Kit for detecting FGFR2 (Fibroblast Growth Factor Receptor 2) gene fusion mutation in bile duct cancer
By developing a kit for detecting fusion mutations of FGFR2 gene in cholangiocarcinoma, the problem of limited detection targets is solved using digital PCR and universal linker design, and high sensitivity and high accuracy detection of multiple fusion genes is achieved.
CN119710001AActive Publication Date: 2025-03-28SHANGHAI LANWEI MEDICAL LAB CO LTD
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Patent Information
- Application Number
- CN202411747094.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-29
- Publication Date
- 2025-03-28
- Estimated Expiration
- 2044-11-29
AI Technical Summary
Technical Problem
Current conventional methods for detecting fusion genes using digital PCR can only detect known fusions and have limited detection targets.
Method used
A kit for detecting fusion mutations of FGFR2 gene in cholangiocarcinoma was developed. By combining a reverse transcription reagent and PCR expression reagent, a universal linker method based on digital PCR was designed to detect all fusions at one time at which the breakpoint is 3' end of exon FGFR217 was used.
Benefits of technology
High sensitivity and high accuracy detection of FGFR2 gene fusion mutations in cholangiocarcinoma is achieved, and about 150 fusion genes can be detected simultaneously, solving the problem of limited detection targets.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention discloses a kit for detecting FGFR2 (Fibroblast Growth Factor Receptor 2) gene fusion mutation in bile duct cancer, and develops a design method of a universal joint based on digital PCR (Polymerase Chain Reaction) by utilizing the characteristic that most breakpoints of FGFR2-driven fusion genes are positioned at the 3'end of an FGFR217 exon, and all fusion of which the breakpoints are the 3 'end of the FGFR217 exon can be detected at one time. In addition, a tail sequence Tail is specially designed for a fusion specific primer, and the purpose of inhibiting FGFR2WT cDNA amplification is achieved through complementation of the tail sequence Tail and a sequence, close to a breakpoint, of FGFR2WT exon18, so that the amplification efficiency of FGFR2fusion is greatly improved. The kit is convenient to operate, and has the characteristics of high sensitivity, high accuracy, low cost and the like.
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