Full-automatic nucleic acid extraction equipment

Abstract

The invention relates to the technical field of medical equipment, and provides full-automatic nucleic acid extraction equipment which comprises a machine body, a cabinet door, a base and a guide mechanism, and an extraction cavity is formed in the peripheral side of the machine body; a first sliding groove and at least one second sliding groove are formed in the side wall in the extraction cavity; the guide mechanism comprises a sliding seat, a connecting rod and a wheel disc; the sliding seat is arranged in the extraction cavity in a sliding manner; the two ends of the connecting rod are rotationally arranged on the sliding seat and the base respectively; the wheel disc is fixedly or rotationally arranged on the connecting rod and is in sliding or rolling connection with the first sliding groove and the second sliding groove. According to the test tube rack, the sliding seats and the connecting rods are arranged, the connecting rods are arranged in parallel and at intervals, the base can stably slide out of or slide into the extraction cavity so as to facilitate installation of the test tube rack, and the base sliding out of the extraction cavity can be fixed at different heights by arranging the first sliding groove and the second sliding groove; therefore, the nucleic acid extraction equipment is suitable for different use conditions.

Description

Technical Field

[0001] The present invention relates to the technical field of medical equipment, and in particular to a fully automatic nucleic acid extraction device. Background Art

[0002] Nucleic acid extraction equipment is a type of instrument specially designed to separate and purify nucleic acids (DNA or RNA) from biological samples (such as blood, tissues, cells, viruses, etc.). It usually integrates advanced biotechnology and automation technology to complete the nucleic acid extraction process in an efficient, accurate and reproducible manner.

[0003] The invention with announcement number CN115386457B discloses a nucleic acid extractor, which includes a reagent strip assembly, a pipetting module, a magnetic separation assembly and a heating assembly; the reagent strip assembly includes a reagent strip base and a reagent strip, and the reagent strip base is provided with a plurality of reagent strips along its length direction, and the reagent strip includes a heating tube and a magnetic separation tube; the pipetting module is arranged above the reagent strip base, and the pipetting module includes a pipetting pump group, a first driving component and a second driving component, the first driving component is used to drive the pipetting pump group to move up and down, and the second driving component is used to drive the pipetting pump group to move back and forth along the length direction of the reagent strip; the magnetic separation assembly includes a magnet group and a fourth driving component, the magnet group is arranged below the magnetic separation tube, and the fourth driving component is used to drive the magnet group to move up and down; the heating assembly is arranged below the heating tube.

[0004] In the above technical solution, in order to provide a safe and stable operating environment for nucleic acid extraction, the bottom plate for fixing the test tube rack is arranged inside the shell. However, the operating space inside the shell is relatively small, which makes it inconvenient to connect and fix the test tube rack with the bottom plate, which not only reduces the installation efficiency of the test tube rack, but also is not conducive to ensuring the firmness of the fixation of the test tube rack. Summary of the invention

[0005] In view of this, the present invention proposes a fully automatic nucleic acid extraction device, which can quickly and firmly install a test tube rack on a base, thereby improving the operating efficiency of the nucleic acid extraction device.

[0006] The technical solution of the present invention is implemented as follows: The present invention provides a fully automatic nucleic acid extraction device, including a body, a cabinet door, a base and a guide mechanism, wherein:

[0007] An extraction cavity is provided on the peripheral side of the body;

[0008] The cabinet door is detachably fixed to the machine body and blocks the extraction chamber. A first slide groove and at least one second slide groove are provided on the side wall of the extraction chamber. An end of the second slide groove away from the cabinet door is connected to the first slide groove, and an end of the second slide groove close to the cabinet door is located above an end of the first slide groove close to the cabinet door.

[0009] The guiding mechanism includes a sliding seat, a connecting rod and a wheel disc. The sliding seat is slidably arranged in the extraction chamber; the two ends of the connecting rod are rotatably arranged on the sliding seat and the base respectively, and a group of connecting rods are respectively arranged at both ends of the base, and each group of connecting rods is provided with a plurality of connecting rods, and the plurality of connecting rods are arranged in parallel and at intervals; the wheel disc is fixedly or rotatably arranged on the connecting rod, and is slidably or rollingly connected to the first slide groove and the second slide groove.

[0010] On the basis of the above technical solution, preferably, the first chute is arranged along the horizontal direction;

[0011] The second slide groove includes a horizontal section and a connecting section, and the multiple horizontal sections are parallel to the first slide groove and are arranged at intervals; the two ends of the connecting section are respectively connected to the end of the horizontal section away from the cabinet door and the first slide groove, the connecting section is arc-shaped, and the multiple connecting sections have the same center but different radii and arc lengths.

[0012] More preferably, the sliding resistance of the slide seat in the body is greater than the moving resistance of the wheel disc in the second slide groove and the rotation resistance of the connecting rod;

[0013] The second slide grooves are provided with two, the connecting section away from the cabinet door is connected with the end of the first slide groove away from the cabinet door, and when the wheel disc moves to the horizontal section located above, the end of the connecting rod close to the wheel disc is located above the end of the connecting rod away from the wheel disc, and when the wheel disc moves to the horizontal section located below or into the first slide groove, the end of the connecting rod close to the wheel disc is located below the end of the connecting rod away from the wheel disc.

[0014] More preferably, the wheel disc comprises a fixed rod, a movable disc and two rotating discs, wherein:

[0015] The two ends of the fixed rod are rotatably arranged on the two connecting rods of the same group respectively;

[0016] The movable plate is fixedly arranged on the fixed rod, and is slidably arranged in the first sliding groove and the second sliding groove;

[0017] The two rotating disks are both rotatably arranged on the movable disk and are respectively rollingly connected to the first slide groove and the upper and lower sides of the horizontal section. The two rotating disks are rollingly connected, and the outer diameter of the movable disk is less than 2 times the outer diameter of the rotating disk.

[0018] More preferably, the wheel disc further comprises a sliding rod and a spring, wherein:

[0019] The sliding rod is slidably arranged on the moving plate;

[0020] The spring is fixedly arranged on the moving plate, and one end of the spring abuts against one end of the sliding rod, so that the other end of the sliding rod abuts against a side of the connecting section close to the cabinet door.

[0021] More preferably, each movable plate is provided with two sliding rods and two springs, the sliding rods and the springs correspond one to one, and the two sliding rods are respectively abutted against a side of the connecting section close to the cabinet door and a side of the connecting section away from the cabinet door.

[0022] On the basis of the above technical solution, preferably, a mounting cavity is provided on a side of the machine body away from the extraction cavity, and the mounting cavity is communicated with the extraction cavity and is used for mounting a driving component of a pipetting module.

[0023] More preferably, it also includes a limiter, and the base includes a support seat, a sliding frame and connecting bolts, wherein,

[0024] The support seat is rotatably connected to the connecting rod;

[0025] The sliding frame is slidably disposed on the bottom side of the support seat, and its sliding direction is parallel to the opening direction of the first sliding groove;

[0026] The connecting bolt is connected to the supporting seat through threaded engagement and can be clamped with the sliding frame;

[0027] The limiter is rotatably arranged on the machine body and is in contact with and rolling connection with an end of the sliding frame close to the connecting rod. The cross section of the limiter is non-circular.

[0028] More preferably, two limiters are provided, the two limiters are respectively abutted against and rollingly connected to the two ends of the sliding frame, and the cross-section of the limiter is a Lello triangle.

[0029] On the basis of the above technical solution, preferably, the distance between one end of the multiple horizontal sections close to the cabinet door and the cabinet door gradually decreases from top to bottom, and the distance between one end of the horizontal section close to the cabinet door and the cabinet door is greater than the distance between one end of the first sliding groove close to the cabinet door and the cabinet door.

[0030] The fully automatic nucleic acid extraction device of the present invention has the following beneficial effects compared with the prior art:

[0031] (1) By providing a slide seat and a connecting rod, and arranging a plurality of connecting rods in parallel and at intervals, the base can be smoothly slid out of or into the extraction chamber to facilitate the installation of the test tube rack. By providing a first slide groove and a second slide groove, the base that slides out of the extraction chamber can be fixed at different heights, so that the nucleic acid extraction device can be adapted to different operating conditions;

[0032] (2) By providing a fixed rod, a movable plate and a wheel plate, the wheel plate is allowed to roll against the top and bottom sides of the first slide groove and the horizontal section, and by providing a sliding rod and a spring, the sliding rod is allowed to slide against the side wall of the connecting section, so that the wheel plate can move stably, thereby ensuring the stability of movement of the base;

[0033] (3) By configuring the base to include a support seat, a sliding frame and connecting bolts, and providing a limiter with a non-circular cross-section on the body, the limiter is used to abut against the sliding frame, which can not only limit the movement of the base, but also reduce the space occupied by the nucleic acid extraction device along the direction in which the extraction chamber is opened. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0035] Figure 1 A three-dimensional diagram of a fully automatic nucleic acid extraction device of the present invention;

[0036] Figure 2 It is a cross-sectional view of the second chute in a fully automatic nucleic acid extraction device of the present invention;

[0037] Figure 3 It is a three-dimensional diagram of a guide mechanism in a fully automatic nucleic acid extraction device of the present invention;

[0038] Figure 4 A cross-sectional view of a fully automatic nucleic acid extraction device of the present invention;

[0039] Figure 5 It is a cross-sectional view of a fully automatic nucleic acid extraction device of the present invention when the base slides to the second slide groove located above;

[0040] Figure 6 It is a cross-sectional view of a fully automatic nucleic acid extraction device of the present invention when the base slides to the second slide groove located below;

[0041] Figure 7It is a cross-sectional view of a fully automatic nucleic acid extraction device of the present invention when the base slides to the first slide groove;

[0042] Figure 8 A stereoscopic view of a wheel disc in a fully automatic nucleic acid extraction device of the present invention;

[0043] Fig. 9 It is a cross-sectional view of a fully automatic nucleic acid extraction device of the present invention when the wheel disc is in a horizontal section;

[0044] Fig.10 It is a cross-sectional view of a fully automatic nucleic acid extraction device of the present invention when the wheel is in a connecting section;

[0045] Fig.11 It is a partial cross-sectional view of a base in a fully automatic nucleic acid extraction device of the present invention;

[0046] Fig.12 It is a cross-sectional view of an elliptical stopper in a fully automatic nucleic acid extraction device of the present invention;

[0047] Fig.13 It is a cross-sectional view of a stopper of a Lello triangle in a fully automatic nucleic acid extraction device of the present invention.

[0048] Among them: 1. body; 101. extraction chamber; 102. first slide groove; 103. second slide groove; 1031. horizontal section; 1032. connecting section; 104. installation chamber; 2. cabinet door; 3. base; 31. support seat; 32. sliding frame; 33. connecting bolt; 4. guide mechanism; 41. sliding seat; 42. connecting rod; 43. wheel; 431. fixed rod; 432. movable disk; 433. turntable; 434. sliding rod; 435. spring; 5. limiter. DETAILED DESCRIPTION

[0049] The following will be combined with the specific implementation of the present invention to clearly and completely describe the technical solution in the present invention. Obviously, the described implementation is only a part of the implementation of the present invention, not all of the implementation. Based on the implementation of the present invention, all other implementations obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0050] like Figure 1-13 As shown, a fully automatic nucleic acid extraction device of the present invention includes a body 1, a cabinet door 2, a base 3, a guide mechanism 4 and a limiter 5.

[0051] An extraction chamber 101 is provided on the peripheral side of the body 1 , and the cabinet door 2 is detachably fixed to the body 1 and seals the extraction chamber 101 , so that the extraction chamber 101 forms a relatively sealed and stable environment, thereby being suitable for nucleic acid extraction operations.

[0052] The base 3 is used to fix the test tube rack carrying the test sample and the corresponding detection reagent. When performing the nucleic acid extraction operation, the base 3 needs to be in the extraction chamber 101. However, the space in the extraction chamber 101 is relatively small. When the test tube rack is installed on the base 3, its operation is relatively inconvenient, which affects the operating efficiency of the nucleic acid extraction device. For this reason, the base 3 is set to be movably connected with the body 1. When the test tube rack needs to be installed on the base 3, the base 3 can be extended out of the extraction chamber 101, that is, the base 3 is moved to the outside of the body 1 before the test tube rack is installed.

[0053] The base 3 is used to fix the test tube rack carrying samples and detection reagents, which needs to be kept horizontal when moving into or out of the extraction chamber 101. The guide mechanism 4 is used to maintain the horizontal state of the base 3. The guide mechanism 4 includes a slide 41, a connecting rod 42 and a wheel 43. The slide 41 is slidably set in the extraction chamber 101. The sliding direction of the slide 41 is the same as the opening direction of the extraction chamber 101. The two ends of the connecting rod 42 are rotatably set on the slide 41 and the base 3 respectively. A group of connecting rods 42 are respectively set at both ends of the base 3, and each group of connecting rods 42 is provided with multiple connecting rods. The lengths of the multiple connecting rods 42 are the same, and the multiple connecting rods 42 are parallel and spaced apart. Figure 3 and Figure 4 As shown, two connecting rods 42 located at the same end of the base 3 enclose a parallelogram shape. As long as the slide 41 and the body 1 do not rotate, the base 3 will always be in a horizontal state, thereby avoiding the problem of reagents in the test tube rack being spilled due to the tilt of the base 3.

[0054] like Figure 2 As shown, a first slide groove 102 and at least one second slide groove 103 are provided on the side wall of the extraction chamber 101, and an end of the second slide groove 103 away from the cabinet door 2 is connected to the first slide groove 102, and an end of the second slide groove 103 close to the cabinet door 2 is located above an end of the first slide groove 102 close to the cabinet door 2, and a wheel 43 is fixedly or rotatably arranged on the connecting rod 42, and is slidably or rollingly connected to the first slide groove 102 and the second slide groove 103, as shown in FIG. Figure 5-Figure 7 As shown, during the sliding process of the slide 41 and the base 3, the wheel 43 can be moved out of the extraction chamber 101 along the path of the first slide groove 102, or the wheel 43 can be moved out of the extraction chamber 101 along the path of one of the second slide grooves 103, so that the base 3 moved to the outside of the body 1 is at different heights to adapt to different usage conditions or different operators.

[0055] It is preferred that the first slide groove 102 is arranged in the horizontal direction, that is, the length direction of the first slide groove 102 is the same as the opening direction of the extraction chamber 101, so that the base 3 can be directly moved out of the extraction chamber 101 in the horizontal direction.

[0056] like Figure 2 As shown, the second sliding groove 103 includes a horizontal section 1031 and a connecting section 1032. A plurality of horizontal sections 1031 are parallel to and spaced from the first sliding groove 102. Both ends of the connecting section 1032 are respectively connected to the end of the horizontal section 1031 away from the cabinet door 2 and the first sliding groove 102. The connecting section 1032 is arc-shaped, and the centers of a plurality of connecting sections 1032 are the same, while the radii and arc lengths are different. With this structural design, each second sliding groove 103 is in a factory shape, enabling the roulette 43 to first select a moving path at the initial stage of the movement of the base 3 and then move horizontally along a stable path, which helps to improve the moving stability of the base 3.

[0057] During the use of the nucleic acid extraction device, vibrations will occur. To prevent this vibration from affecting the position of the base 3, it is preferably to set a certain sliding resistance between the sliding seat 41 and the body 1 so that the sliding seat 41 cannot slide easily.

[0058] As Figure 2 shown, there are two second sliding grooves 103. The connecting section 1032 away from the cabinet door 2 is connected to the end of the first sliding groove 102 away from the cabinet door 2. With the design of the connecting section 1032, when the roulette 43 moves into the upper horizontal section 1031, the end of the connecting rod 42 close to the roulette 43 is located above the end of the connecting rod 42 away from the roulette 43. When the roulette 43 moves into the lower horizontal section 1031 or the first sliding groove 102, the end of the connecting rod 42 close to the roulette 43 is located below the end of the connecting rod 42 away from the roulette 43. Since a certain sliding resistance is set between the sliding seat 41 and the body 1, the sliding resistance of the sliding seat 41 in the body 1 is greater than the moving resistance of the roulette 43 in the second sliding groove 103 and the rotating resistance of the connecting rod 42. When a small pulling force is applied to the base 3 in the direction of moving out of the extraction chamber 101, the roulette 43 will first move from the left end of the first sliding groove 102 into the left connecting section 1032, and then the roulette 43 will be stuck Figure 2 at the position where the roulette 43 is located, and the base 3 cannot be moved out of the extraction chamber 101, achieving a certain anti-theft effect.

[0059] When the position of the base 3 moved out of the extraction chamber 101 rises, it will cause a lateral offset of the center of gravity of the nucleic acid extraction device. To reduce the offset amount of the center of gravity and enable the nucleic acid extraction device to always maintain a stable state, as Figure 2 shown, the distance between the ends of a plurality of horizontal sections 1031 close to the cabinet door 2 and the cabinet door 2 gradually decreases from top to bottom, and the distance between the end of the horizontal section 1031 close to the cabinet door 2 and the cabinet door 2 is greater than the distance between the end of the first sliding groove 102 close to the cabinet door 2 and the cabinet door 2.

[0060] As Figure 8As shown, the wheel 43 includes a fixed rod 431, a movable plate 432, two rotating plates 433, a sliding rod 434 and a spring 435. The two ends of the fixed rod 431 are rotatably set on the two connecting rods 42 of the same group respectively. The movable plate 432 is fixedly set on the fixed rod 431 and slidably set in the first slide groove 102 and the second slide groove 103. The two rotating plates 433 are both rotatably set on the movable plate 432 and are rollingly connected with the upper and lower sides in the first slide groove 102 and the horizontal section 1031 respectively. The two rotating plates 433 are rollingly connected, and the outer diameter of the movable plate 432 is less than 2 times the outer diameter of the rotating plates 433. Since the fixed rod 431 is rotatably connected to the two connecting rods 42, it is also combined with the two connecting rods 42 and the sliding seat 41 to form a parallelogram structure. The length direction of the fixed rod 431 remains unchanged during the rotation of the connecting rod 42. Fig. 9 As shown, when the wheel 43 moves into the horizontal section 1031, the top side of the upper turntable 433 is always in contact with and rollingly connected to the top side of the horizontal section 1031, the bottom side of the lower turntable 433 is always in contact with and rollingly connected to the bottom side of the horizontal section 1031, and the bottom side of the upper turntable 433 is always in contact with and rollingly connected to the top side of the lower turntable 433, so that the wheel 43 can move stably in the horizontal section 1031, avoiding the problem of the wheel 43 shaking up and down during the movement of the base 3. The moving state of the wheel 43 in the first slide groove 102 is the same.

[0061] The sliding rod 434 is slidably disposed on the moving plate 432, and is preferably dumbbell-shaped. The spring 435 is fixedly disposed on the moving plate 432, and one end of the spring 435 abuts against one end of the sliding rod 434, so that the other end of the sliding rod 434 abuts against a side of the connecting section 1032 close to the cabinet door 2, as shown in FIG. Fig.10 As shown, when the wheel 43 moves into the connecting section 1032 , the elastic force of the spring 435 causes the sliding rod 434 to always press against the side of the connecting section 1032 close to the cabinet door 2 , thereby allowing the wheel 43 to move stably in the connecting section 1032 .

[0062] In some embodiments, in order to improve the movement stability of the wheel 43 in the connecting end 1032, two sliding rods 434 and two springs 435 can also be set on each movable disk 432, the sliding rods 434 and the springs 435 correspond to each other one by one, and the two sliding rods 434 are respectively abutted against the side of the connecting section 1032 close to the cabinet door 2 and the side of the connecting section 1032 away from the cabinet door 2, but this structural design will have a certain impact on the initial position of the base 3.

[0063] In order to realize the nucleic acid extraction operation, a pipetting module for pipetting samples and detection reagents and a driving component for driving the pipetting module to move up and down are also required to be arranged in the body 1. The pipetting module is installed above the extraction chamber 101. In order to realize the up and down movement control of the pipetting module, it is preferred to open an installation cavity 104 on the side of the body 1 away from the extraction chamber 101, so that the installation cavity 104 is connected to the extraction chamber 101, and the driving component of the pipetting module is installed in the installation cavity 104.

[0064] The stopper 5 is used to limit and fix the base 3 during the nucleic acid extraction process to prevent the base 3 from moving during the operation of the nucleic acid extraction device and affecting the detection result.

[0065] The design of the installation cavity 104 makes the length of the nucleic acid extraction device in the direction in which the extraction cavity 101 is opened larger. In order to achieve a lightweight design of the device, the space occupied by other structures in this direction should be appropriately reduced.

[0066] In order to meet the space occupied by the stopper 5 in the direction of the extraction chamber 101, the base 3 is configured to include a support seat 31, a sliding frame 32 and a connecting bolt 33, such as Fig.11 As shown, the support seat 31 is rotatably connected to the connecting rod 42, the sliding frame 32 is slidably arranged on the bottom side of the support seat 31, and the sliding direction of the sliding frame 32 is parallel to the opening direction of the first sliding groove 102, and the connecting bolt 33 is connected to the support seat 31 through threaded cooperation. When the connecting bolt 33 is tightened, the connecting bolt 33 can be engaged with the sliding frame 32, thereby fixing the sliding frame 32 and the support seat 31, and when the connecting bolt 33 is loosened, the connecting bolt 33 can be separated from the sliding frame 32, so that the sliding frame 32 can slide on the support seat 31; the limiter 5 is rotatably arranged on the body 1, and the limiter 5 is preferably made of materials such as rubber with large friction force. The limiter 5 is abutted and rollingly connected to one end of the sliding frame 32 close to the connecting rod 42, and the cross-section of the limiter 5 is non-circular, such as Fig.12As shown, when the stopper 5 is elliptical and the base 3 is located at the initial position in the extraction chamber 101, the support seat 31 and the sliding frame 32 are fixed by the connecting bolts 33, so that the side of the stopper 5 parallel to its long axis is against the sliding frame 32. At this time, if the base 3 is pulled upward, the stopper 5 will rotate at a certain angle, thereby forcing the sliding frame 32 to deviate to the right. Since the sliding frame 32 is fixedly connected to the support seat 31 at this time, the sliding frame 32 cannot deviate, so that the base 3 cannot be pulled upward. When the connecting bolts 33 are loosened, the stopper 5 can allow the sliding frame 32 to move to the right, so that the base 3 can be pulled upward, thereby realizing the limit of the base 3 by the stopper 5. Since the stopper 5 is not arranged on the side of the base 3 along the direction of the extraction chamber 101, it is helpful to improve the lightweight design of the nucleic acid extraction device; wherein, the initial position of the base 3 refers to the position of the base 3 when the nucleic acid extraction device is running.

[0067] An elliptical stopper 5 cannot realize the reset function of the sliding frame 32. After the base 3 is pushed back into the extraction chamber 101, the sliding frame 32 needs to be manually adjusted, which is inconvenient. Therefore, two stoppers 5 are provided, and the two stoppers 5 are respectively abutted and rolled on the two ends of the sliding frame 32, and the cross section of the stopper 5 is a Lello triangle, such as Fig.13 As shown, when the connecting bolt 33 is tightened and the base 3 is pulled upward, the base 3 still does not move, and when the connecting bolt 33 is loosened and the base 3 is pulled upward, the left limiter 5 can be driven to rotate counterclockwise, and the right limiter 5 can be driven to rotate clockwise, but the rotation angles of the two limiters 5 are the same. Since the Lello triangle has equal width and symmetry, the distance between the two limiters 5 will not change after the two limiters 5 rotate at the same angle, but the sliding frame 32 will be allowed to slide back and forth in a wave-like path, so that when the base 3 moves downward, the sliding frame 32 can be moved to the initial position to achieve rapid resetting of the sliding frame 32, which is convenient for subsequent other operations.

[0068] The method of using a fully automatic nucleic acid extraction device of the present invention is as follows:

[0069] S1, multiple test tubes containing 200 μl of the sample to be tested, 3-5 μl of the lysis reagent, 15-25 μl of the magnetic bead reagent, 300-800 μl of the washing solution and 20-50 μl of the elution reagent are sequentially installed on the test tube rack.

[0070] S2, open the cabinet door 2 and loosen the connecting bolts 33.

[0071] S3, pull the base 3 outward, and use the control of the pulling force to move the wheel 43 to the required first slide groove 102 or second slide groove 103 until the base 3 moves to the outside of the body 1.

[0072] S4, installing the test tube rack on the base 3, and pushing the base 3 back into the body 1.

[0073] S5, tighten the connecting bolts 33, close the cabinet door 2, and start the nucleic acid extraction device to perform the nucleic acid extraction operation.

[0074] In some embodiments, two second chutes 103 are provided, and the distance between the two horizontal sections 1031 and the distance between the lower horizontal section 1031 and the first chutes 102 are both 80 mm, so that the height adjustment range of the base 3 extending out of the extraction chamber 101 is 160 mm, so that the nucleic acid extraction device can adapt to most operating conditions and the use requirements of different operators; the weight of the base 3 accounts for 5-10% of the total weight of the nucleic acid extraction device, and the length of the base 3 along the direction in which the extraction chamber 101 is opened is 120-180 mm. Figure 2 As shown, the lateral spacing between the right ends of the two horizontal sections 1031 is 20-40 mm, and the lateral spacing between the right end of the lower horizontal section 1031 and the right end of the first slide groove 102 is also 20-40 mm, so as to avoid affecting the balance of the nucleic acid extraction device when the base 3 is pulled out.

[0075] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A fully automatic nucleic acid extraction device, characterized in that: It comprises a machine body (1), a cabinet door (2), a base (3) and a guide mechanism (4), wherein: An extraction chamber (101) is provided on the peripheral side of the machine body (1); The cabinet door (2) is detachably fixed to the machine body (1) and blocks the extraction chamber (101); a first slide groove (102) and at least one second slide groove (103) are provided on the side wall inside the extraction chamber (101); an end of the second slide groove (103) away from the cabinet door (2) is connected to the first slide groove (102), and an end of the second slide groove (103) close to the cabinet door (2) is located above an end of the first slide groove (102) close to the cabinet door (2); The guide mechanism (4) comprises a slide seat (41), a connecting rod (42) and a wheel disc (43); the slide seat (41) is slidably arranged in the extraction chamber (101); the two ends of the connecting rod (42) are rotatably arranged on the slide seat (41) and the base (3), respectively; a group of connecting rods (42) are respectively arranged at the two ends of the base (3), and each group of connecting rods (42) is provided with a plurality of connecting rods, and the plurality of connecting rods (42) are arranged in parallel and at intervals; the wheel disc (43) is fixedly or rotatably arranged on the connecting rod (42), and is slidably or rollingly connected to the first slide groove (102) and the second slide groove (103).

2. A fully automatic nucleic acid extraction device as claimed in claim 1, characterized in that: The first slide groove (102) is arranged along the horizontal direction; The second slide groove (103) comprises a horizontal section (1031) and a connecting section (1032); a plurality of the horizontal sections (1031) and the first slide groove (102) are arranged in parallel and at intervals; two ends of the connecting section (1032) are respectively connected to an end of the horizontal section (1031) away from the cabinet door (2) and the first slide groove (102); the connecting section (1032) is arc-shaped, and the center of the circle of the plurality of connecting sections (1032) is the same, but the radius and arc length are different.

3. A fully automatic nucleic acid extraction device as claimed in claim 2, characterized in that: The sliding resistance of the sliding seat (41) in the machine body (1) is greater than the moving resistance of the wheel disc (43) in the second sliding groove (103) and the rotation resistance of the connecting rod (42); The second slide groove (103) is provided with two, the connecting section (1032) away from the cabinet door (2) is connected with the end of the first slide groove (102) away from the cabinet door (2), and when the wheel disc (43) moves to the horizontal section (1031) located above, the end of the connecting rod (42) close to the wheel disc (43) is located above the end of the connecting rod (42) away from the wheel disc (43), and when the wheel disc (43) moves to the horizontal section (1031) located below or the first slide groove (102), the end of the connecting rod (42) close to the wheel disc (43) is located below the end of the connecting rod (42) away from the wheel disc (43).

4. A fully automatic nucleic acid extraction device as claimed in claim 3, characterized in that: The wheel disc (43) comprises a fixed rod (431), a movable disc (432) and two rotating discs (433), wherein: The two ends of the fixing rod (431) are respectively rotatably arranged on the two connecting rods (42) of the same group; The movable plate (432) is fixedly arranged on the fixed rod (431) and is slidably arranged in the first sliding groove (102) and the second sliding groove (103); The two rotating disks (433) are both rotatably arranged on the movable disk (432) and are respectively rollingly connected to the first slide groove (102) and the upper and lower sides of the horizontal section (1031). The two rotating disks (433) are rollingly connected to each other, and the outer diameter of the movable disk (432) is less than twice the outer diameter of the rotating disk (433).

5. A fully automatic nucleic acid extraction device as claimed in claim 4, characterized in that: The wheel disc (43) further comprises a sliding rod (434) and a spring (435), wherein: The sliding rod (434) is slidably disposed on the moving plate (432); The spring (435) is fixedly arranged on the movable plate (432), and one end of the spring is abutted against one end of the sliding rod (434), so that the other end of the sliding rod (434) is abutted against a side of the connecting section (1032) close to the cabinet door (2).

6. A fully automatic nucleic acid extraction device as claimed in claim 5, characterized in that: Two sliding rods (434) and two springs (435) are provided on each of the movable plates (432), the sliding rods (434) and the springs (435) corresponding to each other one by one, and the two sliding rods (434) are respectively abutted against a side of the connecting section (1032) close to the cabinet door (2) and a side of the connecting section (1032) away from the cabinet door (2).

7. A fully automatic nucleic acid extraction device as claimed in claim 1, characterized in that: A mounting cavity (104) is provided on a side of the machine body (1) away from the extraction cavity (101); the mounting cavity (104) is communicated with the extraction cavity (101) and is used for mounting a driving component of a liquid transfer module.

8. A fully automatic nucleic acid extraction device as claimed in claim 7, characterized in that: It also includes a stopper (5), the base (3) includes a support seat (31), a sliding frame (32) and a connecting bolt (33), wherein: The support seat (31) is rotatably connected to the connecting rod (42); The sliding frame (32) is slidably arranged on the bottom side of the supporting seat (31), and its sliding direction is parallel to the opening direction of the first sliding groove (102); The connecting bolt (33) is connected to the supporting seat (31) through threaded engagement, and can be snap-fitted with the sliding frame (32); The stopper (5) is rotatably arranged on the machine body (1) and is in contact with and rolling connection with an end of the sliding frame (32) close to the connecting rod (42). The cross section of the stopper (5) is non-circular.

9. A fully automatic nucleic acid extraction device as claimed in claim 8, characterized in that: Two limiters (5) are provided, and the two limiters (5) are respectively abutted against and rollingly connected to the two ends of the sliding frame (32), and the cross section of the limiter (5) is a Lello triangle.

10. A fully automatic nucleic acid extraction device as claimed in claim 2, characterized in that: The distance between one end of the plurality of horizontal sections (1031) close to the cabinet door (2) and the cabinet door (2) gradually decreases from top to bottom, and the distance between one end of the horizontal section (1031) close to the cabinet door (2) and the cabinet door (2) is greater than the distance between one end of the first sliding groove (102) close to the cabinet door (2) and the cabinet door (2).

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