Ganoderma lucidum extract dressing and application thereof in promoting skin wound repair

Abstract

The invention discloses a ganoderma lucidum extract dressing. The ganoderma lucidum extract dressing comprises ganoderma lucidum source chitosan, ganoderma lucidum polysaccharide, ganoderma lucidum source carboxymethyl chitosan and carbomer. The invention further discloses application of the lucid ganoderma extract dressing in promoting skin wound repair and preparing products for promoting skin wound repair. The invention further discloses a preparation method of the lucid ganoderma extract dressing. The lucid ganoderma extract dressing disclosed by the invention has antibacterial and hemostatic functions, can effectively avoid the situation that common chitosan generates a sensitization effect, accelerates the wound healing and repairing process, and effectively improves the wound healing situation.

Description

Technical Field

[0001] The invention belongs to the field of biological dressings, and in particular relates to a ganoderma lucidum extract dressing and application thereof in promoting skin wound repair. Background Art

[0002] Dressings are medical materials that can temporarily protect wounds, prevent infection, and promote healing. According to different materials, dressings can be divided into traditional dressings (such as absorbent cotton gauze), biological dressings, and synthetic dressings. With the continuous improvement of modern medical standards, traditional dressings can no longer meet people's requirements for different types of wound repair. Traditional dressings such as cotton gauze are widely available, inexpensive, and have good fluid absorption capacity, but they are easy to adhere to wounds, do not isolate bacteria, are easy to dry, lack biological activity, have limited promotion of wound healing, and may cause allergic reactions.

[0003] Biological dressings are new wound repair and protection materials developed on the basis of the "moist healing" theory of wound repair. Biological dressings can be divided into two categories: artificial biological dressings and allogeneic tissue wound coverings. Common artificial biological dressings are made of materials such as collagen, chitosan, and hyaluronic acid. They have hemostatic and coagulation effects and can induce the proliferation and differentiation of various cells. Compared with traditional dressings, biological dressings have the advantages of reducing infection, improving wound healing quality, alleviating patient pain, and facilitating the operation of medical staff, so they are favored by people.

[0004] There are many kinds of biological dressings on the market, each with its own advantages and disadvantages. Most of them have disadvantages such as easy to cause secondary infection, easy to adhere to wounds and poor hemostasis. For example, collagen dressings can easily absorb exudate, promote granulation growth, and have good biocompatibility, but they need to be changed frequently and are not suitable for infected wounds. Chitosan dressings have good biocompatibility, good moisture permeability and air permeability, can accelerate wound healing, and have certain antibacterial, hemostatic and wound healing abilities, but their immunoregulatory and anti-allergic properties are weak, and their functions are relatively single, and they fail to fully utilize the multiple biological activities of chitosan. In addition, ordinary chitosan is extracted from shrimp and crab shells, contains allergenic proteins, and is prone to allergic adverse reactions. Alginate dressings have strong water absorption capacity, certain biological activity, can effectively repair wounds, and have good biocompatibility, but may cause wound dehydration, and have no adhesion. Hyaluronic acid dressings have strong moisturizing ability and repair effects, but they have no antibacterial properties and are not suitable for wound infections. No existing dressing has been found to have the functions of antibacterial, promoting wound healing, and providing trace elements and nutrition at the same time.

[0005] Lingzhi Ganoderma lucidum(Curtis) P.Karst.) is regarded as a precious tonic herb by traditional Chinese medicine, which has the effects of replenishing qi and calming the mind, strengthening the body and consolidating the foundation. Ganoderma lucidum spores are extremely tiny oval reproductive cells ejected from the gills of Ganoderma lucidum during the growth and maturity period. They are rich in Ganoderma lucidum triterpenes, polysaccharides, sterols, fatty acids, nucleosides and other ingredients. Ganoderma lucidum (spores) play the main functional ingredients of anti-inflammatory, analgesic, sedative, anti-aging, tumor cell killing, anti-hypoxia and other effects. The specific pharmacological effects include anti-tumor, anti-oxidation, immunomodulation, cardiovascular protection, liver protection, protection of the nervous system, etc. Summary of the invention

[0006] The purpose of the present invention is to provide an improved biological dressing that has the functions of antibacterial, hemostatic and wound healing promotion in view of the above technical problems to be solved.

[0007] Another object of the present invention is to provide application of the biological dressing.

[0008] Another object of the present invention is to provide a method for preparing the biological dressing.

[0009] In order to achieve the above objectives, the present invention provides the following technical solutions.

[0010] In one aspect, the present invention provides a ganoderma lucidum extract dressing, which comprises ganoderma lucidum-derived chitosan, ganoderma lucidum polysaccharide, ganoderma lucidum-derived carboxymethyl chitosan and carbomer.

[0011] Preferably, the weight ratio of ganoderma lucidum-derived chitosan, ganoderma lucidum polysaccharide, ganoderma lucidum-derived carboxymethyl chitosan and carbomer is 3:6:3:1.

[0012] Preferably, the total weight of ganoderma-derived chitosan, ganoderma-derived polysaccharide, ganoderma-derived carboxymethyl chitosan and carbomer accounts for 6.5% of the weight of the composition.

[0013] Preferably, the ganoderma lucidum-derived chitosan is prepared by the following steps: (1) Ganoderma lucidum spore powder was added to deionized water to prepare a 5 w / v% mixture, and the mixture was ultrasonically treated at 70°C for 50 minutes, followed by centrifugation at 12,000 rpm for 15 minutes, the supernatant was removed, deionized water was added again to make up the primary volume, and then ultrasonicated and centrifuged, and the process was repeated three times; (2) drying the Ganoderma lucidum spore powder precipitate after removing the supernatant at 65° C. and about -0.095 MPa for 3 days, then bleaching it with hydrogen peroxide at 70° C. for 2 hours, adjusting the pH to neutral with a 10 w / v% NaOH solution, and then centrifuging it at 7000 rpm and room temperature for 10 minutes, collecting the precipitate and drying it at 65° C. and about -0.095 MPa for 3 days to obtain a Ganoderma lucidum spore powder residue; (3) Take a portion of Ganoderma lucidum spore powder residue, add a 20 w / v% NaOH solution at a solid-liquid ratio of 1:15, mix well and perform ultrasonic treatment at room temperature. After the ultrasonic treatment, centrifuge at room temperature and 7000 rpm for 10 minutes, collect the precipitate, and dry it at 65°C and about -0.095 MPa for 3 days to obtain Ganoderma lucidum source chitosan.

[0014] Preferably, the ganoderma lucidum polysaccharide is prepared by the following steps: Ganoderma lucidum spore powder was added to deionized water to prepare a 5 w / v% mixture, which was ultrasonically treated at 70°C for 50 minutes, and then centrifuged at 12000 rpm for 15 minutes, the supernatant was collected, and deionized water was added again to make up the primary volume, followed by ultrasonication and centrifugation. The process was repeated three times, and all the collected supernatants were rotary distilled together at 50°C, and the obtained sample was freeze-dried to obtain Ganoderma lucidum polysaccharide.

[0015] In the above step (1) of preparing chitosan from Ganoderma lucidum, all the collected supernatant is placed in a flask, and then Preferably, the ganoderma lucidum derived carboxymethyl chitosan is prepared by the following steps: 3.0 g of Ganoderma lucidum chitosan powder was added to 70.8 mL of isopropanol and magnetically stirred for 30 minutes to form a suspension. 8.16 g of sodium hydroxide and 10 mL of isopropanol dissolved in 10 g of deionized water were added to the suspension and stirred for 1 hour. Then, 14.4 g of monochloroacetic acid / isopropanol solution was added and magnetically stirred for 4 hours. 100 mL of ethanol was added to terminate the reaction. Then, the mixed reaction liquid was filtered, the solid was washed with 70% to 90% v / v ethanol and dried at room temperature. 1.0 g of the dry powder obtained above was suspended in 100 mL of 80% v / v ethanol / water solution, 1 mL of 37% hydrochloric acid was added and stirred for 30 minutes to achieve neutralization. The suspension was filtered, the solid was fully washed with 70% to 90% v / v ethanol, and vacuum dried to obtain Ganoderma lucidum carboxymethyl chitosan.

[0016] Preferably, the carbomer is carbomer 980.

[0017] Preferably, the ganoderma lucidum extract dressing comprises, by weight, 1.5% ganoderma lucidum-derived chitosan, 3% ganoderma lucidum polysaccharide, 1.5% ganoderma lucidum-derived carboxymethyl chitosan and 0.5% carbomer.

[0018] More preferably, the ganoderma lucidum extract dressing of the present invention further comprises a composite amino acid nutrient component, wherein the composite amino acid nutrient component preferably comprises glutamic acid, arginine, leucine, tryptophan, glycine, valine and adenine.

[0019] More preferably, the Ganoderma lucidum extract dressing consists of the following components by weight: 1.5% Ganoderma lucidum source chitosan, 3% Ganoderma lucidum polysaccharide, 1.5% Ganoderma lucidum source carboxymethyl chitosan, 0.5% Carbomer 980, 0.03% glutamic acid, 0.04% arginine, 0.05% leucine, 0.033% tryptophan, 0.03% glycine, 0.04% valine, 0.04% adenine, and the balance deionized water.

[0020] On the other hand, the present invention also provides the use of the ganoderma lucidum extract dressing in promoting skin wound repair.

[0021] On the other hand, the present invention also provides the use of the ganoderma lucidum extract dressing in the preparation of a product for promoting skin wound repair.

[0022] On the other hand, the present invention also provides a method for preparing the ganoderma lucidum extract dressing, which comprises the following steps: mixing and stirring the raw materials of each component until they are uniformly dispersed. Preferably, stirring is performed under vacuum at a stirring speed of 700 rpm.

[0023] On the other hand, the present invention also provides a skin wound repair product, which comprises the ganoderma lucidum extract dressing and fabric of the present invention. The fabric can be a medical non-woven fabric.

[0024] The salt formed by Ganoderma lucidum chitosan and acid can cause red blood cells to adhere, aggregate and deform, and finally form a firm blood clot to block the bleeding point. This pathway does not rely on the classical coagulation cascade reaction, which is the biggest feature of Ganoderma lucidum chitosan that is different from other hemostatic agents. In simple terms, Ganoderma lucidum chitosan exhibits cationic properties under acidic conditions, which can aggregate negatively charged platelets, thereby achieving a good hemostatic effect. It also has an antibacterial effect and can promote wound healing. Ganoderma lucidum polysaccharides have moisturizing and repairing functions, can provide a moisturizing environment, lower blood sugar, lower blood lipids, resist thrombosis, resist oxidation, scavenge free radicals, resist aging, resist radiation, resist tumors, promote blood circulation, regulate immunity, regulate nucleic acid and protein metabolism, promote DNA synthesis, and promote the proliferation of human umbilical cord blood LAK cells. Ganoderma lucidum carboxymethyl chitosan has the effects of hemostasis, antibacterial, analgesia, hemostasis, and inhibition of scar hyperplasia. Carbomer 980 can improve the film-forming property of skin wound repair dressings, isolate the wound from the outside world, keep it moist and not easily condense into a scab, and avoid adhesion to the covered tissue wound. Carbomer contains relatively long side chains, has good flexibility, excellent transparency, strong adhesion, good light resistance, heat resistance, cold resistance, water resistance, film-forming property, miscibility, and also has high tensile strength and impact resistance.

[0025] Compared with the prior art, the ganoderma extract dressing of the present invention produces a synergistic effect by compounding a variety of ganoderma extracts such as ganoderma-derived chitosan, ganoderma polysaccharide and ganoderma-derived carboxymethyl chitosan in a suitable proportion, and has bactericidal and antibacterial functions. In addition, the ganoderma extract dressing of the present invention can also effectively avoid the sensitization effect of ordinary chitosan (extracted from shrimp and crab shells). The ganoderma extract dressing of the present invention can promote the growth of fibroblasts and epidermal keratinocytes, promote fibroblast proliferation and improve the phagocytic ability of macrophages due to its immunomodulatory pharmacological effect, and can also accelerate the wound healing and repair process, effectively improving the wound healing condition. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 The results of antibacterial experiments are shown.

[0027] Figure 2 Cell survival assay results are shown.

[0028] Figure 3 The results of animal wound repair experiments are shown.

[0029] Figure 4 Results of cell staining experiments are shown.

[0030] Figure 5 The results of the hemostasis experiment are shown. DETAILED DESCRIPTION

[0031] The present invention will be further described below in conjunction with specific examples. It should be understood that the following examples are only used to illustrate the present invention, rather than to limit the scope of the present invention.

[0032] Example 1 The components of the Ganoderma lucidum extract dressing composition are as follows: Ganoderma lucidum chitosan 1.5%, Ganoderma lucidum polysaccharide 3%, Ganoderma lucidum carboxymethyl chitosan 1.5%, Carbomer 980 0.5%, Glutamic acid 0.03%, Arginine 0.04%, Leucine 0.05%, Tryptophan 0.033%, Glycine 0.03%, Valine 0.04%, Adenine 0.04%, Deionized water makes up to 100%. The above percentages are based on the total weight of the composition.

[0033] The ganoderma lucidum extract dressing composition was prepared according to the following steps: 1. Preparation of Ganoderma lucidum chitosan (1) Add Ganoderma lucidum spore powder to deionized water to prepare a 5 w / v% mixture. Use a KS-300EI ultrasonic device to ultrasonicate the mixture (frequency 40 kHz) at 70°C for 50 minutes, then centrifuge at 12,000 rpm for 15 minutes, and remove and collect the supernatant. Add deionized water again (to make up the first-level volume), then ultrasonicate (frequency 40 kHz) and centrifuge (12,000 rpm, 15 minutes), and repeat this process three times. Place all the collected supernatant in a flask and set aside for the preparation of Ganoderma lucidum polysaccharide.

[0034] (2) The Ganoderma lucidum spore powder precipitate after the removal of the supernatant was dried in a vacuum drying oven at 65°C and about -0.095MPa for 3 days. Then, it was bleached with hydrogen peroxide at 70°C for 2 hours, and then adjusted to a neutral pH (pH≈7.0) with a 10w / v% NaOH solution. The precipitate was centrifuged at 7000rpm and room temperature for 10 minutes, and the precipitate was collected and dried at 65°C and about -0.095MPa for 3 days to obtain a Ganoderma lucidum spore powder residue.

[0035] (3) Take a portion of Ganoderma lucidum spore powder residue (about 0.5g), add a NaOH solution with a concentration of 20w / v% according to a solid-liquid ratio of 1:15 (g:ml), mix well, and apply ultrasound (frequency 40 kHz) at room temperature (about 25℃) for 2 hours. During the ultrasonic treatment, the volume of deionized water in the container is about 2L. The distance between the ultrasonic generator probe and the sample (>2cm) remains unchanged. The temperature of the deionized water and the ultrasonic generator is recorded every 5 minutes. After the ultrasonic treatment, centrifuge at room temperature and 7000rpm for 10 minutes, collect the precipitate, and dry it at 65℃ and about -0.095MPa for 3 days to obtain Ganoderma lucidum source chitosan.

[0036] 2. Preparation of Ganoderma lucidum polysaccharide In the above step (1) of preparing Ganoderma lucidum-derived chitosan, all the collected supernatants are placed in a flask and then subjected to rotary distillation at 50° C. The obtained sample is freeze-dried to obtain Ganoderma lucidum polysaccharide.

[0037] 3. Preparation of Ganoderma lucidum carboxymethyl chitosan 3.0 g of Ganoderma lucidum chitosan powder (obtained by the above-mentioned step of "Preparing Ganoderma lucidum chitosan") was added to 70.8 mL of isopropanol and kept under magnetic stirring for 30 minutes to form a suspension. Then, 8.16 g of sodium hydroxide and 10 mL of isopropanol dissolved in 10 g of deionized water were added to the suspension and stirred for 1 hour. Then, 14.4 g of monochloroacetic acid / isopropanol solution (monochloroacetic acid to isopropanol molar ratio 1:1) was added, and the reaction was carried out under magnetic stirring for 4 hours, and the reaction was stopped by adding 100 mL of ethanol. Then, the mixed reaction solution was filtered and the solid was washed with 70% to 90% (preferably 80%) v / v ethanol (the ethanol content was increased from 70% to 90% by volume) and dried at room temperature. 1.0 g of the dry powder obtained above was suspended in 80% v / v ethanol / water solution (100 mL), hydrochloric acid (1 mL, volume concentration 37%) was added and stirred for 30 minutes to achieve neutralization. Finally, the suspension is filtered, the solid is fully washed with 70% to 90% (preferably 80%) v / v ethanol, and vacuum dried to obtain Ganoderma lucidum-derived carboxymethyl chitosan.

[0038] 4. Clean the reactor, containers, valves, cutter heads and other related equipment with purified water above 80°C, and then rinse with 75% v / v disinfectant alcohol.

[0039] 5. Pour deionized water into the reaction vessel, add Carbomer 980, start stirring and dispersing, use radial flow dissolving stirring paddles to quickly disperse and dissolve the material to ensure that the sample on the wall and bottom of the kettle is in a flowing state and brought to the center of the kettle to avoid agglomeration. Control the stirring speed to 700 rpm and evacuate.

[0040] 6. Heat to 80℃, use a disperser (homogenizer) to homogenize for 3 minutes to make the material more delicate and avoid bubbles. Sterilize and keep warm for 30 minutes, and start cooling after passing the test (cooling while stirring).

[0041] 7. When the temperature drops to 45°C, add the remaining ingredients in turn and stir evenly. Stir for 15 minutes until fully mixed.

[0042] 8. Take samples for testing, filter out the materials after they are qualified, and obtain the finished product.

[0043] Product effect testing The ganoderma lucidum extract dressing obtained in Example 1 was tested to evaluate its product application effect.

[0044] 1. Antibacterial experiment Solid culture medium: Add 2.1 g LB broth and 1.5 g agar powder to 100 mL ultrapure water, sterilize in an autoclave for 20 minutes, and cool naturally to 60°C for later use.

[0045] Liquid culture medium: Add 2.1 g LB broth to 100 mL ultrapure water, sterilize it in an autoclave for 20 minutes, and cool it naturally to 60°C before use.

[0046] With Escherichia coli ( E. coil , Gram-negative rods) and Staphylococcus aureus ( S. aureus , Gram-positive cocci) to study the antibacterial properties of the dressing. 0.5 mL of Escherichia coli and Staphylococcus aureus stock solution was added to 5 mL of sterilized liquid culture medium, which was then placed in a constant temperature shaker for 12-28 hours. The bacterial solution was diluted to 1×10 5 CFU / mL, add 50 μL dressing and culture in a 37°C incubator for 12 hours, then dilute the bacterial suspension by 10 5 Times, take 100 μL of the diluted suspension and evenly spread it on the solid culture medium to observe the antibacterial effect of the dressing. No dressing was added to the blank control group.

[0047] The results of antibacterial experiments are as follows Figure 1 As shown in the figure, compared with the blank control group (Con group), the Ganoderma lucidum extract dressing of Example 1 (CS group) showed good antibacterial properties against both Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria Escherichia coli.

[0048] 2. Cell survival assay The ganoderma lucidum extract dressing of Example 1 of the present invention was soaked in a non-woven fabric (just soak it), and then cut into small discs with a diameter of 1 cm using a hole puncher and placed in a 48-well plate. The prepared mouse L929 cell suspension (1×10 4 The 48-well plate was transferred to a cell culture incubator and the cells were stained with a live / dead cell double staining kit (Yisheng Bio, 40747ES76) to observe the biocompatibility of the Ganoderma lucidum extract dressing. The cell survival rate was calculated by CCK8 assay. No dressing was used in the blank control group.

[0049] The results of cell survival experiments were as follows Figure 2 As shown. Among them, Con is a blank control group, Col is a commercially available collagen dressing group, HA is a hyaluronic acid dressing group, and CS is a Ganoderma lucidum extract dressing group. The live cell / dead cell staining results show that after L929 cells were cultured in a medium containing Ganoderma lucidum extract dressings for 24 hours, there were almost no dead cells (red), and most of the cells were alive (green). The CCK8 results showed that the cell survival rate of the Ganoderma lucidum extract dressing group was above 80%, indicating that the Ganoderma lucidum extract dressing of the present invention has good biocompatibility.

[0050] 3. Animal Wound Repair Experiment Male SD rats weighing 250-300 g were used to evaluate the skin healing performance of the dressings. A 1 cm×1 cm full-thickness skin injury model was constructed on the back of the rats, and commercially available collagen dressings (Col group), hyaluronic acid dressings (HA group) and the ganoderma extract dressings of Example 1 of the present invention (CS group) with an area slightly larger than the wound were placed on the wound for treatment. A natural healing process without a dressing was set as a blank control group (Con group). The dressings on the wound were changed every 3 days, and the wound recovery was recorded by taking photos.

[0051] The healing rate is calculated as follows: Healing rate = (wound area on day 0 - wound area on day n) ÷ wound area on day 0 × 100% The wound skin healing results on days 0, 3, 6, 9, and 12 were as follows Figure 3 shown.

[0052] After 12 days of treatment, the rats were euthanized, and skin sections were prepared and stained with hematoxylin and eosin (H&E) and Masson staining. The sections were observed under a microscope, and the microscopic images of the sections were recorded.

[0053] The results of microscopic observation of sections are as follows Figure 4 As shown, a is the result of H&E staining, and b is the result of Masson staining.

[0054] refer to Figure 3 The results of animal wound repair experiments showed that the Ganoderma lucidum extract dressing of Example 1 of the present invention showed the best repair effect compared with commercially available collagen dressings and hyaluronic acid dressings, with a wound healing rate of about 93.61%. Figure 4 The results of H&E staining and Masson staining showed that the Ganoderma lucidum extract dressing group had more skin appendages, such as blood vessels and hair follicles, compared with other groups, indicating that the skin tissue remodeling process in the Ganoderma lucidum extract dressing group was faster and the healing effect was more significant. Compared with other dressings, Ganoderma lucidum extract dressings performed well in the treatment of chronic difficult-to-heal wounds and burn wounds, and could promote the growth of granulation tissue and the speed of epithelialization, and improve the quality of wound healing.

[0055] 4. Animal wound hemostasis experiment The hemostatic properties of Ganoderma lucidum extract dressings in vivo were studied using a mouse liver injury model. Specifically, the dressing samples were cut into squares of 1 cm × 10 cm in size. 6-8 week-old BALB / c mice were anesthetized and fixed on a surgical cork board, and the abdomen was disinfected with 75% v / v alcohol. The abdominal skin was cut open to expose the anterior part of the liver, and the blood and tissue fluid at the incision site were aspirated with filter paper. Then a pre-weighed filter paper was placed under the liver, and the mass was recorded as W0. A wound was then made in the mouse liver with a sterile needle from a 10 mL syringe. When the blood flowed out of the wound, the dressing was placed on the wound to stop bleeding for 120 seconds, and the filter paper was weighed and recorded as W. At least three parallels were performed for each sample, and the blood loss (in mg) was calculated by the following formula: Blood loss = W-W0 At the same time, the hemostasis conditions were photographed and recorded at 0 seconds, 30 seconds, 60 seconds, 90 seconds and 120 seconds after the dressing started to stop bleeding.

[0056] The results are as follows Figure 5 As shown, a is the observation and record results of hemostasis, and b is the statistical results of blood loss in each group. Figure 5 The figure shows the hemostatic effects of commercially available collagen dressings (Col group), hyaluronic acid dressings (HA group) and ganoderma lucidum extract dressings (CS group), and the natural hemostasis process without the use of dressings is the blank control group (Con group). The results show that compared with the blank control group, the ganoderma lucidum extract dressing group had the least blood loss, and the hemostatic effect of the ganoderma lucidum extract dressing group was significantly better than that of the collagen dressing group and the hyaluronic acid dressing group.

[0057] 5. Safety Assessment The human patch test and biocompatibility test were performed on the Ganoderma lucidum extract dressing of Example 1. The results showed that no adverse reactions and allergic events related to the product occurred, which fully proved that the Ganoderma lucidum extract dressing of the present invention has good biocompatibility and safety.

[0058] In addition, the inventors also prepared a dressing using an equal amount of ordinary chitosan instead of ganoderma lucidum chitosan (other ingredients were the same), and conducted the above-mentioned antibacterial, wound repair, and wound hemostasis experiments. The experimental results showed that the antibacterial, wound repair, and hemostasis effects of the dressing using ordinary chitosan were significantly worse than those of the ganoderma lucidum extract dressing of Example 1.

Claims

1. A ganoderma lucidum extract dressing, characterized in that: The ganoderma lucidum extract dressing comprises ganoderma lucidum-derived chitosan, ganoderma lucidum polysaccharide, ganoderma lucidum-derived carboxymethyl chitosan and carbomer.

2. The ganoderma lucidum extract dressing according to claim 1, characterized in that: The weight ratio of ganoderma lucidum chitosan, ganoderma lucidum polysaccharide, ganoderma lucidum carboxymethyl chitosan and carbomer is 3:6:3:

1.

3. The ganoderma lucidum extract dressing according to claim 1 or 2, characterized in that: The total weight of ganoderma lucidum-derived chitosan, ganoderma lucidum polysaccharide, ganoderma lucidum-derived carboxymethyl chitosan and carbomer accounts for 6.5% of the weight of the composition.

4. The ganoderma lucidum extract dressing according to claim 1, characterized in that: The ganoderma lucidum source chitosan is prepared by the following steps: (1) Ganoderma lucidum spore powder was added to deionized water to prepare a 5 w / v% mixture, and the mixture was ultrasonically treated at 70°C for 50 minutes, followed by centrifugation at 12,000 rpm for 15 minutes, the supernatant was removed, deionized water was added again to make up the primary volume, and then ultrasonicated and centrifuged, and the process was repeated three times; (2) drying the Ganoderma lucidum spore powder precipitate after removing the supernatant at 65° C. and about -0.095 MPa for 3 days, then bleaching it with hydrogen peroxide at 70° C. for 2 hours, adjusting the pH to neutral with a 10 w / v% NaOH solution, and then centrifuging it at 7000 rpm and room temperature for 10 minutes, collecting the precipitate and drying it at 65° C. and about -0.095 MPa for 3 days to obtain a Ganoderma lucidum spore powder residue; (3) Take a portion of Ganoderma lucidum spore powder residue, add a 20 w / v% NaOH solution at a solid-liquid ratio of 1:15, mix well and perform ultrasonic treatment at room temperature. After the ultrasonic treatment, centrifuge at room temperature and 7000 rpm for 10 minutes, collect the precipitate, and dry it at 65°C and about -0.095 MPa for 3 days to obtain Ganoderma lucidum source chitosan.

5. The ganoderma lucidum extract dressing according to claim 1, characterized in that: The ganoderma lucidum polysaccharide is prepared by the following steps: Ganoderma lucidum spore powder was added to deionized water to prepare a 5 w / v% mixture, which was ultrasonically treated at 70°C for 50 minutes, and then centrifuged at 12000 rpm for 15 minutes, the supernatant was collected, and deionized water was added again to make up the primary volume, followed by ultrasonication and centrifugation. The process was repeated three times, and all the collected supernatants were rotary distilled together at 50°C, and the obtained sample was freeze-dried to obtain Ganoderma lucidum polysaccharide.

6. The ganoderma lucidum extract dressing according to claim 1, characterized in that: The ganoderma lucidum-derived carboxymethyl chitosan is prepared by the following steps: 3.0 g of Ganoderma lucidum chitosan powder was added to 70.8 mL of isopropanol and magnetically stirred for 30 minutes to form a suspension. 8.16 g of sodium hydroxide and 10 mL of isopropanol dissolved in 10 g of deionized water were added to the suspension and stirred for 1 hour. Then, 14.4 g of monochloroacetic acid / isopropanol solution was added and magnetically stirred for 4 hours. 100 mL of ethanol was added to terminate the reaction. Then, the mixed reaction liquid was filtered, the solid was washed with 70% to 90% v / v ethanol and dried at room temperature. 1.0 g of the dry powder obtained above was suspended in 100 mL of 80% v / v ethanol / water solution, 1 mL of 37% hydrochloric acid was added and stirred for 30 minutes to achieve neutralization. The suspension was filtered, the solid was fully washed with 70% to 90% v / v ethanol, and vacuum dried to obtain Ganoderma lucidum carboxymethyl chitosan.

7. The ganoderma lucidum extract dressing according to claim 1, characterized in that: The ganoderma lucidum extract dressing comprises, by weight, 1.5% ganoderma lucidum chitosan, 3% ganoderma lucidum polysaccharide, 1.5% ganoderma lucidum carboxymethyl chitosan and 0.5% carbomer.

8. The Ganoderma lucidum extract dressing according to claim 1, characterized in that: The ganoderma extract dressing consists of the following components by weight: 1.5% ganoderma-derived chitosan, 3% ganoderma polysaccharide, 1.5% ganoderma-derived carboxymethyl chitosan, 0.5% carbomer 980, 0.03% glutamic acid, 0.04% arginine, 0.05% leucine, 0.033% tryptophan, 0.03% glycine, 0.04% valine, 0.04% adenine, and the balance deionized water.

9. Use of the ganoderma lucidum extract dressing according to claims 1 to 8 in promoting skin wound repair or in preparing a product promoting skin wound repair.

10. The method for preparing the ganoderma lucidum extract dressing according to claims 1 to 8, the method comprising the following steps: The raw materials of each component are mixed and stirred until they are uniformly dispersed. Preferably, the stirring is carried out under vacuum at a stirring speed of 700 rpm.

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