Application of 2-amino caprylic acid or derivative thereof or pharmaceutically acceptable salt thereof in prevention and treatment of plant diseases, promotion of plant growth and preparation

Abstract

The invention relates to the technical field of plant disease prevention and treatment, in particular to application of any one or more of 2-amino caprylic acid or derivatives thereof or pharmaceutically acceptable salts thereof: 1) application in prevention and treatment of plant diseases caused by phytopathogens; 2) application in preparation of preparations for preventing and treating plant diseases caused by phytopathogens; 3) application in promotion of plant root growth; 4) application in preparation of a preparation for promoting plant root growth; and 5) application in improving dry weight or fresh weight of plant roots and / or leaves. According to the invention, 2-aminooctanoic acid is applied to the roots of plants, so that the growth of fusarium solani can be inhibited, and the occurrence of panax notoginseng root rot can be slowed down; meanwhile, the invention further finds that the 2-aminooctanoic acid can promote the growth of beneficial microorganisms such as penicillium, mortierella and trichoderma, thereby promoting the growth of plant roots.

Description

Technical Field

[0001] The invention relates to the technical field of plant disease prevention and control, and in particular to the application of 2-aminocaprylic acid or its derivatives or pharmaceutically acceptable salts thereof in preventing and controlling plant diseases, promoting plant growth and preparing preparations. Background Art

[0002] 2-Aminocaprylic acid (CAS No. 2187-07-7), molecular formula: C8H17NO2, molecular weight: 159.226, comes from the root secretions of pine trees.

[0003] At present, the market demand for Panax notoginseng is increasing, and the scale of Panax notoginseng cultivation is expanding. However, it is found that the soil that has been planted with Panax notoginseng has the problem of continuous cropping obstacles, and Panax notoginseng can no longer be planted. These reasons have led to major problems such as lack of land for Panax notoginseng cultivation, which has restricted the development of the Panax notoginseng industry. Among them, the problem of continuous cropping obstacles of Panax notoginseng is extremely significant, and its main phenomena are: (1) the germination of Panax notoginseng is affected and thus reduced; (2) the quality and growth of Panax notoginseng plants are weakened; (3) when the root rot is serious, the yield of Panax notoginseng will be affected or even fail; (4) the land that has been planted with Panax notoginseng needs to be rotated, and the interval required may be long, and it may take 10 to 30 years of rotation before Panax notoginseng can be replanted. It is difficult to implement on a large scale in actual production; the application of chemical pesticides is only one of the means to temporarily alleviate plant diseases and insect pests, and the prevention and control effect is not stable. It will also cause serious pollution to the ecological environment and cannot solve the problem from the root, and may even lead to soil acidification and salinization. Soil fumigation and other treatment methods seriously affect the quality of medicinal materials, destroy soil structure, and inhibit the reproduction of microorganisms in the soil. Due to the increasingly high requirements for the quality of Panax notoginseng, the complexity of root rot and the limitations of various prevention and control measures, improving quality and reducing diseases have become urgent issues to be solved in Panax notoginseng production.

[0004] In the prior art, there is a lack of effective methods for promoting the growth of Panax notoginseng and preventing and treating Panax notoginseng root rot. In view of this, the present invention is specially proposed. Summary of the invention

[0005] In order to solve the above problems in the prior art, the present invention provides the following technical solutions: In a first aspect, the present invention provides any one or more uses of 2-aminocaprylic acid or its derivatives or pharmaceutically acceptable salts thereof: 1) Application in the prevention and treatment of plant diseases caused by plant pathogens; 2) Use in the preparation of preparations for preventing and controlling plant diseases caused by plant pathogens; 3) Application in promoting plant root growth; 4) Application in the preparation of preparations for promoting plant root growth; 5) Application in increasing the dry weight of plant roots and / or leaves.

[0006] The present invention finds that applying 2-aminocaprylic acid to the roots of plants can increase the survival rate of annual Panax notoginseng; at the same time, the present invention further finds that 2-aminocaprylic acid can also promote the growth of beneficial microorganisms such as Penicillium and Mortierella, thereby promoting the growth of plant roots.

[0007] Preferably, the plant pathogenic bacteria include Fusarium solani.

[0008] Preferably, the plant disease comprises root rot.

[0009] Preferably, the preparation for preventing and controlling plant diseases caused by plant pathogens comprises a bacteriostatic agent.

[0010] Preferably, the preparation for promoting plant root growth comprises a plant growth regulator.

[0011] Preferably, the plant belongs to the genus Panax; more preferably, the plant is Panax notoginseng.

[0012] In a second aspect, the present invention provides an antibacterial agent for plant pathogens, which contains 2-aminocaprylic acid or its derivatives or pharmaceutically acceptable salts thereof.

[0013] Preferably, the concentration of the 2-aminocaprylic acid or its derivative or its pharmaceutically acceptable salt in the antibacterial agent is higher than 0.01 ppm.

[0014] More preferably, the concentration of 2-aminocaprylic acid or its derivative or pharmaceutically acceptable salt thereof in the antibacterial agent is 0.01ppm~10ppm, for example, it can be any value among 0.01ppm, 0.05ppm, 0.1ppm, 0.15ppm, 0.2ppm, 0.25ppm, 0.50ppm, 1ppm, 2ppm, 3ppm, 4ppm, 5ppm, 6ppm, 7ppm, 8ppm, 9ppm and 10ppm, or, a numerical range in which any two of the above values ​​are endpoint values.

[0015] The present invention finds that when the concentration of 2-aminocaprylic acid is above 0.01ppm, the mycelial growth of Fusarium solani is significantly lower than that of the blank control group, indicating that the occurrence of Panax notoginseng root rot can be significantly inhibited when the concentration of 2-aminocaprylic acid is low; when the concentration of 2-aminocaprylic acid is increased to 10ppm, the mycelial growth of Fusarium solani is lower than that of the blank control group, and the mycelial growth cannot be extended outward. With the further increase of the concentration, the inhibitory effect does not increase significantly, indicating that the antibacterial effect of 2-aminocaprylic acid on Fusarium solani is not obvious when the concentration is high.

[0016] In a third aspect, the present invention provides a plant growth regulator, characterized in that it contains 2-aminocaprylic acid or its derivatives or pharmaceutically acceptable salts thereof.

[0017] Preferably, the concentration of the 2-aminocaprylic acid or its derivative or its pharmaceutically acceptable salt in the plant growth regulator is not less than 0.01 ppm.

[0018] More preferably, the concentration of the 2-aminocaprylic acid or its derivative or its pharmaceutically acceptable salt in the plant growth regulator is 0.01ppm~10ppm, for example, it can be any value among 0.01ppm, 0.05ppm, 0.1ppm, 0.15ppm, 0.2ppm, 0.25ppm, 0.50ppm, 1ppm, 2ppm, 3ppm, 4ppm, 5ppm, 6ppm, 7ppm, 8ppm, 9ppm and 10ppm, or a numerical range in which any two of the above values ​​are endpoint values.

[0019] The beneficial effects of the present invention are: 1) The 2-aminocaprylic acid or its derivatives or pharmaceutically acceptable salts described in the present invention have important uses in preventing and controlling root rot caused by Fusarium solani, especially can inhibit the growth of Fusarium solani, thereby effectively preventing and controlling root rot of Panax notoginseng, and provides a new control agent for preventing and controlling Panax notoginseng diseases.

[0020] 2) Fusarium solani has poor resistance to 2-aminocaprylic acid. At the same time, it can improve the survival rate of Panax notoginseng seedlings, increase the dry weight of the aboveground and underground parts of Panax notoginseng plants, and promote the growth of Panax notoginseng roots, which meets the requirements of sustainable development. It has broad research and market application prospects.

[0021] 3) The present invention provides an application of 2-aminooctanoic acid, a metabolite secreted from plant roots, in Panax notoginseng, which can not only prevent and treat Panax notoginseng root rot, but also increase the dry weight of Panax notoginseng. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0023] Figure 1 This is the inhibitory effect of 2-aminocaprylic acid on Fusarium solani in Example 1 of the present invention.

[0024] Figure 2 This is a photo of the drug-treated plate of 2-aminocaprylic acid against Fusarium solani in Example 1 of the present invention.

[0025] Figure 3 This is the growth-promoting effect of 2-aminocaprylic acid on Mortierella in Example 2 of the present invention.

[0026] Figure 4 This is a photo of the drug-treated plate of 2-aminocaprylic acid against Mortierella in Example 2 of the present invention.

[0027] Figure 5 This is the growth-promoting effect of 2-aminocaprylic acid on Penicillium in Example 3 of the present invention.

[0028] Figure 6 This is a photo of the drug-treated plate of 2-aminocaprylic acid against Penicillium in Example 3 of the present invention.

[0029] Figure 7 This is the effect of 2-aminocaprylic acid on the survival rate of Panax notoginseng seedlings in Example 4 of the present invention.

[0030] Figure 8 This is the effect of 2-aminocaprylic acid on the aboveground dry weight of Panax notoginseng individual plants in Example 4 of the present invention.

[0031] Fig. 9 This is a diagram showing the effect of 2-aminocaprylic acid on the dry weight of individual underground plants of Panax notoginseng potted plants in Example 4 of the present invention.

[0032] Fig.10 This is a diagram showing the effect of 2-aminocaprylic acid on the root length of Panax notoginseng in Example 4 of the present invention.

[0033] Fig.11 This is a diagram showing the effect of 2-aminocaprylic acid on the root surface area of ​​Panax notoginseng root fibers in Example 4 of the present invention.

[0034] Fig.12 This is a drug-carrying plate of palmitic acid against Fusarium solani provided in Comparative Example 1 of the present invention.

[0035] Fig.13 This is a diagram showing the inhibitory effect of palmitic acid on Fusarium solani provided in Comparative Example 1 of the present invention.

[0036] Fig.14 This is the effect of palmitic acid on the survival rate of Panax notoginseng provided in Comparative Example 2 of the present invention.

[0037] Fig.15 This is the effect of palmitic acid on the aboveground dry weight of a single Panax notoginseng plant provided in Comparative Example 2 of the present invention.

[0038] Fig.16 This is the effect of palmitic acid on the dry weight of a single underground Panax notoginseng plant provided in Comparative Example 2 of the present invention.

[0039] Fig.17This is the effect of N-methyl-DL-alanine provided in Comparative Example 2 of the present invention on the dry weight of a single plant of Panax notoginseng above ground.

[0040] Fig.18 This is the effect of N-methyl-DL-alanine provided in Comparative Example 2 of the present invention on the dry weight of individual underground Panax notoginseng plants. DETAILED DESCRIPTION

[0041] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with the drawings of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0042] Unless otherwise specified, the various raw materials used in the examples and comparative examples are commercially available conventional raw materials, and the technical means used are conventional means well known to those skilled in the art.

[0043] Example 1 This example verifies the inhibitory effect of 2-aminocaprylic acid on Fusarium solani. The specific experimental steps are as follows: 1. Place 200 ml of PDA culture medium (40 g of potato, 3 g of agar, 4 g of glucose, and 200 ml of water) in a high-pressure steam sterilizer and sterilize for 15 min at 121°C. After sterilization, place it in a clean bench for UV sterilization for 20 min.

[0044] 2. Prepare the compound into a solution: dissolve 10 mg of 2-aminooctanoic acid in 10 ml of methanol solution to prepare mother solution ①; Take 1ml① and add 9ml methanol→②; Take 1ml② and add 9ml methanol→③; Take 1ml③ and add 9ml methanol→④; The control is 10 ml methanol →⑤; 3. After sterilization, use a pipette to take 1 ml of each of ④-① and add them to 99 ml of culture medium to obtain culture medium with concentrations of 0.01ppm, 0.1ppm, 1ppm and 10ppm, respectively. For the control group, take 1 ml of methanol solution and add it to 100 ml of culture medium. Shake well and pour into a plate, and cool. Use a 5 mm diameter puncher to punch the Fusarium solani into a cake of about 5 mm in size, and use an inoculation needle to inoculate the cake into the culture medium containing the compound solution, place it in an incubator at 26-28℃ for 5 days, observe the colony morphology and measure the colony diameter after 5 days.

[0045] like Figure 1 , Figure 2As shown, the results showed that when the concentration of 2-aminocaprylic acid was 0.01ppm, the mycelial growth of Fusarium solani was significantly lower than that of the control group, and the mycelium could not grow outward, indicating that 2-aminocaprylic acid at a concentration of 0.01ppm had a significant inhibitory effect on Fusarium solani; however, as the concentration increased to above 10ppm, the antibacterial effect did not increase but weakened, so the use range of 2-aminocaprylic acid is 0.01ppm-10ppm.

[0046] Example 2 This example verifies the growth-promoting effect of 2-aminocaprylic acid on Mortierella by bottle culture experiments. The specific experimental steps are: 1. Prepare the compound into a solution: dissolve 10 mg of 2-aminooctanoic acid in 10 ml of methanol solution to prepare mother solution ①; Take 1ml① and add 9ml methanol→②; Take 1ml② and add 9ml methanol→③; Take 1ml③ and add 9ml methanol→④; The control is 10 ml methanol →⑤; 2. After sterilization, use a pipette to take 1 ml of each of ④-① and add them to 99 ml of culture medium to obtain culture medium with concentrations of 0.01ppm, 0.1ppm, 1ppm and 10ppm, respectively. For the control group, take 1 ml of methanol solution and add it to 100 ml of culture medium. Shake well and pour on a plate, and cool. Use a 5 mm diameter puncher to punch the fungus into a cake of about 5 mm in size, and use an inoculation needle to inoculate the cake into the culture medium containing the compound solution, and place it in an incubator at 26-28℃ for 7 days. After 7 days, observe the colony morphology and measure the colony diameter.

[0047] like Figure 3 , Figure 4 As shown, the results showed that when the concentration of 2-aminocaprylic acid was 0.01ppm, the mycelium growth of Mortierella was slightly higher than that of the control group. When the concentration of 2-aminocaprylic acid was 0.1ppm, the mycelium growth of Mortierella was significantly higher than that of the control group, indicating that 2-aminocaprylic acid at a concentration of 0.1ppm has a significant growth-promoting effect on the mycelium of Mortierella; and with the increase of concentration, the growth-promoting effect is enhanced, so the use range of 2-aminocaprylic acid is 0.1ppm-10ppm.

[0048] Example 3 This example verifies the growth-promoting effect of 2-aminocaprylic acid on Penicillium through a bottle culture experiment. The specific experimental steps are: 1. Prepare the compound into a solution: dissolve 10 mg of 2-aminooctanoic acid in 10 ml of methanol solution to prepare mother solution ①; Take 1ml① and add 9ml methanol→②; Take 1ml② and add 9ml methanol→③; Take 1ml③ and add 9ml methanol→④; The control is 10 ml methanol →⑤; 2. After sterilization, use a pipette to take 1 ml of each of ④-① and add them to 99 ml of culture medium to obtain culture medium with concentrations of 0.01ppm, 0.1ppm, 1ppm and 10ppm respectively. For the control group, take 1 ml of methanol solution and add it to 100 ml of culture medium. Shake well and pour into a plate and cool. Dilute the cultured Penicillium with sterile water to a spore suspension concentration of 104CFU / mL, use a pipette to take 50ul to the plate, use a coating rod to evenly spread the bacterial solution on the plate until there is no water on the surface of the plate, and then place the plate in an incubator at 26-28℃ for 2 days. After 2 days, observe the colony morphology and count the number of colonies.

[0049] like Figure 5 , Figure 6 As shown, the results showed that when the concentration of 2-aminocaprylic acid was 0.01ppm, the number of Penicillium was significantly higher than that of the control group. When the concentration of 2-aminocaprylic acid continued to increase to more than 0.1ppm, the promoting effect gradually weakened, indicating that 2-aminocaprylic acid has a significant growth-promoting effect on Penicillium; and with the increase of concentration, the growth-promoting effect first increased and then weakened, so the use range of 2-aminocaprylic acid is 0.01ppm-10ppm.

[0050] Example 4 This example verifies the effect of 2-aminocaprylic acid on improving the survival rate, dry weight per plant and root growth of annual Panax notoginseng. The specific experimental steps are: 1. Prepare the compound into a solution: Combined with the plate effect, the concentration of 2-aminocaprylic acid was selected as 0.1ppm in the Panax notoginseng potted plant experiment. 1mg of 2-aminocaprylic acid was added into 10ml of methanol to prepare the mother solution.

[0051] 2. Take 1 ml of the mother liquor and add it to 1 L of water with a concentration of 0.1 ppm. After mixing, add 50 ml of the compound to each pot according to the concentration. For the control, take 1 ml of methanol and add it to 1 L of water. Then the root irrigation experiment continued for 6 weeks (once a week). After the Panax notoginseng continued to grow for two months, the potted Panax notoginseng was sampled, cleaned, and the number of Panax notoginseng plants treated with each compound concentration gradient was counted. The Panax notoginseng fibrous roots were scanned, and the above-ground and underground parts were put into envelopes respectively, sterilized at 115℃ for 30 minutes, dried at 60℃ for 3 days, weighed and calculated.

[0052] Figure 7 The results showed that root irrigation of 2-aminocaprylic acid at a concentration of 0.1 ppm can significantly increase the survival rate of Panax notoginseng seedlings, indicating that 2-aminocaprylic acid is beneficial to the growth of Panax notoginseng. Figure 8 and Fig. 9 The results showed that root irrigation with 2-aminocaprylic acid at a concentration of 0.1 ppm could significantly increase the dry weight of the aboveground and underground parts of each plant. Fig.10 and Fig.11 Root irrigation of 2-aminocaprylic acid at a concentration of 0.1ppm can significantly increase the growth of Panax notoginseng fibrous roots. The comprehensive results show that 0.1ppm can significantly improve the growth of Panax notoginseng plants.

[0053] Comparative Example 1 This comparative example provides another substance detected in root exudates - palmitic acid (CAS: 10025-99-7), and tests its inhibitory effect on Fusarium solani. The specific experimental steps are only different from those in Example 1 in that 2-aminooctanoic acid is replaced by palmitic acid.

[0054] The results of the effects of palmitic acid on Fusarium solani are as follows Fig.12 , Fig.13 As shown, after adding palmitic acid, at concentrations above 0.01 ppm, the diameter of the bacterial cake on the plate was significantly higher than that of the control. The results showed that palmitic acid promoted the growth of Fusarium solani at concentrations of 0.01 ppm and above.

[0055] Effect of palmitic acid on the survival rate of Panax notoginseng seedlings Fig.14 As shown in Figure 2, the effect of palmitic acid on the dry weight of Panax notoginseng aboveground plants is as follows: Fig.15 As shown in Figure 2, the effect of palmitic acid on the dry weight of Panax notoginseng underground plants is as follows: Fig.16 As shown, the results showed that root irrigating with palmitic acid at a concentration of 0.1 ppm would inhibit the growth of Panax notoginseng.

[0056] Comparative Example 2 This comparative example verifies the effect of another substance detected in root exudates, N-methyl-DL-alanine (CAS: 600-21-5), on the growth of Panax notoginseng potted plants through a bottle culture experiment. The specific experimental steps are only different from those in Example 2 in that 2-aminooctanoic acid is replaced with N-methyl-DL-alanine.

[0057] The results are as follows Fig.17 and Fig.18 As shown, the results showed that N-methyl-DL-alanine could not increase the dry weight of the aboveground and underground parts of Panax notoginseng.

[0058] In addition to the compounds listed in the above comparative examples, the present invention also verified other numerous compounds obtained from pine root secretions, and found that many other compounds could not simultaneously inhibit Fusarium solani and promote the growth of Panax notoginseng, and they will not be listed one by one here.

[0059] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. Application of any one or more of 2-aminocaprylic acid or its derivatives or pharmaceutically acceptable salts thereof: 1) Application in the prevention and treatment of plant diseases caused by plant pathogens; 2) Use in the preparation of preparations for preventing and controlling plant diseases caused by plant pathogens; 3) Application in promoting plant root growth; 4) Application in the preparation of preparations for promoting plant root growth; 5) Application in increasing the dry weight of plant roots and / or leaves.

2. The use of any one or more of 2-aminocaprylic acid or its derivatives or pharmaceutically acceptable salts thereof according to claim 1, characterized in that: The plant pathogenic bacteria include Fusarium solani.

3. The use of any one or more of 2-aminocaprylic acid or its derivatives or pharmaceutically acceptable salts thereof according to claim 1 or 2, characterized in that: The plant diseases include root rot.

4. The use of any one or more of 2-aminocaprylic acid or its derivatives or pharmaceutically acceptable salts thereof according to any one of claims 1 to 3, characterized in that: The preparation for preventing and controlling plant diseases caused by plant pathogenic bacteria includes a bacteriostatic agent.

5. The use of any one or more of 2-aminocaprylic acid or its derivatives or pharmaceutically acceptable salts thereof according to any one of claims 1 to 4, characterized in that: The preparation for promoting plant root growth includes plant growth regulators.

6. The use of any one or more of 2-aminocaprylic acid or its derivatives or pharmaceutically acceptable salts thereof according to any one of claims 1 to 5, characterized in that: The plant belongs to the genus Panax; preferably, the plant is Panax notoginseng.

7. A bacteriostatic agent for plant pathogens, characterized in that: Contains 2-aminocaprylic acid or its derivatives or pharmaceutically acceptable salts thereof.

8. The antibacterial agent for plant pathogens according to claim 7, characterized in that: The concentration of 2-aminocaprylic acid or its derivative or its pharmaceutically acceptable salt in the antibacterial agent is higher than 0.01 ppm; Preferably, the concentration of the 2-aminocaprylic acid or its derivative or its pharmaceutically acceptable salt in the antibacterial agent is 0.01 ppm to 10 ppm.

9. A plant growth regulator, characterized in that Contains 2-aminocaprylic acid or its derivatives or pharmaceutically acceptable salts thereof.

10. The plant growth regulator according to claim 9, characterized in that The concentration of the 2-aminocaprylic acid or its derivative or its pharmaceutically acceptable salt in the plant growth regulator is not less than 0.01 ppm; more preferably 0.01 ppm to 10 ppm.

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