Combined filler for biological deodorization and preparation method thereof

Abstract

The invention provides a combined filler for biological deodorization and a preparation method thereof, and relates to the technical field of water treatment. The preparation method of the combined filler for biological deodorization, provided by the invention, has the characteristics of simplicity and convenience in operation and stability in operation, the prepared combined filler is high in strength and long in service life, permeation of nutrient substances and proliferation of microorganisms are facilitated, and main malodorous gases such as VOCs, H2S and NH3 in sewage can be quickly and effectively removed through cooperation of the fillers.

Description

Technical Field

[0001] The invention relates to the technical field of water treatment, in particular to a composite filler for biological deodorization and a preparation method thereof. Background Art

[0002] With the progress of society and the improvement of living standards, the public's demand for a better ecological environment is also increasing. The impact of odorous gases produced during sewage treatment on air pollution and the surrounding environment has attracted increasing attention. At present, the main odor pollution control technologies for sewage treatment plants include "washing absorption method, biological method, adsorption method, advanced oxidation method, masking method", etc. Among them, biological deodorization technology has the advantages of low treatment cost, good effect and no secondary pollution. It is the most widely used deodorization technology in sewage treatment plants, but it is easily affected by changes in pollution load and components, non-steady-state conditions and external ambient temperature. There are problems such as unstable treatment effect and low removal rate of pollutants (especially organic pollutants).

[0003] The core of biological deodorization technology is biological fillers and microbial communities. Therefore, improving biological fillers and increasing the abundance and activity of efficient deodorizing microorganisms are the key to the depth and stability of odorous gas removal. Existing deodorizing biological fillers can be roughly divided into three categories: inorganic fillers, organic fillers and composite fillers. Among them, common inorganic fillers include bamboo charcoal, ceramsite and volcanic rock; common organic fillers include artificial synthetic organic materials such as polyolefins and polyurethanes, as well as natural organic materials such as bark, coconut shells and straw; composite fillers are simple mixtures or compression molding of inorganic fillers and organic fillers. Inorganic fillers and artificial synthetic organic materials have the advantages of high strength and long life, but they also have the disadvantages of lack of nutrients required for microbial growth, slow film growth and unstable treatment effect. Natural organic fillers and composite fillers are easy for microorganisms to proliferate, but they have problems such as low strength and short life, and there is also the problem of secondary pollution caused by the corruption of organic matter.

[0004] At present, CN113305731A and CN102910727B provide a method for preparing a deodorizing biological filler by adding nutrients and slow-release agents to the surface during the polishing process of artificially sintered ceramsite. The preparation method proposes that the deodorizing biological filler uses ceramsite materials with high strength and long life as a substrate, and improves the utilization efficiency and cycle of nutrients by adding nutrients and slow-release agents, which is conducive to improving the nutrients required by microorganisms and thus facilitating the proliferation of microorganisms. However, after multiple field practices and theoretical proofs of the above-mentioned technology, it was found that the pore structure of the ceramsite material is not conducive to the penetration of nutrients, resulting in a low nutrient load. In addition, after high-temperature sintering, ceramsite is mostly inert oxides, which are not easily used by microorganisms. In addition, the above-mentioned technology also has the defects of incomplete addition of nutrient elements and no addition of special microorganisms. CN114177767 A provides a method for preparing a biological filler of natural porous ore embedded with Bacillus. However, Bacillus does not have a specific effect on the main malodorous gases such as hydrogen sulfide and ammonia in the odor produced by sewage treatment plants.

[0005] Therefore, it is urgent to provide a new biological deodorizing filler.

[0006] In view of this, the present invention is proposed. Summary of the invention

[0007] The first object of the present invention is to provide a method for preparing a composite filler for biological deodorization to solve the above technical problems.

[0008] The second object of the present invention is to provide a composite filler for biological deodorization.

[0009] In order to achieve the above objectives, the following technical solutions are adopted:

[0010] In a first aspect, the present invention provides a method for preparing a composite filler for biological deodorization, wherein the composite filler comprises a V filler, an H filler, an N filler and a Q filler;

[0011] The bacterial flora loaded on the V filler includes at least one of Pseudomonas phenolvorans, Pseudomonas stutzeri, yeast, Desulfobacillus phenolsulfatus or Alcaligenes;

[0012] The bacterial flora loaded by the H filler includes at least one of Rhodopseudomonas palustris, Chlorobacterium sludgei, Chromobacterium sulphureum or Thiobacillus thiosulfurogen;

[0013] The bacterial flora loaded by the N filler includes nitrifying bacteria;

[0014] The bacterial flora loaded by the Q filler includes at least one of Bacillus, Lactobacillus plantarum, Saccharomyces cerevisiae or Nocardia;

[0015] The preparation method of the V filler, H filler, N filler or Q filler comprises the following steps:

[0016] a. using polyvinyl alcohol as an embedding agent to embed the bacterial suspension to prepare a polyvinyl alcohol-embedded bacterial suspension;

[0017] b. mixing polypropylene, activated carbon and sodium alginate, and then adding a polypropylene foaming agent for foaming to obtain a foamed product;

[0018] c. The foamed product obtained in step b and the polyvinyl alcohol-encapsulated bacterial suspension prepared in step a are mixed, and then pressed and cross-linked in a mold to prepare a V filler, an H filler, an N filler or a Q filler;

[0019] In terms of weight percentage, the V filler, H filler, N filler and Q filler each independently include 50-70 parts of polypropylene, 5-10 parts of activated carbon, 5-10 parts of sodium alginate and 10-20 parts of bacterial suspension embedded in polyvinyl alcohol.

[0020] As a further technical solution, in step a, the step of using polyvinyl alcohol as an embedding agent to embed the bacterial suspension is as follows:

[0021] The bacteria are inoculated into a culture solution and cultured to the late logarithmic phase, and then mixed with polyvinyl alcohol to obtain a polyvinyl alcohol-embedded bacterial suspension.

[0022] As a further technical solution, the culture solution of the bacterial suspension of the V filler includes peptone, beef extract, sodium chloride and water;

[0023] In the V filler, the raw materials of the bacterial suspension embedded in polyvinyl alcohol include: 15-25 parts of polyvinyl alcohol, 10-20 parts of peptone, 2-7 parts of beef extract, 3-5 parts of sodium chloride and 50-70 parts of water.

[0024] As a further technical solution, the culture solution of the bacterial suspension of the H filler includes yeast extract, ammonium chloride, dipotassium hydrogen phosphate, sodium bicarbonate and water;

[0025] In the H filler, the raw materials of the bacterial suspension embedded in polyvinyl alcohol include: 20-30 parts of polyvinyl alcohol, 5-10 parts of yeast extract, 0.5-2 parts of ammonium chloride, 0.5-2 parts of dipotassium hydrogen phosphate and 36-64 parts of water.

[0026] As a further technical solution, the culture solution of the bacterial suspension of the N filler includes peptone, beef extract, sodium chloride and water;

[0027] In the N filler, the raw materials of the bacterial suspension embedded in polyvinyl alcohol include: 15-25 parts of polyvinyl alcohol, 10-20 parts of peptone, 2-7 parts of beef extract, 3-5 parts of sodium chloride and 50-70 parts of water.

[0028] As a further technical solution, the culture solution of the bacterial suspension of the Q filler includes peptone, beef extract, sodium chloride and water;

[0029] Calculated by weight, the raw materials of the bacterial suspension embedded in polyvinyl alcohol in the Q filler include: 20-30 parts of polyvinyl alcohol, 5-15 parts of peptone, 10-30 parts of beef extract, 5-10 parts of sodium chloride and 15-60 parts of water.

[0030] As a further technical solution, the composite filler includes, by weight, 20-40 parts of V filler, 15-35 parts of H filler, 10-30 parts of N filler and 5-25 parts of Q filler.

[0031] As a further technical solution, the bacterial flora loaded on the V filler is Pseudomonas phenolvorans, Pseudomonas stutzeri, yeast, Desulfobacillus phenolsulfatus and Alcaligenes;

[0032] The bacterial flora loaded on the H filler is Rhodopseudomonas palustris, Chlorobacterium sphaeroides, Chromobacterium sulphureum and Thiobacillus thiosulfatum;

[0033] The bacterial flora loaded on the Q filler is Bacillus, Lactobacillus plantarum, Saccharomyces cerevisiae and Nocardia.

[0034] In a second aspect, the present invention provides a composite filler for biological deodorization, which is prepared by the above-mentioned preparation method.

[0035] In a third aspect, the present invention provides application of the above-mentioned composite filler in sewage treatment.

[0036] Compared with the prior art, the present invention has the following beneficial effects:

[0037] The preparation method of the composite filler for biological deodorization provided by the present invention is characterized by simple operation and stable operation. The prepared composite filler has high strength and long service life, is easy for the penetration of nutrients and the proliferation of microorganisms, and can quickly and effectively remove major malodorous gases such as VOCs, H2S, and NH3 in sewage through the coordination between the various fillers. DETAILED DESCRIPTION

[0038] The embodiments of the present invention will be described in detail below in conjunction with the embodiments and examples, but it will be appreciated by those skilled in the art that the following embodiments and examples are only used to illustrate the present invention and should not be considered as limiting the scope of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative work are within the scope of protection of the present invention. If specific conditions are not specified, proceed according to normal conditions or conditions recommended by the manufacturer. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0039] In a first aspect, the present invention provides a method for preparing a composite filler for biological deodorization, wherein the composite filler comprises a V filler, an H filler, an N filler and a Q filler;

[0040] The bacterial flora loaded on the V filler includes at least one of Pseudomonas phenolicus, Pseudomonas stutzeri, yeast, phenol desulfurizing bacteria or alcaligenes, and is mainly used to absorb and remove VOCs;

[0041] The bacterial flora loaded on the H filler includes at least one of Rhodopseudomonas palustris, Chlorobacterium sphaeroides, Chromobacterium sulphureus or Thiobacillus thiosulfurans, and is mainly used for absorbing and removing H2S;

[0042] The bacterial flora loaded on the N filler includes nitrifying bacteria, which are mainly used to absorb and remove NH3;

[0043] The bacterial flora loaded on the Q filler includes at least one of Bacillus, Lactobacillus plantarum, Saccharomyces cerevisiae or Nocardia, which absorbs and removes malodorous gases such as methyl mercaptan, dimethyl disulfide and trimethylamine;

[0044] The preparation method of the V filler, H filler, N filler or Q filler comprises the following steps:

[0045] a. using polyvinyl alcohol as an embedding agent to embed the bacterial suspension to prepare a polyvinyl alcohol-embedded bacterial suspension;

[0046] b. mixing polypropylene, activated carbon and sodium alginate, and then adding a polypropylene foaming agent for foaming to obtain a foamed product;

[0047] c. The foamed product obtained in step b and the polyvinyl alcohol-encapsulated bacterial suspension prepared in step a are mixed, and then pressed and cross-linked in a mold to prepare a V filler, an H filler, an N filler or a Q filler;

[0048] Among the V filler, H filler, N filler and Q filler, the mass fraction of polypropylene can be, for example, but not limited to 50 parts, 60 parts or 70 parts; the mass fraction of activated carbon can be, for example, but not limited to 5 parts, 8 parts or 10 parts; the mass fraction of sodium alginate can be, for example, but not limited to 5 parts, 8 parts or 10 parts; the mass fraction of the bacterial suspension embedded with polyvinyl alcohol can be, for example, but not limited to 10 parts, 15 parts or 20 parts.

[0049] The preparation method of the combined filler for biological deodorization provided by the invention has the characteristics of simple operation and stable operation.

[0050] In some optional embodiments, in step a, the step of embedding the bacterial suspension with polyvinyl alcohol as an embedding agent is as follows:

[0051] The bacteria are inoculated into a culture solution and cultured to the late logarithmic phase, and then mixed with polyvinyl alcohol to obtain a polyvinyl alcohol-embedded bacterial suspension.

[0052] It should be noted that the required types of bacteria loaded in the filler are selected for inoculation.

[0053] In some optional embodiments, if the filler includes multiple bacteria, it is preferred that each bacteria is cultured to the late logarithmic phase and then mixed in equal volumes, and then polyvinyl alcohol is added for embedding.

[0054] In some optional embodiments, the culture solution of the bacterial suspension of the V filler includes peptone, beef extract, sodium chloride and water;

[0055] In the V filler, the raw materials of the bacterial suspension embedded in polyvinyl alcohol include: 15-25 parts of polyvinyl alcohol, 10-20 parts of peptone, 2-7 parts of beef extract, 3-5 parts of sodium chloride and 50-70 parts of water.

[0056] In some optional embodiments, the culture solution of the bacterial suspension of the H filler includes yeast extract, ammonium chloride, dipotassium hydrogen phosphate, sodium bicarbonate and water;

[0057] In the H filler, the raw materials of the bacterial suspension embedded in polyvinyl alcohol include: 20-30 parts of polyvinyl alcohol, 5-10 parts of yeast extract, 0.5-2 parts of ammonium chloride, 0.5-2 parts of dipotassium hydrogen phosphate, 10-20 parts of sodium bicarbonate and 36-64 parts of water.

[0058] In some optional embodiments, in the H filler, the raw materials of the bacterial suspension embedded with polyvinyl alcohol also include 10-20 parts of sodium bicarbonate; the sodium bicarbonate is used as a protective agent and is added to the bacterial suspension simultaneously with polyvinyl alcohol in step a.

[0059] In some optional embodiments, the culture solution of the bacterial suspension of the N filler includes peptone, beef extract, sodium chloride and water;

[0060] In the N filler, the raw materials of the bacterial suspension embedded in polyvinyl alcohol include: 15-25 parts of polyvinyl alcohol, 10-20 parts of peptone, 2-7 parts of beef extract, 3-5 parts of sodium chloride and 50-70 parts of water.

[0061] In some optional embodiments, the culture solution of the bacterial suspension of the Q filler includes peptone, beef extract, sodium chloride and water;

[0062] Calculated by weight, the raw materials of the bacterial suspension embedded in polyvinyl alcohol in the Q filler include: 20-30 parts of polyvinyl alcohol, 5-15 parts of peptone, 10-30 parts of beef extract, 5-10 parts of sodium chloride and 15-60 parts of water.

[0063] The present invention designs culture media suitable for culturing different bacterial species to promote the proliferation of the bacterial species.

[0064] In some optional embodiments, the combined filler includes 20-40 parts of V filler, 15-35 parts of H filler, 10-30 parts of N filler and 5-25 parts of Q filler, by weight.

[0065] Through the coordination of various fillers, the main malodorous gases such as VOCs, H2S, NH3, etc. in sewage can be better removed.

[0066] In some optional embodiments, the bacterial flora loaded on the V filler is Pseudomonas phenolvorans, Pseudomonas stutzeri, yeast, Desulfobacillus phenolsulfatus and Alcaligenes;

[0067] The bacterial flora loaded on the H filler is Rhodopseudomonas palustris, Chlorobacterium sphaeroides, Chromobacterium sulphureum and Thiobacillus thiosulfatum;

[0068] The bacterial flora loaded on the Q filler is Bacillus, Lactobacillus plantarum, Saccharomyces cerevisiae and Nocardia.

[0069] The inventors have found through research that the above-mentioned combination of multiple bacterial flora has a better deodorizing effect than a single bacterial flora.

[0070] In a second aspect, the present invention provides a composite filler for biological deodorization, which is prepared by the above-mentioned preparation method.

[0071] The composite filler prepared by the present invention has high strength, long service life, is easy for nutrients to penetrate and microorganisms to proliferate, and can quickly and effectively remove major malodorous gases such as VOCs, H2S, and NH3 in sewage through the coordination between various fillers.

[0072] In a third aspect, the present invention provides application of the above-mentioned composite filler in sewage treatment.

[0073] The present invention is further described below by means of specific examples and comparative examples. However, it should be understood that these examples are only used for more detailed description and should not be construed as limiting the present invention in any form.

[0074] Comparative Example 1

[0075] A method for preparing a combined filler for biological deodorization (without adding activated carbon):

[0076] V filler preparation:

[0077] Pseudomonas phenolvorans, Pseudomonas stutzeri, and Desulfobacillus phenolvorans were cultured in a formula of 10 parts of peptone, 5 parts of beef extract, 5 parts of sodium chloride, and 60 parts of water until the late logarithmic phase of the bacteria, and then the three bacterial suspensions were mixed in equal amounts, and 60 parts of polyvinyl alcohol was added at the same time, and the mixture was mixed and set aside for use;

[0078] 70 parts of polypropylene (PP) and 10 parts of sodium alginate are mixed evenly, and commercially available PP foaming agent is added. The mixture is placed in a foaming machine and heated to 220±10°C to expand the PP mixture and form a porous structure. The preliminarily foamed PP mixture is placed into 20 parts of immobilized V filler flora, and after soaking and mixing evenly, the mixture is placed in a mold and pressed into shape. During the pressing process, the V filler flora and the PP mixture embedded in polyvinyl alcohol are fully cross-linked and firmly bonded. After the pressing is completed, the V filler is obtained and cut into 2×2mm blocks for standby use.

[0079] H filler preparation:

[0080] Rhodopseudomonas palustris and Thiobacillus thiosulfatus were cultured in a formula of 10 parts of yeast extract, 1 part of ammonium chloride, 1 part of dipotassium hydrogen phosphate, and 53 parts of water until the late logarithmic phase of the strains, and the two bacterial suspensions were mixed in equal amounts, and 40 parts of polyvinyl alcohol and 30 parts of sodium bicarbonate were added at the same time, and the mixture was mixed and set aside for use;

[0081] 70 parts of polypropylene (PP) and 10 parts of sodium alginate are mixed evenly, and commercially available PP foaming agent is added. The mixture is placed in a foaming machine and heated to 220±10°C to expand the PP mixture and form a porous structure. The preliminarily foamed PP mixture is placed into 20 parts of immobilized H filler flora, and after soaking and mixing evenly, the mixture is placed in a mold and pressed into shape. During the pressing process, the H filler flora and PP mixture embedded in polyvinyl alcohol are fully cross-linked and firmly bonded. After the pressing is completed, the H filler is obtained and cut into 3×3mm blocks for standby use.

[0082] N filler preparation:

[0083] Cultivate the nitrifying bacteria in a culture medium of 10 parts of peptone, 5 parts of beef extract, 5 parts of sodium chloride, and 60 parts of water until the late logarithmic phase of the bacteria, and mix in 20 parts of polyvinyl alcohol, which is used as an embedding agent for the V-filler bacterial flora. After mixing, embed and protect the cultured bacterial suspension for later use;

[0084] 70 parts of polypropylene (PP) and 10 parts of sodium alginate are mixed evenly, and commercially available PP foaming agent is added. The mixture is placed in a foaming machine and heated to 220±10°C to expand the PP mixture and form a porous structure. The preliminarily foamed PP mixture is placed into 20 parts of immobilized N filler flora, and after soaking and mixing evenly, the mixture is placed in a mold and pressed into shape. During the pressing process, the N filler flora and PP mixture embedded in polyvinyl alcohol are fully cross-linked and firmly bonded. After the pressing is completed, the N filler is obtained and cut into 3×3mm blocks for standby use.

[0085] Q filler preparation:

[0086] Bacillus subtilis, Lactobacillus plantarum and Nocardia spp. are cultured in a formula of 10 parts of peptone, 15 parts of beef extract, 7 parts of sodium chloride and 43 parts of water to the late logarithmic phase of the strains, and the three bacterial suspensions are mixed in equal amounts, and 75 parts of polyvinyl alcohol are added at the same time, and the mixture is mixed and set aside for use;

[0087] 70 parts of polypropylene (PP) and 10 parts of sodium alginate are mixed evenly, and a commercially available PP foaming agent is added. The mixture is placed in a foaming machine and heated to 220±10°C to expand the PP mixture and form a porous structure. The preliminarily foamed PP mixture is placed into 20 parts of immobilized Q filler flora, and after soaking and mixing evenly, the mixture is placed in a mold and pressed into shape. During the pressing process, the Q filler flora and the PP mixture embedded in polyvinyl alcohol are fully cross-linked and firmly bonded. After the pressing is completed, the Q filler is obtained and cut into 2×2mm blocks for standby use.

[0088] Combination use:

[0089] The filler used in the original deodorization equipment of a sewage treatment plant was ceramsite filter material. After the original system had been in operation for 2 years, it was tested twice a day for 1 week at the designated monitoring point at the sewage treatment plant boundary. The H2S content was 0.56-0.68mg / m 3 、NH3: 3.5-6.7mg / m 3 The odor concentration was 60-72, which could not stably reach the third-level standard of the factory boundary in the "Emission Standard of Odor Pollutants" (GB14554-93). Therefore, 30 parts of V filler without activated carbon, 30 parts of H filler, 20 parts of N filler and 20 parts of Q filler were combined in sequence for the first time, and the ceramsite filter material of the original deodorization system was replaced. After one month of operation, the odor at the monitoring point of the factory boundary was significantly reduced after 10 days of operation. After the odor was significantly reduced after 10 days of operation, the odor detection was carried out at the specified monitoring point twice a day for 20 consecutive days. After testing, H2S was 0.03-0.10mg / m 3 、NH3: 0.9-1.7mg / m 3The odor concentration is 6-15, which can stably reach the secondary standard of the factory boundary in the "Emission Standard of Odor Pollutants" (GB14554-93), indicating that the functional biological filler prepared by the present invention without activated carbon combination has excellent performance and can meet the deodorization function.

[0090] Comparative Example 2

[0091] A method for preparing a combined filler for biological deodorization (single strain filler):

[0092] V filler preparation:

[0093] Pseudomonas phenolvorans is cultured in a formula of 10 parts of peptone, 5 parts of beef extract, 5 parts of sodium chloride, and 60 parts of water until the late logarithmic phase of the strain, and then 20 parts of polyvinyl alcohol is mixed in, and the polyvinyl alcohol is used as an embedding agent for the V-filler bacterial flora. After mixing, the cultured bacterial suspension is embedded and protected for standby use;

[0094] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon and 10 parts of sodium alginate are mixed evenly, and a commercially available PP foaming agent is added. The mixture is placed in a foaming machine and heated to 220±10°C to expand the PP mixture and form a porous structure. The preliminarily foamed PP mixture is placed into 20 parts of immobilized V filler flora, and after soaking and mixing evenly, the mixture is placed in a mold and pressed into shape. During the pressing process, the V filler flora and the PP mixture embedded in polyvinyl alcohol are fully cross-linked and firmly bonded. After the pressing is completed, the V filler is obtained and cut into 2×2mm blocks for standby use.

[0095] H filler preparation:

[0096] The palustris Rhodopseudomonas was cultured in a formula of 10 parts of yeast extract, 1 part of ammonium chloride, 1 part of potassium dihydrogen phosphate, and 53 parts of water until the late logarithmic phase of the strain, and then 20 parts of polyvinyl alcohol and 15 parts of sodium bicarbonate were mixed in, with the polyvinyl alcohol serving as an embedding agent for the V filler flora and the sodium bicarbonate serving as a protective agent for the H filler. After mixing, the cultured bacterial suspension was embedded and protected for standby use;

[0097] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon and 10 parts of sodium alginate are mixed evenly, and a commercially available PP foaming agent is added. The mixture is placed in a foaming machine and heated to 220±10°C to expand the PP mixture and form a porous structure. The preliminarily foamed PP mixture is placed into 20 parts of immobilized H filler flora, and after soaking and mixing evenly, the mixture is placed in a mold and pressed into shape. During the pressing process, the H filler flora and the PP mixture embedded in polyvinyl alcohol are fully cross-linked and firmly bonded. After the pressing is completed, the H filler is obtained and cut into 3×3mm blocks for standby use.

[0098] N filler preparation:

[0099] Cultivate the nitrifying bacteria in a culture medium of 10 parts of peptone, 5 parts of beef extract, 5 parts of sodium chloride, and 60 parts of water until the late logarithmic phase of the bacteria, and mix in 20 parts of polyvinyl alcohol, which is used as an embedding agent for the V-filler bacterial flora. After mixing, embed and protect the cultured bacterial suspension for later use;

[0100] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon and 10 parts of sodium alginate are mixed evenly, and commercially available PP foaming agent is added. The mixture is placed in a foaming machine and heated to 220±10°C to expand the PP mixture and form a porous structure. The preliminarily foamed PP mixture is placed into 20 parts of immobilized N filler flora, and after soaking and mixing evenly, the mixture is placed in a mold and pressed into shape. During the pressing process, the N filler flora and PP mixture embedded in polyvinyl alcohol are fully cross-linked and firmly bonded. After the pressing is completed, the N filler is obtained and cut into 3×3mm blocks for standby use.

[0101] Q filler preparation:

[0102] Bacillus subtilis is cultured in a formula of 10 parts of peptone, 15 parts of beef extract, 7 parts of sodium chloride, and 43 parts of water until the late logarithmic phase of the strain, and then 25 parts of polyvinyl alcohol is mixed in, and the polyvinyl alcohol is used as an embedding agent for the V filler bacterial flora. After mixing, the cultured bacterial suspension is embedded and protected for standby use;

[0103] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon and 10 parts of sodium alginate are mixed evenly, and a commercially available PP foaming agent is added. The mixture is placed in a foaming machine and heated to 220±10°C to expand the PP mixture and form a porous structure. The preliminarily foamed PP mixture is placed into 20 parts of immobilized Q filler flora, and after soaking and mixing evenly, the mixture is placed in a mold and pressed into shape. During the pressing process, the Q filler flora and the PP mixture embedded in polyvinyl alcohol are fully cross-linked and firmly bonded. After the pressing is completed, the Q filler is obtained and cut into 2×2mm blocks for standby use.

[0104] Combination use:

[0105] The filler used in the original deodorization equipment of a sewage treatment plant was ceramsite filter material. After the original system had been in operation for 2 years, it was tested twice a day for 1 week at the designated monitoring point at the sewage treatment plant boundary. The H2S content was 0.56-0.68mg / m 3 、NH3: 3.5-6.7mg / m 3The odor concentration was 60-72, which could not stably reach the third-level standard of the factory boundary in the "Emission Standard of Odor Pollutants" (GB14554-93). Therefore, 30 parts of single strain V filler, 30 parts of H filler, 20 parts of N filler and 20 parts of Q filler were combined in sequence for the second time, and the combined filler without activated carbon replaced for the first time was found to have a relatively rapid performance. After 7 days of operation, the odor at the monitoring point of the factory boundary was significantly reduced. After 7 days of operation, the odor was significantly reduced. After 23 consecutive days of odor detection at the specified monitoring point twice a day, the H2S was tested at 0.10-0.20mg / m 3 、NH3: 1.3-2.6mg / m 3 The odor concentration is 15-36, which can stably reach the third-level standard of the factory boundary in the "Emission Standard of Odor Pollutants" (GB14554-93), indicating that the startup speed of the single-strain combination functional biological filler prepared by the present invention is slightly faster than that of the combined filler with activated carbon, but without the coordination of various deodorizing functional bacteria, the deodorizing effect is slightly lower than that of the combined filler with multiple strains.

[0106] Example 1

[0107] A method for preparing a combined filler for biological deodorization (containing activated carbon multi-strain filler):

[0108] V filler preparation:

[0109] Pseudomonas phenolvorans, Pseudomonas stutzeri, and Desulfobacillus phenolvorans are cultured in a formula of 10 parts of peptone, 5 parts of beef extract, 5 parts of sodium chloride, and 60 parts of water to the late logarithmic phase of the strains, and the three bacterial suspensions are mixed in equal amounts, and 60 parts of polyvinyl alcohol is added at the same time. After mixing, the cultured bacterial suspensions are embedded and protected for standby use;

[0110] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon and 10 parts of sodium alginate are mixed evenly, and a commercially available PP foaming agent is added. The mixture is placed in a foaming machine and heated to 220±10°C to expand the PP mixture and form a porous structure. The preliminarily foamed PP mixture is placed into 20 parts of immobilized V filler flora, and after soaking and mixing evenly, the mixture is placed in a mold and pressed into shape. During the pressing process, the V filler flora and the PP mixture embedded in polyvinyl alcohol are fully cross-linked and firmly bonded. After the pressing is completed, the V filler is obtained and cut into 2×2mm blocks for standby use.

[0111] H filler preparation:

[0112] Rhodopseudomonas palustris and Thiobacillus thiosulfatus were cultured in a formula of 10 parts of yeast extract, 1 part of ammonium chloride, 1 part of dipotassium hydrogen phosphate, and 53 parts of water until the late logarithmic phase of the strains, and the two bacterial suspensions were mixed in equal amounts, and 40 parts of polyvinyl alcohol and 30 parts of sodium bicarbonate were mixed at the same time, and sodium bicarbonate was used as an H filler protective agent. After mixing, the cultured bacterial suspension was embedded and protected for standby use;

[0113] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon and 10 parts of sodium alginate are mixed evenly, and a commercially available PP foaming agent is added. The mixture is placed in a foaming machine and heated to 220±10°C to expand the PP mixture and form a porous structure. The preliminarily foamed PP mixture is placed into 20 parts of immobilized H filler flora, and after soaking and mixing evenly, the mixture is placed in a mold and pressed into shape. During the pressing process, the H filler flora and the PP mixture embedded in polyvinyl alcohol are fully cross-linked and firmly bonded. After the pressing is completed, the H filler is obtained and cut into 3×3mm blocks for standby use.

[0114] N filler preparation:

[0115] Cultivate the nitrifying bacteria in a culture medium containing 10 parts of peptone, 5 parts of beef extract, 5 parts of sodium chloride and 60 parts of water until the late logarithmic phase of the bacteria, add 20 parts of polyvinyl alcohol, mix well, and embed the cultured bacterial suspension for protection for later use;

[0116] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon and 10 parts of sodium alginate are mixed evenly, and commercially available PP foaming agent is added. The mixture is placed in a foaming machine and heated to 220±10°C to expand the PP mixture and form a porous structure. The preliminarily foamed PP mixture is placed into 20 parts of immobilized N filler flora, and after soaking and mixing evenly, the mixture is placed in a mold and pressed into shape. During the pressing process, the N filler flora and PP mixture embedded in polyvinyl alcohol are fully cross-linked and firmly bonded. After the pressing is completed, the N filler is obtained and cut into 3×3mm blocks for standby use.

[0117] Q filler preparation:

[0118] Bacillus, Lactobacillus plantarum and Nocardia are cultured in a formula of 10 parts of peptone, 15 parts of beef extract, 7 parts of sodium chloride and 43 parts of water to the late logarithmic phase of the strains, the three bacterial suspensions are mixed in equal amounts, and 75 parts of polyvinyl alcohol are added at the same time, and after mixing, the cultured bacterial suspensions are embedded and protected for standby use;

[0119] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon and 10 parts of sodium alginate are mixed evenly, and a commercially available PP foaming agent is added. The mixture is placed in a foaming machine and heated to 220±10°C to expand the PP mixture and form a porous structure. The preliminarily foamed PP mixture is placed into 20 parts of immobilized Q filler flora, and after soaking and mixing evenly, the mixture is placed in a mold and pressed into shape. During the pressing process, the Q filler flora and the PP mixture embedded in polyvinyl alcohol are fully cross-linked and firmly bonded. After the pressing is completed, the Q filler is obtained and cut into 2×2mm blocks for standby use.

[0120] Combination use:

[0121] The filler used in the original deodorization equipment of a sewage treatment plant was ceramsite filter material. After the original system had been in operation for 2 years, it was tested twice a day for 1 week at the designated monitoring point at the sewage treatment plant boundary. The H2S content was 0.56-0.68mg / m 3 、NH3: 3.5-6.7mg / m 3 The odor concentration was 60-72, which could not stably reach the third-level standard of the factory boundary in the "Emission Standard of Odor Pollutants" (GB14554-93). Therefore, the third time, the activated carbon multi-bacteria group was combined with 30 parts of V filler, 30 parts of H filler, 20 parts of N filler and 20 parts of Q filler in sequence, and the second single-strain combined filler was replaced. It was found that the performance of the combined filler started quickly. After 4 days of operation, the odor at the factory boundary monitoring point was significantly reduced. After 4 days of operation, the odor was significantly reduced. After 26 days of continuous odor detection at the specified monitoring point twice a day, the H2S was tested at 0.03-0.08mg / m 3 、NH3: 0.8-1.4mg / m 3 The odor concentration is 6-13, which can stably reach the secondary standard of the factory boundary in the "Emission Standard of Odor Pollutants" (GB14554-93), indicating that the combined functional biological filler containing activated carbon and multiple bacterial communities prepared by the present invention has excellent performance, fast startup speed and good deodorizing effect.

[0122] Example 2

[0123] A method for preparing a combined filler for biological deodorization (containing activated carbon multi-strain filler):

[0124] V filler preparation:

[0125] Pseudomonas phenolvorans, Pseudomonas stutzeri and yeast were cultured in a formula of 15 parts of peptone, 2 parts of beef extract, 3 parts of sodium chloride and 50 parts of water until the late logarithmic phase of the strains, the three bacterial suspensions were mixed in equal amounts, and 45 parts of polyvinyl alcohol were added at the same time, and after mixing, the cultured bacterial suspensions were embedded and protected for standby use;

[0126] 50 parts of polypropylene (PP), 5 parts of powdered activated carbon and 5 parts of sodium alginate are mixed evenly, and a commercially available PP foaming agent is added. The mixture is placed in a foaming machine and heated to 220±10°C to expand the PP mixture and form a porous structure. The preliminarily foamed PP mixture is placed into 10 parts of immobilized V filler flora, and after soaking and mixing evenly, the mixture is placed in a mold and pressed into shape. During the pressing process, the V filler flora and the PP mixture embedded in polyvinyl alcohol are fully cross-linked and firmly bonded. After the pressing is completed, the V filler is obtained and cut into 2×2mm blocks for standby use.

[0127] H filler preparation:

[0128] Separately culture the mud green bacteria and the colored bacteria in a formula of 5 parts of yeast extract, 0.5 parts of ammonium chloride, 0.5 parts of dipotassium hydrogen phosphate, and 36 parts of water until the late logarithmic phase of the strains, mix the two bacterial suspensions in equal amounts, and simultaneously add 50 parts of polyvinyl alcohol and 20 parts of sodium bicarbonate, with sodium bicarbonate serving as an H filler protective agent, mix well, and embed and protect the cultured bacterial suspension for later use;

[0129] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon and 10 parts of sodium alginate are mixed evenly, and a commercially available PP foaming agent is added. The mixture is placed in a foaming machine and heated to 220±10°C to expand the PP mixture and form a porous structure. The preliminarily foamed PP mixture is placed into 20 parts of immobilized H filler flora, and after soaking and mixing evenly, the mixture is placed in a mold and pressed into shape. During the pressing process, the H filler flora and the PP mixture embedded in polyvinyl alcohol are fully cross-linked and firmly bonded. After the pressing is completed, the H filler is obtained and cut into 3×3mm blocks for standby use.

[0130] N filler preparation:

[0131] Cultivate the nitrifying bacteria in a culture medium containing 15 parts of peptone, 2 parts of beef extract, 3 parts of sodium chloride and 50 parts of water until the late logarithmic phase of the bacteria, add 15 parts of polyvinyl alcohol, mix well, and embed the cultured bacterial suspension for protection for later use;

[0132] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon and 10 parts of sodium alginate are mixed evenly, and commercially available PP foaming agent is added. The mixture is placed in a foaming machine and heated to 220±10°C to expand the PP mixture and form a porous structure. The preliminarily foamed PP mixture is placed into 20 parts of immobilized N filler flora, and after soaking and mixing evenly, the mixture is placed in a mold and pressed into shape. During the pressing process, the N filler flora and PP mixture embedded in polyvinyl alcohol are fully cross-linked and firmly bonded. After the pressing is completed, the N filler is obtained and cut into 3×3mm blocks for standby use.

[0133] Q filler preparation:

[0134] Bacillus, Lactobacillus plantarum and Saccharomyces cerevisiae are cultured in a formula of 5 parts of peptone, 10 parts of beef extract, 5 parts of sodium chloride and 15 parts of water until the late logarithmic phase of the strains, the three bacterial suspensions are mixed in equal amounts, and 60 parts of polyvinyl alcohol are added at the same time, and after mixing, the cultured bacterial suspensions are embedded and protected for standby use;

[0135] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon and 10 parts of sodium alginate are mixed evenly, and a commercially available PP foaming agent is added. The mixture is placed in a foaming machine and heated to 220±10°C to expand the PP mixture and form a porous structure. The preliminarily foamed PP mixture is placed into 20 parts of immobilized Q filler flora, and after soaking and mixing evenly, the mixture is placed in a mold and pressed into shape. During the pressing process, the Q filler flora and the PP mixture embedded in polyvinyl alcohol are fully cross-linked and firmly bonded. After the pressing is completed, the Q filler is obtained and cut into 2×2mm blocks for standby use.

[0136] Combination use:

[0137] The filler used in the original deodorization equipment of a sewage treatment plant was ceramsite filter material. After the original system had been in operation for 2 years, it was tested twice a day for 1 week at the designated monitoring point at the sewage treatment plant boundary. The H2S content was 0.56-0.68mg / m 3 、NH3: 3.5-6.7mg / m 3 , the odor concentration was 60-72, which could not stably reach the third-level standard of the factory boundary in the "Emission Standard of Malodor Pollutants" (GB14554-93). For the fourth time, 20 parts of V filler, 30 parts of H filler, 30 parts of N filler and 20 parts of Q filler in Example 2 were combined in sequence, and the combined filler in Example 1 was replaced. It was found that the performance of the combined filler started quickly. After 6 days of operation, the odor at the monitoring point of the factory boundary was significantly reduced. After 6 days of operation, the odor was significantly reduced. After 24 days of continuous odor detection at the specified monitoring point twice a day, the H2S was tested at 0.04-0.08mg / m 3 、NH3: 0.9-1.6mg / m 3 The odor concentration is 6-15, which can stably meet the secondary factory boundary standard in the "Emission Standard of Odor Pollutants" (GB14554-93).

[0138] Example 3

[0139] A method for preparing a combined filler for biological deodorization (containing activated carbon multi-strain filler):

[0140] V filler preparation:

[0141] Pseudomonas phenolvorans, Pseudomonas stutzeri, yeast, Desulfobacillus phenolsulfurans, and Alcaligenes are cultured in a formula of 20 parts of peptone, 7 parts of beef extract, 4 parts of sodium chloride, and 70 parts of water to the late logarithmic phase of the strains, and the five bacterial suspensions are mixed in equal amounts, and 125 parts of polyvinyl alcohol is mixed at the same time. The polyvinyl alcohol is used as an embedding agent for the V-filler bacterial flora. After mixing, the cultured bacterial suspension is embedded and protected for standby use;

[0142] 70 parts of polypropylene (PP), 8 parts of powdered activated carbon and 8 parts of sodium alginate are mixed evenly, and a commercially available PP foaming agent is added. The mixture is placed in a foaming machine and heated to 220±10°C to expand the PP mixture and form a porous structure. The preliminarily foamed PP mixture is placed into 15 parts of immobilized V filler flora, and after soaking and mixing evenly, the mixture is placed in a mold and pressed into shape. During the pressing process, the V filler flora and the PP mixture embedded in polyvinyl alcohol are fully cross-linked and firmly bonded. After the pressing is completed, the V filler is obtained and cut into 2×2mm blocks for standby use.

[0143] H filler preparation:

[0144] Rhodopseudomonas palustris, green bacteria, chromogenic bacteria, and Thiobacillus thiosulfatus were cultured in a formula of 8 parts of yeast extract, 2 parts of ammonium chloride, 2 parts of dipotassium hydrogen phosphate, and 64 parts of water until the late logarithmic phase of the strains, and the four bacterial suspensions were mixed in equal amounts, and 120 parts of polyvinyl alcohol and 80 parts of sodium bicarbonate were mixed at the same time, and the sodium bicarbonate was used as an H filler protective agent. After mixing, the cultured bacterial suspension was embedded and protected for standby use;

[0145] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon and 10 parts of sodium alginate are mixed evenly, and a commercially available PP foaming agent is added. The mixture is placed in a foaming machine and heated to 220±10°C to expand the PP mixture and form a porous structure. The preliminarily foamed PP mixture is placed into 20 parts of immobilized H filler flora, and after soaking and mixing evenly, the mixture is placed in a mold and pressed into shape. During the pressing process, the H filler flora and the PP mixture embedded in polyvinyl alcohol are fully cross-linked and firmly bonded. After the pressing is completed, the H filler is obtained and cut into 3×3mm blocks for standby use.

[0146] N filler preparation:

[0147] Cultivate the nitrifying bacteria in a culture medium of 20 parts of peptone, 7 parts of beef extract, 4 parts of sodium chloride and 70 parts of water until the late logarithmic phase of the bacteria, add 25 parts of polyvinyl alcohol, mix well, and embed the cultured bacterial suspension for protection for later use;

[0148] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon and 10 parts of sodium alginate are mixed evenly, and commercially available PP foaming agent is added. The mixture is placed in a foaming machine and heated to 220±10°C to expand the PP mixture and form a porous structure. The preliminarily foamed PP mixture is placed into 20 parts of immobilized N filler flora, and after soaking and mixing evenly, the mixture is placed in a mold and pressed into shape. During the pressing process, the N filler flora and PP mixture embedded in polyvinyl alcohol are fully cross-linked and firmly bonded. After the pressing is completed, the N filler is obtained and cut into 3×3mm blocks for standby use.

[0149] Q filler preparation:

[0150] Bacillus, Lactobacillus plantarum, Saccharomyces cerevisiae and Nocardia spp. are cultured in a formula of 15 parts of peptone, 30 parts of beef extract, 10 parts of sodium chloride and 60 parts of water to the late logarithmic phase of the strains, the three bacterial suspensions are mixed in equal amounts, and 120 parts of polyvinyl alcohol are added at the same time, and after mixing, the cultured bacterial suspensions are embedded and protected for standby use;

[0151] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon and 10 parts of sodium alginate are mixed evenly, and a commercially available PP foaming agent is added. The mixture is placed in a foaming machine and heated to 220±10°C to expand the PP mixture and form a porous structure. The preliminarily foamed PP mixture is placed into 20 parts of immobilized Q filler flora, and after soaking and mixing evenly, the mixture is placed in a mold and pressed into shape. During the pressing process, the Q filler flora and the PP mixture embedded in polyvinyl alcohol are fully cross-linked and firmly bonded. After the pressing is completed, the Q filler is obtained and cut into 2×2mm blocks for standby use.

[0152] Combination use:

[0153] The filler used in the original deodorization equipment of a sewage treatment plant was ceramsite filter material. After the original system had been in operation for 2 years, it was tested twice a day for 1 week at the designated monitoring point at the sewage treatment plant boundary. The H2S content was 0.56-0.68mg / m 3 、NH3: 3.5-6.7mg / m 3 The odor concentration was 60-72, which could not stably reach the third-level standard of the factory boundary in the "Emission Standard of Odor Pollutants" (GB14554-93). For the fifth time, 30 parts of V filler, 30 parts of H filler, 20 parts of N filler and 20 parts of Q filler in Example 3 were combined in sequence, and the combined filler of Example 2 was replaced. It was found that the performance of the combined filler started quickly. After 5 days of operation, the odor at the monitoring point of the factory boundary was significantly reduced. After the odor was significantly reduced after 5 days of operation, the odor detection was carried out at the specified monitoring point twice a day for 25 consecutive days. After testing, H2S was 0.05-0.08mg / m 3 、NH3: 0.8-1.3mg / m 3The odor concentration is 8-17, which can stably meet the secondary factory boundary standard in the "Emission Standard of Odor Pollutants" (GB14554-93).

[0154] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for preparing a composite filler for biological deodorization, characterized in that: The combined fillers include V fillers, H fillers, N fillers and Q fillers; The bacterial flora loaded on the V filler includes at least one of Pseudomonas phenolvorans, Pseudomonas stutzeri, yeast, Desulfobacillus phenolsulfatus or Alcaligenes; The bacterial flora loaded by the H filler includes at least one of Rhodopseudomonas palustris, Chlorobacterium sphaeroides, Chromobacterium sulphureum or Thiobacillus thiosulfurogen; The bacterial flora loaded by the N filler includes nitrifying bacteria; The bacterial flora loaded by the Q filler includes at least one of Bacillus, Lactobacillus plantarum, Saccharomyces cerevisiae or Nocardia; The preparation method of the V filler, H filler, N filler or Q filler comprises the following steps: a. using polyvinyl alcohol as an embedding agent to embed the bacterial suspension to prepare a polyvinyl alcohol-embedded bacterial suspension; b. mixing polypropylene, activated carbon and sodium alginate, and then adding a polypropylene foaming agent for foaming to obtain a foamed product; c. The foamed product obtained in step b and the polyvinyl alcohol-encapsulated bacterial suspension prepared in step a are mixed, and then pressed and cross-linked in a mold to prepare a V filler, an H filler, an N filler or a Q filler; In terms of weight percentage, the V filler, H filler, N filler and Q filler each independently include 50-70 parts of polypropylene, 5-10 parts of activated carbon, 5-10 parts of sodium alginate and 10-20 parts of bacterial suspension embedded in polyvinyl alcohol.

2. The preparation method according to claim 1, characterized in that: In step a, the step of using polyvinyl alcohol as an embedding agent to embed the bacterial suspension is as follows: The bacteria are inoculated into a culture solution and cultured to the late logarithmic phase, and then mixed with polyvinyl alcohol to obtain a polyvinyl alcohol-embedded bacterial suspension.

3. The preparation method according to claim 2, characterized in that: The culture solution of the bacterial suspension of the V filler includes peptone, beef extract, sodium chloride and water; In the V filler, the raw materials of the bacterial suspension embedded in polyvinyl alcohol include: 15-25 parts of polyvinyl alcohol, 10-20 parts of peptone, 2-7 parts of beef extract, 3-5 parts of sodium chloride and 50-70 parts of water.

4. The preparation method according to claim 2, characterized in that: The culture solution of the bacterial suspension of the H filler includes yeast extract, ammonium chloride, dipotassium hydrogen phosphate, sodium bicarbonate and water; In the H filler, the raw materials of the bacterial suspension embedded in polyvinyl alcohol include: 20-30 parts of polyvinyl alcohol, 5-10 parts of yeast extract, 0.5-2 parts of ammonium chloride, 0.5-2 parts of dipotassium hydrogen phosphate and 36-64 parts of water.

5. The preparation method according to claim 2, characterized in that: The culture solution of the bacterial suspension of the N filler includes peptone, beef extract, sodium chloride and water; In the N filler, the raw materials of the bacterial suspension embedded in polyvinyl alcohol include: 15-25 parts of polyvinyl alcohol, 10-20 parts of peptone, 2-7 parts of beef extract, 3-5 parts of sodium chloride and 50-70 parts of water.

6. The preparation method according to claim 2, characterized in that: The culture solution of the bacterial suspension of the Q filler includes peptone, beef extract, sodium chloride and water; Calculated by weight, the raw materials of the bacterial suspension embedded in polyvinyl alcohol in the Q filler include: 20-30 parts of polyvinyl alcohol, 5-15 parts of peptone, 10-30 parts of beef extract, 5-10 parts of sodium chloride and 15-60 parts of water.

7. The preparation method according to claim 1, characterized in that: In terms of mass, the composite filler includes 20-40 parts of V filler, 15-35 parts of H filler, 10-30 parts of N filler and 5-25 parts of Q filler.

8. The preparation method according to claim 1, characterized in that: The bacterial flora loaded on the V filler is Pseudomonas phenolvorans, Pseudomonas stutzeri, yeast, Desulfobacillus phenolsulfurans and Alcaligenes; The bacterial flora loaded on the H filler is Rhodopseudomonas palustris, Chlorobacterium sphaeroides, Chromobacterium sulphureum and Thiobacillus thiosulfatum; The bacterial flora loaded on the Q filler is Bacillus, Lactobacillus plantarum, Saccharomyces cerevisiae and Nocardia.

9. A composite filler for biological deodorization, characterized in that: The preparation method is described in any one of claims 1 to 8.

10. Use of the composite filler according to claim 9 in sewage treatment.