Application of schizochytrium limacinum mutant strain and grease produced by schizochytrium limacinum mutant strain in microcapsules

The ion-beam microbial mutagenesis breeding technology was used to screen out the schizophrenia mutant strain CABIO-A-2-VII, which solved the metabolic bottleneck problem of microbial fermentation and production of EPA and DHA, achieved high content of oil and fat, and significantly improved the quality of oil and fat in microcapsule applications.

CN119709431AActive Publication Date: 2025-03-28CABIO BIOTECH (WUHAN) CO LTD
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Patent Information

Application Number
CN202411966768.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-30
Publication Date
2025-03-28
Estimated Expiration
2044-12-30

AI Technical Summary

Technical Problem

When the prior art uses microbial fermentation to produce omega-3 series polyunsaturated fatty acids such as EPA, there is a metabolic bottleneck in the fatty acid synthesis pathway, which limits the content of EPA and DHA, and the application effect of oils and fats in microcapsules is not good.

Method used

Through ion beam microbial mutagenesis breeding technology, a schizochytrium mutant strain CABIO-A-2-VII was screened. During the fermentation process, the EPA content of this strain was greatly increased, the C14:0 content was high, the DHA content was ≥40%. The oil produced was low in surface oil content and peroxide value and had no fishy smell when preparing microcapsules.

Benefits of technology

Under conventional fermentation process, the DHA content in the oil and fat is ≥40%, the EPA content in the oil and fat is >2%, and the C14:0≥12%, and the embedding effect and quality of the oil and fat in the microcapsules are improved.

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Abstract

The invention provides a schizochytrium limacinum mutant strain and application thereof. The schizochytrium limacinum mutant strain is a schizochytrium limacinum mutant strain CABIO-A-2-VII, the preservation number of the schizochytrium limacinum mutant strain is CCTCC M 20242741, and the schizochytrium limacinum mutant strain is preserved in the China Center for Type Culture Collection. The EPA content in polyunsaturated fatty acid grease obtained by fermentation of the schizochytrium limacinum mutant strain CABIO-A-2-VII provided by the invention is greatly increased, the C14: 0 content is higher, the DHA content in grease obtained by using the strain under a conventional fermentation process is greater than or equal to 40%, the EPA content is greater than 2%, and the C14: 0 is greater than or equal to 12% (the C14: 0 can reach 19% under an optimal fermentation process).
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