EMS-ARTP compound mutation chlorella pyrenoidosa mutant strain and application thereof

The Chlorella mutant strain Y11 obtained through EMS-ARTP complex mutagenesis technology was heterotrophic cultured under dark conditions, solving the problem of low protein content in the existing technology, achieving the characteristics of high-yield protein and carotenoid-rich, and providing high-quality algae species for fermentation and production of high-quality microalgae proteins.

CN119709412APending Publication Date: 2025-03-28SOUTH CHINA UNIV OF TECH
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Patent Information

Application Number
CN202411699947.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-26
Publication Date
2025-03-28

AI Technical Summary

Technical Problem

The existing protein Chlorella nucleus has a low protein content in high-density fermentation, and the algae are dark green and have high lipid content, which leads to its low commercial application as a replacement protein.

Method used

Through EMS-ARTP complex mutagenesis technology, a Chlorella nucleus mutant strain Y11 was obtained. The algae strain was heterotrophic in dark conditions, with protein yield reaching 5.04 g/L, and the biomass, protein content and yield significantly improved.

Benefits of technology

The protein yield and biomass of the mutant strain Y11 have significantly improved, with a protein content of 55.54% DW, an amino acid score of 94.41, and a carotenoid content has also increased significantly, making it suitable for large-scale industrial production.

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Abstract

The invention discloses an EMS-ARTP compound mutation chlorella pyrenoidosa mutant strain and an application thereof. The preparation method comprises the following steps: mutagenizing a chlorella pyrenoidosa mutant strain CX41 by adopting an ethyl methanesulfonate (EMS) and normal-pressure and room-temperature plasma (ARTP) composite technology, screening and verifying to obtain a golden yellow chlorella pyrenoidosa mutant strain, naming the golden yellow chlorella pyrenoidosa mutant strain as chlorella pyrenoidosa Y11, and screening and verifying the golden yellow chlorella pyrenoidosa Y11 to obtain the chlorella pyrenoidosa Y11. The strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on the fifth floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou on October 23, 2024, and the preservation number is GDMCC No: 65324. The chlorella pyrenoidosa mutant strain Y11 has the characteristics of high yield of protein and rich carotenoids, and can be used for fermentation production of microalgae protein and carotenoids.
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