A method for anti-aggregation facilitated dissolution of thermally dried RG-I pectin

Abstract

The application discloses an anti-aggregation and pro-solubility method for hot drying RG-I pectin, and belongs to the technical field of biology. The anti-aggregation and pro-solubility method for hot drying RG-I pectin provided by the application comprises the following steps: (1) mixing peel powder and an acid solution to perform acid extraction, obtaining supernatant after centrifugation; adjusting the pH of the obtained supernatant to 5-7, then performing alcohol precipitation, and obtaining a precipitate through filtration; (2) mixing the precipitate obtained in step (1) and a displacement agent to perform multiple displacements, then performing filtration and hot air drying, and obtaining RG-I pectin. The application fully displaces residual water in the RG-I pectin to increase the free volume of pectin molecules, inhibits the process of adjacent pectin molecular chains from being aggregated due to water molecule evaporation, significantly reduces the aggregation phenomenon between pectin molecules in the drying process, greatly improves the solubility of the pectin, and further improves the application effect of the pectin in various fields.

Description

Technical Field

[0001] This invention belongs to the field of biotechnology, and in particular relates to a method for preventing polymerization and promoting solubility of heat-dried RG-I pectin. Background Technology

[0002] RG-I pectin is an important component of plant cell walls and is abundant in fruit and vegetable processing waste. Due to its many superior functional properties compared to traditional pectin, such as immunomodulation, anti-inflammation, and cancer prevention, RG-I pectin can also be used as a gelling agent, thickener, and heavy metal ion adsorbent, showing great application potential in the food, pharmaceutical, and cosmetic industries.

[0003] Industrially, the preparation of RG-I pectin typically involves extraction, filtration, pH adjustment, alcohol precipitation, and drying. Drying methods include freeze-drying and heat drying. While freeze-dried RG-I pectin exhibits relatively acceptable solubility, its high cost and complex preparation process are generally unacceptable in food processing. Heat drying, on the other hand, is more widely used in pectin drying due to its lower cost and higher production efficiency. However, heat drying often results in poor solubility of RG-I pectin, severely impacting its subsequent processing and use. Therefore, improving the solubility of RG-I pectin is a crucial technical challenge that needs to be addressed in its preparation.

[0004] Currently, existing research mainly modifies the structure of traditional pectin through methods such as substitution, derivatization, and cross-linking to enhance its functional properties, such as solubility, in applications. For example, patent CN116462858A discloses a method of forming conjugates between pectin and amino acids to improve its thermal reversibility and increase its water solubility. However, this method involves structural changes due to the introduction of amino acid groups, and its reliance on two-stage pH control and calcium ions makes the process relatively complex, limiting its production in low-calcium or calcium-free environments. Another approach combines redox systems with grafting modification. For instance, patent CN114716579A discloses grafting pectin with coumaric acid, resulting in a pectin graft copolymer with good solubility and high antioxidant activity. However, this method also introduces phenolic acid groups into the pectin molecule through graft copolymerization, altering the pectin's molecular structure. In addition, there are technologies such as enzymatic viscosity reduction and composite granulation used to produce probiotic-coated pectin, such as patent CN114634655A. This patent produces pectin with low viscosity, good solubility, and superior coating performance. However, enzymatic hydrolysis is a degradation process. While reducing the molecular weight of pectin improves solubility, it also alters the structure and reduces thickening properties such as viscosity. Furthermore, the process requires a large amount of enzyme, resulting in high production costs. The aforementioned processes generally alter the pectin structure, and the compatibilization modification targets traditional pectin. Even using the same preparation process, their solubility will be superior to RG-I pectin.

[0005] Therefore, there is an urgent need for a solubilization method that can not only improve the solubility of RG-I pectin, but also retain the original functions of RG-I pectin to the greatest extent, while achieving no chemical residues, no by-product generation, and meeting economic and environmental protection requirements. Summary of the Invention

[0006] To address the aforementioned technical problems, this invention proposes a method for preventing polymerization and promoting solubility in heat-dried RG-I pectin.

[0007] To achieve the above objectives, the present invention provides the following technical solution:

[0008] This invention provides a method for preventing polymerization and promoting solubility of heat-dried RG-I pectin, comprising the following steps:

[0009] (1) Mix the fruit peel powder and acid solution for acid extraction, centrifuge to obtain the supernatant; adjust the pH of the obtained supernatant to 5-7, then perform alcohol precipitation, filter to obtain the precipitate;

[0010] (2) The precipitate obtained in step (1) is mixed with the displacement agent for displacement, and then filtered and dried with hot air to obtain RG-I pectin.

[0011] Preferably, in step (1), the ratio of fruit peel powder to acid solution is 1:(10-20)(w / v); the acid solution is hydrochloric acid solution; and the pH of the acid solution is 1-3.

[0012] Preferably, in step (1), the acid extraction temperature is 20-60°C and the time is 0.5-2h.

[0013] Preferably, in step (1), the reagent used for alcohol precipitation is anhydrous ethanol; the volume ratio of anhydrous ethanol to supernatant is 2:1; and the alcohol precipitation time is 5 hours.

[0014] Preferably, in step (2), the displacement agent is selected from ethanol and isopropanol.

[0015] Preferably, in step (2), the volume of the displacement agent is 50 to 100 times the mass of the precipitate.

[0016] Preferably, in step (2), the replacement specifically involves: mixing the precipitate obtained in step (1) with the replacement agent and replacing for 5 minutes, filtering, and then adding the replacement agent for the next replacement.

[0017] Preferably, in step (2), when the replacement agent is ethanol, the number of replacements is 3 to 4.

[0018] Preferably, in step (2), when the displacement agent is isopropanol, the number of displacements is 2 to 3.

[0019] Preferably, in step (2), the temperature of the hot air drying is 60°C and the time is 2 hours.

[0020] Compared with the prior art, the present invention has the following advantages and technical effects:

[0021] (1) This invention increases the free volume of pectin molecules by fully replacing the residual water in RG-I pectin, inhibits the affinity aggregation of adjacent pectin molecular chains pulled by the escape of water molecules, significantly reduces the aggregation phenomenon between pectin molecules during the drying process, greatly improves the solubility of pectin, and thus enhances its application effect in various fields.

[0022] (2) The method provided by the present invention does not change the basic structure of RG-I pectin. While improving the solubility of RG-I pectin, it can retain its original functionality, such as gelling and thickening properties, to the greatest extent, thus meeting a wider range of application needs.

[0023] (3) The present invention uses food-grade organic solvents as displacement agents for displacement. They are easily volatilized and leave no residue during the heat drying process, and do not produce harmful by-products, ensuring the safety and environmental friendliness of the product throughout the entire process, which meets the requirements of modern production for green and sustainable production.

[0024] (4) The process of this invention is simple and easy to integrate into existing production lines. It can achieve large-scale production at low cost and high efficiency, and has good industrialization prospects.

[0025] (5) The method of the present invention is not only applicable to the treatment of RG-I pectin, but can also be extended to the treatment process of other similar polysaccharide substances. It has good adaptability and promotion potential, and can meet the needs of different fields for polysaccharide solubility. Attached Figure Description

[0026] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an undue limitation of the invention. In the drawings:

[0027] Figure 1 The diagram shows the microscopic dissolution process of RG-I pectin obtained in Example 1 and Comparative Examples 1-3.

[0028] Figure 2 The diagram shows the microscopic dissolution process of RG-I pectin obtained in Examples 2-3 and Comparative Examples 3-4. Detailed Implementation

[0029] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0030] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0031] This invention provides a method for preventing polymerization and promoting solubility of heat-dried RG-I pectin, comprising the following steps:

[0032] (1) Mix the fruit peel powder and acid solution for acid extraction, centrifuge to obtain the supernatant; adjust the pH of the obtained supernatant to 5-7, then perform alcohol precipitation, filter to obtain the precipitate;

[0033] (2) The precipitate obtained in step (1) is mixed with the displacement agent for displacement, and then filtered and dried with hot air to obtain RG-I pectin.

[0034] The reason for the poor solubility of heat-dried RG-I pectin remains unclear. The inventors speculate that it is related to the aggregation of hydrophilic pectin chains induced by water molecules during the heat drying process. Therefore, the inventors attempted to increase the free volume of pectin molecules by replacing the residual water in RG-I pectin, thereby inhibiting the affinity aggregation of adjacent pectin molecular chains pulled by the escape of water molecules, and achieving the effect of improving resolubility after drying.

[0035] In a preferred embodiment, in step (1), the fruit peel powder includes citrus peel powder.

[0036] In a preferred embodiment, step (1) includes the following steps: drying the fruit peel at 50-60°C and pulverizing it to obtain the fruit peel powder.

[0037] In a preferred embodiment, in step (1), the ratio of fruit peel powder to acid solution is 1:(10-20)(w / v), that is, 10-20 mL of acid solution is used for every 1g of fruit peel powder; more preferably, it is 1:(15-20)(w / v); and in an even more preferred embodiment, the ratio of fruit peel powder to acid solution is 1:20 (g / mL).

[0038] In a preferred embodiment, in step (1), the acid solution is a hydrochloric acid solution; the pH of the acid solution is 1 to 3, more preferably 1 to 2.

[0039] In a preferred embodiment, in step (1), the temperature of acid extraction is 20-60°C, more preferably 20-40°C; and the time of acid extraction is 0.5-2h, more preferably 0.5-1h.

[0040] In a preferred embodiment, in step (1), the pH of the obtained supernatant is adjusted to 5-7, more preferably 5.

[0041] In a preferred embodiment, in step (1), the reagent used for alcohol precipitation is anhydrous ethanol; the volume ratio of anhydrous ethanol to supernatant is 2:1; and the alcohol precipitation time is 5 hours.

[0042] In a preferred embodiment, in step (2), the displacement agent is selected from ethanol and isopropanol. This invention uses food-grade organic solvents as displacement agents, which are easily volatile and leave no residue during the heat drying process, and do not produce harmful byproducts, ensuring the safety and environmental friendliness of the product throughout the entire process.

[0043] In a preferred embodiment, in step (2), the volume of the displacement agent is 50 to 100 times the mass of the precipitate. The amount of displacement agent used in this invention affects the solubility of RG-I pectin. Insufficient displacement agent cannot adequately displace the water in RG-I pectin, while excessive displacement agent wastes reagents and does not significantly improve pectin solubility.

[0044] In a preferred embodiment, in step (2), the replacement specifically involves: mixing the precipitate and the replacement agent, replacing for 5 minutes, filtering, and then adding the replacement agent for the next replacement.

[0045] In a preferred embodiment, in step (2), when the displacing agent is ethanol, the number of displacing operations is 3 to 4 times; when the displacing agent is isopropanol, the number of displacing operations is 2 to 3 times. Too few displacing operations will not sufficiently displace the water in the RG-I pectin, affecting the solubility of the RG-I pectin.

[0046] In a preferred embodiment, in step (2), the temperature of the hot air drying is 60°C and the time is 2 hours.

[0047] Unless otherwise specified, all raw materials used in the embodiments of this invention were purchased through commercial channels.

[0048] Example 1

[0049] A method for preventing polymerization and promoting solubility of heat-dried RG-I pectin, the specific steps of which are as follows:

[0050] (1) The citrus peel was dried at 50℃ and pulverized to obtain citrus peel powder; the above citrus peel powder was mixed with hydrochloric acid solution of pH=1 at a material-to-liquid ratio of 1:20 (g / mL), extracted at 40℃ for 1h, centrifuged, the supernatant was collected and the pH of the supernatant was adjusted to 5, and then anhydrous ethanol was added to the above supernatant at a volume ratio of 2:1, allowed to stand for 5h, filtered through a filter cloth to obtain the precipitate;

[0051] (2) Mix the precipitate obtained in step (1) with ethanol for 5 min, filter, and then add ethanol for the next replacement. Perform a total of 3 replacements. The volume of ethanol used for each replacement is 100 times the mass of the precipitate. The residual water is continuously replaced by ethanol. After the replacement is completed, filter and then dry with hot air at 60°C. After 2 h, RG-I pectin is obtained.

[0052] The solubility of RG-I pectin obtained in Example 1 was measured to be 79.67%, and the bulk density of RG-I pectin obtained in Example 1 was measured to be 0.658 g / cm³ using the powder assay method. 3 .

[0053] Comparative Example 1

[0054] A method for preventing polymerization and promoting solubility of heat-dried RG-I pectin, the specific steps of which are as follows:

[0055] (1) The citrus peel was dried at 50℃ and pulverized to obtain citrus peel powder; the above citrus peel powder was mixed with hydrochloric acid solution of pH=1 at a material-to-liquid ratio of 1:20 (g / mL), extracted at 40℃ for 1h, centrifuged, the supernatant was collected and the pH of the supernatant was adjusted to 5, and then anhydrous ethanol was added to the above supernatant at a volume ratio of 2:1, allowed to stand for 5h, filtered through a filter cloth to obtain the precipitate;

[0056] (2) Mix the precipitate obtained in step (1) with ethanol for 5 min, filter, and then add ethanol for the next replacement. Perform a total of 2 replacements. The volume of ethanol used for each replacement is 100 times the mass of the precipitate. The residual water is continuously replaced by ethanol. After the replacement is completed, filter and then dry with hot air at 60°C. After 2 h, RG-I pectin is obtained.

[0057] The solubility of RG-I pectin obtained in Comparative Example 1 was measured to be 68.92%, and the bulk density of RG-I pectin obtained in Comparative Example 1, determined by the powder assay method, was 0.708 g / cm³. 3 .

[0058] Comparative Example 2

[0059] A method for preparing heat-dried RG-I pectin, the specific steps of which are as follows:

[0060] (1) The citrus peel was dried at 50℃ and pulverized to obtain citrus peel powder; the above citrus peel powder was mixed with hydrochloric acid solution of pH=1 at a material-to-liquid ratio of 1:20 (g / mL), extracted at 40℃ for 1h, centrifuged, the supernatant was collected and the pH of the supernatant was adjusted to 5, and then anhydrous ethanol was added to the above supernatant at a volume ratio of 2:1, allowed to stand for 5h, filtered through a filter cloth to obtain the precipitate;

[0061] (2) The precipitate obtained in step (1) was mixed with ethanol and replaced for 5 min. The volume of ethanol was 100 times the mass of the precipitate. The residual water was continuously replaced by ethanol. After the replacement was completed, the mixture was filtered and then dried with hot air at 60°C for 2 h to obtain RG-I pectin.

[0062] The solubility of RG-I pectin obtained in Comparative Example 2 was measured to be 49.06%, and the bulk density of RG-I pectin obtained in Comparative Example 2, determined by the powder assay method, was 0.714 g / cm³. 3 .

[0063] Comparative Example 3

[0064] A method for preparing heat-dried RG-I pectin, the specific steps of which are as follows:

[0065] (1) The citrus peel was dried at 50℃ and pulverized to obtain citrus peel powder; the above citrus peel powder was mixed with hydrochloric acid solution of pH=1 at a material-to-liquid ratio of 1:20 (g / mL), extracted at 40℃ for 1h, centrifuged, the supernatant was collected and the pH of the supernatant was adjusted to 5, and then anhydrous ethanol was added to the above supernatant at a volume ratio of 2:1, allowed to stand for 5h, filtered through a filter cloth to obtain the precipitate;

[0066] (2) The precipitate obtained in step (1) was dried by hot air at 60°C for 2 hours to obtain RG-I pectin.

[0067] The solubility of RG-I pectin obtained in Comparative Example 3 was measured to be 23.91%, and the bulk density of RG-I pectin obtained in Comparative Example 3, determined by the powder assay method, was 0.767 g / cm³. 3 .

[0068] Test Example 1: A small amount of RG-I pectin powder obtained in Example 1 and Comparative Examples 1-3 was placed on a glass slide, and a drop of distilled water was added. The dissolution process of the pectin was observed under an inverted microscope. The results are shown in [Figure 1]. Figure 1 .

[0069] Figure 1 This diagram illustrates the microscopic dissolution process of RG-I pectin obtained in Examples 1 and Comparative Examples 1-3. In the diagram, white areas represent aqueous solutions, and black areas represent pectin powder. Over time, as the pectin continues to diffuse and dissolve in the water, the black areas of the pectin decrease. Figure 1It can be seen that the RG-I pectin prepared in Example 1 dissolved the fastest, indicating that it has a high solubility. In contrast, the RG-I pectin prepared in Comparative Example 2 dissolved more slowly; even after 480 seconds, most of the pectin remained undissolved, indicating that a significant amount of insoluble portion still exists. Therefore, the RG-I pectin prepared using the anti-polymerization and solubilization method provided by this invention exhibits better solubility.

[0070] The residual water content was indirectly tested by HPLC. The final residual water content of RG-I pectin prepared in Example 1 was 0.45%, the residual water content of RG-I pectin prepared in Comparative Examples 1 and 2 was 3.30% and 3.68%, respectively, and the residual water content of RG-I pectin prepared in Comparative Example 3 was 31.97%. It can be seen that the more residual water is replaced, the better the solubility of RG-I pectin.

[0071] Example 2

[0072] A method for preventing polymerization and promoting solubility of heat-dried RG-I pectin, the specific steps of which are as follows:

[0073] (1) The citrus peel was dried at 50℃ and pulverized to obtain citrus peel powder; the above citrus peel powder was mixed with hydrochloric acid solution of pH=1 at a material-to-liquid ratio of 1:20 (g / mL), extracted at 40℃ for 1h, centrifuged, the supernatant was collected and the pH of the supernatant was adjusted to 5, and then anhydrous ethanol was added to the above supernatant at a volume ratio of 2:1, allowed to stand for 5h, filtered through a filter cloth to obtain the precipitate;

[0074] (2) Mix the precipitate obtained in step (1) with isopropanol and replace for 5 minutes. After filtration, add isopropanol for the next replacement. Repeat the replacement for a total of 3 times. The volume of isopropanol used for each replacement is 100 times the mass of the precipitate. The residual water is continuously replaced by isopropanol. After the replacement is completed, filter and then dry with hot air at 60°C. After 2 hours, RG-I pectin is obtained.

[0075] The solubility of RG-I pectin obtained in Example 2 was measured to be 77.85%, and the bulk density of RG-I pectin obtained in Example 2 was measured to be 0.543 g / cm³ using the powder assay method. 3 .

[0076] Example 3

[0077] A method for preparing heat-dried RG-I pectin, the specific steps of which are as follows:

[0078] (1) The citrus peel was dried at 50℃ and pulverized to obtain citrus peel powder; the above citrus peel powder was mixed with hydrochloric acid solution of pH=1 at a material-to-liquid ratio of 1:20 (g / mL), extracted at 40℃ for 1h, centrifuged, the supernatant was collected and the pH of the supernatant was adjusted to 5, and then anhydrous ethanol was added to the above supernatant at a volume ratio of 2:1, allowed to stand for 5h, filtered through a filter cloth to obtain the precipitate;

[0079] (2) Mix the precipitate obtained in step (1) with isopropanol and replace for 5 minutes. After filtration, add isopropanol for the next replacement. Repeat the replacement twice. The volume of isopropanol used for each replacement is 100 times the mass of the precipitate. The residual water is continuously replaced by isopropanol. After the replacement is completed, filter and then dry with hot air at 60°C. After 2 hours, RG-I pectin is obtained.

[0080] The solubility of RG-I pectin obtained in Example 3 was measured to be 75.40%, and the bulk density of RG-I pectin obtained in Example 3 was measured to be 0.615 g / cm³ using the powder assay method. 3 .

[0081] Comparative Example 4

[0082] A method for preparing heat-dried RG-I pectin, the specific steps of which are as follows:

[0083] (1) The citrus peel was dried at 50℃ and pulverized to obtain citrus peel powder; the above citrus peel powder was mixed with hydrochloric acid solution of pH=1 at a material-to-liquid ratio of 1:20 (g / mL), extracted at 40℃ for 1h, centrifuged, the supernatant was collected and the pH of the supernatant was adjusted to 5, and then anhydrous ethanol was added to the above supernatant at a volume ratio of 2:1, allowed to stand for 5h, filtered through a filter cloth to obtain the precipitate;

[0084] (2) The precipitate obtained in step (1) is mixed with isopropanol and replaced for 5 min. The volume of isopropanol is 100 times the mass of the precipitate. The residual water is continuously replaced by isopropanol. After the replacement is completed, the mixture is filtered and then dried with hot air at 60°C. After 2 h, RG-I pectin is obtained.

[0085] The solubility of RG-I pectin obtained in Comparative Example 4 was measured to be 64.47%, and the bulk density of RG-I pectin obtained in Comparative Example 4, determined by the powder assay method, was 0.642 g / cm³. 3 .

[0086] Test Example 2: A small amount of RG-I pectin powder obtained in Examples 2-3 and Comparative Examples 3-4 was placed on a glass slide, and a drop of distilled water was added. The dissolution process of the pectin was observed under an inverted microscope. The results are shown in [Figure 1]. Figure 2 .

[0087] Figure 2This diagram illustrates the microscopic dissolution process of RG-I pectin obtained in Examples 2-3 and Comparative Examples 3-4. In the diagram, white areas represent aqueous solutions, and black areas represent pectin powder. Over time, as the pectin continues to diffuse and dissolve in the water, the black areas of the pectin decrease. Figure 2 It can be seen that the RG-I pectin prepared in Example 2 dissolved the fastest, indicating that it has a high solubility. In contrast, the RG-I pectin prepared in Comparative Example 3 dissolved more slowly; even after 480 seconds, most of the pectin remained undissolved, indicating that a significant amount of black areas still existed. Therefore, the RG-I pectin prepared using the anti-polymerization and solubilization method provided by this invention has better solubility.

[0088] The residual water content was indirectly tested by HPLC. The final residual water contents of RG-I pectin prepared in Example 2 and Example 3 were 0.44% and 1.50%, respectively. The residual water content of RG-I pectin prepared in Comparative Example 4 was 6.23%, and the residual water content of RG-I pectin prepared in Comparative Example 3 was 31.97%. It can be seen that the more residual water is replaced, the better the solubility of RG-I pectin.

[0089] Comparative Example 5

[0090] A method for preparing heat-dried RG-I pectin, the specific steps of which are as follows:

[0091] (1) The citrus peel was dried at 50℃ and pulverized to obtain citrus peel powder; the above citrus peel powder was mixed with hydrochloric acid solution of pH=1 at a material-to-liquid ratio of 1:20 (g / mL), extracted at 40℃ for 1h, centrifuged, the supernatant was collected and the pH of the supernatant was adjusted to 5, and then anhydrous ethanol was added to the above supernatant at a volume ratio of 2:1, allowed to stand for 5h, filtered through a filter cloth to obtain the precipitate;

[0092] (2) Mix the precipitate obtained in step (1) with acetone for 5 min, filter, and then add acetone for the next replacement. Perform a total of 3 replacements. The volume of acetone used for each replacement is 100 times the mass of the precipitate. By continuously replacing the residual water with acetone, filter, and dry with hot air at 60°C for 2 h, RG-I pectin is obtained.

[0093] The soluble rate of RG-I pectin obtained in Comparative Example 5 was measured to be 57.74%.

[0094] The above are merely preferred embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in the present invention should be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. An anti-aggregation facilitated solubilization method of thermally dried RG-I pectin, characterized in that, The method comprises the following steps: (1) mixing peel powder and acid solution for acid extraction, and obtaining supernatant after centrifugation; adjusting the pH of the supernatant to 5-7, and then performing alcohol precipitation, and obtaining precipitate after filtration; the temperature of the acid extraction is 20-40℃, and the time is 0.5-2h; (2) mixing the precipitate obtained in step (1) and a displacement agent for displacement, and then performing filtration and hot air drying to obtain RG-I pectin; the displacement agent is selected from one of ethanol and isopropanol; the volume of the displacement agent is 50-100 times the mass of the precipitate; the displacement is specifically as follows: mixing the precipitate obtained in step (1) and the displacement agent, and then displacing for 5min, and then adding the displacement agent for the next displacement after filtration; when the displacement agent is ethanol, the number of displacements is 3-4 times; when the displacement agent is isopropanol, the number of displacements is 2-3 times.

2. The anti-aggregation facilitated solubilization process of heat-dried RG-I pectin according to claim 1, characterized in that, In step (1), the ratio of the peel powder to the acid solution is 1:(10-20)(w / v); the acid solution is a hydrochloric acid solution; and the pH of the acid solution is 1-3.

3. The anti-aggregation facilitated solubilization process of heat-dried RG-I pectin according to claim 1, characterized in that, In step (1), the reagent used for the alcohol precipitation is anhydrous ethanol, the volume ratio of the anhydrous ethanol to the supernatant is 2:1, and the time of the alcohol precipitation is 5h.

4. The anti-aggregation facilitated solubilization process of heat-dried RG-I pectin according to claim 1, characterized in that, In step (2), the temperature of the hot air drying is 60℃, and the time is 2h.

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