Intelligent microbiological detection pretreatment workstation
By designing an intelligent microbial detection pre-processing workstation and using modular components to automate the detection steps, the inefficiency and safety hazards caused by repetitive operations in the existing technology are solved, and an efficient and automated microbial detection pre-processing process is achieved.
Patent Information
- Application Number
- CN202411950321.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-27
- Publication Date
- 2025-03-28
AI Technical Summary
There are a lot of repetitive operations in existing microbial detection technologies, which leads to boring work and inefficient efficiency, and requires multiple people to cooperate, which poses safety risks.
An intelligent microbial detection pre-processing workstation is designed, including multiple modular components such as a Petri dish turntable module, a Petri dish transfer module, a homogeneous hit module, a vortex oscillation module, a rack storage module, a pipetting robot, a clamping robot and a culture medium feed module. Through the coordinated work of these modules, the steps from sample to culture are automatically completed.
The pre-processing of microbial detection is achieved at a high speed and efficiency, which improves the laboratory's production capacity, reduces labor costs, and only one staff member can complete the work, and the modular design can adjust the steps according to the detection situation.
Smart Images

Figure CN119709388A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of microbial detection, in particular to an intelligent microbial detection pre-processing workstation. Background Art
[0002] Microbiological testing of food and cosmetics is one of the important indicators to measure the hygienic quality of food and cosmetics, and is also one of the scientific bases for determining whether the inspected food is edible. Through microbiological testing of food and cosmetics, we can judge the food and cosmetics processing environment and food hygiene, make a correct evaluation of the degree of bacterial contamination of food and cosmetics, and provide a scientific basis for various health management work.
[0003] For food and cosmetics testing laboratories, there are a large number of samples every day, and there are many food and cosmetics microbial testing items. The process is repetitive, and there are a lot of mechanical and repetitive operations. The work is boring and tedious, and people cannot work with maximum creativity. Microbial automation solutions can replace a lot of repetitive work in the experimental process, reduce laboratory labor expenses, and ensure the stability of experimental results.
[0004] In the manual operation environment of microbial detection tests, one process requires the cooperation of at least four to five people. Not only is the workload heavy, but it also poses a great threat to the safety of the staff. Summary of the invention
[0005] The main technical problem solved by the present invention is to provide an intelligent microbial detection pretreatment workstation, which can complete the steps from sample to culture at high speed and efficiency, and the modular combination can adjust the steps according to the actual detection situation.
[0006] In order to solve the above technical problems, a technical solution adopted by the present invention is:
[0007] An intelligent microbial detection pre-processing workstation includes a culture dish turntable module, a culture dish transmission module, a homogenization slapping module, a vortex oscillation module, a material rack storage module, a liquid transfer robot, a gripping robot and a culture medium supply module;
[0008] The culture dish turntable module comprises culture dish positioning grooves circumferentially distributed on the turntable, and the circumferential side of the turntable is provided with an upper dish station, a culture medium injection station, an inoculation / coating station and a lower dish station in a clockwise manner; the upper dish station and the lower dish station are respectively provided with a transplanting electric module; the culture medium injection station is provided with an injection unit, and the injection unit is used to inject culture solution into the culture dish at the culture medium injection station, and the injection unit can inject at least three different culture solutions; the inoculation / coating station is provided with a shaking module, and the shaking module is used to shake the culture dish at the inoculation station;
[0009] The turntable can rotate in a specific direction, and there are 6 groups of culture dish positioning grooves, which are culture dish cover positioning grooves and dish box positioning grooves arranged radially inward and outward of the turntable; a code reader is also arranged on the circumference of the turntable, and the code reader is used to read the code on the bottom of the flat dish at the dish loading station;
[0010] The culture dish transmission module includes a dish loading unit, a dish storage unit, and a dish discharging unit. The dish loading unit transfers the culture dish to the upper dish station, the dish storage unit is used to receive and store the culture dish at the lower dish station, and the dish discharging unit is used to transfer the culture dish in the dish storage unit to the outside. The dish loading unit is connected to an external dish feeding unit, the dish storage unit is a graduated storage rack, and the dish discharging unit is connected to an external receiving unit.
[0011] The rack storage module is used to store pipette tips, test tubes and homogenizing bags;
[0012] The homogenizing and slapping module is used to quantitatively dilute the sample in the homogenizing bag and then homogenize it;
[0013] The vortex oscillation module is used to quantitatively dilute and homogenize the sample in the test tube:
[0014] The gripping robot is used to: grip the homogenizing bag of the rack storage module and send it to the homogenizing slapping module, grip the test tube of the rack storage module and send it to the vortex oscillation module, and remove the plug of the test tube;
[0015] The liquid transfer robot is used to absorb the quantitatively diluted sample and add it to the culture dish of the culture dish turntable module;
[0016] The culture medium supply module is used to transport the culture solution to the injection unit. The culture medium supply module includes a plurality of peristaltic pumps, one end of each peristaltic pump is connected to a culture solution unit, and the other end is connected to the injection unit. The culture solution unit stores large intestine culture solution, mold / yeast psychrophilic bacteria culture solution, and total colony count culture solution.
[0017] Furthermore, the transplanting electric module includes an electric rotating clamp driven by a two-axis transplanting unit for translation and lifting. The contact surface between the clamping hand of the electric rotating clamp and the flat plate is coated with Teflon so as not to damage the flat plate. The transplanting electric module can realize the actions of clamping and transferring the flat plate, as well as opening and installing the cover.
[0018] Furthermore, the workstation further comprises a workbench, the culture dish turntable module is installed on the workbench, the culture dish transfer module is arranged below the workbench, and the workbench is respectively provided with an empty dish discharge port and a flat dish inlet port corresponding to the dish loading station and the dish loading station;
[0019] A plate lifting module is provided between the plate loading unit and the empty plate discharging port, and a plate lifting module is provided between the plate storage unit and the plate storage port;
[0020] The flat plate lifting module includes a lifting unit, a telescopic electric cylinder and a flat plate pallet. The flat plate pallet is used to lift and lower the flat plate. The flat plate is arranged at the output end of the telescopic electric cylinder. The output end of the telescopic electric cylinder drives the flat plate pallet to translate. The lifting electric cylinder drives the telescopic electric cylinder to lift and lower the flat plate pallet.
[0021] Furthermore, the homogenizing and slapping module comprises a working space, wherein a feeding port and a discharging port are respectively arranged at the upper and lower ends of the working space, and a bag opening module, a sealing module and a slapping module are arranged in sequence from top to bottom along the working space, wherein the bag opening module is used to open the bag opening of the homogenizing bag, the sealing module is used to seal the homogenizing bag, and the slapping module is used to slap the homogenizing bag;
[0022] The homogenizing and slapping module also includes a first diluent injection unit, which is used to add a predetermined amount of diluent into the homogenizing bag; a homogenizing bag collecting box connected to the feeding port is arranged below the homogenizing and slapping module; the homogenizing bag falls into the homogenizing bag collecting box through a slide.
[0023] Furthermore, the vortex oscillation module includes a first electric clamp unit, a mixing oscillator and a second diluent injection unit; a test tube placement slot is provided at the upper end of the mixing oscillator, and the first electric clamp unit can limit the test tube to be located in the test tube placement slot; the second diluent injection unit is used to add a predetermined amount of diluent into the test tube.
[0024] The first diluent injection unit and the second diluent injection unit are both connected to the diluent supply unit.
[0025] The diluent supply unit comprises a peristaltic pump, one end of the peristaltic pump is connected to the diluent storage unit, and the other end is connected to the first diluent injection unit / the second diluent injection unit.
[0026] Furthermore, the gripping robot includes a multi-axis robot, a control end of the multi-axis robot is equipped with a second electric gripper unit, a control end of the second electric gripper unit is equipped with a clamping unit, the clamping unit includes relatively arranged clamping members, the second electric gripper unit can drive the two clamping members to approach or move away from each other, the side surface on which the two clamping members are relatively close is a clamping surface, and the clamping surface includes a vertical plane suitable for clamping a bag-like body and a curved surface suitable for clamping a columnar body.
[0027] Furthermore, the shaking module includes a third electric clamp unit, a shaking robot and an upper and lower electric module. The third electric clamp unit is installed at the control end of the shaking robot, and the upper and lower electric modules drive the shaking robot to move up and down.
[0028] Specifically: the electric gripper clamps the dish box, the upper and lower electric modules drive the up and down movement, and the shaking motor of the shaking robot drives the swing mechanism to shake the dish box back and forth and left and right to meet the process requirements.
[0029] Furthermore, the rack storage module includes a pipette tip rack, a test tube rack and a homogenizing bag rack, and the pipette tip rack, the test tube rack and the homogenizing bag rack all include a quick-change rack structure; the quick-change rack structure includes a rack body and an installation groove, and the bottom of the rack body can slide along the installation groove. The rack body includes a placement structure for placing pipette tips / test tubes / homogenizing bags in an array.
[0030] Furthermore, the workstation also includes a quick-change module, which includes a module bracket, and a pipette pump quick-change module and a coating head quick-change module are placed on the upper end of the module bracket; according to the inoculation / coating station setting, the pipette pump quick-change module / the coating head quick-change module is selected to be detachably installed on the control end of the pipetting robot; the pipette pump quick-change module can load and unload the pipette tip; the coating head quick-change module can load and unload the coating head.
[0031] Furthermore, the workstation also includes a coating head silo and a recycling box, wherein the recycling box is used to receive discarded pipette tips and coating heads of the pipetting robot; the coating head silo includes a coating head lifting servo slide.
[0032] The beneficial technical effects of the present invention are:
[0033] The workflow of the microbial detection pretreatment workstation of the present invention is designed in accordance with the requirements of the national food safety standard GB4789 for food microbiology inspection, and can complete the steps from sample to culture at high speed and efficiency, effectively increasing the production capacity by more than three times and reducing labor costs. The workstation only needs to be equipped with one staff member; the modular combination can adjust the steps according to the actual detection situation. BRIEF DESCRIPTION OF THE DRAWINGS
[0034] Figure 1 It is a schematic diagram of the overall structure of the present invention (stereoscopic view);
[0035] Figure 2 It is a schematic diagram of the overall structure of the present invention (top view);
[0036] Figure 3 It is a structural schematic diagram of the culture dish turntable module of the present invention;
[0037] Figure 4 It is a structural schematic diagram of the bottom of the workbench of the present invention (culture dish transmission module);
[0038] Figure 5 It is a structural schematic diagram of the shaking module of the present invention;
[0039] Figure 6 It is a structural schematic diagram of the electric module for transplanting plants according to the present invention;
[0040] Figure 7 It is a structural schematic diagram of the homogenizing slapping module of the present invention (including the outer box);
[0041] Figure 8 It is a schematic diagram of the internal structure of the homogenous slapping module of the present invention;
[0042] Fig. 9 It is a structural schematic diagram of the vortex oscillation module of the present invention;
[0043] Fig.10 It is a structural schematic diagram of the plate lifting module of the present invention;
[0044] Fig.11 It is a structural schematic diagram of the gripping robot of the present invention;
[0045] Fig.12 It is a schematic structural diagram of the clamping member of the present invention;
[0046] Fig.13 It is a structural schematic diagram of the quick-change rack structure of the present invention;
[0047] Fig.14 It is a structural schematic diagram of the quick-change module of the present invention;
[0048] Fig.15 It is a structural schematic diagram of the coating head silo of the present invention;
[0049] The parts in the accompanying drawings are marked as follows:
[0050] 1. Culture dish turntable module; 11. Turntable; 12. Culture dish positioning slot; 13. Liquid injection unit; 14. Shaking module; 141. Third electric gripper unit; 142. Shaking manipulator; 143. Up and down electric module; 15. Transplantation electric module; 151. Two-axis transplantation unit; 152. Electric rotating gripper; 16. Code reader;
[0051] 2. Culture dish transmission module; 21. Plate loading unit; 22. Plate storage unit; 23. Plate discharging unit; 24. Plate lifting module; 241. Lifting unit; 242. Telescopic electric cylinder; 243. Plate support plate;
[0052] 3. Homogenizing and slapping module; 31. Working space; 32. Feeding port; 33. Discharging port; 34. Bag opening module; 35. Sealing module; 36. Slapping module; 37. First diluent injection unit; 38. Homogenizing bag collecting box; 39. Slideway;
[0053] 4. Vortex oscillation module; 41. First electric gripper unit; 42. Mixing oscillator; 421. Test tube placement slot; 43. Second diluent injection unit;
[0054] 5. Rack storage module; 51. Pipette tip rack; 52. Test tube rack; 53. Homogenizing bag rack; 54. Quick-change rack structure; 541. Rack body; 542. Installation slot;
[0055] 6. Liquid transfer robot; 61. Quick change module; 62. Module bracket; 63. Liquid transfer pump quick change module; 64. Coating head quick change module;
[0056] 7. Gripping robot; 71. Multi-axis robot; 72. Second electric gripper unit; 73. Clamping unit; 74. Clamping member; 741. Gripping surface; 742. Vertical plane; 743. Arc surface;
[0057] 8. Peristaltic pump;
[0058] 9. Workbench; 91. Empty dish outlet; 92. Flat dish inlet; 93. Coating head silo; 94. Recycling box. DETAILED DESCRIPTION
[0059] In order to more clearly understand the technical means of the present invention and implement it according to the contents of the specification, the specific implementation methods of the present invention are further described in detail below in conjunction with the drawings and examples. The following examples are used to illustrate the present invention but are not used to limit the scope of the present invention.
[0060] This specific embodiment discloses in detail an intelligent microbial detection pre-processing workstation, such as Figures 1 to 15 As shown, it includes a culture dish turntable module 1, a culture dish transmission module 2, a homogenization slapping module 3, a vortex oscillation module 4, a material rack storage module 5, a pipetting robot 6, a gripping robot 7 and a culture medium supply module;
[0061] The culture dish turntable module includes a culture dish positioning groove 12 circumferentially distributed on the turntable 11, and the circumferential side of the turntable is sequentially provided with an upper dish station, a culture medium injection station, an inoculation / coating station and a lower dish station in a clockwise manner; the upper dish station and the lower dish station are respectively provided with a transplanting electric module 15; the culture medium injection station is provided with an injection unit 13, and the injection unit is used to inject culture fluid into the culture dish at the culture medium injection station, and the injection unit can inject at least three different culture fluids; the inoculation / coating station is provided with a shaking module 14, and the shaking module is used to shake the culture dish at the inoculation station;
[0062] The turntable can rotate in a specific direction, and there are 6 groups of culture dish positioning grooves, which are culture dish cover positioning grooves and dish box positioning grooves arranged radially inward and outward of the turntable; a code reader 16 is also arranged on the peripheral side of the turntable, and the code reader is used to read the code on the bottom of the flat dish at the dish loading station;
[0063] The culture dish transmission module includes a culture dish loading unit 21, a culture dish storage unit 22 and a culture dish discharging unit 23. The culture dish loading unit transmits the culture dish to the loading dish station, the culture dish storage unit is used to receive and store the culture dishes at the lower dish station, and the culture dish discharging unit is used to transfer the culture dishes in the culture dish storage unit to the outside; wherein, the culture dish loading unit is connected to the external culture dish feeding unit, the culture dish storage unit is a graduated storage rack; and the culture dish discharging unit is connected to the external receiving unit.
[0064] The rack storage module is used to store pipette tips, test tubes and homogenizing bags;
[0065] The homogenizing and tapping module is used to quantitatively dilute the sample in the homogenizing bag and then homogenize it;
[0066] The vortex oscillation module is used to quantitatively dilute and homogenize the samples in the test tube:
[0067] The gripping robot is used to: grip the homogenizing bag of the material rack storage module and send it to the homogenizing slapping module, grip the test tube of the material rack storage module and send it to the vortex oscillation module, and remove the plug of the test tube;
[0068] The liquid transfer robot is used to absorb the quantitatively diluted sample and add it to the culture dish of the culture dish turntable module;
[0069] The culture medium supply module is used to deliver culture solution to the injection unit. The culture medium supply module includes a plurality of peristaltic pumps 8, one end of each peristaltic pump is connected to a culture solution unit, and the other end is connected to an injection unit. The culture solution unit stores large intestine culture solution, mold / yeast psychrophilic bacteria culture solution, and total colony count culture solution.
[0070] Preferably, the transplanting electric module includes an electric rotating clamp 152 driven by a two-axis transplanting unit 151 for translation and lifting. The contact surface between the clamping hand of the electric rotating clamp and the flat plate is coated with Teflon so as not to damage the flat plate. The transplanting electric module can realize the actions of clamping and transferring the flat plate, as well as opening and installing the cover.
[0071] Preferably, the workstation further comprises a workbench 9, the culture dish turntable module is installed on the workbench, the culture dish transfer module is arranged below the workbench, and the workbench is respectively provided with an empty dish discharge port 91 and a flat dish inlet port 92 corresponding to the dish loading station and the dish loading station;
[0072] A plate lifting module 24 is provided between the plate loading unit and the empty plate discharge port, and a plate lifting module is provided between the plate storage unit and the plate storage port;
[0073] The plate lifting module includes a lifting unit 241, a telescopic electric cylinder 242 and a plate tray 243. The plate tray is used to lift the plate. The plate is set at the output end of the telescopic electric cylinder. The output end of the telescopic electric cylinder drives the plate tray to translate. The lifting electric cylinder drives the telescopic electric cylinder to lift and lower the plate tray.
[0074] Preferably, the homogenizing and slapping module comprises a working space 31, wherein a feeding port 32 and a discharging port 33 are respectively arranged at the upper and lower ends of the working space, and a bag opening module 34, a sealing module 35 and a slapping module 36 are sequentially arranged along the working space from top to bottom, wherein the bag opening module is used to open the bag opening of the homogenizing bag, the sealing module is used to seal the homogenizing bag, and the slapping module is used to slap the homogenizing bag;
[0075] The homogenizing and slapping module also includes a first diluent injection unit 37, which is used to add a predetermined amount of diluent into the homogenizing bag; a homogenizing bag collecting box 38 connected to the discharge port is arranged below the homogenizing and slapping module; the homogenizing bag falls into the homogenizing bag collecting box through a slide 39.
[0076] Preferably, the vortex oscillation module includes a first electric clamp unit 41, a mixing oscillator 42 and a second diluent injection unit 43; a test tube placement slot 421 is provided at the upper end of the mixing oscillator, and the first electric clamp unit can limit the test tube to be located in the test tube placement slot; the second diluent injection unit is used to add a predetermined amount of diluent into the test tube.
[0077] The first diluent injection unit and the second diluent injection unit are both connected to the diluent supply unit.
[0078] The diluent supply unit comprises a peristaltic pump, one end of the peristaltic pump is connected to the diluent storage unit, and the other end is connected to the first diluent injection unit / the second diluent injection unit.
[0079] Preferably, the clamping robot includes a multi-axis robot 71, a second electric clamping unit 72 is installed at the control end of the multi-axis robot, a clamping unit 73 is installed at the control end of the second electric clamping unit, the clamping unit includes relatively arranged clamping members 74, the second electric clamping unit can drive the two clamping members to approach or move away from each other, the side surface on which the two clamping members are relatively close is a clamping surface 741, and the clamping surface includes a vertical plane 742 suitable for clamping a bag-like body and an arc surface 743 suitable for clamping a columnar body.
[0080] Preferably, the shaking module includes a third electric clamp unit 141, a shaking manipulator 142 and an upper and lower electric module 143. The third electric clamp unit is installed at the control end of the shaking manipulator, and the upper and lower electric modules drive the shaking manipulator to move up and down. The electric clamp clamps the dish box, and the upper and lower electric modules drive the upper and lower electric modules to move up and down. The shaking motor of the shaking manipulator drives the swing mechanism to realize the shaking of the dish box back and forth and left and right to meet the process requirements.
[0081] Preferably, the rack storage module includes a pipette tip rack 51, a test tube rack 52 and a homogenizing bag rack 53, and the pipette tip rack, the test tube rack and the homogenizing bag rack all include a quick-change rack structure 54; the quick-change rack structure includes a rack body 541 and a mounting groove 542, and the bottom of the rack body can slide along the mounting groove. The rack body includes a placement structure for placing pipette tips / test tubes / homogenizing bags in an array.
[0082] Preferably, the workstation further comprises a quick-change module 61, which comprises a module bracket 611, and a pipette pump quick-change module 612 and a coating head quick-change module 613 are placed on the upper end of the module bracket; according to the inoculation / coating station setting, the pipette pump quick-change module / coating head quick-change module is selected to be detachably installed on the control end of the pipette robot;
[0083] The pipette pump quick-change module can load and unload pipette tips;
[0084] The coating head quick change module can load and remove the coating head.
[0085] The collaborative robot is combined with a quick-change module to achieve pouring inoculation and coating inoculation.
[0086] Preferably, the workstation further comprises a coating head silo 93 and a recycling box 94, wherein the recycling box is used to receive discarded pipette tips and coating heads of the pipetting robot; the coating head silo comprises a coating head lifting servo slide.
[0087] The workflow of the microbial detection pretreatment workstation of the present invention is designed according to the requirements of the national food safety standard GB4789 food microbiology inspection, and can complete the steps from sample to culture at high speed and high efficiency; the modular combination can adjust the steps according to the actual detection situation.
[0088] The workflow is as follows:
[0089] The gripping robot grabs the homogenizing bag to the homogenizing slapping module. The homogenizing bag contains 25 ml of stock solution. The homogenizing slapping module has a code scanning mechanism to detect whether the homogenizing bag is in place and scan the code to confirm the sample process. The bag opening module of the homogenizing slapping module opens the homogenizing bag, and the first diluent injection sheet injects 225 ml of diluent. The bag opening module clamps the homogenizing bag, and the slapping module slaps the homogenizing bag. The bag opening module releases the homogenizing bag, and the pipetting robot absorbs 9 ml of the 10-fold diluted sample in the homogenizing bag. The sealing module seals the homogenizing bag, and the processed homogenizing bag falls into the recycling box.
[0090] The gripping robot grips the test tube and places it in the vortex oscillation module (test tube placement slot of the mixing oscillator). The first electric gripper unit clamps the test tube, the gripping robot pulls out the test tube plug, the pipetting robot injects 1 ml of the 10-fold diluted sample into the test tube, and then adds it to the culture dish on the turntable. The gripping robot replaces the pipette tip, and the second diluent injection unit injects 9 ml of diluent into the test tube, oscillates and mixes to obtain a 100-fold diluted sample. The pipetting robot absorbs the 100-fold diluted sample and adds it to the culture dish on the turntable. The gripping robot places the test tube in the test tube rack;
[0091] The above steps complete two dilution gradients, which are suitable for detecting Escherichia coli, molds, yeasts and psychrophilic bacteria. If the total number of colonies needs to be detected, three dilution gradients are required. The specific steps are as follows:
[0092] The gripping robot grips another test tube to the vortex oscillation module, and the first electric gripper unit clamps the test tube. The gripping robot removes the test tube plug, and the pipette robot adds the 100-fold diluted sample to the test tube, and then replaces the pipette tip. The second diluent injection unit injects 9 ml of diluent into the test tube, oscillates and mixes, and prepares a 1000-fold diluted sample. The pipette robot absorbs the 1000-fold diluted sample and adds it to the culture dish on the turntable, and the gripping robot places the test tube in the test tube rack.
[0093] In the description of the present invention, it should be noted that the directions or positional relationships indicated by terms such as "center", "up", "down", "left", "right", "vertical", "horizontal", "front" and "back" are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on the present invention.
[0094] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "set" should be understood in a broad sense, for example, they can be fixedly connected or set, or detachably connected or set, or integrally connected or set. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.
[0095] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. It should be pointed out that a person skilled in the art can make several improvements and modifications without departing from the technical principles of the present invention, and these improvements and modifications should also be regarded as within the scope of protection of the present invention.
Claims
1. Intelligent microbial detection pre-treatment workstation, characterized by: It comprises a culture dish turntable module (1), a culture dish transmission module (2), a homogenization slapping module (3), a vortex oscillation module (4), a material rack storage module (5), a liquid transfer robot (6), a gripping robot (7) and a culture medium supply module; The culture dish turntable module comprises culture dish positioning grooves (12) circumferentially distributed on the turntable (11); the turntable is provided with an upper dish station, a culture medium injection station, an inoculation / coating station and a lower dish station in a clockwise order; the upper dish station and the lower dish station are respectively provided with a transplanting electric module (15); the culture medium injection station is provided with an injection unit (13), the injection unit is used to inject culture solution into the culture dish at the culture medium injection station, and the injection unit can inject at least three different culture solutions; the inoculation / coating station is provided with a shaking module (14), the shaking module is used to shake the culture dish at the inoculation station; The culture dish transmission module comprises a culture dish loading unit (21), a culture dish storage unit (22) and a culture dish discharging unit (23), wherein the culture dish loading unit transfers the culture dish to the upper dish station, the culture dish storage unit is used to receive and store the culture dish at the lower dish station, and the culture dish discharging unit is used to transfer the culture dish in the culture dish storage unit to the outside; The rack storage module is used to store pipette tips, test tubes and homogenizing bags; The homogenizing and slapping module is used to quantitatively dilute the sample in the homogenizing bag and then homogenize it; The vortex oscillation module is used to quantitatively dilute and homogenize the sample in the test tube: The gripping robot is used to: grip the homogenizing bag of the rack storage module and send it to the homogenizing slapping module, grip the test tube of the rack storage module and send it to the vortex oscillation module, and remove the plug of the test tube; The liquid transfer robot is used to absorb the quantitatively diluted sample and add it to the culture dish of the culture dish turntable module; The culture medium supply module is used to transport the culture solution to the injection unit.
2. The intelligent microbial detection pre-processing workstation according to claim 1 is characterized by: The electric transplanting module comprises an electric rotating clamp (152) driven by a two-axis transplanting unit (151) to perform translation and lifting, and the contact surface between the clamping hand of the electric rotating clamp and the plate is coated with Teflon.
3. The intelligent microbial detection pre-processing workstation according to claim 1 is characterized by: The workstation further comprises a workbench (9), the culture dish turntable module is mounted on the workbench, the culture dish transfer module is arranged below the workbench, and the workbench is respectively provided with an empty dish discharge port (91) and a culture dish inlet port (92) corresponding to the dish loading station and the dish loading station; A plate lifting module (24) is provided between the plate loading unit and the empty plate discharge port, and a plate lifting module is provided between the plate storage unit and the plate storage port; The plate lifting module comprises a lifting unit (241), a telescopic electric cylinder (242) and a plate support plate (243), wherein the plate support plate is used to lift and lower the plate, the plate is arranged at the output end of the telescopic electric cylinder, the output end of the telescopic electric cylinder drives the plate support plate to translate, and the lifting electric cylinder drives the telescopic electric cylinder to lift and lower the plate to achieve lifting and lowering of the plate support plate.
4. The intelligent microbial detection pre-processing workstation according to claim 1 is characterized in that: The homogenizing and slapping module comprises a working space (31), wherein the upper and lower ends of the working space are respectively provided with a feeding port (32) and a discharging port (33), and a bag opening module (34), a sealing module (35) and a slapping module (36) are sequentially arranged along the working space from top to bottom, wherein the bag opening module is used to open the bag opening of the homogenizing bag, the sealing module is used to seal the homogenizing bag, and the slapping module is used to perform a slapping action on the homogenizing bag; The homogenizing and beating module also includes a first diluent injection unit (37), which is used to add a predetermined amount of diluent into the homogenizing bag; a homogenizing bag collecting box (38) connected to the feeding port is arranged below the homogenizing and beating module.
5. The intelligent microbial detection pre-processing workstation according to claim 1 is characterized by: The vortex oscillation module comprises a first electric clamp unit (41), a mixing oscillator (42) and a second diluent injection unit (43); a test tube placement slot (421) is provided at the upper end of the mixing oscillator, and the first electric clamp unit can limit the test tube to be located in the test tube placement slot; the second diluent injection unit is used to add a predetermined amount of diluent into the test tube.
6. The intelligent microbial detection pre-processing workstation according to claim 1 is characterized by: The gripping robot comprises a multi-axis robot (71), a control end of the multi-axis robot is equipped with a second electric gripper unit (72), a control end of the second electric gripper unit is equipped with a clamping unit (73), the clamping unit comprises clamping members (74) arranged opposite to each other, the second electric gripper unit can drive two clamping members to approach or move away from each other, a side surface of the two clamping members being relatively close to each other is a gripping surface (741), and the gripping surface comprises a vertical plane (742) suitable for gripping a bag-like body and an arcuate surface (743) suitable for gripping a columnar body.
7. The intelligent microbial detection pre-processing workstation according to claim 1 is characterized by: The shaking module comprises a third electric clamp unit (141), a shaking manipulator (142) and an upper and lower electric module (143), wherein the third electric clamp unit is installed at the control end of the shaking manipulator, and the upper and lower electric module drives the shaking manipulator to move up and down.
8. The intelligent microbial detection pre-processing workstation according to claim 1 is characterized by: The material rack storage module comprises a pipette tip material rack (51), a test tube material rack (52) and a homogenizing bag material rack (53), and the pipette tip material rack, the test tube material rack and the homogenizing bag material rack all comprise a quick-change rack structure (54); The quick-change rack structure comprises a rack body (541) and an installation groove (542), and the bottom of the rack body can slide along the installation groove.
9. The intelligent microbial detection pre-processing workstation according to claim 1 is characterized by: The workstation further comprises a quick-change module (61), the quick-change module comprising a module bracket (611), a liquid transfer pump quick-change module (612) and a coating head quick-change module (613) being placed on the upper end of the module bracket; According to the inoculation / coating station setting, the pipetting pump quick-change module / the coating head quick-change module is selected to be detachably installed on the control end of the pipetting robot; The pipette pump quick-change module can load and unload pipette tips; The coating head quick-change module can load and unload the coating head.
10. The intelligent microbial detection pre-processing workstation according to claim 9 is characterized in that: The workstation also includes a coating head silo (93) and a recycling box (94), wherein the recycling box is used to receive discarded pipette tips and coating heads of the pipetting robot; the coating head silo includes a coating head lifting servo slide.
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