Method for reducing organic solvent concentration and flammable and explosive risks in podophyllotoxin drying process

Abstract

The invention is applicable to the technical field of podophyllotoxin, and provides a method for reducing organic solvent concentration and flammable and explosive risks in a podophyllotoxin drying process, the specific operation steps are as follows: S1, sample selection: selecting clean podophyllum hexandrum, umbrellaleaf, podophyllum zingiberensis and podophyllum serratum, sampling roots or seeds of all sample plants, and grinding into fine powder; s2, extracting samples: taking different types of powder samples, putting the powder samples into containers with sample names, adding the same proper amount of ethanol into each sample container, oscillating and extracting in a water bath at 40 DEG C for 30 minutes, centrifuging for 10 minutes, and taking supernate for later use; according to the method for reducing the concentration of the organic solvent and the flammable and explosive risks in the podophyllotoxin drying process, the podophyllotoxin crystals are gradually heated and dried, and the situation that the podophyllotoxin crystals are flammable and explosive due to the fact that the temperature of the podophyllotoxin crystals is too fast is reduced to a certain extent.

Description

Technical Field

[0001] The present invention relates to the technical field of podophyllotoxin, and more particularly to a method for reducing the concentration of organic solvents and the risk of flammability and explosion during the drying process of podophyllotoxin. Background Art

[0002] Podophyllotoxin is a substance isolated from podophyllum plants with anticancer and insecticidal activity. It is widely used in the treatment of malignant tumors, skin proliferative diseases and the development of botanical insecticides. Foreign researchers have extracted and isolated podophyllotoxin from Podophyllum peltata in America and Podophyllum in India. In China, Podophyllum peltata, Podophyllum peltata and Podophyllum occidentalis are commonly used plant resources for extracting podophyllotoxin.

[0003] At present, some existing extraction and separation processes of podophyllotoxin include CO2 supercritical extraction, methanol reflux-water-chloroform purification and solvent crystallization-chromatography. However, some existing podophyllotoxins are directly heated up rapidly when drying. Since podophyllotoxin contains some organic solvents, when podophyllotoxin is heated up rapidly, the organic solvents are easily vaporized quickly, causing the organic solvent to become flammable and explosive. Summary of the invention

[0004] In view of the shortcomings of the prior art, the object of the present invention is to provide a method for reducing the organic solvent concentration and the flammability and explosion risk during the drying process of podophyllotoxin.

[0005] To achieve the above object, the present invention provides the following technical solutions: A method for reducing the concentration of organic solvents and the risk of flammability and explosion during the drying process of podophyllotoxin, wherein the specific operating steps are as follows: S1. Sample selection: Select clean Psoralea corylifolia, Polygonum multiflorum, Podophyllum peltata and Podophyllum sphaerocephalum, and sample the roots or seeds of all sample plants and grind them into fine powder; S2. Extract samples: Take different types of powder samples, put them into containers with the sample names, add the same amount of ethanol into each sample container, shake and extract in a 40°C water bath for 30 minutes, centrifuge for 10 minutes, and take the supernatant for later use; S3, the combined extracts were concentrated to obtain an extract, and the extract was ultrasonically extracted twice with 3-4 times the amount of ethyl acetate, each extraction time was 1.5 hours, and the filtrate was concentrated to 1 / 6 of the original volume, allowed to stand for crystallization, and filtered to obtain a crude product; S4, dissolve the above crude product in 300 ml of anhydrous methanol, add 3 g of activated carbon, reflux for 1 h, filter, add 2 L of chloroform to the filtrate, centrifuge, concentrate the organic layer, and allow to crystallize; S5, add 200-350ml of anhydrous methanol to the crystals for reflux dissolution, concentrate, cool and crystallize, repeat 3 times, filter out the crystals, and dry to obtain podophyllotoxin; S6. Detection: prepare a TCL plate, evenly apply a silica gel layer on the TCL plate, take an appropriate amount of supernatant, and evenly sprinkle it on the TCL plate with a capillary; S7, Development: Place the TCL plate vertically in the chromatographic tank, add an appropriate amount of mobile phase solution into the tank until the liquid level exceeds the height of the silica gel layer; S8. Seal the chromatographic tank: Seal with appropriate materials to prevent solvent volatilization; S9. Carry out chromatography at a suitable temperature, so that the mobile phase gradually rises and penetrates into the silica gel layer. At a suitable position, observe the spot on the chromatographic plate and take it out quickly; S10. Determination: Soak the chromatographic plate in a color developer to develop spots, and determine the content of podophyllotoxin by measuring the size and color of the spots.

[0006] The present invention is further configured as follows: the sample in S1 is crushed to 20 meshes, 0.5-2 kg is taken and put into a flask, and 4-7 times the amount of 85% methanol solution is added.

[0007] The present invention is further configured as follows: ultrasonic extraction is performed three times in S2, each extraction lasting 1 hour.

[0008] The present invention is further configured as follows: filtering and combining the filtrate in S5, then reducing pressure and concentrating to recover methanol to obtain an alcohol extract, taking 20.0 g of the sample and putting it into a methanol solution for ultrasonic dissolution, using a dextran gel column chromatography for separation, using methanol for elution and combining 8 to 15 fractions, reducing pressure and recovering the solvent to dryness, and obtaining an extract mainly containing lignan components.

[0009] The present invention is further configured as follows: the podophyllotoxin crystals in S5 are first heated to 70 degrees and heated for 10 minutes; then heated to 80 degrees and heated for 20 minutes; then heated to 90 degrees and heated for 20 minutes; then heated to 100 degrees and heated for 20 minutes; and then heated to 110 degrees and heated for 20 minutes.

[0010] The present invention is further configured as follows: the heated crystals are finally heated to 120 degrees, and dry-heated for 3-4 hours to complete the drying.

[0011] The present invention is further configured as follows: the diameter of the dextran gel column chromatography column is 4.5 cm, and the column height is 180 cm.

[0012] The present invention is further configured as follows: in S10, the concentration of podophyllotoxin is calculated by a standard curve method.

[0013] The advantages of the present invention are: 1. The present invention discloses a method for reducing the concentration of organic solvents and the risk of flammability and explosion during the drying process of podophyllotoxin. By gradually heating and drying the podophyllotoxin crystals, the temperature rise of the podophyllotoxin crystals is reduced to a certain extent, making the podophyllotoxin crystals flammable and explosive, thereby making the extraction of podophyllotoxin crystals safer.

[0014] 2. The present invention discloses a method for reducing the concentration of organic solvents and the risk of flammability and explosion during the drying process of podophyllotoxin. The method removes the toxic organic solvent residues in podophyllotoxin by gradually increasing the temperature to remove the solvent, thereby ensuring the purity and quality of podophyllotoxin. The method is particularly suitable for industrial mass production.

[0015] 3. The present invention provides a method for reducing the organic solvent concentration and flammability and explosion risk in the drying process of podophyllotoxin. According to experimental operation, obvious spots can be observed on the TCL plate. The content of podophyllotoxin can be inferred according to the size and color of the spots. By calculating and comparing the results of the standard curve, an accurate content determination result can be obtained. DETAILED DESCRIPTION

[0016] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application may be combined with each other.

[0017] It should be noted that, unless otherwise specified, all technical and scientific terms used in this application have the same meanings as commonly understood by ordinary technicians in the technical field to which this application belongs.

[0018] The present invention provides the following technical solution: a method for reducing the concentration of organic solvents and the risk of flammability and explosion during the drying process of podophyllotoxin, and the specific operating steps are as follows: S1. Sample selection: Select clean Psoralea corylifolia, Polygonum multiflorum, Podophyllum peltata and Podophyllum sphaerocephalum, and sample the roots or seeds of all sample plants and grind them into fine powder; S2. Extract samples: Take different types of powder samples, put them into containers with the sample names, add the same amount of ethanol into each sample container, shake and extract in a 40°C water bath for 30 minutes, centrifuge for 10 minutes, and take the supernatant for later use; Further: the sample in S1 is crushed into 20 meshes, 0.5 kg is taken and put into a flask, and 4 times the amount of 85% methanol solution is added.

[0019] Furthermore, ultrasonic extraction was performed three times, each time for 1 hour; S3, the combined extracts were concentrated to obtain an extract, and the extract was ultrasonically extracted twice with 3 times the amount of ethyl acetate, each extraction time was 1.5 hours, and the filtrate was concentrated to 1 / 6 of the original volume, allowed to stand for crystallization, and filtered to obtain a crude product; S4, dissolve the above crude product in 300 ml of anhydrous methanol, add 3 g of activated carbon, reflux for 1 h, filter, add 2 L of chloroform to the filtrate, centrifuge, concentrate the organic layer, and allow to crystallize; S5, add 200 ml of anhydrous methanol to the crystals and reflux to dissolve, concentrate, cool and crystallize, repeat 3 times, filter out the crystals, and dry to obtain podophyllotoxin; Furthermore, the filtrate is filtered and combined, and then the methanol is concentrated under reduced pressure to obtain an alcohol extract, 20.0 g of the sample is put into a methanol solution for ultrasonic dissolution, and separated by dextran gel column chromatography (column diameter: 4.5 cm, column height: 180 cm), 8 to 15 fractions are combined by methanol elution, and the solvent is recovered under reduced pressure to dryness to obtain an extract mainly containing lignan components.

[0020] S6. Detection: prepare a TCL plate, evenly apply a silica gel layer on the TCL plate, take an appropriate amount of supernatant, and evenly sprinkle it on the TCL plate with a capillary; S7, Development: Place the TCL plate vertically in the chromatographic tank, add an appropriate amount of mobile phase solution into the tank until the liquid level exceeds the height of the silica gel layer; S8. Seal the chromatographic tank: Seal with appropriate materials to prevent solvent volatilization; S9. Carry out chromatography at a suitable temperature, so that the mobile phase gradually rises and penetrates into the silica gel layer. At a suitable position, observe the spot on the chromatographic plate and take it out quickly; S10. Determination: Soak the chromatographic plate in a color developer to develop spots, and determine the content of podophyllotoxin by measuring the size and color of the spots. Calculate the concentration of podophyllotoxin by a standard curve method.

[0021] According to the experimental operation, obvious spots can be observed on the TCL plate. The content of podophyllotoxin can be inferred based on the size and color of the spots. By calculating and comparing the results of the standard curve, accurate content determination results can be obtained.

[0022] Embodiment 2: Based on Embodiment 1: S1. Sample selection: Select clean Psoralea corylifolia, Polygonum multiflorum, Podophyllum peltata and Podophyllum sphaerocephalum, and sample the roots or seeds of all sample plants and grind them into fine powder; S2. Extract samples: Take different types of powder samples, put them into containers with the sample names, add the same amount of ethanol into each sample container, shake and extract in a 40°C water bath for 30 minutes, centrifuge for 10 minutes, and take the supernatant for later use; Further: the sample in S1 is crushed into 20 meshes, 1 kg is taken and put into a flask, and 5 times the amount of 85% methanol solution is added.

[0023] Furthermore, ultrasonic extraction was performed three times, each time for 1 hour; S3, the combined extracts were concentrated to obtain an extract, and the extract was ultrasonically extracted twice with 3.5 times the amount of ethyl acetate, each extraction time was 1.5 hours, and the filtrate was concentrated to 1 / 6 of the original volume, allowed to stand for crystallization, and filtered to obtain a crude product; S4, dissolve the above crude product in 300 ml of anhydrous methanol, add 3 g of activated carbon, reflux for 1 h, filter, add 2 L of chloroform to the filtrate, centrifuge, concentrate the organic layer, and allow to crystallize; S5, add 300 ml of anhydrous methanol to the crystals and reflux to dissolve, concentrate, cool and crystallize, repeat 3 times, filter out the crystals, and dry to obtain podophyllotoxin; Furthermore, the filtrate is filtered and combined, and then the methanol is concentrated under reduced pressure to obtain an alcohol extract, 20.0 g of the sample is put into a methanol solution for ultrasonic dissolution, and separated by dextran gel column chromatography (column diameter: 4.5 cm, column height: 180 cm), 8 to 15 fractions are combined by methanol elution, and the solvent is recovered under reduced pressure to dryness to obtain an extract mainly containing lignan components.

[0024] Furthermore, the podophyllotoxin crystals are first heated to 70 degrees and heated for 10 minutes; then heated to 80 degrees and heated for 20 minutes; then heated to 90 degrees and heated for 20 minutes; then heated to 100 degrees and heated for 20 minutes; then continued to heat to 110 degrees and heated for 20 minutes; finally heated to 120 degrees and dried and heated for 3.5 hours to complete drying.

[0025] By gradually heating and drying the podophyllotoxin crystals, the rapid heating of the podophyllotoxin crystals can be reduced to a certain extent, which would make the podophyllotoxin crystals flammable and explosive, thereby making the extraction of the podophyllotoxin crystals safer.

[0026] Embodiment 3: Based on Embodiments 1 and 2: S1. Sample selection: Select clean Psoralea corylifolia, Polygonum multiflorum, Podophyllum peltata and Podophyllum sphaerocephalum, and sample the roots or seeds of all sample plants and grind them into fine powder; S2. Extract samples: Take different types of powder samples, put them into containers with the sample names, add the same amount of ethanol into each sample container, shake and extract in a 40°C water bath for 30 minutes, centrifuge for 10 minutes, and take the supernatant for later use; Further: the sample in S1 is crushed into 20 meshes, 0.5-2 kg is taken into a flask, and 4-7 times the amount of 85% methanol solution is added.

[0027] Furthermore, ultrasonic extraction was performed three times, each time for 1 hour; S3, the combined extracts were concentrated to obtain an extract, and the extract was ultrasonically extracted twice with 4 times the amount of ethyl acetate, each extraction time was 1.5 hours, and the filtrate was concentrated to 1 / 6 of the original volume, allowed to stand for crystallization, and filtered to obtain a crude product; S4, dissolve the above crude product in 300 ml of anhydrous methanol, add 3 g of activated carbon, reflux for 1 h, filter, add 2 L of chloroform to the filtrate, centrifuge, concentrate the organic layer, and allow to crystallize; S5, add 350 ml of anhydrous methanol to the crystals and reflux to dissolve, concentrate, cool and crystallize, repeat 3 times, filter out the crystals, and dry to obtain podophyllotoxin; Furthermore, the filtrate is filtered and combined, and then the methanol is concentrated under reduced pressure to obtain an alcohol extract, 20.0 g of the sample is put into a methanol solution for ultrasonic dissolution, and separated by dextran gel column chromatography (column diameter: 4.5 cm, column height: 180 cm), 8 to 15 fractions are combined by methanol elution, and the solvent is recovered under reduced pressure to dryness to obtain an extract mainly containing lignan components.

[0028] Furthermore, the podophyllotoxin crystals are first heated to 70 degrees and heated for 10 minutes; then heated to 80 degrees and heated for 20 minutes; then heated to 90 degrees and heated for 20 minutes; then heated to 100 degrees and heated for 20 minutes; then heated to 110 degrees and heated for 20 minutes; finally heated to 120 degrees and dried and heated for 3-4 hours to complete drying.

[0029] By gradually heating and drying the podophyllotoxin crystals, the rapid heating of the podophyllotoxin crystals can be reduced to a certain extent, which would make the podophyllotoxin crystals flammable and explosive, thereby making the extraction of the podophyllotoxin crystals safer.

[0030] S6. Detection: prepare a TCL plate, evenly apply a silica gel layer on the TCL plate, take an appropriate amount of supernatant, and evenly sprinkle it on the TCL plate with a capillary; S7, Development: Place the TCL plate vertically in the chromatographic tank, add an appropriate amount of mobile phase solution into the tank until the liquid level exceeds the height of the silica gel layer; S8. Seal the chromatographic tank: Seal with appropriate materials to prevent solvent volatilization; S9. Carry out chromatography at a suitable temperature, so that the mobile phase gradually rises and penetrates into the silica gel layer. At a suitable position, observe the spot on the chromatographic plate and take it out quickly; S10. Determination: Soak the chromatographic plate in a color developer to develop spots, and determine the content of podophyllotoxin by measuring the size and color of the spots. Calculate the concentration of podophyllotoxin by a standard curve method.

[0031] According to the experimental operation, obvious spots can be observed on the TCL plate. The content of podophyllotoxin can be inferred based on the size and color of the spots. By calculating and comparing the results of the standard curve, accurate content determination results can be obtained.

[0032] Embodiment 4: Based on Embodiments 1, 2 and 3: S1. Sample selection: Select clean Psoralea corylifolia, Polygonum multiflorum, Podophyllum peltata and Podophyllum sphaerocephalum, and sample the roots or seeds of all sample plants and grind them into fine powder; S2. Extract samples: Take different types of powder samples, put them into containers with the sample names, add the same amount of ethanol into each sample container, shake and extract in a 40°C water bath for 30 minutes, centrifuge for 10 minutes, and take the supernatant for later use; Further: the sample in S1 is crushed into 20 meshes, 0.5-2 kg is taken into a flask, and 4-7 times the amount of 85% methanol solution is added.

[0033] Furthermore, ultrasonic extraction was performed three times, each time for 1 hour; S3, the combined extracts were concentrated to obtain an extract, and the extract was ultrasonically extracted twice with 4 times the amount of ethyl acetate, each extraction time was 1.5 hours, and the filtrate was concentrated to 1 / 6 of the original volume, allowed to stand for crystallization, and filtered to obtain a crude product; S4, dissolve the above crude product in 300 ml of anhydrous methanol, add 3 g of activated carbon, reflux for 1 h, filter, add 2 L of chloroform to the filtrate, centrifuge, concentrate the organic layer, and allow to crystallize; S5, add 350 ml of anhydrous methanol to the crystals and reflux to dissolve, concentrate, cool and crystallize, repeat 3 times, filter out the crystals, and dry to obtain podophyllotoxin; Furthermore, the filtrate is filtered and combined, and then the methanol is concentrated under reduced pressure to obtain an alcohol extract, 20.0 g of the sample is put into a methanol solution for ultrasonic dissolution, and separated by dextran gel column chromatography (column diameter: 4.5 cm, column height: 180 cm), 8 to 15 fractions are combined by methanol elution, and the solvent is recovered under reduced pressure to dryness to obtain an extract mainly containing lignan components.

[0034] Furthermore, the podophyllotoxin crystals are first heated to 70 degrees and heated for 10 minutes; then heated to 80 degrees and heated for 20 minutes; then heated to 90 degrees and heated for 20 minutes; then heated to 100 degrees and heated for 20 minutes; then heated to 110 degrees and heated for 20 minutes; finally heated to 120 degrees and dried and heated for 4 hours to complete the drying.

[0035] By gradually heating and drying the podophyllotoxin crystals, the rapid heating of the podophyllotoxin crystals can be reduced to a certain extent, which would make the podophyllotoxin crystals flammable and explosive, thereby making the extraction of the podophyllotoxin crystals safer.

[0036] S6. Detection: prepare a TCL plate, evenly apply a silica gel layer on the TCL plate, take an appropriate amount of supernatant, and evenly sprinkle it on the TCL plate with a capillary; S7, Development: Place the TCL plate vertically in the chromatographic tank, add an appropriate amount of mobile phase solution into the tank until the liquid level exceeds the height of the silica gel layer; S8. Seal the chromatographic tank: Seal with appropriate materials to prevent solvent volatilization; S9. Carry out chromatography at a suitable temperature, so that the mobile phase gradually rises and penetrates into the silica gel layer. At a suitable position, observe the spot on the chromatographic plate and take it out quickly; S10. Determination: Soak the chromatographic plate in a color developer to develop spots, and determine the content of podophyllotoxin by measuring the size and color of the spots. Calculate the concentration of podophyllotoxin by a standard curve method.

[0037] According to the experimental operation, obvious spots can be observed on the TCL plate. The content of podophyllotoxin can be inferred based on the size and color of the spots. By calculating and comparing the results of the standard curve, accurate content determination results can be obtained.

[0038] Obviously, the above-described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0039] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0040] It should be noted that the terms "first", "second", etc. in the specification and claims of the present application are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the numbers used in this way can be interchanged where appropriate, so that the embodiments of the present application described herein can be implemented in an order other than those illustrated or described herein.

[0041] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for reducing the organic solvent concentration and flammability and explosion risk during the drying process of podophyllotoxin, characterized in that: The specific operation steps are as follows: S1. Sample selection: Select clean Psoralea corylifolia, Polygonum multiflorum, Podophyllum peltolacca and Podophyllum sphaerocephalum, and sample the roots or seeds of all sample plants and grind them into fine powder; S2. Extract samples: Take different types of powder samples, put them into containers with the sample names, add the same amount of ethanol into each sample container, shake and extract in a 40°C water bath for 30 minutes, centrifuge for 10 minutes, and take the supernatant for later use; S3, the combined extracts were concentrated to obtain an extract, and the extract was ultrasonically extracted twice with 3-4 times the amount of ethyl acetate, each extraction time was 1.5 hours, and the filtrate was concentrated to 1 / 6 of the original volume, allowed to stand for crystallization, and filtered to obtain a crude product; S4, dissolve the above crude product in 300 ml of anhydrous methanol, add 3 g of activated carbon, reflux for 1 h, filter, add 2 L of chloroform to the filtrate, centrifuge, concentrate the organic layer, and allow to crystallize; S5, add 200-350ml of anhydrous methanol to the crystals for reflux dissolution, concentrate, cool and crystallize, repeat 3 times, filter out the crystals, and dry to obtain podophyllotoxin; S6. Detection: prepare a TCL plate, evenly apply a silica gel layer on the TCL plate, take an appropriate amount of supernatant, and evenly sprinkle it on the TCL plate with a capillary; S7, Development: Place the TCL plate vertically in the chromatographic tank, add an appropriate amount of mobile phase solution into the tank until the liquid level exceeds the height of the silica gel layer; S8. Seal the chromatographic tank: Seal with appropriate materials to prevent solvent volatilization; S9. Carry out chromatography at a suitable temperature, so that the mobile phase gradually rises and penetrates into the silica gel layer. At a suitable position, observe the spot on the chromatographic plate and take it out quickly; S10. Determination: Soak the chromatographic plate in a color developer to develop spots, and determine the content of podophyllotoxin by measuring the size and color of the spots.

2. A method for reducing the organic solvent concentration and flammability and explosion risk during the drying process of podophyllotoxin according to claim 1, characterized in that: The sample in S1 is crushed into 20 meshes, 0.5-2 kg is taken and put into a flask, and 4-7 times the amount of 85% methanol solution is added.

3. A method for reducing the organic solvent concentration and flammability and explosion risk during the drying process of podophyllotoxin according to claim 2, characterized in that: Ultrasonic extraction was performed three times in S2, each time for 1 hour.

4. A method for reducing the organic solvent concentration and flammability and explosion risk during the drying process of podophyllotoxin according to claim 3, characterized in that: The filtrate in S5 is filtered and combined, and then concentrated under reduced pressure to recover methanol to obtain an alcohol extract, 20.0 g of the sample is put into a methanol solution for ultrasonic dissolution, separated by dextran gel column chromatography, eluted with methanol and 8 to 15 fractions are combined, the solvent is recovered under reduced pressure to dryness, and an extract mainly containing lignan components is obtained.

5. A method for reducing the concentration of organic solvents and the risk of flammability and explosion during the drying process of podophyllotoxin according to claim 4, characterized in that: The podophyllotoxin crystals in S5 are first heated to 70 degrees and heated for 10 minutes; then heated to 80 degrees and heated for 20 minutes; then heated to 90 degrees and heated for 20 minutes; then heated to 100 degrees and heated for 20 minutes; and then heated to 110 degrees and heated for 20 minutes.

6. A method for reducing the organic solvent concentration and flammability and explosion risk during the podophyllotoxin drying process according to claim 5, characterized in that: The heated crystals are finally heated to 120 degrees and dried for 3-4 hours to complete the drying.

7. The method for reducing the organic solvent concentration and the flammability and explosion risk during the drying process of podophyllotoxin according to claim 4, characterized in that: The diameter of the dextran gel column chromatography column is 4.5 cm, and the column height is 180 cm.

8. The method for reducing the organic solvent concentration and flammability and explosion risk during the podophyllotoxin drying process according to claim 1, characterized in that: In S10, the concentration of podophyllotoxin is calculated by the standard curve method.