A whitening and freckle-removing active composition, preparation method and application in cosmetics

Abstract

The present invention relates to the field of biomedicine technology, and more particularly to a whitening and freckle-removing active composition, a preparation method, and application thereof in cosmetics. The whitening and freckle-removing active composition comprises a Rhodiola rosea (RHODIOLA CRENULATA) root extract, a MORUS ALBA (MORUS ALBA) extract, and hexapeptide-2. The whitening and freckle-removing active composition has multi-target effects, and the components are reasonably matched, thereby achieving significant synergistic effects in terms of antioxidant effects and whitening and freckle-removing effects.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedicine, and in particular to a whitening and freckle-removing active composition, a preparation method and application thereof in cosmetics. Background Art

[0002] With the improvement of people's living standards and the pursuit of beauty, it is becoming more and more common for the skin to lose its brightness and even develop spots due to factors such as ultraviolet radiation, genetics, bad living habits, and aging. The market demand for whitening and freckle removal products continues to increase.

[0003] Skin color is primarily determined by the amount of melanin in the skin. Melanin is a ubiquitous biological pigment. The synthesis, transport, and metabolism of melanin in melanocytes are complex biochemical processes involving multiple enzymes. Skin whitening requires addressing melanin synthesis, melanosome transport, metabolism, oxidative stress, and environmental stimuli such as UV radiation to achieve the desired effect. Skin whitening primarily addresses three key areas: inhibiting melanin synthesis, regulating melanin metabolic pathways, and promoting melanin metabolism. Numerous ingredients with whitening and freckle-removing properties have been discovered and applied, such as niacinamide, tranexamic acid, and phenylethylresorcinol, which inhibit tyrosinase activity. Furthermore, natural plant extracts with antioxidant properties and salicylic acid, which promotes keratin renewal, are also being used to accelerate melanin metabolism.

[0004] As stated in the background technology section of Chinese invention patent CN118717601A, years of research and use have revealed that achieving effective whitening results with a single active whitening ingredient is difficult. Most active whitening ingredients, while highly active, are also extremely unstable. Therefore, the development of current whitening products requires selecting appropriate whitening ingredients based on multiple whitening target mechanisms. Furthermore, it is crucial to rationally combine these ingredients based on an understanding of their physical and chemical properties and safety profiles, verifying their safety and efficacy, and developing truly effective, gentle, and safe whitening products that truly meet consumer demand for whitening. Summary of the Invention

[0005] In order to solve the above technical problems, the purpose of the present invention is to provide a whitening and freckle-removing active composition, a preparation method and its application in cosmetics. The whitening and freckle-removing active composition has multi-target effects, and the components are reasonably matched, which can play a significant synergistic role in antioxidant effect and whitening and freckle-removing effect.

[0006] In order to achieve the above technical effects, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a whitening and freckle-removing active composition comprising the following raw materials: Rhodiola rosea (RHODIOLA CRENULATA) root extract and MORUS ALBA (MORUS ALBA) extract.

[0008] Preferably, the whitening and freckle-removing active composition further comprises the following raw material: hexapeptide-2.

[0009] Preferably, the whitening and freckle-removing active composition comprises the following raw materials in parts by mass: 2 to 15 parts of Rhodiola rosea (RHODIOLA CRENULATA) root extract and 1 to 8 parts of MORUS ALBA (MORUS ALBA) extract.

[0010] Furthermore, the whitening and freckle-removing active composition further comprises the following raw materials in parts by mass: 4 parts of hexapeptide-2.

[0011] Preferably, in the whitening and freckle-removing active composition provided by the present invention, the MORUS ALBA extract is any one of MORUS ALBA leaf extract, MORUS ALBA branch extract or MORUS ALBA root bark extract.

[0012] In a second aspect, the present invention further provides a method for preparing a whitening and freckle-removing active composition, which specifically comprises the following steps:

[0013] The extract of Rhodiola rosea (RHODIOLA CRENULATA) root was prepared by water extraction or alcohol extraction.

[0014] Preferably, the preparation method comprises the following steps:

[0015] Mulberry (MORUS ALBA) leaf extract was prepared by fermentation method.

[0016] Further preferably, the fermentation method is:

[0017] S1: Select fresh, pest-free mulberry leaves, dry them to a moisture content of 60% to 70%, and then cut them;

[0018] S2: Mixing the cut mulberry leaves with the fermentation broth at an inoculation ratio of 3% to 10%, wherein the fermentation broth is 3% to 10% of the mass of the mulberry leaves, and stirring thoroughly to allow the microorganisms in the fermentation broth to evenly adhere to the mulberry leaves. Fermenting at 30°C to 37°C for 3 to 9 days;

[0019] S3: After the fermentation is completed, the fermented mulberry leaves are decocted and extracted at a solid-liquid ratio of 1:20-50 for 1-4 hours, and the extraction is performed 2-3 times. The extracts from multiple extractions are mixed, and the filtrate is filtered and collected after mixing, and freeze-dried to obtain a mulberry (MORUS ALBA) leaf extract.

[0020] More preferably, the fermentation broth contains Candida aeruginosa, Bacillus cereus and Aspergillus oryzae, and the ratio of the number of viable bacteria of the three species is 2-3:4-6:1-3.

[0021] In a third aspect, the present invention further provides a use of the aforementioned whitening and freckle-removing active composition in skin care products and cosmetics.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] First, the composition provided by the present invention includes Rhodiola rosea (RHODIOLA CRENULATA) root extract, Morus alba (MORUS ALBA) leaf extract, and Hexapeptide-2. The Rhodiola rosea root extract is rich in salidrosides and tyrosol, exhibiting antioxidant and anti-free radical properties. It can reduce damage to skin cells caused by factors such as ultraviolet rays, thereby inhibiting melanin production at the root. The flavonoids and phenolic compounds in the mulberry extract also possess antioxidant and tyrosinase inhibitory properties, reducing melanin synthesis. Hexapeptide-2, as an antagonist of the α-melanocyte stimulating hormone (α-MSH) receptor, blocks the binding of α-MSH to its receptor, reducing tyrosinase activity and further inhibiting melanin production. These three ingredients work synergistically to achieve multiple skin-whitening and freckle-removing effects, resulting in a more pronounced effect than any single ingredient alone. Secondly, the Rhodiola rosea root extract and mulberry extract in the present composition are both derived from natural plants. These natural ingredients are relatively mild in nature, minimizing skin irritation and damage while achieving skin-whitening and freckle-removing effects. Even sensitive skin has a high tolerance, reducing the likelihood of adverse reactions such as allergies. Finally, the composition provided by this invention also utilizes a specific fermentation method to prepare a Morus alba leaf extract. Using Candida aeruginosa, Bacillus cereus, and Aspergillus oryzae at a specific ratio of viable bacteria, this method not only increases the content and activity of the extract's active ingredients but also makes the extract milder, further enhancing product safety. Overall, this active whitening and anti-freckle composition has broad market application prospects. DETAILED DESCRIPTION

[0024] The following examples are only used to more clearly illustrate the technical solutions of the present invention and are therefore only examples and are not intended to limit the scope of protection of the present invention. The specific embodiments listed in the present invention are only examples of the present invention, and the present invention is not limited to the specific embodiments described below. For those skilled in the art, any equivalent modifications and substitutions made to the embodiments described below are also within the scope of the present invention. Therefore, equivalent transformations and modifications made without departing from the spirit and scope of the present invention should all be included within the scope of the present invention.

[0025] To better illustrate the present invention, numerous specific details are provided in the following detailed description. Those skilled in the art will appreciate that the present invention can be practiced without certain specific details. In other embodiments, methods, means, equipment, and steps well known to those skilled in the art are not described in detail in order to highlight the main purpose of the present invention.

[0026] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those generally understood by those skilled in the art. Unless otherwise specified, the units used in this specification are international standard units, and the numerical values ​​and numerical ranges appearing in the present invention are all understood to include inevitable systematic errors in industrial production. Unless otherwise specified, the experimental methods used in the following implementations are all conventional methods; the materials, reagents or instruments used, if the manufacturer is not specified, are all reagents and materials available from commercial channels. If the specific conditions are not specified in the examples, they are all carried out according to conventional conditions or the conditions recommended by the manufacturer. At the same time, the present invention does not limit the source of the raw materials used. Unless otherwise specified, the raw materials used in the present invention are all common commercial products in this technical field.

[0027] It should be noted that the raw materials and equipment used in the specific embodiments of the present invention are all known products and were obtained by purchasing commercial products. Among them, the preparation process of modern Chinese medicine preparations made from the Chinese medicine composition of the present invention is mature and can be prepared by referring to conventional methods in the prior art.

[0028] Example 1

[0029] This embodiment provides a whitening and freckle-removing active composition, which comprises the following raw materials in parts by weight: 5 parts of Rhodiola rosea (RHODIOLA CRENULATA) root extract and 5 parts of MORUS ALBA (MORUS ALBA) leaf extract. The present invention also provides a preparation method thereof, specifically as follows:

[0030] A1: Preparation of Rhodiola rosea (RHODIOLA CRENULATA) root extract:

[0031] The Rhodiola rosea root extract is prepared using an aqueous extraction method. Specifically, the roots are first rinsed with clean water to remove impurities and dust, then cut into small segments approximately 1 to 2 centimeters in length. The cut root segments are placed in a container and extracted twice, using a solid-liquid ratio of 1:30 for 30 minutes. The extracts are then combined and the residue removed to obtain an aqueous Rhodiola rosea root extract. The aqueous extract is then concentrated and dried under reduced pressure to obtain the Rhodiola rosea root extract.

[0032] A2: Preparation of Mulberry (MORUS ALBA) Leaf Extract:

[0033] S1: Select fresh, pest-free mulberry leaves, dry them to a moisture content of 60% to 70%, and then cut them;

[0034] S2: Mix the cut mulberry leaves with fermentation liquid at an inoculation ratio of 5%, wherein the fermentation liquid accounts for 3% to 10% of the mass of the mulberry leaves, and stir thoroughly to make the microorganisms in the fermentation liquid evenly adhere to the mulberry leaves. Ferment at 34°C for 5 days. The strain contained in the fermentation liquid is Candida aeruginosa, and the concentration of the fermentation liquid is 1*10 5 CFU / mL.

[0035] S3: After the fermentation is completed, the fermented mulberry leaves are decocted and extracted at a solid-liquid ratio of 1:30 for 2 hours, and the extraction is performed twice. The extracts from the two extractions are mixed, and the filtrate is filtered and collected after mixing and freeze-dried to obtain the mulberry (MORUS ALBA) leaf extract.

[0036] Example 2

[0037] This embodiment provides a whitening and freckle-removing active composition, which differs from Example 1 in that the present invention uses an alcohol extraction method to prepare the Rhodiola rosea (RHODIOLA CRENULATA) root extract, specifically:

[0038] With reference to the aforementioned embodiment 1, the difference between this embodiment and embodiment 1 is that:

[0039] Wash and cut the roots of Rhodiola rosea into small pieces, put them into a container, and add 95% by volume of

[0040] The extraction is carried out with ethanol-water solution, and ultrasonic extraction is performed after soaking for 12 hours at a solid-liquid ratio of 1:30. The ultrasonic power is usually 500W and the extraction time is 25 minutes. After extraction, the extract is concentrated and dried to obtain the Rhodiola rosea (RHODIOLA CRENULATA) root extract.

[0041] Example 3

[0042] This embodiment provides a whitening and freckle-removing active composition, which comprises the following raw materials in parts by weight: 5 parts of Rhodiola rosea (RHODIOLA CRENULATA) root extract and 5 parts of MORUS ALBA (MORUS ALBA) leaf extract. The present invention also provides a preparation method thereof, which specifically comprises the following steps:

[0043] A1: Rhodiola rosea (RHODIOLA CRENULATA) root extract, prepared as described in Example 2;

[0044] A2: Preparation of MORUS ALBA leaf extract. The difference between the preparation method and that of Example 2 is that the concentration of the fermentation liquid is 1*10 5 CFU / mL, the bacteria species contained in the fermentation broth were Candida aeruginosa and Bacillus cereus, and the ratio of the number of viable bacteria between the two was 2:4.

[0045] Example 4

[0046] This embodiment provides a whitening and freckle-removing active composition, which comprises the following raw materials in parts by weight: 5 parts of Rhodiola rosea (RHODIOLA CRENULATA) root extract and 5 parts of MORUS ALBA (MORUS ALBA) leaf extract. The present invention also provides a preparation method thereof, which specifically comprises the following steps:

[0047] A1: Rhodiola rosea (RHODIOLA CRENULATA) root extract, prepared as described in Example 2;

[0048] A2: Preparation of MORUS ALBA leaf extract. The difference between the preparation method and that of Example 2 is that the concentration of the fermentation liquid is 1*10 5 CFU / mL, the bacteria species contained in the fermentation broth were Candida aeruginosa and Aspergillus niger, and the ratio of the number of viable bacteria between the two was 2:1.

[0049] Example 5

[0050] This embodiment provides a whitening and freckle-removing active composition, which comprises the following raw materials in parts by weight: 5 parts of Rhodiola rosea (RHODIOLA CRENULATA) root extract and 5 parts of MORUS ALBA (MORUS ALBA) leaf extract. The present invention also provides a preparation method thereof, which specifically comprises the following steps:

[0051] A1: Rhodiola rosea (RHODIOLA CRENULATA) root extract, prepared as described in Example 2;

[0052] A2: Preparation of MORUS ALBA leaf extract. The difference between the preparation method and that of Example 2 is that the concentration of the fermentation liquid is 1*10 5 CFU / mL, the bacteria species contained in the fermentation broth were Candida aeruginosa and Aspergillus oryzae, and the ratio of the number of viable bacteria between the two was 2:1.

[0053] Example 6

[0054] This embodiment provides a whitening and freckle-removing active composition, which comprises the following raw materials in parts by weight: 5 parts of Rhodiola rosea (RHODIOLA CRENULATA) root extract and 5 parts of MORUS ALBA (MORUS ALBA) leaf extract. The present invention also provides a preparation method thereof, which specifically comprises the following steps:

[0055] A1: Rhodiola rosea (RHODIOLA CRENULATA) root extract, prepared as described in Example 2;

[0056] A2: Preparation of MORUS ALBA leaf extract. The difference between the preparation method and that of Example 2 is that the concentration of the fermentation liquid is 1*10 5 CFU / mL, the bacterial species contained in the fermentation broth were Candida aeruginosa, Bacillus cereus and Aspergillus oryzae, and the ratio of the number of viable bacteria among the three was 2:4:1.

[0057] Example 7

[0058] This embodiment provides a whitening and freckle-removing active composition, which comprises the following raw materials in parts by weight: 5 parts of Rhodiola rosea (RHODIOLA CRENULATA) root extract and 5 parts of MORUS ALBA (MORUS ALBA) leaf extract. The present invention also provides a preparation method thereof, which specifically comprises the following steps:

[0059] A1: Rhodiola rosea (RHODIOLA CRENULATA) root extract, prepared as described in Example 2;

[0060] A2: Preparation of MORUS ALBA leaf extract. The difference between the preparation method and that of Example 2 is that the concentration of the fermentation liquid is 1*10 5 CFU / mL, the bacterial species contained in the fermentation broth were Candida aeruginosa, Bacillus cereus and Aspergillus niger, and the ratio of the number of viable bacteria among the three was 2:4:1.

[0061] Example 8

[0062] This embodiment provides a whitening and freckle-removing active composition, which comprises the following raw materials in parts by weight: 5 parts of Rhodiola rosea (RHODIOLA CRENULATA) root extract and 5 parts of Morus alba (MORUS ALBA) branch extract. The present invention also provides a preparation method thereof, which specifically comprises the following steps:

[0063] A1: Rhodiola rosea (RHODIOLA CRENULATA) root extract, prepared as described in Example 2;

[0064] A2: Prepare mulberry (MORUS ALBA) branch extract. The preparation method thereof is similar to that of Example 2, but differs from Example 1 in that the raw material used in this example is mulberry branch.

[0065] Test Example 1

[0066] Based on Examples 1 to 8, the present invention conducted the following tests on the whitening and freckle-removing active compositions prepared in the aforementioned Examples (respectively recorded as Composition 1 to Composition 8):

[0067] 1.1 DPPH clearance rate

[0068] DPPH is a stable free radical. When antioxidants are present, they react with DPPH, changing its color from dark purple to light yellow. The DPPH radical scavenging rate can be calculated by measuring the change in absorbance. Therefore, in this example, Compositions 1 to 8 were tested using the DPPH scavenging rate test method in GB / T 39100-2020. The specific method is not repeated here. Each experiment was repeated three times. The experimental results are shown in Table 1.

[0069] 1.2 Inhibitory activity against tyrosinase

[0070] Tyrosinase is a key enzyme in the biosynthesis of melanin in the skin. In a phosphate solution at pH 6.8, tyrosinase catalyzes the conversion of dopa to dopaquinone, with absorbance measured at 475 nm on a spectrophotometer. Raw materials that inhibit tyrosinase activity can reduce the conversion of dopa to dopaquinone, thereby lowering absorbance. Changes in absorbance can be used to assess the inhibitory effect of cosmetic raw materials on tyrosinase activity. Therefore, in this example, compositions 1 to 8 were tested for their inhibitory activity against tyrosinase, with reference to T / SHRH 015-2018. The experimental results are shown in Table 1.

[0071] 1.3 Effects on melanin content in B16-F10 cells

[0072] B16-F10 cells were seeded into culture dishes or culture flasks and cultured in DMEM medium containing 10% fetal bovine serum in a 37°C, 5% CO2 incubator. The cells were used for experiments when they grew to the logarithmic growth phase.

[0073] Experimental groups used Compositions 1 to 8, and a blank control group was also established. Treatment solutions from Compositions 1 to 8 were added to each experimental group. After incubation for 24 hours, the culture medium was discarded, and the cells were rinsed two to three times with PBS. Cell lysis buffer was added and incubated at room temperature for two hours to fully lyse the cells and release melanin. The cell lysate was transferred to a centrifuge tube and centrifuged. The supernatant was collected and its absorbance was measured spectrophotometrically at 475 nm. The relative change in melanin content in each experimental group relative to the blank control group was calculated. The experimental results are shown in Table 1.

[0074] Table 1 Test results of compositions 1 to 8

[0075] DPPH clearance rate (%) Tyrosinase inhibitory activity (%) Relative content of melanin (%) Example 1 56.41±2.11 35.34±1.21 -21.07 Example 2 61.02±1.97 44.08±0.96 -34.12 Example 3 61.29±2.33 56.31±3.04 -33.43 Example 4 63.82±3.54 53.55±2.01 -41.02 Example 5 61.09±2.08 58.24±3.31 -32.19 Example 6 91.61±2.90 89.37±1.13 -79.31 Example 7 75.69±5.01 71.34±2.92 -49.23 Example 8 48.36±3.23 39.11±2.09 -18.30

[0076] The above experimental results indicate that different preparation methods for Rhodiola rosea (RHODIOLA CRENULATA) root extract and Morus alba (MORUS ALBA) leaf extract significantly influence the whitening and anti-freckle effects of the composition. Furthermore, Example 8 also demonstrates that the effectiveness of mulberry branch extract under the formulation of the present invention is inferior to that of mulberry leaf extract. The experimental results of Examples 3-7 also demonstrate that the type of fermentation bacteria used to ferment Morus alba leaf extract significantly influences the antioxidant and whitening effects of the composition. Based on these experimental results, the optimal fermentation bacteria combination is determined to be that of Example 6.

[0077] Example 9

[0078] In this example, based on the above-mentioned Example 6, the inventors further studied the mass ratio of the root extract of Rhodiola rosea (RHODIOLA CRENULATA) and the leaf extract of MORUS ALBA. The evaluation criteria refer to the above-mentioned Test Example 1. The experimental results are shown in Table 2:

[0079] Table 2 Comparison of composition, oxidation effect and whitening effect of control 1 to control 4

[0080] Rhodiola rosea (RHODIOLA CRENULATA) root extract mass percentage Mulberry (MORUS ALBA) leaf extract mass percentage DPPH clearance rate (%) Tyrosinase inhibitory activity (%) Relative content of melanin (%) Control 1 2 8 86.05±1.28 85.35±2.68 -60.12 Control 2 4 6 89.37±1.97 87.19±1.02 -70.49 Control 3 6 4 93.20±2.31 92.15±0.91 -89.92 Control 4 8 2 88.31±2.69 90.35±1.72 -83.4

[0081] The above experimental results show that the composition provided by the present invention is a combination of Rhodiola rosea (RHODIOLA CRENULATA) root extract and MORUS ALBA (MORUS ALBA) leaf extract, and the mass ratio of the two also has a significant impact on the overall antioxidant effect and whitening effect of the composition. When the mass ratio of the two is 6:4, the antioxidant effect and whitening effect of the composition can reach the best.

[0082] Example 10

[0083] Hexapeptide-2, also known as whitening peptide, is a small molecule peptide composed of six amino acids. It is synthesized artificially and has a compact molecular structure. The amino acid sequence includes glycine, glutamic acid, and proline. As an antagonist of the α-melanocyte-stimulating hormone (α-MSH) receptor, hexapeptide-2 competitively binds to receptors on melanocytes, blocking the binding of α-MSH to its receptors and thereby inhibiting melanin synthesis. It also reduces the activity of enzymes involved in melanin synthesis (such as tyrosinase), reducing melanin accumulation in the skin.

[0084] Therefore, based on the above Example 6, this example further explores the combination of the composition and hexapeptide-2:

[0085] Please refer to Example 6. The raw materials (in parts by mass) used in the compositions provided in this example are as follows:

[0086] Control 5: 4 parts of Rhodiola rosea (RHODIOLA CRENULATA) root extract, prepared as in Example 6; 4 parts of Mulberry (MORUS ALBA) leaf extract, prepared as in Example 6; 2 parts of Hexapeptide-2;

[0087] Control 6: 8 parts of Rhodiola rosea (RHODIOLA CRENULATA) root extract, prepared as in Example 6; 8 parts of Mulberry (MORUS ALBA) leaf extract, prepared as in Example 6;

[0088] Control 7: 4 parts of hexapeptide-2;

[0089] The above composition was tested for its antioxidant activity and whitening and freckle removal effect. The experimental results are shown in Table 3:

[0090] Table 3 This example provides the test results of the antioxidant activity and whitening and freckle removal effects of each composition

[0091] DPPH clearance rate (%) Tyrosinase inhibitory activity (%) Relative content of melanin (%) Control 5 96.31±1.64 98.31±2.61 -94.19 Control 6 90.12±2.30 88.23±3.09 -76.20 Control 7 78.01±2.43 95.03±3.21 -89.62

[0092] The experiment shows that the addition of hexapeptide-2 can produce a good synergistic effect with the composition provided in this application (Rhodiola rosea (RHODIOLACRENULATA) root extract and mulberry (MORUS ALBA) leaf extract).

[0093] The above embodiments are intended only to illustrate the technical solutions of the present invention and are not intended to limit the scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art will appreciate that the technical solutions of the present invention may be modified or replaced with equivalents without departing from the spirit and scope of the technical solutions of the present invention, and such modifications are intended to be encompassed by the claims of the present invention. Any techniques, shapes, and structures not described in detail herein are well known.

Claims

1. A whitening and freckle-removing active composition, characterized in that: The invention comprises the following raw materials in parts by mass: 2 to 15 parts of Rhodiola rosea (RHODIOLA CRENULATA) root extract, 1 to 8 parts of MORUS ALBA extract, and 1 to 4 parts of hexapeptide-2, wherein the MORUS ALBA extract is a MORUS ALBA leaf extract, wherein: The Rhodiola rosea (RHODIOLA CRENULATA) root extract is prepared by alcohol extraction; The mulberry (MORUS ALBA) leaf extract is prepared by a fermentation method, wherein the fermentation bacteria are a mixture of Candida albicans, Bacillus cereus and Aspergillus oryzae, and the ratio of the number of live bacteria of the three bacteria is 2:4:

1.

2. The method for preparing a whitening and freckle-removing active composition according to claim 1, wherein: The following steps are involved: Rhodiola rosea (RHODIOLA CRENULATA) root extract was prepared by alcohol extraction method; Mulberry (MORUS ALBA) leaf extract was prepared by fermentation method; Wherein, the fermentation method is: S1: Select fresh, pest-free mulberry leaves, dry them to a moisture content of 60% to 70%, and then cut them; S2: Mixing the cut mulberry leaves with the fermentation broth at an inoculation ratio of 3% to 10%, wherein the fermentation broth is 3% to 10% of the mass of the mulberry leaves, and stirring thoroughly to allow the microorganisms in the fermentation broth to evenly adhere to the mulberry leaves. Fermenting at 30°C to 37°C for 3 to 9 days; S3: After the fermentation is completed, the fermented mulberry leaves are decocted and extracted at a solid-liquid ratio of 1:20-50. The extraction time is 1-4 hours, and the extraction is performed 2-3 times. The extracts of multiple extractions are mixed, and the filtrate is filtered and collected after mixing, and freeze-dried to obtain the mulberry (MORUS ALBA) leaf extract.

3. Use of the whitening and freckle-removing active composition according to claim 1 in cosmetics.