A fully automated nucleic acid extraction device

Abstract

This invention relates to the field of medical device technology and proposes a fully automated nucleic acid extraction device, including a body, a cabinet door, a base, and a guiding mechanism. An extraction cavity is formed on the periphery of the body; a first sliding groove and at least one second sliding groove are formed on the side wall of the extraction cavity; the guiding mechanism includes a slide block, a connecting rod, and a wheel. The slide block is slidably disposed within the extraction cavity; the two ends of the connecting rod are rotatably disposed on the slide block and the base, respectively; the wheel is fixedly or rotatably disposed on the connecting rod and is slidably or rollably connected to the first and second sliding grooves. By setting the slide block and connecting rod, and arranging multiple connecting rods parallel and spaced apart, this invention allows the base to slide smoothly into or out of the extraction cavity, facilitating the installation of test tube racks. By setting the first and second sliding grooves, the base sliding out of the extraction cavity can be fixed at different heights, making this nucleic acid extraction device adaptable to different operating conditions.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, and in particular to a fully automated nucleic acid extraction device. Background Technology

[0002] Nucleic acid extraction equipment is a type of instrument specifically designed to separate and purify nucleic acids (DNA or RNA) from biological samples (such as blood, tissues, cells, viruses, etc.). It typically integrates advanced biotechnology and automation technology to complete the nucleic acid extraction process in a highly efficient, accurate, and reproducible manner.

[0003] The invention disclosed in CN115386457B is a nucleic acid extractor, which includes a reagent strip assembly, a pipetting module, a magnetic separation assembly, and a heating assembly. The reagent strip assembly includes a reagent strip base and reagent strips. Multiple reagent strips are arranged along the length of the reagent strip base. Each reagent strip includes a heating tube and a magnetic separation tube. The pipetting module is located above the reagent strip base and includes a pipetting pump assembly, a first driving component, and a second driving component. The first driving component drives the pipetting pump assembly to move up and down, and the second driving component drives the pipetting pump assembly to move back and forth along the length of the reagent strip. The magnetic separation assembly includes a magnet assembly and a fourth driving component. The magnet assembly is located below the magnetic separation tube, and the fourth driving component drives the magnet assembly to move up and down. The heating assembly is located below the heating tube.

[0004] In the above technical solution, in order to provide a safe and stable operating environment for nucleic acid extraction, the base plate used to fix the test tube rack is placed inside the outer shell. However, the operating space inside the outer shell is relatively small, making it inconvenient to connect and fix the test tube rack to the base plate. This not only reduces the installation efficiency of the test tube rack, but also makes it difficult to ensure the firmness of the test tube rack. Summary of the Invention

[0005] In view of this, the present invention proposes a fully automated nucleic acid extraction device that can quickly and securely install the test tube rack on the base, thereby improving the operating efficiency of the nucleic acid extraction device.

[0006] The technical solution of this invention is implemented as follows: This invention provides a fully automated nucleic acid extraction device, including a body, a cabinet door, a base, and a guiding mechanism, wherein,

[0007] An extraction cavity is provided on the periphery of the body;

[0008] The cabinet door is detachably fixed to the machine body and blocks the extraction cavity. A first sliding groove and at least one second sliding groove are provided on the side wall of the extraction cavity. The end of the second sliding groove away from the cabinet door is connected to the first sliding groove, and the end of the second sliding groove near the cabinet door is located above the end of the first sliding groove near the cabinet door.

[0009] The guiding mechanism includes a slide, a connecting rod, and a wheel. The slide is slidably disposed within the extraction cavity. The two ends of the connecting rod are rotatably disposed on the slide and the base, respectively. A set of connecting rods is disposed on each end of the base. Each set of connecting rods consists of multiple rods, which are parallel and spaced apart. The wheel is fixedly or rotatably disposed on the connecting rod and is slidably or rollably connected to the first and second sliding grooves.

[0010] Based on the above technical solutions, preferably, the first chute is arranged in a horizontal direction;

[0011] The second slide rail includes a horizontal section and a connecting section. The multiple horizontal sections are parallel to and spaced apart from the first slide rail. The two ends of the connecting section are respectively connected to the end of the horizontal section away from the cabinet door and the first slide rail. The connecting section is arc-shaped, and the multiple connecting sections have the same center but different radii and arc lengths.

[0012] More preferably, the sliding resistance of the slide block in the machine body is greater than the moving resistance of the wheel in the second slide groove and the rotational resistance of the connecting rod;

[0013] The second slide has two sections. The connecting section away from the cabinet door is connected to the end of the first slide away from the cabinet door. When the wheel moves to the upper horizontal section, the end of the connecting rod near the wheel is above the end of the connecting rod away from the wheel. When the wheel moves to the lower horizontal section or the first slide, the end of the connecting rod near the wheel is below the end of the connecting rod away from the wheel.

[0014] More preferably, the wheel includes a fixed rod, a movable disk, and two turntables, wherein,

[0015] The two ends of the fixed rod are respectively rotatably mounted on the two connecting rods in the same group;

[0016] The movable disk is fixedly mounted on the fixed rod and slidably mounted within the first and second slide grooves;

[0017] Both turntables are rotatably mounted on the movable disk and are respectively rolledly connected to the upper and lower sides of the first slide groove and the horizontal section. The two turntables are rolledly connected to each other, and the outer diameter of the movable disk is less than twice the outer diameter of the turntables.

[0018] More preferably, the wheel further includes a sliding rod and a spring, wherein,

[0019] The sliding rod is slidably mounted on the movable disk;

[0020] The spring is fixedly mounted on the movable disk, with one end abutting against one end of the sliding rod, so that the other end of the sliding rod abuts against the side of the connecting section near the cabinet door.

[0021] More preferably, each of the movable disks is provided with two sliding rods and two springs, the sliding rods and the springs are in one-to-one correspondence, and the two sliding rods respectively abut against the side of the connecting section near the cabinet door and the side of the connecting section away from the cabinet door.

[0022] Based on the above technical solutions, preferably, an installation cavity is provided on the side of the machine body away from the extraction cavity, and the installation cavity is connected to the extraction cavity for installing the driving component of the pipetting module.

[0023] A further preferred embodiment includes a limiter, wherein the base comprises a support, a sliding frame, and connecting bolts, wherein...

[0024] The support base is rotatably connected to the connecting rod;

[0025] The sliding frame is slidably disposed on the bottom side of the support base, and its sliding direction is parallel to the opening direction of the first sliding groove;

[0026] The connecting bolts are threaded onto the support base and can engage with the sliding frame.

[0027] The limiter is rotatably mounted on the machine body and abuts against and rolls against the end of the sliding frame near the connecting rod. The cross-section of the limiter is non-circular.

[0028] More preferably, there are two limiters, which are respectively abutted against and rolled to the two ends of the sliding frame, and the cross-section of the limiters is a Reuleaux triangle.

[0029] Based on the above technical solutions, preferably, the distance between the end of the plurality of horizontal segments near the cabinet door and the cabinet door gradually decreases from top to bottom, and the distance between the end of the horizontal segment near the cabinet door and the cabinet door is greater than the distance between the end of the first slide groove near the cabinet door and the cabinet door.

[0030] The fully automated nucleic acid extraction device of the present invention has the following advantages over the prior art:

[0031] (1) By setting up a slide and connecting rods, and setting multiple connecting rods in parallel and spaced apart, the base can be smoothly slid out or into the extraction chamber to facilitate the installation of the test tube rack. By setting up the first slide groove and the second slide groove, the base that has slid out of the extraction chamber can be fixed at different heights, so that this nucleic acid extraction device can be adapted to different working conditions.

[0032] (2) By setting a fixed rod, a movable disc and a wheel, the wheel is made to roll against the top and bottom sides of the first slide groove and the horizontal section. By setting a sliding rod and a spring, the sliding rod is made to slide against the side wall of the connecting section. This allows the wheel to move stably, thus ensuring the stability of the base's movement.

[0033] (3) By setting the base to include a support, a sliding frame and connecting bolts, and setting a limiter with a non-circular cross-section on the machine body, the movement of the base can be limited by the limiter against the sliding frame, and the space occupied by the nucleic acid extraction equipment along the opening direction of the extraction chamber can be reduced. Attached Figure Description

[0034] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0035] Figure 1 This is a perspective view of a fully automated nucleic acid extraction device according to the present invention;

[0036] Figure 2 This is a cross-sectional view of the second chute in a fully automated nucleic acid extraction device according to the present invention;

[0037] Figure 3 This is a perspective view of the guiding mechanism in a fully automated nucleic acid extraction device according to the present invention;

[0038] Figure 4 This is a cross-sectional view of a fully automated nucleic acid extraction device according to the present invention;

[0039] Figure 5 This is a cross-sectional view of the base of a fully automated nucleic acid extraction device of the present invention when it slides to the second slide groove located above.

[0040] Figure 6 This is a cross-sectional view of the base of a fully automated nucleic acid extraction device of the present invention when it slides to the second slide groove located below.

[0041] Figure 7This is a cross-sectional view of the base sliding into the first chute in a fully automated nucleic acid extraction device according to the present invention;

[0042] Figure 8 This is a perspective view of the rotary disk in a fully automated nucleic acid extraction device according to the present invention;

[0043] Figure 9 This is a cross-sectional view of the rotary disk in the horizontal section of a fully automated nucleic acid extraction device according to the present invention;

[0044] Figure 10 This is a cross-sectional view of the rotary disk in a fully automated nucleic acid extraction device according to the present invention, when the disk is in a connected segment.

[0045] Figure 11 This is a partial cross-sectional view of the base in a fully automated nucleic acid extraction device according to the present invention;

[0046] Figure 12 This is a cross-sectional view of the elliptical limiter in a fully automated nucleic acid extraction device according to the present invention;

[0047] Figure 13 This is a cross-sectional view of the limiter of the Reilly triangle in a fully automated nucleic acid extraction device of the present invention.

[0048] The components are as follows: 1. Body; 101. Extraction chamber; 102. First slide groove; 103. Second slide groove; 1031. Horizontal section; 1032. Connecting section; 104. Mounting chamber; 2. Cabinet door; 3. Base; 31. Support seat; 32. Sliding frame; 33. Connecting bolt; 4. Guide mechanism; 41. Slide seat; 42. Connecting rod; 43. Wheel; 431. Fixed rod; 432. Moving plate; 433. Turntable; 434. Sliding rod; 435. Spring; 5. Limiter. Detailed Implementation

[0049] The technical solutions of this invention will be clearly and completely described below with reference to specific embodiments. Obviously, the described embodiments are only a part of the embodiments of this invention, and not all of them. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention.

[0050] like Figure 1-13 As shown, the fully automated nucleic acid extraction device of the present invention includes a body 1, a cabinet door 2, a base 3, a guide mechanism 4, and a limiter 5.

[0051] An extraction chamber 101 is provided on the periphery of the machine body 1. The cabinet door 2 is detachably fixed to the machine body 1 and seals the extraction chamber 101, so that the extraction chamber 101 forms a relatively sealed and stable environment, which is suitable for nucleic acid extraction operation.

[0052] The base 3 is used to fix the test tube rack containing the test samples and corresponding test reagents. When performing nucleic acid extraction, the base 3 needs to be inside the extraction chamber 101. However, the space inside the extraction chamber 101 is relatively small, making it inconvenient to install the test tube rack on the base 3, thus affecting the operating efficiency of the nucleic acid extraction equipment. Therefore, the base 3 is configured to be movably connected to the body 1. When it is necessary to install the test tube rack on the base 3, the base 3 can extend out of the extraction chamber 101. That is, the base 3 is moved to the outside of the body 1 before the test tube rack is installed.

[0053] The base 3 is used to fix the test tube rack containing the sample and test reagents. It needs to remain horizontal when moving into or out of the extraction chamber 101. The guide mechanism 4 is used to maintain the horizontal state of the base 3. The guide mechanism 4 includes a slide 41, a connecting rod 42, and a wheel 43. The slide 41 is slidably disposed within the extraction chamber 101, and the sliding direction of the slide 41 is the same as the opening direction of the extraction chamber 101. The two ends of the connecting rod 42 are rotatably disposed on the slide 41 and the base 3, respectively. A set of connecting rods 42 is provided at each end of the base 3. Each set of connecting rods 42 has multiple rods, all of the same length, and the multiple connecting rods 42 are parallel and spaced apart. Figure 3 and Figure 4 As shown, two connecting rods 42 located at the same end of the base 3 form a parallelogram shape. As long as the slide 41 and the body 1 do not rotate, the base 3 will always be in a horizontal state, thereby avoiding the problem of reagents spilling from the test tube rack due to the tilt of the base 3.

[0054] like Figure 2 As shown, a first groove 102 and at least one second groove 103 are provided on the side wall of the extraction cavity 101. The end of the second groove 103 away from the cabinet door 2 is connected to the first groove 102, and the end of the second groove 103 near the cabinet door 2 is located above the end of the first groove 102 near the cabinet door 2. The wheel 43 is fixedly or rotatably mounted on the connecting rod 42 and is slidably or rollably connected with the first groove 102 and the second groove 103. Figures 5-7 As shown, during the sliding process of the slide block 41 and the base 3, the wheel 43 can be moved out of the extraction cavity 101 along the path of the first slide groove 102, or the wheel 43 can be moved out of the extraction cavity 101 along the path of one of the second slide grooves 103, so that the base 3 moved to the outside of the machine body 1 is at different heights to adapt to different working conditions or different operators.

[0055] Preferably, the first slide groove 102 is set in the horizontal direction, that is, the length direction of the first slide groove 102 is the same as the opening direction of the extraction cavity 101, so that the base 3 can be moved directly out of the extraction cavity 101 in the horizontal direction.

[0056] like Figure 2 As shown, the second chute 103 includes a horizontal section 1031 and a connecting section 1032. Multiple horizontal sections 1031 are parallel and spaced apart from the first chute 102. The two ends of the connecting section 1032 are respectively connected to the end of the horizontal section 1031 away from the cabinet door 2 and the first chute 102. The connecting section 1032 is arc-shaped, and the centers of multiple connecting sections 1032 are the same, while the radii and arc lengths are different. With this structural design, each second chute 103 is in a "factory" shape, enabling the roulette 43 to first select a moving path at the initial stage of the movement of the base 3 and then move horizontally along a smooth path, which helps to improve the moving stability of the base 3.

[0057] During the use of the nucleic acid extraction device, vibrations may occur. To prevent such vibrations from affecting the position of the base 3, it is preferable to set a certain sliding resistance between the sliding seat 41 and the body 1 so that the sliding seat 41 cannot slide easily.

[0058] As Figure 2 shown, there are two second chutes 103. The connecting section 1032 away from the cabinet door 2 is connected to the end of the first chute 102 away from the cabinet door 2. With the design of the connecting section 1032, when the roulette 43 moves into the upper horizontal section 1031, the end of the connecting rod 42 close to the roulette 43 is above the end of the connecting rod 42 away from the roulette 43. When the roulette 43 moves into the lower horizontal section 1031 or the first chute 102, the end of the connecting rod 42 close to the roulette 43 is below the end of the connecting rod 42 away from the roulette 43. Since a certain sliding resistance is set between the sliding seat 41 and the body 1, the sliding resistance of the sliding seat 41 in the body 1 is greater than the moving resistance of the roulette 43 in the second chute 103 and the rotating resistance of the connecting rod 42. When a small pulling force is applied to the base 3 in the direction of moving out of the extraction chamber 101, the roulette 43 will first move from the left end of the first chute 102 into the left connecting section 1032, and then the roulette 43 will be stuck in Figure 2 the position where the roulette 43 is located, and the base 3 cannot be moved out of the extraction chamber 101, achieving a certain anti-theft effect.

[0059] When the position of the base 3 moved out of the extraction chamber 101 rises, it will cause a lateral offset of the center of gravity of the nucleic acid extraction device. To reduce the offset amount of the center of gravity and enable the nucleic acid extraction device to always maintain a stable state, as Figure 2 shown, the distance between the end of multiple horizontal sections 1031 close to the cabinet door 2 and the cabinet door 2 gradually decreases from top to bottom, and the distance between the end of the horizontal section 1031 close to the cabinet door 2 and the cabinet door 2 is greater than the distance between the end of the first chute 102 close to the cabinet door 2 and the cabinet door 2.

[0060] As Figure 8As shown, the wheel 43 includes a fixed rod 431, a movable disk 432, two turntables 433, a sliding rod 434, and a spring 435. The two ends of the fixed rod 431 are rotatably mounted on two connecting rods 42 in the same group. The movable disk 432 is fixedly mounted on the fixed rod 431 and slidably mounted within the first slide groove 102 and the second slide groove 103. Both turntables 433 are rotatably mounted on the movable disk 432 and are respectively rolledly connected to the upper and lower sides of the first slide groove 102 and the horizontal section 1031. The two turntables 433 are rolledly connected to each other, and the outer diameter of the movable disk 432 is less than twice the outer diameter of the turntables 433. Since the fixed rod 431 is rotatably connected to both connecting rods 42, it also forms a parallelogram shape structure with the two connecting rods 42 and the slide block 41. The length direction of the fixed rod 431 remains unchanged during the rotation of the connecting rods 42. Figure 9 As shown, when the wheel 43 moves into the horizontal section 1031, the top side of the upper turntable 433 is always in contact with and rolls in connection with the top side of the horizontal section 1031, and the bottom side of the lower turntable 433 is always in contact with and rolls in connection with the bottom side of the horizontal section 1031. In addition, the bottom side of the upper turntable 433 and the top side of the lower turntable 433 are always in contact with and roll in connection, so that the wheel 43 can move stably within the horizontal section 1031, avoiding the problem of the wheel 43 shaking up and down during the movement of the base 3. The movement state of the wheel 43 within the first slide groove 102 is the same.

[0061] The sliding rod 434 is slidably mounted on the movable disk 432, preferably in the shape of a dumbbell. A spring 435 is fixedly mounted on the movable disk 432, with one end abutting against one end of the sliding rod 434, so that the other end of the sliding rod 434 abuts against the side of the connecting section 1032 near the cabinet door 2. Figure 10 As shown, when the wheel 43 moves into the connecting section 1032, the elastic force of the spring 435 causes the sliding rod 434 to always press against the side of the connecting section 1032 near the cabinet door 2, so that the wheel 43 can move stably within the connecting section 1032.

[0062] In some embodiments, in order to improve the stability of the movement of the wheel 43 within the connecting end 1032, two sliding rods 434 and two springs 435 can be provided on each moving disk 432. The sliding rods 434 and springs 435 correspond one-to-one, and the two sliding rods 434 respectively abut against the side of the connecting section 1032 near the cabinet door 2 and the side of the connecting section 1032 away from the cabinet door 2. However, this structural design will have a certain impact on the initial position of the base 3.

[0063] In order to realize the nucleic acid extraction operation, the body 1 also needs to be equipped with a pipetting module for transferring samples and test reagents, as well as a driving component for driving the pipetting module to move up and down. The pipetting module is installed above the extraction chamber 101. In order to realize the up and down movement control of the pipetting module, it is preferable to open an installation cavity 104 on the side of the body 1 away from the extraction chamber 101, so that the installation cavity 104 is connected to the extraction chamber 101, and the driving component of the pipetting module is installed in the installation cavity 104.

[0064] The limiter 5 is used to limit and fix the base 3 during the nucleic acid extraction process, so as to prevent the base 3 from moving during the operation of the nucleic acid extraction equipment and affecting the test results.

[0065] The design of the mounting cavity 104 results in a relatively large length of the nucleic acid extraction device in the direction of the extraction cavity 101. In order to achieve a lightweight design of the device, the space occupied by other structures in this direction should be appropriately reduced.

[0066] To accommodate the space occupied by the limiter 5 in the opening direction of the extraction cavity 101, the base 3 is configured to include a support 31, a sliding frame 32, and connecting bolts 33, as shown below. Figure 11 As shown, the support base 31 is rotatably connected to the connecting rod 42. The sliding frame 32 is slidably disposed on the bottom side of the support base 31, and the sliding direction of the sliding frame 32 is parallel to the opening direction of the first slide groove 102. The connecting bolt 33 is threadedly connected to the support base 31. When the connecting bolt 33 is tightened, it can engage with the sliding frame 32, thereby fixing the sliding frame 32 and the support base 31. When the connecting bolt 33 is loosened, it can disengage from the sliding frame 32, allowing the sliding frame 32 to slide on the support base 31. The limiter 5 is rotatably disposed on the machine body 1. The limiter 5 is preferably made of a material with high friction, such as rubber. The limiter 5 abuts against and rolls with the end of the sliding frame 32 near the connecting rod 42. The cross-section of the limiter 5 is non-circular. Figure 12As shown, when the limiter 5 is elliptical and the base 3 is in its initial position within the extraction chamber 101, the support 31 and the sliding frame 32 are fixed using the connecting bolt 33, so that the side of the limiter 5 parallel to its long axis abuts against the sliding frame 32. If the base 3 is pulled upwards at this time, the limiter 5 will rotate at a certain angle, thus causing the sliding frame 32 to shift to the right. Since the sliding frame 32 is fixedly connected to the support 31, it cannot shift, thus preventing the base 3 from being pulled upwards. However, when the connecting bolt 33 is loosened, the limiter 5 allows the sliding frame 32 to move to the right, allowing the base 3 to be pulled upwards, thereby limiting the position of the base 3 by the limiter 5. Because the limiter 5 is not located on the side of the base 3 along the opening direction of the extraction chamber 101, it helps to improve the lightweight design of the nucleic acid extraction equipment. The initial position of the base 3 refers to the position of the base 3 during operation of the nucleic acid extraction equipment.

[0067] An elliptical limiter 5 cannot achieve the reset function of the sliding frame 32. After pushing the base 3 back into the extraction cavity 101, the sliding frame 32 still needs to be manually adjusted, which is inconvenient. Therefore, two limiters 5 are provided, so that the two limiters 5 are respectively abutted and rolled on both ends of the sliding frame 32, and the cross-section of the limiter 5 is a Reichstag triangle, as shown. Figure 13 As shown, when the connecting bolt 33 is tightened and the base 3 is pulled upward, the base 3 will not move. However, when the connecting bolt 33 is loosened and the base 3 is pulled upward, the left limiter 5 can be rotated counterclockwise and the right limiter 5 can be rotated clockwise. However, the rotation angles of the two limiters 5 are the same. Since the Reilly triangle has equal width and symmetry, the distance between the two limiters 5 will not change after they rotate by the same angle. However, the sliding frame 32 will slide back and forth in a wave-like path. Thus, when the base 3 moves downward, the sliding frame 32 can be moved to the initial position to achieve a quick reset of the sliding frame 32, which is convenient for subsequent operations.

[0068] The method of using the fully automated nucleic acid extraction device of the present invention is as follows:

[0069] S1. Multiple test tubes, each containing 200 μl of the sample to be tested, 3-5 μl of lysis reagent, 15-25 μl of magnetic bead reagent, 300-800 μl of washing solution, and 20-50 μl of elution reagent, are sequentially mounted on a test tube rack.

[0070] S2, open cabinet door 2 and loosen connecting bolt 33.

[0071] S3, pull the base 3 outward, and use the control of the pulling force to move the wheel 43 into the desired first slide 102 or second slide 103 until the base 3 moves to the outside of the body 1.

[0072] S4, install the test tube rack on the base 3, and push the base 3 back into the machine body 1.

[0073] S5, tighten the connecting bolt 33, close the cabinet door 2, and start the nucleic acid extraction equipment to perform nucleic acid extraction.

[0074] In some embodiments, two second slide grooves 103 are provided, the distance between the two horizontal segments 1031 and the distance between the lower horizontal segment 1031 and the first slide groove 102 are both 80mm, so that the height adjustment range of the base 3 extending out of the extraction cavity 101 is 160mm, thereby enabling the nucleic acid extraction device to adapt to most operating conditions and the needs of different operators; the weight of the base 3 accounts for 5-10% of the total weight of the nucleic acid extraction device, and the length of the base 3 along the opening direction of the extraction cavity 101 is 120-180mm, such as Figure 2 As shown, the lateral distance between the right ends of the two horizontal sections 1031 is 20-40mm, and the lateral distance between the right end of the lower horizontal section 1031 and the right end of the first slide groove 102 is also 20-40mm, so as to avoid affecting the balance of the nucleic acid extraction device when the base 3 is pulled out.

[0075] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A fully automated nucleic acid extraction apparatus, characterized by: Including body (1), cabinet door (2), base (3) and guide mechanism (4), wherein, The body (1) is provided with a extraction cavity (101) on the side; The cabinet door (2) is detachably fixed on the body (1) and blocks the extraction cavity (101), the side wall in the extraction cavity (101) is provided with a first chute (102) and at least one second chute (103), the second chute (103) is connected with the first chute (102) at the end away from the cabinet door (2), and the end of the second chute (103) close to the cabinet door (2) is located above the end of the first chute (102) close to the cabinet door (2); The guide mechanism (4) includes a sliding seat (41), a connecting rod (42) and a wheel disc (43), the sliding seat (41) is slidingly arranged in the extraction cavity (101); the two ends of the connecting rod (42) are rotatably arranged on the sliding seat (41) and the base (3), respectively, and each group of the connecting rod (42) is provided with a plurality of connecting rods (42), and a plurality of the connecting rods (42) are arranged in parallel and at intervals; the wheel disc (43) is fixedly or rotatably arranged on the connecting rod (42) and is slidingly or rollingly connected with the first chute (102) and the second chute (103); The first chute (102) is arranged in the horizontal direction; the second chute (103) includes a horizontal section (1031) and a communication section (1032), a plurality of the horizontal sections (1031) and the first chute (102) are arranged in parallel and at intervals; the two ends of the communication section (1032) are respectively connected with the end of the horizontal section (1031) away from the cabinet door (2) and the first chute (102), the communication section (1032) is arc-shaped, and the centers of a plurality of the communication sections (1032) are the same, and the radii and arc lengths are different; The sliding resistance of the sliding seat (41) in the body (1) is greater than the moving resistance of the wheel disc (43) in the second chute (103) and the rotating resistance of the connecting rod (42); the second chute (103) is provided with two, the communication section (1032) away from the cabinet door (2) is connected with the end of the first chute (102) away from the cabinet door (2), and when the wheel disc (43) moves into the horizontal section (1031) above, the end of the connecting rod (42) close to the wheel disc (43) is located above the end of the connecting rod (42) away from the wheel disc (43), and when the wheel disc (43) moves into the horizontal section (1031) below or the first chute (102), the end of the connecting rod (42) close to the wheel disc (43) is located below the end of the connecting rod (42) away from the wheel disc (43). The base (3) comprises a supporting seat (31), a sliding frame (32) and a connecting bolt (33), wherein the supporting seat (31) is rotationally connected with the connecting rod (42); the sliding frame (32) is slidingly arranged on the bottom side of the supporting seat (31), and the sliding direction of the sliding frame (32) is parallel to the opening direction of the first sliding groove (102); the connecting bolt (33) is threadedly connected with the supporting seat (31) and can be clamped with the sliding frame (32); the stopper (5) is rotationally arranged on the machine body (1) and abuts and rolls with one end of the sliding frame (32) close to the connecting rod (42), and the cross section of the stopper (5) is non-circular. The distance between the horizontal section (1031) close to the cabinet door (2) and the cabinet door (2) gradually decreases from top to bottom, and the distance between the horizontal section (1031) close to the cabinet door (2) and the cabinet door (2) is greater than the distance between the first sliding groove (102) close to the cabinet door (2) and the cabinet door (2).

2. The fully automated nucleic acid extraction apparatus of claim 1, wherein: The wheel disc (43) comprises a fixed rod (431), a moving disc (432) and two rotating discs (433), wherein, Both ends of the fixed rod (431) are rotationally arranged on two connecting rods (42) in the same group, respectively; The moving disc (432) is fixedly arranged on the fixed rod (431) and slidingly arranged in the first sliding groove (102) and the second sliding groove (103); Both rotating discs (433) are rotationally arranged on the moving disc (432) and rollingly connected with the upper and lower sides in the first sliding groove (102) and the horizontal section (1031), respectively, and the two rotating discs (433) are rollingly connected, and the outer diameter of the moving disc (432) is less than 2 times the outer diameter of the rotating disc (433).

3. The fully automated nucleic acid extraction apparatus of claim 2, wherein: The wheel disc (43) further comprises a sliding rod (434) and a spring (435), wherein, The sliding rod (434) is slidingly arranged on the moving disc (432); The spring (435) is fixedly arranged on the moving disc (432), and one end of the spring (435) abuts one end of the sliding rod (434), so that the other end of the sliding rod (434) abuts one side of the communication section (1032) close to the cabinet door (2).

4. The fully automated nucleic acid extraction apparatus of claim 3, wherein: Two sliding rods (434) and two springs (435) are arranged on each moving disc (432), the sliding rod (434) and the spring (435) correspond to each other, and the two sliding rods (434) abut one side of the communication section (1032) close to the cabinet door (2) and one side of the communication section (1032) away from the cabinet door (2), respectively.

5. The fully automated nucleic acid extraction apparatus of claim 1, wherein: A mounting cavity (104) is formed on the machine body (1) away from the extraction cavity (101), the mounting cavity (104) is in communication with the extraction cavity (101), and is used for mounting a driving member of a pipetting module.

6. The fully automated nucleic acid extraction apparatus of claim 5, wherein: The position limiter (5) is provided with two, two position limiters (5) are respectively attached and rollingly connected at two ends of the sliding frame (32), and the cross section of the position limiter (5) is a Lely triangle.

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