Scylla paramamosain carapace length related SNP (Single Nucleotide Polymorphism) molecular marker, detection primer pair, kit and application

Through genome-wide correlation analysis, SNP sites related to dorsal carapace length on the chromosome 4 of the pseudo-crawan crawan were determined, and SNP molecular markers and detection tools were developed, which solved the problem of difficulty in identifying dorsal carapace length-related SNP markers in the existing technology, and achieved efficient evaluation and breeding of the dorsal carapace length traits of the blue crab, significantly accelerating the genetic improvement process.

CN119710031APending Publication Date: 2025-03-28JIMEI UNIV
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Patent Information

Application Number
CN202510057006.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-01-14
Publication Date
2025-03-28

AI Technical Summary

Technical Problem

The prior art has not yet successfully identified SNP markers directly related to the long trait of the dorsal carapace of the simile blue crab through GWAS analysis of whole genome resequencing technology, and it is difficult to evaluate and breed individual phenotypes in the early stages.

Method used

Through genome-wide association analysis, it was determined that the G>T mutation at locus 31207983 on chromosome 4 of the pseudo-caryng Crab was significantly correlated with the dorsal carapace length trait. SNP molecular markers related to dorsal carapace length were developed, and primer pairs and kits were designed to detect the SNP marker.

Benefits of technology

The efficient evaluation and breeding of the long traits of the dorsal carapace of the blue crab were achieved, and the long traits of the dorsal carapace were gradually improved through genome selection, which significantly accelerated the genetic improvement process of the blue crab and improved the economic benefits of the breeding industry.

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Abstract

The invention discloses a scylla paramamosain carapace length related SNP (Single Nucleotide Polymorphism) molecular marker, a detection primer pair, a kit and application. According to the SNP molecular marker, the 179th basic group from the 5'end of a nucleotide sequence shown in SEQ ID NO: 1 is G or T. The SNP molecular marker can predict the carapace length character of an individual in the early breeding stage, and is used for molecular marker-assisted selective breeding, improving the breeding efficiency and accuracy of scylla paramamosain, shortening the breeding period, reducing the breeding cost and improving the economic benefit.
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