A food safety detection work station and a control system and control method thereof

Abstract

The application relates to the technical field of food safety detection, in particular to a food safety detection workstation and a control system and control method thereof, which comprise a detection table, a pretreatment module and a detection module arranged on the detection table; the pretreatment module is used for performing various pretreatment operations on a to-be-detected sample according to a required process, and provides a reaction solution of the pretreated to-be-detected sample for the detection module; the detection module comprises a third detection module, the third detection module comprises a reagent placing rack, a microwell strip placing channel, a reagent liquid adding module, a plate washing mechanism, a suction tip box mechanism, a desorption tip mechanism, a reading module and a third mechanical arm; the application can continuously sample without interruption, process multiple samples at the same time, improve the detection efficiency, shorten the detection period and reduce the test error.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of food safety detection, and in particular to a food safety detection workstation, a control system thereof and a control method. BACKGROUND

[0002] With the increasing requirements of consumers on food quality and the increasing food safety incidents, the public pays more and more attention to food safety, and the monitoring of food quality and safety is also paid more and more attention.

[0003] The existing several food safety detection devices, some small and portable devices capable of on-site detection, often need manual operation and cannot realize automatic detection, and due to on-site detection, are greatly affected by the environment and are difficult to avoid sample pollution problems. Some detection devices capable of automatic experimental operation usually have preset processes for rapid detection projects, and can only complete the operation of a single test project at the same time. The existing food safety detection devices are usually fluorescence detection card-based analysis instruments, which usually need manual operation to load the detection card, increasing the risk of human error, having low detection flux, and thus low detection efficiency and large experimental error.

[0004] Therefore, there is an urgent need for a food safety detection workstation, a control system thereof and a control method, which can continuously sample without interruption, process multiple samples at the same time, improve detection efficiency, shorten detection period and reduce experimental error. SUMMARY

[0005] In order to solve the above technical problems, the present application provides a food safety detection workstation, a control system thereof and a control method, which can continuously sample without interruption, process multiple samples at the same time, improve detection efficiency, shorten detection period and reduce experimental error.

[0006] The present application provides a food safety detection workstation, comprising:

[0007] a detection table, and a pretreatment module and a detection module arranged on the detection table;

[0008] The pretreatment module is used for pretreating the sample to obtain a to-be-detected solution for detection by the detection module.

[0009] The detection module comprises a third detection module, the third detection module comprises a reagent placing rack, a micro-hole strip placing channel, a reagent adding module, a plate washing mechanism, a suction tip box mechanism, a tip removing mechanism, a reading module and a third mechanical arm; the micro-hole strip placing channel is used for placing enzyme-labeled micro-holes, the reagent placing rack is arranged above the micro-hole strip placing channel and is used for placing reagents required for detection, the reagent adding module is arranged between the micro-hole strip placing channel and the reagent placing rack and is used for adding reagents to the enzyme-labeled micro-holes to react with the to-be-detected solution, the plate washing mechanism is arranged at one end of the micro-hole strip placing channel close to the edge of the detection table and is used for washing the enzyme-labeled micro-holes, the suction tip box mechanism and the tip removing mechanism are arranged above the micro-hole strip placing channel and are arranged side by side on one side of the reagent placing rack, the suction tip box mechanism is used for storing suction tips to replace the needle of the liquid needle on the third mechanical arm, the tip removing mechanism is used for removing the used needle, the reading module is arranged between the micro-hole strip placing channel and the reagent placing rack and is used for measuring the result of the to-be-detected solution after reaction in the enzyme-labeled micro-holes, and the third mechanical arm is used for sucking the lower liquid in the centrifugal tube containing the reaction liquid from the pre-processing module into the enzyme-labeled micro-holes or sucking the reagent in the reagent placing rack into the enzyme-labeled micro-holes.

[0010] Further, the pre-processing module comprises a first processing module and a second processing module;

[0011] The first processing module comprises a sample loading channel, a front liquid adding module, a sample transfer mechanism, a first oscillator, a first centrifuge and a first mechanical arm; the sample loading channel is used for placing sample tubes, the sample transfer mechanism is used for moving the sample tubes in the sample loading channel to the front liquid adding module or the second processing module, the front liquid adding module is arranged on one side of the sample transfer mechanism to enable the sample tubes supported by the sample transfer mechanism to be moved to the corresponding needle positions of the front liquid adding module, and the first mechanical arm is used for transferring the sample tubes to the first oscillator, the first centrifuge or the sample transfer mechanism;

[0012] The second processing module comprises a waiting turntable and a second mechanical arm; the waiting turntable is used for placing centrifugal tubes, and the second mechanical arm is used for sucking supernatant from the sample tubes in the sample transfer mechanism into the centrifugal tubes and transferring the centrifugal tubes to the waiting turntable.

[0013] Further, the first processing module further comprises an incubation system, and the first mechanical arm is further used for transferring the sample tubes to the incubation system;

[0014] The second processing module further comprises a second centrifuge, a second centrifuge balancing mechanism, a second oscillator, an air blowing system, a post-liquid adding module and a liquid needle cleaning mechanism; the second centrifuge balancing mechanism is used for balancing operation of the second centrifuge; the air blowing system is used for concentrate operation of sample pretreatment extract; the post-liquid adding module is used for liquid adding operation of centrifuge tubes placed on a rotating disc of the post-liquid adding module; the liquid needle cleaning mechanism is arranged at an intermediate position between the end of the sample transferring mechanism and the air blowing system, and is used for cleaning a liquid needle arranged on the second mechanical arm; and the second mechanical arm is further used for transferring centrifuge tubes into the second centrifuge, the second oscillator, the air blowing system or the post-liquid adding module.

[0015] Further, the first mechanical arm is in a U-shaped structure, comprising two longitudinal beams, a cross beam and a clamping jaw, the two longitudinal beams are each provided with a sliding rail, the two ends of the cross beam are fixed on the sliding rails of the two longitudinal beams, the cross beam is provided with a sliding rail, and the clamping jaw is fixed on the sliding rail of the cross beam; the cross beam can slide back and forth along the longitudinal beams through the sliding rails of the longitudinal beams; and the clamping jaw can slide left and right along the cross beam through the sliding rail of the cross beam.

[0016] The second mechanical arm is in an L-shaped structure, comprising a longitudinal beam, a cross beam, a clamping jaw and a liquid needle; the cross beam is provided with a sliding rail; one end of the longitudinal beam is fixed on the sliding rail of the cross beam; the lower side of the longitudinal beam is a sliding rail; the clamping jaw and the liquid needle are fixed on the sliding rail of the longitudinal beam; the liquid needle is arranged on the side close to the cross beam; the clamping jaw is arranged on the side away from the cross beam; the longitudinal beam can slide left and right along the cross beam through the sliding rail of the cross beam; and the clamping jaw and the liquid needle can slide back and forth along the longitudinal beam through the sliding rail of the longitudinal beam.

[0017] The third mechanical arm is in an L-shaped structure, comprising a longitudinal beam, a cross beam and a liquid needle; the longitudinal beam is provided with a sliding rail; one end of the cross beam is fixed on the sliding rail of the longitudinal beam; the lower side of the cross beam is a sliding rail; and the liquid needle is fixed on the sliding rail of the cross beam; the cross beam can slide back and forth along the longitudinal beam through the sliding rail of the longitudinal beam; and the liquid needle can slide left and right along the cross beam through the sliding rail of the cross beam.

[0018] Further, the liquid needle of the second mechanical arm is further provided with a liquid level detection system, which is used for detecting the liquid level height in the sample tube, so that the liquid needle and the liquid level are always in the minimum effective contact distance.

[0019] Further, the air blowing system comprises two air blowing modules; each air blowing module comprises six air blowing positions and six air blowing waiting positions; the air blowing positions and the air blowing waiting positions are arranged in two rows on a rotating disc of the air blowing module; and the air blowing positions and the air blowing waiting positions are switched in position by rotating the rotating disc.

[0020] Further, the blowing pipe of the air blowing module is segmentedly lowered according to parameter setting, and always maintains the minimum effective air blowing distance with the liquid level in the sample tube.

[0021] The application also provides a control system of a food safety detection workstation, which is realized based on the food safety detection workstation.

[0022] The data analysis module is used for creating a test item before the experiment starts, and creating a standard curve of the test item by scanning a standard curve two-dimensional code on a corresponding item kit or performing a standard curve experiment; and is also used for generating a data analysis report according to an experimental result after the experiment ends, and providing a printing report.

[0023] The experiment running module is used for performing test setting, including performing a standard curve experiment, placing standard curve preparation reagents at corresponding positions on the food safety detection workstation according to a system prompt, after the reagents are placed, the equipment automatically enters a corresponding product standard curve preparation, acquires standard curve data of the item, after the standard curve data is received, the system automatically saves the standard curve data with a corresponding name; and is also used for performing a sample detection experiment, first performing sample code scanning and sample loading, setting a number of sample tubes in a set of loading racks and a preset test process to be performed, and when the sample loading is completed, the equipment automatically performs a detection process.

[0024] Further, the control system further comprises:

[0025] The pipette tip setting module is used for setting a starting use position of a pipette tip before the experiment starts.

[0026] The liquid path maintenance module is used for performing liquid discharge work on a front liquid adding liquid path, a rear liquid adding liquid path, AB liquid and cleaning liquid with reagents before the experiment starts, and discharging waste liquid in corresponding reagent bottles into a liquid discharge groove.

[0027] The process detection module is used for performing real-time tracking on conditions of each module of the equipment in the experiment process.

[0028] The equipment pause module is used for pausing the equipment process when various liquid reagents need to be added or abnormal conditions are handled, and when the equipment is paused, timing of each module in operation is also paused.

[0029] The first centrifuge alarm module is used for alarming when the first centrifuge is not balanced, and the first centrifuge automatically stops working.

[0030] The first centrifuge abnormality handling module is used for performing a sample tube exit operation on a sample tube currently in the first centrifuge when the first centrifuge is not balanced, so that the equipment continues to work normally.

[0031] The centrifuge emptying module is used for performing a centrifuge emptying operation when the equipment fails, is powered off or a component is damaged, so that a test tube is left in the first centrifuge or the second centrifuge.

[0032] The air blowing abnormality processing module is configured to perform abnormality processing when the air blowing needle cannot normally fall, so that the device continues the air blowing processing;

[0033] The sample rack abnormality processing module is configured to prompt a worker to take out all samples on the sample rack and re-sample when the device malfunctions, is powered off or a component is damaged, causing the sample rack to be abnormal.

[0034] The enzyme label abnormality processing module is configured to perform abnormality processing and drag out the stuck enzyme label strip carrier when the enzyme label strip carrier in the micro-hole strip placing channel is stuck.

[0035] The application further provides a control method of the food safety detection workstation, which is realized based on the food safety detection workstation or the control system of the food safety detection workstation, and includes the following steps:

[0036] S1, replenish the pipette tip, the centrifugal tube and the second mechanical arm liquid adding needle with cleaning solution;

[0037] S2, drain the reagent in the front liquid adding liquid path, the rear liquid adding liquid path, the AB liquid and the cleaning solution, and drain the waste liquid in the corresponding reagent bottle into the drainage tank;

[0038] S3, create a test project, and create a standard curve of the test project by scanning a standard product curve two-dimensional code on a corresponding project reagent box or performing a standard product curve experiment;

[0039] S4, execute a sample detection experiment, first, perform sample scanning and sampling, set the number of sample tubes in a set of sampling racks and a preset test process to be executed, when the sample sampling is completed, the device automatically executes a detection process;

[0040] S5, generate a data analysis report according to the experimental results after the detection process is completed, and provide a printing report.

[0041] The embodiment of the application has the following technical effects:

[0042] The full-automatic food safety fluorescence detection and analysis workstation designed in the application integrates various modules and functions, can automatically complete all steps from sample pretreatment to detection and data report generation, the modules in the system are arranged according to the test process, form a smooth work chain, ensure effective connection and efficient operation of each step, and through the use of the first, second and third mechanical arms, automatic sample transfer is realized, manual intervention is reduced, work efficiency is improved, and uninterrupted continuous sampling can be realized, multiple samples can be processed at the same time, the detection efficiency is greatly improved, the detection period is shortened, and test errors are reduced. BRIEF DESCRIPTION OF DRAWINGS

[0043] In order to more clearly illustrate the specific embodiments of the present application or the technical solutions in the prior art, the following will briefly introduce the drawings required in the specific embodiments or prior art description. Obviously, the drawings described below are some embodiments of the present application, and all other embodiments obtained by a person of ordinary skill in the art without creative labor based on these drawings also belong to the scope of protection of the present application.

[0044] Figure 1 is a whole structure schematic diagram of a food safety detection work station provided by an embodiment of the present application;

[0045] Figure 2 is a structure schematic diagram of a food safety detection work station provided by an embodiment of the present application;

[0046] Figure 3 is a structure schematic diagram of a mechanical arm of a food safety detection work station provided by an embodiment of the present application;

[0047] Figure 4 is a flow chart of a control method of a food safety detection work station provided by an embodiment of the present application.

[0048] In the drawings:

[0049] 1-first processing module; 11-sample loading channel; 12-liquid adding module in front; 13-incubation system; 14-sample transfer mechanism; 15-first oscillator; 16-first centrifuge; 17-first mechanical arm; 2-second processing module; 21-second centrifuge; 22-second centrifuge balancing mechanism; 23-second oscillator; 24-air blowing system; 25-liquid adding module behind; 26-liquid needle cleaning mechanism; 27-waiting turntable; 28-second mechanical arm; 3-third detection module; 31-reagent placing rack; 32-micro-hole strip placing channel; 33-plate washing mechanism; 34-suction tip box mechanism; 35-suction tip removing mechanism; 36-third mechanical arm; 4-detection table. DETAILED DESCRIPTION

[0050] In order to make the purpose, technical solutions and advantages of the present application more clear, the technical solutions of the present application will be described clearly and completely below. Obviously, the described embodiments are only some of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by a person of ordinary skill in the art without creative labor also belong to the scope of protection of the present application.

[0051] An embodiment of the present application provides a food safety detection work station, Figure 1 is a whole structure schematic diagram of a food safety detection work station provided by an embodiment of the present application, Figure 2 is a structure schematic diagram of a food safety detection work station provided by an embodiment of the present application,Figure 3 is a structural schematic diagram of a mechanical arm of a food safety detection work station provided by an embodiment of the present application, referring to Figures 1-3 , specifically comprising: a detection table 4, and a pre-treatment module and a detection module arranged on the detection table 4;

[0052] The pre-treatment module is used for pre-treating a sample to obtain a to-be-detected solution for detection by the detection module;

[0053] The detection module comprises a third detection module 3, the third detection module 3 comprising a reagent placing rack 31, a micro-hole strip placing channel 32, a reagent adding module (not shown in the figure, in the present embodiment, the reagent adding module is located below the reagent placing rack 31), a plate washing mechanism 33, a suction tip box mechanism 34, a tip removing mechanism 35, a reading module (not shown in the figure, in the present embodiment, the reagent adding module is located below the reagent placing rack 31), and a third mechanical arm 36; the micro-hole strip placing channel 32 is used for placing enzyme-labeled micro-holes, the reagent placing rack 31 is arranged above the micro-hole strip placing channel 32 and is used for placing reagents required for detection, the reagent adding module is arranged between the micro-hole strip placing channel 32 and the reagent placing rack 31 and is used for adding reagents to the enzyme-labeled micro-holes to react with the to-be-detected solution, the plate washing mechanism 33 is arranged at one end of the micro-hole strip placing channel 32 close to the edge of the detection table 4 and is used for washing the enzyme-labeled micro-holes, the suction tip box mechanism 34 and the tip removing mechanism 35 are arranged above the micro-hole strip placing channel 32 and are arranged side by side on one side of the reagent placing rack 31, the suction tip box mechanism 34 is used for storing suction tips to replace the needle heads of liquid needles on the third mechanical arm 36, the tip removing mechanism 35 is used for removing used needle heads, the reading module is arranged between the micro-hole strip placing channel 32 and the reagent placing rack 31 and is used for measuring the results of the to-be-detected solution after reaction in the enzyme-labeled micro-holes, and the third mechanical arm 36 is used for sucking the lower liquid in a centrifugal tube containing a reaction solution from the pre-treatment module into the enzyme-labeled micro-holes or sucking reagents from the reagent placing rack 31 into the enzyme-labeled micro-holes.

[0054] In some embodiments, the pre-treatment module comprises a first treatment module 1 and a second treatment module 2;

[0055] The first treatment module 1 comprises a sample loading channel 11, a pre-adding module 12, an incubation system 13, a sample transfer mechanism 14, a first oscillator 15, a first centrifuge 16, and a first mechanical arm 17; the sample loading channel 11 is used for placing sample tubes, the sample transfer mechanism 14 is used for moving the sample tubes in the sample loading channel 11 to the pre-adding module 12 or the second treatment module, the pre-adding module 12 is arranged on one side of the sample transfer mechanism 14 so that the sample tubes supported by the sample transfer mechanism 14 can be moved to the corresponding needle positions of the pre-adding module 12, and the first mechanical arm 17 is used for transferring the sample tubes to the incubation system 13, the first oscillator 15, the first centrifuge 16, or the sample transfer mechanism 14;

[0056] The second processing module 2 comprises a second centrifuge 21, a second centrifuge balancing mechanism 22, a second oscillator 23, an air blowing system 24, a post-liquid adding module 25, a liquid needle cleaning mechanism 26, a waiting turntable 27 and a second mechanical arm 28; the second centrifuge balancing mechanism 22 is used for balancing operation of the second centrifuge 21, the air blowing system 24 is used for concentrating operation on sample pretreatment extract, the post-liquid adding module 25 is used for liquid adding operation on centrifuge tubes placed on the turntable of the post-liquid adding module 25, the liquid needle cleaning mechanism 26 is arranged at an intermediate position between the end of the sample transfer mechanism 14 and the air blowing system 24, so that the action distance is relatively the shortest and most reasonable, the liquid needle cleaning mechanism 26 is used for cleaning the liquid needle arranged on the second mechanical arm 28, the waiting turntable 27 is used for placing sample tubes and centrifuge tubes, and the second mechanical arm 28 is used for sucking supernatant from the sample tube in the sample transfer mechanism 14 into the centrifuge tube, and transferring the centrifuge tube into the second centrifuge 21, the second oscillator 23, the air blowing system 24, the post-liquid adding module 25 or the waiting turntable 27.

[0057] In some embodiments, the structure of the food safety detection work station is laid out and position-limited from the module motion track, physical avoidance and process travel track, and some self-defined position settings can also be made according to custom requirements and different products used by each module.

[0058] The first mechanical arm 17 is in a U-shaped structure, comprising two longitudinal beams, a cross beam and a gripper, the two longitudinal beams are each provided with a sliding rail, the two ends of the cross beam are fixed on the sliding rails of the two longitudinal beams, the cross beam is provided with a sliding rail, and the gripper is fixed on the sliding rail of the cross beam; the cross beam can slide back and forth along the longitudinal beams through the sliding rails of the longitudinal beams, and the gripper can slide left and right along the cross beam through the sliding rail of the cross beam.

[0059] The second mechanical arm 28 is in an L-shaped structure, comprising a longitudinal beam, a cross beam, a gripper and a liquid needle, the cross beam is provided with a sliding rail, one end of the longitudinal beam is fixed on the sliding rail of the cross beam, and the lower side of the longitudinal beam is a sliding rail; the gripper and the liquid needle are fixed on the sliding rail of the longitudinal beam, the liquid needle is arranged on one side close to the cross beam, and the gripper is arranged on the side away from the cross beam; the longitudinal beam can slide left and right along the cross beam through the sliding rail of the cross beam, and the gripper and the liquid needle can slide back and forth along the longitudinal beam through the sliding rail of the longitudinal beam.

[0060] The third mechanical arm 36 is in an L-shaped structure, comprising a longitudinal beam, a cross beam and a liquid needle, the longitudinal beam is provided with a sliding rail, one end of the cross beam is fixed on the sliding rail of the longitudinal beam, and the lower side of the cross beam is a sliding rail; the liquid needle is fixed on the sliding rail of the cross beam; the cross beam can slide back and forth along the longitudinal beam through the sliding rail of the longitudinal beam, and the liquid needle can slide left and right along the cross beam through the sliding rail of the cross beam.

[0061] In some embodiments, the first mechanical arm 17, the second mechanical arm 28 and the third mechanical arm 36 are all X, Y, Z three-axis mechanical arms, which are respectively used for transferring sample tubes, centrifugal tubes and sample liquids, wherein the first mechanical arm 17 is used to realize the transfer of sample tubes between functional modules; the second mechanical arm 28 is used to realize the transfer of centrifugal tubes and supernatant between functional modules; and the third mechanical arm 36 is used to realize the transfer operation of reaction liquid in the enzyme-labeled experiment link and the drop card operation of reaction liquid in the colloidal gold experiment link.

[0062] In some embodiments, the sample loading channel 11 is used to transport sample tubes from the sample loading channel 11 to the sample loading position to place, confirm and recover samples, and the sample tubes are placed on the sample loading rack for transportation. The sample loading channel 11 is equipped with 5 groups of sample loading racks, each group of sample loading racks is used to place samples of the same detection target, and each group of sample loading racks is equipped with 6 sample positions, which can realize simultaneous detection of 30 samples at a time. When the sample loading rack returns to the sample loading initial position, the system automatically prompts the user to continue the sample loading operation, thereby realizing uninterrupted continuous sample loading.

[0063] In some embodiments, the sample transfer mechanism 14 is used to move the sample tubes loaded with samples inside the device.

[0064] In some embodiments, the front liquid adding module 12 is used to add pretreatment liquid to the sample. Various pretreatment liquids are assembled according to the liquid path distribution table, and the sample pretreatment liquid adding step is completed.

[0065] In some embodiments, the food safety detection work station can carry 21 groups of single-channel liquid paths, including 13 groups of pretreatment liquid paths and 8 groups of post-treatment liquid paths (or classified as 5 groups of organic reagent liquid paths, 14 groups of conventional reagent liquid paths and 2 groups of microliter reagent liquid paths), each reagent has a dedicated liquid path to ensure the accuracy of liquid addition.

[0066] In some embodiments, the incubation system 13 is used for high-temperature incubation of sample pretreatment, and the incubation system 13 is equipped with 12 tube positions, which can satisfy simultaneous heating and incubation of 12 sample tubes. The incubation system 13 is also provided with an intelligent temperature control module, which can realize temperature adjustment of 0-120 degrees Celsius, and the temperature control accuracy can reach ±2 degrees Celsius.

[0067] In some embodiments, the first centrifuge 16 is equipped with 6 tube positions, which are used for centrifugal separation of samples. When the number of sample tubes is 1 or 5, sample tubes with equal volume and weight need to be placed in the balancing position of the sample loading rack or the large centrifuge to balance the first centrifuge 16.

[0068] In some embodiments, the second centrifuge 21 is equipped with 6 tube positions, which are used for centrifuging centrifugal tubes.

[0069] In some embodiments, the first oscillator 15 is used for sample pretreatment extraction oscillation. The first oscillator 15 is configured with 6 tube positions and adopts a “pendulum” oscillation mode. The sample to be tested can collide with the tube wall in the sample tube, and the grinding beads can also be used for sample grinding. The sample is fully broken, the extraction effect is better, and the time is shorter. When the liquid is oscillated, it also has the motion effect of vortex mixing.

[0070] In some embodiments, the second oscillator 23 is used for liquid mixing in the centrifugal tube. The second oscillator 23 is configured with 6 tube positions.

[0071] In some embodiments, the air blowing system 24 is used for sample pretreatment extraction concentration. The air blowing system 24 includes two air blowing modules, each of which includes 6 tube air blowing positions and 6 tube air blowing waiting positions. That is, 12 samples can be air blown at the same time and 12 samples can be waiting at the same time. The air blowing position and the air blowing waiting position are arranged on the rotating disc of the air blowing module in two rows. The air blowing position and the air blowing waiting position are converted by rotating the rotating disc, which greatly improves the turnover efficiency of the air blowing position and effectively alleviates the reduction of experimental efficiency caused by long air blowing time. The blowing pipe of the air blowing system 24 is provided with auxiliary hot air, which can accelerate the working efficiency of the air blowing link. The blowing pipe is segmented and lowered, and always maintains the minimum effective air blowing distance with the liquid level in the centrifugal tube, further improving the air blowing efficiency.

[0072] In some embodiments, the liquid needle of the second mechanical arm 28 is also provided with a liquid level detection system for detecting the liquid level in the centrifugal tube. The capacitive liquid level detection sensor is carried to make the liquid level detection more accurate. According to different liquids, the corresponding parameters are set to make the liquid needle and the liquid level always in the minimum effective contact distance, prevent the liquid needle from carrying liquid drops to cause inaccurate liquid quantity, and effectively prevent the liquid needle from being contaminated by the reagent.

[0073] In some embodiments, the liquid needle cleaning mechanism 26 is provided with 2 groups of needle cleaning systems for cleaning the liquid needle of the second mechanical arm 28. The “fountain type” liquid needle cleaning technology can effectively clean the outer wall and inner cavity of the liquid needle, realizing zero cross and zero pollution between different samples.

[0074] In some embodiments, the waiting rotating disc 27 is used for centrifugal tube conversion and waiting placement of the centrifugal tube to be tested in the sample pretreatment process. The waiting rotating disc 27 is provided with 48 tube positions.

[0075] In some embodiments, the micro-well strip placement channel 32 is equipped with 20 strips x 8 tube enzyme-labeled strip carriers, and 160 tube sample liquids can be simultaneously detected. The enzyme-labeled tube carrier passes through the reading module at a high speed. The reading module is a single probe detection module, which can solve the reading error caused by different reaction times between different samples, and the inconsistency of the result reading by multiple probes, thereby increasing the accuracy and consistency of the result reading. The reading module selects a filter for excitation light of 370 nm and a filter for receiving light of 525 nm.

[0076] The full-automatic food safety fluorescence detection and analysis workstation is designed to integrate various modules and functions, and can automatically complete all steps from sample pretreatment, detection to data report generation. The modules in the system are arranged according to the test process, forming a smooth work chain to ensure effective connection and efficient operation of each step. The use of the first, second and third mechanical arms realizes automatic transfer of samples, reduces manual intervention, improves work efficiency, and can continuously sample without interruption, process multiple samples at the same time, greatly improve the detection efficiency, shorten the detection cycle and reduce the test error.

[0077] The embodiment of the present application also provides a control system of a food safety detection workstation, which is realized based on the above food safety detection workstation and specifically includes the following modules.

[0078] The pipette tip setting module is used to set the initial use position of the pipette tip before the experiment starts.

[0079] The liquid path maintenance module is used to drain the waste liquid in the corresponding reagent bottle into the drain tank before the experiment starts.

[0080] The data analysis module is used to create a test project and build a standard curve of the test project. The project name and reagent related parameters can be directly obtained by scanning the two-dimensional code on the corresponding project reagent kit. The standard curve can be obtained by directly scanning the standard curve two-dimensional code on the reagent kit, or by performing a standard curve experiment.

[0081] The experiment running module is used to perform test setting, including making a standard curve experiment, placing the standard curve making reagent at the corresponding position on the food safety detection workstation according to the system prompt, placing the reagent, and then the device automatically enters the corresponding product standard curve making, obtains the standard curve data of the project, and after receiving the standard curve data, the system automatically saves the standard curve data with the corresponding name.

[0082] In some embodiments, the effective period of the standard curve data is calculated from the date of receiving the data, and when the standard curve data expires, the standard curve data is marked as red; the standard curve data of the same project can only be saved for one copy, and receiving the standard curve data of the project again will prompt to overwrite the old data, and if it is confirmed to be overwritten, the effective period of the standard curve data of the project will also be updated to the date of the new data;

[0083] The experiment running module is also used to execute a sample detection experiment. First, the sample is scanned and loaded, a number of sample tubes in a set of loading racks and a preset test process to be executed are set, and when the sample loading is completed, the device automatically executes the detection process.

[0084] In some embodiments, when some experimental items can be combined for pretreatment, pretreatment combined processing can also be performed; when the sample loading channel 11 status bar displays a green light indicating that the channel is idle, the sample can be loaded; when the sample loading channel 11 status bar displays a red light indicating that the channel is occupied, the sample cannot be loaded even if the channel is currently empty;

[0085] In some embodiments, whether to carry quality control samples for detection experiments can also be set according to needs. If it is selected to carry quality control samples for detection experiments, the system will display a quality control placement prompt, and the quality control samples are placed according to the prompt. At the same time, the microwell strip also needs to increase the quality control hole position;

[0086] The data analysis module is also used to generate a data analysis report according to the experimental results, and to provide a printed report;

[0087] In some embodiments, a data analysis report can be generated according to single or multiple groups of data, and the format of the report can be set by checking the preset display items;

[0088] The process detection module is used to track the status of each module of the device in real time during the experiment;

[0089] The device pause module is used to pause the device process when various liquid reagents need to be added or abnormal conditions need to be handled;

[0090] When the device is paused, the timing of each module in operation is also paused;

[0091] The first centrifuge alarm module is used to alarm when the first centrifuge 16 is not balanced, and the first centrifuge 16 automatically stops working;

[0092] The first centrifuge abnormality handling module is used to make the sample tubes currently in the first centrifuge 16 exit when the first centrifuge 16 is not balanced, so that the device continues to work normally;

[0093] It should be noted that the first centrifuge 16 needs to be reloaded after the abnormal processing is completed, and the sample of this group is discarded in this experiment;

[0094] The centrifuge module is emptied, which is used for centrifuge emptying operation when the equipment fails, power failure or component damage causes test tubes to remain in the first centrifuge 16 or the second centrifuge 21;

[0095] The air blowing abnormal processing module is used to execute abnormal processing when the air blowing needle cannot normally fall, so that the equipment continues to perform air blowing processing;

[0096] The sample holder abnormal processing module is used to prompt the staff to take out all the samples on the sample holder and reload the samples when the equipment fails, power failure or component damage causes the sample holder to be abnormal;

[0097] The enzyme label abnormal processing module is used to perform abnormal processing and drag out the enzyme label strip carrier when the enzyme label strip carrier in the micro-hole strip placing channel 32 is stuck in the in-out channel;

[0098] It should be noted that the enzyme label abnormal processing needs to be reloaded after the abnormal processing is completed, and the sample of this group is discarded in this experiment.

[0099] The embodiment provides a control method of a food safety detection workstation, which is realized based on the food safety detection workstation or the control system of the food safety detection workstation, Figure 4 is a flow chart of the control method of the food safety detection workstation provided by the embodiment of the application, and refers to Figure 4 , and includes the following steps:

[0100] S1, the pipette tip, the centrifuge tube and the second mechanical arm 28 liquid adding needle cleaning liquid are supplemented;

[0101] S2, the front liquid adding liquid path, the rear liquid adding liquid path, the AB liquid and the cleaning liquid are drained, and the waste liquid in the corresponding reagent bottle is drained into the drainage tank;

[0102] S3, the test project is created, and the standard curve two-dimensional code on the corresponding project reagent box or the standard curve experiment is performed to build the standard curve of the test project;

[0103] S4, the sample detection experiment is performed, first, the sample is scanned and loaded, the number of sample tubes in a set of loading racks and the preset test process to be executed are set, when the sample loading is completed, the equipment automatically executes the detection process;

[0104] S5, after the detection process is completed, the data analysis report is generated according to the experimental results, and the printing report is provided.

[0105] In some embodiments, the detection process of the food safety detection workstation in S4 comprises the following steps:

[0106] S41, pretreatment of the sample.

[0107] Specifically, it comprises:

[0108] S41.1, through the cooperation of the X-axis and Y-axis of the sample transfer mechanism 14, the sample loading rack moves left and right and forward and backward to the corresponding needle position of the front liquid adding module 12, up to 3 reagents can be added at a time, and multiple additions can be made according to the experimental process;

[0109] S41.2, transfer the sample tube to the first oscillator 15 through the first mechanical arm 17 to perform oscillation operation;

[0110] S41.3, transfer the sample tube to the incubation system 13 through the first mechanical arm 17 to perform incubation operation;

[0111] S41.4, transfer the sample tube to the sample loading rack through the first mechanical arm 17 and return to the initial position for standing;

[0112] S41.5, transfer the sample tube to the first centrifuge 16 through the first mechanical arm 17 to perform centrifugation operation;

[0113] S41.6, transfer the centrifugal tube on the waiting turntable 27 to the air blowing position of the air blowing system 24 through the gripper of the second mechanical arm 28;

[0114] S41.7, transfer the sample tube to the supernatant placement position (i.e. the sample tube placement rack on the lateral sliding rail of the sample transfer mechanism 14) on the sample transfer mechanism 14 through the first mechanical arm 17, the supernatant placement position moves laterally along the sliding rail to the end, and the supernatant in the sample tube is transferred to the centrifugal tube in the air blowing system 24 through the liquid needle of the second mechanical arm 28;

[0115] S41.8, after taking the supernatant, the supernatant placement position moves laterally along the sliding rail to the beginning, and the sample tube is transferred back to the sample loading rack through the first mechanical arm 17;

[0116] S41.9, the sample loading rack returns to the initial position;

[0117] S41.10, the air blowing position of the air blowing system 24 rotates to blow and concentrate the supernatant in the centrifugal tube;

[0118] S41.11, transfer the centrifugal tube to the rear liquid adding module 25 through the gripper of the second mechanical arm 28 to add liquid, and the liquid adding turntable of the rear liquid adding module 25 rotates to correspond the centrifugal tube to the corresponding needle position for liquid addition;

[0119] S41.12, transfer the centrifugal tube to the second shaker 23 by the gripper of the second mechanical arm 28 to perform the shaking operation;

[0120] S41.13, transfer the centrifugal tube to the second centrifuge 21 by the gripper of the second mechanical arm 28 to perform the centrifugal operation;

[0121] S41.14, transfer the centrifugal tube back to the waiting turntable 27 by the gripper of the second mechanical arm 28.

[0122] S42, perform post-detection on the sample.

[0123] Specifically includes:

[0124] S42.1, when the waiting turntable 27 rotates to the target position, move to the waiting turntable 27 by the third mechanical arm 36 to suck the lower layer liquid in the centrifugal tube and transfer it to the enzyme-labeled micro-hole;

[0125] S42.2, after the third mechanical arm 36 sucks the liquid in the centrifugal tube on the waiting turntable 27 in turn, move to the reagent position to suck the "standard point" reagent and transfer the liquid to the enzyme-labeled micro-hole;

[0126] S42.3, suck the enzyme conjugate reagent (located in the reagent placement rack 31) by the third mechanical arm 36 and transfer it to the enzyme-labeled micro-hole in turn;

[0127] S42.4, suck the antibody reagent (located in the reagent placement rack 31) by the third mechanical arm 36 and transfer it to the enzyme-labeled micro-hole in turn;

[0128] S42.5, move the micro-hole strip placement channel 32 base movement mechanism to below the enzyme-labeled micro-hole carrier to reciprocate to mix the added reagent in the enzyme-labeled micro-hole;

[0129] S42.6, move the enzyme-labeled micro-hole carrier to the incubation bin below the reagent placement rack 31 by the micro-hole strip placement channel 32 base movement mechanism to incubate and time;

[0130] S42.7, when the timing is over, move the enzyme-labeled micro-hole carrier to the initial position by the micro-hole strip placement channel 32 base movement mechanism;

[0131] S42.8, move the plate washing mechanism 33 above the enzyme-labeled strip carrier to perform the plate washing operation and lower to the inside of the enzyme-labeled micro-hole to suck the liquid;

[0132] S42.9, after the plate washing mechanism 33 finishes sucking the liquid, rise, move the enzyme-labeled strip carrier by the micro-hole strip placement channel 32 base movement mechanism, and align the hole positions of the enzyme-labeled micro-hole with the liquid adding needle of the plate washing mechanism 33 to add liquid;

[0133] S42.10, after adding liquid, the enzyme label strip carrier returns to the initial position;

[0134] S42.11, the plate washing mechanism 33 is lowered to the inside of the enzyme label micro-well to perform liquid suction, and the actions of S2.9-S2.11 are repeatedly executed 4 times;

[0135] S42.12, the micro-well strip placement channel 32 base movement mechanism moves the enzyme label strip carrier to below the reading module, and adds liquid through the reagent adding module;

[0136] S42.13, after adding liquid, the micro-well strip placement channel 32 base movement mechanism reciprocates to mix the added reagent;

[0137] S42.14, the micro-well strip placement channel 32 base movement mechanism moves the enzyme label strip carrier to the incubation bin below the reagent placement rack 31 to perform incubation and timing;

[0138] S42.15, after timing, the micro-well strip placement channel 32 base movement mechanism moves the enzyme label strip carrier to the initial position, and reciprocates to mix the liquid after reaction again;

[0139] S42.16, the micro-well strip placement channel 32 base movement mechanism moves the enzyme label strip carrier to below the reading module to read data.

[0140] The above operation is the complete experimental steps of the food safety workstation. According to the needs of different detection objects, the steps can be deleted, and for different detection objects, the time, number of times and the like required by each step operation are not the same. Each step is repeatedly executed according to the preset process of different detection objects, for example, after S41.1 is executed for the first group of samples, S41.2 is executed, at the same time, S41.1 is executed for the second group of samples, and so on, so as to realize continuous detection of multiple groups of detection objects and improve the detection throughput.

[0141] Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement for part or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solution deviate from the technical solutions of the embodiments of the present application.

Claims

1. A food safety testing workstation, characterized in that, include: The testing station (4), and the pre-processing module and the testing module set on the testing station (4); The pretreatment module is used to pretreat the sample to obtain the test solution for detection by the detection module; The pretreatment module includes a second processing module (2), which includes a waiting turntable (27) and a second robotic arm (28). The waiting turntable (27) is used to place centrifuge tubes, and the second robotic arm (28) is used to draw supernatant from the sample tube in the sample transfer mechanism (14) into the centrifuge tube and transfer the centrifuge tube into the waiting turntable (27). The second robotic arm (28) includes a liquid needle, and a capacitive liquid level detection system is also provided at the liquid needle of the second robotic arm (28) to detect the liquid level height in the sample tube, so that the liquid needle and the liquid surface are always at the minimum effective contact distance. The second processing module (2) further includes a second centrifuge (21), a second centrifuge balancing mechanism (22), a second oscillator (23), an air blowing system (24), a post-liquid addition module (25), and a liquid needle cleaning mechanism (26); the second centrifuge balancing mechanism (22) is used to balance the second centrifuge (21), the air blowing system (24) is used to concentrate the sample pretreatment extract, the post-liquid addition module (25) is used to add liquid to the centrifuge tubes placed on the turntable of the post-liquid addition module (25), and the liquid needle cleaning mechanism (26) is located at the middle position between the end of the sample transfer mechanism (14) and the air blowing system (24) for cleaning the liquid needles set on the second robotic arm (28). The second robotic arm (28) is also used to transfer the centrifuge tubes to the second centrifuge (21), the second oscillator (23), the air blowing system (24), or the post-liquid addition module (25); The detection module includes a third detection module (3), which includes a reagent rack (31), a micro-well strip placement channel (32), a reagent addition module, a plate washing mechanism (33), a tip-pipette mechanism (34), a tip removal mechanism (35), a reading module, and a third robotic arm (36). The micro-well strip placement channel (32) is used to place enzyme-labeled microwells. The reagent rack (31) is located above the micro-well strip placement channel (32) and is used to place the reagents required for detection. The reagent addition module is located between the micro-well strip placement channel (32) and the reagent rack (31) and is used to add reagents to the enzyme-labeled microwells to react with the solution to be detected. The plate washing mechanism (33) is located at one end of the micro-well strip placement channel (32) near the edge of the detection platform (4) and is used to clean the enzyme-labeled microwells. The tip-pipette mechanism (34) and the tip removal mechanism (35) are located at... Above the microwell strip placement channel (32), it is arranged side by side on one side of the reagent rack (31). The tip box mechanism (34) is used to store the tip for replacing the needle of the liquid needle on the third robotic arm (36). The tip removal mechanism (35) is used to remove the used needle. The reading module is set between the microwell strip placement channel (32) and the reagent rack (31) and is used to measure the result of the test solution after the reaction in the enzyme-labeled microwell. The microwell strip placement channel (32) includes a base movement mechanism. The base movement mechanism of the microwell strip placement channel (32) is used to move to the bottom of the enzyme-labeled microwell carrier and perform reciprocating motion to mix the reagent added to the enzyme-labeled microwell. The third robotic arm (36) is used to draw the lower layer liquid from the centrifuge tube containing the reaction solution in the pretreatment module into the enzyme-labeled microwell or draw the reagent from the reagent rack (31) into the enzyme-labeled microwell.

2. The food safety testing workstation according to claim 1, characterized in that, The preprocessing module includes a first processing module (1); The first processing module (1) includes a sample loading channel (11), a pre-liquid loading module (12), a sample transfer mechanism (14), a first oscillator (15), a first centrifuge (16), and a first robotic arm (17). The sample loading channel (11) is used to place sample tubes. The sample transfer mechanism (14) is used to move the sample tubes in the sample loading channel (11) to the pre-liquid loading module (12) or the second processing module (2). The pre-liquid loading module (12) is located on one side of the sample transfer mechanism (14) so ​​that the sample tubes supported by the sample transfer mechanism (14) can be moved to the corresponding needle position of the pre-liquid loading module (12). The first robotic arm (17) is used to transfer the sample tubes to the first oscillator (15), the first centrifuge (16), or the sample transfer mechanism (14).

3. A food safety testing workstation according to claim 2, characterized in that, The first processing module (1) further includes an incubation system (13), and the first robotic arm (17) is also used to transfer the sample tube into the incubation system (13).

4. A food safety testing workstation according to claim 2, characterized in that, The first robotic arm (17) has a U-shaped structure, including two longitudinal beams, one crossbeam and gripper. Both longitudinal beams are equipped with slide rails, and the two ends of the crossbeam are fixed on the slide rails of the two longitudinal beams respectively. The crossbeam is equipped with slide rails, and the gripper is fixed on the slide rail of the crossbeam. The crossbeam can slide back and forth along the longitudinal beam direction through the slide rail of the longitudinal beam, and the gripper can slide left and right along the crossbeam direction through the slide rail of the crossbeam. The second robotic arm (28) has an L-shaped structure, including a longitudinal beam, a transverse beam, a gripper and a liquid needle. A slide rail is provided on the transverse beam, one end of the longitudinal beam is fixed on the slide rail of the transverse beam, and a slide rail is located below the longitudinal beam. The gripper and the liquid needle are fixed on the slide rail of the longitudinal beam. The liquid needle is located on the side closer to the transverse beam, and the gripper is located on the side farther from the transverse beam. The longitudinal beam can slide left and right along the direction of the transverse beam through the slide rail of the transverse beam, and the gripper and the liquid needle can slide back and forth along the direction of the longitudinal beam through the slide rail of the longitudinal beam. The third robotic arm (36) has an L-shaped structure, including a longitudinal beam, a crossbeam and a liquid needle. The longitudinal beam is equipped with a slide rail, one end of the crossbeam is fixed on the slide rail of the longitudinal beam, the bottom of the crossbeam is a slide rail, and the liquid needle is fixed on the slide rail of the crossbeam. The crossbeam can slide back and forth along the longitudinal beam through the slide rail of the longitudinal beam, and the liquid needle can slide left and right along the crossbeam through the slide rail of the crossbeam.

5. A food safety testing workstation according to claim 3, characterized in that, The air blowing system (24) includes two air blowing modules. Each air blowing module consists of a 6-tube air blowing position and a 6-tube air blowing waiting position. The air blowing positions and the air blowing waiting positions are arranged in two rows on the turntable of the air blowing module. The positions of the air blowing positions and the air blowing waiting positions can be changed by rotating the turntable.

6. A food safety testing workstation according to claim 5, characterized in that, The air blowing module's blowing pipe descends in segments according to parameter settings, always maintaining the minimum effective air blowing distance from the liquid surface in the sample tube.

7. A control system for a food safety testing workstation, implemented based on the food safety testing workstation described in any one of claims 1-6, characterized in that, include: The data analysis module is used to create experimental projects before the experiment begins, and to establish standard curves for the experimental projects by scanning the standard curve QR codes on the corresponding project reagent kits or by conducting standard curve experiments; it is also used to generate data analysis reports based on the experimental results after the experiment, and to provide printed reports. The experimental operation module is used for setting up experiments, including creating standard curve experiments. Following system prompts, the system places the standard curve preparation reagents at the corresponding locations on the food safety testing workstation. After placing the reagents, the device automatically starts creating the standard curve for the corresponding product, acquiring the standard curve data for that project, and automatically saving the standard curve data with the corresponding name. It is also used to execute sample testing experiments. First, the system scans and loads the samples, sets the number of sample tubes in a loading rack, and sets the preset test procedures to be executed. Once sample loading is complete, the device automatically executes the testing process.

8. The control system of a food safety testing workstation according to claim 7, characterized in that, Also includes: The pipette tip setting module is used to set the initial use position of the pipette tip before the experiment begins; The liquid circuit maintenance module is used to drain the pre-liquid addition circuit, post-liquid addition circuit, AB solution and cleaning solution containing reagents before the experiment begins, and drain the waste liquid in the corresponding reagent bottle into the drain tank. The process monitoring module is used to track the status of each module of the equipment in real time during the experiment. The equipment pause module is used to pause the equipment process when it is necessary to add various liquid reagents or handle abnormal situations. When the equipment is paused, the timing of each running module will also be paused. The first centrifuge alarm module is used to issue an alarm when the first centrifuge is not properly aligned, and the first centrifuge will automatically stop working. The first centrifuge malfunction handling module is used to remove the sample tubes currently in the first centrifuge when the first centrifuge is not properly aligned, so that the equipment can continue to work normally. The centrifuge emptying module is used to empty the centrifuge when test tubes remain in the first or second centrifuge due to equipment failure, power outage, or component damage. The air-blowing anomaly handling module is used to perform anomaly handling when the air-blowing needle fails to fall normally, so that the equipment can continue to perform air-blowing. The sample rack malfunction handling module is used to prompt staff to remove all samples from the sample rack and reload them when the sample rack malfunctions due to equipment failure, power outage, or component damage. The enzyme labeling anomaly handling module is used to handle situations where the enzyme labeling strip holder gets stuck in the micro-well strip placement channel, and to pull out the stuck enzyme labeling strip holder.

9. A control method for a food safety testing workstation, implemented based on the control system of a food safety testing workstation according to any one of claims 1-6 or 7-8, characterized in that, Includes the following steps: S1. Replenish the cleaning solution for pipette tips, centrifuge tubes, and the second robotic arm's dispensing needle; S2. Perform the draining operation on the pre-liquid addition path, post-liquid addition path, AB solution and cleaning solution containing reagents, and drain the waste liquid in the corresponding reagent bottles into the draining tank. S3. Create a test project and establish the standard curve of the test project by scanning the standard curve QR code on the corresponding test kit or by conducting a standard curve experiment. S4. Perform sample testing experiment. First, scan the sample barcode and load it. Set the number of sample tubes in a set of sample loading racks and the preset test process to be executed. When the sample loading is completed, the equipment will automatically execute the testing process. S5. After the testing process is completed, generate a data analysis report based on the experimental results and provide a printable report.

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