Toothpaste containing active superoxide dismutase and preparation method thereof

Abstract

The invention relates to toothpaste containing active superoxide dismutase and a preparation method of the toothpaste, and belongs to the field of oral care. The toothpaste containing the active superoxide dismutase is prepared from the following components in percentage by mass: 20 to 45 percent of abrasive, 20 to 55 percent of humectant, 0.1 to 3.5 percent of thickening agent, 0.1 to 7 percent of foaming agent, 0.01 to 5 percent of sweetening agent, 0.01 to 2 percent of coated yeast superoxide dismutase powder, 0.1 to 0.5 percent of preservative, 0.05 to 1.5 percent of functional additive, 0.1 to 1.5 percent of essence and the balance of water. Carboxyl of lauric acid and hydroxyl of sodium alginate form an in-situ esterification reaction to form a wrapping layer on the surface of the mixture, so that the stability of the surface of the mixture is improved, and inactivation of yeast superoxide dismutase powder and volatilization of notopterygium volatile oil are avoided.

Description

Technical Field

[0001] The invention belongs to the technical field of oral care, and in particular relates to a toothpaste containing active superoxide dismutase and a preparation method thereof. Background Art

[0002] In recent years, as people have become more aware of oral health, the demand for toothpaste has also been increasing year by year, especially toothpaste with functional ingredients, which is very popular among people. Consumers have higher requirements for functional toothpaste that has certain therapeutic effects on oral problems. Therefore, products containing active superoxide dismutase (SOD) are in increasing demand under this background. The invention patent with application publication number CN113749970A discloses a toothpaste containing superoxide dismutase SOD for promoting teeth whitening and a preparation method thereof. The toothpaste containing superoxide dismutase SOD for promoting teeth whitening comprises water and the following components in percentage by mass: flavor 0.004%-0.008%, superoxide dismutase 0.846%-1.272%, foaming agent 2%-4%, moisturizing agent 18.6%-21.5%, xylitol 0.01%-0.02%, abrasive 35%-45%, thickener 0.64%-1.2%; sodium fluoride 0.1%-2%; methyl paraben 0.1%, but the SOD enzyme is unstable, and the preservatives and surfactants in the toothpaste may react with the SOD enzyme to cause the SOD enzyme to be inactivated. The toothpaste containing superoxide dismutase SOD for promoting teeth whitening cannot solve the problem of easy inactivation of the SOD enzyme. Summary of the invention

[0003] The first object of the present invention is to provide a toothpaste containing active superoxide dismutase to solve the technical problem that the SOD enzyme in the toothpaste is easily inactivated.

[0004] The second object of the present invention is to provide a method for preparing toothpaste containing active superoxide dismutase.

[0005] In order to achieve the above purpose, the technical solution adopted by the present invention is: A toothpaste containing active superoxide dismutase, comprising the following substances by mass percentage: 20-45% abrasive, 20-55% moisturizer, 0.1-3.5% thickener, 0.1-7% foaming agent, 0.01-5% sweetener, 0.01-2% coated yeast superoxide dismutase powder, 0.1-0.5% preservative, 0.05-1.5% functional additive, 0.1-1.5% flavor, and the balance is water; The preparation method of the coated yeast superoxide dismutase powder is as follows: S1: mixing volatile oil of Notopterygium wilfordii and yeast superoxide dismutase powder evenly to obtain a mixture, dissolving sodium alginate in water to obtain solution 1, spraying solution 1 onto the surface of the mixture, and drying; S2: dissolving lauric acid in an organic solvent to obtain a second solution, spraying the second solution onto the surface of the dried mixture in S1, introducing an acidic gas to react, and then drying; S3: dispersing polydopamine nanoparticles in ethanol to obtain a dispersion, spraying the dispersion onto the surface of the dried mixture in S2, and drying; S4: Mix the composite colloid, soluble calcium salt and water to obtain a mixed solution, put the dried mixture in S3 into the mixed solution, and then place it in a calcium gluconate solution for immersion.

[0006] Furthermore, the acidic gas in S2 is hydrogen chloride gas, the reaction time is 1 to 2 hours, and the organic solvent is ether or ethanol.

[0007] Furthermore, the spray volume of the spray in S1 is 2 to 4 mL / cm 2 The spray volume of the spray in S2 is 2 to 4 mL / cm 2 .

[0008] Furthermore, the concentration of solution 1 in S1 is 1-2 mg / mL, the concentration of solution 2 in S2 is 60-80 mg / mL; and the concentration of polydopamine nanoparticles in the dispersion in S3 is 8-10 mg / mL.

[0009] Furthermore, the mass ratio of the Notopterygium wilfordii volatile oil to the yeast superoxide dismutase powder in S1 is 0.5-0.7:1.

[0010] Furthermore, the composite colloid in S4 includes guar gum, konjac gel and whey protein, and the mass ratio of the guar gum, konjac gel and whey protein is 3-5:8-10:15-20; the soluble calcium salt is calcium lactate; and the mass fraction of the calcium gluconate solution is 5-10%.

[0011] Furthermore, the mass ratio of the composite colloid to the soluble calcium salt in the mixed solution in S4 is 1:3-4; the mass ratio of the composite colloid to the water is 1:600-800.

[0012] Furthermore, the abrasive is one or more of silicon dioxide, stone powder, calcium hydrogen phosphate, and aluminum hydroxide; the moisturizer is one or more of sorbitol, glycerin, propylene glycol, and polyethylene glycol; the foaming agent is one or more of coconut fatty acid monoethanolamide, lauryl glucoside, sodium lauroyl glutamate, and poloxamer; the thickener is one or more of sodium carboxymethyl cellulose, xanthan gum, hydroxypropyl guar gum, carrageenan, and lithium magnesium silicate; the sweetener is one or more of xylitol, sucralose, sodium saccharin, erythritol, mannitol, dipotassium glycyrrhizate, and acesulfame potassium; the preservative is one or more of sodium benzoate, sodium methyl ester, and potassium sorbate.

[0013] Furthermore, the functional additive is one or more of lactobacillus, oligofructose and glucose; the flavor is one or more of fruit flavor, flower flavor, tea flavor, mint flavor and candy flavor.

[0014] A method for preparing a toothpaste containing active superoxide dismutase comprises the following steps: S1: mixing a friction agent and a thickener to obtain a solid material; mixing a moisturizer, water, a sweetener, a preservative, a functional additive and coated yeast superoxide dismutase powder to obtain a liquid material 1; dissolving a foaming agent in water to obtain a liquid material 2; S2: Open the vacuum system of the paste making machine, reduce the vacuum degree to -0.06~-0.03 MPa, introduce liquid material 1, and start the low-speed stirrer for stirring; after the liquid material 1 is fed, reduce the vacuum degree to -0.085~-0.09MPa, introduce solid material, and start the high-speed stirrer at the same time, and keep the vacuum degree at -0.06~-0.085 MPa until the solid material is introduced, homogenize, introduce liquid material 2 after 15~20 minutes, continue homogenizing for 5~8 minutes, cool to 20~50 ℃, add flavor, and homogenize for 8~10 minutes; degas to a vacuum degree of -0.092~0.098 MPa, and keep it for 10~18 minutes; S3: The ointment comes out.

[0015] Beneficial effects of the present invention: The carboxyl of lauric acid of the present invention and the hydroxyl of sodium alginate form an in-situ esterification reaction to form a coating layer on the surface of the mixture, thereby improving the stability of the surface of the mixture and avoiding the inactivation of yeast superoxide dismutase powder and the volatilization of Notopterygium indici volatile oil. At the same time, the carboxyl of lauric acid and the hydroxyl of sodium alginate form an in-situ esterification reaction as a reversible reaction. When brushing teeth, due to friction and water flow, water molecules can enter the interior of the esterification system to release yeast superoxide dismutase powder and Notopterygium indici volatile oil. The outer layer composed of composite colloid and soluble calcium salt breaks under the action of friction, and the polydopamine nanoparticles of the middle layer can adhere to the oral cavity. Relying on the adhesion of the polydopamine nanoparticles and the reversibility of the esterification reaction, the yeast superoxide dismutase powder and Notopterygium indici volatile oil can be slowly released in the oral cavity, and play a role for a long time.

[0016] The volatile oil of Notopterygium wilfordii of the present invention has the function of resisting Candida albicans, and the toothpaste containing active superoxide dismutase of the present invention can alleviate and prevent oral problems caused by Candida albicans.

[0017] The notopterygium root volatile oil and yeast superoxide dismutase powder of the present invention both have anti-inflammatory and anti-oxidative effects, and the combination of the two can achieve an effect of 1+1>2. DETAILED DESCRIPTION

[0018] The present invention will be further described below in conjunction with the embodiments of the present invention.

[0019] The volatile oil of Notopterygium wilfordii is extracted by supercritical carbon dioxide extraction. Example 1

[0020] The toothpaste containing active superoxide dismutase of Example 1 includes the following raw materials calculated by mass percentage: 40 g abrasive, 31 g moisturizer, 1.3 g thickener, 4 g foaming agent, 0.55 g sweetener, 0.21 g coated yeast superoxide dismutase powder, 0.4 g preservative, 0.05 g functional additive, 0.85 g flavor, and 21.64 g water.

[0021] The friction agent is calcium hydrogen phosphate; the moisturizer includes sorbitol 25 g, glycerin 5 g, propylene glycol 1 g; the thickener includes xanthan gum 0.6 g, hydroxypropyl guar gum 0.5 g, and lithium magnesium silicate 0.2 g; the foaming agent is lauryl glucoside 1.5 g, sodium lauroyl glutamate 2 g, and poloxamer 0.5 g; the sweetener is xylitol 0.5 g and sucralose 0.05 g; the preservative is potassium sorbate; the functional additive is lactobacillus; the flavor is mint flavor. The poloxamer is poloxamer 407.

[0022] The preparation method of coated yeast superoxide dismutase powder is as follows: S1: 0.5 g of Notopterygium root volatile oil and 1 g of yeast superoxide dismutase powder were mixed evenly to obtain a mixture, sodium alginate was dissolved in water to obtain solution 1, solution 1 was sprayed onto the surface of the mixture and dried; the concentration of solution 1 was 1 mg / mL; the spray volume was 3 mL / cm 2 ; S2: Lauric acid was dissolved in ethanol to obtain solution 2, which was sprayed onto the surface of the dried mixture in S1, and hydrogen chloride gas was introduced to react for 1 h, followed by drying; the concentration of solution 2 was 60 mg / mL; the spray volume was 3 mL / cm 2 ; S3: dispersing polydopamine nanoparticles in ethanol to obtain a dispersion, spraying the dispersion onto the surface of the dried mixture in S2, and drying; the concentration of polydopamine nanoparticles in the dispersion is 8 mg / mL; S4: 1 g of the composite colloid, 4 g of calcium lactate and 800 g of water are mixed to obtain a mixed solution, the dried mixture in S3 is placed in the mixed solution, and then immersed in a calcium gluconate solution with a mass fraction of 7%. The composite colloid includes guar gum, konjac gel and whey protein, and the mass ratio of guar gum, konjac gel and whey protein is 5:10:20.

[0023] The preparation method of the toothpaste containing active superoxide dismutase in Example 1 is: S1: Mix the calcium hydrogen phosphate, xanthan gum, hydroxypropyl guar gum and lithium magnesium silicate of the powder phase to obtain a solid material, and place it in a powder tank for standby use; mix sorbitol, glycerol, propylene glycol, sucralose, xylitol, potassium sorbate, coated yeast superoxide dismutase powder, lactobacillus and water to obtain a liquid material 1; dissolve lauryl glucoside and poloxamer in water to obtain a liquid material 2; weigh sodium lauroyl glutamate separately according to the formula and set aside; S2: Open the vacuum system of the paste making machine and reduce the vacuum degree to -0.05 MPa; introduce liquid material 1 and start the low-speed agitator at the same time. Then reduce the vacuum degree to -0.085 MPa and start introducing solid materials, while turning on the high-speed agitator and maintaining the vacuum degree at -0.06 MPa until the solid materials are fed; homogenize in the paste making machine for 20 minutes, then introduce liquid material 2 and sodium lauroyl glutamate, and homogenize for another 5 minutes; when the temperature drops to 50 °C, introduce mint essence and homogenize for another 10 minutes; degas to a vacuum degree of -0.092 MPa and maintain this vacuum value for 15 minutes; S3: Open the normal pressure valve, release the air, and discharge the paste after passing the inspection. Example 2

[0024] The toothpaste containing active superoxide dismutase of Example 2 includes the following raw materials calculated by mass percentage: 45 kg of abrasive, 25 kg of moisturizer, 3 kg of thickener, 6 kg of foaming agent, 5 kg of sweetener, 0.05 kg of coated yeast superoxide dismutase powder, 0.5 kg of preservative, 1 kg of functional additive, 1.5 kg of flavor, and 12.95 kg of water.

[0025] Among them, the friction agent is aluminum hydroxide; the moisturizing agent is sorbitol 10 kg, glycerin 10 kg, propylene glycol 5 kg; the thickener is xanthan gum 1 kg, hydroxypropyl guar gum 1 kg, carrageenan 1 kg; the foaming agent is lauryl glucoside 2 kg, sodium lauroyl glutamate 3 kg, poloxamer 1 kg; the sweetener is acesulfame potassium 2 kg, dipotassium glycyrrhizinate 3 kg; the preservative is sodium methyl ester; the functional additive is glucose; the flavor is tea flavor. The poloxamer is poloxamer 407.

[0026] The preparation method of coated yeast superoxide dismutase powder is as follows: S1: 0.7 kg of Notopterygium wilfordii essential oil and 1 kg of yeast superoxide dismutase powder were mixed evenly to obtain a mixture, sodium alginate was dissolved in water to obtain solution 1, solution 1 was sprayed onto the surface of the mixture and dried; the concentration of solution 1 was 2 mg / mL; the spray volume was 4 mL / cm 2 ; S2: Lauric acid was dissolved in ethanol to obtain solution 2, which was sprayed onto the surface of the dried mixture in S1, and hydrogen chloride gas was introduced to react for 2 h, followed by drying; the concentration of solution 2 was 70 mg / mL; the spray volume was 4 mL / cm 2 ; S3: dispersing polydopamine nanoparticles in ethanol to obtain a dispersion, spraying the dispersion onto the surface of the dried mixture in S2, and drying; the concentration of polydopamine nanoparticles in the dispersion is 10 mg / mL; S4: 1 kg of composite colloid, 3 kg of calcium lactate and 600 kg of water are mixed to obtain a mixed solution, the dried mixture in S3 is placed in the mixed solution, and then immersed in a calcium gluconate solution with a mass fraction of 10%. The composite colloid includes guar gum, konjac gel and whey protein, and the mass ratio of guar gum, konjac gel and whey protein is 3:8:15.

[0027] The preparation method of the toothpaste containing active superoxide dismutase of Example 2 comprises the following steps: S1: Mix the powder phase aluminum hydroxide, xanthan gum, hydroxypropyl guar gum and carrageenan to obtain a solid material, and place it in a powder tank for standby use; mix sorbitol, glycerol, propylene glycol, acesulfame potassium, dipotassium glycyrrhizinate, sodium methyl ester, coated yeast superoxide dismutase powder, glucose and water to obtain a liquid material 1; dissolve lauryl glucoside and poloxamer in hot water to obtain a liquid material 2; weigh sodium lauroyl glutamate separately according to the formula and set aside; S2: Open the vacuum system of the paste making machine and reduce the vacuum degree to -0.06 MPa; introduce liquid material 1 and start the low-speed agitator at the same time. Then reduce the vacuum degree to -0.087 MPa and start introducing solid materials, while turning on the high-speed agitator and maintaining the vacuum degree at -0.07 MPa until the solid materials are fed; homogenize in the paste making machine for 15 minutes, then introduce liquid material 2 and sodium lauroyl glutamate, and homogenize for another 7 minutes; when the temperature drops to 30°C, introduce tea essence and homogenize for another 8 minutes; degas to a vacuum degree of -0.095 MPa and maintain this vacuum value for 10 minutes; S3: Open the normal pressure valve, release the air, and discharge the paste after passing the inspection. Example 3

[0028] The toothpaste containing active superoxide dismutase of Example 3 includes the following raw materials calculated by mass percentage: 20 kg of abrasive, 55 kg of moisturizer, 0.3 kg of thickener, 0.3 kg of foaming agent, 2 kg of sweetener, 2 kg of coated yeast superoxide dismutase powder, 0.1 kg of preservative, 1.5 kg of functional additives, 0.1 kg of flavor, and 18.7 kg of water.

[0029] The friction agent is silicon dioxide; the moisturizing agent is sorbitol 20 kg, glycerin 20 kg, polyethylene glycol 15 kg; the thickener is xanthan gum 0.1 kg, sodium carboxymethyl cellulose 0.1 kg, carrageenan 0.1 kg; the foaming agent is lauryl glucoside 0.1 kg, sodium lauroyl glutamate 0.1 kg, poloxamer 0.1 kg; the sweetener is mannitol 1 kg, erythritol 1 kg; the preservative is sodium benzoate; the functional additive is oligofructose; the flavor is fruit flavor. The poloxamer is poloxamer 407.

[0030] The preparation method of coated yeast superoxide dismutase powder is as follows: S1: 0.6 kg of Notopterygium incisum volatile oil and 1 kg of yeast superoxide dismutase powder were mixed evenly to obtain a mixture, sodium alginate was dissolved in water to obtain solution 1, solution 1 was sprayed onto the surface of the mixture and dried; the concentration of solution 1 was 1.5 mg / mL; the spray volume was 2 mL / cm 2 ; S2: Dissolve lauric acid in ethanol to obtain solution 2, spray solution 2 onto the surface of the dried mixture in S1, introduce hydrogen chloride gas to react for 2 h, and then dry; the concentration of solution 2 is 80 mg / mL; the spray volume is 2 mL / cm 2 ; S3: dispersing polydopamine nanoparticles in ethanol to obtain a dispersion, spraying the dispersion onto the surface of the dried mixture in S2, and drying; the concentration of polydopamine nanoparticles in the dispersion is 9 mg / mL; S4: 1 kg of composite colloid, 3 kg of calcium lactate and 700 kg of water are mixed to obtain a mixed solution, the dried mixture in S3 is placed in the mixed solution, and then immersed in a calcium gluconate solution with a mass fraction of 10%. The composite colloid includes guar gum, konjac gel and whey protein, and the mass ratio of guar gum, konjac gel and whey protein is 4:9:18.

[0031] The preparation method of the toothpaste containing active superoxide dismutase in Example 3 is: S1: Mix the powder phase silicon dioxide, xanthan gum, sodium carboxymethyl cellulose and carrageenan to obtain a solid material, and place it in a powder tank for standby use; mix sorbitol, glycerol, polyethylene glycol, mannitol, erythritol, sodium benzoate, coated yeast superoxide dismutase powder, oligofructose and water to obtain a liquid material 1; dissolve lauryl glucoside and poloxamer in hot water to obtain a liquid material 2; weigh sodium lauroyl glutamate separately according to the formula and set aside; S2: Open the vacuum system of the paste making machine and reduce the vacuum degree to -0.03 MPa; introduce liquid material 1 and start the low-speed agitator at the same time. Then reduce the vacuum degree to -0.089 MPa and start introducing solid materials, while turning on the high-speed agitator and maintaining the vacuum degree at -0.085 MPa until the solid materials are fed; homogenize in the paste making machine for 18 minutes, then introduce liquid material 2 and sodium lauroyl glutamate, and homogenize for another 8 minutes; when the temperature drops to 20 °C, introduce fruit essence and homogenize for another 10 minutes; degas to a vacuum degree of -0.098 MPa and maintain this vacuum value for 18 minutes; S3: Open the normal pressure valve, release the air, and discharge the paste after passing the inspection.

[0032] Comparative Example 1 The formula of the toothpaste containing active superoxide dismutase in Comparative Example 1 is the same as that in Example 1, and the preparation method of the toothpaste containing active superoxide dismutase in Comparative Example 1 is the same as that in Example 1. The difference between the toothpaste containing active superoxide dismutase in Comparative Example 1 and Example 1 is that the preparation method of the coated yeast superoxide dismutase powder in Comparative Example 1 is: The preparation method of coated yeast superoxide dismutase powder is as follows: S1: Sodium alginate was dissolved in water to obtain solution 1, which was sprayed onto the surface of yeast superoxide dismutase powder and dried; the concentration of solution 1 was 1 mg / mL; the spray volume was 3 mL / cm 2 ; S2: Dissolve lauric acid in ethanol to obtain solution 2, spray solution 2 onto the surface of the dried yeast superoxide dismutase powder in S1, introduce hydrogen chloride gas to react for 1 h, and then dry; the concentration of solution 2 is 60 mg / mL; the spray volume is 3 mL / cm 2 ; S3: dispersing polydopamine nanoparticles in ethanol to obtain a dispersion, spraying the dispersion onto the surface of the dried yeast superoxide dismutase powder in S2, and drying; the concentration of polydopamine nanoparticles in the dispersion is 8 mg / mL; S4: 1 g of the composite colloid, 4 g of calcium lactate and 800 g of water are mixed to obtain a mixed solution, the dried mixture in S3 is placed in the mixed solution, and then immersed in a calcium gluconate solution with a mass fraction of 7%. The composite colloid includes guar gum, konjac gel and whey protein, and the mass ratio of guar gum, konjac gel and whey protein is 5:10:20.

[0033] The preparation method of the toothpaste containing active superoxide dismutase in Comparative Example 1 is the same as that in Example 1.

[0034] Test Example 1 The superoxide dismutase activity of the toothpaste containing active superoxide dismutase of Example 1 was tested, and the test results are shown in Table 1.

[0035] Table 1 Superoxide dismutase activity test results of Example 1

[0036] Test Example 2 After brushing teeth for 0 h, 2 h, 4 h, 6 h, and 8 h, saliva from the inner wall of the oral cavity was extracted, and the saliva was centrifuged to remove protein substances. After extraction, the supernatant was centrifuged and dried with nitrogen, and then re-dissolved with methanol, vortexed, centrifuged, and the supernatant was taken and filtered with a filter membrane. The filtrate was taken to detect the residual amount of yeast superoxide dismutase and Notopterygium incisum volatile oil in the filtrate. The test results are shown in Table 2. The residual amount of Notopterygium incisum volatile oil was calculated based on the limonene content in Notopterygium incisum volatile oil.

[0037] Table 2 Residual amounts of yeast superoxide dismutase and volatile oil of Notopterygium in oral saliva after brushing teeth

[0038] As can be seen from Table 2, 8 hours after brushing, more than 40% of the yeast superoxide dismutase powder and Notopterygium root volatile oil in the oral saliva still remain after 8 hours, indicating that the toothpaste containing active superoxide dismutase of the present invention can adhere to the oral cavity within 8 hours of brushing and continue to play the role of anti-inflammatory, anti-Candida albicans and oral mucosa repair.

[0039] Test Example 3 The sample concentrations of screening Example 1 and Comparative Example 1 were 0.0700 mg / mL (m / v), 0.0350 mg / mL (m / v), and 0.0175 mg / mL (m / v) as the test concentrations for the anti-inflammatory efficacy experiment, and the experimental results are shown in Table 3. Wherein LPS is lipopolysaccharide, and PG is Porphyromonas gingivalis.

[0040] Table 3 Anti-inflammatory efficacy test results of Example 1 and Comparative Example 1

[0041] # indicates that the difference is statistically significant compared with the negative control group (p < 0.05); *Indicates that the difference is statistically significant compared with the model control group (p<0.05).

[0042] The effects of Example 1 and Comparative Example 1 on the interleukin-1ɑ (IL-1ɑ) content in the HGE cell inflammation model induced by LPS-PG are shown in Table 4.

[0043] Table 4 Effects of Example 1 and Comparative Example 1 on IL-1α content in LPS-PG-induced human gingival epithelial cell inflammation model (HGE cell inflammation model)

[0044] # indicates that the difference is statistically significant compared with the negative control group (p < 0.05); *Indicates that the difference is statistically significant compared with the model control group (p<0.05).

[0045] As can be seen from Tables 3 and 4, the relative content of PGE-2 in the model control group (M) was significantly increased compared with the negative control group (NC) (P<0.05); the relative content of PGE-2 in the positive control group was significantly decreased compared with the model control group (P<0.05), indicating that the modeling was successful. Compared with the model control group, the relative content of PGE-2 in Example 1 decreased, and there was a significant difference (p<0.05). Compared with the negative control group (NC), the relative content of IL-1ɑ in the model control group (M) was significantly increased (P<0.05); compared with the model control group, the relative content of IL-1ɑ in the positive control group was significantly decreased (P<0.05), indicating that the modeling was successful. Compared with the model control group, the relative content of IL-1ɑ in Example 1 decreased, and there was a significant difference (p<0.05). In summary, the toothpaste containing active superoxide dismutase in Example 1 shows anti-inflammatory and gingival protection effects. In addition, it can be seen from Tables 3 and 4 that the anti-inflammatory and gingival protection effect of the toothpaste containing active superoxide dismutase in Example 1 is better than the anti-inflammatory and gingival protection effect of Comparative Example 1, indicating that the compounding effect of the yeast superoxide dismutase powder and Notopterygium incisum volatile oil in Example 1 is better than the effect of using the yeast superoxide dismutase powder alone, and the yeast superoxide dismutase powder and Notopterygium incisum volatile oil have a compounding effect.

[0046] Test Example 4 According to WS / T 650-2019 “Evaluation Method for Antibacterial and Antibacterial Effects”, the toothpaste containing active superoxide dismutase in Example 1 was subjected to an antibacterial test on Candida albicans for 5 minutes under experimental conditions. The results are shown in Table 5.

[0047] Table 5 Antibacterial effect of toothpaste containing active superoxide dismutase in Example 1 on Candida albicans

[0048] It can be seen from Table 5 that the toothpaste containing active superoxide dismutase of the present invention has a good antibacterial effect on Candida albicans.

Claims

1. A toothpaste containing active superoxide dismutase, characterized in that: The following substances are included by mass percentage: abrasive 20-45%, moisturizer 20-55%, thickener 0.1-3.5%, foaming agent 0.1-7%, sweetener 0.01-5%, coated yeast superoxide dismutase powder 0.01-2%, preservative 0.1-0.5%, functional additive 0.05-1.5%, flavor 0.1-1.5%, and the balance is water; The preparation method of the coated yeast superoxide dismutase powder is as follows: S1: mixing volatile oil of Notopterygium wilfordii and yeast superoxide dismutase powder evenly to obtain a mixture, dissolving sodium alginate in water to obtain solution 1, spraying solution 1 onto the surface of the mixture, and drying; S2: dissolving lauric acid in an organic solvent to obtain a second solution, spraying the second solution onto the surface of the dried mixture in S1, introducing an acidic gas to react, and then drying; S3: dispersing polydopamine nanoparticles in ethanol to obtain a dispersion, spraying the dispersion onto the surface of the dried mixture in S2, and drying; S4: Mix the composite colloid, soluble calcium salt and water to obtain a mixed solution, put the dried mixture in S3 into the mixed solution, and then place it in a calcium gluconate solution for immersion.

2. The toothpaste containing active superoxide dismutase according to claim 1, characterized in that: The acidic gas in S2 is hydrogen chloride gas, the reaction time is 1 to 2 hours, and the organic solvent is ether or ethanol.

3. The toothpaste containing active superoxide dismutase according to claim 1, characterized in that: The spray volume of the spray in S1 is 2 to 4 mL / cm 2 The spray volume of the spray in S2 is 2 to 4 mL / cm 2 .

4. The toothpaste containing active superoxide dismutase according to claim 1, characterized in that: The concentration of solution 1 in S1 is 1-2 mg / mL, the concentration of solution 2 in S2 is 60-80 mg / mL; the concentration of polydopamine nanoparticles in the dispersion in S3 is 8-10 mg / mL.

5. The toothpaste containing active superoxide dismutase according to claim 1, characterized in that: The mass ratio of the Notopterygium wilfordii volatile oil to the yeast superoxide dismutase powder in S1 is 0.5-0.7:

1.

6. The toothpaste containing active superoxide dismutase according to claim 1, characterized in that: The composite colloid in S4 includes guar gum, konjac gel and whey protein, and the mass ratio of the guar gum, konjac gel and whey protein is 3-5:8-10:15-20; the soluble calcium salt is calcium lactate; and the mass fraction of the calcium gluconate solution is 5-10%.

7. The toothpaste containing active superoxide dismutase according to claim 1, characterized in that: The mass ratio of the composite colloid to the soluble calcium salt in the mixed solution in S4 is 1:3-4; the mass ratio of the composite colloid to the water is 1:600-800.

8. The toothpaste containing active superoxide dismutase according to claim 1, characterized in that: The abrasive is one or more of silicon dioxide, stone powder, calcium hydrogen phosphate, and aluminum hydroxide; the moisturizer is one or more of sorbitol, glycerin, propylene glycol, and polyethylene glycol; the foaming agent is one or more of coconut fatty acid monoethanolamide, lauryl glucoside, sodium lauroyl glutamate, and poloxamer; the thickener is one or more of sodium carboxymethyl cellulose, xanthan gum, hydroxypropyl guar gum, carrageenan, and lithium magnesium silicate; the sweetener is one or more of xylitol, sucralose, sodium saccharin, erythritol, mannitol, dipotassium glycyrrhizate, and acesulfame potassium; the preservative is one or more of sodium benzoate, sodium methyl ester, and potassium sorbate.

9. The toothpaste containing active superoxide dismutase according to claim 1, characterized in that: The functional additive is one or more of lactobacillus, oligofructose and glucose; the flavor is one or more of fruit flavor, flower flavor, tea flavor, mint flavor and candy flavor.

10. A method for preparing a toothpaste containing active superoxide dismutase as claimed in claim 1, characterized in that: The following steps are involved: S1: mixing a friction agent and a thickener to obtain a solid material; mixing a moisturizer, water, a sweetener, a preservative, a functional additive and coated yeast superoxide dismutase powder to obtain a liquid material 1; dissolving a foaming agent in water to obtain a liquid material 2; S2: Open the vacuum system of the paste making machine, reduce the vacuum degree to -0.06~-0.03 MPa, introduce liquid material 1, and start the low-speed stirrer for stirring; after the liquid material 1 is fed, reduce the vacuum degree to -0.085~-0.09 MPa, introduce solid material, and start the high-speed stirrer at the same time, and keep the vacuum degree at -0.06~-0.085 MPa until the solid material is introduced, homogenize, introduce liquid material 2 after 15~20 minutes, continue homogenizing for 5~8 minutes, cool to 20~50 ℃, add flavor, and homogenize for 8~10 minutes; degas to a vacuum degree of -0.092~0.098 MPa, and keep it for 10~18 minutes; S3: The ointment comes out.

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