Liposome of bionic fetal fat, preparation method and application

Abstract

The invention discloses a liposome of bionic fetal fat as well as a preparation method and application of the liposome. The liposome of the bionic fetal fat is prepared from the following raw materials in parts by weight: 10 to 20 parts of hydrogenated lecithin, 5 to 15 parts of amino acid, 5 to 15 parts of stearic acid, 1 to 5 parts of ceramide, 1 to 10 parts of tocopheryl acetate, 15 to 25 parts of squalane, 1 to 3 parts of palmitoyl tripeptide-8, 1 to 5 parts of plant polysaccharide, 1 to 10 parts of vegetable oil, 1 to 5 parts of hyaluronic acid grafted procyanidine and 10 to 30 parts of deionized water. The preparation method of the liposome of the bionic fetal fat comprises the following steps: S1, dispersing amino acid, palmitoyl tripeptide-8 and plant polysaccharide in deionized water to form a water phase; s2, uniformly mixing hydrogenated lecithin, stearic acid, ceramide, tocopheryl acetate, squalane and vegetable oil to form an oil phase; and S3, homogenizing the water phase in the S1 and the oil phase in the S2 in a micro-jet homogenizer to prepare the liposome of the bionic fetal fat. The liposome of the bionic fetal fat, disclosed by the invention, has excellent moisturizing performance.

Description

Technical Field

[0001] The invention relates to the technical field of bionic vernix caseosa, and in particular to a bionic vernix caseosa liposome, a preparation method and application thereof. Background Art

[0002] Bionic vernix caseosa is a substance that mimics the composition and function of natural vernix caseosa of human skin, and is usually composed of the following main components: fatty acids, triglycerides, phospholipids, cholesterol, natural vegetable oils and ceramides. Bionic vernix caseosa has the functions of moisturizing and nourishing, repairing skin barrier, enhancing skin softness and smoothness, anti-oxidation and anti-inflammatory, and can be applied to the fields of skin care products and cosmetics. The purpose of the present invention is to provide a liposome of bionic vernix caseosa with good moisturizing performance for use in cosmetics. Summary of the invention

[0003] Based on the technical problems existing in the background technology, the present invention proposes a bionic vernix caseosa liposome, a preparation method and an application thereof, which have excellent moisturizing properties.

[0004] The present invention provides a bionic vernix caseosa liposome, which comprises the following raw materials in parts by weight: 10-20 parts of hydrogenated lecithin, 5-15 parts of amino acids, 5-15 parts of stearic acid, 1-5 parts of ceramide, 1-10 parts of tocopherol acetate, 15-25 parts of squalane, 3 parts of palmitoyl tripeptide-81, 1-5 parts of plant polysaccharides, 1-5 parts of hyaluronic acid grafted proanthocyanidins, 1-10 parts of vegetable oil, and 10-30 parts of deionized water.

[0005] Preferably, the ceramide consists of ceramide EOP, ceramide NP and ceramide AS in a mass ratio of 1:0.5-2:0.5-2.

[0006] Preferably, the plant polysaccharide is composed of Bletilla striata polysaccharide, Dendrobium officinale polysaccharide and Osmanthus fragrans leaf polysaccharide in a mass ratio of 3:1-9:1-9.

[0007] Preferably, the vegetable oil is one or more of tea oil, coconut oil, olive oil and shea butter.

[0008] Preferably, the amino acid is one or more of glycine, glutamic acid, aspartic acid, alanine, proline, serine, lysine, tryptophan and cysteine.

[0009] The method for preparing the bionic vernix caseosa liposomes proposed by the present invention comprises the following steps:

[0010] S1: dispersing amino acids, palmitoyl tripeptide-8, hyaluronic acid-grafted proanthocyanidins and plant polysaccharides in deionized water to form an aqueous phase;

[0011] S2: mixing hydrogenated lecithin, stearic acid, ceramide, tocopheryl acetate, squalane and vegetable oil evenly to form an oil phase;

[0012] S3: The water phase of S1 and the oil phase of S2 are homogenized in a microfluidizer to prepare bionic vernix caseosa liposomes.

[0013] Preferably, the homogenization temperature in S3 is 30-60° C., and the homogenization pressure is 10,000-20,000 psi.

[0014] The present invention provides the application of the bionic vernix caseosa liposome in skin care products.

[0015] Beneficial technical effects of the present invention:

[0016] The bionic vernix caseosa liposomes of the present invention are composed of hydrogenated lecithin, amino acids, stearic acid, ceramide, tocopherol acetate, squalane, palmitoyl tripeptide-8, plant polysaccharides and plant oils, and can be combined with the natural stratum corneum of the skin to enhance the integrity of the skin barrier. The bionic vernix caseosa liposomes can form a protective film on the skin surface to reduce the evaporation of water on the skin surface and lock in water. The protective film has a strong affinity with the skin and is not easily destroyed by the external environment, providing a long-lasting moisturizing effect. The palmitoyl tripeptide-8 and the like of the present invention also have anti-inflammatory, repairing, soothing and anti-aging effects, and have strong lipophilicity and can more easily penetrate into the skin. The ceramides of the present invention are composed of ceramide EOP, ceramide NP and ceramide AS. Compared with the use of a single type of The present invention also adds plant polysaccharides, and the plant polysaccharides are composed of bletilla striata polysaccharides, dendrobium officinale polysaccharides and osmanthus leaf polysaccharides, which have a synergistic promoting effect on improving the moisturizing performance of liposomes. This is because the combined effect of bletilla striata polysaccharides, dendrobium officinale polysaccharides and osmanthus leaf polysaccharides can significantly improve the hydration of the skin, maintain the moisture balance of the skin, and help to enhance the skin barrier function and reduce water loss; the liposomes of the present invention also add hyaluronic acid grafted proanthocyanidins, which themselves have certain moisturizing and antioxidant properties. At the same time, after the liposomes are homogenized by a microfluidizer, the hyaluronic acid grafted proanthocyanidins improve the stability of the liposomes due to hydrogen bonding and electrostatic interactions, thereby improving the encapsulation rate of the liposomes. DETAILED DESCRIPTION

[0017] The present invention will be further explained below in conjunction with specific embodiments.

[0018] The microfluidizer used in the examples of the present invention was purchased from Suzhou Microfluidic Nanobiotechnology Co., Ltd., model number NanoGenizer-II; the raw materials were all commercially available.

[0019] The preparation method of hyaluronic acid grafted proanthocyanidin of the present invention is: proanthocyanidin and hyaluronic acid are dispersed in deionized water respectively, laccase is added to the proanthocyanidin solution for incubation, and then the anthocyanidin solution containing laccase and the hyaluronic acid solution are mixed for reaction, and the reaction product is filtered and purified to obtain hyaluronic acid grafted proanthocyanidin.

[0020] Example 1

[0021] The present invention provides a bionic vernix caseosa liposome, which comprises the following raw materials in parts by weight: 15 parts of hydrogenated lecithin, 10 parts of amino acids, 10 parts of stearic acid, 3 parts of ceramide, 5 parts of tocopherol acetate, 20 parts of squalane, 2 parts of palmitoyl tripeptide-8, 3 parts of plant polysaccharides, 5 parts of vegetable oils, 3 parts of hyaluronic acid grafted proanthocyanidins, and 20 parts of deionized water.

[0022] Among them, ceramide is composed of ceramide EOP, ceramide NP and ceramide AS in a mass ratio of 1:1:1. Plant polysaccharides are composed of Bletilla striata polysaccharide, Dendrobium officinale polysaccharide and Osmanthus fragrans leaf polysaccharide in a mass ratio of 1:1:1. Plant oils are tea oil and shea butter in a mass ratio of 2:1. Amino acids are proline, serine and lysine in a mass ratio of 1:1:1.

[0023] The method for preparing the bionic vernix caseosa liposomes proposed by the present invention comprises the following steps:

[0024] S1: dispersing amino acids, palmitoyl tripeptide-8, hyaluronic acid-grafted proanthocyanidins and plant polysaccharides in deionized water to form an aqueous phase;

[0025] S2: mixing hydrogenated lecithin, stearic acid, ceramide, tocopheryl acetate, squalane and vegetable oil evenly to form an oil phase;

[0026] S3: The water phase of S1 and the oil phase of S2 are homogenized in a microfluidizer to prepare bionic vernix caseosa liposomes.

[0027] The homogenization temperature in S3 is 45°C and the homogenization pressure is 15000psi.

[0028] Example 2

[0029] The present invention provides a bionic vernix caseosa liposome, which comprises the following raw materials in parts by weight: 10 parts of hydrogenated lecithin, 5 parts of amino acids, 5 parts of stearic acid, 1 part of ceramide, 1 part of tocopherol acetate, 15 parts of squalane, 1 part of palmitoyl tripeptide-8, 1 part of plant polysaccharide, 1 part of vegetable oil, 1 part of hyaluronic acid grafted proanthocyanidin, and 10 parts of deionized water.

[0030] The ceramide is composed of ceramide EOP, ceramide NP and ceramide AS in a mass ratio of 2:1:1. The plant polysaccharide is composed of bletilla striata polysaccharide, dendrobium officinale polysaccharide and osmanthus leaf polysaccharide in a mass ratio of 3:1:1. The plant oil is olive oil. The amino acid is proline.

[0031] The method for preparing the bionic vernix caseosa liposomes proposed by the present invention comprises the following steps:

[0032] S1: dispersing amino acids, palmitoyl tripeptide-8, hyaluronic acid-grafted proanthocyanidins and plant polysaccharides in deionized water to form an aqueous phase;

[0033] S2: mixing hydrogenated lecithin, stearic acid, ceramide, tocopheryl acetate, squalane and vegetable oil evenly to form an oil phase;

[0034] S3: The water phase of S1 and the oil phase of S2 are homogenized in a microfluidizer to prepare bionic vernix caseosa liposomes.

[0035] The homogenization temperature in S3 is 30°C and the homogenization pressure is 10,000 psi.

[0036] Example 3

[0037] The present invention provides a bionic vernix caseosa liposome, which comprises the following raw materials in parts by weight: 20 parts of hydrogenated lecithin, 15 parts of amino acids, 15 parts of stearic acid, 5 parts of ceramide, 10 parts of tocopherol acetate, 25 parts of squalane, 3 parts of palmitoyl tripeptide-8, 5 parts of plant polysaccharides, 10 parts of vegetable oil, 5 parts of hyaluronic acid grafted proanthocyanidins, and 30 parts of deionized water.

[0038] The ceramide is composed of ceramide EOP, ceramide NP and ceramide AS in a mass ratio of 1:2:2. The plant polysaccharide is composed of Bletilla striata polysaccharide, Dendrobium officinale polysaccharide and Osmanthus fragrans leaf polysaccharide in a mass ratio of 1:3:3. The plant oil is tea oil, coconut oil and olive oil in a mass ratio of 1:1:1. The amino acid is glutamic acid.

[0039] The method for preparing the bionic vernix caseosa liposomes proposed by the present invention comprises the following steps:

[0040] S1: dispersing amino acids, palmitoyl tripeptide-8, hyaluronic acid-grafted proanthocyanidins and plant polysaccharides in deionized water to form an aqueous phase;

[0041] S2: mixing hydrogenated lecithin, stearic acid, ceramide, tocopheryl acetate, squalane and vegetable oil evenly to form an oil phase;

[0042] S3: The water phase of S1 and the oil phase of S2 are homogenized in a microfluidizer to prepare bionic vernix caseosa liposomes.

[0043] The homogenization temperature in S3 is 60°C and the homogenization pressure is 20,000 psi.

[0044] Comparative Example 1

[0045] The present invention provides a bionic vernix caseosa liposome, which comprises the following raw materials in parts by weight: 15 parts of hydrogenated lecithin, 10 parts of amino acids, 10 parts of stearic acid, 3 parts of ceramide, 5 parts of tocopherol acetate, 20 parts of squalane, 2 parts of palmitoyl tripeptide-8, 3 parts of plant polysaccharides, 5 parts of vegetable oils, 3 parts of hyaluronic acid grafted proanthocyanidins, and 20 parts of deionized water.

[0046] The ceramide is ceramide EOP. The plant polysaccharide is composed of Bletilla striata polysaccharide, Dendrobium officinale polysaccharide and Osmanthus fragrans leaf polysaccharide in a mass ratio of 1:1:1. The plant oil is tea oil and shea butter in a mass ratio of 2:1. The amino acids are proline, serine and lysine in a mass ratio of 1:1:1.

[0047] The preparation method of the above-mentioned bionic vernix caseosa liposome is the same as that in Example 1.

[0048] Comparative Example 2

[0049] The present invention provides a bionic vernix caseosa liposome, which comprises the following raw materials in parts by weight: 15 parts of hydrogenated lecithin, 10 parts of amino acids, 10 parts of stearic acid, 3 parts of ceramide, 5 parts of tocopherol acetate, 20 parts of squalane, 2 parts of palmitoyl tripeptide-8, 3 parts of plant polysaccharides, 5 parts of vegetable oils, 3 parts of hyaluronic acid grafted proanthocyanidins, and 20 parts of deionized water.

[0050] The ceramide is ceramide NP. The plant polysaccharide is composed of Bletilla striata polysaccharide, Dendrobium officinale polysaccharide and Osmanthus fragrans leaf polysaccharide in a mass ratio of 1:1:1. The plant oil is tea oil and shea butter in a mass ratio of 2:1. The amino acids are proline, serine and lysine in a mass ratio of 1:1:1.

[0051] The preparation method of the above-mentioned bionic vernix caseosa liposome is the same as that in Example 1.

[0052] Comparative Example 3

[0053] The present invention provides a bionic vernix caseosa liposome, which comprises the following raw materials in parts by weight: 15 parts of hydrogenated lecithin, 10 parts of amino acids, 10 parts of stearic acid, 3 parts of ceramide, 5 parts of tocopherol acetate, 20 parts of squalane, 2 parts of palmitoyl tripeptide-8, 3 parts of plant polysaccharides, 5 parts of vegetable oils, 3 parts of hyaluronic acid grafted proanthocyanidins, and 20 parts of deionized water.

[0054] The ceramide is ceramide AS. The plant polysaccharide is composed of Bletilla striata polysaccharide, Dendrobium officinale polysaccharide and Osmanthus fragrans leaf polysaccharide in a mass ratio of 1:1:1. The plant oil is tea oil and shea butter in a mass ratio of 2:1. The amino acids are proline, serine and lysine in a mass ratio of 1:1:1.

[0055] The preparation method of the above-mentioned bionic vernix caseosa liposome is the same as that in Example 1.

[0056] Comparative Example 4

[0057] The present invention provides a bionic vernix caseosa liposome, which comprises the following raw materials in parts by weight: 15 parts of hydrogenated lecithin, 10 parts of amino acids, 10 parts of stearic acid, 3 parts of ceramide, 5 parts of tocopherol acetate, 20 parts of squalane, 2 parts of palmitoyl tripeptide-8, 3 parts of plant polysaccharides, 5 parts of vegetable oils, 3 parts of hyaluronic acid grafted proanthocyanidins, and 20 parts of deionized water.

[0058] The ceramide is composed of ceramide EOP, ceramide NP and ceramide AS in a mass ratio of 1:1:1. The plant polysaccharide is bletilla striata polysaccharide. The plant oil is tea oil and shea butter in a mass ratio of 2:1. The amino acids are proline, serine and lysine in a mass ratio of 1:1:1.

[0059] The preparation method of the above-mentioned bionic vernix caseosa liposome is the same as that in Example 1.

[0060] Comparative Example 5

[0061] The present invention provides a bionic vernix caseosa liposome, which comprises the following raw materials in parts by weight: 15 parts of hydrogenated lecithin, 10 parts of amino acids, 10 parts of stearic acid, 3 parts of ceramide, 5 parts of tocopherol acetate, 20 parts of squalane, 2 parts of palmitoyl tripeptide-8, 3 parts of plant polysaccharides, 5 parts of vegetable oils, 3 parts of hyaluronic acid grafted proanthocyanidins, and 20 parts of deionized water.

[0062] The ceramide is composed of ceramide EOP, ceramide NP and ceramide AS in a mass ratio of 1:1:1. The plant polysaccharide is Dendrobium officinale polysaccharide. The plant oil is tea oil and shea butter in a mass ratio of 2:1. The amino acids are proline, serine and lysine in a mass ratio of 1:1:1.

[0063] The preparation method of the above-mentioned bionic vernix caseosa liposome is the same as that in Example 1.

[0064] Comparative Example 6

[0065] The present invention provides a bionic vernix caseosa liposome, which comprises the following raw materials in parts by weight: 15 parts of hydrogenated lecithin, 10 parts of amino acids, 10 parts of stearic acid, 3 parts of ceramide, 5 parts of tocopherol acetate, 20 parts of squalane, 2 parts of palmitoyl tripeptide-8, 3 parts of plant polysaccharides, 5 parts of vegetable oils, 3 parts of hyaluronic acid grafted proanthocyanidins, and 20 parts of deionized water.

[0066] The ceramide is composed of ceramide EOP, ceramide NP and ceramide AS in a mass ratio of 1:1:1. The plant polysaccharide is osmanthus leaf polysaccharide. The plant oil is tea oil and shea butter in a mass ratio of 2:1. The amino acids are proline, serine and lysine in a mass ratio of 1:1:1.

[0067] The preparation method of the above-mentioned bionic vernix caseosa liposome is the same as that in Example 1.

[0068] Comparative Example 7

[0069] The present invention provides a bionic vernix caseosa liposome, which comprises the following raw materials in parts by weight: 15 parts of hydrogenated lecithin, 10 parts of amino acids, 10 parts of stearic acid, 3 parts of ceramide, 5 parts of tocopherol acetate, 20 parts of squalane, 2 parts of palmitoyl tripeptide-8, 3 parts of plant polysaccharides, 5 parts of vegetable oils, 3 parts of hyaluronic acid grafted proanthocyanidins, and 20 parts of deionized water.

[0070] Among them, ceramide is composed of ceramide EOP, ceramide NP and ceramide AS in a mass ratio of 1:1:1. Plant polysaccharides are composed of Bletilla striata polysaccharide, Dendrobium officinale polysaccharide and Osmanthus fragrans leaf polysaccharide in a mass ratio of 1:1:1. Plant oils are tea oil and shea butter in a mass ratio of 2:1. Amino acids are proline, serine and lysine in a mass ratio of 1:1:1.

[0071] The preparation method of the above-mentioned bionic vernix caseosa liposome is the same as that in Example 1.

[0072] The moisturizing properties and encapsulation efficiency of the bionic vernix caseosa liposomes prepared in Examples 1-3 and Comparative Examples 1-7 were measured. The test results are shown in Table 1.

[0073] The moisturizing performance test method is as follows: the subjects were randomly divided into 9 groups, with 6 people in each group, half of whom were male and half were female, aged between 20 and 30 years old. The 4cm*4cm range of the forearm was used as the test area, and the sample to be tested was applied to the test area. The skin moisture was tested by the skin moisture tester CK-MPA10 before using the liposomes, 1h, 3h and 5h after using the liposomes, and the average value of each group was taken.

[0074] Encapsulation efficiency test method: After ultrafiltration of the liposomes, the amino acid content in the supernatant is determined by high performance liquid chromatography, which is the free amino acid content; after the liposomes are broken by anhydrous ethanol, the amino acid content is determined by high performance liquid chromatography, which is the total amino acid content, and then the encapsulation efficiency is calculated by the free amino acid content and the total amino acid content.

[0075] Table 1 Moisturizing performance test results

[0076]

[0077]

[0078] It can be seen from the test results of Examples 1-3 in Table 1 that the liposomes of the bionic vernix caseosa of the present invention are composed of hydrogenated lecithin, amino acids, stearic acid, ceramide, tocopherol acetate, squalane, palmitoyl tripeptide-8, plant polysaccharides and plant oils, and can be combined with the natural stratum corneum of the skin to enhance the integrity of the skin barrier, and the liposomes of the bionic vernix caseosa can form a protective film on the skin surface, reduce the evaporation of water on the skin surface, and lock in water at the same time. This protective film has a strong affinity with the skin and is not easily destroyed by the external environment, providing a long-lasting moisturizing effect. It can be seen from the test results of Example 1 and Comparative Examples 1-3 that the ceramide of the present invention is composed of ceramide EOP, ceramide NP and ceramide AS, which can further improve the moisturizing performance of the liposome compared to the use of a single type of ceramide. It can be seen from the test results of Example 1 and Comparative Examples 4-6 that when the plant polysaccharide is composed of Bletilla striata polysaccharide, Dendrobium officinale polysaccharide and Osmanthus fragrans leaf polysaccharide, it has a better moisturizing performance than using a single component, indicating that when these three polysaccharide components are applied to the liposomes of the present application, they have a synergistic effect in improving the moisturizing performance of the liposomes. This is because the combined effect of Bletilla striata polysaccharide, Dendrobium officinale polysaccharide and Osmanthus fragrans leaf polysaccharide can significantly improve the hydration of the skin, maintain the moisture balance of the skin, and help to enhance the skin barrier function and reduce water loss. It can be seen from the test results of Example 1 and Comparative Example 7 that the hyaluronic acid grafted proanthocyanidin of the present invention not only has a certain moisturizing performance, but also can significantly improve the encapsulation rate of the liposome, because the hyaluronic acid grafted proanthocyanidin can improve the stability of the liposome through hydrogen bonding and electrostatic interaction, thereby improving the encapsulation rate of the liposome after homogenization by a microfluidizer.

Claims

1. A bionic vernix caseosa liposome, characterized in that: The invention comprises the following raw materials in parts by weight: 10-20 parts of hydrogenated lecithin, 5-15 parts of amino acids, 5-15 parts of stearic acid, 1-5 parts of ceramide, 1-10 parts of tocopheryl acetate, 15-25 parts of squalane, 3 parts of palmitoyl tripeptide-81, 1-5 parts of plant polysaccharides, 1-10 parts of vegetable oil, 1-5 parts of hyaluronic acid grafted proanthocyanidins, and 10-30 parts of deionized water; The plant polysaccharide is composed of bletilla striata polysaccharide, dendrobium officinale polysaccharide and osmanthus leaf polysaccharide in a mass ratio of 3:1-9:1-9.

2. The bionic vernix caseosa liposome according to claim 1, characterized in that: The ceramide is composed of ceramide EOP, ceramide NP and ceramide AS in a mass ratio of 1:0.5-2:0.5-2.

3. The bionic vernix caseosa liposome according to claim 1, characterized in that: The vegetable oil is one or more of tea oil, coconut oil, olive oil and shea butter.

4. The bionic vernix caseosa liposome according to claim 1, characterized in that: The amino acid is one or more of glycine, glutamic acid, aspartic acid, alanine, proline, serine, lysine, tryptophan and cysteine.

5. The method for preparing the bionic vernix caseosa liposome according to any one of claims 1 to 4, characterized in that: The steps are as follows: S1: dispersing amino acids, palmitoyl tripeptide-8, hyaluronic acid-grafted proanthocyanidins and plant polysaccharides in deionized water to form an aqueous phase; S2: mixing hydrogenated lecithin, stearic acid, ceramide, tocopheryl acetate, squalane and vegetable oil evenly to form an oil phase; S3: The water phase of S1 and the oil phase of S2 are homogenized in a microfluidizer to prepare bionic vernix caseosa liposomes.

6. The method for preparing biomimetic vernix caseosa liposomes according to claim 5, characterized in that: The homogenization temperature in S3 is 30-60°C, and the homogenization pressure is 10000-20000psi.

7. Use of the bionic vernix caseosa liposome as claimed in any one of claims 1 to 4 in skin care products.

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