A phosphorus-dissolving and deodorizing bactericide for promoting compost maturity, its preparation method and application

Abstract

The present invention discloses a compost-promoting, phosphorus-dissolving, and deodorizing bacterial agent, as well as its preparation method and application, belonging to the technical field of bio-agriculture and related industries. The bacterial agent is composed of the following strains in a mass ratio: Bacillus licheniformis DY-1: Paenibacillus zeae C-A: Bacillus chestnutii SC = 1.5:(1-3):(1.5-4); the viable cell count of each strain is ≥5×10 8 CFU / g; the total viable count of the bacterial agent is ≥1×10 9 CFU / g. Its preparation method includes the steps of (1) strain activation, (2) strain expansion culture, (3) bacterial powder preparation, and (4) strain compounding. Its application in clean composting of organic waste. The present invention has the following advantages: not only do the three bacterial strains in the microbial agent not produce antagonism, but they can also significantly accelerate the conversion of insoluble phosphorus, promote decomposition, and enhance the removal of NH3 and H2S.

Description

Technical Field

[0001] The invention belongs to the technical field of bio-agriculture and related industries, and particularly relates to a compost-promoting, phosphorus-dissolving and deodorizing bacterial agent, a preparation method and an application thereof. Background Art

[0002] Composting is a primary method for effectively treating and utilizing organic waste. It converts waste into organic fertilizer and activates insoluble nutrients like phosphorus into a form usable by crops. However, the composting process can also produce ammonia and hydrogen sulfide, causing nutrient loss and environmental pollution. Therefore, accelerating composting and promoting the conversion of insoluble nutrients like phosphorus while reducing the emission of malodorous gases like ammonia and hydrogen sulfide are key to achieving high-quality and clean composting.

[0003] The main treatment technologies for organic waste at home and abroad include physical, chemical and biological deodorization technologies. Currently, biotechnology is often used for the clean production of organic fertilizers, and the generation of harmful gases is reduced by adding microbial agents. The existing technology focuses more on single functions such as deodorization, and there are fewer agents that can solve the problems of high quality and cleanliness at the same time. In addition, the few existing multifunctional agents are usually directly compounded by multiple single-function strains, and the synergistic or antagonistic effects of strains are rarely considered, resulting in a better composting effect in the early stage of composting, but limited nutrient conversion and deodorization effects in the middle and late stages. Therefore, isolating and screening high-efficiency composting, phosphorus-dissolving and deodorizing strains, compounding them according to the interaction relationship between strains, and optimizing their compounding ratio can be an effective way to synthesize high-efficiency multifunctional composite bacterial agents. Summary of the Invention

[0004] The first purpose of the present invention is to disclose a compost-promoting, phosphorus-dissolving and deodorizing bacterial agent.

[0005] The second object of the present invention is to disclose a method for preparing the above-mentioned deodorizing bacterial agent.

[0006] The third object of the present invention is to disclose the application of the above-mentioned deodorizing bacteria agent.

[0007] The purpose of the present invention is achieved through the following technical solutions:

[0008] A compost-promoting, phosphorus-dissolving and deodorizing bacterial agent, wherein: the bacterial agent is composed of Bacillus licheniformis DY-1 ( Bacillus licheniformis DY-1) CGMCC No.32099, Paenibacillus zeae CA ( Paenibacillus zeisoli CA) CGMCC No.32101 and Bacillus chestnutii SC ( Bacillus badius SC) CGMCC No.32100;

[0009] The mass ratio between the strains is: Bacillus licheniformis DY ( Bacillus licheniformis DY-1): Paenibacillus zeae CA ( Paenibacillus zeisoli CA): Bacillus chestnutii SC ( Bacillus badius SC) = 1.5: (1-3): (1.5-4); the number of viable cells of each strain ≥ 5 × 10 8 CFU / g;

[0010] The total viable count of the bacterial agent is greater than or equal to 10 9 CFU / g.

[0011] The method for preparing the compost-promoting, phosphorus-dissolving and deodorizing bacterial agent described in the above technical solution comprises the following steps:

[0012] (1) Strain activation:

[0013] Bacillus licheniformis DY-1, Paenibacillus zeae CA, and Bacillus chestnut brown SC were inoculated into LB liquid medium respectively and cultured to the logarithmic growth phase to obtain seed solutions of Bacillus licheniformis DY-1, Paenibacillus zeae CA, and Bacillus chestnut brown SC;

[0014] (2) Expansion culture of bacteria:

[0015] The seed liquid of Bacillus licheniformis DY-1, Paenibacillus zeae CA, and Bacillus chestnut brown SC obtained in step (1) was transferred to a new LB liquid culture medium at an inoculum volume of 10% (v / v), and the culture was expanded to obtain the corresponding bacterial liquid;

[0016] (3) Preparation of bacterial powder:

[0017] The bacterial suspensions of Bacillus licheniformis DY-1, Paenibacillus zeae CA, and Bacillus chestnutii SC obtained in step (2) were centrifuged at 6000 rpm for 5 minutes at 4°C to collect bacterial precipitates, which were freeze-dried to prepare bacterial powders of the three strains;

[0018] (4) Bacterial strain compounding:

[0019] The number of live bacteria contained in the bacterial powders of Bacillus licheniformis DY-1, Paenibacillus zeae CA and Bacillus chestnut brown SC obtained in the detection step (3) is mixed according to the mass ratio of Bacillus licheniformis DY-1, Paenibacillus zeae CA and Bacillus chestnut brown SC of 1.5:(1-3):(1.5-4), thereby obtaining a compost-promoting, phosphorus-dissolving and deodorizing bacterial agent.

[0020] The preparation method described in the above technical solution, wherein: the culture conditions in step (1) and step (2) are:

[0021] Bacillus licheniformis DY-1 was cultured at 30°C and 180 rpm in a shaking incubator for 13–15 h;

[0022] Paenibacillus zeae CA was cultured at 30°C and 180 rpm in a shaking incubator for 18–20 h;

[0023] Bacillus chestnutii SC was cultured at 30°C with a shaker at 180 rpm for 16-18 hours.

[0024] The preparation method described in the above technical solution, wherein: the number of viable bacteria in the bacterial powder in step (4) is:

[0025] The number of viable cells of each strain was ≥5×10 8 CFU / g;

[0026] The number of viable bacteria in the mixed agent is ≥10 9 CFU / g.

[0027] The above technical solution is used to apply the composting-promoting, phosphorus-dissolving and deodorizing bacterial agent in the clean composting of organic waste.

[0028] Bacillus licheniformis DY-1 was screened from mushroom residue and grew on beef extract peptone agar medium. The colonies were light yellow, opaque, oval, slightly raised, single or paired, rod-shaped, and had spores.

[0029] Paenibacillus zeae CA was screened from mushroom residue and grew on inorganic phosphorus culture medium. The colonies were white, translucent, round, slightly raised, and the bacteria were monomeric, rod-shaped, and had spores.

[0030] Bacillus chestnutii SC was screened from livestock and poultry feces and grew on beef extract peptone agar medium. The colonies were white, opaque, round or nearly round, slightly protruding, smooth, rod-shaped, and had spores.

[0031] The present invention has the following beneficial effects:

[0032] 1. The present invention screened and obtained a strain of Bacillus chestnut brown SC, which has significantly improved the effect of removing NH3 and H2S compared with existing Bacillus.

[0033] 2. The present invention screened and obtained a strain of Bacillus licheniformis DY-1, which has excellent phosphate solubilization and cellulose degradation capabilities.

[0034] 3. The present invention screened and obtained a strain of Paenibacillus zeae CA, which has excellent phosphate solubilization ability.

[0035] 4. The present invention is to Bacillus licheniformis DY-1 ( Bacillus licheniformis DY-1), Paenibacillus zeisoli CA, Bacillus chestnutii SC ( Bacillus badiusThe results showed that the three strains of bacteria not only did not produce antagonism among each other, but also significantly accelerated the conversion of insoluble phosphorus, promoted decomposition, and enhanced the removal of NH3 and H2S. After actual testing, the phosphorus solubility capacity of the three strains after compounding could reach up to 372.11 mg / L, the cellulase activity could reach up to 15.76 U / mL, and the ammonia and hydrogen sulfide removal rates could reach 83.68-84.58% and 74.67-75.35%, respectively.

[0036] Strain deposit information:

[0037] 1. The present invention Bacillus licheniformis DY-1, classified as Bacillus licheniformis Bacillus licheniformis It has been deposited in the General Microbiology Center of China Culture Collection of Microorganisms (CGMCC). The address of the deposit unit is: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The deposit number is CGMCC No.32099 and the deposit date is September 29, 2024.

[0038] 2. The present invention Bacillus badius SC, classified as Bacillus chestnutus Bacillus badius It has been deposited in the General Microbiology Center of China Culture Collection Administration (CGMCC). The address of the deposit unit is: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The deposit number is CGMCC No. 32100 and the deposit date is September 29, 2024.

[0039] 3. The Paenibacillus zeisoli CA of the present invention is classified as Paenibacillus zeisoli and has been deposited in the General Microbiology Center of the China Culture Collection Administration of Microorganisms (CGMCC). The deposit address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The deposit number is CGMCC No. 32101 and the deposit date is September 29, 2024. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 It is the process of strain screening and composite bacterial agent preparation;

[0041] Figure 2 is the evolutionary tree of strain Bacillus licheniformis DY-1;

[0042] Figure 3 is the evolutionary tree of strain Paenibacillus zeae CA;

[0043] Figure 4 is the evolutionary tree of strain Bacillus chestnutii SC;

[0044] Figure 5 These are the results of pairwise antagonism experiments between strains. DETAILED DESCRIPTION

[0045] To facilitate understanding of the technical solution of the present invention, the following further describes a compost-promoting, phosphorus-dissolving, and deodorizing bacterial agent for composting, and its preparation method and application in conjunction with specific embodiments.

[0046] The process of strain screening and composite bacterial agent preparation is shown in Figure 1 The specific preparation process is as described in Example 1 and Example 2.

[0047] Example 1: Isolation of strains:

[0048] Take 10 g of mushroom residue and cow dung compost, put them into 50 ml of sterilized water, shake at 30°C and 180 rpm for 30 minutes, let it stand for 30 minutes, take 1 mL of the supernatant, dilute it to 10 with sterilized water, and add 10 ml of the supernatant. -1 , make 10-fold gradient dilutions continuously, aspirate 0.5 mL of the diluted suspension and spread it on beef extract peptone agar medium, inorganic phosphate medium and CMC-Na medium, culture at 30℃ for 24-36 hours, and select strains with different morphologies for streak culture and low-temperature storage according to the morphology and growth of the strains.

[0049] The culture medium and its formula in the above embodiment are as follows:

[0050] Beef extract peptone agar medium: peptone: 10 g, beef extract: 5 g, NaCl: 5 g, agar: 20 g, distilled water: 1000 mL, pH: 7.2;

[0051] Inorganic phosphorus medium: glucose: 10 g, (NH4)2SO4: 0.5 g, NaCl: 0.2 g, KCl: 0.2 g, MgSO4·7H2O: 0.1 g, MnSO4·H2O: 0.002 g, FeSO4·7H2O: 0.002 g, hydroxyapatite: 5 g, bromocresol purple: 0.05 g, agar: 20 g, distilled water: 1000 mL, pH: 7.0-7.5;

[0052] CMC-Na medium: CMC-Na: 15 g, MgSO4·7H2O: 0.5 g, yeast extract: 1.0 g, KH2PO4: 1.0 g, NH4NO3: 1.0 g, agar: 20 g, distilled water: 1000 mL, pH: 6.8-7.2.

[0053] Example 2: Screening and identification of strains:

[0054] 1. Screening of strains:

[0055] The strains were screened by measuring the release of ammonia and hydrogen sulfide, phosphate solubilization ability, and cellulase activity. The specific steps are as follows:

[0056] 1.1. Seed solution preparation:

[0057] Use a sterile inoculating loop to pick a single colony of the strain that grows well on the solid culture medium from the slant, inoculate it into LB liquid culture medium, place it in a shaker at 30°C and 180 rpm, and culture it for 24 hours to prepare the seed solution.

[0058] 1.2. Screening of deamination strains:

[0059] The seed solution was inoculated into 100 mL of denitrifying bacteria screening liquid medium at a 10% (v / v) inoculum volume. The blank group was inoculated with an equal amount of sterilized seed solution medium. Three replicates were set up for each group. The culture was shaken and cultured for 48 hours under the same conditions as the seed solution preparation. The residual NH4 in the culture medium was detected by Nessler's reagent spectrophotometry. + -N, calculate NH4 + -N removal rate, screening out NH4 + -N removal rate of high strains.

[0060] 1.3. Screening of desulfurization strains:

[0061] The seed solution was inoculated into the desulfurization bacteria screening liquid medium at a 10% (v / v) inoculum volume. The blank group was inoculated with the same amount of sterilized seed solution medium. Three replicates were set up for each group. The culture was shaken at 30°C and 180 rpm for 8 hours. The remaining S in the culture medium was detected by methylene blue spectrophotometry. 2- , calculate S 2- Removal rate, screening out S 2- Bacteria with high removal rate.

[0062] 1.4. Screening of phosphate-solubilizing strains:

[0063] Inoculate the seed solution at a 10% (v / v) inoculum into an inorganic phosphorus liquid medium and sterilize at 121°C for 20 minutes. Aseptically operate. A blank control without inoculation is used. Incubate on a shaker at 30°C and 180 rpm for 5-7 days. Determine the available phosphorus content using the molybdenum antimony colorimetric method.

[0064] 1.5. Screening of cellulose-degrading bacteria:

[0065] The seed liquid was inoculated into CMC-Na liquid culture medium at a 10% (v / v) inoculation rate, and cultured continuously in a 30°C constant temperature shaker at 180 rpm for 7 days. The cellulase activity was then determined using the DNS method.

[0066] The culture medium and formula used in the above screening steps are as follows:

[0067] Denitrifying bacteria liquid culture medium: (NH4)2SO4: 1.0 g / L, CH3COONa: 10.0 g / L, Weiss salt solution: 50 mL, sterilize at 121℃ for 15 minutes.

[0068] Desulfurization bacteria liquid culture medium: NaS·9H2O: 0.8 g / L, CH3COONa: 10.0 g / L, K2HPO4: 0.5 g / L, MgCl2: 0.2 g / L, NaHCO3: 5.0 g / L, (NH4)2SO4: 0.6 g / L, sterilized at 121°C for 15 minutes. Dissolve NaS·9H2O in an appropriate amount of water, filter through a 0.22 μm filter in a clean bench, and mix with the other sterilized solutions.

[0069] Inorganic phosphorus medium: glucose: 10 g, (NH4)2SO4: 0.5 g, NaCl: 0.2 g, KCl: 0.2 g, MgSO4·7H2O: 0.1 g, MnSO4·H2O: 0.002 g, FeSO4·7H2O: 0.002 g, Ca3(PO4)2: 5 g, bromocresol purple: 0.05 g, distilled water: 1000 mL, pH: 7.0-7.5

[0070] CMC-Na medium: CMC-Na: 15 g, MgSO4·7H2O: 0.5 g, yeast extract: 1.0 g, KH2PO4: 1.0 g, NH4NO3: 1.0 g, distilled water: 1000 mL, pH: 6.8-7.2.

[0071] Following the above screening steps, three strains with strong cellulose degradation, phosphate solubilization, and deamination and desulfurization capabilities were obtained, numbered DY-1, CA, and SC. The decay-promoting, phosphate solubilizing, and deodorizing abilities of each strain are shown in Table 1:

[0072] Table 1 The ability of each strain to promote decay, dissolve phosphate and deodorize

[0073]

[0074] 2. Identification of bacterial species:

[0075] 2.1 Physiological and biochemical tests:

[0076] 2.1.1. Inoculate the three strains obtained in step 1 onto the culture medium and culture at 30°C for 48 hours. Observe and record the growth and morphology of the colonies. Observe and record the bacterial morphology under a microscope.

[0077] The strain DY-1 was screened from mushroom residue and grew on beef extract peptone agar medium. The colonies were light yellow, opaque, oval, slightly convex, and single or paired, rod-shaped, with spores.

[0078] Strain CA was screened from mushroom residue and grew on inorganic phosphorus medium. The colonies were white, translucent, round, slightly convex, and the bacteria were monomeric, rod-shaped, and had spores.

[0079] The strain SC was screened from a cow dung pile and grew on beef extract peptone agar medium. The colonies were white, opaque, round or nearly round, slightly protruding, smooth, rod-shaped, and had spores.

[0080] 2.1.2. Physiological and biochemical tests were performed on each bacterium using a multiphase bacterial identification method. The specific procedures are as follows:

[0081] (1) Reagent strips used:

[0082] API 50CH reagent strips, API 20E reagent strips

[0083] (2) Supporting culture medium:

[0084] API 50 CHB / E medium, API 0.85% NaCl medium

[0085] (3) Preparation of bacterial suspension:

[0086] Use an inoculation tool to pick an appropriate amount of colonies from the plate and add them to API 50 CHB / E medium or API 0.85% NaCl medium, grind them carefully to make a uniform bacterial suspension.

[0087] (4) Prepare the training environment:

[0088] Prepare a culture box and pour about 5ml of distilled water into the honeycomb of the dish to create a wet chamber.

[0089] (5) Inoculation reagent strips:

[0090] Place the API 50CH or API 20E reagent strip in an incubation chamber and pipette the bacterial suspension into the vials of the strip. For the CIT, VP, and GEL vials in the API 20E reagent strip, ensure that the bacterial suspension fills the tube and cup. For the ADH, LDC, ODC, H2S, and URE vials, add mineral oil to create an anaerobic environment.

[0091] (6) Cultivation:

[0092] Place the inoculated reagent strips in an incubator and incubate at 36°C ± 2°C for 18-24 hours.

[0093] (7) Interpretation of results:

[0094] Observe the color changes of each tube on the test strip and refer to the test strip instructions to determine whether it is positive, weakly positive or negative.

[0095] The physiological and biochemical test results of the three strains obtained are shown in Table 2.

[0096] Table 2 Physiological and biochemical characteristics of strains

[0097]

[0098] Note: “+”: positive, “w”: weak positive, “-”: negative

[0099] 2.2 Molecular Validation

[0100] The three strains were inoculated into LB liquid medium and incubated at 30°C in a shaker for 24 hours. The cells were then harvested by centrifugation at 12,000 rpm. The genomes of strains DY-1, CA, and SC were extracted using the Ezup column-based bacterial genomic DNA extraction kit. PCR amplification was performed using primers 27F and 1492R. The nucleotide sequences of the primers are as follows:

[0101] 27F: 5'-AGAGTTTGATCCTGGCTCAG-3'

[0102] 1492R:5'-AGAGTTTGATCCTGGCTCAG-3'

[0103] After purification, the PCR product was sent to Shanghai Bioengineering Co., Ltd. for sequencing, and the sequencing results were compared with the sequences in the NCBI website database for homology analysis. Figure 2 The evolutionary tree of strain CA is shown in Figure 3 The evolutionary tree of strain SC is shown in Figure 4 shown.

[0104] Finally, the physiological and biochemical characteristics of the three strains were combined with molecular verification to confirm that the three strains involved in the present invention belong to Bacillus licheniformis ( Bacillus licheniformis ), Paenibacilluszeisoli, Bacillus chestnut brown ( Bacillus badius ).

[0105] Example 3: Compound of decomposition-promoting, phosphorus-dissolving and deodorizing bacteria agent:

[0106] 1. Antagonism experiment:

[0107] The three strains were tested for antagonism on solid culture medium using the filter paper method. The bacteria on the plate were the base bacteria, and the bacteria on the filter paper were the test bacteria. The growth of the base bacteria and the test bacteria was observed. If there is no inhibition zone between the two bacteria, it means there is no antagonism. Otherwise, it means there is antagonism between the strains. Figure 5 As shown, there was no antagonism among the three strains.

[0108] 2. Compounding of strains:

[0109] The three strains were inoculated into LB liquid medium and cultured in a shaking oven until the logarithmic growth phase to obtain seed solutions of Bacillus licheniformis DY-1, Paenibacillus zeae CA, and Bacillus chestnut brown SC; Bacillus licheniformis DY-1 was cultured in a shaking oven at 30°C and 180 rpm for 14 hours; Paenibacillus zeae CA was cultured in a shaking oven at 30°C and 180 rpm for 19 hours; and Bacillus chestnut brown SC was cultured in a shaking oven at 30°C and 180 rpm for 16 hours to obtain seed solutions of the three strains; the inoculation and expansion culture were followed by freeze-drying (the specific method was as follows: the seed solutions of Bacillus licheniformis DY-1, Paenibacillus zeae CA, and Bacillus chestnut brown SC were transferred to new LB liquid medium at an inoculum rate of 10% (v / v); Bacillus licheniformis DY-1 was cultured in a shaking oven at 30°C and 180 rpm for 13 hours; Paenibacillus zeae CA was cultured in a shaking oven at 30°C and 180 rpm rpm shaking culture for 20 hours; Bacillus chestnut brown SC was cultured at 30°C, 180 rpm shaking culture for 17 hours, and the corresponding bacterial liquids were expanded and cultured; the three bacterial liquids were centrifuged at 6000 rpm for 5 minutes at 4°C to collect the bacterial precipitates, freeze-dried, and the bacterial powders of the three strains were prepared. The bacterial powders of the three strains (viable cell count ≥ 5 × 10 8 The strains were prepared according to the following mass ratios: (Bacillus licheniformis DY-1: Paenibacillus zesneri CA: Bacillus chestnut brown SC) = A: (1.5:3:2); B: (1.5:4:1.5); C: (1.5:1:3); D: (1.5:1.5:4); E: (1.5:2:4); F: (1.5:1.5:3). Six combinations were inoculated into denitrifying bacteria liquid culture medium, desulfurizing bacteria liquid culture medium, inorganic phosphorus culture medium, and CMC-Na culture medium, respectively, for the determination of cellulase activity, phosphate solubilization capacity, ammonia removal rate, and hydrogen sulfide removal rate. The results are shown in Table 3. The results in Table 3 show that the phosphate solubilization ability of combinations A and B is higher than that of other combinations, and higher than the phosphate solubilization ability of the phosphate strain reported in patent CN117757671A; the deodorization ability of combinations C and D is higher than that of other combinations, and higher than the ammonia and hydrogen sulfide removal rate of the compound deodorizing bacteria agent in CN201811547950; the cellulase activity, phosphate solubilization and deodorization ability of combinations E and F are all higher.

[0110] Table 3 Effects of compound microbial agents on promoting decay, dissolving phosphorus and deodorizing

[0111]

[0112] The above description is only a preferred embodiment of the present invention and does not impose any formal or substantial limitation on the present invention. Any technician familiar with the present profession can make use of the technical content disclosed above without departing from the scope of the technical solution of the present invention, and any equivalent changes, modifications and evolutions made by them are equivalent embodiments of the present invention. At the same time, any equivalent changes, modifications and evolutions made to the above embodiments based on the essential technology of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A compost-promoting, phosphorus-dissolving and deodorizing bacterial agent, characterized in that: The bacterial agent is composed of Bacillus licheniformis ( Bacillus licheniformis )DY-1 CGMCC No.32099, Paenibacillus zeae ( Paenibacilluszeisoli ) C-ACGMCC No.32101 and Bacillus chestnutii ( Bacillus badius ) SC CGMCC No.32100; The mass ratio of each strain is: Bacillus licheniformis DY-1: Paenibacillus zeae CA: Bacillus chestnutii SC = 1.5: (1-3): (1.5-4); the number of viable cells of each strain is ≥ 5 × 10 8 CFU / g; The total viable count of the bacterial agent is greater than or equal to 10 9 CFU / g.

2. A method for preparing the compost-promoting, phosphorus-dissolving and deodorizing bacterial agent according to claim 1, comprising the following steps: (1) Strain activation: Bacillus licheniformis DY-1, Paenibacillus zeae CA, and Bacillus chestnut brown SC were inoculated into LB liquid medium respectively, and cultured to the logarithmic growth phase to obtain seed solutions of Bacillus licheniformis, Paenibacillus zeae CA, and Bacillus chestnut brown; (2) Expansion culture of bacteria: The seed liquid of Bacillus licheniformis DY-1, Paenibacillus zeae CA, and Bacillus chestnut brown SC obtained in step (1) was transferred to a new LB liquid culture medium at an inoculum volume of 10% v / v, and the culture was expanded to obtain the corresponding bacterial liquid; (3) Preparation of bacterial powder: The bacterial suspensions of Bacillus licheniformis DY-1, Paenibacillus zeae CA, and Bacillus chestnutii SC obtained in step (2) were centrifuged at 6000 rpm, and the bacterial precipitates were collected and freeze-dried to prepare bacterial powders of the three strains; (4) Bacterial strain compounding: The number of live bacteria contained in the bacterial powders of Bacillus licheniformis DY-1, Paenibacillus zeae CA and Bacillus chestnut brown SC obtained in step (3) is detected, and the mixture is mixed according to the mass ratio of Bacillus licheniformis DY-1, Paenibacillus zeae CA and Bacillus chestnut brown SC of 1.5:(1-3):(1.5-4) to obtain a composting-promoting, phosphorus-dissolving and deodorizing bacterial agent.

3. The preparation method according to claim 2, wherein: The culture conditions of step (1) and step (2) are: Bacillus licheniformis DY-1 was cultured at 30°C and 180 rpm in a shaking incubator for 13–15 h; Paenibacillus zeae CA was cultured at 30°C and 180 rpm in a shaking incubator for 18–20 h; Bacillus chestnutii SC was cultured at 30°C with a shaker at 180 rpm for 16-18 hours.

4. The preparation method according to claim 2, wherein: The number of viable bacteria in the bacterial powder in step (4) is: The number of viable cells of each strain was ≥5×10 8 CFU / g; The number of viable bacteria in the mixed agent is ≥10 9 CFU / g.

5. Use of the compost-promoting, phosphorus-dissolving and deodorizing bacterial agent according to claim 1 in clean composting of organic waste.

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