Integrated esophageal cancer tumor cell culture and separation system

Abstract

The invention relates to the technical field of cell culture, and particularly discloses an integrated esophageal cancer tumor cell culture and separation system which comprises a box body and an auxiliary assembly, the auxiliary assembly comprises a fixing frame arranged in the box body, a side plate is slidably clamped on one side of the box body, the fixing frame is connected to the side, close to the box body, of the side plate, and a plurality of clamping grooves are formed in the fixing frame; a culture bottle is clamped in each clamping groove, a sealing cover is rotationally clamped at one end of each culture bottle, a limiting groove is formed in the box body, a sliding plate slides in the limiting groove, and a clamping assembly is arranged on the sliding plate; an auxiliary assembly arranged in the device can assist an experimenter in adding a culture medium, digestive juice and other experimental solutions into the culture bottle when the cell density in the culture bottle reaches the passage critical density, when the device is used for cell culture, after tumor cells are inoculated into the culture bottle, the culture bottle is clamped into a clamping groove formed in a fixing frame, and the culture bottle is fixed through the clamping groove. The growth condition of the tumor cells in the box body can be observed through the observation window by translating the sliding plate arranged in the limiting groove.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and more specifically, to an integrated esophageal cancer tumor cell culture and separation system. Background Art

[0002] Cell culture refers to a method of simulating the human body's internal environment in vitro to enable cells to survive, grow, reproduce, and maintain their main structures and functions. Cell culture is also called cell cloning technology, and its formal term in biology is cell culture technology. In vitro culture of cancer cells has become the main application material for human research and prevention of cancer. For different cancers, it is necessary to culture and conduct experiments on the corresponding cells of the cancer.

[0003] However, most existing esophageal cancer tumor cell culture and separation systems require manual control of the separation process during cell passaging, and require repeated addition of culture medium, digestive fluid and other liquids to the culture bottle. The operation is relatively cumbersome and requires a long time for separation operation, thereby reducing the effectiveness of the device. Summary of the invention

[0004] The present invention provides an integrated esophageal cancer tumor cell culture and separation system to solve the problem that most esophageal cancer tumor cell culture and separation systems in the prior art require manual control of the separation process during cell passage, which is cumbersome to operate and takes a long time to perform the separation operation.

[0005] In order to achieve the above object, the present invention provides the following technical solutions: An integrated esophageal cancer tumor cell culture and separation system comprises a box body and an auxiliary component, wherein the auxiliary component comprises a fixed frame arranged in the box body, a side panel is slidably clamped on one side of the box body, the side panel is connected to a fixed frame close to a side of the box body, a plurality of slots are provided in the fixed frame, each of the slots is clamped with a culture bottle, one end of the culture bottle is rotatably clamped with a sealing cover, a limiting groove is provided in the box body, a sliding plate slides in the limiting groove, and a clamping component is provided on the sliding plate.

[0006] Preferably, a connecting rod is rotatably connected to the sliding plate, a connecting shaft is provided on one side of the connecting rod, a coil spring is provided at the hinge between the connecting rod and the sliding plate, a motor is fixedly connected to the connecting rod, and a connecting frame is rotatably connected to the motor output rod.

[0007] Preferably, the clamping assembly includes a pair of clips slidably mounted on the connecting frame, the two clips are arranged opposite to each other, the two clips are connected by a first spring, chamfers are arranged on opposite sides of the two clips, and the clips are clamped on the outside of the sealing cover.

[0008] Preferably, a control component is provided on the sliding plate, and the control component includes a longitudinal rod provided on the sliding plate, a connecting plate is passed through the longitudinal rod, the bottom end of the connecting plate is connected to the top wall of the sliding plate through a third spring, the top end of the connecting plate is connected to a sliding frame, and the sliding frame is clamped on the outside of the connecting shaft.

[0009] Preferably, the top of the sliding frame is connected to a button through a pressure rod, the top of the sliding plate is connected to a movable frame, the movable frame slides in a sliding groove opened at the top of the box body, one end of the movable frame is connected to a microscope, the top of the box body is provided with an observation window, the setting direction of the observation window is the same as the extension direction of the limiting groove, and the width of the pressure rod is slightly narrower than that of the movable frame.

[0010] Preferably, a plurality of pressing buttons are slidingly provided in the limiting groove, and the inner bottom wall of the pressing button is connected to the inner bottom wall of the box body through a second spring, the number of the pressing buttons is equal to the number of the slots, and the pressing button is connected to a connecting frame through one end, and the top of the connecting frame is rotatably connected to a support plate, a pair of side grooves are opened on the support plate, and the support plate is clamped on the bottom wall of the box body through the side grooves.

[0011] Preferably, a groove is provided at the bottom end of the inner wall of each slot, a support rod is rotatably provided on the bottom wall of the slot, the upper surface of the support rod is flush with the bottom wall of the slot, the bottom end of the support rod is connected to a bottom rod, and the bottom rod and the support plate are arranged in the same vertical plane.

[0012] Preferably, a collecting box is provided on the bottom wall of the box body, the opening of the collecting box is aligned with one end of the opening of the culture bottle, and a plurality of nozzles are provided on the top of the collecting box, and the nozzles are aligned with the culture bottle.

[0013] Preferably, a telescopic rod is provided on one side of the box body, and the top end of the output rod of the telescopic rod contacts the bottom surface of the side plate.

[0014] Preferably, a partition is provided in the box body, and a refrigeration compartment is provided on one side of the partition.

[0015] The principle and beneficial effects of this technical solution: (1) The auxiliary components provided in the present invention can assist the experimenter to add culture medium, digestion fluid and other experimental fluids into the culture bottle when the cell density in the culture bottle reaches the critical density for passage, thereby improving the separation efficiency. When using the device for cell culture, after the tumor cells are inoculated into the culture bottle, the culture bottle is inserted into the card slot provided in the fixed frame, and the insulation-related components in the box are started to culture the tumor cells in the incubator at a constant temperature. The sliding plate provided in the translation limit groove is translated. Since one end of the moving frame connected to the top of the sliding plate is connected to a microscope, and an observation window is provided at the top of the box, and the setting direction of the observation window is the same as the extension direction of the limit groove, the growth of the tumor cells in the box can be observed through the observation window during the translation of the sliding plate.

[0016] (2) The push button provided in the present invention can help the experimenter to pour the liquid in the culture bottle. When it is necessary to pour the liquid, the telescopic rod is driven to shorten, and the side plate contacted by the top end of the output rod end of the telescopic rod will drive the fixed frame to move downward. At this time, the support rod rotatably mounted on the bottom wall of the slot will move downward synchronously with the bottom rod. When the sliding plate moves to one side of the culture bottle, it will simultaneously press down the push button mounted in the limit groove on one side of the sliding plate. The push button will compress the second spring and drive the connecting frame connected to the push button to move downward. The bottom rod rotatably connected to the connecting frame will deflect upward on the bottom wall of the box body. When the support rod and the bottom rod move downward synchronously, the bottom rod will drive the support rod to deflect upward due to the compression with the support plate, so that the culture bottle mounted in the slot will deflect toward one end of the collection box. The culture bottle will pour out the supernatant in it for subsequent culture transfer. When liquid needs to be added, the telescopic rod is reset and the nozzle is opened to pump liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a schematic diagram of the structure after the present invention is split; Figure 3 for Figure 2 Schematic diagram of the enlarged structure of the A area in the middle; Figure 4 It is a schematic diagram of the overall structure of the present invention; Figure 5 for Figure 4 Schematic diagram of the enlarged structure of the middle B area; The figure marks in the drawings of the specification include: 1, box body; 2, collection box; 3, sealing cover; 4, fixing frame; 5, slide groove; 6, observation window; 7, telescopic rod; 8, motor; 9, connecting frame; 10, first spring; 11, clamp; 12, chamfer; 13, culture bottle; 14, connecting rod; 15, second spring; 16, moving frame; 17, button; 18, pressure rod; 19, connecting shaft; 20, slide frame; 21, connecting plate; 22, third spring; 23, longitudinal rod; 24, sliding plate; 25, partition; 26, side plate; 27, cold storage compartment; 28, slot; 29, groove; 30, support rod; 31, bottom rod; 32, support plate; 33, side groove; 34, connecting frame; 35, pressure button; 36, limit groove. DETAILED DESCRIPTION

[0018] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments: Embodiment:

[0019] like Figures 1 to 5 As shown, the present invention provides an integrated esophageal cancer tumor cell culture and separation system, including a box body 1 and an auxiliary component, the auxiliary component includes a fixed frame 4 arranged in the box body 1, a side plate 26 is slidably clamped on one side of the box body 1, the side plate 26 is connected to the fixed frame 4 close to the side of the box body 1, a plurality of slots 28 are provided in the fixed frame 4, each slot 28 is clamped with a culture bottle 13, one end of the culture bottle 13 is rotatably clamped with a sealing cover 3, a limiting groove 36 is provided in the box body 1, a sliding plate 24 slides in the limiting groove 36, and a clamping component is provided on the sliding plate 24.

[0020] like Figure 3 As shown, a connecting rod 14 is rotatably connected to the sliding plate 24, a connecting shaft 19 is provided on one side of the connecting rod 14, a coil spring is provided at the hinge between the connecting rod 14 and the sliding plate, a motor 8 is fixedly connected to the connecting rod 14, and a connecting frame 9 is rotatably connected to the output rod of the motor 8.

[0021] like Figure 2 and Figure 3 As shown, the clamping assembly includes a pair of clamps 11 slidably clamped on the connecting frame 9, the two clamps 11 are arranged opposite to each other, the two clamps 11 are connected by a first spring 10, chamfers 12 are arranged on opposite sides of the two clamps 11, and the clamps 11 are clamped on the outside of the sealing cover 3.

[0022] like Figure 3 As shown, a control component is provided on the sliding plate 24, and the control component includes a longitudinal rod 23 provided on the sliding plate 24, a connecting plate 21 is passed through the longitudinal rod 23, the bottom end of the connecting plate 21 is connected to the top wall of the sliding plate 24 through a third spring 22, and a sliding frame 20 is connected to the top of the connecting plate 21, and the sliding frame 20 is clamped on the outside of the connecting shaft 19.

[0023] like Figures 1 to 3As shown, the top of the sliding frame 20 is connected to the button 17 through the pressure rod 18, the top of the sliding plate 24 is connected to the moving frame 16, the moving frame 16 is slidably carded in the sliding groove 5 opened at the top of the box body 1, one end of the moving frame 16 is connected to the microscope, and the top of the box body 1 is provided with an observation window 6, the setting direction of the observation window 6 is the same as the extension direction of the limit groove 36, and the width of the pressure rod 18 is slightly narrower than that of the moving frame 16.

[0024] like Figure 1 , Figure 3 and Figure 5 As shown, a plurality of pressing buttons 35 are slidably clamped in the limit groove 36, and the inner bottom wall of the pressing button 35 is connected to the inner bottom wall of the box body 1 through the second spring 15. The number of the pressing buttons 35 is equal to the number of the clamping slots 28. The pressing button 35 is connected to a connecting frame 34 through one end, and the top of the connecting frame 34 is rotatably connected to a support plate 32. A pair of side grooves 33 are opened on the support plate 32, and the support plate 32 is clamped on the bottom wall of the box body 1 through the side grooves 33.

[0025] like Figure 5 As shown, a groove 29 is formed at the bottom end of the inner wall of each slot 28, and a support rod 30 is rotatably provided on the bottom wall of the slot 28. The upper surface of the support rod 30 is flush with the inner bottom wall of the slot 28, and the bottom end of the support rod 30 is connected to a bottom rod 31, and the bottom rod 31 and the support plate 32 are arranged in the same vertical plane.

[0026] like Figure 1 and Figure 3 As shown, a collecting box 2 is arranged on the inner bottom wall of the box body 1, the opening of the collecting box 2 is aligned with one end of the opening of the culture bottle 13, and a plurality of nozzles are arranged on the top of the collecting box 2, and the nozzles are aligned with the culture bottle 13.

[0027] like Figure 1 , Figure 2 and Figure 4 As shown, a telescopic rod 7 is provided at one side of the box body 1, and the top end of the output rod of the telescopic rod 7 contacts the bottom surface of the side plate 26.

[0028] The push button 35 can help the experimenter to pour the liquid in the culture bottle 13. When the liquid needs to be poured, the telescopic rod 7 is shortened, and the side plate 26 contacted by the top of the output rod end of the telescopic rod 7 will drive the fixed frame 4 to move downward. At this time, the support rod 30 rotated and clamped on the bottom wall of the slot 28 will move downward synchronously with the bottom rod 31. When the sliding plate 24 moves to one side of the culture bottle 13, it will simultaneously press down the push button 35 clamped in the limit groove on one side of the sliding plate 24, and the push button 35 will compress the second spring 15 and drive the push button When the connecting frame 34 connected with the connecting frame 35 moves down, the bottom rod 31 connected with the connecting frame 34 rotates to deflect upward on the bottom wall of the box body 1. When the support rod 30 and the bottom rod 31 move down synchronously, the bottom rod 31 will drive the support rod 30 to deflect upward due to the squeezing with the support plate 32, so that the culture bottle 13 stuck in the slot 28 deflects toward one end of the collecting box 2, and the culture bottle 13 will pour out the supernatant therein for subsequent passage. When liquid needs to be added, the telescopic rod 7 is reset and the nozzle is opened to pump liquid.

[0029] like Figure 1 and Figure 4 As shown, a partition 25 is provided in the box body 1, and a refrigeration chamber 27 is provided on one side of the partition 25; the refrigeration chamber 27 can store cells that are over-proliferated and cultured.

[0030] The specific usage and function of this embodiment are as follows: The auxiliary components provided in the present invention can assist the experimenter to add culture medium, digestive fluid and other experimental fluids into the culture bottle 13 when the cell density in the culture bottle 13 reaches the critical density of passage, so as to improve the separation efficiency. When using the device for cell culture, after the tumor cells are inoculated into the culture bottle 13, the culture bottle 13 is inserted into the card slot 28 provided in the fixed frame 4, and the insulation-related components in the box 1 are started to culture the tumor cells in the incubator at a constant temperature, and the sliding plate 24 provided in the limit groove is translated. Since one end of the mobile frame 16 connected to the top of the sliding plate 24 is connected to a microscope, and an observation window 6 is provided at the top of the box 1, and the setting direction of the observation window 6 is the same as the extension direction of the limit groove, the growth of the tumor cells in the box 1 can be observed through the observation window 6 during the translation of the sliding plate 24; When it is observed that the cell growth density in any culture bottle 13 reaches the critical value for passage, the button 17 connected to the pressure rod 18 is pressed down, and the sliding frame 20 connected to the bottom end of the pressure rod 18 drives the connecting shaft 19 clamped therein to move. Since the connecting rod 14 where the connecting shaft 19 is located is rotated and clamped on the sliding plate 24, the pressure rod 18 moves downward, driving the connecting rod 14 to rotate along the sliding plate 24, so that the clamping assembly on the connecting rod 14 is close to the sealing cover 3 set on the outside of the culture bottle 13; When the clamping assembly approaches the sealing cover 3, the clamp 11 will touch and squeeze the sealing cover 3 through the chamfer 12 set on the opposite side. At this time, the clamps 11 will move away from each other and stretch the first spring 10 until the clamp 11 is clamped to the outside of the side wall of the sealing cover 3. At this time, starting the motor 8 can make the clamp 11 drive the sealing cover 3 to rotate. After opening the sealing cover 3, release the button 17, and the pressure rod 18 will be reset under the elastic force of the third spring 22. At the same time, the pressure rod 18 will drive the connecting rod 14 to reset, so that the sealing cover 3 clamped on the connecting rod 14 will be separated from the opening of the culture bottle 13, which is convenient for the experimenter to add or pour liquid out.

[0031] The above is only an embodiment of the present invention, and the common knowledge such as the known specific technical solutions and / or characteristics in the solution is not described in detail here. It should be pointed out that for those skilled in the art, without departing from the technical solution of the present invention, several modifications and improvements can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. An integrated esophageal cancer cell culture and separation system, characterized by: The invention comprises a box body (1) and an auxiliary component, wherein the auxiliary component comprises a fixed frame (4) arranged in the box body (1), a side plate (26) is slidably clamped on one side of the box body (1), the side plate (26) is connected to the fixed frame (4) near the side of the box body (1), a plurality of clamping slots (28) are provided in the fixed frame (4), each of the clamping slots (28) is clamped with a culture bottle (13), one end of the culture bottle (13) is rotatably clamped with a sealing cover (3), a limiting groove (36) is provided in the box body (1), a sliding plate (24) slides in the limiting groove (36), and a clamping component is provided on the sliding plate (24).

2. The integrated esophageal cancer cell culture and separation system according to claim 1, characterized in that: The sliding plate (24) is rotatably connected to a connecting rod (14), a connecting shaft (19) is provided on one side of the connecting rod (14), a coil spring is provided at a hinge between the connecting rod (14) and the sliding plate, a motor (8) is fixedly connected to the connecting rod (14), and a connecting frame (9) is rotatably connected to an output rod of the motor (8).

3. The integrated esophageal cancer cell culture and separation system according to claim 2, characterized in that: The clamping assembly comprises a pair of clamps (11) slidably mounted on the connection frame (9), the two clamps (11) being arranged opposite to each other, the two clamps (11) being connected via a first spring (10), chamfers (12) being arranged on opposite sides of the two clamps (11), and the clamps (11) being mounted on the outside of the sealing cover (3).

4. The integrated esophageal cancer cell culture and separation system according to claim 3, characterized in that: The sliding plate (24) is provided with a control component, the control component comprising a longitudinal rod (23) provided on the sliding plate (24), a connecting plate (21) passing through the longitudinal rod (23), the bottom end of the connecting plate (21) being connected to the top wall of the sliding plate (24) via a third spring (22), the top end of the connecting plate (21) being connected to a sliding frame (20), the sliding frame (20) being clamped on the outside of the connecting shaft (19).

5. The integrated esophageal cancer cell culture and separation system according to claim 4, characterized in that: The top of the sliding frame (20) is connected to a button (17) via a pressure rod (18); the top of the sliding plate (24) is connected to a movable frame (16); the movable frame (16) is slidably mounted in a sliding groove (5) provided at the top of the box body (1); one end of the movable frame (16) is connected to a microscope; an observation window (6) is provided at the top of the box body (1); the observation window (6) is arranged in the same direction as the extending direction of the limiting groove (36); and the width of the pressure rod (18) is slightly narrower than that of the movable frame (16).

6. The integrated esophageal cancer cell culture and separation system according to claim 5, characterized in that: A plurality of pressing buttons (35) are slidably mounted in the limiting groove (36); the inner bottom wall of the pressing button (35) is connected to the inner bottom wall of the box body (1) via a second spring (15); the number of the pressing buttons (35) is equal to the number of the locking grooves (28); one end of the pressing button (35) is connected to a connecting frame (34); the top end of the connecting frame (34) is rotatably connected to a support plate (32); a pair of side grooves (33) are formed on the support plate (32); the support plate (32) is mounted on the bottom wall of the box body (1) via the side grooves (33).

7. The integrated esophageal cancer cell culture and separation system according to claim 6, characterized in that: A groove (29) is provided at the bottom end of the inner wall of each of the slots (28); a support rod (30) is rotatably provided on the bottom wall of the slots (28); the upper surface of the support rod (30) is flush with the inner bottom wall of the slots (28); the bottom end of the support rod (30) is connected to a bottom rod (31); and the bottom rod (31) and the support plate (32) are arranged in the same vertical plane.

8. The integrated esophageal cancer cell culture and separation system according to claim 7, characterized in that: A collecting box (2) is arranged on the inner bottom wall of the box body (1), the opening of the collecting box (2) is aligned with one end of the opening of the culture bottle (13), and a plurality of nozzles are arranged on the top of the collecting box (2), and the nozzles are aligned with the culture bottle (13).

9. The integrated esophageal cancer cell culture and separation system according to claim 8, characterized in that: A telescopic rod (7) is provided on one side of the box body (1), and the top end of the output rod of the telescopic rod (7) contacts the bottom surface of the side plate (26).

10. The integrated esophageal cancer cell culture and separation system according to claim 9, characterized in that: A partition (25) is provided in the box body (1), and a refrigeration compartment (27) is provided on one side of the partition (25).

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