Process for promoting photosynthesis of crops by using acetylisovaleryltylosin tartrate finished product liquid fertilizer

Abstract

The invention belongs to the field of liquid fertilizers, and particularly relates to a method for preparing a liquid fertilizer from acetylisovaleryltylosin tartrate waste liquid to improve the yield of food crops, which comprises the following steps: step 1, fermentation treatment: obtaining a dry acetylisovaleryltylosin tartrate product; step 2, fermenting: fermenting for 36-110 hours, and purifying acetylisovaleryltylosin tartrate from fermentation liquor by using an extraction reagent formed by mixing methanol and ethyl acetate after fermentation is completed; 3, synthesizing: crushing the weighed raw materials by a crusher, and sieving to obtain a material A; the method comprises the following steps: by taking industrial waste corn steep liquor as a main raw material, firstly performing primary precipitation, and then filtering by 50 meshes, 70 meshes and 100 meshes to obtain a material B; putting the material A and the material B into a stirrer, adding water, and stirring and mixing at a temperature higher than room temperature; cooling the mixed raw materials to 20-25 DEG C at room temperature; the liquid fertilizer is prepared from the acetylisovaleryltylosin tartrate waste The liquid fertilizer prepared from the acetylisovaleryltylosin tartrate waste liquid effectively improves the yield of crops.

Description

Technical Field

[0001] The invention belongs to the field of composite liquid fertilizers, in particular to a process for promoting photosynthesis of crops by using tyvalocin finished liquid fertilizer. Background Art

[0002] Liquid compound fertilizer (Fluid Fertilizer), also known as fluid compound fertilizer, commonly known as liquid fertilizer, is a liquid product containing two or more nutrients required by crops. These nutrients are dissolved in water as solutes to form a solution or suspended in water with the help of a suspending agent to form a suspended paste. Liquid fertilizers have been developed to date and there are many varieties, which can be roughly divided into two categories: liquid nitrogen fertilizer and liquid compound fertilizer. The effective substances of liquid nitrogen fertilizer include ammonium, nitrate and amide nitrogen, such as liquid ammonia, ammonia water, and nitrogen solution. Liquid compound fertilizer contains all the nutrients required for plant growth, such as nitrogen, phosphorus, potassium, calcium, magnesium, sulfur and trace elements, etc., and organic substances soluble in water (such as humic acid, amino acids, plant growth regulators, etc.) can also be added. Liquid compound fertilizer can be designed according to the nutritional requirements of crop growth. The fertilizer formula can be adjusted at any time according to the different growth of crops. Scientific formulas can significantly improve fertilizer utilization. The fertilizer efficiency of liquid fertilizers is usually more than 30% higher than that of conventional compound fertilizers. Tylosin, also known as super tylosin, has a chemical name of 3-O-acetyl-4”-O-isovaleryl tylosin. It is an antibiotic specifically for livestock and poultry. Tylosin is a new type of macrolide antibiotic obtained by biotransformation of tylosin by Streptomyces thermotolerans, acetylation at the 3-position and isovalerylation at the 4” position. Due to the change in its chemical structure, it has obvious advantages over tylosin in terms of antibacterial spectrum, antibacterial activity, drug resistance, chemical stability, oral absorbability and blood drug concentration.

[0003] In the prior art, compound liquid fertilizer has the following problems: Insufficient nutrient content: Some liquid fertilizers may have insufficient nutrient content or unreasonable proportions due to production process or raw material problems, which cannot meet the nutrients required for crop growth and thus affect crop yields. Summary of the invention

[0004] In order to make up for the deficiencies of the prior art and solve at least one of the technical problems raised in the background technology, for example, some liquid fertilizers may have insufficient nutrient content or unreasonable proportions due to production process or raw material problems, which cannot meet the nutrients required for crop growth, thereby affecting the crop yield.

[0005] The technical solution adopted by the present invention to solve the technical problem is: a method for preparing liquid fertilizer from tyvalocin waste liquid to increase the yield of grain crops according to the present invention comprises the following steps: Step 1: Fermentation treatment: adding 0.1-1% w / v of adsorbent to the tyvalosin waste liquid for adsorption; the adsorbent is one or more of activated carbon, gluten, gluten hydrolysis residue, diatomaceous earth or chitosan; using a separation medium to separate the adsorbent from the waste liquid; adding acid water to the adsorbent for desorption to obtain a desorption liquid; removing impurities from the desorption liquid by a microfiltration membrane to obtain a purified liquid; spraying the purified liquid to obtain a tyvalosin dry product; Step 2: Fermentation: firstly, fed-batch fermentation is performed, and then converted to repeated fed-batch fermentation, wherein, in the fed-batch fermentation stage, after fermentation for a period of time using a fermentation medium, isobutanol is added to the fermentation broth, and after fermentation for a period of time, glucose is added to the fermentation broth; after fermentation for a period of time, the repeated fed-batch fermentation stage is entered, part of the fermentation broth is discharged, the fermentation medium is added, isobutanol is added to the fermentation broth, and after a period of time, glucose is added to the fermentation broth; the fermentation time is 36-110 hours, and after the fermentation is completed, tyvalosin is purified from the fermentation broth using an extraction reagent mixed with methanol and ethyl acetate; Step 3: Synthesis: The weighed raw materials of each component are crushed by a crusher and then sieved to obtain material A; Industrial waste corn pulp is used as the main raw material, first subjected to a precipitation, and then filtered through 50 mesh, 70 mesh and 100 mesh to obtain material B; Place material A and material B in a blender and add water to mix at a temperature higher than room temperature; The mixed raw materials are cooled to 20-25° C. at room temperature to obtain liquid fertilizer made from tyvalocin waste liquid.

[0006] As a further technical solution of the present invention: the acid water is one of hydrochloric acid or sulfuric acid with a content of 5%-10%, and the amount of acid water is calculated based on 10g / L lactic streptococcus; the pore size of the microfiltration membrane is 0.45um.

[0007] As a further technical solution of the present invention: in the fed-batch fermentation stage, after fermentation for 2 to 3 days using the fermentation medium, 0.10 to 0.30% (v / v) isobutanol is added to the fermentation broth, and after fermentation for 4 to 6 days, glucose is added to the fermentation broth at a flow rate of 3 to 10 g / L / d.

[0008] As a further technical solution of the present invention: after 8 to 12 days of fermentation, the repeated fed-batch fermentation stage is entered, 30 to 50% of the fermentation liquid is discharged, fermentation medium is added and 0.10 to 0.30% (v / v) isobutanol is added to the fermentation liquid, and 2 to 4 days after the start of the repeated fed-batch fermentation stage, glucose is added to the fermentation liquid at a flow rate of 3 to 10 g / L / d, and the repeated fed-batch fermentation stage lasts for 7 to 10 days.

[0009] As a further technical solution of the present invention: the inoculation amount is 0.3% to 10.0% (v / v) of the fermentation medium, the temperature is 25 to 30°C, the pH is 6.8 to 7.6, the DO (dissolved oxygen) is 40% to 60%, the pressure is 0.03 to 0.08 MPa, the ventilation volume is 0.1 to 0.6 VVM, and the stirring speed is 100 to 300 rpm.

[0010] As a further technical solution of the present invention: the inoculation amount is 6.0% (v / v) of the fermentation medium, the temperature is 28°C, the pH is 7.3, the DO is 40%, the pressure is 0.05 MPa, the ventilation volume is 0.4 VVM, and the stirring speed is 160 rpm.

[0011] As a further technical solution of the present invention: weigh the raw materials of each component in parts by weight; 380 million to 450 million parts of tyvalosin, 130 to 140 parts of free amino acids, 1 to 5 parts of calcium, 2 to 6 parts of magnesium, 7 to 11 parts of sulfur, 0.02 to 0.06 parts of boron, 0.01 to 0.05 parts of zinc, 0.08 to 0.12 parts of iron, 0.001 to 0.004 parts of copper, 18 to 26 parts of humic acid, 175 to 215 parts of tea saponin, 120 to 160 parts of fulvic acid, 115 to 130 parts of total nitrogen, 63 to 75 parts of phosphorus pentoxide, and 78 to 86 parts of potassium oxide.

[0012] As a further technical solution of the present invention: the modified tea saponin is prepared by a preparation method comprising the following steps: dissolving tea saponin in an ionic liquid, adding sinapinic acid and a condensing agent, and reacting at a vacuum degree of 0.01-0.05 MPa and 160-190° C. for 2-3 hours under the protection of an inert atmosphere.

[0013] As a further technical solution of the present invention: the condensing agent is a compound of a hydroxyl-containing phenylphosphine oxide derivative and an azodicarboxylate in a molar ratio of 1-1.2:1-1.2, and the amount of the condensing agent is 1-3% of the mass of tea saponin; the ionic liquid is an imidazole ionic liquid and / or a pyridine ionic liquid; and the molar ratio of sinapinic acid to tea saponin is 2-2.2:1.

[0014] The beneficial effects of the present invention are as follows: The invention adds 0.1-1% w / v of adsorbent to tyvalosin waste liquid for adsorption; the adsorbent is one or more of activated carbon, gluten, gluten hydrolysis residue, diatomaceous earth or chitosan; uses a separation medium to separate the adsorbent from the waste liquid; adds acid water to the adsorbent for desorption to obtain a desorption liquid; removes impurities from the desorption liquid by using a microfiltration water membrane to obtain a purified liquid; sprays the purified liquid to obtain a tyvalosin dry product; firstly performs fed-batch fermentation, and then switches to repeated fed-batch fermentation, wherein, in the fed-batch fermentation stage, uses After the fermentation medium is fermented for a period of time, isobutanol is added to the fermentation liquid, and after the fermentation is continued for a period of time, glucose is added to the fermentation liquid; after the fermentation is continued for a period of time, the fermentation is entered into a repeated fed-batch fermentation stage, a part of the fermentation liquid is discharged, the fermentation medium is added, isobutanol is added to the fermentation liquid, and after a period of time, glucose is added to the fermentation liquid; the fermentation time is 36-110 hours, and after the fermentation is completed, tyvalosin is purified from the fermentation liquid with an extraction reagent mixed with methanol and ethyl acetate; the weighed raw materials of each component are crushed by a crusher and then sieved to obtain material A; Industrial waste corn pulp is used as the main raw material, first subjected to a precipitation, and then filtered through 50 mesh, 70 mesh and 100 mesh to obtain material B; material A and material B are placed in a blender and water is added and stirred and mixed at a temperature higher than room temperature; the mixed raw materials are cooled to 20-25° C. at room temperature to obtain liquid fertilizer made from tyvalocin waste liquid; Among them, the liquid fertilizer made from the tyvalocin waste liquid of the present invention effectively increases the yield of crops. DETAILED DESCRIPTION

[0015] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below in conjunction with specific implementation methods.

[0016] Embodiment 1 A method for preparing liquid fertilizer from tyvalocin waste liquid to increase the yield of grain crops according to an embodiment of the present invention comprises the following steps: Step 1: Fermentation treatment: adding 0.1% w / v of an adsorbent to the tyvalosin waste liquid for adsorption; the adsorbent is one or more of activated carbon, gluten, gluten hydrolysis residue, diatomaceous earth or chitosan; using a separation medium to separate the adsorbent from the waste liquid; adding acid water to the adsorbent for desorption to obtain a desorption liquid; removing impurities from the desorption liquid using a microfiltration membrane to obtain a purified liquid; spraying the purified liquid to obtain a tyvalosin dry product; Wherein, the acid water is one of hydrochloric acid or sulfuric acid with a content of 5%, and the amount of acid water is calculated based on 10g / L lactic acid streptococcus; the pore size of the microfiltration membrane is 0.45um; Step 2: Fermentation: firstly, fed-batch fermentation is performed, and then converted to repeated fed-batch fermentation, wherein, in the fed-batch fermentation stage, after fermentation for a period of time using a fermentation medium, isobutanol is added to the fermentation broth, and after fermentation for a period of time, glucose is added to the fermentation broth; after fermentation for a period of time, the repeated fed-batch fermentation stage is entered, part of the fermentation broth is discharged, the fermentation medium is added, isobutanol is added to the fermentation broth, and after a period of time, glucose is added to the fermentation broth; the fermentation time is 36-110 hours, and after the fermentation is completed, tyvalosin is purified from the fermentation broth using an extraction reagent mixed with methanol and ethyl acetate; In the fed-batch fermentation stage, after 2-3 days of fermentation using the fermentation medium, 0.10-0.30% (v / v) isobutanol is added to the fermentation broth, and after 4-6 days of fermentation, glucose is added to the fermentation broth at a flow rate of 3-10 g / L / d; after 8-12 days of fermentation, the repeated fed-batch fermentation stage is entered, 30-50% of the fermentation broth is discharged, the fermentation medium is added, and 0.10% (v / v) isobutanol is added to the fermentation broth, and 2-4 days after the start of the repeated fed-batch fermentation stage, glucose is added to the fermentation broth at a flow rate of 3-10 g / L / d, and the repeated fed-batch fermentation stage lasts for 7-10 days; The parameters are as follows: inoculation amount is 0.3% (v / v) of the fermentation medium, temperature is 25-30°C, pH is 6.8-7.6, DO (dissolved oxygen) is 40%-60%, pressure is 0.03-0.08 MPa, ventilation volume is 0.1-0.6 VVM (air volume / culture volume / min, ventilation ratio), and stirring speed is 100-300 rpm; The inoculum volume was 6.0% (v / v) of the fermentation medium, the temperature was 28°C, the pH was 7.3, the DO was 40%, the pressure was 0.05 MPa, the ventilation volume was 0.4 VVM, and the stirring speed was 160 rpm; Step 3: Synthesis: Weigh the raw materials of each component according to the following weight parts: 380 million parts of tyvalosin, 130 parts of free amino acids, 1 part of calcium, 2 parts of magnesium, 7 parts of sulfur, 0.02 parts of boron, 0.01 parts of zinc, 0.08 parts of iron, 0.001 parts of copper, 18 parts of humic acid, 175 parts of tea saponin, 120 parts of fulvic acid, 115 parts of total nitrogen, 63 parts of phosphorus pentoxide, and 78 parts of potassium oxide; The weighed raw materials of each component are crushed by a crusher and then sieved to obtain material A; Industrial waste corn pulp is used as the main raw material, first subjected to a precipitation, and then filtered through 50 mesh, 70 mesh and 100 mesh to obtain material B; Put material A and material B in a mixer and add water to mix them at a temperature higher than room temperature; cool the mixed raw materials to 20-25° C. at room temperature to obtain liquid fertilizer made from tyvalocin waste liquid; The modified tea saponin is prepared by a preparation method comprising the following steps: dissolving tea saponin in an ionic liquid, adding sinapinic acid and a condensing agent, and reacting for 2-3 hours at a vacuum degree of 0.01-0.05MPa and 160-190°C under inert atmosphere protection; the condensing agent is a compound of a hydroxyl-containing phenylphosphine oxide derivative and an azodicarboxylate in a molar ratio of 1:1, and the amount of the condensing agent is 1% of the mass of the tea saponin; the ionic liquid is an imidazole ionic liquid and / or a pyridine ionic liquid; and the molar ratio of sinapinic acid to tea saponin is 2:1.

[0017] Embodiment 2 A method for preparing liquid fertilizer from tyvalocin waste liquid to increase the yield of grain crops according to an embodiment of the present invention comprises the following steps: Step 1: Fermentation treatment: adding 0.5% w / v of an adsorbent to the tyvalosin waste liquid for adsorption; the adsorbent is one or more of activated carbon, gluten, gluten hydrolysis residue, diatomaceous earth or chitosan; using a separation medium to separate the adsorbent from the waste liquid; adding acid water to the adsorbent for desorption to obtain a desorption liquid; removing impurities from the desorption liquid using a microfiltration membrane to obtain a purified liquid; spraying the purified liquid to obtain a tyvalosin dry product; Wherein, the acid water is one of hydrochloric acid or sulfuric acid with a content of 7%, and the amount of acid water is calculated based on 10g / L lactic acid streptococcus; the pore size of the microfiltration membrane is 0.45um; Step 2: Fermentation: firstly, fed-batch fermentation is performed, and then converted to repeated fed-batch fermentation, wherein, in the fed-batch fermentation stage, after fermentation for a period of time using the fermentation medium, isobutanol is added to the fermentation broth, and after fermentation for a period of time, glucose is added to the fermentation broth; after fermentation for a period of time, the repeated fed-batch fermentation stage is entered, part of the fermentation broth is discharged, the fermentation medium is added, isobutanol is added to the fermentation broth, and after a period of time, glucose is added to the fermentation broth; the fermentation time is 36-110 hours, and after the fermentation is completed, tyvalosin is purified from the fermentation broth using an extraction reagent mixed with methanol and ethyl acetate, In the fed-batch fermentation stage, after 2-3 days of fermentation using the fermentation medium, 0.10-0.30% (v / v) isobutanol is added to the fermentation broth, and after 4-6 days of fermentation, glucose is added to the fermentation broth at a flow rate of 3-10 g / L / d; after 8-12 days of fermentation, the repeated fed-batch fermentation stage is entered, 30-50% of the fermentation broth is discharged, the fermentation medium is added, and 0.10-0.30% (v / v) isobutanol is added to the fermentation broth, and 2-4 days after the start of the repeated fed-batch fermentation stage, glucose is added to the fermentation broth at a flow rate of 3-10 g / L / d, and the repeated fed-batch fermentation stage lasts for 7-10 days; The parameters are as follows: inoculation volume is 5% (v / v) of the fermentation medium, temperature is 25-30°C, pH is 6.8-7.6, DO (dissolved oxygen) is 40%-60%, pressure is 0.03-0.08 MPa, ventilation volume is 0.1-0.6 VVM (air volume / culture volume / min, ventilation ratio), and stirring speed is 100-300 rpm; The inoculum volume was 6.0% (v / v) of the fermentation medium, the temperature was 28°C, the pH was 7.3, the DO was 40%, the pressure was 0.05 MPa, the ventilation volume was 0.4 VVM, and the stirring speed was 160 rpm; Step 3: Synthesis: Weigh the raw materials of each component according to the following weight parts: 400 million parts of tyvalosin, 135 parts of free amino acids, 3 parts of calcium, 4 parts of magnesium, 9 parts of sulfur, 0.04 parts of boron, 0.03 parts of zinc, 0.09 parts of iron, 0.003 parts of copper, 22 parts of humic acid, 195 parts of tea saponin, 140 parts of fulvic acid, 1120 parts of total nitrogen, 70 parts of phosphorus pentoxide, and 82 parts of potassium oxide; The weighed raw materials of each component are crushed by a crusher and then sieved to obtain material A; Industrial waste corn pulp is used as the main raw material, first subjected to a precipitation, and then filtered through 50 mesh, 70 mesh and 100 mesh to obtain material B; Put material A and material B in a mixer and add water to mix them at a temperature higher than room temperature; cool the mixed raw materials to 20-25° C. at room temperature to obtain liquid fertilizer made from tyvalocin waste liquid; The modified tea saponin is prepared by a preparation method comprising the following steps: dissolving tea saponin in an ionic liquid, adding sinapinic acid and a condensing agent, and reacting for 2-3 hours at 160-190°C under the protection of an inert atmosphere and a vacuum degree of 0.01-0.05MPa; the condensing agent is a compound of a hydroxyl-containing phenylphosphine oxide derivative and an azodicarboxylate in a molar ratio of 1.2:1.2, and the amount of the condensing agent is 3% of the mass of the tea saponin; the ionic liquid is an imidazole ionic liquid and / or a pyridine ionic liquid; and the molar ratio of sinapinic acid to tea saponin is 2.2:1.

[0018] Embodiment 3 A method for preparing liquid fertilizer from tyvalocin waste liquid to increase the yield of grain crops according to an embodiment of the present invention comprises the following steps: Step 1: Fermentation treatment: adding 1% w / v of an adsorbent to the tyvalosin waste liquid for adsorption; the adsorbent is one or more of activated carbon, gluten, gluten hydrolysis residue, diatomaceous earth or chitosan; using a separation medium to separate the adsorbent from the waste liquid; adding acid water to the adsorbent for desorption to obtain a desorption liquid; removing impurities from the desorption liquid using a microfiltration membrane to obtain a purified liquid; spraying the purified liquid to obtain a tyvalosin dry product; Wherein, the acid water is one of hydrochloric acid or sulfuric acid with a content of 10%, and the amount of acid water is calculated based on 10g / L lactic acid streptococcus; the pore size of the microfiltration membrane is 0.45um; Step 2: Fermentation: firstly, fed-batch fermentation is performed, and then converted to repeated fed-batch fermentation, wherein, in the fed-batch fermentation stage, after fermentation for a period of time using a fermentation medium, isobutanol is added to the fermentation broth, and after fermentation for a period of time, glucose is added to the fermentation broth; after fermentation for a period of time, the repeated fed-batch fermentation stage is entered, part of the fermentation broth is discharged, the fermentation medium is added, isobutanol is added to the fermentation broth, and after a period of time, glucose is added to the fermentation broth; the fermentation time is 36-110 hours, and after the fermentation is completed, tyvalosin is purified from the fermentation broth using an extraction reagent mixed with methanol and ethyl acetate; In the fed-batch fermentation stage, after 2-3 days of fermentation using the fermentation medium, 0.10-0.30% (v / v) isobutanol is added to the fermentation broth, and after 4-6 days of fermentation, glucose is added to the fermentation broth at a flow rate of 3-10 g / L / d; after 8-12 days of fermentation, the repeated fed-batch fermentation stage is entered, 30-50% of the fermentation broth is discharged, the fermentation medium is added, and 0.10-0.30% (v / v) isobutanol is added to the fermentation broth, and 2-4 days after the start of the repeated fed-batch fermentation stage, glucose is added to the fermentation broth at a flow rate of 3-10 g / L / d, and the repeated fed-batch fermentation stage lasts for 7-10 days; The parameters are as follows: inoculation amount is 10.0% (v / v) of the fermentation medium, temperature is 25-30°C, pH is 6.8-7.6, DO (dissolved oxygen) is 40%-60%, pressure is 0.03-0.08 MPa, ventilation volume is 0.1-0.6 VVM (air volume / culture volume / min, ventilation ratio), and stirring speed is 100-300 rpm; The inoculum volume was 6.0% (v / v) of the fermentation medium, the temperature was 28°C, the pH was 7.3, the DO was 40%, the pressure was 0.05 MPa, the ventilation volume was 0.4 VVM, and the stirring speed was 160 rpm; Step 3: Synthesis: Weigh the raw materials of each component according to the following weight parts: 450 million parts of tyvalosin, 140 parts of free amino acids, 5 parts of calcium, 6 parts of magnesium, 11 parts of sulfur, 0.06 parts of boron, 0.01-0.05 parts of zinc, 0.12 parts of iron, 0.004 parts of copper, 26 parts of humic acid, 215 parts of tea saponin, 160 parts of fulvic acid, 130 parts of total nitrogen, 75 parts of phosphorus pentoxide, and 86 parts of potassium oxide; The weighed raw materials of each component are crushed by a crusher and then sieved to obtain material A; Industrial waste corn pulp is used as the main raw material, first subjected to a precipitation, and then filtered through 50 mesh, 70 mesh and 100 mesh to obtain material B; Put material A and material B in a mixer and add water to mix them at a temperature higher than room temperature; cool the mixed raw materials to 20-25° C. at room temperature to obtain liquid fertilizer made from tyvalocin waste liquid; The modified tea saponin is prepared by a preparation method comprising the following steps: dissolving tea saponin in an ionic liquid, adding sinapinic acid and a condensing agent, and reacting for 2-3 hours at a vacuum degree of 0.01-0.05MPa and 160-190°C under the protection of an inert atmosphere; the condensing agent is a compound of a hydroxyl-containing phenylphosphine oxide derivative and an azodicarboxylate in a molar ratio of 1-1.2:1-1.2, and the amount of the condensing agent is 1-3% of the mass of the tea saponin; the ionic liquid is an imidazole ionic liquid and / or a pyridine ionic liquid; and the molar ratio of sinapinic acid to tea saponin is 2-2.2:1.

[0019] Comparative Example 1 Comparative Example 1 adopts the preparation process of a liquid fertilizer, comprising the following steps: Weigh the following raw materials according to weight: 380 million parts of tyvalosin, 130 parts of free amino acids, 1 part of calcium, 2 parts of magnesium, 7 parts of sulfur, 0.02 parts of boron, 0.01 parts of zinc, 0.08 parts of iron, 0.001 parts of copper, 18 parts of humic acid, 175 parts of tea saponin, 120 parts of fulvic acid, 115 parts of total nitrogen, 63 parts of phosphorus pentoxide, and 78 parts of potassium oxide; The weighed raw materials of each component are crushed by a crusher and then sieved to obtain material A; Industrial waste corn pulp is used as the main raw material, first subjected to a precipitation, and then filtered through 50 mesh, 70 mesh and 100 mesh to obtain material B; Put material A and material B in a mixer and add water to mix them at a temperature higher than room temperature; 5) cool the mixed raw materials to 20-25° C. at room temperature to obtain liquid fertilizer made from tyvalocin waste liquid.

[0020] Performance Testing: For the composite liquid fertilizers prepared in Examples 1-3 and Comparative Example 1, the field test was conducted with wheat cultivation as the experimental object. After the wheat matured, the effect of the liquid fertilizer made from the tyvalosin waste liquid on wheat was obtained based on the wheat yield, and the results are shown in Table 1:

[0021] It can be seen from the above table that the liquid fertilizer prepared from the tyvalocin waste liquid of the present invention effectively increases the yield of crops.

[0022] The above shows and describes the basic principles, main features and advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A method for preparing liquid fertilizer from tyvalocin waste liquid to increase the yield of grain crops, characterized in that: The following steps are involved: Step 1: Fermentation treatment: adding 0.1-1% w / v of adsorbent to the tyvalosin waste liquid for adsorption; the adsorbent is one or more of activated carbon, gluten, gluten hydrolysis residue, diatomaceous earth or chitosan; using a separation medium to separate the adsorbent from the waste liquid; adding acid water to the adsorbent for desorption to obtain a desorption liquid; removing impurities from the desorption liquid by a microfiltration membrane to obtain a purified liquid; spraying the purified liquid to obtain a tyvalosin dry product; Step 2: Fermentation: firstly, a fed-batch fermentation is performed, and then converted to a repeated fed-batch fermentation, wherein, in the fed-batch fermentation stage, after fermentation for a period of time using a fermentation medium, isobutanol is added to the fermentation broth, and after fermentation for a period of time, glucose is added to the fermentation broth; after fermentation for a period of time, the repeated fed-batch fermentation stage is entered, a part of the fermentation broth is discharged, the fermentation medium is added, isobutanol is added to the fermentation broth, and after a period of time, glucose is added to the fermentation broth; the fermentation time is 36-110 hours, and after the fermentation is completed, tyvalosin is purified from the fermentation broth using an extraction reagent mixed with methanol and ethyl acetate; Step 3: Synthesis: The weighed raw materials of each component are crushed by a crusher and then sieved to obtain material A; Industrial waste corn pulp is used as the main raw material, first subjected to a precipitation, and then filtered through 50 mesh, 70 mesh and 100 mesh to obtain material B; Place material A and material B in a blender and add water to mix at a temperature higher than room temperature; The mixed raw materials are cooled to 20-25° C. at room temperature to obtain liquid fertilizer made from tyvalocin waste liquid.

2. The method for preparing liquid fertilizer from tyvalocin waste liquid to increase the yield of grain crops according to claim 1, characterized in that: The acid water is one of hydrochloric acid or sulfuric acid with a content of 5%-10%, and the amount of acid water is calculated based on 10g / L lactic streptococcus; the pore size of the microfiltration membrane is 0.45um.

3. The process for promoting photosynthesis of crops by using a tyvalocin finished liquid fertilizer according to claim 2, characterized in that: In the fed-batch fermentation stage, after 2 to 3 days of fermentation using the fermentation medium, 0.10 to 0.30% (v / v) isobutanol is added to the fermentation broth. After 4 to 6 days of fermentation, glucose is added to the fermentation broth at a flow rate of 3 to 10 g / L / d.

4. The method for preparing liquid fertilizer from tyvalocin waste liquid to increase the yield of grain crops according to claim 3, characterized in that: After 8 to 12 days of fermentation, the repeated fed-batch fermentation stage is entered, 30 to 50% of the fermentation broth is discharged, fermentation medium is added and 0.10 to 0.30% (v / v) isobutanol is added to the fermentation broth. 2 to 4 days after the start of the repeated fed-batch fermentation stage, glucose is added to the fermentation broth at a flow rate of 3 to 10 g / L / d. The repeated fed-batch fermentation stage lasts for 7 to 10 days.

5. The method for preparing liquid fertilizer from tyvalocin waste liquid to increase the yield of grain crops according to claim 4, characterized in that: The inoculation amount is 0.3% to 10.0% (v / v) of the fermentation medium, the temperature is 25 to 30°C, the pH is 6.8 to 7.6, the DO (dissolved oxygen) is 40% to 60%, the pressure is 0.03 to 0.08 MPa, the ventilation volume is 0.1 to 0.6 VVM, and the stirring speed is 100 to 300 rpm.

6. The process for promoting photosynthesis of crops by using tyvalosin finished liquid fertilizer according to claim 5, characterized in that: The inoculation amount was 6.0% (v / v) of the fermentation medium, the temperature was 28°C, the pH was 7.3, the DO was 40%, the pressure was 0.05 MPa, the ventilation volume was 0.4 VVM, and the stirring speed was 160 rpm.

7. The method for preparing liquid fertilizer from tyvalocin waste liquid to increase the yield of grain crops according to claim 6, characterized in that: Weigh the raw materials of each component by weight; 380 million to 450 million parts of tyvalosin, 130 to 140 parts of free amino acids, 1 to 5 parts of calcium, 2 to 6 parts of magnesium, 7 to 11 parts of sulfur, 0.02 to 0.06 parts of boron, 0.01 to 0.05 parts of zinc, 0.08 to 0.12 parts of iron, 0.001 to 0.004 parts of copper, 18 to 26 parts of humic acid, 175 to 215 parts of tea saponin, 120 to 160 parts of fulvic acid, 115 to 130 parts of total nitrogen, 63 to 75 parts of phosphorus pentoxide, and 78 to 86 parts of potassium oxide.

8. The method for preparing liquid fertilizer from tyvalocin waste liquid to increase the yield of grain crops according to claim 8, characterized in that: The modified tea saponin is prepared by a preparation method comprising the following steps: dissolving tea saponin in ionic liquid, adding sinapinic acid and a condensing agent, and reacting at a vacuum degree of 0.01-0.05 MPa and 160-190° C. for 2-3 hours under the protection of an inert atmosphere.

9. The method for preparing liquid fertilizer from tyvalocin waste liquid to increase the yield of grain crops according to claim 9, characterized in that: The condensing agent is a compound of a hydroxyl-containing phenylphosphine oxide derivative and an azodicarboxylate in a molar ratio of 1-1.2:1-1.2, and the amount of the condensing agent is 1-3% of the mass of tea saponin; the ionic liquid is an imidazole ionic liquid and / or a pyridine ionic liquid; and the molar ratio of sinapinic acid to tea saponin is 2-2.2:1.

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