Bactericidal composition containing WML-01 and triazole compound as well as preparation and application thereof

Abstract

The invention discloses a bactericidal composition containing WML-01 and a triazole compound, and is characterized in that the bactericidal composition is composed of the WML-01 and the triazole compound, and the mass ratio of the WML-01 to the triazole compound is (10: 1)-(1: 10). The bactericidal composition taking the WML-01 and the triazole medicament as effective components has a synergistic effect on prevention and treatment of rice bakanae disease, wheat stem rot, wheat scab, plant root rot and sclerotinia rot of colza, can effectively improve the disease prevention and treatment effect, reduces the pesticide consumption, delays the occurrence of drug resistance, improves the crop yield, and has a good application prospect. The method is safe, effective and economical, and has a wide market application prospect.

Description

Technical Field

[0001] The present invention relates to the technical field of pesticides, and in particular to a fungicide composition containing WML-01 and a triazole compound, and a preparation and application thereof. Background Art

[0002] Rice bakanae disease, also known as leggy growth disease, is an important disease of rice production and occurs in major rice-producing areas in my country. The main pathogen is Fusarium fujikura ( Fusarium fujikuroi ), infected seeds and diseased debris in the field are the main initial sources of infection for rice seedling rot. Diseased grains often do not germinate or cannot emerge after sowing. Normally, diseased seedlings that become ill during the seedling stage are thinner and taller than healthy seedlings, with slender leaves and sheaths, pale yellow leaves, poorly developed root systems, and some diseased seedlings die before transplanting. After the infected rice seedlings are planted, the mycelium can spread to the entire plant under suitable conditions, stimulating the excessive growth of stems and leaves. During the flowering period, the pathogen spreads to the floral organs, invading the glumes and endosperm, causing barren or deformed grains. If the pathogen invades late, although the grains do not show symptoms, the mycelium has already invaded the interior, making the seeds infected, which in turn affects the planting of the next crop of rice and causes great harm. Chemical control is still the main means of controlling rice seedling rot. Currently, the fungicides registered on the market for the control of rice seedling rot include carbendazim, prochloraz, fenpropimorph, and fludioxonil. However, due to the long-term, large-scale and frequent use of chemical agents, pathogens have gradually developed resistance to different fungicides, resulting in a significant reduction in the effectiveness of the agents. Increasing the amount of drugs used will bring about problems such as pesticide residues, seed safety and environmental pollution.

[0003] Wheat stem rot, commonly known as "crown rot" or "dryland foot disease", is caused by the fungus Fusarium graminearum ( Fusarium pseudograminearum ), Fusarium graminearum ( Fusarium graminearum ) is a global wheat stem base disease caused by various Fusarium species, including Fusarium graminearum. In my country's major wheat-producing areas, Fusarium graminearum is the dominant pathogen. In its early stages, wheat stem base rot can cause browning and rot in the young roots or coleoptiles, leading to seedling death in severe cases. The disease then gradually spreads to the first and second nodes of the wheat stem, causing the affected area to turn dark brown or rot, the leaf sheaths at the stem base to dry out, and the stem to turn brown and necrotic. In later stages, the browning of the stem base hinders the transport of water from the roots to the ear, resulting in a completely white ear. Once the ear is white, the grains cannot continue to develop, significantly impacting wheat seed set and yield. Currently, only eight pesticides have been registered for the control of this disease: fludioxonil, tebuconazole, difenoconazole, prothioconazole, trifluoperazine, and thiamethoxam. New pesticides are urgently needed for the prevention and control of this disease.

[0004] Wheat head blight, commonly known as "rotten wheat", is one of the world's wheat diseases, caused by infection with a variety of Fusarium graminearum, among which Fusarium graminearum (F. graminearum ) is the dominant pathogen in many wheat production areas. Wheat scab occurs in all regions of China, causing seedling blight, stem rot and ear rot. In epidemic years, the disease incidence of ears often reaches more than 50%. Not only the yield is greatly reduced, but also the quality is deteriorated. In addition, the diseased kernels contain toxins, which can cause poisoning in humans and animals after eating. The fungicides used to control wheat scab in production mainly include benzimidazole fungicides, triazole fungicides and strobilurin fungicides. The long-term and large-scale use of chemical fungicides has led to the increasingly prominent problem of resistance of wheat scab fungus.

[0005] Root rot is a common disease in plant production. The pathogen mainly damages the roots of plants, causing poor plant growth, seed rot, root rot, dwarfing, yellowing, and even whole plant death, resulting in serious yield loss. Soybean root rot is a major root disease in soybean production. It affects seedling growth and even causes seedling death during the seedling stage, reducing the number of seedlings in the field. During the adult stage, the growth and number of root nodules are affected due to root damage, leading to poor growth of the aboveground part, dwarfing, and affecting pod number and grain weight, thereby resulting in yield reduction. Currently, the fungicides registered for the control of soybean root rot on the market include difenoconazole, prochloraz, fludioxonil, carbendazim, and thiram.

[0006] Sclerotinia stem rot of rapeseed is the most serious disease that threatens rapeseed production. The pathogen is Sclerotinia sclerotiorum (Lib.) de Bary. Sclerotinia sclerotiorum The disease can infect stems, leaves, flowers, and silique of rapeseed, with the most severe damage on stems. After infection, water-stained, light brown lesions appear on the stems, which turn white in the middle and brown at the edge in the later stage. The lesion boundary is clear, and white fluffy mycelium grows on the lesion under humid conditions, eventually leading to stem rot, breakage, and whole plant death. Currently, there are very few pesticide varieties for the control of Sclerotinia stem rot of rapeseed, and most of them are concentrated in carbendazim, procymidone, and fludioxonil. The resistance of Sclerotinia sclerotiorum to carbendazim has already been serious. According to the 2024 national agricultural technology center monitoring report, strains isolated from rapeseed samples collected from 23 counties (cities, districts) in 8 provinces (municipalities) in Jiangsu and other provinces showed high resistance to carbendazim, with the frequency of resistant strains in Hunan, Jiangsu, Jiangxi, and Anhui provinces exceeding 98%. There is an urgent need for new pesticide varieties to control this disease and replace carbendazim to control the occurrence of drug resistance.

[0007] Triazole fungicides are the most widely used and the most varieties of fungicides in agriculture at present. The mechanism of action is to inhibit CYP51 enzyme (sterol 14α-demethylase) in pathogenic fungi, block ergosterol biosynthesis, and destroy the structure and function of fungal cell membrane. Triazole fungicides have protective, curative and eradicative effects. They have a broad spectrum of activity, high activity and good systemicity, and are widely used in the prevention and treatment of powdery mildew, rust, sclerotinia, sheath blight, leaf spot and root rot of cereals, fruits, vegetables and economic crops. The main varieties of triazole fungicides include triadimefon, triadimenol, tebuconazole, propiconazole, prothioconazole and fluquinconazole. Tebuconazole is a representative variety among them. The target of triazole fungicides is single, and long-term single use has the risk of inducing resistance. In addition, some triazole fungicides can affect the synthesis of gibberellin (GA) in crops, interfere with the metabolism of abscisic acid (ABA), and have a regulatory effect on crop growth. Therefore, controlling the use of these fungicides to avoid phytotoxicity is also a difficulty in the popularization and use of these fungicides.

[0008] At present, there are few fungicide varieties for the prevention and treatment of rice seedling blight, wheat foot rot, wheat scab, plant root rot and sclerotinia of rapeseed. With the long-term use of selective fungicides with single mechanism of action, the pathogenic fungi have developed resistance to varying degrees. It is of great significance to develop new fungicides with novel mechanism of action, to put them on the market, and to reasonably compound them with broad-spectrum and high-efficiency triazole fungicides to reduce the dosage of fungicides, improve the efficacy, delay the occurrence of resistance, and control the occurrence of diseases and prolong the life cycle of fungicides. SUMMARY

[0009] The purpose of the present application is to provide a fungicidal composition containing WML-01 and a triazole compound, as well as its preparation and application, in order to solve the technical problems of the current lack of fungicide varieties for the prevention and treatment of rice seedling blight, wheat foot rot, wheat scab, plant root rot and sclerotinia of rapeseed, and the development of resistance of pathogenic fungi to varying degrees with the long-term use of selective fungicides with single mechanism of action.

[0010] In order to achieve the purpose of the present application, the first aspect of the present application provides a fungicidal composition containing WML-01 and a triazole compound, which consists of WML-01 and tebuconazole, and the mass ratio of WML-01 to tebuconazole is 10:1 to 1:10. The WML-01 is a butenolide compound containing a thiazolidine structure, and its structural formula is shown in formula (I): .

[0011] In an alternative embodiment, the mass ratio of WML-01 to tebuconazole is 1:1 to 1:10.

[0012] In an alternative embodiment, the mass ratio of WML-01 to difenoconazole is 10:1 to 2:1.

[0013] A second aspect of the present application is to provide a fungicidal preparation comprising the fungicidal composition.

[0014] In an alternative embodiment, the fungicidal preparation is in the form of seed coating, suspension, emulsion, microemulsion, emulsifiable concentrate, microcapsule suspension preparation, nano-preparation, granule, wettable powder or water dispersible granule.

[0015] A third aspect of the present application is to provide an application of the fungicidal composition or the fungicidal preparation in the prevention of plant diseases, wherein the plant disease is one or more of rice seedling blight, wheat foot rot, wheat scab, soybean root rot, pepper anthracnose, Sphaeropsis sapinea, Sclerotinia sclerotiorum or cabbage Sclerotinia rot. Fusarium fujikuroi Fusarium pseudograminearum Fusarium graminearum Fusarium spp .、 Colletotrichum spp . Sclerotinia sclerotiorum

[0016] In an alternative embodiment, the components of the fungicidal preparation further comprise an auxiliary agent or carrier for assisting the fungicidal composition, wherein the auxiliary agent is xanthan gum, wetting agent, dispersing agent, defoaming agent, antifreezing agent or warning color.

[0017] In an alternative embodiment, the wetting agent is nonylphenol polyoxyethylene ether NP-10, alkylphenol polyoxyethylene ether OP-10 or alkyl naphthalene sulfonate EFW; the dispersing agent is sodium salt of alkyl naphthalene sulfonate condensate D-425 or sulfonate salt of alkyl naphthalene sulfonate condensate D-450; the defoaming agent is n-octanol or silicone SAG1522; the antifreezing agent is ethylene glycol; and the warning color is basic roseine, golden red or fast peach red.

[0018] Compared with the prior art, the present application has the following beneficial effects: The fungicidal composition provided by the present application has synergistic effect in the prevention of rice seedling blight, wheat foot rot, wheat scab, soybean root rot and cabbage Sclerotinia rot, can effectively improve the disease prevention effect, reduce the amount of pesticide used, delay the occurrence of drug resistance, improve crop yield, and is safe, effective and economical, thus having a broad market application prospect. DETAILED DESCRIPTION

[0019] ​​​​​​The following examples are intended to illustrate the present application but not to limit the scope of the present application. If not specified specifically, the technical means used in the examples are the conventional means well known to those skilled in the art, and the raw materials used are all commercially available.

[0020] The fungicidal composition and its preparation can be applied in various ways, and the present application is not particularly limited. One or a combination of seed dressing, coating, seed soaking, spraying, root irrigation, spreading, hole application, trench application, etc. can be used. According to the crop cultivation, the preparation form and the control object, the fungicidal composition is preferably seed coating, seed dressing and seed soaking treatment.

[0021] Example 1 In vitro fungistatic activity of WML-01 and difenoconazole after compounding on Pyricularia oryzae, Rhizoctonia cerealis, Fusarium graminearum, Diaporthe phaseolorum, Colletotrichum gloeosporioides and Sclerotinia sclerotiorum 1 Test materials 1.1 Test strains Pyricularia oryzae (provided by the Plant Protection College of China Agricultural University) was identified by conventional methods. F. fujikuroi

[0022] Diaporthe phaseolorum (provided by the Plant Protection College of China Agricultural University) was identified by conventional methods. F. oxysporum

[0023] Rhizoctonia cerealis (provided by the Plant Protection College of China Agricultural University) was identified by conventional methods. F. pseudograminearum

[0024] Fusarium graminearum (provided by the Plant Protection College of China Agricultural University) was identified by conventional methods. F. graminearum

[0025] Sclerotinia sclerotiorum (provided by the Plant Protection College of China Agricultural University) was identified by conventional methods. S. sclerotiorum

[0026] Colletotrichum gloeosporioides (provided by the Plant Protection College of China Agricultural University) was identified by conventional methods.

[0027] 1.2 Test agents WML-01 (99% technical material) was provided by the School of Science of China Agricultural University. The compound and its preparation method are disclosed in patent publication CN 118724887 A, and the compound number is 3-1.

[0028] The structural formula is shown in formula (I):

[0029] ​​​​​Difenoconazole (95% TC), product of Shandong Joint Pesticide Co., Ltd.

[0030] The above pesticide TC was prepared into a mother liquor with a concentration of 10 mg / L by using dimethyl sulfoxide as a solvent, and the mother liquor was stored in a refrigerator at 4 ℃ for standby use. 4

[0031] 1.3 Test medium Potato dextrose agar (PDA): potato 200 g, glucose 18 g, agar powder 12 g, distilled water 1000 mL.

[0032] 2 Test method The test was carried out according to NY / T 1156.2-2006 Guidelines for Pesticide Indoor Biological Test Inhibition of pathogenic fungi mycelium growth test plate method. The main operation process of the plate method is as follows: 5 concentration gradients are set between 10% and 90% of the inhibition rate of the agent on the target fungus, then a puncher with a diameter of 6 mm is used to punch a fungus cake on the same circumference near the edge of the activated target fungus (to ensure that the fungus age of the same repeat test fungus is the same), and the fungus cake is inoculated into the center of the series of drug-containing medium plates under sterile conditions using an inoculation needle, with the mycelium facing down, and a plate without drug is set as a control, and placed in the dark for culture. Each treatment is repeated 3 times for determination. The colony diameters of each concentration treatment are recorded, and the mycelium growth inhibition rate is calculated. Then the inhibition rate is converted into a probability value (Y), and the concentration of the agent is converted into a logarithmic value with 10 as the base (X), and X-Y is made into a regression straight line, and the toxicity regression curve equation Y=a+bX of each agent on the target fungus, as well as the correlation coefficient r and the effective inhibition concentration EC 50 .

[0033] The joint toxicity of the compound preparation is expressed by the method of co-toxicity coefficient of Sun Yunpei: Theoretical toxicity index of compound preparation TTI =∑(the toxicity index of a certain drug × the percentage of the active ingredient of the drug in the mixture).

[0034]

[0035] CTC ≧120 is synergistic effect; CTC ≦ 80 is antagonistic effect; 80 CTC <120 is additive effect.

[0036] 3 Test results Table 1 In vitro antibacterial activity of WML-01 and difenoconazole after compounding on rice seedling blight fungus

[0037] ​The above results show that: WML-01 and difenoconazole in the range of 10:1~1:10 (w / w) complex, by the method of synergistic coefficient determination of the synergistic coefficient of 96.14~201.01 between the wheat stem rot fungus, performance and synergistic effect, and when the mass ratio of WML-01 and difenoconazole in the range of 1:1~1:10, the synergistic coefficient is greater than 120, showing synergistic effect, and the synergistic effect is significant, the highest can reach 173.51.

[0038] Table 2 WML-01 and difenoconazole after the in vitro antibacterial activity of wheat stem rot fungus

[0039] The above results show that: WML-01 and difenoconazole in the range of 10:1~1:10 (w / w) complex, by the method of synergistic coefficient determination of the synergistic coefficient of 96.14~201.01 between the wheat stem rot fungus, performance and synergistic effect, and when the mass ratio of WML-01 and difenoconazole in the range of 1:1~1:10, the synergistic coefficient is greater than 120, showing synergistic effect, and the synergistic effect is significant, the highest can reach 173.51.

[0040] Table 3 WML-01 and difenoconazole after the in vitro antibacterial activity of wheat stem rot fungus

[0041] The above results show that: WML-01 and difenoconazole in the range of 10:1~1:10 (w / w) complex, by the method of synergistic coefficient determination of the synergistic coefficient of 96.14~201.01 between the wheat stem rot fungus, performance and synergistic effect, and when the mass ratio of WML-01 and difenoconazole in the range of 1:1~1:10, the synergistic coefficient is greater than 120, showing synergistic effect, and the synergistic effect is significant, the highest can reach 173.51.

[0042] Table 4 WML-01 and difenoconazole after the in vitro antibacterial activity of soybean root rot fungus

[0043] The above results show that: WML-01 and difenoconazole in the range of 10:1~1:10 (w / w) complex, by the method of synergistic coefficient determination of the synergistic coefficient of 96.14~201.01 between the wheat stem rot fungus, performance and synergistic effect, and when the mass ratio of WML-01 and difenoconazole in the range of 1:1~1:10, the synergistic coefficient is greater than 120, showing synergistic effect, and the synergistic effect is significant, the highest can reach 173.51.

[0044] Table 5 In vitro antibacterial activity of WML-01 and difenoconazole after compounding against Sclerotinia sclerotiorum of rape

[0045] The above results show that: WML-01 and difenoconazole are compounded in a mass ratio of 10:1~1:10 (w / w), and the co-toxicity coefficients of WML-01 and difenoconazole against Sclerotinia sclerotiorum are determined by the co-toxicity coefficient method. The co-toxicity coefficients are between 83.43~189.06, showing additive and synergistic effects. When the mass ratio of WML-01 and difenoconazole is in the range of 10:1~2:1, the co-toxicity coefficients are all greater than 120, showing significant synergistic effects, and the highest can reach 189.06.

[0046] Table 6 In vitro antibacterial activity of WML-01 and difenoconazole after compounding against Colletotrichum capsici of pepper

[0047] The above results show that: WML-01 and difenoconazole are compounded in a mass ratio of 10:1~1:10 (w / w), and the co-toxicity coefficients of WML-01 and difenoconazole against Colletotrichum capsici are determined by the co-toxicity coefficient method. The co-toxicity coefficients are between 83.03~112.17, showing additive effects, and no antagonistic effects.

[0048] Example 2 Field control effect of WML-01 and difenoconazole compounding on wheat scab 1. Materials and methods 1.1 Test materials 15% WML-01·difenoconazole suspension concentrate, self-made, wherein WML-01 5%, difenoconazole 10%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, and the balance is water, % is mass percentage content, prepared by sand milling process; 10% WML-01 suspension concentrate, self-made, wherein WML-01 10%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, and the balance is water, % is mass percentage content, prepared by sand milling process; 40% difenoconazole suspension concentrate, product of Qingdao Audis Biotechnology Co., Ltd., Shandong Province.

[0049] 1.2 Test site profile The test was conducted in Fengtai County, Anhui Province, with clay loam soil, medium fertility, wheat variety Huaiyumi No. 1, and seed rate of 25 kg per mu.

[0050] 1.3 Test design Table 7 Test scheme of seed treatment on wheat scab

[0051] 1.4 Survey Methods and Calculations Random sampling was conducted at 5 points in each treatment area, with 100 ears at each point. The samples were graded based on the percentage of the withered ear area to the entire ear area, and the disease index and control effect were calculated.

[0052] Fusarium scab grading standards: Level 0, the whole ear is disease-free; Level 1: the dead ear area accounts for less than 1 / 4 of the whole ear area; Level 3: the dead ear area accounts for 1 / 4-1 / 2 of the whole ear area; Level 5: the dead ear area accounts for 1 / 2-3 / 4 of the whole ear area; Level 7: the dead ear area accounts for more than 3 / 4 of the whole ear area; Calculation method of drug efficacy: Disease index (%) = ∑(number of diseased plants (ears) at each level × representative value at each level) / (total number of diseased plants (ears) surveyed × highest representative value) × 100 Control effect (%) = (disease index of control area after drug application - disease index of treated area after drug application) / disease index of control area after drug application × 100 2 Test results Table 8 Control effect of chemical treatment on wheat scab

[0053] Note: * The values ​​are the average of 4 repetitions; ** The significance level of variance analysis was p = 0.05.

[0054] From the contents of the above table, we can see that after two applications, the high-dose 200 ml treatment of 15% WML-01·difenoconazole suspension (active ingredient 30 g) had an 89.46% control effect on wheat fusarium scab, which was significantly better than the single-dose treatment of difenoconazole (active ingredient 16 g) and the single-dose treatment of WML-01 (active ingredient 20 g); the low-dose 100 ml treatment (active ingredient 15 g) had an 85.54% control effect on wheat fusarium scab, which was also better than the single-dose treatments of WML-01 and difenoconazole.

[0055] The above results show that after WML-01 and difenoconazole are combined, they show excellent control effect on wheat fusarium rust at a dosage similar to that of difenoconazole alone. Compared with WML-01 alone, the amount of medicine used in the field is reduced while the control effect is still better.

[0056] Example 3 Field control effect of WML-01 and difenoconazole on soybean root rot 1 Materials and Methods 1.1 Test materials 15% WML-01·Difenoconazole benzene ether suspension, self-made, wherein WML-01 5%, difenoconazole 10%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, the balance is water, % is the mass percentage content, prepared by sand mill process; 10% WML-01 suspension, self-made, wherein WML-01 10%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, the balance is water, % is the mass percentage content, prepared by sand mill process; 3% difenoconazole suspension, produced by Shandong Joint Pesticide Industry Co., Ltd.

[0057] 1.2 Test point profile The test site is located in the modern agricultural demonstration park of Beian City. The soil type is black soil, the organic matter content is 5.6%, and the pH value is 6.6. Sowing on May 21, 2024, using 65 cm ridge three cultivation mode, 320,000 seedlings per mu, 48% special fertilizer for soybean, the fertilizer amount is 320 kg per mu (N:P:K=15:23:10), and the seedlings emerge on June 1. The soil moisture content is good. The previous crop is soybean. Rely on natural precipitation.

[0058] 1.3 Test design Table 9 Test scheme of seed treatment on plant root rot

[0059] 1.4 Investigation method and calculation Soybean root rot investigation Investigate once every 30 d and 60 d after the soybean seedlings emerge. Randomly dig more than 30 soybean plants per plot to investigate, record the total number of plants and the number of plants at each level, investigate the incidence of root rot, and calculate the control effect.

[0060] Disease classification: (root lesion area) 0 level: main root, fibrous root is healthy, no lesion, and nodule is more; 1 level: there are sporadic lesions on the main root, but not in pieces, and there are a few lesions on the fibrous root; 3 level: the main root lesion is scattered, but less than 1 / 4 of the root area, and the fibrous root is slightly diseased; 5 level: the main root lesion is between 1 / 4 and 1 / 2 of the root area, and the fibrous root is more diseased, but not in pieces; 7 level: the main root lesion is between 1 / 2 and 1 / 4 of the root area, and the fibrous root lesion is in pieces, and part of the fibrous root falls off; 9 level: the whole root is surrounded by lesions, the root is rotten, and the fibrous root is almost none.

[0061] Method for calculating efficacy: Disease index (%) =∑(number of diseased plants (ears) of each level × representative value of each level) / (total number of plants (ears) surveyed × representative value of the highest level) × 100 Control effect (%) = (disease index after treatment in the control area - disease index after treatment in the treatment area) / disease index after treatment in the control area × 100 2 Test results Table 10. Effects of fungicide treatment on soybean root rot

[0062] Note: * The values are the average of 4 replicates; ** The significance level of variance analysis is p = 0.05.

[0063] From the contents in the above table, it can be seen that 30 days after emergence, the 15% WML-01 · difenoconazole suspension concentrate at a high dose of 200 ml seed dressing treatment (effective ingredient 30 g) had a control effect of 87.52% on soybean root rot, which was significantly better than the WML-01 single agent (effective ingredient 20 g) and the difenoconazole single agent treatment (effective ingredient 12 g); the 15% WML-01 · difenoconazole suspension concentrate at a low dose of 100 ml treatment (effective ingredient 15 g) had a control effect of 80.94% on soybean root rot, which was significantly better than the difenoconazole single agent and the WML-01 single agent treatment.

[0064] 60 days after sowing, the 15% WML-01 · difenoconazole suspension concentrate at 100 ml and 200 ml seed dressing treatment had excellent control effects on soybean root rot, which were 87.90% and 93.30% respectively, and were significantly better than the WML-01 single agent and the difenoconazole single agent treatment.

[0065] The above results show that after the WML-01 and difenoconazole are compounded, at about the same dose as the difenoconazole single agent, excellent control effects on soybean root rot are shown, and relative to the WML-01 single agent treatment, the field use amount is reduced while the control effect is improved.

[0066] Example 4. Field control effect of WML-01 and difenoconazole compound on wheat foot rot 1. Materials and methods 1.1 Test materials 15% WML-01·Difenoconazole benzene ether suspension, self-made, wherein WML-01 5%, difenoconazole 10%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, the balance being water, % being mass percentage content, prepared by sand mill process; 10% WML-01 suspension, self-made, wherein WML-01 10%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, the balance being water, % being mass percentage content, prepared by sand mill process; 3% difenoconazole benzene ether suspension, produced by Shandong Lianhe Pesticide Co., Ltd.

[0067] 1.2 Test point profile The test was set in Feixiang District, Handan City, Hebei Province, the soil was clay loam, the fertility was medium, the wheat variety was Woyu No. 3, and the seed amount per mu was 20 kg.

[0068] 1.3 Test design Table 11 Test scheme of seed treatment on wheat stem base rot

[0069] 1.4 Investigation method and calculation Stem base rot investigation a) Investigation of prevention effect at the filling stage of stem base rot Randomly sample 5 points per treatment area, 100 plants per point, investigate the number of diseased plants and disease index, and calculate disease index and control effect.

[0070] Stem base rot grading standards: 0 level, plant not diseased; 1 level, stem obviously brown in the ground; 3 level, first internode brown; 5 level, second internode brown; 7 level, third internode appears brown symptoms, but no white head; 9 level, white head or plant death due to disease.

[0071] Pharmacodynamic calculation method: Disease index (%) = ∑ (number of plants (ears) of each level × representative value of each level) / (total number of plants (ears) investigated × representative value of highest level) × 100 Control effect (%) = (disease index after treatment in control area - disease index after treatment in treatment area) / disease index after treatment in control area × 100 b) Investigation of white head rate at the filling stage Randomly sample 5 points per treatment area, 100 plants per point, investigate total ear number and white ear number, and calculate control effect.

[0072] White head rate (%) = white ear number / total number of plants investigated × 100 Control effect (%) = [white tip rate of control area - white tip rate of treatment area] / white tip rate of control area x 100 2 Test results Table 12 Effect of the treatment on wheat stem base rot

[0073] Note: * The average value of 4 repetitions; ** The significance level of variance analysis is p = 0.05.

[0074] From the content in the above table, it can be seen that during the investigation of the grain-filling stage, the high-dose 200 ml treatment (effective ingredient 30 g) of 15% WML-01 · difenoconazole suspension agent on wheat stem base rot has an effect of 84.69%, which is obviously better than the single-dose treatment of difenoconazole (effective ingredient 12 g); the low-dose 100 ml treatment (effective ingredient 15 g) has an effect of 83.70% on wheat stem base rot, which is significantly better than the single-dose treatment of difenoconazole and slightly better than the single-dose treatment of WML-01 (effective ingredient 20 g).

[0075] The investigation results of white tip rate show that the high-dose 200 ml treatment of 15% WML-01 · difenoconazole suspension agent has an effect of 93.96% on white tip; the low-dose 100 ml treatment has an effect of 87.77% on white tip, which is significantly better than the single-dose treatment of WML-01 and difenoconazole.

[0076] The above results show that after the compounding of WML-01 and difenoconazole, the compound has an excellent effect on wheat stem base rot at the same dose as the single dose of difenoconazole, and compared with the single-dose treatment of WML-01, the field use amount is reduced while the effect is improved.

[0077] Example 5 Field effect of WML-01 and difenoconazole compound on rice sheath blight 1 Materials and methods 1.1 Test material 15% WML-01 · difenoconazole suspension agent is self-made, wherein WML-01 5%, difenoconazole 10%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, and the balance is water, % is the mass percentage content, and it is prepared by sand milling process; 10% WML-01 suspension agent is self-made, wherein WML-01 10%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, and the balance is water, % is the mass percentage content, and it is prepared by sand milling process; 3% difenoconazole suspension agent is produced by Shandong Joint Pesticide Industry Co., Ltd.

[0078] 1.2 Test point profile The test was set in Zhao Yuan Village, Yaojiang Town, Zhuji City, Zhejiang Province, with clay loam soil, medium fertility, rice variety Zhongzao 39, seed rate 5 kg per mu, seedling and transplanting.

[0079] 1.3 Test design Table 13 Test scheme of seed treatment on rice seedling blight

[0080] 1.4 Investigation method and calculation Seedling blight: randomly sample 5 points per plot in the seedbed, investigate 10 cm*10 cm per point, repeat 3 times, record the disease rate, and calculate the control effect.

[0081] Disease rate (%) = number of diseased plants / total number of plants investigated x 100% Control effect (%) = (disease rate of blank control area - disease rate of treatment area) / disease rate of blank control area x 100% 2 Test results Table 14 Control effect of seed treatment on rice seedling blight

[0082] Note: * 4 repeated average values; ** The significance level of variance analysis is p=0.05.

[0083] From the contents in the above table: during seedling stage, 15% WML-01·difenoconazole suspension concentrate high dose 200 ml seed treatment (effective ingredient 30 g), the control effect on rice seedling blight was 97.97%; 100 ml seed treatment (effective ingredient 15 g), the control effect on rice seedling blight reached 95.44%, which was significantly better than difenoconazole single agent treatment (15 g) and WML-01 single agent treatment (effective ingredient 20 g).

[0084] The above results show that after compounding WML-01 and difenoconazole, at the same dose as difenoconazole single agent, excellent control effect on seedling blight is shown, and compared with WML-01 single agent, the field drug dosage is significantly reduced and the control effect is also better.

[0085] Example 6 Field control effect of WML-01 and difenoconazole compound on sclerotinia stem rot of rape 1 Materials and methods 1.1 Test materials 15% WML-01·Difenoconazole benzene ether suspension agent, self-made, wherein WML-01 10%, difenoconazole 5%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, the balance is water, % is mass percentage content, prepared by sanding process; 10% WML-01 suspension agent, self-made, wherein WML-01 10%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, the balance is water, % is mass percentage content, prepared by sanding process; 40% difenoconazole suspension agent, product of Qingdao Audis Biotechnology Co., Ltd., Shandong Province.

[0086] 1.2 Test point profile The test was set in Daofasi Town, Wuxue City, Huanggang City, Hubei Province, and the soil texture was loam.

[0087] Rape variety Huayouzha 62, sowing amount 400 g / mu.

[0088] 1.3 Test design Table 15 Test scheme of fungicide treatment on sclerotinia stem rot of rape

[0089] 1.4 Investigation method and calculation Five-point sampling method was used, no less than 40 plants were sampled per point, 200 plants were investigated per plot, and the number of diseased plants and disease grade of each plot were recorded. Disease index and control effect were calculated.

[0090] The grading standard is as follows: 0 level: no disease; 1 level: the diseased area accounts for less than 5% of the main stem surface area; 3 level: the diseased area accounts for more than 5% but less than 15% of the main stem surface area; 5 level: the diseased area accounts for more than 15% but less than 30% of the main stem surface area; 7 level: the diseased area accounts for more than 30% but less than 50% of the main stem surface area; 9 level: the diseased area accounts for more than 50% of the main stem surface area.

[0091] Pharmacodynamic calculation method: Disease index (%) =∑(number of plants at each level × representative value at each level) / (total number of investigated plants × representative value at the highest level) × 100 Control effect (%) = (disease index after treatment in the control area - disease index after treatment in the treatment area) / disease index after treatment in the control area × 100 2 Test results Table 16 Control effect of fungicide treatment on sclerotinia stem rot of rape

[0092] Note: * The average of 4 times; ** The significance level of variance analysis is p=0.05.

[0093] From the above table, it can be seen that after 2 times of application, the control effect of 15% WML-01·difenoconazole suspension concentrate high dose 100 ml treatment (effective ingredient 15 g) and low dose 50 ml treatment (effective ingredient 7.5 g) on Sclerotinia sclerotiorum of rape is 89.96% and 82.76% respectively, which is significantly better than that of difenoconazole single agent treatment (effective ingredient 12 g) and WML-01 single agent treatment (effective ingredient 20 g).

[0094] The above results show that after the combination of WML-01 and difenoconazole, excellent control effect on Sclerotinia sclerotiorum of rape is shown, and the field application amount is significantly reduced.

[0095] Example 8 Field control effect of WML-01 and difenoconazole combination on pepper anthracnose 1 Materials and methods 1.1 Test materials 15% WML-01·difenoconazole suspension concentrate, self-made, wherein WML-01 5%, difenoconazole 10%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, the balance is water, % is mass percent, prepared by sand mill process; 10% WML-01 suspension concentrate, self-made, wherein WML-01 10%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, the balance is water, % is mass percent, prepared by sand mill process; 40% difenoconazole suspension concentrate, product of Qingdao Audis Biotechnology Co., Ltd., Shandong Province.

[0096] 1.2 Test site profile The test was conducted in Daixin Town, Fugao County, Zhoukou City, Henan Province, the soil texture was loam, and the pepper variety was Mibao 19.

[0097] 1.3 Test design Table 17 Test scheme of fungicide treatment on pepper anthracnose

[0098] 1.4 Investigation method and calculation 5 points were sampled in each plot, and 20 fruits were investigated in each point, and the percentage of lesion area to the whole fruit area was used for grading.

[0099] Grading method: 0 level: no lesion; Grade 1: the area of disease spot is less than 5% of the whole fruit area; Grade 3: the area of disease spot is more than or equal to 5% and less than 10% of the whole fruit area; Grade 5: the area of disease spot is more than or equal to 10% and less than 25% of the whole fruit area; Grade 7: the area of disease spot is more than or equal to 25% and less than 50% of the whole fruit area; Grade 9: the area of disease spot is more than or equal to 50% of the whole fruit area, or the fruit drop.

[0100] Pharmacodynamic calculation method: Disease index (%) =∑(number of disease fruit of each grade × representative value of each grade) / (total number of fruit surveyed × representative value of highest grade) × 100 Control effect (%) = (disease index after treatment in control area - disease index after treatment in treatment area) / disease index after treatment in control area × 100 2 Test results Table 18: Control effect of the agent treatment on pepper anthracnose

[0101] Note: * The average value of 4 repetitions; ** The significance level of variance analysis is p = 0.05.

[0102] From the content in the above table, it can be seen that after 2 times of spraying, the control effect of 15% WML-01·difenoconazole suspension concentrate high dose 100 ml treatment (effective ingredient 15 g) on pepper anthracnose is 88.04%, which is obviously better than that of difenoconazole single agent treatment (effective ingredient 16 g) and WML-01 single agent treatment (effective ingredient 20 g), and the control effect of low dose 50 ml (effective ingredient 7.5 g) treatment is 79.26%, which is equivalent to that of difenoconazole single agent treatment and WML-01 single agent treatment.

[0103] The above results show that after the compounding of WML-01 and difenoconazole, the control effect on pepper anthracnose is excellent at the same dose as the two single agents, and when the control effect on pepper anthracnose is equivalent to that of the two single agents, the field use amount is reduced.

[0104] Although the present application has been described in detail in the foregoing description with general principles and specific embodiments, some modifications or improvements can be made on the basis of the present application, which is obvious to those skilled in the art. Therefore, these modifications or improvements made on the basis of not deviating from the spirit of the present application, all belong to the scope of protection claimed by the present application.

Claims

1. A fungicidal composition comprising WML-01 and a triazole compound, characterized in that, The fungicidal composition consists of WML-01 and difenoconazole, the mass ratio of the WML-01 and difenoconazole is 10:1-1:10; the WML-01 is a compound shown in formula (I): 。 2. The bactericidal composition containing WML-01 and a triazole compound according to claim 1, characterized by, The mass ratio of the WML-01 and difenoconazole is 1:1-1:

10.

3. The bactericidal composition containing WML-01 and a triazole compound according to claim 1, characterized by, The mass ratio of the WML-01 and difenoconazole is 10:1-2:

1.

4. A bactericidal preparation, characterized by, The fungicidal preparation contains the fungicidal composition according to any one of claims 1-5.

5. The bactericidal preparation according to claim 4, characterized in that, The dosage form of the fungicidal preparation is seed coating agent, suspension agent, water emulsion, microemulsion, emulsifiable concentrate, microcapsule suspension preparation, nano preparation, granule, wettable powder or water dispersible granule.

6. Use of a fungicidal composition according to any one of claims 1 to 3 or a fungicidal formulation according to any one of claims 4 to 5 in the control of plant diseases, the plant disease being one or more of rice bakanae disease Fusarium fujikuroi , wheat foot rot Fusarium pseudograminearum , wheat scab Fusarium graminearum , soybean root rot Fusarium , pepper anthracnose Colletotrichum , Sclerotinia sclerotiorum and Sclerotinia homeocarpa Sclerotinia sclerotiorum .

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