A microbial organic fertilizer containing fullerene C60 and preparation method thereof

Abstract

The invention provides a microbial organic fertilizer containing fullerene C60 and a preparation method thereof, and relates to the technical field of microbial organic fertilizers. The preparation raw materials of the microbial organic fertilizer containing fullerene C60 include biologically improved composite bacterial liquid, modified fullerene C60 and organic auxiliary materials, which are mixed in a weight ratio of 1-3:5-10:10-25. The preparation method includes mixing the biologically improved composite bacterial liquid, the modified fullerene C60 and the organic auxiliary materials. The prepared microbial organic fertilizer has good stability, dispersibility or absorption efficiency.

Description

Technical Field

[0001] The invention relates to the technical field of microbial organic fertilizer, and in particular to a microbial organic fertilizer containing fullerene C60 and a preparation method thereof. Background Art

[0002] Saline-alkali soil is a global soil problem, and its soil salinization phenomenon poses a serious threat to agricultural production. Saline-alkali land has the characteristics of high salinity, poor soil structure, low fertility, soil nutrient imbalance, decreased organic matter content, structural damage, severe compaction, acidification, secondary salinity damage, and shallowing of the tillage layer, which makes it difficult for crops to grow normally, and the crop yield is low and the quality is poor. The soil conditions can no longer meet the requirements of developing modern green, high-quality, and efficient agriculture, and to a certain extent, it has accelerated the deterioration of the ecological environment. Therefore, improving saline-alkali land, improving soil fertility, and increasing the area of ​​arable land have become important issues in the development of global agriculture. Microbial organic fertilizer is a fertilizer product that combines microorganisms with organic fertilizers. It combines the dual advantages of microorganisms and organic fertilizers, and has both the improvement effect of microorganisms and the nutrient supply capacity of organic fertilizers. Microbial organic fertilizers can improve soil structure, increase soil fertility, reduce soil salinity, and promote crop growth. However, although traditional microbial organic fertilizers can improve soil structure and improve soil fertility to a certain extent, for severely salinized soils, the microorganisms in some existing microbial organic fertilizers have weak survival ability in saline-alkali environments and are difficult to play an effective role in saline-alkali soils, which limits the application effect of microbial organic fertilizers in saline-alkali land improvement. Its improvement effect is often limited and it is difficult to meet the needs of crop growth. Some organic fertilizers release nutrients slowly and cannot meet the large demand for nutrients in the rapid growth stage of crops, affecting crop yield and quality. With the influence of global climate change and unreasonable irrigation, the area of ​​saline-alkali land continues to expand, posing a serious threat to agricultural production. In addition, as a by-product of plant protein, dry bean dregs have relatively stable ingredients, high protein content and are easy to control. Compared with dry distiller's grains, dry bean dregs are relatively clean during production and storage, and the risk of microbial contamination is lower. Therefore, during the enzymatic hydrolysis process, the conversion and release of nutrients are more controllable, which helps to prepare a fermentation medium with stable quality and rich nutrition, while also reducing the contamination of harmful bacteria and pathogens, and improving the purity and safety of the fermentation medium. Therefore, there is an urgent need in the market for fertilizers that can effectively improve saline-alkali land and increase soil fertility. Summary of the invention

[0003] The present invention provides a microbial organic fertilizer containing fullerene C60 and a preparation method thereof, so as to solve the problems raised in the above-mentioned background technology.

[0004] In order to solve the above technical problems, the present invention discloses a microbial organic fertilizer containing fullerene C60 and a preparation method thereof. The microbial organic fertilizer containing fullerene C60 comprises raw materials for preparation including biologically improved composite bacterial liquid, modified fullerene C60 and organic auxiliary materials.

[0005] Furthermore, the raw materials for preparation include 1-5 parts of biologically improved composite bacterial liquid, 8-15 parts of modified fullerene C60 and 15-30 parts of organic auxiliary materials by weight.

[0006] Furthermore, the preparation method of the microbial organic fertilizer containing fullerene C60 comprises: mixing, by weight, 1-5 parts of the prepared biologically improved composite bacterial solution, 8-15 parts of modified fullerene C60 and 15-30 parts of organic auxiliary materials.

[0007] Furthermore, the preparation method of the modified fullerene C60 comprises: mixing 40-50 parts of ethylenediamine, 100-120 parts of ethanol and 20-50 parts of water by weight to obtain a solution A, weighing 0.2-0.5 parts of fullerene C60 and 2-5 parts of NaOH respectively and adding them to 200-250 parts of toluene to obtain a solution B, stirring 20-25 parts of solution A and 2-5 parts of solution B under nitrogen protection to react, standing to separate the liquids, removing the lower layer of liquid by suction filtration, washing, and vacuum drying to obtain the modified fullerene C60;

[0008] Preferably, the reaction time under nitrogen protection is 7-10 days, and the stirring speed is 120-150r / min;

[0009] Preferably, the standing time is 24-30h;

[0010] Preferably, washing with 95% ethanol;

[0011] Preferably, the vacuum drying temperature is 60-80°C and the time is 48-55h.

[0012] Furthermore, the preparation method of the bio-improved composite bacterial liquid includes: uniformly mixing methylotrophic Bacillus fermentation liquid, polymyxa fermentation liquid, cerevisiae fermentation liquid, Rhodopseudomonas fermentation liquid, Phanerochaete chrysosporium fermentation liquid, Lactobacillus plantarum fermentation liquid and Aspergillus niger fermentation liquid in a volume ratio of 1.5:1.5:1.5:1:1:0.5:5 to obtain the bio-improved composite bacterial liquid.

[0013] Furthermore, the preparation method of the organic auxiliary material includes: mixing fresh chicken manure, dry bean dregs and rice husks, wherein the weight ratio of fresh chicken manure to rice husks is 5:4, adding dry bean dregs with a weight of 5% of the weight of fresh chicken manure, adding a biologically improved composite bacterial liquid with a weight of 3% of the weight of fresh chicken manure, composting and fermenting to obtain the organic auxiliary material.

[0014] Furthermore, the rice husk has a particle size of 60-80 meshes, and the fermentation time is 20-25 days to obtain an organic auxiliary material.

[0015] Furthermore, the preparation methods of the methylotrophic Bacillus fermentation broth, the polymyxa Bacillus fermentation broth, the cerevisiae fermentation broth, the Rhodopseudomonas fermentation broth, the Phanerochaete chrysosporium fermentation broth and the Lactobacillus plantarum fermentation broth include: in a sterile environment, picking up activated single colonies, inoculating them into a liquid fermentation medium, and culturing them at a constant temperature to obtain a fermentation broth; the preparation method of the Aspergillus niger fermentation broth includes: in a sterile environment, collecting the activated Aspergillus niger spores with sterile water, inoculating them into a liquid fermentation medium, and culturing them at a constant temperature.

[0016] Furthermore, the activation is carried out in a sterile environment by coating methylotrophic Bacillus, Paenibacillus polymyxa, Saccharomyces cerevisiae, Rhodopseudomonas, Phanerochaete chrysosporium, Lactobacillus plantarum and Aspergillus niger on a solid activation culture medium plate, and culturing at a constant temperature of 28-30° C. for 24-96 hours to obtain activated colonies and Aspergillus niger spores;

[0017] Preferably, the liquid fermentation medium comprises dry bean dregs enzymatic hydrolyzate.

[0018] Furthermore, the preparation method of the dry bean dregs enzymatic hydrolyzate comprises mixing the dry bean dregs and tap water in a weight ratio of 1:20, adjusting the pH to 9 with an ammonia solution, bathing at 80° C. for 60 minutes, adding alkaline protease at 300 U / g-dry bean dregs for enzymatic hydrolysis at 50° C. for 24 hours, and centrifuging to obtain a 5% amino acid-rich dry bean dregs enzymatic hydrolyzate.

[0019] Compared with the prior art, the present invention provides a microbial organic fertilizer containing fullerene C60 and a preparation method thereof, which has the following beneficial effects:

[0020] (1) Fullerene C60 has a unique cage structure and excellent physical and chemical properties, such as good chemical stability, good mechanical stability, corrosion resistance, and biological activities such as antioxidant and antiviral. These properties enable microbial organic fertilizers containing fullerene C60 to remain stable in the soil environment and not easily decomposed or ineffective; biological activities such as antioxidant and antiviral can improve the stress resistance of crops and enhance their ability to adapt to adverse conditions such as salinity and drought;

[0021] (2) Phanerochaete chrysosporium is a white-rot fungus with high lignin degradation ability, which converts difficult-to-degrade macromolecules into small molecules that are easily absorbed by plants. This degradation effect not only helps to shorten the composting time, but also improves the quality and fertility of the compost.

[0022] (3) Lactobacillus plantarum is a probiotic that is beneficial to plant growth. It can improve the soil microecological environment and promote the growth and development of plant roots. The dry climate, strong winds and sand, and harsh environment in saline-alkali land lead to poor growth and development of crops. The addition of probiotics such as Lactobacillus plantarum can improve the growth of crops by improving their resistance to stress (such as resistance to wind and sand, drought, and pests and diseases).

[0023] (4) Amino-modified fullerene C60 improves the water solubility and biocompatibility of fullerene C60, which is beneficial to the better dispersion of fullerene C60 and bio-modified composite bacterial solution and avoids agglomeration;

[0024] (5) The bio-improved composite bacterial solution, modified fullerene C60, and organic auxiliary materials are mixed together at a certain formula ratio to give full play to their respective advantages, form a synergistic effect, make various components evenly distributed in the microbial organic fertilizer, and improve the overall effect of the microbial organic fertilizer;

[0025] (6) By analyzing the correlation between rice husk and fermentation time in advance, composting fermentation can be better performed when organic auxiliary materials are prepared, so that the preparation of organic auxiliary materials can be measured based on the target fermentation time, which provides convenience for the preparation of organic auxiliary materials, makes full use of composting fermentation materials, avoids the waste of materials due to insufficient composting fermentation, improves the preparation rate of organic auxiliary materials, and also ensures the quality of organic auxiliary materials, avoids the problem of quality control of organic auxiliary materials caused by insufficient composting fermentation, and affects the preparation of microbial organic fertilizer. In addition, by using the correction factor to correct the target fermentation time, the influence of different rice husk particle sizes on composting fermentation is fully considered, and the accuracy of the target fermentation time is improved, so that the corrected target fermentation time can be used as a reference when preparing organic auxiliary materials, thereby improving the preparation efficiency of organic auxiliary materials, providing guarantee for the preparation of organic auxiliary materials, and then guaranteeing the preparation of microbial organic fertilizer. DETAILED DESCRIPTION

[0026] The preferred embodiments of the present invention are described below. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0027] In addition, in the present invention, the descriptions of "first", "second", etc. are only used for descriptive purposes, and do not specifically refer to the order or sequence, nor are they used to limit the present invention. They are only used to distinguish components or operations described with the same technical terms, and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the technical solutions and technical features between the various embodiments can be combined with each other, but they must be based on the ability of ordinary technicians in the field to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0028] Unless otherwise specified, the examples and comparative examples are parallel tests with the same components, component contents, preparation steps and preparation parameters. The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the following examples are analytical reagents (AR) unless otherwise specified. Parts are parts by weight unless otherwise specified, and the materials are purchased from commercial channels.

[0029] Fullerene C60 was purchased from Sigma Aldrich, CAS No.: 99685-96-8, molecular weight: 720.64, purity: 99.5%.

[0030] Methylotrophic Bacillus, Paenibacillus polymyxa, Saccharomyces cerevisiae, Rhodopseudomonas, Phanerochaete chrysosporium, Lactobacillus plantarum and Aspergillus niger were purchased from China General Microbiological Culture Collection Center (CGMCC) and China Center for Type Culture Collection (CCTCC).

[0031] Example 1

[0032] The preparation method of microbial organic fertilizer containing fullerene C60 comprises the following specific steps:

[0033] 1. Preparation of biologically improved composite bacterial solution:

[0034] (1) Preparation of dry bean dregs enzymatic hydrolysate:

[0035] The dry bean dregs were uniformly mixed with tap water in a weight ratio of 1:20, adjusted to pH 9 with ammonia solution, and placed in a water bath at 80°C for 60 minutes. After adding alkaline protease at a rate of 300 U / g dry bean dregs for enzymatic hydrolysis at 50°C for 24 hours, the enzymatic hydrolyzate was centrifuged to obtain a 5% concentration of dry bean dregs hydrolyzate rich in amino acids.

[0036] (2) Preparation of culture medium

[0037] a. Methylotrophic Bacillus solid activated medium: 10 g / L tryptone, 5 g / L yeast powder, 10 g / L NaCl, 20 g / L agar, 3 g / L methanol, and the rest is tap water, natural pH, sterilize at 121°C for 15 minutes, and pour into plates.

[0038] b. Paenibacillus polymyxa solid activated medium: 10 g / L tryptone, 5 g / L yeast powder, 10 g / L NaCl, 20 g / L agar, the rest is tap water, natural pH, sterilize at 121°C for 15 minutes, and pour into plates.

[0039] c. Solid activated culture medium for Saccharomyces cerevisiae: 20 g / L glucose, 10 g / L yeast powder, 20 g / L peptone, 20 g / L agar, and the rest is tap water, natural pH, sterilize at 121°C for 15 minutes, and pour into a plate.

[0040] d. Rhodopseudomonas solid activated medium: yeast powder 10g / L, peptone 10g / L, agar 20g / L, sodium chloride 5g / L, the rest is tap water, adjust the initial pH to 7.0, sterilize at 121℃ for 15 minutes, and pour into the plate.

[0041] e. Solid activated medium for Phanerochaete chrysosporium: 40 g / L glucose, 10 g / L yeast powder, 5 g / L peptone, 20 g / L agar, 1 g / L potassium dihydrogen phosphate, 0.5 g / L magnesium sulfate heptahydrate, and the rest is tap water. The initial pH is adjusted to 4.5-5.5, and the medium is sterilized at 121°C for 15 minutes before pouring into a plate.

[0042] f. plant lactobacillus solid activation medium: glucose 20g / L, yeast powder 10g / L, peptone 20g / L agar 20g / L, ammonium citrate trihydrate 2g / L, sodium acetate 5g / L, Tween 80 is 1mL / L, magnesium sulfate heptahydrate 0.5g / L, manganese sulfate tetrahydrate 0.25g / L, dipotassium hydrogen phosphate 2g / L, the rest is tap water, adjust the initial pH 6.0-6.5, after sterilization at 121°C for 15 minutes, pour the plate.

[0043] g. Aspergillus niger solid activated medium: 200 g / L potato extract, 20 g / L glucose, 20 g / L agar, the rest is tap water, natural pH, sterilize at 121°C for 15 minutes, and pour into plates.

[0044] h. Liquid fermentation medium for methylotrophic Bacillus, Paenibacillus polymyxa and Rhodopseudomonas: 40 g / L glucose, 5 g / L peptone, 10 g / L beef extract, 5 g / L yeast powder, 5 g / L calcium carbonate, and the rest is dry bean dregs enzymatic hydrolyzate. The initial pH is adjusted to 7.2-7.6 and sterilized at 121°C for 15 minutes.

[0045] i. Liquid fermentation medium of Phanerochaete chrysosporium: 40 g / L glucose, 5 g / L peptone, 10 g / L beef extract, 5 g / L yeast powder, 5 g / L calcium carbonate, and the rest was dry dregs enzymatic hydrolyzate, citric acid (1 M concentration) was added to adjust the initial pH to 4.5-5.5, and sterilized at 121 ° C for 15 minutes;

[0046] j. Liquid fermentation medium for Lactobacillus plantarum: 40 g / L glucose, 5 g / L peptone, 10 g / L beef extract, 5 g / L yeast powder, 5 g / L calcium carbonate, and the rest is dry bean dregs enzymatic hydrolyzate. Diamine hydrogen citrate is added to adjust the initial pH to 6.5, and sterilized at 121°C for 15 minutes.

[0047] k. Liquid fermentation medium for brewer's yeast: 80 g / L glucose, 5 g / L yeast powder, 20 g / L peptone, 5 g / L calcium carbonate, and the rest is dry bean dregs enzymatic hydrolyzate. The initial pH is adjusted to 7.2-7.6, and sterilized at 121°C for 15 minutes.

[0048] 1. Aspergillus niger liquid fermentation medium: glucose 30g / L, peptone 5g / L, (ammonium sulfate 5g / L, calcium carbonate 5g / L, the rest is dry bean dregs enzymatic hydrolyzate, adjust the initial pH to 7.2-7.6, and sterilize at 121℃ for 15 minutes.

[0049] (3) Bacterial Activation

[0050] In a sterile environment, methylotrophic Bacillus, Paenibacillus polymyxa, Saccharomyces cerevisiae, Rhodopseudomonas, Phanerochaete chrysosporium, Lactobacillus plantarum and Aspergillus niger are spread on a solid activated culture medium plate, and cultured at a constant temperature of 28-30° C. for 24-96 hours to obtain activated colonies and Aspergillus niger spores.

[0051] (4) Fermentation culture

[0052] Under a sterile environment, the colonies obtained after the activation of the strain (3) are selected and inoculated into a liquid fermentation medium, and cultured at a constant temperature of 150 rpm and 28° C. for 24-72 hours to obtain the above-mentioned colony fermentation liquid.

[0053] (5) Mixing

[0054] The methylotrophic Bacillus fermentation broth, Paenibacillus polymyxa fermentation broth, Saccharomyces cerevisiae fermentation broth, Rhodopseudomonas fermentation broth, Phanerochaete chrysosporium fermentation broth, Lactobacillus plantarum fermentation broth and Aspergillus niger fermentation broth obtained above were uniformly mixed in a volume ratio of 1.5:1.5:1.5:1:1:0.5:5 to obtain a bio-improved composite bacterial liquid.

[0055] 2. Preparation of organic auxiliary materials:

[0056] Fresh chicken manure, dry bean dregs and rice husks are mixed, and the weight ratio of fresh chicken manure to rice husks is 5:4. Among them, the particle size of rice husks is 60-80 meshes. The dry bean dregs used are added in a ratio of 5% of the mass of fresh chicken manure. The C / N of the mixture obtained after mixing is 15. Bio-improvement composite bacterial liquid is added, and composting and fermentation for 20 days to obtain organic auxiliary materials.

[0057] 3. Preparation of modified fullerene C60:

[0058] 40 mL of ethylenediamine was measured and 96 mL of a mixture of ethanol and water (ethanol: water = 5: 1) was prepared. The two were miscible to obtain solution A for use; 200 mg of fullerene C60 and 2.0 g of NaOH were weighed and added to 200 mL of toluene to obtain solution B for use; under stirring, solution A was added dropwise to solution B using a constant pressure funnel, and the reaction was carried out under nitrogen protection for 7 days. After standing, the liquid was separated using a separatory funnel, the lower layer of liquid was taken and filtered, and the solid was vacuum dried at 60°C for 24 hours, and then the solid was washed with a mixture of ethanol and water (ethanol: water = 6: 1), and then the solid was vacuum dried at 60°C for 24 hours.

[0059] 4. Preparation of microbial organic fertilizer containing fullerene C60:

[0060] Five parts of the prepared biologically improved composite bacterial liquid, 15 parts of modified fullerene C60 and 30 parts of organic auxiliary materials are uniformly mixed to obtain the microbial organic fertilizer containing fullerene C60 of the present invention.

[0061] Example 2

[0062] Steps 1-3 are the same as those in Example 1, except that a microbial organic fertilizer containing fullerene C60 is prepared:

[0063] One part of the prepared biologically improved composite bacterial liquid, 10 parts of modified fullerene C60 and 20 parts of organic auxiliary materials are uniformly mixed to obtain the microbial organic fertilizer containing fullerene C60 of the present invention.

[0064] Example 3

[0065] Steps 1-3 are the same as those in Example 1, except that a microbial organic fertilizer containing fullerene C60 is prepared:

[0066] Three parts of the prepared biologically improved composite bacterial liquid, eight parts of modified fullerene C60 and 15 parts of organic auxiliary materials are uniformly mixed to obtain the microbial organic fertilizer containing fullerene C60 of the present invention.

[0067] Further, based on Examples 1-3, when preparing organic auxiliary materials, a correlation analysis is performed in advance on rice husks and fermentation time, including: dividing the weight and particle size of rice husks into multiple groups of test data according to preset rules; performing fermentation tests on multiple groups of test data according to the same preparation environment factors in accordance with the organic auxiliary material preparation method to obtain multiple groups of fermentation monitoring information; analyzing and judging whether to ferment according to multiple groups of fermentation monitoring information, and determining the complete fermentation time during fermentation to obtain the optimal fermentation time of the test; performing a two-factor correlation analysis on the optimal fermentation time of the test in combination with the weight and average particle size of rice husks to obtain a multi-factor correlation relationship, so that when the organic auxiliary materials are prepared for composting fermentation, the target fermentation time is determined according to the weight and average particle size of the rice husks, and then the organic auxiliary materials are obtained after the target fermentation time is reached to complete the preparation of the organic auxiliary materials. Among them, the preparation environment factors are kept consistent with the environmental factors of the actual prepared organic auxiliary materials.

[0068] Furthermore, when a two-factor correlation analysis is performed on the experimental optimal fermentation time in combination with the weight and average particle size of the rice husk, the distribution information of the rice husk particle size is obtained, the distribution of the rice husk particle size is determined, and the rice husk particle size distribution is used as a single variable for screening in multiple groups of experimental data to obtain a target screening result, and a correlation analysis is performed on the target screening result in combination with the experimental optimal fermentation time corresponding to the experimental data in the target screening result, and then when the target fermentation time is determined according to the weight and average particle size of the rice husk, the particle size distribution of the rice husk is obtained, and a correction factor is determined based on the particle size distribution and the correlation relationship, and then the correction factor is used to correct the target fermentation time to obtain the corrected target fermentation time, and then the organic excipient is obtained according to the corrected target fermentation time to complete the preparation of the organic excipient.

[0069] The above technical solution can better perform composting fermentation when the organic auxiliary materials are prepared by analyzing the correlation between rice husks and fermentation time in advance, so that the preparation of organic auxiliary materials can be measured based on the target fermentation time, which provides convenience for the preparation of organic auxiliary materials, makes full use of the materials for composting fermentation, avoids the waste of materials due to insufficient composting fermentation, improves the preparation rate of organic auxiliary materials, and can also ensure the quality of organic auxiliary materials, avoids the problem of quality control of organic auxiliary materials caused by insufficient composting fermentation, and affects the preparation of microbial organic fertilizer. In addition, by using the correction factor to correct the target fermentation time, the influence of different rice husk particle sizes on composting fermentation is fully considered, and the accuracy of the target fermentation time is improved, so that the corrected target fermentation time can be used as a reference when preparing organic auxiliary materials, thereby improving the preparation efficiency of organic auxiliary materials, providing guarantee for the preparation of organic auxiliary materials, and then guaranteeing the preparation of microbial organic fertilizer.

[0070] Comparative Example 1

[0071] The difference from Example 1 is that the modified fullerene C60 is missing in equal parts by weight, and the other parts are the same.

[0072] Comparative Example 2

[0073] The difference from Example 1 is that the step of modifying fullerene C60 is omitted, and fullerene C60 of equal weight parts is directly used, and the rest are the same.

[0074] Comparative Example 3

[0075] The difference from Example 1 is that distiller's grains are used instead of bean dregs when preparing the dry bean dregs enzymatic hydrolysate, and the other aspects are the same.

[0076] Comparative Example 4

[0077] The difference from Example 1 is that Phanerochaete chrysosporium is missing, and the others are the same.

[0078] Comparative Example 5

[0079] The difference from Example 1 is the lack of Lactobacillus plantarum, and the others are the same.

[0080] Performance Testing

[0081] In the microbial organic fertilizer containing fullerene C60 prepared in Example 1, the number of effective live bacteria is greater than 20 million / g, the mass fraction of organic matter (on a drying basis) is 56.5%, the mass fraction of total nutrients (nitrogen + phosphorus pentoxide + potassium oxide) is 8.3%, the mass fraction of water (fresh sample) is 22%, the mortality rate of ascaris eggs reaches 100%, the number of fecal coliform bacteria is less than 3 / g, total arsenic, mercury, lead, cadmium and chromium are not detected, and the pH is 6.9, which has reached the relevant national standards NY 525-2012 and NY 884-2012 for organic fertilizers.

[0082] The soil improvement effect test and the crop growth and yield test were carried out on Example 1 and the comparative example. The results are shown in Table 1. The soil pH value (average value) of the saline-alkali land test field is 8.5, the soil salt content (g / kg) is 4.2, the soil organic matter content (%) is 1.0, the sorghum model is Yajin No. 6, and it is planted in early May. The row spacing during row sowing is 50 cm and the tillage thickness is 20 cm.

[0083] Table 1

[0084]

[0085] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. If these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A microbial organic fertilizer containing fullerene C60, the raw materials for preparation include biologically improved composite bacterial liquid, modified fullerene C60 and organic auxiliary materials.

2. The microbial organic fertilizer containing fullerene C60 according to claim 1, characterized in that, The preparation raw materials include 1-5 parts of biologically improved composite bacterial liquid, 8-15 parts of modified fullerene C60 and 15-30 parts of organic auxiliary materials by weight.

3. The preparation method of the microbial organic fertilizer containing fullerene C60 according to claim 1 or 2, characterized in that, include: By weight, 1-5 parts of the prepared biologically improved composite bacterial liquid, 8-15 parts of modified fullerene C60 and 15-30 parts of organic auxiliary materials are mixed.

4. The method for preparing the microbial organic fertilizer containing fullerene C60 according to claim 3, characterized in that, The preparation method of the modified fullerene C60 comprises: mixing 40-50 parts of ethylenediamine, 100-120 parts of ethanol and 20-50 parts of water by weight to obtain a solution A, respectively weighing 0.2-0.5 parts of fullerene C60 and 2-5 parts of NaOH and adding them to 200-250 parts of toluene to obtain a solution B, stirring 20-25 parts of the solution A and 2-5 parts of the solution B under nitrogen protection for reaction, standing to separate the liquids, taking out the lower layer of liquid by suction filtration, washing, and vacuum drying to obtain the modified fullerene C60; Preferably, the reaction time under nitrogen protection is 7-10 days, and the stirring speed is 120-150r / min; Preferably, the standing time is 24-30h; Preferably, washing with 95% ethanol; Preferably, the vacuum drying temperature is 60-80°C and the time is 48-55h.

5. The method for preparing the microbial organic fertilizer containing fullerene C60 according to claim 3, characterized in that, The preparation method of the biologically improved composite bacterial liquid comprises: uniformly mixing methylotrophic bacillus fermentation liquid, polymyxa bacillus fermentation liquid, cerevisiae fermentation liquid, rhodopseudomonas fermentation liquid, Phanerochaete chrysosporium fermentation liquid, Lactobacillus plantarum fermentation liquid and Aspergillus niger fermentation liquid in a volume ratio of 1.5:1.5:1.5:1:1:0.5:5 to obtain the biologically improved composite bacterial liquid.

6. The method for preparing the microbial organic fertilizer containing fullerene C60 according to claim 3, characterized in that, The preparation method of the organic auxiliary material comprises: mixing fresh chicken manure, dry bean dregs and rice husks, wherein the weight ratio of fresh chicken manure to rice husks is 5:4, adding dry bean dregs with a weight of 5% of the weight of fresh chicken manure, adding a biologically improved composite bacterial liquid with a weight of 3% of the weight of fresh chicken manure, composting and fermenting to obtain the organic auxiliary material.

7. The method for preparing the microbial organic fertilizer containing fullerene C60 according to claim 6, characterized in that: The rice husk has a particle size of 60-80 meshes and a fermentation time of 20-25 days to prepare an organic auxiliary material.

8. The method for preparing the microbial organic fertilizer containing fullerene C60 according to claim 5, characterized in that, The preparation methods of the methylotrophic bacillus fermentation liquid, the polymyxa bacillus fermentation liquid, the saccharomyces cerevisiae fermentation liquid, the rhodopseudomonas fermentation liquid, the Phanerochaete chrysosporium fermentation liquid and the lactobacillus plantarum fermentation liquid comprise: in a sterile environment, picking up activated single colonies, inoculating them into a liquid fermentation medium, and culturing them at a constant temperature to obtain the fermentation liquid; the preparation method of the Aspergillus niger fermentation liquid comprises: in a sterile environment, collecting activated Aspergillus niger spores with sterile water, inoculating them into a liquid fermentation medium, and culturing them at a constant temperature.

9. The method for preparing the microbial organic fertilizer containing fullerene C60 according to claim 8, characterized in that, The activation is carried out under a sterile environment, coating methylotrophic Bacillus, Paenibacillus polymyxa, Saccharomyces cerevisiae, Rhodopseudomonas, Phanerochaete chrysosporium, Lactobacillus plantarum and Aspergillus niger on a solid activation culture medium plate, and culturing at a constant temperature of 28-30° C. for 24-96 hours to obtain activated colonies and Aspergillus niger spores; Preferably, the liquid fermentation medium comprises dry bean dregs enzymatic hydrolyzate.

10. The method for preparing the microbial organic fertilizer containing fullerene C60 according to claim 9, characterized in that: The preparation method of the dry bean dregs enzymatic hydrolyzate comprises the following steps: uniformly mixing the dry bean dregs and tap water in a weight ratio of 1:20, adjusting the pH value to 9 with an ammonia solution, water bathing at 80° C. for 60 minutes, adding alkaline protease at a rate of 300 U / g-dry bean dregs for enzymatic hydrolysis at 50° C. for 24 hours, and centrifuging to obtain a dry bean dregs enzymatic hydrolyzate rich in amino acids with a concentration of 5%.

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