Triglyceride quality control liquid and preparation method thereof

Abstract

The invention discloses a triglyceride quality control liquid and a preparation method thereof, the triglyceride quality control liquid comprises a triglyceride concentrated solution and a base solution containing a dispersing agent and an activating agent, the triglyceride concentrated solution is added into the base solution, and the mixture is homogenized and dispersed; the activating agent is a lipoprotein lipase activating agent; the volume ratio of the triglyceride concentrated solution to the basic solution is 1: (10-300). The triglyceride quality control liquid provided by the invention has good repeatability, excellent stability and long storage life, can be well applied to an electrochemical detection system, and ensures the accuracy of a quality control result; and the influence on the quality control test result of the detection system is smaller, the measured data is closer to the real value, and the method is more suitable for the electrochemical triglyceride test system.

Description

Technical Field

[0001] The present invention relates to the technical field of in vitro diagnostic reagents, and in particular to a triglyceride quality control solution and a preparation method thereof. Background Art

[0002] Triglycerides (TG), also known as neutral fat, are composed of three molecules of fatty acids and one molecule of glyceride, and are the main source of energy in the body. The level of triglycerides is not only positively correlated with total cholesterol (TC), body mass index (BMI) and blood glucose (GLU), but is also closely related to obesity. The detection of triglycerides in human blood is very important because its high concentration (normal range is 40-160 mg / d for men and 35-135 mg / dL for women) can lead to hyperlipidemia, which is one of the main manifestations of coronary heart disease. Timely detection helps diagnose and treat diabetes, kidney disease, liver obstruction and other diseases related to lipid metabolism and various endocrine disorders.

[0003] The quality control liquid is a solution containing the substance to be tested or a substance with the same properties as the substance to be tested, and its function is to confirm whether the function of the electrochemical detection system is normal. When there is a large deviation or a questionable result during the detection process, the detection result of the detection system on the quality control liquid can be used as a basis to confirm whether the detection system is in a normal detection state. The blood lipid composite quality control liquid of the prior art is only suitable for blood lipid analyzers and blood lipid analysis systems using photochemical principles. A blood filter membrane is attached to the reaction area. During the test, the triglyceride test strip of triglycerides in the plasma after the red blood cells are filtered through the blood filter membrane. Triglycerides are insoluble in water, and form emulsions in aqueous solutions and stratify and agglomerate. Therefore, it is necessary to use surfactants to dissolve triglycerides. However, surfactants have a certain effect on enzyme activity, and the quality control liquid prepared by this method is less sensitive to test strips based on electrochemical sensors and is not applicable.

[0004] Therefore, developing a quality control solution that can be used in triglyceride electrochemical detection systems has become an urgent problem that technicians in this field need to solve. Summary of the invention

[0005] In view of the deficiencies in the prior art, the present invention provides a triglyceride quality control solution and a preparation method thereof.

[0006] To achieve the above object, the technical solution adopted in the present invention is as follows: In one aspect, the present invention provides a triglyceride quality control solution, comprising a triglyceride concentrate and a base solution containing a dispersant and an activator, wherein the triglyceride concentrate is added to the base solution and dispersed homogenously; The activator is a lipoprotein lipase activator; The volume ratio of the triglyceride concentrate to the base liquid is 1:(10-300).

[0007] Preferably, the matrix of the triglyceride concentrate is bovine serum, and the concentration is 6000 mg / dL-8000 mg / dL.

[0008] Preferably, the concentration of the activator is 2 g / L-10 g / L.

[0009] Preferably, the concentration of the dispersant is 1 g / L-6 g / L.

[0010] Preferably, the base solution further comprises a buffer, a stabilizer and a preservative.

[0011] Preferably, the activator comprises one or more of ibromide, N-2-nitro-4-chlorophenyl-4-[(phosphonic acid diethyl ester) methyl] benzohydrazide, and N-2-tetrazolyl-4-chlorophenyl-4-[(phosphonic acid diethyl ester) methyl] benzamide.

[0012] Preferably, the dispersant includes one or more of triammonium citrate, ammonium polyacrylate, and sodium tripolyphosphate.

[0013] Preferably, the buffer is a buffer with a pH of 7, including one or more of PBS buffer, Mops buffer, HEPES buffer, TES buffer, and PIPES-sesquisodium salt, and the concentration of the buffer is 0.01 mol / L-0.2 mol / L.

[0014] Preferably, the stabilizer includes one or more of sucrose, trehalose, dextran and lactose, and the concentration of the stabilizer is 5 g / L-20 g / L.

[0015] Preferably, the preservative includes one or more of potassium sorbate, sodium azide, and Proclin 300, and the concentration of the preservative is 0.3 g / L-1 g / L.

[0016] Preferably, in the triglyceride quality control solution, the triglyceride concentration is 0.5 mmol / L-8.0 mmol / L.

[0017] In another aspect, the present invention provides a method for preparing the above-mentioned triglyceride quality control solution, comprising the following steps: Prepare the base liquid raw materials and triglyceride concentrate according to the proportion; Mixing base liquid raw materials to prepare base liquid; The triglyceride concentrate is added to the base solution, and the triglyceride quality control solution is obtained after homogenization and dispersion.

[0018] Preferably, the preparation method comprises the following steps: Prepare the base liquid raw materials and triglyceride concentrate according to the proportion; An activator, a dispersant, a stabilizer and a preservative are sequentially added into a buffer solution to prepare a base solution; Triglyceride concentrate was added to the base solution, and the triglyceride quality control solution was obtained after homogenization and dispersion at 25°C for 5 minutes.

[0019] Preferably, the speed of the homogenous dispersion is 150rpm-1440rpm; The homogenous dispersion is performed using a non-intrusive material homogenizer.

[0020] Compared with the prior art, the present invention has the following beneficial technical effects: The present invention obtains a triglyceride quality control liquid by configuring a base liquid containing a dispersant with a triglyceride concentrate. The dispersant is used to provide a larger molecular weight, and an adsorption layer is formed on the surface of the triglyceride, thereby providing a steric hindrance effect; and by controlling the rotation speed during the homogenization and dispersion process, the triglyceride is strongly treated by the shear and destructive force generated by the high-speed operation of the homogenizer rotor; the steric hindrance effect provided by the above-mentioned dispersant and the shear destructive force of the homogenizer can effectively prevent the particles in the triglyceride emulsion from aggregating and stratifying, so that the dispersed system remains in a stable state, so the triglyceride quality control liquid provided by the present invention has good repeatability and stability.

[0021] The present invention improves the stability of triglyceride quality control solution by using a stabilizer, so that the test results are more accurate, and also strengthens the storage stability of the triglyceride quality control solution; and then uses a preservative to avoid the generation of bacteria during the production and storage of the triglyceride quality control solution, thereby extending the shelf life of the triglyceride quality control solution. Therefore, the triglyceride quality control solution provided by the present invention has excellent stability, a long shelf life, and can be well applied to an electrochemical detection system to ensure the accuracy of the quality control results.

[0022] The activator used in the present invention is a lipoprotein lipase activator, which has strong biological activity, can keep lipoprotein lipase at a high catalytic efficiency, and can also reduce the influence of dispersants and other ingredients on enzyme activity; specifically, PPARα (a type of nuclear receptor superfamily transcription factor activated by ligand) is a key nuclear receptor that regulates the expression of lipoprotein lipase genes. Lipoprotein lipase activators promote the activation of transcription factor PPARα by activating the PPARα pathway, thereby regulating the expression of lipoprotein lipase and increasing the activity of lipoprotein protease. Lipoprotein lipase activators enhance the specific cutting of lipoprotein lipase on the ester bond of substrate triglycerides by activating the activity of lipoprotein lipase required for triglyceride hydrolysis, thereby accelerating the hydrolysis efficiency of triglycerides, thereby improving the detection efficiency of triglycerides and improving the accuracy of detection; and the activator also has a synergistic effect with the dispersant, which can jointly promote the hydrolysis of triglycerides. The triglyceride quality control solution provided by the present invention was tested using triglyceride test paper, and good sensitivity and linear response signals were obtained, indicating that the present invention can play a quality control role in the triglyceride test system.

[0023] Compared with the quality control solution in the prior art, the triglyceride quality control solution provided by the present invention has less impact on the quality control test results of the detection system, the measured data is closer to the true value, is more suitable for the electrochemical triglyceride test system, and has excellent stability and can be stored for a long time. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] Example 1 The triglyceride quality control solution provided in this embodiment includes a triglyceride concentrate and a base solution; The basic solution includes: 2g / L, 5g / L, 10g / L N-2-nitro-4-chlorophenyl-4-[(phosphonodiethyl)methyl]benzoylhydrazide; 0.05mol / L PBS buffer; 3g / L sodium tripolyphosphate; 10g / L trehalose; 0.5g / L potassium sorbate; The triglyceride concentrate is a Triglyceride concentrate produced by Medix Biocheica, with a concentration of 6000-8000 mg / dL; The base solution containing 2 g / L, 5 g / L and 10 g / L of N-2-nitro-4-chlorophenyl-4-[(diethylphosphonate)methyl]benzohydrazide was mixed with triglyceride concentrate at a volume ratio of 100:1; 50:1; 25:1; 12.5:1, respectively. After mixing, the mixture was homogenized and dispersed in a non-invasive material homogenizer at 500 rpm for 5 min at 25°C to obtain three triglyceride quality control solutions with four concentration levels of theoretical concentrations of 2 mmol / L (level 1), 4 mmol / L (level 2), 6 mmol / L (level 3) and 8 mmol / L (level 4).

[0026] Example 2 The triglyceride quality control solution provided in this embodiment includes a triglyceride concentrate and a base solution; The basic solution includes: 1g / L, 3g / L, 6g / L sodium tripolyphosphate; 5g / L N-2-nitro-4-chlorophenyl-4-[(phosphonodiethyl)methyl]benzoylhydrazide; 0.05mol / L Mops buffer; 10g / L trehalose; 0.5g / L potassium sorbate; The triglyceride concentrate is a Triglyceride concentrate produced by Medix Biocheica, with a concentration of 6000-8000 mg / dL; The base solution containing 1g / L, 3g / L and 6g / L of sodium tripolyphosphate was mixed with triglyceride concentrate in a volume ratio of 100:1; 50:1; 25:1; 12.5:1, respectively. After mixing, the mixture was homogenized and dispersed at 500rpm in a non-invasive material homogenizer at 25°C for 5 minutes to obtain three triglyceride quality control solutions with four concentration levels of theoretical concentrations of 2mmol / L (level 1), 4 mmol / L (level 2), 6 mmol / L (level 3) and 8mmol / L (level 4).

[0027] Example 3 The triglyceride quality control solution provided in this embodiment includes a triglyceride concentrate and a base solution; The basic solution includes: 5g / L, 10g / L, 20g / L trehalose; 5g / L N-2-nitro-4-chlorophenyl-4-[(phosphonodiethyl)methyl]benzoylhydrazide; 0.05mol / L PBS buffer; 3g / L sodium tripolyphosphate; 0.5g / L potassium sorbate; The triglyceride concentrate is a Triglyceride concentrate produced by Medix Biocheica, with a concentration of 6000-8000 mg / dL; The base solution containing 5 g / L, 10 g / L and 20 g / L of trehalose was mixed with triglyceride concentrate at a volume ratio of 100:1; 50:1; 25:1; 12.5:1, respectively. After mixing, the mixture was homogenized and dispersed at 500 rpm in a non-invasive material homogenizer at 25°C for 5 min to obtain three triglyceride quality control solutions with four concentration levels of theoretical concentrations of 2 mmol / L (level 1), 4 mmol / L (level 2), 6 mmol / L (level 3) and 8 mmol / L (level 4).

[0028] Example 4 The triglyceride quality control solution provided in this embodiment includes a triglyceride concentrate and a base solution; The basic solution includes: 0.3g / L, 0.5g / L, 1g / L potassium sorbate; 5g / L N-2-nitro-4-chlorophenyl-4-[(phosphonodiethyl)methyl]benzoyl hydrazide; 0.05mol / L PIPES-sesquisodium salt buffer; 3g / L sodium tripolyphosphate; 10g / L trehalose; The triglyceride concentrate is a Triglyceride concentrate produced by Medix Biocheica, with a concentration of 6000-8000 mg / dL; Base solutions containing 0.3 g / L, 0.5 g / L, and 1 g / L of potassium sorbate were mixed with triglyceride concentrate at a volume ratio of 100:1; 50:1; 25:1; 12.5:1, respectively. After mixing, the solutions were homogenized and dispersed at 500 rpm in a non-invasive material homogenizer at 25°C for 5 min to obtain three triglyceride quality control solutions with four concentration levels, namely, 2 mmol / L (level 1), 4 mmol / L (level 2), 6 mmol / L (level 3), and 8 mmol / L (level 4).

[0029] Example 5 The triglyceride quality control solution provided in this embodiment includes a triglyceride concentrate and a base solution; The basic solution includes: 5g / L N-2-nitro-4-chlorophenyl-4-[(phosphonodiethyl)methyl]benzoylhydrazide; 0.05mol / L PBS buffer; 3g / L sodium tripolyphosphate; 10g / L trehalose; 0.5g / L potassium sorbate; The triglyceride concentrate is a Triglyceride concentrate produced by Medix Biocheica, with a concentration of 6000-8000 mg / dL; The above-mentioned base liquid was mixed with triglyceride concentrate in a volume ratio of 100:1; 50:1; 25:1; 12.5:1. After mixing, the mixture was homogenized and dispersed in a non-invasive material homogenizer at 500 rpm for 5 minutes at 25°C to obtain a triglyceride quality control solution with four concentration levels of 2 mmol / L (level 1), 4 mmol / L (level 2), 6 mmol / L (level 3), and 8 mmol / L (level 4).

[0030] Example 6 The difference from Example 5 is that 5 g / L of ibromide is used instead of 5 g / L of N-2-nitro-4-chlorophenyl-4-[(diethylphosphonate)methyl]benzohydrazide, and the remaining ingredients and steps are the same as those in Example 5.

[0031] Example 7 The difference from Example 5 is that 2.5 g / L of ibromide and 2.5 g / L of N-2-tetrazolyl-4-chlorophenyl-4-[(diethylphosphonate)methyl]benzamide are used instead of 5 g / L of N-2-nitro-4-chlorophenyl-4-[(diethylphosphonate)methyl]benzohydrazide, and the remaining ingredients and steps are the same as those in Example 5.

[0032] Example 8 The difference from Example 5 is that 3 g / L of triammonium citrate is used instead of 3 g / L of sodium tripolyphosphate, and the remaining ingredients and steps are the same as those in Example 5.

[0033] Example 9 The difference from Example 5 is that 1.5 g / L of triammonium citrate and 1.5 g / L of ammonium polyacrylate are used instead of 3 g / L of sodium tripolyphosphate, and the remaining ingredients and steps are the same as those in Example 5.

[0034] Comparative Example 1 The difference from Example 5 is that 3 g / L sodium tripolyphosphate is not added, and the remaining ingredients and steps are the same as those of Example 5.

[0035] Comparative Example 2 The difference from Example 5 is that 5 g / L of N-2-nitro-4-chlorophenyl-4-[(diethylphosphonate)methyl]benzohydrazide is not added, and the remaining components and steps are the same as those in Example 5.

[0036] The accuracy of the triglyceride lipid control solution provided by the present invention was verified: The triglyceride quality control solutions of four concentration levels in Examples 1 to 9 and Comparative Examples 1 and 2 were divided into vials with a volume of 5 mL each, sealed and refrigerated at 2-8° C. The triglyceride quality control solutions of different concentration levels were taken with a pipette, and 500 μL of each triglyceride quality control solution was used as a sample. The triglyceride content in the sample was detected using a Mindray BS-360s biochemical analyzer and an electrochemical workstation, and the accuracy was evaluated. The test results are shown in Tables 1 and 2.

[0037] Table 1 Triglyceride quality control solution accuracy test table (Examples 1-4)

[0038] Table 2 Triglyceride quality control solution accuracy test table (Examples 5-9, Comparative Examples 1-2)

[0039] As can be seen from Table 1 and Table 2, the relative deviation between the biochemical mean of Examples 1 to 9 and the mean of the theoretical concentration of the examples is about ±4%, which is significantly better than Comparative Examples 1 and 2. This is because the lipoprotein lipase activator has a high biological activity, can catalyze the esterification of triglycerides, and accelerate the reaction rate of triglycerides; the dispersant provides a larger molecular weight, forms an adsorption layer on the surface of triglycerides, thereby providing a steric hindrance effect; and the shear and destructive force generated by the high-speed operation of the homogenizer rotor strongly treats the triglycerides; the steric hindrance effect provided by the dispersant and the shear destructive force of the homogenizer can effectively prevent the aggregation and stratification of particles in the triglyceride emulsion, so that the dispersed system remains in a stable state; the combination of a uniform and stable dispersed system and an accelerated reaction rate of triglycerides makes the triglyceride quality control solution provided by the present invention have excellent accuracy. That is, it shows that the synergistic effect of the lipoprotein lipase activator and the dispersant makes the triglyceride quality control solution have excellent accuracy.

[0040] The repeatability of the triglyceride lipid control solution provided by the present invention was evaluated: The triglyceride quality control solutions at four concentration levels in Examples 5 to 9 and Comparative Examples 1 and 2 were divided into vials with a volume of 5 mL each, sealed and refrigerated at 2-8°C. The triglyceride quality control solutions at different concentration levels were taken with a pipette, and 500 μL of each triglyceride quality control solution was used as a sample. The triglyceride content in the sample was detected using a Mindray BS-360s biochemical analyzer and an electrochemical workstation. The test kit was a triglyceride kit produced by Mindray. The triglyceride quality control solution was subjected to biochemical testing, and the current value AV was tested with an electrochemical workstation, and the coefficient of variation CV of the current value tested by the electrochemical workstation was calculated. The test results are shown in Table 3.

[0041] Table 3 Triglyceride quality control solution repeatability test table

[0042] As can be seen from Table 3, the CV of the triglyceride quality control solution provided by Examples 5 to 9 is kept below 4%, and the CVs of Examples 5 to 9 are similar, indicating that the triglyceride quality control solution has good repeatability; while the repeatability test deviations of Comparative Examples 1 and 2 are larger than those of Examples 5 to 9. This indicates that the triglyceride quality control solution prepared by the present invention can effectively ensure the repeatability of the quality control test results, which is the result of the combined action of the dispersant and the lipoprotein lipase activator in the triglyceride quality control solution provided by the present invention.

[0043] The triglyceride lipid control solution provided by the present invention is evaluated for linearity: The triglyceride quality control solutions at four concentration levels in Examples 5 to 9 and Comparative Examples 1 and 2 were divided into vials with a volume of 5 mL each, sealed and refrigerated at 2-8°C. 10 portions of triglyceride quality control solutions at different concentration levels were taken with a pipette, and 100 μL of triglyceride quality control solution per portion was used as a sample. The triglyceride concentration in the sample was detected using a Mindray BS-360s biochemical analyzer and an electrochemical workstation, and the current value obtained by the electrochemical workstation test was linearly fitted to evaluate its linearity, and the test results are shown in Table 4.

[0044] Table 4 Linear relationship table of triglyceride quality control solution

[0045] As can be seen from Table 4, the sensitivity and linearity of the triglyceride quality control solutions provided by Examples 5 to 9 are significantly higher than those of Comparative Examples 1 and 2, and the sensitivity and linearity of Examples 5 to 9 are similar; this is because a dispersant and a lipoprotein lipase activator are added to the base solution of Example 5 prepared by the present invention, and the dispersant enables the triglyceride concentrate to be evenly dispersed in the base solution, so that the dispersed system remains in a stable state; the lipoprotein lipase activator enables the lipase in the triglyceride test paper to maintain a high catalytic efficiency; through the synergistic effect of the dispersant and the lipoprotein lipase activator, the hydrolysis of triglycerides is jointly promoted, so that the triglyceride quality control solution provided by the present invention has a good sensitivity and linear relationship.

[0046] The stability of the triglyceride lipid control solution provided by the present invention was evaluated: The triglyceride quality control solutions of four concentration levels in Example 5 and Comparative Examples 1 and 2 were divided into vials with a volume of 5 mL each, sealed and placed in thermostats at 2°C-8°C and 60°C. One bottle of triglyceride quality control solution of different concentration levels was taken from the thermostats at 2°C-8°C and 60°C every other week, and all were tested using Mindray BS-360s biochemical analyzer. The test kit was a triglyceride determination kit produced by Mindray. After the test, the deviation of the biochemical instrument test value of the triglyceride quality control solution placed under 60°C conditions and the triglyceride quality control solution placed under 2°C-8°C conditions was calculated for each week, and the test was continued for 10 weeks. The triglyceride concentration detected by the 60°C accelerated aging sample and the triglyceride concentration detected by the 2°C-8°C sample were compared, and the relative deviation of the triglyceride concentration detected by the 60°C accelerated aging sample and the triglyceride concentration detected by the 2°C-8°C sample was calculated based on the mean biochemical concentration of triglyceride detected by the sample at 2-8°C. The test results are shown in Tables 5-7.

[0047] Table 5 Accelerated stability test results of Example 5

[0048] Table 6 Accelerated stability test results of Comparative Example 1

[0049] Table 7 Accelerated stability test results of Comparative Example 2

[0050] As can be seen from Tables 5-7, after the triglyceride quality control solution provided in Example 5 was placed at 60°C for 10 weeks, the deviation was within ±4% compared with the triglyceride quality control solution placed at 2°C-8°C; while the deviations of Comparative Examples 1 and 2 exceeded ±10% under the same conditions. This indicates that the triglyceride quality control solution prepared by the present invention has good stability.

[0051] Matters not covered by the present invention are known technologies.

[0052] The technical features of the above embodiments may be combined arbitrarily. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0053] The above-described embodiments only express several implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention. It should be pointed out that, for a person of ordinary skill in the art, several modifications and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the protection scope of the present application shall be subject to the attached claims.

[0054] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A triglyceride quality control solution, characterized in that: It comprises a triglyceride concentrate and a base liquid containing a dispersant and an activator, wherein the triglyceride concentrate is added into the base liquid and dispersed homogeneously; The activator is a lipoprotein lipase activator; The volume ratio of the triglyceride concentrate to the base liquid is 1:(10-300).

2. The triglyceride quality control solution according to claim 1, characterized in that: The matrix of the triglyceride concentrate is bovine serum, and the concentration is 6000 mg / -8000 mg / dL.

3. The triglyceride quality control solution according to claim 1, characterized in that: The concentration of the activator is 2g / L-10g / L.

4. The triglyceride quality control solution according to claim 1, characterized in that The concentration of the dispersant is 1 g / L-6 g / L.

5. The triglyceride quality control solution according to claim 1, characterized in that: The base solution also includes a buffer, a stabilizer, and a preservative.

6. The triglyceride quality control solution according to claim 1, characterized in that: The activator includes one or more of ibromide, N-2-nitro-4-chlorophenyl-4-[(phosphonic acid diethyl ester) methyl] benzohydrazide, and N-2-tetrazolyl-4-chlorophenyl-4-[(phosphonic acid diethyl ester) methyl] benzamide.

7. The triglyceride quality control solution according to claim 1, characterized in that: The dispersant includes one or more of triammonium citrate, ammonium polyacrylate, and sodium tripolyphosphate.

8. The triglyceride quality control solution according to claim 5, characterized in that: The buffer solution is a buffer solution with a pH of 7, including one or more of PBS buffer solution, Mops buffer solution, HEPES buffer solution, TES buffer solution, and PIPES-sesquisodium salt, and the concentration of the buffer solution is 0.01 mol / L-0.2 mol / L.

9. The triglyceride quality control solution according to claim 5, characterized in that: The stabilizer includes one or more of sucrose, trehalose, dextran and lactose, and the concentration of the stabilizer is 5g / L-20g / L.

10. The triglyceride quality control solution according to claim 5, characterized in that: The preservative includes one or more of potassium sorbate, sodium azide, and Proclin 300, and the concentration of the preservative is 0.3 g / L-1 g / L.

11. The triglyceride quality control solution according to claim 1, characterized in that: In the triglyceride quality control solution, the triglyceride concentration is 0.5mmol / -8.0mmol / L.

12. A method for preparing a triglyceride quality control solution, characterized in that: The method for preparing the triglyceride quality control solution according to any one of claims 1 to 11 comprises the following steps: Prepare the base liquid raw materials and triglyceride concentrate according to the proportion; Mixing base liquid raw materials to prepare base liquid; The triglyceride concentrate is added to the base solution, and the triglyceride quality control solution is obtained after homogenization and dispersion.

13. The method for preparing a triglyceride quality control solution according to claim 12, characterized in that: The following steps are involved: Prepare the base liquid raw materials and triglyceride concentrate according to the proportion; An activator, a dispersant, a stabilizer and a preservative are sequentially added into a buffer solution to prepare a base solution; Add triglyceride concentrate to the base solution and homogenize and disperse at 25°C for 5 minutes to obtain the triglyceride quality control solution.

14. The method for preparing a triglyceride quality control solution according to claim 13, wherein: The speed of the homogenizing and dispersing is 150 rpm-1440 rpm; The homogenous dispersion is performed using a non-intrusive material homogenizer.

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