A composition for improving eggshell quality and use thereof

Abstract

The application discloses a composition for improving eggshell quality and application thereof, and belongs to the field of feed. The composition comprises the following raw materials in parts by weight: 15-70 parts of a first component and 14-60 parts of a second component; the first component is at least one of ursolic acid and a ursolic acid derivative; and the second component is at least one of quercetagetin and a quercetagetin derivative. The application finds that the combination of ursolic acid and quercetagetin has a synergistic effect on improving egg quality of laying hens, and greatly reduces the proportion of dark-line eggs produced by the laying hens. The composition provided by the application is pure natural, safe and non-toxic, does not cause toxic side effects on animals, is beneficial to guaranteeing animal product safety, can be used as a safe, efficient, stable and controllable green and environment-friendly feed additive, and has a good application prospect in reducing the proportion of dark-line eggs produced by the laying hens.

Description

Technical Field

[0001] This invention belongs to the field of feed, specifically relating to a composition for improving eggshell quality and its application. Background Technology

[0002] In modern animal husbandry, egg quality is one of the key concerns for consumers. Eggs with dark patterns (irregular lines on the yolk surface) can negatively impact the appearance of the egg and consumers' willingness to buy.

[0003] Currently, products on the market that address the issue of dark-colored eggs include feed additives and biological agents. Many feed manufacturers have launched feed additives containing organic trace elements (such as zinc, manganese, and copper), rare earth elements, and serine; biological agents, such as bacteriophage preparations, improve eggshell quality and reduce the production of dark-colored eggs by inhibiting or killing microorganisms that cause diseases such as oviductitis.

[0004] Improper use of these products can lead to nutritional imbalances, affecting the overall health of laying hens; their high cost increases farming expenses. The effectiveness of biological agents can be influenced by various factors, such as strain adaptability, dosage, and timing of application; long-term use may lead to drug resistance.

[0005] To address these issues, there is an urgent need to develop products that improve eggshell quality, taking into full account the safety, effectiveness, and cost-effectiveness of these products. Summary of the Invention

[0006] The purpose of this invention is to overcome at least one deficiency of the prior art and to provide a composition for improving eggshell quality and its application.

[0007] The technical solution adopted in this invention is:

[0008] A composition comprising the following raw materials in parts by weight: 15-70 parts of a first component and 14-60 parts of a second component;

[0009] The first component is at least one of ursolic acid and ursolic acid derivatives;

[0010] The second component is at least one of quercetin and quercetin derivatives.

[0011] In some embodiments, the composition comprises the following parts by weight of raw materials: 20-65 parts of a first component and 15-40 parts of a second component.

[0012] In some embodiments, the composition comprises the following parts by weight of raw materials: 20-60 parts of a first component and 20-40 parts of a second component.

[0013] In some embodiments, the ursolic acid derivative includes ursolic acid acetate, and / or the quercitrin derivative includes quercitrin-7-O-glucoside and quercitrin fat-soluble derivatives.

[0014] The use of any of the compositions described above in any of the following:

[0015] (i) Application in the preparation of products for improving eggshell quality;

[0016] (ii) Applications in the preparation of feed, medicines, additives, food, health products, and cosmetics.

[0017] A product comprising any one of the compositions described above.

[0018] In some embodiments, the product includes feed, medicine, additives, food, health products, and cosmetics.

[0019] In some embodiments, when the product is feed, the content of the composition in the feed is 0.1-0.5 kg / t.

[0020] In some embodiments, when the product is an additive, the additive includes feed additives and food additives.

[0021] In some embodiments, the dosage form of the product is one of granules, tablets, capsules, powders, pills, suspensions, liquid preparations, and ointments.

[0022] In some embodiments, the method for preparing the composition includes the following steps:

[0023] (1) Add the first component and the second component to the biaxial high-efficiency mixer in the above proportions and mix them evenly;

[0024] (2) The mixture is added to a wet granulator for granulation to obtain a granular composition product.

[0025] In some embodiments, the rotational speed of the biaxial high-efficiency mixer is 500-1000 r / min.

[0026] In some embodiments, during granulation in a wet granulator, the horizontal stirring blade rotates at 50-100 r / min, the vertical blade rotates at 500-1000 r / min, and the time is 10-15 minutes.

[0027] The beneficial effects of this invention are:

[0028] This study found that the combined use of ursolic acid and quercetin in laying hens had a synergistic effect in improving egg quality, significantly reducing the proportion of eggs with dark spots.

[0029] The composition provided in this application is all-natural, safe and non-toxic, and will not cause toxic side effects to animals. It is beneficial to ensure the safety of animal products and can be used as a safe, efficient, stable and controllable green and environmentally friendly feed additive. It has a good application prospect in reducing the proportion of dark-colored eggs produced by laying hens. Attached Figure Description

[0030] Figure 1 This is a normal egg shape.

[0031] Figure 2 It is in the form of a dark-patterned egg.

[0032] Figure 3 The results show the egg production rate for each group. Detailed Implementation

[0033] The preferred embodiments of the present invention will be described in detail below with reference to specific examples. It should be noted that the specific embodiments given below are for illustrative purposes only and are not intended to limit the scope of the present invention. Those skilled in the art can make various modifications and substitutions to the present invention without departing from its spirit and intent.

[0034] A composition comprising the following raw materials in parts by weight: 15-70 parts of a first component and 14-60 parts of a second component;

[0035] The first component is at least one of ursolic acid and ursolic acid derivatives;

[0036] The second component is at least one of quercetin and quercetin derivatives.

[0037] In some embodiments, the composition comprises the following parts by weight of raw materials: 20-65 parts of the first component and 15-40 parts of the second component.

[0038] In some embodiments, the composition comprises the following parts by weight of raw materials: 20-60 parts of the first component and 20-40 parts of the second component.

[0039] In some embodiments, the ursolic acid derivative includes ursolic acid acetate, and / or the quercitrin derivative includes quercitrin-7-O-glucoside and quercitrin fat-soluble derivatives.

[0040] The above composition may be used in any of the following:

[0041] (i) Application in the preparation of products for improving eggshell quality;

[0042] (ii) Applications in the preparation of feed, medicines, additives, food, health products, and cosmetics.

[0043] A product comprising any one of the compositions described above.

[0044] In some embodiments, the product includes feed, medicine, additives, food, health products, and cosmetics.

[0045] In some embodiments, the dosage form of the product is one of granules, tablets, capsules, powders, pills, suspensions, liquid preparations, and ointments.

[0046] In some embodiments, when the product is feed, the content of the composition in the feed is 0.1-0.5 kg / t.

[0047] In some preferred embodiments, the feed is a special feed for laying hens, and the content of the composition in the feed is 0.1-0.5 kg / t.

[0048] In some embodiments, when the product is an additive, the additive includes feed additives and food additives.

[0049] In some preferred embodiments, the feed additive is a feed additive specifically for laying hens.

[0050] In some embodiments, the method for preparing the composition includes the following steps:

[0051] (1) Add the first component and the second component to the biaxial high-efficiency mixer in the above proportions and mix them evenly;

[0052] (2) The mixture is added to a wet granulator for granulation to obtain a granular composition product.

[0053] In some embodiments, the rotational speed of the biaxial high-efficiency mixer is 500-1000 r / min.

[0054] In some embodiments, during granulation in a wet granulator, the horizontal stirring blade rotates at 50-100 r / min, the vertical blade rotates at 500-1000 r / min, and the time is 10-15 minutes.

[0055] This application provides a safe, effective, and economical composition for resolving dark-colored eggs, containing ursolic acid and quercetin. Simultaneously, production personnel should strengthen feeding management to improve the overall health of laying hens and fundamentally reduce the production of dark-colored eggs.

[0056] Ursolic acid, also known as 3beta-Hydroxyurs-12-en-28-oic acid, has the chemical formula C2. 30 H 48 O3, structural formula is CAS No.: 77-52-1, Molecular Weight: 456.7 g / mol, EINECS No.: 201-034-0. Ursolic acid, also known as ursolic acid, is a natural triterpenoid compound with wide distribution in nature. It can be extracted and isolated from various medicinal plants and fruits and has a variety of biological effects, including sedation, anti-inflammation, antibacterial, anti-diabetic, anti-ulcer, and hypoglycemic effects.

[0057] Quercetagetin (QG), also known as 6-Hydroxyquercetin, is a 3,3',4',5,6,7-hexahydroxyflavone with the molecular formula C6. 15 H 10 O8, structural formula is CAS No.: 90-18-6. Quercetin, a flavonol compound, is one of the main components of marigolds (a plant in the Asteraceae family) and possesses certain antioxidant capabilities. The use of antioxidants can largely prevent and eliminate redox imbalances caused by oxidative stress, thus improving aquaculture efficiency. Quercetin, a natural antioxidant extracted from marigolds, has advantages such as safety and high efficacy.

[0058] Feeding trials have shown that laying hens fed with the composition of this application exhibit significantly improved production performance and markedly improved eggshell quality, specifically by reducing the rate of dark-colored eggs.

[0059] In practical applications, the ursolic acid and quercetin composition of this application can be added to the feed of laying hens as a feed additive. The specific amount added can be adjusted according to factors such as the breed of laying hens, the rearing environment, and the rearing stage.

[0060] Meanwhile, it is recommended to maintain a clean and hygienic rearing environment during the breeding process to ensure that laying hens receive sufficient nutrition and water. In addition, regular health checks on laying hens to promptly identify and address potential health problems are also important measures to help improve laying hen productivity and egg quality.

[0061] Unless otherwise specified, the techniques or conditions described in the following examples shall be performed in accordance with the techniques or conditions described in the literature in this field, or in accordance with the product instructions. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased through legitimate channels.

[0062] The quercetin (CAS: 90-18-6) and ursolic acid (CAS: 77-52-1) used in the following examples and comparative examples were finished products purchased from Chenguang Biotech Group Co., Ltd., wherein the purity of quercetin (CAS: 90-18-6) was 95%-98% and the purity of ursolic acid (CAS: 77-52-1) was 95%-98%.

[0063] Those skilled in the art can also use quercetin or ursolic acid extracted from various other plants, and if their purity is lower than that of the pure product, they can be converted to the equivalent of the pure product and prepared according to the formulation of this application to obtain the composition. Those skilled in the art should understand that the adjusted compositions are all within the scope of protection of this application.

[0064] The following description is based on specific embodiments.

[0065] The dual-shaft high-efficiency mixer used in the following examples is model SSHJ0.5, with a production capacity of 0.25T / batch and a power of 7.5KW.

[0066] Example 1

[0067] This embodiment provides a composition comprising the following components in parts by weight: 20 parts ursolic acid and 20 parts quercetin.

[0068] Example 2

[0069] This embodiment provides a composition comprising the following components in parts by weight: 20 parts ursolic acid and 40 parts quercetin.

[0070] Example 3

[0071] This embodiment provides a composition comprising the following components in parts by weight: 60 parts of ursolic acid and 20 parts of quercetin.

[0072] Example 4

[0073] This embodiment provides a composition comprising the following components in parts by weight: 60 parts ursolic acid and 40 parts quercetin.

[0074] Example 5

[0075] This embodiment provides a composition comprising the following components in parts by weight: 30 parts ursolic acid and 30 parts quercetin.

[0076] Example 6

[0077] This embodiment provides a composition comprising the following components in parts by weight: 65 parts ursolic acid and 25 parts quercetin.

[0078] Example 7

[0079] This embodiment provides a composition comprising the following components in parts by weight: 60 parts of ursolic acid and 24 parts of quercetin.

[0080] Example 8

[0081] This embodiment provides a composition comprising the following components in parts by weight: 60 parts of ursolic acid and 15 parts of quercetin.

[0082] Comparative Example 1

[0083] This embodiment provides a composition comprising the following components in parts by weight: 45 parts of ursolic acid and 0 parts of quercetin.

[0084] Comparative Example 2

[0085] This embodiment provides a composition comprising the following components in parts by weight: 0 parts ursolic acid and 30 parts quercetin.

[0086] Comparative Example 3

[0087] This embodiment provides a composition comprising the following components in parts by weight: 70 parts ursolic acid and 14 parts quercetin.

[0088] Comparative Example 4

[0089] This embodiment provides a composition comprising the following components in parts by weight: 15 parts ursolic acid and 60 parts quercetin.

[0090] Comparative Example 5

[0091] This embodiment provides a composition comprising the following components in parts by weight: 13 parts ursolic acid and 2 parts quercetin.

[0092] Comparative Example 6

[0093] This embodiment provides a composition comprising the following components in parts by weight: 8 parts ursolic acid and 35 parts quercetin.

[0094] Comparative Example 7

[0095] This embodiment provides a composition comprising the following components in parts by weight: 10 parts ursolic acid and 70 parts quercetin.

[0096] Comparative Example 8

[0097] This embodiment provides a composition comprising the following components in parts by weight: 75 parts ursolic acid and 5 parts quercetin.

[0098] Comparative Example 9

[0099] This embodiment provides a composition comprising the following components in parts by weight: 80 parts of ursolic acid and 15 parts of quercetin.

[0100] Comparative Example 10

[0101] This embodiment provides a composition comprising the following components in parts by weight: 85 parts ursolic acid and 15 parts quercetin.

[0102] The preparation methods for the examples and comparative examples are as follows:

[0103] (1) Add ursolic acid and marigold extract to a biaxial high-efficiency mixer in proportion and mix them evenly at a speed of 500-1000 r / min;

[0104] (2) Add the mixture to a wet granulator, and granulate it for 10-15 minutes at a horizontal stirring blade speed of 50-100 r / min and a vertical blade speed of 500-1000 r / min to obtain a granular composition product.

[0105] Experimental Example 1:

[0106] 1. Test materials and test methods

[0107] Three hundred healthy Hy-Line Grey laying hens aged 30 weeks with similar weights were randomly divided into 8 groups, with 3 replicates per group and 20 hens per replicate. The control group was fed a corn-soybean meal basal diet, which was a powdered compound feed formulated according to NRC (1994), and its composition and nutrient levels are shown in Table 1. The feeds in the examples and comparative examples were prepared by adding the above-mentioned composition to the corn-soybean meal basal diet at a rate of 500 g / t of basal diet. The experimental period was 28 days.

[0108] Table 1. Feed Formula and Nutrition Level

[0109] Items Content Corn 60.75 46 Soybean meal 25.25 Stone powder Flours 8.5 Soybean oil 0.5 Premix 5 Total 100 Nutritional levels Metabolizable energy ME / (kcal / kg) 3008 Crude protein CP (%) 16.42 Calcium (Ca%) 4.24 Total phosphorus (P%) 0.56 Available phosphorus AP (%) 0.41 Lysine (%) 1.15 Methionine (Met) (%) 0.47 Methionine (Met+cys) (%) 0.75

[0110] Premix: Provides per kilogram of feed the following: VA 1200 IU, VD 440 IU, VE 7.5 mg, VK 0.4 mg, VB1 0.3 mg, VB2 1.2 mg, niacin 5.5 mg, pantothenic acid 1.8 mg, VB6 0.55 mg, VH 0.03 mg, folic acid 0.25 mg, VB... 120.003 mg, Fe(FeSO4) 50 mg, Cu(CuSO4) 7 mg, Mn(MnSO4) 35 mg, Zn(ZnSO4) 35 mg, I(KI) 0.94 mg, Se(NaSeO3) 0.13 mg. These nutrient values ​​are calculated.

[0111] 2. Feeding methods

[0112] The experimental laying hens were housed in three-tiered cages. Before the experiment, the chicken coop and equipment were thoroughly rinsed and strictly disinfected. The coop was temperature-controlled using an electric intelligent heater, employing continuous lighting, natural ventilation, and longitudinal negative pressure ventilation. During the experiment, the lighting, relative humidity, and temperature inside the coop were controlled according to the requirements for laying hen husbandry. The coop was cleaned regularly, and immunizations were administered according to the standard immunization schedule. Throughout the experiment, the chickens had free access to feed and water. Temperature and humidity inside the coop were recorded daily at 08:00, 12:00, and 18:00.

[0113] 3. Measurement Indicators and Methods

[0114] 3.1 Dark Pattern Egg Rate

[0115] Count the number of dark-patterned eggs and divide by the total number of eggs.

[0116] The identification of eggs with dark markings is mainly based on visual inspection. Specifically, the egg is placed in a light box or on a lamp base, and strong light is used to penetrate the egg. Defects in the eggshell and interior are then observed with the naked eye. Because dark markings alter the structure of the surrounding eggshell, this alteration may cause differences in the translucency of the eggshell under light, thus revealing the dark markings.

[0117] Figure 1 This is a normal egg shape. Figure 2 It is in the form of a dark-patterned egg.

[0118] 4. Test Results

[0119] The results are shown in Table 2. As can be seen from Table 2, compared with the control group and each comparative example, the addition of the feed additive compositions of each embodiment to the diet during the experiment reduced dark-spotted eggs.

[0120] Table 2 Statistical Results of Dark-Spotted Eggs

[0121]

[0122]

[0123] Table 2 shows that the blank control group was fed a corn-soybean meal basal diet, and under normal feeding conditions, there was a certain rate of dark-spotted eggs (41%). Comparative Example 1's composition contained only ursolic acid, which had a certain effect on reducing the rate of dark-spotted eggs, resulting in a final rate of 32%. Comparative Example 2's composition contained only quercetin, which had a certain effect on reducing the rate of dark-spotted eggs, resulting in a final rate of 34%.

[0124] In Comparative Examples 3-10, the compositions contained ursolic acid and quercetin, which showed a certain effect on reducing the rate of dark spots. However, the ratio range of the compositions was not optimized, and their effect on reducing dark spots was comparable to that of compositions containing only ursolic acid or quercetin.

[0125] Table 2 shows that the compositions of Examples 1-8 are more effective than those of Comparative Examples 1-10 in reducing the rate of eggs with dark spots; among them, the composition of Example 3 has the best effect on reducing the rate of eggs with dark spots. The rate of eggs with dark spots in the comparative examples is on average about 9%-10% higher than that in the examples.

[0126] The optimized compositions of ursolic acid and quercetin in Examples 1-8 exhibit unexpected effects, significantly reducing the rate of eggs with dark spots. This demonstrates that the combination of ursolic acid and quercetin in a specific ratio produces a synergistic effect. During the research and development process, the inventors also experimented with compositions combining ursolic acid and isochlorogenic acid, as well as compositions combining quercetin and isochlorogenic acid, but the results in reducing the rate of eggs with dark spots were not as good as the composition of ursolic acid and quercetin presented in this application.

[0127] Since the percentage of eggs with dark markings is closely related to egg quality, addressing this issue can increase the commercial value of eggs, improve their overall appearance, and enhance their market competitiveness. Furthermore, dark-marked eggs are often accompanied by reduced shell strength, making them more prone to breakage. Improving the percentage of eggs with dark markings can reduce egg losses during transportation and storage, thereby lowering production costs.

[0128] In summary, the ursolic acid and quercetin composition of this application can improve egg quality while reducing the rate of eggs with dark spots.

[0129] Experimental Example 2

[0130] This feeding trial was divided into a blank control group and an experimental group. The experimental subject was Fengda Black Chicken. The mass ratio of ursolic acid to marigold extract was 30:30 (Example 5), the dosage was 500g / ton, and the trial period was 45 days. The experimental results are shown in Table 3.

[0131] Table 3 Statistical Results

[0132]

[0133]

[0134] After feeding the compound product, the failure rates of the two groups were 41% and 55%, respectively. The failure rate of the experimental group with the addition of quercetin (QG) and ursolic acid was lower than that of the blank control group, with a difference of 14%. The rate of dark-spotted eggs in the experimental group was 8% lower than that in the control group.

[0135] Experimental Example 3

[0136] This feeding trial was a long-term feeding trial, and the subjects were Hy-Line Grey laying hens. The trial was divided into a blank control group and experimental groups 1 and 2. Experimental group 1 was supplemented with quercetin at a dosage of 500 g / ton for 60 days. Experimental group 2 was supplemented with a combination of ursolic acid and quercetin (mass ratio of 65:25, Example 6) at a dosage of 500 g / ton for 490 days from the onset of laying to the late laying period.

[0137] During the study, it was found that long-term addition of quercetin (experimental group 1) had no positive effect on egg production. However, the combination product of ursolic acid and quercetin (experimental group 2) showed a better effect on egg production compared with the blank control group and experimental group 1.

[0138] The egg production rate results for each group are shown below. Figure 3 . Figure 3 The addition of the composition described in this application shows that it can increase egg production.

[0139] Table 4 Statistical Results of Dark-Spotted Eggs

[0140]

[0141] Table 4 shows that the rate of dark-spotted eggs in the blank control group was 58.26%. The group fed with the ursolic acid + quercetin combination had a dark-spotted egg rate of 27.78%, significantly lower than the control group. The group fed with quercetin alone had a dark-spotted egg rate of 46.25%, also lower than the control group, but higher than the group fed with the ursolic acid and quercetin combination. Table 4 results indicate that the combination of ursolic acid and quercetin can significantly reduce the proportion of dark-spotted eggs laid by laying hens.

[0142] Test Example 4

[0143] In this feeding trial, the selected subjects were Hy-Line Grey laying hens in the late laying period. The trial was divided into experimental group 3 and experimental group 4. Experimental group 3 was a compound of ursolic acid and quercetin (13:2, comparative example 5), and experimental group 2 was a compound of ursolic acid and quercetin (75:5, comparative example 8). The dosage was 500g / ton.

[0144] The experimental results are shown in Table 5:

[0145] Table 5 Statistical Results of Dark-Spotted Eggs

[0146]

[0147] Table 5 shows that the compositions of Comparative Examples 5 and 8 have a certain effect on reducing the rate of eggs with dark spots. However, since the ratio of ursolic acid and marigold extract is not the preferred ratio of this application, the effect on reducing the rate of eggs with dark spots is not as good as the effect of the embodiments of this application.

[0148] The above is a further detailed description of the present invention and should not be considered as a limitation on the specific implementation of the present invention. For those skilled in the art, simple deductions or substitutions without departing from the concept of the present invention are all within the protection scope of the present invention.

Claims

1. A composition, characterized in that, The composition comprises the following parts by weight of raw materials: 15-70 parts of the first component and 14-60 parts of the second component; The first component is at least one of ursolic acid and ursolic acid derivatives; the ursolic acid derivative is selected from ursolic acid acetate; The second component is at least one of quercitrin and quercitrin derivatives; the quercitrin derivatives are selected from quercitrin-7-O-glucoside and quercitrin fat-soluble derivatives.

2. The composition according to claim 1, characterized in that, The composition comprises the following raw materials in parts by weight: 20-65 parts of the first component and 15-40 parts of the second component.

3. The composition according to claim 1, characterized in that, The composition comprises the following parts by weight of raw materials: 20-60 parts of the first component and 20-40 parts of the second component.

4. Use of the composition according to any one of claims 1-3 in the preparation of feed for improving eggshell quality.

5. A product characterized in that, The product comprises the composition according to any one of claims 1-3.

6. The product according to claim 5, characterized in that, The products include animal feed.

7. The product according to claim 6, characterized in that, The content of this composition in the feed is 0.1-0.5 kg / t.

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