system, thereby affecting the physical and chemical properties of the substance, and thereby realizing the separation of the system.

[0027] Strong magnetic field assisted high efficiency extraction of Zimbabwe tobacco leaf to obtain crude extract, and membrane separation to obtain Zimbabwe tobacco essential extract. After membrane separation, different components of the organic phase are obtained to prepare Zimbabwe essential oil.

[0028] Example 1

[0029] A preparation method of a tobacco essential oil, comprising the following steps:

[0030] 1. Take 500g of Zimbabwe tobacco leaves in 2020, keep them in an oven at 45℃ for 12h, crush them with a pulverizer, and then pass them through a 40-mesh sieve to obtain Zimbabwe tobacco powder;

[0031] 2. Prepare ethanol:water=1:1(mass ratio) as the extraction solvent W1; the mass ratio of tobacco powder to extraction solvent is 1:8;

[0032] 3. According to the above proportion, 30g of Zimbabwe tobacco powder and extraction solvent are respectively weighed and added to the extraction tank, heated to 85℃ for extraction for 4h, and then cooled and filtered to obtain Zimbabwe crude extract T1.

[0033] 4. Transfer the Zimbabwe crude extract to a concentration tank, and use a rotary evaporator to remove the organic solvent and water in the extract by vacuum distillation, to obtain Zimbabwe extract, wherein the temperature of vacuum concentration is 40℃, the vacuum pressure is 80Pa, and the concentration of Zimbabwe extract is 1.0g / mL.

[0034] 5, Zimbabwe extract using membrane separation, Zimbabwe tobacco essential oil, the extraction rate is 1.24%.

[0035] Specifically, 50 nm ceramic membrane, 50 kDa organic membrane is used in turn to separate Zimbabwe essential oil, the permeate is continued to be concentrated by reverse osmosis technology to obtain a concentrated solution, which is diluted to a concentration of 10% using 50% ethanol / water, namely Zimbabwe tobacco essential oil A1.

[0036] 6, Zimbabwe tobacco essential oil A1 is added to the blank heated cigarette using the essence and flavor injection instrument, the addition amount is 0.8% of the weight of the tobacco. After injection, keep in a constant temperature and humidity environment for 48h to obtain sample cigarette Y1.

[0037] Example 2

[0038] 1, the mass ratio of ethanol-water-sodium chloride is 50:45:5 to prepare a solution as an extraction solvent W2; the mass ratio of tobacco dust and extract solvent is 1:10; the addition amount of FG is 1‰ of the total mass, and the filter cloth is wrapped.

[0039] 2, according to the above proportion, 30g of Zimbabwe tobacco dust, extraction solvent W2 and FG are weighed and added to the extraction tank, heated to 85℃ for 4h, cooled and filtered after extraction to obtain Zimbabwe crude extract T2.

[0040] 3, Zimbabwe crude extract T2 is transferred to the concentration tank, and the organic solvent and water in the extract are removed by rotary evaporator using vacuum distillation, to obtain Zimbabwe extract, wherein the temperature of vacuum concentration is 40℃, the vacuum pressure is 80Pa, and the concentration of Zimbabwe extract is 1.0g / mL.

[0041] 4, Zimbabwe extract using membrane separation, Zimbabwe tobacco essential oil, the extraction rate is 1.52%.

[0042] Specifically, 50 nm ceramic membrane, 50 kDa organic membrane is used in turn to separate Zimbabwe essential oil, the permeate is continued to be concentrated by reverse osmosis technology to obtain a concentrated solution, which is diluted to a concentration of 10% using 50% ethanol / water, namely Zimbabwe tobacco essential oil A2.

[0043] 5, Zimbabwe tobacco essential oil A2 is added to the blank heated cigarette using the essence and flavor injection instrument, the addition amount is 0.8% of the weight of the tobacco. After injection, keep in a constant temperature and humidity environment for 48h to obtain sample cigarette Y2.

[0044] Example 3

[0045] 1. Prepare a solution with an ethanol-water-sodium chloride mass ratio of 50:45:5 as the extraction solvent W2; the mass ratio of tobacco dust to extract solvent is 1:10; the amount of FG added is 1‰ of the total mass, and the solution is wrapped with filter cloth.

[0046] 2. Weigh 30g of Zimbabwean tobacco powder, extraction solvent W2 and FG according to the above proportions and add them to the extraction tank. Heat to 85℃ and extract for 4 hours. After extraction, cool and filter to obtain Zimbabwean crude extract T2.

[0047] 3. Transfer the crude Zimbabwean extract T2 to a concentration tank, and remove the organic solvent and water from the extract using a rotary evaporator by vacuum distillation to obtain Zimbabwean extract. The vacuum concentration temperature is 40°C, the vacuum pressure is 80 Pa, and the concentration of Zimbabwean extract is 1.0 g / mL.

[0048] 4. Dilute the Zimbabwean extract with 50% ethanol / water to a concentration of 10% to obtain Zimbabwean extract G3, with an extraction rate of 36.50%.

[0049] 5. Using a flavoring and fragrance injection apparatus, Zimbabwean tobacco extract G3 was added to blank heated cigarettes at an amount of 0.8% of the tobacco weight. After injection, the mixture was kept in a constant temperature and humidity environment for 48 hours to obtain sample cigarette Y3.

[0050] The sample smoke from Examples 1-3 was evaluated, and the results are shown in Table 1.

[0051] Table 1 Evaluation Results

[0052]

[0053] In summary, the tobacco essential oil prepared in this application, when applied to heated cigarettes, can not only effectively impart the natural aroma of tobacco to heated cigarettes, but also enhance the taste and flavor of heated cigarettes, improve the overall aroma quality, and reduce irritation and residue.

[0054] Although the present invention has been disclosed above with reference to embodiments, it is not intended to limit the present invention. Any person skilled in the art can make various different choices and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention is defined by the claims and their equivalents.

Claims

1. A method for preparing a tobacco essential oil, characterized by, The method comprises the following steps: (1) drying the tobacco leaves to a moisture content of less than 5%, and crushing the tobacco leaves into tobacco powder; (2) adding the tobacco powder, an extraction solvent, and graphene nanoroll-wrapped ferroferric oxide nanoparticle composite material into an extraction tank for extraction, and then cooling and filtering the extraction to obtain a crude extract; the mass ratio of the tobacco powder to the extraction solvent is 1:10, and the graphene nanoroll-wrapped ferroferric oxide nanoparticle composite material is added in an amount of 1 ‰ of the total mass; the extraction solvent is a mixture of ethanol, water, and sodium chloride in a mass ratio of 50:45:5; the extraction conditions are 85°C for 4 hours; and the tobacco leaves are Zimbabwe tobacco leaves; (3) transferring the crude extract to a concentration tank, and concentrating under reduced pressure to remove the organic solvent and water, thereby obtaining a tobacco extract; the temperature of the reduced-pressure concentration is 40°C, the vacuum pressure is 80 Pa, and the concentration of the tobacco extract is 1.0 g / mL; (4) obtaining tobacco essential oil from the tobacco extract by using a membrane separation technology; specifically, the tobacco essential oil is separated by using a 50 nm ceramic membrane and a 50 kDa organic membrane in sequence, the permeate is further concentrated by using a reverse osmosis technology, a concentrated solution is obtained, and the concentrated solution is diluted to a concentration of 10% to obtain the tobacco essential oil.

2. The method of preparing a tobacco essential oil according to claim 1, characterized by, Step (1) specifically comprises: placing the tobacco leaves in an oven at 40-50°C for 12-24 hours until the moisture content is less than 5.0%, and then crushing the tobacco leaves into tobacco powder with a particle size of 40-50 mesh for standby use.

3. The method of claim 1, wherein the tobacco essential oil is prepared by the process of, In step (2), the addition amount of the tobacco powder, the extraction solvent, and the graphene nanoroll-wrapped ferroferric oxide nanoparticle composite material is 2 / 3 of the volume of the extraction tank.

4. A tobacco essential oil, characterized in that, The tobacco essential oil is prepared according to the method for preparing the tobacco essential oil according to any one of claims 1-3.

5. The tobacco essential oil according to claim 4 is applied to cigarettes.

6. Use according to claim 5, characterized in that, The application is to inject the tobacco essential oil into cigarettes to improve the harmony of tobacco aroma, increase the amount and richness of aroma, increase the sweet and smooth feeling, and reduce irritation.

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